Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40380.t1 | XP_003603541.2 | 90.3 | 124 | 12 | 0 | 1 | 124 | 1 | 124 | 3.80E-54 | 220.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40380.t1 | G7JCD9 | 90.3 | 124 | 12 | 0 | 1 | 124 | 23 | 146 | 2.7e-54 | 220.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene40380 | -0.803496 | 3.39E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene40380 | -0.844819 | 5.97E-59 | -1.69E-46 |
| MS.gene049419 | MS.gene40380 | 0.838596 | 2.62E-57 | -1.69E-46 |
| MS.gene049499 | MS.gene40380 | -0.819474 | 1.14E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene40380 | -0.824617 | 7.32E-54 | -1.69E-46 |
| MS.gene050105 | MS.gene40380 | -0.857149 | 1.99E-62 | -1.69E-46 |
| MS.gene050654 | MS.gene40380 | -0.815696 | 8.10E-52 | -1.69E-46 |
| MS.gene051122 | MS.gene40380 | -0.822088 | 2.86E-53 | -1.69E-46 |
| MS.gene051332 | MS.gene40380 | -0.814741 | 1.32E-51 | -1.69E-46 |
| MS.gene051924 | MS.gene40380 | 0.802904 | 4.50E-49 | -1.69E-46 |
| MS.gene051926 | MS.gene40380 | 0.801317 | 9.53E-49 | -1.69E-46 |
| MS.gene05216 | MS.gene40380 | -0.813478 | 2.51E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene40380 | -0.81301 | 3.18E-51 | -1.69E-46 |
| MS.gene052463 | MS.gene40380 | -0.805634 | 1.21E-49 | -1.69E-46 |
| MS.gene052534 | MS.gene40380 | -0.804507 | 2.09E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene40380 | -0.826334 | 2.87E-54 | -1.69E-46 |
| MS.gene052674 | MS.gene40380 | -0.830141 | 3.47E-55 | -1.69E-46 |
| MS.gene052675 | MS.gene40380 | -0.833787 | 4.36E-56 | -1.69E-46 |
| MS.gene052677 | MS.gene40380 | -0.832506 | 9.09E-56 | -1.69E-46 |
| MS.gene052775 | MS.gene40380 | -0.810108 | 1.36E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene40380 | -0.800089 | 1.70E-48 | -1.69E-46 |
| MS.gene054291 | MS.gene40380 | 0.808108 | 3.64E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene40380 | -0.865801 | 4.54E-65 | -1.69E-46 |
| MS.gene054576 | MS.gene40380 | -0.815411 | 9.37E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene40380 | -0.812595 | 3.92E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene40380 | -0.817532 | 3.14E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene40380 | -0.805013 | 1.64E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene40380 | -0.810321 | 1.22E-50 | -1.69E-46 |
| MS.gene055841 | MS.gene40380 | -0.800905 | 1.16E-48 | -1.69E-46 |
| MS.gene055864 | MS.gene40380 | -0.82004 | 8.46E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene40380 | -0.801207 | 1.00E-48 | -1.69E-46 |
| MS.gene056045 | MS.gene40380 | -0.851389 | 9.16E-61 | -1.69E-46 |
| MS.gene056480 | MS.gene40380 | -0.805226 | 1.48E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene40380 | -0.80745 | 5.03E-50 | -1.69E-46 |
| MS.gene056779 | MS.gene40380 | -0.815866 | 7.42E-52 | -1.69E-46 |
| MS.gene056973 | MS.gene40380 | -0.801638 | 8.19E-49 | -1.69E-46 |
| MS.gene057155 | MS.gene40380 | -0.825849 | 3.75E-54 | -1.69E-46 |
| MS.gene057156 | MS.gene40380 | 0.8046 | 2.00E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene40380 | -0.83493 | 2.25E-56 | -1.69E-46 |
| MS.gene057598 | MS.gene40380 | -0.806991 | 6.29E-50 | -1.69E-46 |
| MS.gene057855 | MS.gene40380 | -0.806467 | 8.11E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene40380 | -0.844476 | 7.38E-59 | -1.69E-46 |
| MS.gene058223 | MS.gene40380 | 0.859865 | 3.08E-63 | -1.69E-46 |
| MS.gene058289 | MS.gene40380 | -0.806427 | 8.27E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene40380 | -0.800436 | 1.44E-48 | -1.69E-46 |
| MS.gene059438 | MS.gene40380 | -0.805405 | 1.36E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene40380 | -0.832845 | 7.49E-56 | -1.69E-46 |
| MS.gene05985 | MS.gene40380 | 0.815007 | 1.15E-51 | -1.69E-46 |
| MS.gene060435 | MS.gene40380 | -0.836409 | 9.52E-57 | -1.69E-46 |
| MS.gene06052 | MS.gene40380 | -0.813525 | 2.45E-51 | -1.69E-46 |
| MS.gene060777 | MS.gene40380 | -0.82239 | 2.43E-53 | -1.69E-46 |
| MS.gene060850 | MS.gene40380 | -0.825443 | 4.67E-54 | -1.69E-46 |
| MS.gene060851 | MS.gene40380 | -0.843376 | 1.46E-58 | -1.69E-46 |
| MS.gene060852 | MS.gene40380 | -0.838434 | 2.88E-57 | -1.69E-46 |
| MS.gene060932 | MS.gene40380 | -0.82502 | 5.89E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene40380 | -0.80544 | 1.33E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene40380 | -0.816026 | 6.84E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene40380 | -0.828626 | 8.10E-55 | -1.69E-46 |
| MS.gene06121 | MS.gene40380 | -0.800044 | 1.73E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40380.t1 | MTR_3g108900 | 90.323 | 124 | 12 | 0 | 1 | 124 | 23 | 146 | 4.72e-71 | 211 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40380.t1 | AT5G10700 | 49.580 | 119 | 43 | 3 | 6 | 124 | 24 | 125 | 5.16e-33 | 114 |
| MS.gene40380.t1 | AT5G10700 | 67.901 | 81 | 23 | 2 | 45 | 124 | 47 | 125 | 5.91e-33 | 114 |
Find 38 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTTGACTCATTGAGTTAA+AGG | 0.322492 | 3.3:-89008431 | None:intergenic |
| CTGATCGATACTTGGCCATT+AGG | 0.378184 | 3.3:+89008544 | MS.gene40380:CDS |
| ACGCTTCTGTTTCCGCGGTT+TGG | 0.395935 | 3.3:+89008590 | MS.gene40380:CDS |
| AGTGGTGGAATCGGTGGAGG+CGG | 0.421097 | 3.3:-89008452 | None:intergenic |
| TGAGTAACCACACTGCCTAA+TGG | 0.453176 | 3.3:-89008559 | None:intergenic |
| GGTGACAGGATCGTCTTTGT+TGG | 0.456796 | 3.3:-89008614 | None:intergenic |
| TGGTCCAATACGTGGTGCTC+CGG | 0.468634 | 3.3:+89008515 | MS.gene40380:CDS |
| GAGAGTGGTGGAATCGGTGG+AGG | 0.477145 | 3.3:-89008455 | None:intergenic |
| TAAAGGTGAGCTGACTCGTC+CGG | 0.477990 | 3.3:-89008414 | None:intergenic |
| CTCCACATCAACTGATTCGC+CGG | 0.486687 | 3.3:+89008477 | MS.gene40380:CDS |
| CACGCCGGAGCACCACGTAT+TGG | 0.503895 | 3.3:-89008519 | None:intergenic |
| GGCGTGATCTGATCGATACT+TGG | 0.507820 | 3.3:+89008536 | MS.gene40380:CDS |
| CTACAATAATCAACGGTGAC+AGG | 0.516772 | 3.3:-89008628 | None:intergenic |
| TGAGAAGGCGAGGTACGAAC+GGG | 0.519329 | 3.3:-89008347 | None:intergenic |
| GTGGTGGAATCGGTGGAGGC+GGG | 0.520690 | 3.3:-89008451 | None:intergenic |
| TTGAGAAGGCGAGGTACGAA+CGG | 0.522363 | 3.3:-89008348 | None:intergenic |
| TGTGGAGAGAGTGGTGGAAT+CGG | 0.534342 | 3.3:-89008461 | None:intergenic |
| AGGCGAGGTACGAACGGGAG+GGG | 0.553596 | 3.3:-89008342 | None:intergenic |
| TTCCGGCGAATCAGTTGATG+TGG | 0.555932 | 3.3:-89008479 | None:intergenic |
| GGGTTGAAGAGACGAAGCGT+AGG | 0.561056 | 3.3:-89008322 | None:intergenic |
| TTAGGCAGTGTGGTTACTCA+AGG | 0.562328 | 3.3:+89008562 | MS.gene40380:CDS |
| GAAGGCGAGGTACGAACGGG+AGG | 0.562660 | 3.3:-89008344 | None:intergenic |
| GGAGAGAGTGGTGGAATCGG+TGG | 0.566146 | 3.3:-89008458 | None:intergenic |
| AAGGCGAGGTACGAACGGGA+GGG | 0.566860 | 3.3:-89008343 | None:intergenic |
| GCCGGAAAACGTCGATGTTG+TGG | 0.569743 | 3.3:+89008495 | MS.gene40380:CDS |
| TGAAGAGACGAAGCGTAGGA+AGG | 0.573707 | 3.3:-89008318 | None:intergenic |
| ACTAAACTTCGTTCTTGAGA+AGG | 0.577942 | 3.3:-89008362 | None:intergenic |
| GAAGAGACGAAGCGTAGGAA+GGG | 0.601157 | 3.3:-89008317 | None:intergenic |
| GTCGGGGCTACAATAATCAA+CGG | 0.604557 | 3.3:-89008635 | None:intergenic |
| ACTTCGTTCTTGAGAAGGCG+AGG | 0.622634 | 3.3:-89008357 | None:intergenic |
| TTGTCACGCTTCTGTTTCCG+CGG | 0.641384 | 3.3:+89008585 | MS.gene40380:CDS |
| ATCAGTTGATGTGGAGAGAG+TGG | 0.646257 | 3.3:-89008470 | None:intergenic |
| CGATGTTGTGGTCCAATACG+TGG | 0.652164 | 3.3:+89008507 | MS.gene40380:CDS |
| GTCTTTGTTGGACCAAACCG+CGG | 0.653891 | 3.3:-89008602 | None:intergenic |
| AGTTGATGTGGAGAGAGTGG+TGG | 0.659985 | 3.3:-89008467 | None:intergenic |
| TACTTGGCCATTAGGCAGTG+TGG | 0.667910 | 3.3:+89008552 | MS.gene40380:CDS |
| GTTTAGTGTCACTTTACACG+CGG | 0.717247 | 3.3:+89008378 | MS.gene40380:CDS |
| AAGTATCGATCAGATCACGC+CGG | 0.740521 | 3.3:-89008534 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTGCATAGAATCAATTTTGT+CGG | - | chr3.3:89008656-89008675 | None:intergenic | 30.0% |
| ! | TGCATAGAATCAATTTTGTC+GGG | - | chr3.3:89008655-89008674 | None:intergenic | 30.0% |
| ACTAAACTTCGTTCTTGAGA+AGG | - | chr3.3:89008365-89008384 | None:intergenic | 35.0% | |
| ! | GCATAGAATCAATTTTGTCG+GGG | - | chr3.3:89008654-89008673 | None:intergenic | 35.0% |
| CTACAATAATCAACGGTGAC+AGG | - | chr3.3:89008631-89008650 | None:intergenic | 40.0% | |
| ! | GGGTTGACTCATTGAGTTAA+AGG | - | chr3.3:89008434-89008453 | None:intergenic | 40.0% |
| !! | GTTTAGTGTCACTTTACACG+CGG | + | chr3.3:89008378-89008397 | MS.gene40380:CDS | 40.0% |
| CTGATCGATACTTGGCCATT+AGG | + | chr3.3:89008544-89008563 | MS.gene40380:CDS | 45.0% | |
| GTCGGGGCTACAATAATCAA+CGG | - | chr3.3:89008638-89008657 | None:intergenic | 45.0% | |
| TGAGTAACCACACTGCCTAA+TGG | - | chr3.3:89008562-89008581 | None:intergenic | 45.0% | |
| TTAGGCAGTGTGGTTACTCA+AGG | + | chr3.3:89008562-89008581 | MS.gene40380:CDS | 45.0% | |
| ! | ACCACAACATCGACGTTTTC+CGG | - | chr3.3:89008499-89008518 | None:intergenic | 45.0% |
| ! | ACTCGTCCGGTTTGAAAAAG+AGG | - | chr3.3:89008404-89008423 | None:intergenic | 45.0% |
| ! | ATCAGTTGATGTGGAGAGAG+TGG | - | chr3.3:89008473-89008492 | None:intergenic | 45.0% |
| !! | AAGTATCGATCAGATCACGC+CGG | - | chr3.3:89008537-89008556 | None:intergenic | 45.0% |
| ACTTCGTTCTTGAGAAGGCG+AGG | - | chr3.3:89008360-89008379 | None:intergenic | 50.0% | |
| CGATGTTGTGGTCCAATACG+TGG | + | chr3.3:89008507-89008526 | MS.gene40380:CDS | 50.0% | |
| GAAGAGACGAAGCGTAGGAA+GGG | - | chr3.3:89008320-89008339 | None:intergenic | 50.0% | |
| GGCGTGATCTGATCGATACT+TGG | + | chr3.3:89008536-89008555 | MS.gene40380:CDS | 50.0% | |
| GGTGACAGGATCGTCTTTGT+TGG | - | chr3.3:89008617-89008636 | None:intergenic | 50.0% | |
| GTCTTTGTTGGACCAAACCG+CGG | - | chr3.3:89008605-89008624 | None:intergenic | 50.0% | |
| TACTTGGCCATTAGGCAGTG+TGG | + | chr3.3:89008552-89008571 | MS.gene40380:CDS | 50.0% | |
| TGAAGAGACGAAGCGTAGGA+AGG | - | chr3.3:89008321-89008340 | None:intergenic | 50.0% | |
| TTGAGAAGGCGAGGTACGAA+CGG | - | chr3.3:89008351-89008370 | None:intergenic | 50.0% | |
| TTGTCACGCTTCTGTTTCCG+CGG | + | chr3.3:89008585-89008604 | MS.gene40380:CDS | 50.0% | |
| ! | AGTTGATGTGGAGAGAGTGG+TGG | - | chr3.3:89008470-89008489 | None:intergenic | 50.0% |
| ! | TGTGGAGAGAGTGGTGGAAT+CGG | - | chr3.3:89008464-89008483 | None:intergenic | 50.0% |
| ! | TTCCGGCGAATCAGTTGATG+TGG | - | chr3.3:89008482-89008501 | None:intergenic | 50.0% |
| !! | CTCCACATCAACTGATTCGC+CGG | + | chr3.3:89008477-89008496 | MS.gene40380:CDS | 50.0% |
| !! | TAAAGGTGAGCTGACTCGTC+CGG | - | chr3.3:89008417-89008436 | None:intergenic | 50.0% |
| !!! | ACGCGGCCTCTTTTTCAAAC+CGG | + | chr3.3:89008395-89008414 | MS.gene40380:CDS | 50.0% |
| ACGCTTCTGTTTCCGCGGTT+TGG | + | chr3.3:89008590-89008609 | MS.gene40380:CDS | 55.0% | |
| GCCGGAAAACGTCGATGTTG+TGG | + | chr3.3:89008495-89008514 | MS.gene40380:CDS | 55.0% | |
| GGGTTGAAGAGACGAAGCGT+AGG | - | chr3.3:89008325-89008344 | None:intergenic | 55.0% | |
| TGAGAAGGCGAGGTACGAAC+GGG | - | chr3.3:89008350-89008369 | None:intergenic | 55.0% | |
| TGGTCCAATACGTGGTGCTC+CGG | + | chr3.3:89008515-89008534 | MS.gene40380:CDS | 55.0% | |
| AAGGCGAGGTACGAACGGGA+GGG | - | chr3.3:89008346-89008365 | None:intergenic | 60.0% | |
| ! | AGTGGTGGAATCGGTGGAGG+CGG | - | chr3.3:89008455-89008474 | None:intergenic | 60.0% |
| ! | GAGAGTGGTGGAATCGGTGG+AGG | - | chr3.3:89008458-89008477 | None:intergenic | 60.0% |
| ! | GGAGAGAGTGGTGGAATCGG+TGG | - | chr3.3:89008461-89008480 | None:intergenic | 60.0% |
| AGGCGAGGTACGAACGGGAG+GGG | - | chr3.3:89008345-89008364 | None:intergenic | 65.0% | |
| GAAGGCGAGGTACGAACGGG+AGG | - | chr3.3:89008347-89008366 | None:intergenic | 65.0% | |
| ! | CACGCCGGAGCACCACGTAT+TGG | - | chr3.3:89008522-89008541 | None:intergenic | 65.0% |
| ! | GTGGTGGAATCGGTGGAGGC+GGG | - | chr3.3:89008454-89008473 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 89008307 | 89008678 | 89008307 | ID=MS.gene40380 |
| chr3.3 | mRNA | 89008307 | 89008678 | 89008307 | ID=MS.gene40380.t1;Parent=MS.gene40380 |
| chr3.3 | exon | 89008307 | 89008678 | 89008307 | ID=MS.gene40380.t1.exon1;Parent=MS.gene40380.t1 |
| chr3.3 | CDS | 89008307 | 89008678 | 89008307 | ID=cds.MS.gene40380.t1;Parent=MS.gene40380.t1 |
| Gene Sequence |
| Protein sequence |
