AlfalfaGEDB | MS.gene46434

M. sativa cultivar XinJiangDaYe / MS.gene46434

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46434.t1	XP_003590314.1	91.9	185	15	0	1	185	1	185	4.40E-91	344

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46434.t1	Q9SS03	49.3	152	73	3	36	185	40	189	4.1e-37	156.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46434.t1	G7I8H9	91.9	185	15	0	1	185	1	185	3.2e-91	344.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene46434	-0.810428	1.16E-50	-1.69E-46
MS.gene050380	MS.gene46434	0.856777	2.56E-62	-1.69E-46
MS.gene050381	MS.gene46434	0.830548	2.76E-55	-1.69E-46
MS.gene050647	MS.gene46434	0.800379	1.48E-48	-1.69E-46
MS.gene050809	MS.gene46434	0.826219	3.06E-54	-1.69E-46
MS.gene050868	MS.gene46434	-0.804609	1.99E-49	-1.69E-46
MS.gene050871	MS.gene46434	-0.807213	5.64E-50	-1.69E-46
MS.gene051138	MS.gene46434	0.816292	5.96E-52	-1.69E-46
MS.gene051632	MS.gene46434	0.810009	1.43E-50	-1.69E-46
MS.gene051863	MS.gene46434	0.807577	4.72E-50	-1.69E-46
MS.gene051996	MS.gene46434	0.803227	3.86E-49	-1.69E-46
MS.gene05234	MS.gene46434	0.800007	1.76E-48	-1.69E-46
MS.gene05240	MS.gene46434	0.802427	5.64E-49	-1.69E-46
MS.gene053406	MS.gene46434	0.831636	1.49E-55	-1.69E-46
MS.gene053420	MS.gene46434	0.807335	5.31E-50	-1.69E-46
MS.gene053421	MS.gene46434	0.825345	4.93E-54	-1.69E-46
MS.gene054003	MS.gene46434	-0.800586	1.34E-48	-1.69E-46
MS.gene054290	MS.gene46434	0.823174	1.60E-53	-1.69E-46
MS.gene054595	MS.gene46434	-0.821607	3.69E-53	-1.69E-46
MS.gene054706	MS.gene46434	0.8148	1.28E-51	-1.69E-46
MS.gene054742	MS.gene46434	0.820906	5.35E-53	-1.69E-46
MS.gene055124	MS.gene46434	0.822259	2.61E-53	-1.69E-46
MS.gene055154	MS.gene46434	0.808455	3.07E-50	-1.69E-46
MS.gene055202	MS.gene46434	0.826001	3.45E-54	-1.69E-46
MS.gene055577	MS.gene46434	-0.802502	5.44E-49	-1.69E-46
MS.gene055959	MS.gene46434	0.827522	1.49E-54	-1.69E-46
MS.gene056166	MS.gene46434	-0.802816	4.69E-49	-1.69E-46
MS.gene056313	MS.gene46434	0.810824	9.51E-51	-1.69E-46
MS.gene056590	MS.gene46434	0.811396	7.15E-51	-1.69E-46
MS.gene056591	MS.gene46434	0.81008	1.38E-50	-1.69E-46
MS.gene056856	MS.gene46434	0.807993	3.85E-50	-1.69E-46
MS.gene057544	MS.gene46434	0.809251	2.08E-50	-1.69E-46
MS.gene058093	MS.gene46434	0.841475	4.65E-58	-1.69E-46
MS.gene058099	MS.gene46434	0.82113	4.75E-53	-1.69E-46
MS.gene058109	MS.gene46434	0.819759	9.81E-53	-1.69E-46
MS.gene058170	MS.gene46434	0.817541	3.13E-52	-1.69E-46
MS.gene058171	MS.gene46434	0.852986	3.22E-61	-1.69E-46
MS.gene058173	MS.gene46434	0.849174	3.83E-60	-1.69E-46
MS.gene058174	MS.gene46434	0.816709	4.81E-52	-1.69E-46
MS.gene058175	MS.gene46434	0.851812	6.95E-61	-1.69E-46
MS.gene058177	MS.gene46434	0.809903	1.50E-50	-1.69E-46
MS.gene058179	MS.gene46434	0.833516	5.10E-56	-1.69E-46
MS.gene058180	MS.gene46434	0.831286	1.82E-55	-1.69E-46
MS.gene058840	MS.gene46434	0.822385	2.44E-53	-1.69E-46
MS.gene059037	MS.gene46434	0.826496	2.63E-54	-1.69E-46
MS.gene059038	MS.gene46434	0.815651	8.29E-52	-1.69E-46
MS.gene059045	MS.gene46434	0.837778	4.25E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46434.t1	MTR_1g056370	91.398	186	16	0	1	186	1	186	1.85e-125	351
MS.gene46434.t1	MTR_4g013330	48.052	154	76	2	36	186	47	199	1.87e-48	156
MS.gene46434.t1	MTR_4g078885	46.703	182	89	5	4	185	11	184	3.64e-48	155
MS.gene46434.t1	MTR_4g013320	45.238	168	84	4	22	186	37	199	2.41e-47	154
MS.gene46434.t1	MTR_3g034030	45.902	183	91	4	6	185	7	184	7.41e-47	152
MS.gene46434.t1	MTR_4g013350	47.059	153	77	3	36	186	47	197	5.72e-46	150
MS.gene46434.t1	MTR_4g013345	45.752	153	79	3	36	186	46	196	1.70e-45	149
MS.gene46434.t1	MTR_7g093830	43.850	187	91	5	2	186	19	193	5.20e-45	147
MS.gene46434.t1	MTR_7g093790	39.894	188	100	4	1	186	15	191	7.49e-45	147
MS.gene46434.t1	MTR_7g093820	46.203	158	82	3	31	186	38	194	1.12e-44	146
MS.gene46434.t1	MTR_4g013325	45.205	146	78	2	42	186	55	199	1.28e-44	146
MS.gene46434.t1	MTR_7g093850	43.316	187	92	5	2	186	20	194	1.32e-44	146
MS.gene46434.t1	MTR_1g054525	46.053	152	78	3	36	186	44	192	8.49e-43	142
MS.gene46434.t1	MTR_4g013385	40.642	187	98	5	7	186	10	190	2.26e-42	140
MS.gene46434.t1	MTR_0433s0040	40.642	187	98	5	7	186	10	190	2.26e-42	140
MS.gene46434.t1	MTR_4g013335	43.046	151	84	2	36	186	48	196	3.90e-42	140
MS.gene46434.t1	MTR_4g013770	45.860	157	79	5	32	185	37	190	7.85e-42	139
MS.gene46434.t1	MTR_4g073950	40.645	155	89	2	34	186	39	192	9.04e-42	139
MS.gene46434.t1	MTR_7g070390	41.711	187	100	4	7	186	8	192	1.68e-41	138
MS.gene46434.t1	MTR_3g105640	39.572	187	104	3	6	186	5	188	7.02e-40	134
MS.gene46434.t1	MTR_4g013310	42.667	150	82	3	38	186	51	197	4.55e-39	132
MS.gene46434.t1	MTR_3g105630	41.053	190	101	4	3	186	5	189	1.33e-38	131
MS.gene46434.t1	MTR_7g093870	41.615	161	89	2	31	186	33	193	3.25e-37	128
MS.gene46434.t1	MTR_5g096120	36.667	150	91	1	41	186	40	189	1.88e-33	117
MS.gene46434.t1	MTR_4g013355	37.908	153	83	3	35	186	38	179	2.55e-29	107
MS.gene46434.t1	MTR_4g122110	36.242	149	89	3	41	186	35	180	2.05e-24	94.4
MS.gene46434.t1	MTR_4g122130	35.811	148	91	3	41	186	35	180	2.99e-22	89.0
MS.gene46434.t1	MTR_4g049550	29.032	155	108	2	30	183	22	175	2.32e-19	81.3
MS.gene46434.t1	MTR_4g049570	33.566	143	91	3	43	183	35	175	1.22e-18	79.3
MS.gene46434.t1	MTR_8g099115	34.848	132	75	5	43	165	39	168	6.17e-14	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46434.t1	AT1G65870	50.000	152	72	3	36	185	40	189	3.40e-48	155
MS.gene46434.t1	AT3G13650	41.711	187	97	6	4	186	8	186	2.22e-47	153
MS.gene46434.t1	AT5G42510	48.344	151	74	3	36	185	35	182	3.60e-46	150
MS.gene46434.t1	AT5G42500	49.007	151	73	3	36	185	38	185	4.59e-45	147
MS.gene46434.t1	AT1G58170	43.671	158	85	3	31	186	30	185	3.39e-44	145
MS.gene46434.t1	AT1G22900	49.007	151	73	3	36	185	46	193	4.20e-44	145
MS.gene46434.t1	AT1G55210	46.405	153	75	4	36	186	40	187	2.86e-43	143
MS.gene46434.t1	AT1G55210	46.405	153	75	4	36	186	40	187	2.86e-43	143
MS.gene46434.t1	AT2G21100	42.466	146	82	2	41	185	43	187	2.75e-40	135
MS.gene46434.t1	AT2G21100	42.466	146	82	2	41	185	43	187	2.75e-40	135
MS.gene46434.t1	AT5G49040	39.634	164	95	3	24	186	31	191	1.32e-39	134
MS.gene46434.t1	AT3G13662	43.165	139	74	3	36	173	38	172	9.59e-36	123
MS.gene46434.t1	AT3G58090	47.101	138	63	3	53	185	139	271	4.88e-33	119
MS.gene46434.t1	AT2G21110	37.805	164	96	2	28	186	24	186	1.13e-32	115
MS.gene46434.t1	AT5G49030	43.511	131	72	2	36	165	41	170	2.65e-31	119
MS.gene46434.t1	AT3G13660	42.105	114	65	1	73	186	13	125	6.40e-31	109
MS.gene46434.t1	AT4G38700	35.897	156	92	4	38	186	36	190	1.78e-26	100
MS.gene46434.t1	AT4G23690	35.878	131	77	5	35	161	35	162	5.89e-17	75.1
MS.gene46434.t1	AT1G64160	33.898	118	71	4	46	159	41	155	1.56e-14	68.6
MS.gene46434.t1	AT4G11190	33.884	121	73	5	45	161	41	158	1.78e-13	65.9
MS.gene46434.t1	AT4G11210	30.488	164	108	5	2	165	5	162	2.92e-13	65.5

Find 43 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATAAGCTCTGAACTTAAATT+TGG	0.164549	1.2:+44401224	None:intergenic
GAATCATTTCATGGTCCTTT+TGG	0.201390	1.2:-44401284	MS.gene46434:CDS
AAATTTGGTTCTTTAGTTAA+AGG	0.202826	1.2:+44401239	None:intergenic
ATGCTTTGGCTAAGACTATT+TGG	0.206020	1.2:-44401004	MS.gene46434:CDS
TTTCTGAGAGTAAACTTATA+TGG	0.219169	1.2:+44401399	None:intergenic
GGTGAGGCTGCTCCCTTTGT+AGG	0.246807	1.2:+44401112	None:intergenic
GCTTGGGAGGAATGTTATTA+TGG	0.306263	1.2:-44401087	MS.gene46434:CDS
AAGAAGCAAGAGGCTGAAAA+TGG	0.322317	1.2:+44401475	None:intergenic
AATTTAAGTTCAGAGCTTAT+TGG	0.325685	1.2:-44401221	MS.gene46434:CDS
AGCCCAAGGAACCTATTCTT+TGG	0.331068	1.2:-44401195	MS.gene46434:CDS
AATTTGGTTCTTTAGTTAAA+GGG	0.362691	1.2:+44401240	None:intergenic
ATTTGGTATAATTCTACATC+TGG	0.367460	1.2:-44400987	MS.gene46434:CDS
TTCTGAGAGTAAACTTATAT+GGG	0.374193	1.2:+44401400	None:intergenic
CCACCCAATGAATCATTTCA+TGG	0.398006	1.2:-44401293	MS.gene46434:CDS
AGGACCATGAAATGATTCAT+TGG	0.400816	1.2:+44401289	None:intergenic
ATTTGCAAGGGGTTATGCTT+TGG	0.411059	1.2:-44401018	MS.gene46434:CDS
ATGCCTATTGTTGGAGGAAC+TGG	0.412156	1.2:-44401050	MS.gene46434:CDS
AATTACTTCATGAGTGTTCA+AGG	0.435174	1.2:-44401446	MS.gene46434:CDS
GGGAGCAGCCTCACCATGCT+TGG	0.438720	1.2:-44401104	MS.gene46434:CDS
GCACCAGTTCCTCCAACAAT+AGG	0.444460	1.2:+44401047	None:intergenic
GTTAGAGAAATGCCTATTGT+TGG	0.446792	1.2:-44401059	MS.gene46434:CDS
AAGCCAAAGAATAGGTTCCT+TGG	0.451718	1.2:+44401192	None:intergenic
CATGAAAATAAAAGTGTAAG+TGG	0.459702	1.2:+44401360	None:intergenic
AGCCAAAGAATAGGTTCCTT+GGG	0.475806	1.2:+44401193	None:intergenic
GGACCATGAAATGATTCATT+GGG	0.484202	1.2:+44401290	None:intergenic
TTGTTGAGAAGCCAAAGAAT+AGG	0.495361	1.2:+44401184	None:intergenic
GGAGCAGCCTCACCATGCTT+GGG	0.516739	1.2:-44401103	MS.gene46434:CDS
TTTACAGCTGGAACCTACAA+AGG	0.527260	1.2:-44401125	MS.gene46434:CDS
AGAGAAATGCCTATTGTTGG+AGG	0.533145	1.2:-44401056	MS.gene46434:CDS
TTACAGCTGGAACCTACAAA+GGG	0.548749	1.2:-44401124	MS.gene46434:CDS
CTGGTGCTTTCAGATTTGCA+AGG	0.550900	1.2:-44401031	MS.gene46434:CDS
AGTATGTAAGTGGCTCCAAA+AGG	0.553653	1.2:+44401269	None:intergenic
TGGTGCTTTCAGATTTGCAA+GGG	0.553765	1.2:-44401030	MS.gene46434:CDS
CCATGAAATGATTCATTGGG+TGG	0.554764	1.2:+44401293	None:intergenic
AGGGTTGTCTAGTATGTAAG+TGG	0.563851	1.2:+44401259	None:intergenic
TATTCTTTGGCTTCTCAACA+AGG	0.573141	1.2:-44401182	MS.gene46434:CDS
ATGAAAATAAAAGTGTAAGT+GGG	0.573723	1.2:+44401361	None:intergenic
GAGCTTATTGGAAGAGCCCA+AGG	0.578477	1.2:-44401209	MS.gene46434:CDS
GCAGCCTCACCATGCTTGGG+AGG	0.595405	1.2:-44401100	MS.gene46434:CDS
AATAACATTCCTCCCAAGCA+TGG	0.596205	1.2:+44401091	None:intergenic
AATTACTGAAAAGAAGCAAG+AGG	0.621735	1.2:+44401465	None:intergenic
CATTCCTCCCAAGCATGGTG+AGG	0.641930	1.2:+44401096	None:intergenic
GGTGCTTTCAGATTTGCAAG+GGG	0.678373	1.2:-44401029	MS.gene46434:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTGGTTCTTTAGTTAA+AGG	+	chr1.2:44401190-44401209	None:intergenic	20.0%
!!!	AATTTGGTTCTTTAGTTAAA+GGG	+	chr1.2:44401189-44401208	None:intergenic	20.0%
!!!	ATGAAAATAAAAGTGTAAGT+GGG	+	chr1.2:44401068-44401087	None:intergenic	20.0%
!!!	ATTAATTTTGTTTTTACAGC+TGG	-	chr1.2:44401289-44401308	MS.gene46434:CDS	20.0%
!	ATTTGGTATAATTCTACATC+TGG	-	chr1.2:44401439-44401458	MS.gene46434:CDS	25.0%
!!	AATTTAAGTTCAGAGCTTAT+TGG	-	chr1.2:44401205-44401224	MS.gene46434:CDS	25.0%
!!	CATGAAAATAAAAGTGTAAG+TGG	+	chr1.2:44401069-44401088	None:intergenic	25.0%
!!	TTCTGAGAGTAAACTTATAT+GGG	+	chr1.2:44401029-44401048	None:intergenic	25.0%
!!	TTTCTGAGAGTAAACTTATA+TGG	+	chr1.2:44401030-44401049	None:intergenic	25.0%
!!!	ATAAGCTCTGAACTTAAATT+TGG	+	chr1.2:44401205-44401224	None:intergenic	25.0%
!!!	TTGATAATTTTGAGTGTAGT+TGG	+	chr1.2:44401112-44401131	None:intergenic	25.0%
	AATTACTGAAAAGAAGCAAG+AGG	+	chr1.2:44400964-44400983	None:intergenic	30.0%
	AATTACTTCATGAGTGTTCA+AGG	-	chr1.2:44400980-44400999	MS.gene46434:CDS	30.0%
!	TATTTTCATGACATCCTAGA+TGG	-	chr1.2:44401079-44401098	MS.gene46434:CDS	30.0%
	AGGACCATGAAATGATTCAT+TGG	+	chr1.2:44401140-44401159	None:intergenic	35.0%
	ATGCTTTGGCTAAGACTATT+TGG	-	chr1.2:44401422-44401441	MS.gene46434:CDS	35.0%
	GGACCATGAAATGATTCATT+GGG	+	chr1.2:44401139-44401158	None:intergenic	35.0%
	GTTAGAGAAATGCCTATTGT+TGG	-	chr1.2:44401367-44401386	MS.gene46434:CDS	35.0%
	TTGTTGAGAAGCCAAAGAAT+AGG	+	chr1.2:44401245-44401264	None:intergenic	35.0%
!	GAATCATTTCATGGTCCTTT+TGG	-	chr1.2:44401142-44401161	MS.gene46434:CDS	35.0%
!	TATTCTTTGGCTTCTCAACA+AGG	-	chr1.2:44401244-44401263	MS.gene46434:CDS	35.0%
!!!	TAGTTGGTTTTTCACCATCT+AGG	+	chr1.2:44401096-44401115	None:intergenic	35.0%
	AAGAAGCAAGAGGCTGAAAA+TGG	+	chr1.2:44400954-44400973	None:intergenic	40.0%
	AAGCCAAAGAATAGGTTCCT+TGG	+	chr1.2:44401237-44401256	None:intergenic	40.0%
	AATAACATTCCTCCCAAGCA+TGG	+	chr1.2:44401338-44401357	None:intergenic	40.0%
	AGAGAAATGCCTATTGTTGG+AGG	-	chr1.2:44401370-44401389	MS.gene46434:CDS	40.0%
	AGCCAAAGAATAGGTTCCTT+GGG	+	chr1.2:44401236-44401255	None:intergenic	40.0%
	AGGGTTGTCTAGTATGTAAG+TGG	+	chr1.2:44401170-44401189	None:intergenic	40.0%
	AGTATGTAAGTGGCTCCAAA+AGG	+	chr1.2:44401160-44401179	None:intergenic	40.0%
	ATTTGCAAGGGGTTATGCTT+TGG	-	chr1.2:44401408-44401427	MS.gene46434:CDS	40.0%
	CCACCCAATGAATCATTTCA+TGG	-	chr1.2:44401133-44401152	MS.gene46434:CDS	40.0%
	CCATGAAATGATTCATTGGG+TGG	+	chr1.2:44401136-44401155	None:intergenic	40.0%
	GCTTGGGAGGAATGTTATTA+TGG	-	chr1.2:44401339-44401358	MS.gene46434:CDS	40.0%
	TGGTGCTTTCAGATTTGCAA+GGG	-	chr1.2:44401396-44401415	MS.gene46434:CDS	40.0%
	TTACAGCTGGAACCTACAAA+GGG	-	chr1.2:44401302-44401321	MS.gene46434:CDS	40.0%
	TTTACAGCTGGAACCTACAA+AGG	-	chr1.2:44401301-44401320	MS.gene46434:CDS	40.0%
	AGCCCAAGGAACCTATTCTT+TGG	-	chr1.2:44401231-44401250	MS.gene46434:CDS	45.0%
	ATGCCTATTGTTGGAGGAAC+TGG	-	chr1.2:44401376-44401395	MS.gene46434:CDS	45.0%
	CTGGTGCTTTCAGATTTGCA+AGG	-	chr1.2:44401395-44401414	MS.gene46434:CDS	45.0%
	GGTGCTTTCAGATTTGCAAG+GGG	-	chr1.2:44401397-44401416	MS.gene46434:CDS	45.0%
	GAGCTTATTGGAAGAGCCCA+AGG	-	chr1.2:44401217-44401236	MS.gene46434:CDS	50.0%
	GCACCAGTTCCTCCAACAAT+AGG	+	chr1.2:44401382-44401401	None:intergenic	50.0%
	CATTCCTCCCAAGCATGGTG+AGG	+	chr1.2:44401333-44401352	None:intergenic	55.0%
	GGAGCAGCCTCACCATGCTT+GGG	-	chr1.2:44401323-44401342	MS.gene46434:CDS	60.0%
	GGTGAGGCTGCTCCCTTTGT+AGG	+	chr1.2:44401317-44401336	None:intergenic	60.0%
	GCAGCCTCACCATGCTTGGG+AGG	-	chr1.2:44401326-44401345	MS.gene46434:CDS	65.0%
	GGGAGCAGCCTCACCATGCT+TGG	-	chr1.2:44401322-44401341	MS.gene46434:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	44400944	44401504	44400944	ID=MS.gene46434
chr1.2	mRNA	44400944	44401504	44400944	ID=MS.gene46434.t1;Parent=MS.gene46434
chr1.2	exon	44400944	44401504	44400944	ID=MS.gene46434.t1.exon1;Parent=MS.gene46434.t1
chr1.2	CDS	44400944	44401504	44400944	ID=cds.MS.gene46434.t1;Parent=MS.gene46434.t1

Gene Sequence

>MS.gene46434

ATGGTTTCCATTTTCAGCCTCTTGCTTCTTTTCAGTAATTACTTCATGAGTGTTCAAGGAAGAGTAGCGTCTTTAGAAGAATCCCATATAAGTTTACTCTCAGAAAAACTACAAAGTTTTACCCACTTACACTTTTATTTTCATGACATCCTAGATGGTGAAAAACCAACTACACTCAAAATTATCAATCCACCCAATGAATCATTTCATGGTCCTTTTGGAGCCACTTACATACTAGACAACCCTTTAACTAAAGAACCAAATTTAAGTTCAGAGCTTATTGGAAGAGCCCAAGGAACCTATTCTTTGGCTTCTCAACAAGGTGATTTTGCATTTAAGATCGATATTAATTTTGTTTTTACAGCTGGAACCTACAAAGGGAGCAGCCTCACCATGCTTGGGAGGAATGTTATTATGGATGAAGTTAGAGAAATGCCTATTGTTGGAGGAACTGGTGCTTTCAGATTTGCAAGGGGTTATGCTTTGGCTAAGACTATTTGGTATAATTCTACATCTGGAAATGCTATTGAAGAGTTTGATATTATAATTTTACATTTCTAA

Protein sequence

>MS.gene46434.t1

MVSIFSLLLLFSNYFMSVQGRVASLEESHISLLSEKLQSFTHLHFYFHDILDGEKPTTLKIINPPNESFHGPFGATYILDNPLTKEPNLSSELIGRAQGTYSLASQQGDFAFKIDINFVFTAGTYKGSSLTMLGRNVIMDEVREMPIVGGTGAFRFARGYALAKTIWYNSTSGNAIEEFDIIILHF