AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50546


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50546.t1 MTR_1g071630 91.925 161 6 1 1 154 5 165 8.31e-84 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50546.t1 AT4G04614 56.589 129 51 3 27 154 52 176 9.27e-42 137
MS.gene50546.t1 AT4G04614 56.589 129 51 3 27 154 52 176 9.27e-42 137

Find 35 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTGTGTCATCTTCTT+TGG 0.118453 1.2:-54733665 None:intergenic
TTCTTTGTGTCATCTTCTTT+GGG 0.233840 1.2:-54733664 None:intergenic
AGGGACAATGGAGTTTATTC+TGG 0.281268 1.2:+54733919 MS.gene50546:CDS
CTGAAGCGATACGGCTATAA+AGG 0.293708 1.2:+54733613 MS.gene50546:CDS
TAAATCTACAAAGCAGAAAA+AGG 0.315518 1.2:+54733570 MS.gene50546:CDS
AGGCGGACCTTCAGTCTTAA+AGG 0.331123 1.2:+54733633 MS.gene50546:CDS
TCTGAAAATGATAGTGAAAC+TGG 0.358513 1.2:+54732470 MS.gene50546:CDS
CTCAGAAGGAAAAGAAGAAA+AGG 0.400791 1.2:+54733839 MS.gene50546:CDS
GGGATCTTGGTCAAGCAAGC+AGG 0.419342 1.2:+54733860 MS.gene50546:CDS
GAAAAGAAGAAAAGGGATCT+TGG 0.429119 1.2:+54733847 MS.gene50546:CDS
TTCAGAGCTTCAAAGTCAAT+TGG 0.442566 1.2:-54733595 None:intergenic
AAGATGACACAAAGAAAGAT+TGG 0.453949 1.2:+54733671 MS.gene50546:CDS
GTGTCATCTTCTTTGGGTGG+AGG 0.460313 1.2:-54733658 None:intergenic
CAGTTGGCATTTGCTCTCCT+AGG 0.493250 1.2:-54733780 None:intergenic
ATTATGTTGAAGAAGAGAAA+AGG 0.499802 1.2:+54733890 MS.gene50546:CDS
GGATCTTGGTCAAGCAAGCA+GGG 0.515240 1.2:+54733861 MS.gene50546:CDS
TTTGTGTCATCTTCTTTGGG+TGG 0.516808 1.2:-54733661 None:intergenic
TCAGAAGGAAAAGAAGAAAA+GGG 0.533332 1.2:+54733840 MS.gene50546:CDS
AGAAGAGAAAAGGCTGTTGA+GGG 0.535745 1.2:+54733900 MS.gene50546:CDS
TCATTCCTAGTCAGAACAGT+TGG 0.556209 1.2:-54733796 None:intergenic
ACACAAAGAAAGATTGGTCC+TGG 0.556354 1.2:+54733677 MS.gene50546:CDS
ACAAGAGAGGCTCTTTCCCT+AGG 0.557271 1.2:+54733763 MS.gene50546:CDS
AAAAGGCTGTTGAGGGACAA+TGG 0.563867 1.2:+54733907 MS.gene50546:CDS
TTTGAAGCTCTGAAGCGATA+CGG 0.565621 1.2:+54733604 MS.gene50546:CDS
TTGGTCCTGGTCCAGTGGAA+AGG 0.567873 1.2:+54733690 MS.gene50546:CDS
AGTTGGCATTTGCTCTCCTA+GGG 0.578927 1.2:-54733779 None:intergenic
AAGAAGAGAAAAGGCTGTTG+AGG 0.580825 1.2:+54733899 MS.gene50546:CDS
AAAGATTGGTCCTGGTCCAG+TGG 0.594354 1.2:+54733685 MS.gene50546:CDS
GGAGGTACCTTTAAGACTGA+AGG 0.597747 1.2:-54733640 None:intergenic
AAATGCCAACTGTTCTGACT+AGG 0.603014 1.2:+54733791 MS.gene50546:CDS
CTATGAAGAGGACAATGCCA+TGG 0.617111 1.2:+54732417 None:intergenic
ATCTTGGTCAAGCAAGCAGG+GGG 0.627378 1.2:+54733863 MS.gene50546:CDS
GATCTTGGTCAAGCAAGCAG+GGG 0.638659 1.2:+54733862 MS.gene50546:CDS
AGAGAGAAAGAAAACAAGAG+AGG 0.657420 1.2:+54733750 MS.gene50546:CDS
AAGCGATACGGCTATAAAGG+CGG 0.718806 1.2:+54733616 MS.gene50546:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGTAAATTAGAATATCG+GGG + chr1.2:54733329-54733348 MS.gene50546:intron 20.0%
!! AAGAACTATTATAGCATTAT+CGG - chr1.2:54733240-54733259 None:intergenic 20.0%
!! AGACAATAATATATTGCTAA+CGG - chr1.2:54733366-54733385 None:intergenic 20.0%
!! ATAATCACATTAAGAAAGAA+CGG - chr1.2:54732819-54732838 None:intergenic 20.0%
!! ATATAACTCAACATAGTTAT+AGG - chr1.2:54732532-54732551 None:intergenic 20.0%
!! ATATTCTAATTTACATTTCC+TGG - chr1.2:54733327-54733346 None:intergenic 20.0%
!! GAAATCTAATGTGATAAATT+AGG + chr1.2:54732972-54732991 MS.gene50546:intron 20.0%
!! GAAATGTAAATTAGAATATC+GGG + chr1.2:54733328-54733347 MS.gene50546:intron 20.0%
!! GGAAATGTAAATTAGAATAT+CGG + chr1.2:54733327-54733346 MS.gene50546:intron 20.0%
!!! ATTGTTTTAGATTTTGTTGT+TGG + chr1.2:54733122-54733141 MS.gene50546:intron 20.0%
!!! TTGTTTTAGATTTTGTTGTT+GGG + chr1.2:54733123-54733142 MS.gene50546:intron 20.0%
! AAAACACAAACACTAATTGT+GGG - chr1.2:54732852-54732871 None:intergenic 25.0%
! AGTGATAGCTGATAATATTA+TGG + chr1.2:54733023-54733042 MS.gene50546:intron 25.0%
! ATTATGTTGAAGAAGAGAAA+AGG + chr1.2:54733890-54733909 MS.gene50546:CDS 25.0%
! TAAATCTACAAAGCAGAAAA+AGG + chr1.2:54733570-54733589 MS.gene50546:CDS 25.0%
! TGACAAGTGAAAATAGTATT+TGG + chr1.2:54732756-54732775 MS.gene50546:intron 25.0%
! TGTTGAGTTATATGTTTAAG+TGG + chr1.2:54732539-54732558 MS.gene50546:intron 25.0%
!! ACAATTAGTGTTTGTGTTTT+GGG + chr1.2:54732852-54732871 MS.gene50546:intron 25.0%
!! ATTATCTGAAGATCATTTTG+TGG + chr1.2:54732947-54732966 MS.gene50546:intron 25.0%
!!! ACACTTTTAATCTGAAGTAT+CGG + chr1.2:54732719-54732738 MS.gene50546:intron 25.0%
!!! CAAAAAATCCAAAGGTATTT+TGG + chr1.2:54732496-54732515 MS.gene50546:intron 25.0%
!!! CAAAATACCTTTGGATTTTT+TGG - chr1.2:54732498-54732517 None:intergenic 25.0%
!!! TGTTTTAGATTTTGTTGTTG+GGG + chr1.2:54733124-54733143 MS.gene50546:intron 25.0%
AAATGTACTTACTTGAACCT+AGG + chr1.2:54732781-54732800 MS.gene50546:intron 30.0%
AAGATGACACAAAGAAAGAT+TGG + chr1.2:54733671-54733690 MS.gene50546:CDS 30.0%
ACCAATAACAAGGAAATACA+AGG - chr1.2:54733091-54733110 None:intergenic 30.0%
ACTAGTAACTAAGCAACATA+TGG - chr1.2:54733527-54733546 None:intergenic 30.0%
ATCACAATACACCAATAACA+AGG - chr1.2:54733101-54733120 None:intergenic 30.0%
ATGTGATAAATTAGGAGTTC+TGG + chr1.2:54732980-54732999 MS.gene50546:intron 30.0%
CAAAACACAAACACTAATTG+TGG - chr1.2:54732853-54732872 None:intergenic 30.0%
CAATAATATATTGCTAACGG+AGG - chr1.2:54733363-54733382 None:intergenic 30.0%
GATACTTCAGATTAAAAGTG+TGG - chr1.2:54732720-54732739 None:intergenic 30.0%
GATTTATGTAACACTAGCAA+CGG + chr1.2:54732605-54732624 MS.gene50546:intron 30.0%
GCTAATTAAGTCACACAATA+TGG - chr1.2:54733397-54733416 None:intergenic 30.0%
TCAGAAGGAAAAGAAGAAAA+GGG + chr1.2:54733840-54733859 MS.gene50546:CDS 30.0%
TTCTTTGTGTCATCTTCTTT+GGG - chr1.2:54733667-54733686 None:intergenic 30.0%
TTTCTTTGTGTCATCTTCTT+TGG - chr1.2:54733668-54733687 None:intergenic 30.0%
! AACTAGAGTAGATATTTTCC+AGG + chr1.2:54733306-54733325 MS.gene50546:intron 30.0%
! CACAATTAGTGTTTGTGTTT+TGG + chr1.2:54732851-54732870 MS.gene50546:intron 30.0%
! TCCTTGTATTTCCTTGTTAT+TGG + chr1.2:54733087-54733106 MS.gene50546:intron 30.0%
! TCTGAAAATGATAGTGAAAC+TGG + chr1.2:54732470-54732489 MS.gene50546:CDS 30.0%
!! AAAATCTCATCTCTAGAATG+TGG + chr1.2:54732886-54732905 MS.gene50546:intron 30.0%
AAGGAATCTCTTCTACAATG+GGG - chr1.2:54733072-54733091 None:intergenic 35.0%
AATCTAATCGTGTGTAGTGT+TGG + chr1.2:54732684-54732703 MS.gene50546:intron 35.0%
ACAAGGAATCTCTTCTACAA+TGG - chr1.2:54733074-54733093 None:intergenic 35.0%
AGAGAGAAAGAAAACAAGAG+AGG + chr1.2:54733750-54733769 MS.gene50546:CDS 35.0%
AGATGGTTCATAAACAGTTG+TGG + chr1.2:54733187-54733206 MS.gene50546:intron 35.0%
AGTATGAAGCCATCATGATA+CGG - chr1.2:54733455-54733474 None:intergenic 35.0%
CAAGAGTTTCTTGACTTTCA+AGG + chr1.2:54733282-54733301 MS.gene50546:intron 35.0%
CAAGGAATCTCTTCTACAAT+GGG - chr1.2:54733073-54733092 None:intergenic 35.0%
CTCAGAAGGAAAAGAAGAAA+AGG + chr1.2:54733839-54733858 MS.gene50546:CDS 35.0%
GAAAAGAAGAAAAGGGATCT+TGG + chr1.2:54733847-54733866 MS.gene50546:CDS 35.0%
TTAACTTACTTCATGTCTGC+AGG + chr1.2:54733547-54733566 MS.gene50546:intron 35.0%
TTCAGAGCTTCAAAGTCAAT+TGG - chr1.2:54733598-54733617 None:intergenic 35.0%
! ATAGCATTATCGGCTGATTT+AGG - chr1.2:54733230-54733249 None:intergenic 35.0%
! GAATCTTTCCTTTTCTCAGA+AGG + chr1.2:54733825-54733844 MS.gene50546:CDS 35.0%
!! CTTCTTTTCCTTCTGAGAAA+AGG - chr1.2:54733836-54733855 None:intergenic 35.0%
!! TTCTGAAATCCGTATCATGA+TGG + chr1.2:54733443-54733462 MS.gene50546:intron 35.0%
AAATGCCAACTGTTCTGACT+AGG + chr1.2:54733791-54733810 MS.gene50546:CDS 40.0%
AACTAAGCAACATATGGCAG+TGG - chr1.2:54733521-54733540 None:intergenic 40.0%
AAGAAGAGAAAAGGCTGTTG+AGG + chr1.2:54733899-54733918 MS.gene50546:CDS 40.0%
ACTAACTGAAGGTCAAGATG+GGG - chr1.2:54733159-54733178 None:intergenic 40.0%
ACTGGAGCCAAAAAATCCAA+AGG + chr1.2:54732488-54732507 MS.gene50546:CDS 40.0%
AGAAGAGAAAAGGCTGTTGA+GGG + chr1.2:54733900-54733919 MS.gene50546:CDS 40.0%
AGGGACAATGGAGTTTATTC+TGG + chr1.2:54733919-54733938 MS.gene50546:CDS 40.0%
CACTAACTGAAGGTCAAGAT+GGG - chr1.2:54733160-54733179 None:intergenic 40.0%
CTTGAACCTAGGATGTTGAT+AGG + chr1.2:54732792-54732811 MS.gene50546:intron 40.0%
GATGCACACCAAAATACCTT+TGG - chr1.2:54732507-54732526 None:intergenic 40.0%
TCAGTTAGTGCAATGCAAGA+TGG + chr1.2:54733170-54733189 MS.gene50546:intron 40.0%
TCATCATCATCATCACTCCA+TGG - chr1.2:54732437-54732456 None:intergenic 40.0%
TCATTCCTAGTCAGAACAGT+TGG - chr1.2:54733799-54733818 None:intergenic 40.0%
TCTTGCATTGCACTAACTGA+AGG - chr1.2:54733170-54733189 None:intergenic 40.0%
TTCAGATAATTGCAACGCAG+AGG - chr1.2:54732937-54732956 None:intergenic 40.0%
TTTGTGTCATCTTCTTTGGG+TGG - chr1.2:54733664-54733683 None:intergenic 40.0%
! ACACAAAGAAAGATTGGTCC+TGG + chr1.2:54733677-54733696 MS.gene50546:CDS 40.0%
!! TTTGAAGCTCTGAAGCGATA+CGG + chr1.2:54733604-54733623 MS.gene50546:CDS 40.0%
AAAAGGCTGTTGAGGGACAA+TGG + chr1.2:54733907-54733926 MS.gene50546:CDS 45.0%
AAAGGAAAAACGCGTCGAGA+AGG + chr1.2:54733708-54733727 MS.gene50546:CDS 45.0%
AACGCAGAGGTTTCATACCT+TGG - chr1.2:54732924-54732943 None:intergenic 45.0%
AACGGACCTATCAACATCCT+AGG - chr1.2:54732801-54732820 None:intergenic 45.0%
AAGCGATACGGCTATAAAGG+CGG + chr1.2:54733616-54733635 MS.gene50546:CDS 45.0%
AGTTGGCATTTGCTCTCCTA+GGG - chr1.2:54733782-54733801 None:intergenic 45.0%
CTGAAGCGATACGGCTATAA+AGG + chr1.2:54733613-54733632 MS.gene50546:CDS 45.0%
GCACTAACTGAAGGTCAAGA+TGG - chr1.2:54733161-54733180 None:intergenic 45.0%
TGACACTGACACTTCGATGT+TGG + chr1.2:54732634-54732653 MS.gene50546:intron 45.0%
! TTTTTCCTTTCCACTGGACC+AGG - chr1.2:54733698-54733717 None:intergenic 45.0%
!! GGAGGTACCTTTAAGACTGA+AGG - chr1.2:54733643-54733662 None:intergenic 45.0%
!! TCGTGTGTAGTGTTGGTGTA+CGG + chr1.2:54732691-54732710 MS.gene50546:intron 45.0%
ACAAGAGAGGCTCTTTCCCT+AGG + chr1.2:54733763-54733782 MS.gene50546:CDS 50.0%
AGGCGGACCTTCAGTCTTAA+AGG + chr1.2:54733633-54733652 MS.gene50546:CDS 50.0%
ATCTTGGTCAAGCAAGCAGG+GGG + chr1.2:54733863-54733882 MS.gene50546:CDS 50.0%
CAGTTGGCATTTGCTCTCCT+AGG - chr1.2:54733783-54733802 None:intergenic 50.0%
GATCTTGGTCAAGCAAGCAG+GGG + chr1.2:54733862-54733881 MS.gene50546:CDS 50.0%
GGATCTTGGTCAAGCAAGCA+GGG + chr1.2:54733861-54733880 MS.gene50546:CDS 50.0%
GTGTCATCTTCTTTGGGTGG+AGG - chr1.2:54733661-54733680 None:intergenic 50.0%
! AAAGATTGGTCCTGGTCCAG+TGG + chr1.2:54733685-54733704 MS.gene50546:CDS 50.0%
! GACGCGTTTTTCCTTTCCAC+TGG - chr1.2:54733704-54733723 None:intergenic 50.0%
! TGTGGATTGACGCTCATCCA+AGG + chr1.2:54732904-54732923 MS.gene50546:intron 50.0%
GGGATCTTGGTCAAGCAAGC+AGG + chr1.2:54733860-54733879 MS.gene50546:CDS 55.0%
TTGGTCCTGGTCCAGTGGAA+AGG + chr1.2:54733690-54733709 MS.gene50546:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 54732431 54733954 54732431 ID=MS.gene50546
chr1.2 mRNA 54732431 54733954 54732431 ID=MS.gene50546.t1;Parent=MS.gene50546
chr1.2 exon 54732431 54732509 54732431 ID=MS.gene50546.t1.exon1;Parent=MS.gene50546.t1
chr1.2 CDS 54732431 54732509 54732431 ID=cds.MS.gene50546.t1;Parent=MS.gene50546.t1
chr1.2 exon 54733569 54733954 54733569 ID=MS.gene50546.t1.exon2;Parent=MS.gene50546.t1
chr1.2 CDS 54733569 54733954 54733569 ID=cds.MS.gene50546.t1;Parent=MS.gene50546.t1
Gene Sequence

>MS.gene50546

ATGCCATGGAGTGATGATGATGATGACTCATCTTCAGACTCTGAAAATGATAGTGAAACTGGAGCCAAAAAATCCAAAGGTATTTTGGTGTGCATCATCCTATAACTATGTTGAGTTATATGTTTAAGTGGAGTATTTTTTGAGTATTTTGTTGACTACGATGAATTCATGTGTGATTTATGTAACACTAGCAACGGACATGATGACACTGACACTTCGATGTTGGTACTAATTTGAGAAAATAAATTAATTGAATCTAATCGTGTGTAGTGTTGGTGTACGGACACCACACTTTTAATCTGAAGTATCGGTGCTACAAAGTGTTTGACAAGTGAAAATAGTATTTGGACAAATGTACTTACTTGAACCTAGGATGTTGATAGGTCCGTTCTTTCTTAATGTGATTATAAGTTTATAACCCACAATTAGTGTTTGTGTTTTGGGTCTATTTAATAAAAATCTCATCTCTAGAATGTGGATTGACGCTCATCCAAGGTATGAAACCTCTGCGTTGCAATTATCTGAAGATCATTTTGTGGCTGAAATCTAATGTGATAAATTAGGAGTTCTGGCGAAAACTAAAAGTTAATTGAGTGATAGCTGATAATATTATGGAGAAATTGCACAATTTCATATTGCCCCATTGTAGAAGAGATTCCTTGTATTTCCTTGTTATTGGTGTATTGTGATCATTGTTTTAGATTTTGTTGTTGGGGATATATATACCCCATCTTGACCTTCAGTTAGTGCAATGCAAGATGGTTCATAAACAGTTGTGGTAGATACATAATGATATCCTAAATCAGCCGATAATGCTATAATAGTTCTTTTTATCTTCACAAAAATTCTTTCAAGAGTTTCTTGACTTTCAAGGCAACTAGAGTAGATATTTTCCAGGAAATGTAAATTAGAATATCGGGGTTGTTATACCTCCGTTAGCAATATATTATTGTCTTTGTTTGACCATATTGTGTGACTTAATTAGCGTTAGTTCATAATTGAAATTGTTAGCTTCTGAAATCCGTATCATGATGGCTTCATACTCATTTGCTTGTAAACTGTGACATCTTGAAAATTCTCACTAATGCCACTGCCATATGTTGCTTAGTTACTAGTTTAACTTACTTCATGTCTGCAGGTAAATCTACAAAGCAGAAAAAGGCTCCAATTGACTTTGAAGCTCTGAAGCGATACGGCTATAAAGGCGGACCTTCAGTCTTAAAGGTACCTCCACCCAAAGAAGATGACACAAAGAAAGATTGGTCCTGGTCCAGTGGAAAGGAAAAACGCGTCGAGAAGGAAGTTGAAGAAACATATGAAGAGAGAAAGAAAACAAGAGAGGCTCTTTCCCTAGGAGAGCAAATGCCAACTGTTCTGACTAGGAATGACAAGAAGAATCTTTCCTTTTCTCAGAAGGAAAAGAAGAAAAGGGATCTTGGTCAAGCAAGCAGGGGGAAAAATTATGTTGAAGAAGAGAAAAGGCTGTTGAGGGACAATGGAGTTTATTCTGGTTTCGATTCTTGA

Protein sequence

>MS.gene50546.t1

MPWSDDDDDSSSDSENDSETGAKKSKGKSTKQKKAPIDFEALKRYGYKGGPSVLKVPPPKEDDTKKDWSWSSGKEKRVEKEVEETYEERKKTREALSLGEQMPTVLTRNDKKNLSFSQKEKKKRDLGQASRGKNYVEEEKRLLRDNGVYSGFDS