AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51145


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51145.t1 MTR_7g080280 96.835 158 3 1 1 156 1 158 4.47e-112 317
MS.gene51145.t1 MTR_7g053107 60.526 114 44 1 39 152 36 148 4.63e-47 152
MS.gene51145.t1 MTR_2g010970 47.059 119 62 1 37 155 26 143 1.07e-35 122
MS.gene51145.t1 MTR_4g081560 48.718 117 59 1 39 155 29 144 3.89e-35 121
MS.gene51145.t1 MTR_4g091980 38.462 143 81 2 14 156 12 147 2.86e-29 106
MS.gene51145.t1 MTR_4g091970 35.065 154 94 1 3 156 1 148 5.46e-29 105
MS.gene51145.t1 MTR_4g091930 35.374 147 92 2 7 153 1 144 4.23e-27 100
MS.gene51145.t1 MTR_8g085580 40.870 115 67 1 38 152 29 142 2.92e-25 96.3
MS.gene51145.t1 MTR_4g064460 37.500 120 74 1 34 153 26 144 3.66e-25 95.9
MS.gene51145.t1 MTR_8g085590 41.379 116 65 3 39 153 31 144 4.33e-25 95.5
MS.gene51145.t1 MTR_2g010970 43.011 93 52 1 37 129 26 117 2.59e-23 90.1
MS.gene51145.t1 MTR_7g108775 29.710 138 90 3 16 152 11 142 1.77e-16 73.2
MS.gene51145.t1 MTR_7g108775 29.710 138 90 3 16 152 11 142 2.53e-16 73.6
MS.gene51145.t1 MTR_1g088250 29.060 117 78 2 38 152 23 136 2.12e-13 65.1
MS.gene51145.t1 MTR_6g086680 35.345 116 66 4 42 152 38 149 8.06e-12 60.8
MS.gene51145.t1 MTR_1g088260 30.556 108 69 3 47 152 4 107 9.01e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51145.t1 AT3G07470 50.685 146 64 1 17 154 1 146 7.44e-52 164
MS.gene51145.t1 AT3G07470 50.685 146 64 1 17 154 1 146 8.17e-52 164
MS.gene51145.t1 AT3G07470 50.685 146 64 1 17 154 1 146 1.47e-51 163
MS.gene51145.t1 AT3G07460 50.350 143 63 2 18 152 1 143 7.00e-48 154
MS.gene51145.t1 AT5G16380 53.623 138 64 0 16 153 8 145 2.17e-46 151
MS.gene51145.t1 AT3G07460 56.303 119 52 0 34 152 25 143 7.02e-46 152
MS.gene51145.t1 AT1G61667 53.719 121 56 0 34 154 17 137 2.14e-42 139
MS.gene51145.t1 AT1G61667 52.000 125 60 0 30 154 49 173 3.45e-42 140
MS.gene51145.t1 AT5G54530 50.000 118 58 1 38 155 28 144 4.21e-38 129
MS.gene51145.t1 AT5G19860 37.607 117 72 1 37 153 34 149 3.82e-22 88.6
MS.gene51145.t1 AT1G55265 38.843 121 71 3 39 158 54 172 1.67e-21 86.7
MS.gene51145.t1 AT4G02360 29.104 134 91 2 20 152 10 140 3.16e-19 80.1
MS.gene51145.t1 AT1G02813 31.884 138 85 3 16 152 7 136 4.04e-17 74.7
MS.gene51145.t1 AT4G02370 27.941 136 93 2 19 152 12 144 9.01e-17 74.3
MS.gene51145.t1 AT1G02816 27.692 130 89 2 25 152 18 144 1.90e-15 70.9
MS.gene51145.t1 AT2G03350 29.457 129 87 3 26 152 46 172 7.72e-12 61.2

Find 51 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTATGATCCTAATATTA+AGG 0.212709 7.3:+27239871 MS.gene51145:CDS
CAACCATATACGATGAATTA+AGG 0.222556 7.3:+27239726 MS.gene51145:CDS
TTCTCGTTCTTAGCGTTTCA+TGG 0.234067 7.3:+27239672 MS.gene51145:CDS
GTTCTTATGGTTTCCTGTTA+AGG 0.253897 7.3:+27239949 MS.gene51145:CDS
CTGCTCAGGAACTGTTCTTA+TGG 0.260029 7.3:+27239936 MS.gene51145:CDS
CATATCTATGTAGTTATTCT+TGG 0.277932 7.3:+27240404 MS.gene51145:intron
TTCTTATGGTTTCCTGTTAA+GGG 0.291646 7.3:+27239950 MS.gene51145:CDS
TGCCCTTAATTCATCGTATA+TGG 0.297917 7.3:-27239729 None:intergenic
CCAAGGGTACCCTTAATATT+AGG 0.319867 7.3:-27239881 None:intergenic
GGCAAGATCAAAGGATTATC+TGG 0.347833 7.3:+27239908 MS.gene51145:CDS
TGGAACCAGTGGTGAATTCC+AGG 0.358914 7.3:+27239805 MS.gene51145:CDS
ATTAAGGGTACCCTTGGTTA+TGG 0.369525 7.3:+27239887 MS.gene51145:CDS
TCAACACGAATGCCCTTAAC+AGG 0.369824 7.3:-27239962 None:intergenic
TCATACTTCGTTATTCCCTT+TGG 0.400855 7.3:-27239776 None:intergenic
ATATCTATGTAGTTATTCTT+GGG 0.428581 7.3:+27240405 MS.gene51145:intron
AACCATATACGATGAATTAA+GGG 0.433338 7.3:+27239727 MS.gene51145:CDS
CACTATGATCCTAATATTAA+GGG 0.436502 7.3:+27239872 MS.gene51145:CDS
GGTCTCATTCACTTTGATGT+TGG 0.437916 7.3:+27239998 MS.gene51145:CDS
CCTAATATTAAGGGTACCCT+TGG 0.447732 7.3:+27239881 MS.gene51145:CDS
CCAGTGGTGAATTCCAGGTT+TGG 0.463316 7.3:+27239810 MS.gene51145:CDS
TGGTTATGCACCTACGCAAA+CGG 0.492953 7.3:+27239692 MS.gene51145:CDS
CCCTTTGGTAGAAGCCCTAC+GGG 0.493865 7.3:-27239761 None:intergenic
TACGATGAATTAAGGGCAAA+GGG 0.498085 7.3:+27239734 MS.gene51145:CDS
AAAACCTCAGGGTTACAATC+AGG 0.505969 7.3:-27240064 None:intergenic
TGTCTAGTGACAACTGACTA+AGG 0.509334 7.3:-27240490 None:intergenic
CTCTCAATGGCTATGGCTAG+TGG 0.510825 7.3:+27239611 None:intergenic
CGTGTTGATCTACCTAGCTC+TGG 0.512045 7.3:+27239977 MS.gene51145:CDS
AGAAGAAGATGAAAATTCGA+TGG 0.513668 7.3:-27239647 None:intergenic
CTTGGTTATGGCAAGATCAA+AGG 0.516045 7.3:+27239899 MS.gene51145:CDS
TCCCTTTGGTAGAAGCCCTA+CGG 0.517563 7.3:-27239762 None:intergenic
TCCCGTAGGGCTTCTACCAA+AGG 0.520926 7.3:+27239760 MS.gene51145:CDS
CCCGTAGGGCTTCTACCAAA+GGG 0.524834 7.3:+27239761 MS.gene51145:CDS
ATAACGAAGTATGAGATCAA+TGG 0.526239 7.3:+27239785 MS.gene51145:CDS
ATTATCTGGTATGACTGCTC+AGG 0.527016 7.3:+27239922 MS.gene51145:CDS
GGGCAAAGGGTCTTCCCGTA+GGG 0.543039 7.3:+27239747 MS.gene51145:CDS
ATGGTTCTTTCATCCAAACC+TGG 0.549503 7.3:-27239823 None:intergenic
GATCTTATGTAAACAAGCCA+TGG 0.561139 7.3:+27240467 MS.gene51145:CDS
ATACGATGAATTAAGGGCAA+AGG 0.561325 7.3:+27239733 MS.gene51145:CDS
CCAAACCTGGAATTCACCAC+TGG 0.568465 7.3:-27239810 None:intergenic
TAGTGTTGATGATTAGAGTG+AGG 0.584750 7.3:+27240433 MS.gene51145:CDS
AAGTGAATGAGACCAGAGCT+AGG 0.587179 7.3:-27239989 None:intergenic
AGGGCAAAGGGTCTTCCCGT+AGG 0.591812 7.3:+27239746 MS.gene51145:CDS
TTTGATCTTGCCATAACCAA+GGG 0.597810 7.3:-27239897 None:intergenic
CTTTGATCTTGCCATAACCA+AGG 0.603621 7.3:-27239898 None:intergenic
TGAAAGAACCATGTAATGCA+AGG 0.604149 7.3:+27239834 MS.gene51145:CDS
GTGACAACTGACTAAGGCCA+TGG 0.613121 7.3:-27240484 None:intergenic
TAATGCAAGGTTTGAAAACG+AGG 0.621104 7.3:+27239847 MS.gene51145:CDS
ACCTCAGGGTTACAATCAGG+AGG 0.622444 7.3:-27240061 None:intergenic
TGAATTTGAACCGTTTGCGT+AGG 0.633813 7.3:-27239702 None:intergenic
TATGAGATCAATGGAACCAG+TGG 0.634964 7.3:+27239794 MS.gene51145:CDS
TCCTCCTGATTGTAACCCTG+AGG 0.653771 7.3:+27240060 MS.gene51145:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCATAATTTTTGTTATTT+GGG + chr7.3:27240319-27240338 MS.gene51145:intron 10.0%
!!! TATTTATTTTTTTCTCTTTT+TGG + chr7.3:27240108-27240127 MS.gene51145:intron 10.0%
!!! CATTCATAATTTTTGTTATT+TGG + chr7.3:27240318-27240337 MS.gene51145:intron 15.0%
!!! TGTGATTTTTTTTTTTTGTT+GGG + chr7.3:27240288-27240307 MS.gene51145:intron 15.0%
!!! TTGTGATTTTTTTTTTTTGT+TGG + chr7.3:27240287-27240306 MS.gene51145:intron 15.0%
!! ATAAATAACCAAATCAAGAA+TGG - chr7.3:27240095-27240114 None:intergenic 20.0%
!! ATATCTATGTAGTTATTCTT+GGG + chr7.3:27240405-27240424 MS.gene51145:intron 20.0%
!!! AAAGTTTTGATTTTTATGGT+TGG + chr7.3:27240343-27240362 MS.gene51145:intron 20.0%
!!! AAATTCATGTTTTTAAGAGT+TGG + chr7.3:27240221-27240240 MS.gene51145:intron 20.0%
!!! AAGTTTTGATTTTTATGGTT+GGG + chr7.3:27240344-27240363 MS.gene51145:intron 20.0%
!!! AATTCATGTTTTTAAGAGTT+GGG + chr7.3:27240222-27240241 MS.gene51145:intron 20.0%
!!! TTAAAGTTGTGATTTTTGTT+GGG + chr7.3:27240192-27240211 MS.gene51145:intron 20.0%
!!! TTTAAAGTTGTGATTTTTGT+TGG + chr7.3:27240191-27240210 MS.gene51145:intron 20.0%
!!! TTTTTTTTTTGTTGGGTATT+TGG + chr7.3:27240295-27240314 MS.gene51145:intron 20.0%
! AACCATATACGATGAATTAA+GGG + chr7.3:27239727-27239746 MS.gene51145:CDS 25.0%
! CACTATGATCCTAATATTAA+GGG + chr7.3:27239872-27239891 MS.gene51145:CDS 25.0%
! CATATCTATGTAGTTATTCT+TGG + chr7.3:27240404-27240423 MS.gene51145:intron 25.0%
! TCACTATGATCCTAATATTA+AGG + chr7.3:27239871-27239890 MS.gene51145:CDS 25.0%
!! TTTTTCATCCATTCTTGATT+TGG + chr7.3:27240084-27240103 MS.gene51145:intron 25.0%
!!! AGATTCATGTTTTTGTTATC+AGG + chr7.3:27240165-27240184 MS.gene51145:intron 25.0%
!!! GATTCATGTTTTTGTTATCA+GGG + chr7.3:27240166-27240185 MS.gene51145:intron 25.0%
!!! GGGTAAAGTTTTGATTTTTA+TGG + chr7.3:27240339-27240358 MS.gene51145:intron 25.0%
AGAAGAAGATGAAAATTCGA+TGG - chr7.3:27239650-27239669 None:intergenic 30.0%
ATAACGAAGTATGAGATCAA+TGG + chr7.3:27239785-27239804 MS.gene51145:CDS 30.0%
CAACCATATACGATGAATTA+AGG + chr7.3:27239726-27239745 MS.gene51145:CDS 30.0%
TGAAATTCATGTTGTGTAGT+TGG + chr7.3:27240135-27240154 MS.gene51145:intron 30.0%
TTCTTATGGTTTCCTGTTAA+GGG + chr7.3:27239950-27239969 MS.gene51145:CDS 30.0%
! TTTTCAAACCTTGCATTACA+TGG - chr7.3:27239845-27239864 None:intergenic 30.0%
!! ATTCAGGTTTTCTGTTATTC+GGG + chr7.3:27240253-27240272 MS.gene51145:intron 30.0%
!! GATTCAGGTTTTCTGTTATT+CGG + chr7.3:27240252-27240271 MS.gene51145:intron 30.0%
AGAATGGATGAAAAAACCTC+AGG - chr7.3:27240079-27240098 None:intergenic 35.0%
ATACGATGAATTAAGGGCAA+AGG + chr7.3:27239733-27239752 MS.gene51145:CDS 35.0%
GAATGGATGAAAAAACCTCA+GGG - chr7.3:27240078-27240097 None:intergenic 35.0%
GATCTTATGTAAACAAGCCA+TGG + chr7.3:27240467-27240486 MS.gene51145:CDS 35.0%
GTTCTTATGGTTTCCTGTTA+AGG + chr7.3:27239949-27239968 MS.gene51145:CDS 35.0%
TAATGCAAGGTTTGAAAACG+AGG + chr7.3:27239847-27239866 MS.gene51145:CDS 35.0%
TACGATGAATTAAGGGCAAA+GGG + chr7.3:27239734-27239753 MS.gene51145:CDS 35.0%
TCATACTTCGTTATTCCCTT+TGG - chr7.3:27239779-27239798 None:intergenic 35.0%
TGAAAGAACCATGTAATGCA+AGG + chr7.3:27239834-27239853 MS.gene51145:CDS 35.0%
TGCCCTTAATTCATCGTATA+TGG - chr7.3:27239732-27239751 None:intergenic 35.0%
TTTGATCTTGCCATAACCAA+GGG - chr7.3:27239900-27239919 None:intergenic 35.0%
!! TAGTGTTGATGATTAGAGTG+AGG + chr7.3:27240433-27240452 MS.gene51145:CDS 35.0%
AAAACCTCAGGGTTACAATC+AGG - chr7.3:27240067-27240086 None:intergenic 40.0%
ATGGTTCTTTCATCCAAACC+TGG - chr7.3:27239826-27239845 None:intergenic 40.0%
ATTATCTGGTATGACTGCTC+AGG + chr7.3:27239922-27239941 MS.gene51145:CDS 40.0%
CTTGGTTATGGCAAGATCAA+AGG + chr7.3:27239899-27239918 MS.gene51145:CDS 40.0%
CTTTGATCTTGCCATAACCA+AGG - chr7.3:27239901-27239920 None:intergenic 40.0%
GAGTTGGGTATATGAGATTC+AGG + chr7.3:27240237-27240256 MS.gene51145:intron 40.0%
GGCAAGATCAAAGGATTATC+TGG + chr7.3:27239908-27239927 MS.gene51145:CDS 40.0%
GGTCTCATTCACTTTGATGT+TGG + chr7.3:27239998-27240017 MS.gene51145:CDS 40.0%
TATGAGATCAATGGAACCAG+TGG + chr7.3:27239794-27239813 MS.gene51145:CDS 40.0%
TGAATTTGAACCGTTTGCGT+AGG - chr7.3:27239705-27239724 None:intergenic 40.0%
TTCTCGTTCTTAGCGTTTCA+TGG + chr7.3:27239672-27239691 MS.gene51145:CDS 40.0%
!! ATTAAGGGTACCCTTGGTTA+TGG + chr7.3:27239887-27239906 MS.gene51145:CDS 40.0%
!! CCAAGGGTACCCTTAATATT+AGG - chr7.3:27239884-27239903 None:intergenic 40.0%
!! CCTAATATTAAGGGTACCCT+TGG + chr7.3:27239881-27239900 MS.gene51145:CDS 40.0%
AAGTGAATGAGACCAGAGCT+AGG - chr7.3:27239992-27240011 None:intergenic 45.0%
CTGCTCAGGAACTGTTCTTA+TGG + chr7.3:27239936-27239955 MS.gene51145:CDS 45.0%
TCAACACGAATGCCCTTAAC+AGG - chr7.3:27239965-27239984 None:intergenic 45.0%
TGGTTATGCACCTACGCAAA+CGG + chr7.3:27239692-27239711 MS.gene51145:CDS 45.0%
ACCTCAGGGTTACAATCAGG+AGG - chr7.3:27240064-27240083 None:intergenic 50.0%
CCAAACCTGGAATTCACCAC+TGG - chr7.3:27239813-27239832 None:intergenic 50.0%
CCAGTGGTGAATTCCAGGTT+TGG + chr7.3:27239810-27239829 MS.gene51145:CDS 50.0%
GTGACAACTGACTAAGGCCA+TGG - chr7.3:27240487-27240506 None:intergenic 50.0%
TCCCTTTGGTAGAAGCCCTA+CGG - chr7.3:27239765-27239784 None:intergenic 50.0%
TCCTCCTGATTGTAACCCTG+AGG + chr7.3:27240060-27240079 MS.gene51145:CDS 50.0%
TGGAACCAGTGGTGAATTCC+AGG + chr7.3:27239805-27239824 MS.gene51145:CDS 50.0%
! CGTGTTGATCTACCTAGCTC+TGG + chr7.3:27239977-27239996 MS.gene51145:CDS 50.0%
CCCGTAGGGCTTCTACCAAA+GGG + chr7.3:27239761-27239780 MS.gene51145:CDS 55.0%
CCCTTTGGTAGAAGCCCTAC+GGG - chr7.3:27239764-27239783 None:intergenic 55.0%
TCCCGTAGGGCTTCTACCAA+AGG + chr7.3:27239760-27239779 MS.gene51145:CDS 55.0%
AGGGCAAAGGGTCTTCCCGT+AGG + chr7.3:27239746-27239765 MS.gene51145:CDS 60.0%
GGGCAAAGGGTCTTCCCGTA+GGG + chr7.3:27239747-27239766 MS.gene51145:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 27239617 27240509 27239617 ID=MS.gene51145
chr7.3 mRNA 27239617 27240509 27239617 ID=MS.gene51145.t1;Parent=MS.gene51145
chr7.3 exon 27239617 27240081 27239617 ID=MS.gene51145.t1.exon1;Parent=MS.gene51145.t1
chr7.3 CDS 27239617 27240081 27239617 ID=cds.MS.gene51145.t1;Parent=MS.gene51145.t1
chr7.3 exon 27240417 27240509 27240417 ID=MS.gene51145.t1.exon2;Parent=MS.gene51145.t1
chr7.3 CDS 27240417 27240509 27240417 ID=cds.MS.gene51145.t1;Parent=MS.gene51145.t1
Gene Sequence

>MS.gene51145

ATGGCTATGGCTAGTGGTGTTTCAAATTCTCCATCGAATTTTCATCTTCTTCTTCTTCTCGTTCTTAGCGTTTCATGGTTATGCACCTACGCAAACGGTTCAAATTCAACAACCATATACGATGAATTAAGGGCAAAGGGTCTTCCCGTAGGGCTTCTACCAAAGGGAATAACGAAGTATGAGATCAATGGAACCAGTGGTGAATTCCAGGTTTGGATGAAAGAACCATGTAATGCAAGGTTTGAAAACGAGGTTCACTATGATCCTAATATTAAGGGTACCCTTGGTTATGGCAAGATCAAAGGATTATCTGGTATGACTGCTCAGGAACTGTTCTTATGGTTTCCTGTTAAGGGCATTCGTGTTGATCTACCTAGCTCTGGTCTCATTCACTTTGATGTTGGTGTTGCTGATAAACAATTCTCTTTGTCTCTCTTTGAAGATCCTCCTGATTGTAACCCTGAGGTTTTTTCATCCATTCTTGATTTGGTTATTTATTTTTTTCTCTTTTTGGTATCTGAAATTCATGTTGTGTAGTTGGTTATATGAGATTCATGTTTTTGTTATCAGGGTATTTAAAGTTGTGATTTTTGTTGGGTGTATGAAATTCATGTTTTTAAGAGTTGGGTATATGAGATTCAGGTTTTCTGTTATTCGGGTAATTTTAAAGTTGTGATTTTTTTTTTTTGTTGGGTATTTGGCATTCATAATTTTTGTTATTTGGGTAAAGTTTTGATTTTTATGGTTGGGTATCTATGTATGATTGATGAATGATAGAAATTAAAATCATATCTATGTAGTTATTCTTGGGTGCAATAGTGTTGATGATTAGAGTGAGGTTTCAAGCTATGATCTTATGTAAACAAGCCATGGCCTTAGTCAGTTGTCACTAG

Protein sequence

>MS.gene51145.t1

MAMASGVSNSPSNFHLLLLLVLSVSWLCTYANGSNSTTIYDELRAKGLPVGLLPKGITKYEINGTSGEFQVWMKEPCNARFENEVHYDPNIKGTLGYGKIKGLSGMTAQELFLWFPVKGIRVDLPSSGLIHFDVGVADKQFSLSLFEDPPDCNPELFLGAIVLMIRVRFQAMILCKQAMALVSCH