Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54507.t1 | KEH19359.1 | 86.5 | 37 | 5 | 0 | 22 | 58 | 54 | 90 | 1.40E-07 | 65.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54507.t1 | A0A072TPG5 | 86.5 | 37 | 5 | 0 | 22 | 58 | 54 | 90 | 9.8e-08 | 65.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene54507 | 0.80242 | 5.66E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene54507 | 0.822637 | 2.13E-53 | -1.69E-46 |
| MS.gene050029 | MS.gene54507 | 0.802794 | 4.74E-49 | -1.69E-46 |
| MS.gene050430 | MS.gene54507 | 0.801114 | 1.05E-48 | -1.69E-46 |
| MS.gene050559 | MS.gene54507 | 0.809311 | 2.01E-50 | -1.69E-46 |
| MS.gene050561 | MS.gene54507 | 0.808293 | 3.33E-50 | -1.69E-46 |
| MS.gene051181 | MS.gene54507 | 0.824205 | 9.16E-54 | -1.69E-46 |
| MS.gene05125 | MS.gene54507 | 0.814737 | 1.32E-51 | -1.69E-46 |
| MS.gene051265 | MS.gene54507 | 0.807427 | 5.08E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene54507 | 0.818543 | 1.86E-52 | -1.69E-46 |
| MS.gene051492 | MS.gene54507 | 0.804617 | 1.98E-49 | -1.69E-46 |
| MS.gene051594 | MS.gene54507 | 0.814832 | 1.26E-51 | -1.69E-46 |
| MS.gene051957 | MS.gene54507 | 0.803438 | 3.49E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene54507 | 0.822883 | 1.87E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene54507 | 0.810035 | 1.41E-50 | -1.69E-46 |
| MS.gene052447 | MS.gene54507 | 0.836878 | 7.23E-57 | -1.69E-46 |
| MS.gene052519 | MS.gene54507 | 0.812651 | 3.81E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene54507 | 0.800757 | 1.24E-48 | -1.69E-46 |
| MS.gene052586 | MS.gene54507 | 0.802642 | 5.09E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene54507 | 0.8247 | 7.00E-54 | -1.69E-46 |
| MS.gene053166 | MS.gene54507 | 0.810959 | 8.89E-51 | -1.69E-46 |
| MS.gene053201 | MS.gene54507 | 0.806826 | 6.81E-50 | -1.69E-46 |
| MS.gene053966 | MS.gene54507 | 0.804502 | 2.09E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene54507 | 0.834725 | 2.54E-56 | -1.69E-46 |
| MS.gene05414 | MS.gene54507 | 0.81776 | 2.79E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene54507 | 0.834519 | 2.86E-56 | -1.69E-46 |
| MS.gene054291 | MS.gene54507 | -0.808422 | 3.12E-50 | -1.69E-46 |
| MS.gene054559 | MS.gene54507 | 0.803369 | 3.60E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene54507 | 0.808451 | 3.08E-50 | -1.69E-46 |
| MS.gene055227 | MS.gene54507 | 0.810176 | 1.31E-50 | -1.69E-46 |
| MS.gene055228 | MS.gene54507 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene54507 | 0.800652 | 1.30E-48 | -1.69E-46 |
| MS.gene055357 | MS.gene54507 | 0.813148 | 2.96E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene54507 | 0.818819 | 1.61E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene54507 | 0.813701 | 2.24E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene54507 | 0.806287 | 8.85E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene54507 | 0.801442 | 8.99E-49 | -1.69E-46 |
| MS.gene056859 | MS.gene54507 | 0.805304 | 1.42E-49 | -1.69E-46 |
| MS.gene057021 | MS.gene54507 | 0.949075 | 2.64E-107 | -1.69E-46 |
| MS.gene057026 | MS.gene54507 | 0.815592 | 8.54E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene54507 | 0.811888 | 5.59E-51 | -1.69E-46 |
| MS.gene057414 | MS.gene54507 | 0.816394 | 5.66E-52 | -1.69E-46 |
| MS.gene057444 | MS.gene54507 | 0.801875 | 7.33E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene54507 | 0.823122 | 1.64E-53 | -1.69E-46 |
| MS.gene058294 | MS.gene54507 | 0.846316 | 2.34E-59 | -1.69E-46 |
| MS.gene058625 | MS.gene54507 | 0.824507 | 7.77E-54 | -1.69E-46 |
| MS.gene058829 | MS.gene54507 | 0.804622 | 1.98E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene54507 | 0.801367 | 9.31E-49 | -1.69E-46 |
| MS.gene059015 | MS.gene54507 | 0.81482 | 1.27E-51 | -1.69E-46 |
| MS.gene059272 | MS.gene54507 | 0.820444 | 6.84E-53 | -1.69E-46 |
| MS.gene059584 | MS.gene54507 | 0.842377 | 2.68E-58 | -1.69E-46 |
| MS.gene060137 | MS.gene54507 | -0.810908 | 9.12E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene54507 | 0.804474 | 2.12E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54507.t1 | MTR_8g047190 | 84.211 | 38 | 6 | 0 | 22 | 59 | 54 | 91 | 1.24e-16 | 68.2 |
| MS.gene54507.t1 | MTR_0048s0080 | 49.091 | 55 | 28 | 0 | 25 | 79 | 281 | 335 | 1.26e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTCCAGTGATGAACTTT+TGG | 0.248742 | 7.4:-91287316 | MS.gene54507:CDS |
| TTTCCCAACGGTTTGCTTGC+TGG | 0.258707 | 7.4:+91286551 | None:intergenic |
| CAAGCAAACCGTTGGGAAAA+AGG | 0.271238 | 7.4:-91286547 | MS.gene54507:CDS |
| TGTGCGATCACCATTGAGTT+TGG | 0.378466 | 7.4:-91286500 | MS.gene54507:CDS |
| ATCCCTTCGACACTCTCAAC+TGG | 0.444586 | 7.4:+91286524 | None:intergenic |
| TGAAGATGCAATGCAGCTAA+AGG | 0.495947 | 7.4:-91287360 | None:intergenic |
| AACAGCCAAAAGTTCATCAC+TGG | 0.531595 | 7.4:+91287311 | None:intergenic |
| TACTCCAGCAAGCAAACCGT+TGG | 0.534254 | 7.4:-91286555 | MS.gene54507:CDS |
| TGCTACTAAAGAGTCGAAGA+TGG | 0.549976 | 7.4:-91286468 | MS.gene54507:CDS |
| ATCACCATTGAGTTTGGAGA+AGG | 0.557143 | 7.4:-91286494 | MS.gene54507:CDS |
| ACTCCAGCAAGCAAACCGTT+GGG | 0.557480 | 7.4:-91286554 | MS.gene54507:CDS |
| AGAGCCTTCTCCAAACTCAA+TGG | 0.564999 | 7.4:+91286490 | None:intergenic |
| TGATTCAAGCAACAAGGGAG+AGG | 0.570500 | 7.4:-91286594 | MS.gene54507:CDS |
| AGGCCAGTTGAGAGTGTCGA+AGG | 0.575521 | 7.4:-91286527 | MS.gene54507:CDS |
| AGGAGGTGATTCAAGCAACA+AGG | 0.594893 | 7.4:-91286600 | MS.gene54507:CDS |
| GCCAAAAGTTCATCACTGGA+AGG | 0.607816 | 7.4:+91287315 | None:intergenic |
| GGCCAGTTGAGAGTGTCGAA+GGG | 0.650645 | 7.4:-91286526 | MS.gene54507:CDS |
| GGAGGTGATTCAAGCAACAA+GGG | 0.650941 | 7.4:-91286599 | MS.gene54507:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAAAAAATTTCTTAAAAA+AGG | + | chr7.4:91286838-91286857 | None:intergenic | 10.0% |
| !!! | TAACATTGTTTTATTTTTGA+AGG | - | chr7.4:91286854-91286873 | MS.gene54507:intron | 15.0% |
| !!! | TTTATGCTTCATTTTAATTT+TGG | - | chr7.4:91287162-91287181 | MS.gene54507:intron | 15.0% |
| !! | AAACATTGAATATCATAAAC+AGG | + | chr7.4:91286572-91286591 | None:intergenic | 20.0% |
| !! | AAATCAATTGCTATGAAAAT+TGG | + | chr7.4:91286737-91286756 | None:intergenic | 20.0% |
| !! | AATTAAGAATTAAAGTGAGA+TGG | + | chr7.4:91287006-91287025 | None:intergenic | 20.0% |
| !! | CTATGAAAGTAAATATAGTT+TGG | - | chr7.4:91286927-91286946 | MS.gene54507:intron | 20.0% |
| !! | TATGAAAATTGGTATGATAA+CGG | + | chr7.4:91286726-91286745 | None:intergenic | 20.0% |
| !!! | AGGATTTTAACATTGTTATT+AGG | - | chr7.4:91286874-91286893 | MS.gene54507:intron | 20.0% |
| !!! | GTTTATGATATTCAATGTTT+TGG | - | chr7.4:91286572-91286591 | MS.gene54507:CDS | 20.0% |
| ! | ATGAAAATTGGTATGATAAC+GGG | + | chr7.4:91286725-91286744 | None:intergenic | 25.0% |
| !!! | ATGCTTCATTTTAATTTTGG+AGG | - | chr7.4:91287165-91287184 | MS.gene54507:intron | 25.0% |
| AGATACTGATAAGGAGAATA+TGG | + | chr7.4:91286601-91286620 | None:intergenic | 30.0% | |
| GACTAAAACAGATACTGATA+AGG | + | chr7.4:91286610-91286629 | None:intergenic | 30.0% | |
| ! | GCTGTTCTTTACTATTGTTT+TGG | - | chr7.4:91286687-91286706 | MS.gene54507:intron | 30.0% |
| ! | TGAAAATTGGTATGATAACG+GGG | + | chr7.4:91286724-91286743 | None:intergenic | 30.0% |
| AACTAAAATGCCATAGCAGA+TGG | - | chr7.4:91287062-91287081 | MS.gene54507:intron | 35.0% | |
| ATCTTTATGTTGCGTGGTTT+GGG | - | chr7.4:91286636-91286655 | MS.gene54507:intron | 35.0% | |
| CACGCAACATAAAGATAACA+GGG | + | chr7.4:91286632-91286651 | None:intergenic | 35.0% | |
| TAGTTGTTACAGCATCAGTT+AGG | + | chr7.4:91287047-91287066 | None:intergenic | 35.0% | |
| TATCTTTATGTTGCGTGGTT+TGG | - | chr7.4:91286635-91286654 | MS.gene54507:intron | 35.0% | |
| ! | AACAGCATTTTCTGACAATG+TGG | - | chr7.4:91286528-91286547 | MS.gene54507:CDS | 35.0% |
| ! | AGCTGTTTGGTATCATTTTC+TGG | + | chr7.4:91287089-91287108 | None:intergenic | 35.0% |
| ! | GCTGTTTGGTATCATTTTCT+GGG | + | chr7.4:91287088-91287107 | None:intergenic | 35.0% |
| ! | TGCATTGTTCCTTTTTGCTT+GGG | - | chr7.4:91286659-91286678 | MS.gene54507:intron | 35.0% |
| !! | ATGATAACGGGGAACAATAA+TGG | + | chr7.4:91286713-91286732 | None:intergenic | 35.0% |
| !! | CCAAACAGCTCTAAAAGATT+TGG | - | chr7.4:91287099-91287118 | MS.gene54507:intron | 35.0% |
| !! | TTAATTTTGGAGGCTCTTGA+AGG | - | chr7.4:91287175-91287194 | MS.gene54507:intron | 35.0% |
| !!! | CCAAATCTTTTAGAGCTGTT+TGG | + | chr7.4:91287102-91287121 | None:intergenic | 35.0% |
| AACAGCCAAAAGTTCATCAC+TGG | + | chr7.4:91286487-91286506 | None:intergenic | 40.0% | |
| ATCACCATTGAGTTTGGAGA+AGG | - | chr7.4:91287301-91287320 | MS.gene54507:intron | 40.0% | |
| CCACGCAACATAAAGATAAC+AGG | + | chr7.4:91286633-91286652 | None:intergenic | 40.0% | |
| CCTGTTATCTTTATGTTGCG+TGG | - | chr7.4:91286630-91286649 | MS.gene54507:intron | 40.0% | |
| TGCTACTAAAGAGTCGAAGA+TGG | - | chr7.4:91287327-91287346 | MS.gene54507:CDS | 40.0% | |
| TTGTTACAGCATCAGTTAGG+TGG | + | chr7.4:91287044-91287063 | None:intergenic | 40.0% | |
| ! | ACCTTCCAGTGATGAACTTT+TGG | - | chr7.4:91286479-91286498 | MS.gene54507:CDS | 40.0% |
| ! | GTGCATTGTTCCTTTTTGCT+TGG | - | chr7.4:91286658-91286677 | MS.gene54507:intron | 40.0% |
| ! | TTGTTCCTTTTTGCTTGGGT+TGG | - | chr7.4:91286663-91286682 | MS.gene54507:intron | 40.0% |
| AGAGCCTTCTCCAAACTCAA+TGG | + | chr7.4:91287308-91287327 | None:intergenic | 45.0% | |
| AGGAGGTGATTCAAGCAACA+AGG | - | chr7.4:91287195-91287214 | MS.gene54507:intron | 45.0% | |
| CAAGCAAACCGTTGGGAAAA+AGG | - | chr7.4:91287248-91287267 | MS.gene54507:intron | 45.0% | |
| GCCAAAAGTTCATCACTGGA+AGG | + | chr7.4:91286483-91286502 | None:intergenic | 45.0% | |
| GGAGGTGATTCAAGCAACAA+GGG | - | chr7.4:91287196-91287215 | MS.gene54507:intron | 45.0% | |
| TGATTCAAGCAACAAGGGAG+AGG | - | chr7.4:91287201-91287220 | MS.gene54507:intron | 45.0% | |
| TGTGCGATCACCATTGAGTT+TGG | - | chr7.4:91287295-91287314 | MS.gene54507:intron | 45.0% | |
| ! | ATTTTCTGGGCCATCTGCTA+TGG | + | chr7.4:91287075-91287094 | None:intergenic | 45.0% |
| !! | ATTTTGGAGGCTCTTGAAGG+AGG | - | chr7.4:91287178-91287197 | MS.gene54507:intron | 45.0% |
| !!! | TCAACTGGCCTTTTTCCCAA+CGG | + | chr7.4:91287259-91287278 | None:intergenic | 45.0% |
| ACTCCAGCAAGCAAACCGTT+GGG | - | chr7.4:91287241-91287260 | MS.gene54507:intron | 50.0% | |
| ATCCCTTCGACACTCTCAAC+TGG | + | chr7.4:91287274-91287293 | None:intergenic | 50.0% | |
| CAGCACCAACCCAAGCAAAA+AGG | + | chr7.4:91286671-91286690 | None:intergenic | 50.0% | |
| TACTCCAGCAAGCAAACCGT+TGG | - | chr7.4:91287240-91287259 | MS.gene54507:intron | 50.0% | |
| ! | TTTCCCAACGGTTTGCTTGC+TGG | + | chr7.4:91287247-91287266 | None:intergenic | 50.0% |
| !! | AGGCCAGTTGAGAGTGTCGA+AGG | - | chr7.4:91287268-91287287 | MS.gene54507:intron | 55.0% |
| !! | GGCCAGTTGAGAGTGTCGAA+GGG | - | chr7.4:91287269-91287288 | MS.gene54507:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 91286445 | 91287372 | 91286445 | ID=MS.gene54507 |
| chr7.4 | mRNA | 91286445 | 91287372 | 91286445 | ID=MS.gene54507.t1;Parent=MS.gene54507 |
| chr7.4 | exon | 91287307 | 91287372 | 91287307 | ID=MS.gene54507.t1.exon1;Parent=MS.gene54507.t1 |
| chr7.4 | CDS | 91287307 | 91287372 | 91287307 | ID=cds.MS.gene54507.t1;Parent=MS.gene54507.t1 |
| chr7.4 | exon | 91286445 | 91286630 | 91286445 | ID=MS.gene54507.t1.exon2;Parent=MS.gene54507.t1 |
| chr7.4 | CDS | 91286445 | 91286630 | 91286445 | ID=cds.MS.gene54507.t1;Parent=MS.gene54507.t1 |
| Gene Sequence |
| Protein sequence |
