Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57699.t1 | XP_013448012.1 | 97.6 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 2.30E-219 | 771.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57699.t1 | Q9LI89 | 48.5 | 388 | 184 | 5 | 4 | 375 | 34 | 421 | 1.0e-95 | 351.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57699.t1 | A0A072TX07 | 97.6 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 1.6e-219 | 771.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene57699 | -0.801735 | 7.82E-49 | -1.69E-46 |
| MS.gene049281 | MS.gene57699 | 0.848315 | 6.63E-60 | -1.69E-46 |
| MS.gene049320 | MS.gene57699 | -0.805954 | 1.04E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene57699 | -0.800232 | 1.59E-48 | -1.69E-46 |
| MS.gene050381 | MS.gene57699 | 0.822805 | 1.95E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene57699 | -0.818579 | 1.82E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene57699 | -0.825205 | 5.32E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene57699 | -0.820413 | 6.95E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene57699 | -0.81074 | 9.92E-51 | -1.69E-46 |
| MS.gene050675 | MS.gene57699 | -0.800542 | 1.37E-48 | -1.69E-46 |
| MS.gene050699 | MS.gene57699 | -0.82515 | 5.48E-54 | -1.69E-46 |
| MS.gene050883 | MS.gene57699 | 0.837245 | 5.82E-57 | -1.69E-46 |
| MS.gene050986 | MS.gene57699 | -0.801302 | 9.60E-49 | -1.69E-46 |
| MS.gene051127 | MS.gene57699 | 0.81887 | 1.56E-52 | -1.69E-46 |
| MS.gene051364 | MS.gene57699 | 0.834018 | 3.82E-56 | -1.69E-46 |
| MS.gene051366 | MS.gene57699 | 0.821333 | 4.27E-53 | -1.69E-46 |
| MS.gene052482 | MS.gene57699 | -0.800395 | 1.47E-48 | -1.69E-46 |
| MS.gene052486 | MS.gene57699 | -0.802416 | 5.67E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene57699 | -0.805014 | 1.64E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene57699 | 0.824868 | 6.39E-54 | -1.69E-46 |
| MS.gene053549 | MS.gene57699 | 0.812949 | 3.28E-51 | -1.69E-46 |
| MS.gene053826 | MS.gene57699 | 0.812731 | 3.66E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene57699 | 0.802019 | 6.84E-49 | -1.69E-46 |
| MS.gene054708 | MS.gene57699 | 0.802171 | 6.37E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene57699 | -0.822861 | 1.89E-53 | -1.69E-46 |
| MS.gene054954 | MS.gene57699 | -0.80358 | 3.26E-49 | -1.69E-46 |
| MS.gene054959 | MS.gene57699 | 0.837031 | 6.60E-57 | -1.69E-46 |
| MS.gene055154 | MS.gene57699 | 0.822862 | 1.89E-53 | -1.69E-46 |
| MS.gene055362 | MS.gene57699 | -0.805151 | 1.53E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene57699 | -0.828341 | 9.49E-55 | -1.69E-46 |
| MS.gene055577 | MS.gene57699 | -0.805823 | 1.11E-49 | -1.69E-46 |
| MS.gene056218 | MS.gene57699 | 0.806544 | 7.81E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene57699 | -0.818025 | 2.43E-52 | -1.69E-46 |
| MS.gene056692 | MS.gene57699 | -0.805348 | 1.39E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene57699 | 0.826481 | 2.65E-54 | -1.69E-46 |
| MS.gene056988 | MS.gene57699 | 0.804747 | 1.86E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene57699 | 0.814621 | 1.40E-51 | -1.69E-46 |
| MS.gene058068 | MS.gene57699 | 0.802697 | 4.96E-49 | -1.69E-46 |
| MS.gene058099 | MS.gene57699 | 0.812953 | 3.27E-51 | -1.69E-46 |
| MS.gene058111 | MS.gene57699 | 0.804314 | 2.29E-49 | -1.69E-46 |
| MS.gene058268 | MS.gene57699 | 0.806043 | 9.96E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene57699 | 0.802007 | 6.88E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene57699 | -0.80287 | 4.57E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene57699 | -0.805427 | 1.34E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene57699 | -0.815033 | 1.14E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene57699 | 0.814623 | 1.40E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene57699 | -0.817043 | 4.05E-52 | -1.69E-46 |
| MS.gene059023 | MS.gene57699 | -0.803502 | 3.38E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene57699 | -0.814274 | 1.68E-51 | -1.69E-46 |
| MS.gene059756 | MS.gene57699 | -0.813708 | 2.23E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene57699 | -0.803265 | 3.79E-49 | -1.69E-46 |
| MS.gene059953 | MS.gene57699 | 0.825953 | 3.54E-54 | -1.69E-46 |
| MS.gene059955 | MS.gene57699 | 0.808365 | 3.21E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene57699 | 0.819084 | 1.40E-52 | -1.69E-46 |
| MS.gene060778 | MS.gene57699 | -0.81011 | 1.36E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene57699 | -0.840279 | 9.57E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57699.t1 | MTR_7g029290 | 97.619 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 0.0 | 763 |
| MS.gene57699.t1 | MTR_6g023805 | 64.433 | 388 | 116 | 6 | 1 | 376 | 28 | 405 | 3.08e-168 | 476 |
| MS.gene57699.t1 | MTR_2g094093 | 39.420 | 345 | 201 | 6 | 32 | 374 | 24 | 362 | 2.02e-77 | 243 |
| MS.gene57699.t1 | MTR_4g108280 | 36.388 | 371 | 213 | 10 | 18 | 374 | 17 | 378 | 2.85e-71 | 228 |
| MS.gene57699.t1 | MTR_0345s0010 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 1.07e-70 | 228 |
| MS.gene57699.t1 | MTR_0105s0020 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 1.48e-70 | 228 |
| MS.gene57699.t1 | MTR_0105s0130 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 3.56e-70 | 226 |
| MS.gene57699.t1 | MTR_0105s0170 | 37.736 | 371 | 212 | 11 | 13 | 374 | 86 | 446 | 2.10e-69 | 225 |
| MS.gene57699.t1 | MTR_0496s0010 | 38.095 | 378 | 195 | 13 | 13 | 374 | 55 | 409 | 2.41e-69 | 224 |
| MS.gene57699.t1 | MTR_0105s0040 | 37.736 | 371 | 212 | 11 | 13 | 374 | 171 | 531 | 5.73e-69 | 226 |
| MS.gene57699.t1 | MTR_4g022260 | 37.037 | 378 | 205 | 13 | 13 | 374 | 55 | 415 | 1.96e-68 | 222 |
| MS.gene57699.t1 | MTR_8g468030 | 39.244 | 344 | 192 | 9 | 39 | 374 | 161 | 495 | 3.55e-68 | 223 |
| MS.gene57699.t1 | MTR_1330s0010 | 38.136 | 354 | 202 | 10 | 13 | 357 | 86 | 431 | 8.47e-68 | 221 |
| MS.gene57699.t1 | MTR_4g022400 | 35.890 | 365 | 217 | 9 | 18 | 374 | 57 | 412 | 1.13e-67 | 219 |
| MS.gene57699.t1 | MTR_5g039390 | 37.435 | 382 | 218 | 11 | 5 | 374 | 102 | 474 | 1.64e-67 | 221 |
| MS.gene57699.t1 | MTR_8g085600 | 27.303 | 304 | 200 | 10 | 5 | 299 | 55 | 346 | 4.28e-24 | 103 |
| MS.gene57699.t1 | MTR_4g114110 | 25.168 | 298 | 198 | 10 | 9 | 298 | 69 | 349 | 3.80e-19 | 89.0 |
| MS.gene57699.t1 | MTR_3g073640 | 25.652 | 230 | 156 | 5 | 31 | 254 | 46 | 266 | 1.21e-16 | 81.6 |
| MS.gene57699.t1 | MTR_7g095220 | 25.957 | 235 | 145 | 8 | 32 | 248 | 8 | 231 | 2.45e-15 | 76.6 |
| MS.gene57699.t1 | MTR_8g080370 | 24.471 | 331 | 213 | 12 | 14 | 325 | 33 | 345 | 9.47e-15 | 75.5 |
| MS.gene57699.t1 | MTR_3g085570 | 24.272 | 309 | 201 | 11 | 32 | 325 | 39 | 329 | 1.54e-13 | 71.6 |
| MS.gene57699.t1 | MTR_2g019230 | 23.776 | 286 | 197 | 9 | 32 | 304 | 56 | 333 | 1.40e-12 | 68.9 |
| MS.gene57699.t1 | MTR_2g008440 | 25.543 | 368 | 199 | 14 | 31 | 326 | 46 | 410 | 1.67e-12 | 68.9 |
| MS.gene57699.t1 | MTR_2g012790 | 29.091 | 165 | 101 | 3 | 138 | 292 | 109 | 267 | 2.99e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57699.t1 | AT3G27150 | 51.671 | 389 | 172 | 5 | 4 | 376 | 34 | 422 | 5.10e-133 | 387 |
| MS.gene57699.t1 | AT3G27150 | 51.671 | 389 | 172 | 5 | 4 | 376 | 34 | 422 | 5.10e-133 | 387 |
| MS.gene57699.t1 | AT5G40680 | 47.757 | 379 | 192 | 4 | 4 | 376 | 37 | 415 | 1.98e-119 | 352 |
| MS.gene57699.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57699.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57699.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57699.t1 | AT1G14330 | 39.650 | 343 | 192 | 9 | 39 | 374 | 105 | 439 | 1.55e-69 | 225 |
| MS.gene57699.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57699.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57699.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57699.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57699.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57699.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57699.t1 | AT1G26930 | 36.340 | 377 | 206 | 10 | 13 | 374 | 62 | 419 | 5.25e-64 | 210 |
| MS.gene57699.t1 | AT1G26930 | 30.769 | 377 | 175 | 12 | 13 | 374 | 62 | 367 | 1.46e-41 | 150 |
| MS.gene57699.t1 | AT1G55270 | 26.449 | 276 | 185 | 8 | 32 | 299 | 82 | 347 | 1.00e-20 | 93.6 |
| MS.gene57699.t1 | AT3G63220 | 23.810 | 315 | 195 | 13 | 32 | 328 | 15 | 302 | 2.75e-13 | 70.9 |
| MS.gene57699.t1 | AT3G63220 | 23.810 | 315 | 195 | 13 | 32 | 328 | 15 | 302 | 2.75e-13 | 70.9 |
| MS.gene57699.t1 | AT4G35120 | 28.241 | 216 | 139 | 6 | 31 | 234 | 29 | 240 | 3.21e-13 | 70.9 |
| MS.gene57699.t1 | AT3G63220 | 24.510 | 306 | 186 | 13 | 41 | 328 | 17 | 295 | 3.44e-13 | 70.5 |
| MS.gene57699.t1 | AT1G60570 | 25.574 | 305 | 197 | 10 | 10 | 299 | 2 | 291 | 2.22e-12 | 68.2 |
| MS.gene57699.t1 | AT1G55270 | 25.500 | 200 | 134 | 7 | 105 | 299 | 32 | 221 | 1.84e-11 | 65.1 |
| MS.gene57699.t1 | AT4G29370 | 29.500 | 200 | 118 | 8 | 17 | 204 | 14 | 202 | 2.36e-11 | 65.1 |
| MS.gene57699.t1 | AT1G22040 | 25.751 | 233 | 151 | 7 | 31 | 254 | 46 | 265 | 3.38e-11 | 65.1 |
| MS.gene57699.t1 | AT1G16250 | 22.074 | 376 | 258 | 10 | 32 | 378 | 12 | 381 | 4.17e-11 | 64.3 |
| MS.gene57699.t1 | AT1G16250 | 22.074 | 376 | 258 | 10 | 32 | 378 | 12 | 381 | 4.17e-11 | 64.3 |
| MS.gene57699.t1 | AT2G29830 | 21.446 | 401 | 270 | 15 | 1 | 375 | 1 | 382 | 4.49e-11 | 64.3 |
Find 75 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACACCTATAGCGAAATTT+TGG | 0.201938 | 7.4:+72142743 | MS.gene57699:CDS |
| AATTTGACGGACCTGTTATT+TGG | 0.224264 | 7.4:+72142604 | MS.gene57699:CDS |
| TCAGATTACAGCTTTGAATT+TGG | 0.229608 | 7.4:+72142531 | MS.gene57699:CDS |
| GGGTCAGAGTTATTTGTTTC+GGG | 0.243484 | 7.4:+72142576 | MS.gene57699:CDS |
| GACAAGAGATTCTATGTTAT+TGG | 0.250445 | 7.4:+72142858 | MS.gene57699:CDS |
| AGTTTCCAATGTTGCGATCT+TGG | 0.284283 | 7.4:-72142336 | None:intergenic |
| CTATTTGCTTCAGCTAGTTC+TGG | 0.294486 | 7.4:+72142687 | MS.gene57699:CDS |
| GCGGCGAGATCTACAAAATA+AGG | 0.302206 | 7.4:+72142397 | MS.gene57699:CDS |
| TTTCTCTTTGAATTCATATT+CGG | 0.325901 | 7.4:-72142813 | None:intergenic |
| AGCTGATGCACAAGGCGGTT+GGG | 0.354563 | 7.4:+72143118 | MS.gene57699:CDS |
| CAGAACTAGCTGAAGCAAAT+AGG | 0.366562 | 7.4:-72142686 | None:intergenic |
| GTTGAAAGACATTCCACTTT+CGG | 0.373291 | 7.4:+72142965 | MS.gene57699:CDS |
| TACAGCTTAATCATTTCATT+AGG | 0.374102 | 7.4:+72143288 | MS.gene57699:CDS |
| GGTGATGAGTTACTTGTTAT+CGG | 0.384004 | 7.4:+72143161 | MS.gene57699:CDS |
| CATTTCAATTTCTCACCCTC+AGG | 0.390524 | 7.4:-72143242 | None:intergenic |
| AGGGTCAGAGTTATTTGTTT+CGG | 0.390691 | 7.4:+72142575 | MS.gene57699:CDS |
| CTGTAATCTGATTGAATCCT+TGG | 0.399975 | 7.4:-72142519 | None:intergenic |
| GAATCATATCGCCAAATAAC+AGG | 0.403217 | 7.4:-72142615 | None:intergenic |
| ATTTGAAAGGGATCAATAAC+TGG | 0.407898 | 7.4:+72143069 | MS.gene57699:CDS |
| TGTTATTTGGCGATATGATT+CGG | 0.418939 | 7.4:+72142617 | MS.gene57699:CDS |
| AATTTCTCACCCTCAGGATC+AGG | 0.424534 | 7.4:-72143236 | None:intergenic |
| CGAAAGCAATTGGTGGGGTA+TGG | 0.427240 | 7.4:+72142467 | MS.gene57699:CDS |
| TACAAAATAAGGAAAGAGTT+AGG | 0.428564 | 7.4:+72142408 | MS.gene57699:CDS |
| TTCGCATAAAGAACGAGCTT+TGG | 0.431074 | 7.4:+72143196 | MS.gene57699:CDS |
| GGAATGTCTTTCAACATGTC+AGG | 0.452600 | 7.4:-72142957 | None:intergenic |
| TTTGAATTCATATTCGGTAA+AGG | 0.467260 | 7.4:-72142807 | None:intergenic |
| GCTGATGCACAAGGCGGTTG+GGG | 0.472323 | 7.4:+72143119 | MS.gene57699:CDS |
| CTGAGGGTGAGAAATTGAAA+TGG | 0.473233 | 7.4:+72143243 | MS.gene57699:CDS |
| TAGCTAGCGGCGAAAGCAAT+TGG | 0.476177 | 7.4:+72142457 | MS.gene57699:CDS |
| AGCTTCCTGCTTGATTTGAA+AGG | 0.476416 | 7.4:-72142495 | None:intergenic |
| CGAGCTAGAGACATCGATCT+TGG | 0.476661 | 7.4:+72142305 | MS.gene57699:CDS |
| ATTCTATGTTATTGGTGGAC+AGG | 0.479496 | 7.4:+72142866 | MS.gene57699:CDS |
| AGCTGAAAGTGTATTTGAAA+GGG | 0.490575 | 7.4:+72143057 | MS.gene57699:CDS |
| AATCAAGCAGGAAGCTTCCA+AGG | 0.494763 | 7.4:+72142502 | MS.gene57699:CDS |
| GAGCTGAAAGTGTATTTGAA+AGG | 0.497856 | 7.4:+72143056 | MS.gene57699:CDS |
| TAGGAATTGTGCTGTGATGA+TGG | 0.506085 | 7.4:+72143307 | MS.gene57699:CDS |
| GAGCTGATGCACAAGGCGGT+TGG | 0.506566 | 7.4:+72143117 | MS.gene57699:CDS |
| GATTTCCAAGATCGCAACAT+TGG | 0.509774 | 7.4:+72142331 | MS.gene57699:CDS |
| CCTTGTGCATCAGCTCTCAC+AGG | 0.510167 | 7.4:-72143110 | None:intergenic |
| AAATGGAAGCAAATTGATTG+TGG | 0.518481 | 7.4:+72143260 | MS.gene57699:CDS |
| GAAAGAAGAATCTTTACCTC+AGG | 0.525784 | 7.4:+72142254 | MS.gene57699:CDS |
| GTTTCGGGTAAAGAATTTGA+CGG | 0.527787 | 7.4:+72142591 | MS.gene57699:CDS |
| GGTGTCCAAAATTTCGCTAT+AGG | 0.532541 | 7.4:-72142748 | None:intergenic |
| TACTTGTTGTCCTGATCCTG+AGG | 0.533715 | 7.4:+72143226 | MS.gene57699:CDS |
| AAGAGATTCTATGTTATTGG+TGG | 0.534243 | 7.4:+72142861 | MS.gene57699:CDS |
| GATACTAATGAATGGTTCAA+AGG | 0.541527 | 7.4:+72142639 | MS.gene57699:CDS |
| ATGATTCGGATACTAATGAA+TGG | 0.555866 | 7.4:+72142631 | MS.gene57699:CDS |
| TGAAGAACCGTTGCCATCAG+AGG | 0.562265 | 7.4:-72142229 | None:intergenic |
| TCGACGAAGAAACAAACACT+TGG | 0.563005 | 7.4:+72142925 | MS.gene57699:CDS |
| GTCCGTTCATGATTAATCCT+AGG | 0.572154 | 7.4:+72142661 | MS.gene57699:CDS |
| GCAATTGGTGGGGTATGGAA+TGG | 0.578887 | 7.4:+72142472 | MS.gene57699:CDS |
| TAGCGGCGAAAGCAATTGGT+GGG | 0.579866 | 7.4:+72142461 | MS.gene57699:CDS |
| TTACCACCCTCTGATGGCAA+CGG | 0.580464 | 7.4:+72142222 | MS.gene57699:CDS |
| CGTTCTTTATGCGAAACAGA+AGG | 0.588464 | 7.4:-72143188 | None:intergenic |
| GAAGAACCGTTGCCATCAGA+GGG | 0.591725 | 7.4:-72142228 | None:intergenic |
| TATTAACAACAGCTATCAGA+GGG | 0.594157 | 7.4:-72143001 | None:intergenic |
| GGAGAGTGCGAATCCGAAAG+TGG | 0.596088 | 7.4:-72142978 | None:intergenic |
| ACTTGTTGTCCTGATCCTGA+GGG | 0.606724 | 7.4:+72143227 | MS.gene57699:CDS |
| GGTGTTGCATTTAAATCACT+TGG | 0.608539 | 7.4:+72143140 | MS.gene57699:CDS |
| GACTTGTTACCACCCTCTGA+TGG | 0.609485 | 7.4:+72142216 | MS.gene57699:CDS |
| CACCTAGGATTAATCATGAA+CGG | 0.612535 | 7.4:-72142663 | None:intergenic |
| AATGGCCTTTCAAATCAAGC+AGG | 0.615937 | 7.4:+72142490 | MS.gene57699:CDS |
| AGGAATTGTGCTGTGATGAT+GGG | 0.616810 | 7.4:+72143308 | MS.gene57699:CDS |
| CTAGCGGCGAAAGCAATTGG+TGG | 0.628757 | 7.4:+72142460 | MS.gene57699:CDS |
| TTATTAACAACAGCTATCAG+AGG | 0.633695 | 7.4:-72143002 | None:intergenic |
| CCTGTGAGAGCTGATGCACA+AGG | 0.635244 | 7.4:+72143110 | MS.gene57699:CDS |
| GCTGAAGCAAATAGGCACCT+AGG | 0.638194 | 7.4:-72142678 | None:intergenic |
| AGCGGCGAAAGCAATTGGTG+GGG | 0.663533 | 7.4:+72142462 | MS.gene57699:CDS |
| AGATTATAATCTGCATCCTG+AGG | 0.665618 | 7.4:-72142270 | None:intergenic |
| GAACCGTTGCCATCAGAGGG+TGG | 0.673262 | 7.4:-72142225 | None:intergenic |
| ATTAACAACAGCTATCAGAG+GGG | 0.676676 | 7.4:-72143000 | None:intergenic |
| TGGACAGGACGAGAATCGAA+AGG | 0.677851 | 7.4:+72142881 | MS.gene57699:CDS |
| TTTCGAATCATATTTCTCAG+CGG | 0.689567 | 7.4:-72142769 | None:intergenic |
| TTAACAACAGCTATCAGAGG+GGG | 0.692839 | 7.4:-72142999 | None:intergenic |
| GTGAGAGCTGATGCACAAGG+CGG | 0.771409 | 7.4:+72143113 | MS.gene57699:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATAACTGGAAAAAATT+AGG | + | chr7.4:72143080-72143099 | MS.gene57699:CDS | 20.0% |
| !! | TTTCTCTTTGAATTCATATT+CGG | - | chr7.4:72142816-72142835 | None:intergenic | 20.0% |
| ! | ATGATTCGAAAACAAAAACT+TGG | + | chr7.4:72142781-72142800 | MS.gene57699:CDS | 25.0% |
| ! | TACAAAATAAGGAAAGAGTT+AGG | + | chr7.4:72142408-72142427 | MS.gene57699:CDS | 25.0% |
| ! | TACAGCTTAATCATTTCATT+AGG | + | chr7.4:72143288-72143307 | MS.gene57699:CDS | 25.0% |
| ! | TTTGAATTCATATTCGGTAA+AGG | - | chr7.4:72142810-72142829 | None:intergenic | 25.0% |
| !! | ATAAAGAATCATTTTCAGCA+GGG | + | chr7.4:72142556-72142575 | MS.gene57699:CDS | 25.0% |
| !! | TCAAAGAGAAAATTTTGTTC+AGG | + | chr7.4:72142825-72142844 | MS.gene57699:CDS | 25.0% |
| AAATGGAAGCAAATTGATTG+TGG | + | chr7.4:72143260-72143279 | MS.gene57699:CDS | 30.0% | |
| AAGAGATTCTATGTTATTGG+TGG | + | chr7.4:72142861-72142880 | MS.gene57699:CDS | 30.0% | |
| ATTTGAAAGGGATCAATAAC+TGG | + | chr7.4:72143069-72143088 | MS.gene57699:CDS | 30.0% | |
| GACAAGAGATTCTATGTTAT+TGG | + | chr7.4:72142858-72142877 | MS.gene57699:CDS | 30.0% | |
| GATACTAATGAATGGTTCAA+AGG | + | chr7.4:72142639-72142658 | MS.gene57699:CDS | 30.0% | |
| TATTAACAACAGCTATCAGA+GGG | - | chr7.4:72143004-72143023 | None:intergenic | 30.0% | |
| TTATTAACAACAGCTATCAG+AGG | - | chr7.4:72143005-72143024 | None:intergenic | 30.0% | |
| TTTCGAATCATATTTCTCAG+CGG | - | chr7.4:72142772-72142791 | None:intergenic | 30.0% | |
| ! | AAACACCTATAGCGAAATTT+TGG | + | chr7.4:72142743-72142762 | MS.gene57699:CDS | 30.0% |
| ! | AGCTGAAAGTGTATTTGAAA+GGG | + | chr7.4:72143057-72143076 | MS.gene57699:CDS | 30.0% |
| ! | ATGATTCGGATACTAATGAA+TGG | + | chr7.4:72142631-72142650 | MS.gene57699:CDS | 30.0% |
| ! | ATTTTGTTCAGGATGTTACA+TGG | + | chr7.4:72142836-72142855 | MS.gene57699:CDS | 30.0% |
| ! | GATAAAGAATCATTTTCAGC+AGG | + | chr7.4:72142555-72142574 | MS.gene57699:CDS | 30.0% |
| ! | TGTTATTTGGCGATATGATT+CGG | + | chr7.4:72142617-72142636 | MS.gene57699:CDS | 30.0% |
| ! | TTTTTAACCCTGATGAAAAG+CGG | + | chr7.4:72142378-72142397 | MS.gene57699:CDS | 30.0% |
| !! | TCAGATTACAGCTTTGAATT+TGG | + | chr7.4:72142531-72142550 | MS.gene57699:CDS | 30.0% |
| AGATTATAATCTGCATCCTG+AGG | - | chr7.4:72142273-72142292 | None:intergenic | 35.0% | |
| AGGGTCAGAGTTATTTGTTT+CGG | + | chr7.4:72142575-72142594 | MS.gene57699:CDS | 35.0% | |
| ATTAACAACAGCTATCAGAG+GGG | - | chr7.4:72143003-72143022 | None:intergenic | 35.0% | |
| CACCTAGGATTAATCATGAA+CGG | - | chr7.4:72142666-72142685 | None:intergenic | 35.0% | |
| CTAGCTAACATAAAAACGGA+CGG | - | chr7.4:72142444-72142463 | None:intergenic | 35.0% | |
| CTGTAATCTGATTGAATCCT+TGG | - | chr7.4:72142522-72142541 | None:intergenic | 35.0% | |
| GAAAGAAGAATCTTTACCTC+AGG | + | chr7.4:72142254-72142273 | MS.gene57699:CDS | 35.0% | |
| GAATCATATCGCCAAATAAC+AGG | - | chr7.4:72142618-72142637 | None:intergenic | 35.0% | |
| GTTGAAAGACATTCCACTTT+CGG | + | chr7.4:72142965-72142984 | MS.gene57699:CDS | 35.0% | |
| ! | AATTTGACGGACCTGTTATT+TGG | + | chr7.4:72142604-72142623 | MS.gene57699:CDS | 35.0% |
| ! | ATTCTATGTTATTGGTGGAC+AGG | + | chr7.4:72142866-72142885 | MS.gene57699:CDS | 35.0% |
| ! | GAGCTGAAAGTGTATTTGAA+AGG | + | chr7.4:72143056-72143075 | MS.gene57699:CDS | 35.0% |
| ! | GGTGTTGCATTTAAATCACT+TGG | + | chr7.4:72143140-72143159 | MS.gene57699:CDS | 35.0% |
| ! | GTTTCGGGTAAAGAATTTGA+CGG | + | chr7.4:72142591-72142610 | MS.gene57699:CDS | 35.0% |
| !! | AATTTTGCTTTTGTTGCAGG+TGG | + | chr7.4:72142711-72142730 | MS.gene57699:CDS | 35.0% |
| !! | GGTAATTTTGCTTTTGTTGC+AGG | + | chr7.4:72142708-72142727 | MS.gene57699:CDS | 35.0% |
| !! | GGTGATGAGTTACTTGTTAT+CGG | + | chr7.4:72143161-72143180 | MS.gene57699:CDS | 35.0% |
| !! | TCCGTTTTTATGTTAGCTAG+CGG | + | chr7.4:72142444-72142463 | MS.gene57699:CDS | 35.0% |
| AATGGCCTTTCAAATCAAGC+AGG | + | chr7.4:72142490-72142509 | MS.gene57699:CDS | 40.0% | |
| AGGAATTGTGCTGTGATGAT+GGG | + | chr7.4:72143308-72143327 | MS.gene57699:CDS | 40.0% | |
| AGTTTCCAATGTTGCGATCT+TGG | - | chr7.4:72142339-72142358 | None:intergenic | 40.0% | |
| CAGAACTAGCTGAAGCAAAT+AGG | - | chr7.4:72142689-72142708 | None:intergenic | 40.0% | |
| CATTTCAATTTCTCACCCTC+AGG | - | chr7.4:72143245-72143264 | None:intergenic | 40.0% | |
| CGTTCTTTATGCGAAACAGA+AGG | - | chr7.4:72143191-72143210 | None:intergenic | 40.0% | |
| CTATTTGCTTCAGCTAGTTC+TGG | + | chr7.4:72142687-72142706 | MS.gene57699:CDS | 40.0% | |
| CTGAGGGTGAGAAATTGAAA+TGG | + | chr7.4:72143243-72143262 | MS.gene57699:CDS | 40.0% | |
| GATTTCCAAGATCGCAACAT+TGG | + | chr7.4:72142331-72142350 | MS.gene57699:CDS | 40.0% | |
| GCCGCTAGCTAACATAAAAA+CGG | - | chr7.4:72142448-72142467 | None:intergenic | 40.0% | |
| GGAATGTCTTTCAACATGTC+AGG | - | chr7.4:72142960-72142979 | None:intergenic | 40.0% | |
| GGGTCAGAGTTATTTGTTTC+GGG | + | chr7.4:72142576-72142595 | MS.gene57699:CDS | 40.0% | |
| GGTGTCCAAAATTTCGCTAT+AGG | - | chr7.4:72142751-72142770 | None:intergenic | 40.0% | |
| GTCCGTTCATGATTAATCCT+AGG | + | chr7.4:72142661-72142680 | MS.gene57699:CDS | 40.0% | |
| TAGGAATTGTGCTGTGATGA+TGG | + | chr7.4:72143307-72143326 | MS.gene57699:CDS | 40.0% | |
| TCGACGAAGAAACAAACACT+TGG | + | chr7.4:72142925-72142944 | MS.gene57699:CDS | 40.0% | |
| TTAACAACAGCTATCAGAGG+GGG | - | chr7.4:72143002-72143021 | None:intergenic | 40.0% | |
| TTCGCATAAAGAACGAGCTT+TGG | + | chr7.4:72143196-72143215 | MS.gene57699:CDS | 40.0% | |
| ! | AGCTTCCTGCTTGATTTGAA+AGG | - | chr7.4:72142498-72142517 | None:intergenic | 40.0% |
| AATCAAGCAGGAAGCTTCCA+AGG | + | chr7.4:72142502-72142521 | MS.gene57699:CDS | 45.0% | |
| AATTTCTCACCCTCAGGATC+AGG | - | chr7.4:72143239-72143258 | None:intergenic | 45.0% | |
| GCGGCGAGATCTACAAAATA+AGG | + | chr7.4:72142397-72142416 | MS.gene57699:CDS | 45.0% | |
| ! | TGCTTTTGTTGCAGGTGGTT+TGG | + | chr7.4:72142716-72142735 | MS.gene57699:CDS | 45.0% |
| !! | ACTTGTTGTCCTGATCCTGA+GGG | + | chr7.4:72143227-72143246 | MS.gene57699:CDS | 45.0% |
| !! | TACTTGTTGTCCTGATCCTG+AGG | + | chr7.4:72143226-72143245 | MS.gene57699:CDS | 45.0% |
| CGAAAGCAATTGGTGGGGTA+TGG | + | chr7.4:72142467-72142486 | MS.gene57699:CDS | 50.0% | |
| CGAGCTAGAGACATCGATCT+TGG | + | chr7.4:72142305-72142324 | MS.gene57699:CDS | 50.0% | |
| GAAGAACCGTTGCCATCAGA+GGG | - | chr7.4:72142231-72142250 | None:intergenic | 50.0% | |
| GCAATTGGTGGGGTATGGAA+TGG | + | chr7.4:72142472-72142491 | MS.gene57699:CDS | 50.0% | |
| GCTGAAGCAAATAGGCACCT+AGG | - | chr7.4:72142681-72142700 | None:intergenic | 50.0% | |
| TAGCGGCGAAAGCAATTGGT+GGG | + | chr7.4:72142461-72142480 | MS.gene57699:CDS | 50.0% | |
| TAGCTAGCGGCGAAAGCAAT+TGG | + | chr7.4:72142457-72142476 | MS.gene57699:CDS | 50.0% | |
| TGAAGAACCGTTGCCATCAG+AGG | - | chr7.4:72142232-72142251 | None:intergenic | 50.0% | |
| TGGACAGGACGAGAATCGAA+AGG | + | chr7.4:72142881-72142900 | MS.gene57699:CDS | 50.0% | |
| TTACCACCCTCTGATGGCAA+CGG | + | chr7.4:72142222-72142241 | MS.gene57699:CDS | 50.0% | |
| !! | GACTTGTTACCACCCTCTGA+TGG | + | chr7.4:72142216-72142235 | MS.gene57699:CDS | 50.0% |
| !!! | AGATCTCGCCGCTTTTCATC+AGG | - | chr7.4:72142389-72142408 | None:intergenic | 50.0% |
| !!! | GATCTCGCCGCTTTTCATCA+GGG | - | chr7.4:72142388-72142407 | None:intergenic | 50.0% |
| AGCGGCGAAAGCAATTGGTG+GGG | + | chr7.4:72142462-72142481 | MS.gene57699:CDS | 55.0% | |
| AGCTGATGCACAAGGCGGTT+GGG | + | chr7.4:72143118-72143137 | MS.gene57699:CDS | 55.0% | |
| CCTGTGAGAGCTGATGCACA+AGG | + | chr7.4:72143110-72143129 | MS.gene57699:CDS | 55.0% | |
| CCTTGTGCATCAGCTCTCAC+AGG | - | chr7.4:72143113-72143132 | None:intergenic | 55.0% | |
| CTAGCGGCGAAAGCAATTGG+TGG | + | chr7.4:72142460-72142479 | MS.gene57699:CDS | 55.0% | |
| GTGAGAGCTGATGCACAAGG+CGG | + | chr7.4:72143113-72143132 | MS.gene57699:CDS | 55.0% | |
| ! | GGAGAGTGCGAATCCGAAAG+TGG | - | chr7.4:72142981-72143000 | None:intergenic | 55.0% |
| GAACCGTTGCCATCAGAGGG+TGG | - | chr7.4:72142228-72142247 | None:intergenic | 60.0% | |
| GAGCTGATGCACAAGGCGGT+TGG | + | chr7.4:72143117-72143136 | MS.gene57699:CDS | 60.0% | |
| GCTGATGCACAAGGCGGTTG+GGG | + | chr7.4:72143119-72143138 | MS.gene57699:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 72142204 | 72143340 | 72142204 | ID=MS.gene57699 |
| chr7.4 | mRNA | 72142204 | 72143340 | 72142204 | ID=MS.gene57699.t1;Parent=MS.gene57699 |
| chr7.4 | exon | 72142204 | 72143340 | 72142204 | ID=MS.gene57699.t1.exon1;Parent=MS.gene57699.t1 |
| chr7.4 | CDS | 72142204 | 72143340 | 72142204 | ID=cds.MS.gene57699.t1;Parent=MS.gene57699.t1 |
| Gene Sequence |
| Protein sequence |
