AlfalfaGEDB | MS.gene60269

M. sativa cultivar XinJiangDaYe / MS.gene60269

KEGG: K21776
GO: -
PFAM: PF03638.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60269.t1	RHN76918.1	94.4	809	36	3	65	865	60	867	0.00E+00	1486.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60269.t1	Q9LUI3	31.8	547	253	20	310	843	254	693	6.0e-46	187.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60269.t1	A0A396JKR0	94.4	809	36	3	65	865	60	867	0.0e+00	1486.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene60269.t1	TF	CPP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049251	MS.gene60269	0.80108	1.07E-48	-1.69E-46
MS.gene049298	MS.gene60269	0.835036	2.12E-56	-1.69E-46
MS.gene049844	MS.gene60269	0.807008	6.24E-50	-1.69E-46
MS.gene050874	MS.gene60269	0.830217	3.33E-55	-1.69E-46
MS.gene050875	MS.gene60269	0.854164	1.48E-61	-1.69E-46
MS.gene05124	MS.gene60269	0.804464	2.13E-49	-1.69E-46
MS.gene051896	MS.gene60269	0.852182	5.46E-61	-1.69E-46
MS.gene051914	MS.gene60269	0.879097	1.65E-69	-1.69E-46
MS.gene052143	MS.gene60269	0.800515	1.39E-48	-1.69E-46
MS.gene052169	MS.gene60269	0.819006	1.46E-52	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60269.t1	MTR_1g012020	93.356	873	44	5	1	865	1	867	0.0	1603
MS.gene60269.t1	MTR_5g006530	48.404	188	94	3	473	659	240	425	2.27e-50	187
MS.gene60269.t1	MTR_5g006530	48.404	188	94	3	473	659	464	649	7.68e-50	189
MS.gene60269.t1	MTR_1g080640	62.500	96	34	1	422	517	5	98	2.57e-30	115
MS.gene60269.t1	MTR_8g095100	61.458	96	35	1	422	517	5	98	3.90e-30	115
MS.gene60269.t1	MTR_8g103320	41.040	173	90	6	469	635	52	218	1.89e-29	123
MS.gene60269.t1	MTR_1g101580	60.417	96	36	1	422	517	5	98	4.17e-29	112
MS.gene60269.t1	MTR_1g073270	60.417	96	36	1	422	517	5	98	5.80e-29	111
MS.gene60269.t1	MTR_2g049090	56.481	108	35	2	422	517	5	112	5.92e-29	112
MS.gene60269.t1	MTR_6g087590	47.727	132	60	3	496	624	128	253	1.48e-28	122

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60269.t1	AT3G22780	33.333	549	242	23	310	843	254	693	4.89e-61	220
MS.gene60269.t1	AT3G22760	39.812	319	139	10	318	636	77	342	1.90e-52	191
MS.gene60269.t1	AT3G22760	39.812	319	139	10	318	636	200	465	1.01e-51	192
MS.gene60269.t1	AT4G14770	38.636	308	138	8	317	624	226	482	2.84e-50	188
MS.gene60269.t1	AT4G14770	27.003	337	182	12	563	843	325	653	3.64e-14	77.0
MS.gene60269.t1	AT4G14770	38.636	308	138	8	317	624	225	481	2.85e-50	188
MS.gene60269.t1	AT4G14770	27.003	337	182	12	563	843	324	652	3.60e-14	77.0
MS.gene60269.t1	AT4G14770	38.636	308	138	8	317	624	245	501	4.25e-50	188
MS.gene60269.t1	AT4G14770	27.003	337	182	12	563	843	344	672	4.83e-14	76.6
MS.gene60269.t1	AT3G04850	32.857	350	167	10	296	621	271	576	3.73e-45	173

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Find 204 sgRNAs with CRISPR-Local

Find 392 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATTGATCATCCATAT+TGG	0.378531	1.1:-8747330	None:intergenic
AAAATCCTGCAACAATTGAA+GGG	0.574303	1.1:+8745796	MS.gene60269:CDS
AAAGAGGTTGCAATTGCAAA+AGG	0.504487	1.1:+8748088	MS.gene60269:CDS
AAATATTCATCGACAGATGC+TGG	0.465549	1.1:-8745588	None:intergenic
AAATCCTGCAACAATTGAAG+GGG	0.646037	1.1:+8745797	MS.gene60269:CDS
AAATGATGTATACTTGAACT+TGG	0.498024	1.1:-8744203	None:intergenic
AAATGCAACCCAATGCCTGG+TGG	0.691964	1.1:-8747173	None:intergenic
AAATGCACCTGATAAGAGGA+GGG	0.675925	1.1:+8747457	MS.gene60269:CDS
AACAGTCTTGGTGAAGCTCG+TGG	0.625856	1.1:+8745456	MS.gene60269:CDS
AACATGCTTCTAGCAATGAA+AGG	0.575833	1.1:+8749135	MS.gene60269:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATCCTGCAACAATTGAA+GGG	+	chr1.1:8745796-8745815	MS.gene60269:CDS	30.0%
AACTTAGATGATGAATCAGA+TGG	+	chr1.1:8749658-8749677	MS.gene60269:CDS	30.0%
AATGGAACAATATACTGATG+CGG	+	chr1.1:8745632-8745651	MS.gene60269:CDS	30.0%
ACCAAATACTAGTCCTTATA+AGG	+	chr1.1:8748419-8748438	MS.gene60269:intron	30.0%
ACTTGATAAGCAGAAAAATG+GGG	-	chr1.1:8746637-8746656	None:intergenic	30.0%
AGAATATTGTTCCAGAGATA+TGG	-	chr1.1:8744724-8744743	None:intergenic	30.0%
AGATTATCAGATGGTATACA+GGG	+	chr1.1:8747248-8747267	MS.gene60269:CDS	30.0%
ATAATTGTTACAGTCAATGC+AGG	+	chr1.1:8747007-8747026	MS.gene60269:intron	30.0%
ATATTAGATTCCTCCAACAA+AGG	-	chr1.1:8745747-8745766	None:intergenic	30.0%
ATCGTCAATAACAAAAGACA+CGG	-	chr1.1:8745344-8745363	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	8744096	8749963	8744096	ID=MS.gene60269
chr1.1	mRNA	8744096	8749963	8744096	ID=MS.gene60269.t1;Parent=MS.gene60269
chr1.1	exon	8744096	8744212	8744096	ID=MS.gene60269.t1.exon1;Parent=MS.gene60269.t1
chr1.1	CDS	8744096	8744212	8744096	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8745161	8745318	8745161	ID=MS.gene60269.t1.exon2;Parent=MS.gene60269.t1
chr1.1	CDS	8745161	8745318	8745161	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8745401	8745923	8745401	ID=MS.gene60269.t1.exon3;Parent=MS.gene60269.t1
chr1.1	CDS	8745401	8745923	8745401	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8746058	8746207	8746058	ID=MS.gene60269.t1.exon4;Parent=MS.gene60269.t1
chr1.1	CDS	8746058	8746207	8746058	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8747029	8747531	8747029	ID=MS.gene60269.t1.exon5;Parent=MS.gene60269.t1
chr1.1	CDS	8747029	8747531	8747029	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8747630	8747701	8747630	ID=MS.gene60269.t1.exon6;Parent=MS.gene60269.t1
chr1.1	CDS	8747630	8747701	8747630	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8747784	8747967	8747784	ID=MS.gene60269.t1.exon7;Parent=MS.gene60269.t1
chr1.1	CDS	8747784	8747967	8747784	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8748048	8748146	8748048	ID=MS.gene60269.t1.exon8;Parent=MS.gene60269.t1
chr1.1	CDS	8748048	8748146	8748048	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8748240	8748306	8748240	ID=MS.gene60269.t1.exon9;Parent=MS.gene60269.t1
chr1.1	CDS	8748240	8748306	8748240	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8749120	8749288	8749120	ID=MS.gene60269.t1.exon10;Parent=MS.gene60269.t1
chr1.1	CDS	8749120	8749288	8749120	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1
chr1.1	exon	8749408	8749963	8749408	ID=MS.gene60269.t1.exon11;Parent=MS.gene60269.t1
chr1.1	CDS	8749408	8749963	8749408	ID=cds.MS.gene60269.t1;Parent=MS.gene60269.t1

Gene Sequence

>MS.gene60269

ATGGATTCTCCAGAAGCTTCTCAGATTAAGAACATTTCTTCTTCTTCCTCTCTCTCCTCAACTCTCAATGCCGCCACCGCCATCACCAATCCTTCTGATGAATCCCCCCAAGTTCAAGTATACATCATTTCATTTTCATACCAATTAATTAATTATGCTACCCCCCTTTGATTGGGGTTCTTGACACGATTTTCGAGTTTAATTCTTCTTCTTTACGATTATTATGTTATGGAACTTCATCATGATATGATACTATCAATGTTAACTCTGTCTGTCTTTATGTTTGGAATCTAATCTAATGAATTTATACCATTTTATTTCATTTATTGTGTTAGACATGTTTGGAATTACATTTGTTTTCGATGCCTACTTTTTGTTTCATTCCATTCATCAAACAACATTGTCTCTTCTTTTTTTGCATCAAAATGAATATAAGGTTATCAATTAGATCGGTTGATTCTTAGATCCTTAGACCGGATACTGAGTTTTCACAAGAAAATAAAAAAAGGTTATCTATTAAGTAAGTTTTAATCAATGTTGTCCGTCGTGGATTGCGAAAAATAGCATTTTGTTCAAATTCTACTATGTTACGGCGCCGTTATTTGACATTGCATTGTACTGAATACCATATCTCTGGAACAATATTCTGCTACACTATAGCCACTATTTGACAGTGCTGTGTTCATGTACTTAAGAGGTTTTACGCACTTGTGCGTTGTGGTGCCGCACTTTGATAAACAGTGAGAGACTTGTTTGGAATTATATTTGTTTGATTGGATAAAATTCACTAGACTTGCTTTTGGTCTATCCTCTCTTATGTTAATTAACCCACACCGTCTCATTTTTGCATTAAAATGCTTATCAATTAGGCGTATGTGCATTGTCGGACTGGTATCTTGTGTTGATTGTGTTTCTAGAACCGTCTTTTTTGTTTAGAGATGTCTAGACTAGAGAGTTGTGTATTATTTTTGTTGACTTGTGTAGTATTTTTCAGACTCTTTCATTTCATTTCTAGTATCACGAAAAAGGAATGAAATTTACATCTTGTCGTCTTCTTCATTTCAGGAATCTCCCTTTTTGAGATTTGTCAACACTCTGTCTCCAATAAAGTCTGTCAAGGCTTCTCATGCGACACATGGGTTTCTCGGACTCAATTCTCCGCCTCTCGTATTCAAGTCTCCGCGTATCAGTGCTCCCCACCGTGAAACACAATACTCGGAAAGGTTTGTGACTTCAATTTTGTGTCCGTGTCTTTTGTTATTGACGATGCGCTTGCTGTTTATTGACAAAATTTGTATGTATCAGGCTTGAAGGTACCCATTTATCAGACACAGAAACATCTCAATGTAACGTAGGAGACAACAGTCTTGGTGAAGCTCGTGGTGATCTGAAGAAATTGAATTCTCAACAGCCACTGCCAGAGGTGTTTATAACAGTTGCTCAGAAGGATTTTGACCTCAAGAATGACACGAATACCCAGAGCAGCAGTCCTCCAGCATCTGTCGATGAATATTTGGCTGATCCCGTGGATGATCAAATGGAACAATATACTGATGCGGTTGAGTCATCATCACTAACTGAGTCGGAGAAATTTATATTAAAATTTGATAGGAGTGACGGTCCTAGTAACATAGCAGAAGAACTTTTGCCTTTGTTGGAGGAATCTAATATGGTTCATCAAGAAAGGACAACATATGTTGAAAATCCTGCAACAATTGAAGGGGAGAAAAATGGTGCTGAATTGGTTTCTCAAGAGCACATAAACTTAGGCTCGAGTTCAGGTGCAGATGTTTTTGATAAACAATATTGCCATGATTCACTTCCCCAGGTAACTAAGCTCTTGCTGTCTATACTCTATATTTATAATGCTGATATAGGGACGTCAGTTTTCATATATATGATTAGTGTGAGCTAGATGTCCTCTAGTCTCTTTTGATTGATCTATAAAATTTCAAGCTTCAGTGTGCAGGAAATGATCAACAGCATCAAAGTGACTGTGCACCTCAATTGATGCCTGATCCTATCCAGGCTGTTAAGGAATTTGAAAATTGCAGTGAGATGGTGTCATCCTCACAAGTAAATTCAGAGAATATCCCACAAGATGGTTCTGAGGTAATTGATTTTTTTTCTTTCAGGTTTATTGCATTCCTCTGATGAATATATGAACTTTTGTTTAGATTTTGCTGTCTTTTCATGCTTTCCCTCCTCTCTGAATGATTTTGTTTATGTCTGTGTTCTTTTAGTCTTTAACACTAAATACTAGTCTCTTTAGAGGTTAATGACTCCAGGCTTGAATACACTTTTAGTTTTTTTAATCATTGGCATCTATTATCTCCAATTAAAGAAGAGCACAGTACATGTATTTTGTTTGCACTTGGCACATTCAATCTTATAAATCTAATAATGCTTCATTGATCTAGTTTGTTTATGGCCATTTGCATGCATCATGGCAGGATTTTTTTAACAAAATTAGCAGAAGTGGTATACTGCATATTTCTGCATAGGTGCTGTAATCAGATTTCTCTAACTGAAAAATCCCCCCATTTTTCTGCTTATCAAGTTTTGCTTTGTTTGGAGGTTTCAAAATTGTGGATCTCTGATAAATACAGTTTTTTCAACAAGATTGCTAATAAAATGCGAGTCCTGATATTACATCATGGACTCAAGGTTAAGGGCATCTCTTAGAATAAGGAAGTCAGTCTTCTGTTTATGAATATATAGCATGAAATATCTTTTGAACAAAGAAAAGTCAGCAATAATTTATTGGCTACAAGGTTTTAGTCATGTAGGATGAGACTACAGTGGTGTAATGGCATTATCTACTTGGCAATCATACACAGAGTTGCATTTTGATTATCAGGAACTTGCATTTTATTGTTCAATTAAATGAAGATTTTCTTTCTGCAATATAATAATTGTTACAGTCAATGCAGGCTAGCCTAAAGTATCATGGCATCCGTAGACGCTGCCTACAATTTGGGGAAGCTGCTTCCGTTGTTCTTGGAAGTAATAAGTCTCATGTGAAATTGAATACAACTTCAAGCAACGCAAAAATGGTTTCTGTGACAGTTTCCAAGCCACCAGGCATTGGGTTGCATTTAAATAGCATTATAAATGCAATGCCACCTAGTTGTGCTTCAACTACAGGTGTGAGATTATCAGATGGTATACAGGGGTCCAAATCTTCTATAAGTTTGCATATAGTGGAAAACGTGGCAAGATCCTCAACTCCGTCCAATATGGATGATCAATCTTTTATTGATACTAAGAATGAGAGCCATGAAACTGATGCTTCAGTAGTTGCAGATTCTTTCATTTCCGAGTCTCCTATCTTGACAGAATCCATCGACCTATATCCGGCAAATGCACCTGATAAGAGGAGGGTACGCCCCACAGATGCTGAGGAATTCAACCATCCTAGCACCTCCAGGAAAAAGTTAGTTATCATGATCTTTCTCATAGGTCATTATTTTTATTCTTTACCTGTTTGCTAGTTTTACCAAATATTACCAAATATTGCATTGCTCTATATAGGAAGAAAACAAGTACTGATGATGCCAATGGACCTAAGACGTGCAACTGTAAGAAGAGTAAATGTTTGAAACTGTGAGTAGATTTTAAATATGTTTTGATACCTTACTTTCTATGATATGCAATATCTATGTATTTTGATTTGAGTACTTTGCAGTTACTGTGATTGTTTTGGTGCTGGAATCTTCTGTGGCGACGGTTGTGCTTGCGAAGGCTGTGGTAACAGAGTAGAATTTCAGGATAAAGTTGTTGAAACAAAACAGCAAATTGAATCCCGTAATCCACAAGCATTTGCTCCCAAGATTGTACCATGTGCAGCTGATGTTCCACTGAATAATATGGTATAAGATCTTCATATATCACTGTTGTTCAACCTGCTAGCCTTAAAAAATTAGGTGATATTTTGATGTGACCTTTGCAGGAAGATGTGAATATGACAACACCGGCATCAGCTAGGCACAAAAGAGGTTGCAATTGCAAAAGGTCAAAGTGTACAAAAAAATACTGTGAATGTTTTCAGGTTGTGTATTTTATCCATATTTCTATATGCTCTAAATATTTTTACTGTGTCTGCTAATATATTTTTTATTTTATTTTAAATATTTGTCAAAAGGCTAACGTTGGATGCTCGACTGGATGTCGATGTGATGGATGTATGAATGTCTTTGGCAAGAGAGAAGGTTAGAGCTCATAGTATGAATAGCTATTTTAAATACTTAATGCACTACACAGTTTAACTTGTTGTCTGGTCAGTATAAATGTTATGGATTGATGTTGGGAGGTTTTGATGTTACCAAATACTAGTCCTTATAAGGAAAATTGTTGAGGCCAGCCCAGCACAACTTTTAAGTTTTTTAATGATGTGTAGATTTGAATCCAGAACCTCAACTCAAACCCTACCCACCACCAAGTCCAGGTAGAATGCTAGAGATGGTCATATTTGCCATACAAATGACACCCCTTTGCTTTATGCTGCCAGTAATAATCTCTGCGGTGCACTTTTGGAAGTGGTGGGTTTGTTTTAAAAGAAGTATTAGGTCTCGCGAACTTATGAATTGAATCAATTACGATTAAATAACTTTTTAGTGTTTCTAATGTACACTATTCCCAATTTAGTTTCTAAAATTTCTAGTTGATTTCAATCCCTGAAATACAATAACATAATTTTTGGTACTTCTTTTGGTTTGAGTTCTTGTAACTTTGTCCACATTAAAATGAATACATATGGTCCACATGTTATAGGAATTTATTTCAGCATGGACAAATATTTTGAAAAACTAAATTAAAAGAATGAAAATCAAAAGCAATTTTTCTTAAATTTTAGGGGTTCAAACTGGCTAGAATTTCTTGAGAGACTAAACTGAAAGTTGGTGTGAATTACGGAGACTAGAATTATTTTAGCTTTTAAAGTATAGTTGACATCCACATGAGGGGAGAGTGGGTGCAGATCATTAAATGTTTCTGTTAATGTATTACACATTTTTTAAATGCAGATTTTGTGGCAATGGAACATGCTTCTAGCAATGAAAGGGAGAGCAACATTGTTGAAGAAGGGTTGGATGACAAACTGCACAAAAGACAGAAAATTGTGACAATCAGGACAGACTTGTTACGACCAGCAAATCACCTCTCACCTGTAACACCATTGTTGCAATGCTCTGAGTAAGATTGCTTACATTACAAGTTTTTCTATGTTGTATAAATAAGAAGTTATATTCTGAGTATTTAATTATTTTGACGTCCTTTCATTTGTGCCGATTGGCCTTTTTGCATTTGTCCAGCCAAGGCAAGCAGGGTGCAAAGTCTAGACATACTTCTGCAAATTTGACCAAATCTTCTAAAAAACCACGCAGCAGTCTGGCACATTCGAAGTCGAATGATTCTCAAAAGAATGCACCTACACGTTCTTCATCTACAGAAAATGAGTGGATAGATCTTCCACCATACCAACTCTCCAATAGATGCGGTATTCGCCAGTTATCTGGGGGCTCTCTTCGTTGGCGCGGTAGTTCACCGATTACCCCAAGCGCTAACTTAGATGATGAATCAGATGGTAAGCTCTTTGACATTATAGAAGATGAAACACCTGATGTATTGAAGGAAGCTTCAACTCCCATCAAGTCTGTGAAAGCAAATTCTCCAATCCAAAAGAGAGTTTCTCCTCCACAGAGCCATCTACTTCGGATTGGACCAAGCTCCTCAGGTGGCTTGAAAAGTGGCAGGAAATTTATATTGCAATCTGTTCCTTCATTCCCGCCGTTGACCCCTTGCGCTGATTCTAAAGTCCTTTGCAATGGCAATGAGGATTCGGGTAACAGCACAGATAAAGTGACATAA

Protein sequence

>MS.gene60269.t1

MDSPEASQIKNISSSSSLSSTLNAATAITNPSDESPQVQESPFLRFVNTLSPIKSVKASHATHGFLGLNSPPLVFKSPRISAPHRETQYSERLEGTHLSDTETSQCNVGDNSLGEARGDLKKLNSQQPLPEVFITVAQKDFDLKNDTNTQSSSPPASVDEYLADPVDDQMEQYTDAVESSSLTESEKFILKFDRSDGPSNIAEELLPLLEESNMVHQERTTYVENPATIEGEKNGAELVSQEHINLGSSSGADVFDKQYCHDSLPQCAGNDQQHQSDCAPQLMPDPIQAVKEFENCSEMVSSSQVNSENIPQDGSEASLKYHGIRRRCLQFGEAASVVLGSNKSHVKLNTTSSNAKMVSVTVSKPPGIGLHLNSIINAMPPSCASTTGVRLSDGIQGSKSSISLHIVENVARSSTPSNMDDQSFIDTKNESHETDASVVADSFISESPILTESIDLYPANAPDKRRVRPTDAEEFNHPSTSRKKKKTSTDDANGPKTCNCKKSKCLKLYCDCFGAGIFCGDGCACEGCGNRVEFQDKVVETKQQIESRNPQAFAPKIVPCAADVPLNNMEDVNMTTPASARHKRGCNCKRSKCTKKYCECFQANVGCSTGCRCDGCMNVFGKREDFVAMEHASSNERESNIVEEGLDDKLHKRQKIVTIRTDLLRPANHLSPVTPLLQCSDQGKQGAKSRHTSANLTKSSKKPRSSLAHSKSNDSQKNAPTRSSSTENEWIDLPPYQLSNRCGIRQLSGGSLRWRGSSPITPSANLDDESDGKLFDIIEDETPDVLKEASTPIKSVKANSPIQKRVSPPQSHLLRIGPSSSGGLKSGRKFILQSVPSFPPLTPCADSKVLCNGNEDSGNSTDKVT