Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63902.t1 | XP_003617746.2 | 82.9 | 146 | 12 | 2 | 44 | 176 | 212 | 357 | 1.60E-53 | 219.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63902.t1 | G7K4I9 | 82.9 | 146 | 12 | 2 | 44 | 176 | 212 | 357 | 1.1e-53 | 219.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049341 | MS.gene63902 | 0.825861 | 3.72E-54 | -1.69E-46 |
| MS.gene049382 | MS.gene63902 | 0.824531 | 7.67E-54 | -1.69E-46 |
| MS.gene049703 | MS.gene63902 | -0.804187 | 2.44E-49 | -1.69E-46 |
| MS.gene049756 | MS.gene63902 | 0.800651 | 1.30E-48 | -1.69E-46 |
| MS.gene050277 | MS.gene63902 | 0.834227 | 3.39E-56 | -1.69E-46 |
| MS.gene050586 | MS.gene63902 | 0.80305 | 4.19E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene63902 | 0.845446 | 4.04E-59 | -1.69E-46 |
| MS.gene050808 | MS.gene63902 | 0.808355 | 3.23E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene63902 | 0.82625 | 3.01E-54 | -1.69E-46 |
| MS.gene050838 | MS.gene63902 | 0.808341 | 3.25E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene63902 | 0.821513 | 3.88E-53 | -1.69E-46 |
| MS.gene051102 | MS.gene63902 | 0.802807 | 4.71E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene63902 | 0.812621 | 3.87E-51 | -1.69E-46 |
| MS.gene051245 | MS.gene63902 | 0.813732 | 2.21E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene63902 | 0.822978 | 1.77E-53 | -1.69E-46 |
| MS.gene051626 | MS.gene63902 | 0.801884 | 7.29E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene63902 | 0.826541 | 2.56E-54 | -1.69E-46 |
| MS.gene052133 | MS.gene63902 | 0.825957 | 3.53E-54 | -1.69E-46 |
| MS.gene052286 | MS.gene63902 | 0.808435 | 3.10E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene63902 | 0.825752 | 3.95E-54 | -1.69E-46 |
| MS.gene052376 | MS.gene63902 | 0.818389 | 2.01E-52 | -1.69E-46 |
| MS.gene052397 | MS.gene63902 | 0.822895 | 1.85E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene63902 | 0.833733 | 4.50E-56 | -1.69E-46 |
| MS.gene052856 | MS.gene63902 | 0.810626 | 1.05E-50 | -1.69E-46 |
| MS.gene052858 | MS.gene63902 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene052860 | MS.gene63902 | 0.813741 | 2.19E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene63902 | 0.802027 | 6.82E-49 | -1.69E-46 |
| MS.gene053109 | MS.gene63902 | 0.811156 | 8.06E-51 | -1.69E-46 |
| MS.gene053437 | MS.gene63902 | 0.831533 | 1.58E-55 | -1.69E-46 |
| MS.gene054379 | MS.gene63902 | 0.802314 | 5.95E-49 | -1.69E-46 |
| MS.gene05462 | MS.gene63902 | 0.815804 | 7.66E-52 | -1.69E-46 |
| MS.gene055174 | MS.gene63902 | 0.822682 | 2.08E-53 | -1.69E-46 |
| MS.gene055441 | MS.gene63902 | 0.816612 | 5.06E-52 | -1.69E-46 |
| MS.gene055512 | MS.gene63902 | 0.815749 | 7.88E-52 | -1.69E-46 |
| MS.gene055591 | MS.gene63902 | -0.802819 | 4.68E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene63902 | -0.843043 | 1.79E-58 | -1.69E-46 |
| MS.gene056432 | MS.gene63902 | 0.838748 | 2.39E-57 | -1.69E-46 |
| MS.gene056580 | MS.gene63902 | 0.818466 | 1.93E-52 | -1.69E-46 |
| MS.gene056589 | MS.gene63902 | 0.817518 | 3.16E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene63902 | 0.810803 | 9.61E-51 | -1.69E-46 |
| MS.gene056760 | MS.gene63902 | 0.834121 | 3.60E-56 | -1.69E-46 |
| MS.gene057057 | MS.gene63902 | 0.910864 | 1.16E-82 | -1.69E-46 |
| MS.gene057140 | MS.gene63902 | 0.808273 | 3.36E-50 | -1.69E-46 |
| MS.gene057234 | MS.gene63902 | 0.822888 | 1.86E-53 | -1.69E-46 |
| MS.gene058054 | MS.gene63902 | 0.803606 | 3.22E-49 | -1.69E-46 |
| MS.gene058055 | MS.gene63902 | 0.831877 | 1.30E-55 | -1.69E-46 |
| MS.gene058182 | MS.gene63902 | 0.837406 | 5.30E-57 | -1.69E-46 |
| MS.gene058646 | MS.gene63902 | 0.801456 | 8.93E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene63902 | 0.851568 | 8.15E-61 | -1.69E-46 |
| MS.gene058982 | MS.gene63902 | 0.806048 | 9.94E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene63902 | 0.812425 | 4.27E-51 | -1.69E-46 |
| MS.gene059713 | MS.gene63902 | 0.80328 | 3.76E-49 | -1.69E-46 |
| MS.gene059898 | MS.gene63902 | 0.812404 | 4.31E-51 | -1.69E-46 |
| MS.gene059937 | MS.gene63902 | 0.80216 | 6.40E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene63902 | 0.829502 | 4.97E-55 | -1.69E-46 |
| MS.gene060165 | MS.gene63902 | 0.810351 | 1.20E-50 | -1.69E-46 |
| MS.gene060463 | MS.gene63902 | 0.810678 | 1.02E-50 | -1.69E-46 |
| MS.gene060465 | MS.gene63902 | 0.813159 | 2.95E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene63902 | 0.803213 | 3.88E-49 | -1.69E-46 |
| MS.gene060619 | MS.gene63902 | 0.814981 | 1.17E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGAAAATGATTGGTAAAT+TGG | 0.230977 | 5.1:-76009858 | MS.gene63902:CDS |
| CTTGCACATCCTTGTTCCCT+TGG | 0.337027 | 5.1:+76009360 | None:intergenic |
| CGTAAGATTGAGATATTACT+TGG | 0.360048 | 5.1:-76009638 | MS.gene63902:CDS |
| TAGGAATAAGGTTCGTGTAT+TGG | 0.381629 | 5.1:-76009765 | MS.gene63902:CDS |
| TAAATTGGAGCATGAAGTTT+CGG | 0.399727 | 5.1:-76009843 | MS.gene63902:CDS |
| TTGAAGAGTGTAGGTGTCTC+TGG | 0.402552 | 5.1:-76009548 | MS.gene63902:CDS |
| AAGGAAACTATAAGTAGGTT+GGG | 0.404054 | 5.1:-76009692 | MS.gene63902:CDS |
| TCCTCTAAATTTCTTGTACA+TGG | 0.413034 | 5.1:+76009613 | None:intergenic |
| GAAGGAAACTATAAGTAGGT+TGG | 0.440805 | 5.1:-76009693 | MS.gene63902:CDS |
| GTCCCTGCTAATCACACTCT+TGG | 0.441563 | 5.1:-76009410 | MS.gene63902:CDS |
| TCCATGTACAAGAAATTTAG+AGG | 0.446165 | 5.1:-76009614 | MS.gene63902:CDS |
| GATCTTCTTGACGATGAGTT+CGG | 0.446687 | 5.1:-76009455 | MS.gene63902:CDS |
| TGAGGAAATCGAAGAAGAAA+TGG | 0.446716 | 5.1:-76009817 | MS.gene63902:CDS |
| TTAGTAGCCATAGGATCAAC+AGG | 0.451539 | 5.1:+76009500 | None:intergenic |
| ACCTACACTCTTCAAAAGAT+TGG | 0.453769 | 5.1:+76009556 | None:intergenic |
| ACTCCAAGAGTGTGATTAGC+AGG | 0.461799 | 5.1:+76009407 | None:intergenic |
| GCTGGACTTGAGGTTAGAAA+TGG | 0.469464 | 5.1:-76009722 | MS.gene63902:CDS |
| GGTTAGAAATGGCGAGTTGA+AGG | 0.474088 | 5.1:-76009711 | MS.gene63902:CDS |
| GAAGTCACCTGTTGATCCTA+TGG | 0.481237 | 5.1:-76009507 | MS.gene63902:CDS |
| GGTTGGAAGACTTTCGAGGT+TGG | 0.484020 | 5.1:-76009588 | MS.gene63902:CDS |
| GATGTGCAAGGTTCATCATC+AGG | 0.498818 | 5.1:-76009347 | None:intergenic |
| AAGAAATTTAGAGGACTGGT+TGG | 0.504312 | 5.1:-76009605 | MS.gene63902:CDS |
| AAATGCTTTGGCTGGACTTG+AGG | 0.508557 | 5.1:-76009732 | MS.gene63902:CDS |
| GTACAAGAAATTTAGAGGAC+TGG | 0.508835 | 5.1:-76009609 | MS.gene63902:CDS |
| TGGAAGACTTTCGAGGTTGG+AGG | 0.517961 | 5.1:-76009585 | MS.gene63902:CDS |
| GAATAAGGTTCGTGTATTGG+AGG | 0.532923 | 5.1:-76009762 | MS.gene63902:CDS |
| GACTGGTTGGAAGACTTTCG+AGG | 0.538485 | 5.1:-76009592 | MS.gene63902:CDS |
| AGTGTAGGTGTCTCTGGTGG+TGG | 0.543623 | 5.1:-76009542 | MS.gene63902:CDS |
| AGTACTCAACCGCAGAGCCA+AGG | 0.547766 | 5.1:-76009377 | MS.gene63902:CDS |
| GGGGAAAGAAAAGAGTAAGT+TGG | 0.547900 | 5.1:-76009672 | MS.gene63902:CDS |
| TATTGAAGACAGAGATGAAG+AGG | 0.548764 | 5.1:-76009477 | MS.gene63902:CDS |
| TCCTTGTTCCCTTGGCTCTG+CGG | 0.558940 | 5.1:+76009368 | None:intergenic |
| CTCTGGTGGTGGTAGTAACG+AGG | 0.560560 | 5.1:-76009531 | MS.gene63902:CDS |
| AGCATGAAGTTTCGGAGCTG+AGG | 0.564279 | 5.1:-76009835 | MS.gene63902:CDS |
| GGTGTGTTGAGGAAAATGAT+TGG | 0.569444 | 5.1:-76009866 | None:intergenic |
| AATAAGGTTCGTGTATTGGA+GGG | 0.576354 | 5.1:-76009761 | MS.gene63902:CDS |
| ACCGCAGAGCCAAGGGAACA+AGG | 0.578207 | 5.1:-76009369 | MS.gene63902:CDS |
| AGGAAACTATAAGTAGGTTG+GGG | 0.587533 | 5.1:-76009691 | MS.gene63902:CDS |
| AGTTGAAGGAAACTATAAGT+AGG | 0.591344 | 5.1:-76009697 | MS.gene63902:CDS |
| GTACTCAACCGCAGAGCCAA+GGG | 0.604360 | 5.1:-76009376 | MS.gene63902:CDS |
| CGGAAATGACACTGTTGAGG+AGG | 0.614193 | 5.1:-76009435 | MS.gene63902:CDS |
| TCTGGTGGTGGTAGTAACGA+GGG | 0.624844 | 5.1:-76009530 | MS.gene63902:CDS |
| TCTTCAATATTAGTAGCCAT+AGG | 0.636021 | 5.1:+76009491 | None:intergenic |
| CAAGGGAACAAGGATGTGCA+AGG | 0.636506 | 5.1:-76009359 | MS.gene63902:CDS |
| GTTCGGAAATGACACTGTTG+AGG | 0.644475 | 5.1:-76009438 | MS.gene63902:CDS |
| CTGGTGGTGGTAGTAACGAG+GGG | 0.663660 | 5.1:-76009529 | MS.gene63902:CDS |
| CTCCAAGAGTGTGATTAGCA+GGG | 0.666199 | 5.1:+76009408 | None:intergenic |
| GTAAGATTGAGATATTACTT+GGG | 0.675489 | 5.1:-76009637 | MS.gene63902:CDS |
| AAGAGTGTAGGTGTCTCTGG+TGG | 0.688326 | 5.1:-76009545 | MS.gene63902:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTAAGATTGAGATATTACTT+GGG | - | chr5.1:76009566-76009585 | MS.gene63902:CDS | 25.0% |
| AAGGAAACTATAAGTAGGTT+GGG | - | chr5.1:76009511-76009530 | MS.gene63902:CDS | 30.0% | |
| AGTTGAAGGAAACTATAAGT+AGG | - | chr5.1:76009506-76009525 | MS.gene63902:CDS | 30.0% | |
| CGTAAGATTGAGATATTACT+TGG | - | chr5.1:76009565-76009584 | MS.gene63902:CDS | 30.0% | |
| TAAATTGGAGCATGAAGTTT+CGG | - | chr5.1:76009360-76009379 | MS.gene63902:CDS | 30.0% | |
| TCCATGTACAAGAAATTTAG+AGG | - | chr5.1:76009589-76009608 | MS.gene63902:CDS | 30.0% | |
| TCCTCTAAATTTCTTGTACA+TGG | + | chr5.1:76009593-76009612 | None:intergenic | 30.0% | |
| TCTTCAATATTAGTAGCCAT+AGG | + | chr5.1:76009715-76009734 | None:intergenic | 30.0% | |
| AATAAGGTTCGTGTATTGGA+GGG | - | chr5.1:76009442-76009461 | MS.gene63902:CDS | 35.0% | |
| ACCTACACTCTTCAAAAGAT+TGG | + | chr5.1:76009650-76009669 | None:intergenic | 35.0% | |
| AGGAAACTATAAGTAGGTTG+GGG | - | chr5.1:76009512-76009531 | MS.gene63902:CDS | 35.0% | |
| GAAGGAAACTATAAGTAGGT+TGG | - | chr5.1:76009510-76009529 | MS.gene63902:CDS | 35.0% | |
| GTACAAGAAATTTAGAGGAC+TGG | - | chr5.1:76009594-76009613 | MS.gene63902:CDS | 35.0% | |
| TAGGAATAAGGTTCGTGTAT+TGG | - | chr5.1:76009438-76009457 | MS.gene63902:CDS | 35.0% | |
| TATTGAAGACAGAGATGAAG+AGG | - | chr5.1:76009726-76009745 | MS.gene63902:CDS | 35.0% | |
| TGAGGAAATCGAAGAAGAAA+TGG | - | chr5.1:76009386-76009405 | MS.gene63902:CDS | 35.0% | |
| ! | AAGAAATTTAGAGGACTGGT+TGG | - | chr5.1:76009598-76009617 | MS.gene63902:CDS | 35.0% |
| ! | TTTTGATGCGCTTAGGAATA+AGG | - | chr5.1:76009426-76009445 | MS.gene63902:CDS | 35.0% |
| !! | ACCAATCTTTTGAAGAGTGT+AGG | - | chr5.1:76009646-76009665 | MS.gene63902:CDS | 35.0% |
| GAATAAGGTTCGTGTATTGG+AGG | - | chr5.1:76009441-76009460 | MS.gene63902:CDS | 40.0% | |
| GATCTTCTTGACGATGAGTT+CGG | - | chr5.1:76009748-76009767 | MS.gene63902:CDS | 40.0% | |
| GGAGGGTGATAAAAATGCTT+TGG | - | chr5.1:76009459-76009478 | MS.gene63902:CDS | 40.0% | |
| GGGGAAAGAAAAGAGTAAGT+TGG | - | chr5.1:76009531-76009550 | MS.gene63902:CDS | 40.0% | |
| GGTGATAAAAATGCTTTGGC+TGG | - | chr5.1:76009463-76009482 | MS.gene63902:CDS | 40.0% | |
| TTAGTAGCCATAGGATCAAC+AGG | + | chr5.1:76009706-76009725 | None:intergenic | 40.0% | |
| AAATGCTTTGGCTGGACTTG+AGG | - | chr5.1:76009471-76009490 | MS.gene63902:CDS | 45.0% | |
| GAAGTCACCTGTTGATCCTA+TGG | - | chr5.1:76009696-76009715 | MS.gene63902:CDS | 45.0% | |
| GATGTGCAAGGTTCATCATC+AGG | - | chr5.1:76009856-76009875 | MS.gene63902:CDS | 45.0% | |
| GCTGGACTTGAGGTTAGAAA+TGG | - | chr5.1:76009481-76009500 | MS.gene63902:CDS | 45.0% | |
| GTTCGGAAATGACACTGTTG+AGG | - | chr5.1:76009765-76009784 | MS.gene63902:CDS | 45.0% | |
| ! | ACTCCAAGAGTGTGATTAGC+AGG | + | chr5.1:76009799-76009818 | None:intergenic | 45.0% |
| ! | CTCCAAGAGTGTGATTAGCA+GGG | + | chr5.1:76009798-76009817 | None:intergenic | 45.0% |
| ! | GCAATGCTTTTGATGCGCTT+AGG | - | chr5.1:76009419-76009438 | MS.gene63902:CDS | 45.0% |
| ! | GGTTAGAAATGGCGAGTTGA+AGG | - | chr5.1:76009492-76009511 | MS.gene63902:CDS | 45.0% |
| ! | TTGAAGAGTGTAGGTGTCTC+TGG | - | chr5.1:76009655-76009674 | MS.gene63902:CDS | 45.0% |
| AGCATGAAGTTTCGGAGCTG+AGG | - | chr5.1:76009368-76009387 | MS.gene63902:CDS | 50.0% | |
| CAAGGGAACAAGGATGTGCA+AGG | - | chr5.1:76009844-76009863 | MS.gene63902:CDS | 50.0% | |
| CGGAAATGACACTGTTGAGG+AGG | - | chr5.1:76009768-76009787 | MS.gene63902:CDS | 50.0% | |
| CTTGCACATCCTTGTTCCCT+TGG | + | chr5.1:76009846-76009865 | None:intergenic | 50.0% | |
| GGTTGGAAGACTTTCGAGGT+TGG | - | chr5.1:76009615-76009634 | MS.gene63902:CDS | 50.0% | |
| GTCCCTGCTAATCACACTCT+TGG | - | chr5.1:76009793-76009812 | MS.gene63902:CDS | 50.0% | |
| TGGAAGACTTTCGAGGTTGG+AGG | - | chr5.1:76009618-76009637 | MS.gene63902:CDS | 50.0% | |
| ! | AAGAGTGTAGGTGTCTCTGG+TGG | - | chr5.1:76009658-76009677 | MS.gene63902:CDS | 50.0% |
| ! | GACTGGTTGGAAGACTTTCG+AGG | - | chr5.1:76009611-76009630 | MS.gene63902:CDS | 50.0% |
| !! | TCTGGTGGTGGTAGTAACGA+GGG | - | chr5.1:76009673-76009692 | MS.gene63902:CDS | 50.0% |
| AGTACTCAACCGCAGAGCCA+AGG | - | chr5.1:76009826-76009845 | MS.gene63902:CDS | 55.0% | |
| AGTGTAGGTGTCTCTGGTGG+TGG | - | chr5.1:76009661-76009680 | MS.gene63902:CDS | 55.0% | |
| GTACTCAACCGCAGAGCCAA+GGG | - | chr5.1:76009827-76009846 | MS.gene63902:CDS | 55.0% | |
| !! | CTCTGGTGGTGGTAGTAACG+AGG | - | chr5.1:76009672-76009691 | MS.gene63902:CDS | 55.0% |
| !! | CTGGTGGTGGTAGTAACGAG+GGG | - | chr5.1:76009674-76009693 | MS.gene63902:CDS | 55.0% |
| !! | TCCTTGTTCCCTTGGCTCTG+CGG | + | chr5.1:76009838-76009857 | None:intergenic | 55.0% |
| ACCGCAGAGCCAAGGGAACA+AGG | - | chr5.1:76009834-76009853 | MS.gene63902:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 76009348 | 76009877 | 76009348 | ID=MS.gene63902 |
| chr5.1 | mRNA | 76009348 | 76009877 | 76009348 | ID=MS.gene63902.t1;Parent=MS.gene63902 |
| chr5.1 | exon | 76009348 | 76009877 | 76009348 | ID=MS.gene63902.t1.exon1;Parent=MS.gene63902.t1 |
| chr5.1 | CDS | 76009348 | 76009877 | 76009348 | ID=cds.MS.gene63902.t1;Parent=MS.gene63902.t1 |
| Gene Sequence |
| Protein sequence |
