AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64710.t1 MTR_5g021090 96.124 129 5 0 1 129 1 129 5.92e-91 259
MS.gene64710.t1 MTR_2g038560 59.223 103 42 0 24 126 19 121 9.88e-41 132
MS.gene64710.t1 MTR_2g038560 59.223 103 42 0 24 126 60 162 3.26e-40 132
MS.gene64710.t1 MTR_2g038560 59.223 103 42 0 24 126 63 165 3.67e-40 132
MS.gene64710.t1 MTR_7g035245 45.556 90 49 0 40 129 10 99 3.57e-24 89.7
MS.gene64710.t1 MTR_3g077570 43.182 88 50 0 42 129 27 114 2.79e-20 80.1
MS.gene64710.t1 MTR_1g069255 40.217 92 54 1 36 126 86 177 3.24e-19 79.3
MS.gene64710.t1 MTR_3g077560 42.353 85 49 0 42 126 30 114 5.35e-19 77.0
MS.gene64710.t1 MTR_7g108260 37.209 86 53 1 45 129 13 98 7.26e-12 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64710.t1 AT1G77370 67.500 120 39 0 10 129 11 130 3.48e-54 166
MS.gene64710.t1 AT1G77370 55.479 146 39 1 10 129 11 156 2.76e-48 152
MS.gene64710.t1 AT5G20500 52.500 120 57 0 7 126 6 125 2.37e-41 134
MS.gene64710.t1 AT5G40370 44.828 87 48 0 40 126 10 96 5.44e-22 84.3
MS.gene64710.t1 AT5G40370 47.500 80 42 0 47 126 42 121 1.21e-19 79.0
MS.gene64710.t1 AT4G28730 43.478 92 51 1 36 126 73 164 1.25e-19 80.1
MS.gene64710.t1 AT2G20270 40.217 92 54 1 36 126 78 169 2.91e-18 76.6
MS.gene64710.t1 AT5G63030 42.683 82 47 0 45 126 31 112 9.52e-18 73.9
MS.gene64710.t1 AT4G28730 44.118 68 37 1 36 102 73 140 1.96e-12 60.5

Find 23 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACATTCAGGGTGTTCTTC+TGG 0.230382 5.2:+12300293 MS.gene64710:CDS
GGTTCCATTCTCAAGCAAAA+AGG 0.276134 5.2:-12299452 None:intergenic
AGATGATGGGTATCACATTC+AGG 0.282138 5.2:+12300281 MS.gene64710:intron
GTCCCACAAGTGTTTGTCTA+TGG 0.290060 5.2:+12300336 MS.gene64710:CDS
TACGTACGGGCAATAGGATT+TGG 0.352701 5.2:-12299542 None:intergenic
TATATATACGTACGGGCAAT+AGG 0.386425 5.2:-12299548 None:intergenic
GGTGTTCTTCTGGATTTGAT+TGG 0.395517 5.2:+12300303 MS.gene64710:CDS
GGAAACTGAATTTGAAGCTT+CGG 0.429054 5.2:-12299473 None:intergenic
TCTGTGCTTGTTTAGATGAT+GGG 0.457786 5.2:+12300268 MS.gene64710:intron
TCCCATAGACAAACACTTGT+GGG 0.486540 5.2:-12300338 None:intergenic
GATGATGGGTATCACATTCA+GGG 0.487362 5.2:+12300282 MS.gene64710:intron
TTTGTCTATGGGAAGCATAT+TGG 0.501883 5.2:+12300348 MS.gene64710:CDS
AAATCAAGCTCAACAACAAA+AGG 0.509148 5.2:-12299694 None:intergenic
GTCTATGGGAAGCATATTGG+TGG 0.513655 5.2:+12300351 MS.gene64710:CDS
GGAGAAGATGGTGATTCTGT+TGG 0.515760 5.2:-12299521 None:intergenic
GCAATAGGATTTGGAGAAGA+TGG 0.516584 5.2:-12299533 None:intergenic
TCCCACAAGTGTTTGTCTAT+GGG 0.535106 5.2:+12300337 MS.gene64710:CDS
AAGCATATTGGTGGATCAGA+TGG 0.559694 5.2:+12300360 MS.gene64710:CDS
GTTTCTGCAATTCACCACTC+TGG 0.562376 5.2:-12301180 None:intergenic
TTCCCATAGACAAACACTTG+TGG 0.621034 5.2:-12300339 None:intergenic
GAAGAATGAAGATGTTACAG+TGG 0.663384 5.2:+12299392 None:intergenic
GTTGTTGAGCTTGATTTGCG+AGG 0.668245 5.2:+12299700 MS.gene64710:CDS
CTCAGTGCTGCAGTCCAGAG+TGG 0.673667 5.2:+12301166 MS.gene64710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAACTGCTTTAA+TGG + chr5.2:12300083-12300102 MS.gene64710:intron 15.0%
!! TATCTATATGTCATTATAAA+AGG + chr5.2:12301079-12301098 MS.gene64710:intron 15.0%
!!! AAGGGAATATTTATTATTTT+CGG - chr5.2:12300906-12300925 None:intergenic 15.0%
!!! TTTTTTTCCATATACTTTAA+AGG + chr5.2:12300997-12301016 MS.gene64710:intron 15.0%
!! CTCTATATCCAAAAATATAA+GGG + chr5.2:12300820-12300839 MS.gene64710:intron 20.0%
!! TAAACTATATGAAAATAACG+AGG + chr5.2:12300767-12300786 MS.gene64710:intron 20.0%
!! TATGTCATTATAAAAGGTTA+AGG + chr5.2:12301085-12301104 MS.gene64710:intron 20.0%
!! TCATATAGTTTAAGAACTAA+AGG - chr5.2:12300759-12300778 None:intergenic 20.0%
!!! ATTAAGTTGTTTATCAAACA+AGG - chr5.2:12299769-12299788 None:intergenic 20.0%
!!! CTTATATTTTTGGATATAGA+GGG - chr5.2:12300821-12300840 None:intergenic 20.0%
!!! TATGTGTTTTGATATTTCTA+AGG + chr5.2:12299910-12299929 MS.gene64710:intron 20.0%
!!! TATTTAGCCTTTAAAGTATA+TGG - chr5.2:12301007-12301026 None:intergenic 20.0%
!!! TGATAGAAATTTTTTATGAG+AGG + chr5.2:12300872-12300891 MS.gene64710:intron 20.0%
! AAGAGAGTTATATATACGTA+CGG - chr5.2:12299559-12299578 None:intergenic 25.0%
! AATGATAACAGTTGTCTATT+TGG - chr5.2:12300216-12300235 None:intergenic 25.0%
! AGAAATAACCTATACATAAG+AGG - chr5.2:12300005-12300024 None:intergenic 25.0%
! ATTCAAATAGTATACTCAGT+CGG - chr5.2:12299968-12299987 None:intergenic 25.0%
! CCTCTATATCCAAAAATATA+AGG + chr5.2:12300819-12300838 MS.gene64710:intron 25.0%
! GAAATAACCTATACATAAGA+GGG - chr5.2:12300004-12300023 None:intergenic 25.0%
! GTTGTGATATTCATCAATTA+AGG - chr5.2:12299889-12299908 None:intergenic 25.0%
! GTTTAATTAATATGCTCTGT+CGG + chr5.2:12299822-12299841 MS.gene64710:intron 25.0%
! TAATGAAGGGATTAAATTGA+AGG + chr5.2:12301040-12301059 MS.gene64710:intron 25.0%
! TATATCCAAAAATATAAGGG+TGG + chr5.2:12300823-12300842 MS.gene64710:intron 25.0%
! TATGAAAATAACGAGGAATT+TGG + chr5.2:12300774-12300793 MS.gene64710:intron 25.0%
! TCAATTATTATGACGTGTTT+GGG + chr5.2:12299585-12299604 MS.gene64710:intron 25.0%
! TTCAATTATTATGACGTGTT+TGG + chr5.2:12299584-12299603 MS.gene64710:intron 25.0%
!! ACAAATTTTCACATACTCAA+GGG - chr5.2:12300924-12300943 None:intergenic 25.0%
!! ATACTTTAAAGGCTAAATAG+AGG + chr5.2:12301008-12301027 MS.gene64710:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+AGG - chr5.2:12300485-12300504 None:intergenic 25.0%
!! TTTTCATAAGTGTTTATGTC+AGG + chr5.2:12300168-12300187 MS.gene64710:intron 25.0%
!!! CCTTATATTTTTGGATATAG+AGG - chr5.2:12300822-12300841 None:intergenic 25.0%
AAATCAAGCTCAACAACAAA+AGG - chr5.2:12299697-12299716 None:intergenic 30.0%
AGAGAGTTATATATACGTAC+GGG - chr5.2:12299558-12299577 None:intergenic 30.0%
ATGAGCAAACTTTGTAAGAA+TGG - chr5.2:12301132-12301151 None:intergenic 30.0%
ATGGATAAAGAAACACAAAG+AGG - chr5.2:12301113-12301132 None:intergenic 30.0%
CATTAGTACTTAAGATGCTA+GGG - chr5.2:12300956-12300975 None:intergenic 30.0%
TAACAGTTGTCTATTTGGAT+TGG - chr5.2:12300211-12300230 None:intergenic 30.0%
TCATTAGTACTTAAGATGCT+AGG - chr5.2:12300957-12300976 None:intergenic 30.0%
TCTTAAGTACTAATGAGACA+AGG + chr5.2:12300961-12300980 MS.gene64710:intron 30.0%
TGGATAAAGAAACACAAAGA+GGG - chr5.2:12301112-12301131 None:intergenic 30.0%
TTTCATACTTAGTCCTTGTT+TGG + chr5.2:12300469-12300488 MS.gene64710:intron 30.0%
! AATGTTTCTCTGATTTTCGT+AGG + chr5.2:12299626-12299645 MS.gene64710:intron 30.0%
! ACTTTCCACCCTTATATTTT+TGG - chr5.2:12300831-12300850 None:intergenic 30.0%
! AGAGGAGATAATGATAATGA+AGG + chr5.2:12301026-12301045 MS.gene64710:intron 30.0%
! GACAAATTTTCACATACTCA+AGG - chr5.2:12300925-12300944 None:intergenic 30.0%
! GAGGAGATAATGATAATGAA+GGG + chr5.2:12301027-12301046 MS.gene64710:intron 30.0%
!! TCTTAGACTTTTGCTTTTCT+GGG + chr5.2:12300395-12300414 MS.gene64710:intron 30.0%
!! TTCTTAGACTTTTGCTTTTC+TGG + chr5.2:12300394-12300413 MS.gene64710:intron 30.0%
CTCAAGCAAAAAGGGTAAAA+CGG - chr5.2:12299446-12299465 None:intergenic 35.0%
GTTCCATTCTCAAGCAAAAA+GGG - chr5.2:12299454-12299473 None:intergenic 35.0%
TAAGGAGACAACACAATCAT+CGG - chr5.2:12299871-12299890 None:intergenic 35.0%
TATATATACGTACGGGCAAT+AGG - chr5.2:12299551-12299570 None:intergenic 35.0%
TCTGTGCTTGTTTAGATGAT+GGG + chr5.2:12300268-12300287 MS.gene64710:intron 35.0%
TTAAGATGCTAGGGATTAAC+AGG - chr5.2:12300947-12300966 None:intergenic 35.0%
TTCTGTGCTTGTTTAGATGA+TGG + chr5.2:12300267-12300286 MS.gene64710:intron 35.0%
! ATTGTTTTCGCGTAAGCTAA+AGG + chr5.2:12300039-12300058 MS.gene64710:intron 35.0%
! GGAAACTGAATTTGAAGCTT+CGG - chr5.2:12299476-12299495 None:intergenic 35.0%
! TTACCCTTTTTGCTTGAGAA+TGG + chr5.2:12299448-12299467 MS.gene64710:CDS 35.0%
! TTTGTCTATGGGAAGCATAT+TGG + chr5.2:12300348-12300367 MS.gene64710:CDS 35.0%
!! GATTGCGTTTTGAACAAAAG+CGG - chr5.2:12299497-12299516 None:intergenic 35.0%
ACTGCTTTAATGGTCTGTCA+TGG + chr5.2:12300093-12300112 MS.gene64710:intron 40.0%
CCTGATACCCTCTTATGTAT+AGG + chr5.2:12299994-12300013 MS.gene64710:intron 40.0%
GCAATAGGATTTGGAGAAGA+TGG - chr5.2:12299536-12299555 None:intergenic 40.0%
GGTGTTCTTCTGGATTTGAT+TGG + chr5.2:12300303-12300322 MS.gene64710:CDS 40.0%
GGTTCCATTCTCAAGCAAAA+AGG - chr5.2:12299455-12299474 None:intergenic 40.0%
GTGGAAAGTGAGAGAAGATA+GGG + chr5.2:12300842-12300861 MS.gene64710:intron 40.0%
TCCCACAAGTGTTTGTCTAT+GGG + chr5.2:12300337-12300356 MS.gene64710:CDS 40.0%
TCCCATAGACAAACACTTGT+GGG - chr5.2:12300341-12300360 None:intergenic 40.0%
TTCCCATAGACAAACACTTG+TGG - chr5.2:12300342-12300361 None:intergenic 40.0%
! AAGCATATTGGTGGATCAGA+TGG + chr5.2:12300360-12300379 MS.gene64710:CDS 40.0%
!! AGATGATGGGTATCACATTC+AGG + chr5.2:12300281-12300300 MS.gene64710:intron 40.0%
!! CCTATACATAAGAGGGTATC+AGG - chr5.2:12299997-12300016 None:intergenic 40.0%
!! GATGATGGGTATCACATTCA+GGG + chr5.2:12300282-12300301 MS.gene64710:intron 40.0%
AGGGATGCATGTTTGAGAGA+GGG - chr5.2:12300802-12300821 None:intergenic 45.0%
GGAGAAGATGGTGATTCTGT+TGG - chr5.2:12299524-12299543 None:intergenic 45.0%
GGTGGAAAGTGAGAGAAGAT+AGG + chr5.2:12300841-12300860 MS.gene64710:intron 45.0%
GTCCCACAAGTGTTTGTCTA+TGG + chr5.2:12300336-12300355 MS.gene64710:CDS 45.0%
GTTTCTGCAATTCACCACTC+TGG - chr5.2:12301183-12301202 None:intergenic 45.0%
TACGTACGGGCAATAGGATT+TGG - chr5.2:12299545-12299564 None:intergenic 45.0%
! GTCTATGGGAAGCATATTGG+TGG + chr5.2:12300351-12300370 MS.gene64710:CDS 45.0%
! TCACATTCAGGGTGTTCTTC+TGG + chr5.2:12300293-12300312 MS.gene64710:CDS 45.0%
!! GTTGTTGAGCTTGATTTGCG+AGG + chr5.2:12299700-12299719 MS.gene64710:CDS 45.0%
GAGGGATGCATGTTTGAGAG+AGG - chr5.2:12300803-12300822 None:intergenic 50.0%
CTCAGTGCTGCAGTCCAGAG+TGG + chr5.2:12301166-12301185 MS.gene64710:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 12299397 12301219 12299397 ID=MS.gene64710
chr5.2 mRNA 12299397 12301219 12299397 ID=MS.gene64710.t1;Parent=MS.gene64710
chr5.2 exon 12299397 12299557 12299397 ID=MS.gene64710.t1.exon1;Parent=MS.gene64710.t1
chr5.2 CDS 12299397 12299557 12299397 ID=cds.MS.gene64710.t1;Parent=MS.gene64710.t1
chr5.2 exon 12299648 12299721 12299648 ID=MS.gene64710.t1.exon2;Parent=MS.gene64710.t1
chr5.2 CDS 12299648 12299721 12299648 ID=cds.MS.gene64710.t1;Parent=MS.gene64710.t1
chr5.2 exon 12300283 12300381 12300283 ID=MS.gene64710.t1.exon3;Parent=MS.gene64710.t1
chr5.2 CDS 12300283 12300381 12300283 ID=cds.MS.gene64710.t1;Parent=MS.gene64710.t1
chr5.2 exon 12301164 12301219 12301164 ID=MS.gene64710.t1.exon4;Parent=MS.gene64710.t1
chr5.2 CDS 12301164 12301219 12301164 ID=cds.MS.gene64710.t1;Parent=MS.gene64710.t1
Gene Sequence

>MS.gene64710

ATGAAGATGTTACAGTGGAGCATGTTCATAACTCTCACGTTATTGACCGTTTTACCCTTTTTGCTTGAGAATGGAACCGAAGCTTCAAATTCAGTTTCCGCTTTTGTTCAAAACGCAATCTACTCCAACAGAATCACCATCTTCTCCAAATCCTATTGCCCGTACGTATATATAACTCTCTTCAATTTTCAATTATTATGACGTGTTTGGGTTTGTTCAATTTCATCACAATGTTTCTCTGATTTTCGTAGGTATTGTTTACGTGCTAAACGTGTTTTTGTTGAACTAAATGAGCAACCTTTTGTTGTTGAGCTTGATTTGCGAGGTATCTCTTAAATCATCACTCTGTTATATTTATGTTTTGTGTCGCCTTGTTTGATAAACAACTTAATTTGCAGCTTATAGTGTAAGTGTTTTTCATGATAGTTTAATTAATATGCTCTGTCGGTGTACAATATTTTACACATTCATCCGATGATTGTGTTGTCTCCTTAATTGATGAATATCACAACTTATGTGTTTTGATATTTCTAAGGACATTATTAGATGTGTGTAAAATAATTGTACACCGACTGAGTATACTATTTGAATTCTTGTCCTGATACCCTCTTATGTATAGGTTATTTCTATAACAAAAGTTAAATTGTTTTCGCGTAAGCTAAAGGCTGCTTTCATTTGCTCATAAAAAAAAAAAAAACTGCTTTAATGGTCTGTCATGGAGAGCTTGTGAAAATAAGCTGAAAACAGATTATGAGCACATGTCGTACGCTGTTTTCATAAGTGTTTATGTCAGGAAATAAGTTCAAATAAGCCAATCCAAATAGACAACTGTTATCATTATTCAAACTTATCTCACGCTCGTCGTAATTTTTCTGTGCTTGTTTAGATGATGGGTATCACATTCAGGGTGTTCTTCTGGATTTGATTGGTAGAAGAACAGTCCCACAAGTGTTTGTCTATGGGAAGCATATTGGTGGATCAGATGGTTAGTTTTTGCTTCTTAGACTTTTGCTTTTCTGGGTTACTATTATCTAAGATTAAACTCTCAATTTAGTAGTCAAGTATCACACTCTTTCATACTTAGTCCTTGTTTGGATAAACAACTTAATTTGTAGCTTTTAGTATAAGTTCTTATCATGATAAGCGCTTATGTATAAGCTATTTCTATAACAACAACAAAAAAGTTGCATTCTTTTCGTATAATCTATAAGCTGTTTTCATAAGCTATCTTGTATAGCAAATGAAAATAAGCTGAAAACAACTTATAAACAATGTCATAAGCTATTTTCATATATTCTCTCAAACAGACTCACAAAACTTATGTTAATAGATAAGCTCAAATAAGTCAGTTCAAACAAGCCTTTAGTTCTTAAACTATATGAAAATAACGAGGAATTTGGATCCCTCTCTCAAACATGCATCCCTCTATATCCAAAAATATAAGGGTGGAAAGTGAGAGAAGATAGGGACTTAATTGATAGAAATTTTTTATGAGAGGATCTAAATCCGAAAATAATAAATATTCCCTTGAGTATGTGAAAATTTGTCCTGTTAATCCCTAGCATCTTAAGTACTAATGAGACAAGGCGCTACTTACTTTTTTTTTTCCATATACTTTAAAGGCTAAATAGAGGAGATAATGATAATGAAGGGATTAAATTGAAGGTTTAGTGTATTGCTATTATCTATATGTCATTATAAAAGGTTAAGGTCCCTCTTTGTGTTTCTTTATCCATTCTTACAAAGTTTGCTCATTTTCTAATTTAGATCTCAGTGCTGCAGTCCAGAGTGGTGAATTGCAGAAACTTCTCAAATCAAGTTGA

Protein sequence

>MS.gene64710.t1

MKMLQWSMFITLTLLTVLPFLLENGTEASNSVSAFVQNAIYSNRITIFSKSYCPYCLRAKRVFVELNEQPFVVELDLRDDGYHIQGVLLDLIGRRTVPQVFVYGKHIGGSDDLSAAVQSGELQKLLKSS