Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65295.t1 | XP_003606498.1 | 97.5 | 118 | 3 | 0 | 1 | 118 | 54 | 171 | 1.40E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65295.t1 | Q9M1E7 | 77.1 | 118 | 27 | 0 | 1 | 118 | 54 | 171 | 5.1e-49 | 194.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65295.t1 | G7JRH3 | 97.5 | 118 | 3 | 0 | 1 | 118 | 54 | 171 | 1.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04919 | MS.gene65295 | 0.804805 | 1.81E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene65295 | 0.829042 | 6.42E-55 | -1.69E-46 |
| MS.gene049283 | MS.gene65295 | 0.87452 | 6.33E-68 | -1.69E-46 |
| MS.gene049499 | MS.gene65295 | 0.844899 | 5.68E-59 | -1.69E-46 |
| MS.gene049861 | MS.gene65295 | 0.817021 | 4.09E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene65295 | 0.81344 | 2.56E-51 | -1.69E-46 |
| MS.gene050271 | MS.gene65295 | 0.800465 | 1.42E-48 | -1.69E-46 |
| MS.gene050300 | MS.gene65295 | -0.802783 | 4.76E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene65295 | 0.803093 | 4.11E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene65295 | 0.822788 | 1.96E-53 | -1.69E-46 |
| MS.gene051597 | MS.gene65295 | 0.838821 | 2.29E-57 | -1.69E-46 |
| MS.gene051699 | MS.gene65295 | 0.834855 | 2.35E-56 | -1.69E-46 |
| MS.gene051832 | MS.gene65295 | 0.822571 | 2.21E-53 | -1.69E-46 |
| MS.gene051975 | MS.gene65295 | 0.800658 | 1.30E-48 | -1.69E-46 |
| MS.gene052242 | MS.gene65295 | -0.821968 | 3.04E-53 | -1.69E-46 |
| MS.gene052686 | MS.gene65295 | 0.813671 | 2.27E-51 | -1.69E-46 |
| MS.gene052818 | MS.gene65295 | -0.810385 | 1.18E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene65295 | 0.804648 | 1.95E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene65295 | 0.814094 | 1.84E-51 | -1.69E-46 |
| MS.gene053298 | MS.gene65295 | 0.83758 | 4.78E-57 | -1.69E-46 |
| MS.gene053340 | MS.gene65295 | 0.810204 | 1.29E-50 | -1.69E-46 |
| MS.gene053456 | MS.gene65295 | 0.805561 | 1.26E-49 | -1.69E-46 |
| MS.gene053726 | MS.gene65295 | 0.806583 | 7.67E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene65295 | 0.875369 | 3.25E-68 | -1.69E-46 |
| MS.gene054222 | MS.gene65295 | 0.846909 | 1.61E-59 | -1.69E-46 |
| MS.gene054328 | MS.gene65295 | 0.808963 | 2.39E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene65295 | 0.80626 | 8.97E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene65295 | 0.825462 | 4.63E-54 | -1.69E-46 |
| MS.gene054893 | MS.gene65295 | 0.831776 | 1.38E-55 | -1.69E-46 |
| MS.gene054957 | MS.gene65295 | 0.831957 | 1.24E-55 | -1.69E-46 |
| MS.gene056045 | MS.gene65295 | 0.802721 | 4.90E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene65295 | 0.814931 | 1.20E-51 | -1.69E-46 |
| MS.gene056605 | MS.gene65295 | 0.812207 | 4.76E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene65295 | 0.808646 | 2.80E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene65295 | 0.838117 | 3.48E-57 | -1.69E-46 |
| MS.gene057155 | MS.gene65295 | 0.824697 | 7.01E-54 | -1.69E-46 |
| MS.gene057622 | MS.gene65295 | 0.815504 | 8.94E-52 | -1.69E-46 |
| MS.gene05781 | MS.gene65295 | 0.824832 | 6.52E-54 | -1.69E-46 |
| MS.gene057893 | MS.gene65295 | -0.813332 | 2.70E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene65295 | 0.83049 | 2.85E-55 | -1.69E-46 |
| MS.gene058159 | MS.gene65295 | 0.833331 | 5.67E-56 | -1.69E-46 |
| MS.gene058279 | MS.gene65295 | 0.812571 | 3.96E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene65295 | 0.836743 | 7.83E-57 | -1.69E-46 |
| MS.gene05878 | MS.gene65295 | 0.822172 | 2.73E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene65295 | 0.81139 | 7.17E-51 | -1.69E-46 |
| MS.gene05952 | MS.gene65295 | 0.862795 | 3.93E-64 | -1.69E-46 |
| MS.gene05985 | MS.gene65295 | -0.83799 | 3.75E-57 | -1.69E-46 |
| MS.gene059901 | MS.gene65295 | -0.820008 | 8.60E-53 | -1.69E-46 |
| MS.gene060012 | MS.gene65295 | 0.827454 | 1.55E-54 | -1.69E-46 |
| MS.gene06011 | MS.gene65295 | 0.805003 | 1.65E-49 | -1.69E-46 |
| MS.gene06018 | MS.gene65295 | 0.851655 | 7.71E-61 | -1.69E-46 |
| MS.gene060365 | MS.gene65295 | 0.80677 | 7.00E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene65295 | 0.814355 | 1.61E-51 | -1.69E-46 |
| MS.gene060840 | MS.gene65295 | 0.807435 | 5.06E-50 | -1.69E-46 |
| MS.gene060932 | MS.gene65295 | 0.800645 | 1.31E-48 | -1.69E-46 |
| MS.gene060933 | MS.gene65295 | 0.833973 | 3.92E-56 | -1.69E-46 |
| MS.gene060935 | MS.gene65295 | 0.819182 | 1.33E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene65295 | 0.841842 | 3.72E-58 | -1.69E-46 |
| MS.gene061100 | MS.gene65295 | 0.810122 | 1.35E-50 | -1.69E-46 |
| MS.gene061243 | MS.gene65295 | 0.803538 | 3.32E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65295.t1 | MTR_4g061010 | 97.458 | 118 | 3 | 0 | 1 | 118 | 54 | 171 | 3.73e-81 | 239 |
| MS.gene65295.t1 | MTR_6g033335 | 46.610 | 118 | 57 | 1 | 1 | 118 | 53 | 164 | 2.57e-31 | 112 |
| MS.gene65295.t1 | MTR_4g129670 | 47.458 | 118 | 60 | 1 | 1 | 118 | 53 | 168 | 3.35e-31 | 111 |
| MS.gene65295.t1 | MTR_1g095530 | 44.068 | 118 | 60 | 1 | 1 | 118 | 53 | 164 | 6.24e-23 | 90.5 |
| MS.gene65295.t1 | MTR_8g066790 | 39.474 | 114 | 65 | 1 | 5 | 118 | 57 | 166 | 7.92e-21 | 84.7 |
| MS.gene65295.t1 | MTR_8g097280 | 29.915 | 117 | 78 | 1 | 2 | 118 | 55 | 167 | 4.29e-18 | 77.8 |
| MS.gene65295.t1 | MTR_4g112570 | 36.735 | 98 | 62 | 0 | 1 | 98 | 56 | 153 | 3.11e-17 | 75.1 |
| MS.gene65295.t1 | MTR_8g101600 | 39.316 | 117 | 67 | 1 | 2 | 118 | 54 | 166 | 3.34e-16 | 72.4 |
| MS.gene65295.t1 | MTR_5g030070 | 40.367 | 109 | 61 | 1 | 10 | 118 | 62 | 166 | 3.31e-15 | 69.7 |
| MS.gene65295.t1 | MTR_2g056130 | 35.484 | 93 | 60 | 0 | 2 | 94 | 54 | 146 | 1.25e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65295.t1 | AT3G45600 | 77.119 | 118 | 27 | 0 | 1 | 118 | 54 | 171 | 2.06e-66 | 199 |
| MS.gene65295.t1 | AT3G45600 | 77.119 | 118 | 27 | 0 | 1 | 118 | 54 | 171 | 3.81e-65 | 199 |
| MS.gene65295.t1 | AT5G60220 | 65.217 | 115 | 40 | 0 | 4 | 118 | 57 | 171 | 1.04e-49 | 160 |
| MS.gene65295.t1 | AT2G23810 | 44.915 | 118 | 63 | 1 | 1 | 118 | 54 | 169 | 7.42e-30 | 108 |
| MS.gene65295.t1 | AT3G12090 | 44.915 | 118 | 59 | 2 | 1 | 118 | 53 | 164 | 8.88e-26 | 97.8 |
| MS.gene65295.t1 | AT4G30430 | 45.263 | 95 | 52 | 0 | 1 | 95 | 54 | 148 | 2.93e-24 | 93.6 |
| MS.gene65295.t1 | AT4G28050 | 38.983 | 118 | 70 | 1 | 1 | 118 | 54 | 169 | 1.89e-23 | 91.3 |
| MS.gene65295.t1 | AT5G46700 | 41.379 | 116 | 58 | 3 | 6 | 118 | 58 | 166 | 3.74e-21 | 85.5 |
| MS.gene65295.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 2.95e-20 | 82.0 |
| MS.gene65295.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 4.74e-20 | 81.6 |
| MS.gene65295.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 9.74e-20 | 82.0 |
| MS.gene65295.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 9.74e-20 | 82.0 |
| MS.gene65295.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 1.13e-19 | 81.3 |
| MS.gene65295.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 1.15e-19 | 81.6 |
| MS.gene65295.t1 | AT1G18520 | 37.607 | 117 | 70 | 2 | 2 | 118 | 56 | 169 | 3.52e-19 | 80.5 |
| MS.gene65295.t1 | AT2G19580 | 40.517 | 116 | 64 | 2 | 4 | 118 | 56 | 167 | 8.16e-17 | 73.9 |
| MS.gene65295.t1 | AT4G23410 | 34.783 | 115 | 69 | 1 | 4 | 118 | 56 | 164 | 1.74e-16 | 73.2 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTATGAATAGAGGGTATT+TGG | 0.185250 | 4.3:+41489473 | MS.gene65295:CDS |
| GCTTATGTTGTGACGGATAA+AGG | 0.213333 | 4.3:+41489438 | MS.gene65295:CDS |
| TTAGTCATTGGTGTGCTTAT+TGG | 0.213548 | 4.3:+41489402 | MS.gene65295:CDS |
| GGAAGATTAGTTCTTGTATA+AGG | 0.219711 | 4.3:+41489559 | MS.gene65295:CDS |
| TATTATTTGCAGGATTATTC+TGG | 0.279394 | 4.3:+41489498 | MS.gene65295:CDS |
| AGTGGCTAGTGATGAGTATT+GGG | 0.307047 | 4.3:+41489536 | MS.gene65295:CDS |
| AAGAAAACTCAATCCTATTC+AGG | 0.328328 | 4.3:+41489653 | MS.gene65295:CDS |
| GTATTTGGATTATTATTTGC+AGG | 0.348461 | 4.3:+41489488 | MS.gene65295:CDS |
| GTTGTCATGTTCTTAGTCAT+TGG | 0.349196 | 4.3:+41489390 | MS.gene65295:CDS |
| GAAGATTAGTTCTTGTATAA+GGG | 0.367420 | 4.3:+41489560 | MS.gene65295:CDS |
| ATATAAGAACATACCTGAAT+AGG | 0.397801 | 4.3:-41489666 | None:intergenic |
| CTTATGTTGTGACGGATAAA+GGG | 0.405211 | 4.3:+41489439 | MS.gene65295:CDS |
| GTAACACGCCCCTGCAAAAC+CGG | 0.415261 | 4.3:-41489337 | None:intergenic |
| ATCATGGTTGTTTCATTAGC+CGG | 0.418747 | 4.3:+41489318 | None:intergenic |
| CGGATAAAGGGTCTGGAAGA+AGG | 0.448561 | 4.3:+41489451 | MS.gene65295:CDS |
| TTCTAAAGCTTGTAGGAAGT+TGG | 0.451166 | 4.3:+41489584 | MS.gene65295:CDS |
| TAAGGGATTCTAAAGCTTGT+AGG | 0.452393 | 4.3:+41489577 | MS.gene65295:CDS |
| GAGTGGCTAGTGATGAGTAT+TGG | 0.454709 | 4.3:+41489535 | MS.gene65295:CDS |
| GGAAGAAGGGTTATGAATAG+AGG | 0.468654 | 4.3:+41489465 | MS.gene65295:CDS |
| GTTGTGACGGATAAAGGGTC+TGG | 0.489336 | 4.3:+41489444 | MS.gene65295:CDS |
| AAGAACATATCAGCAGTCTC+AGG | 0.515949 | 4.3:-41489627 | None:intergenic |
| ATTTGCAGGATTATTCTGGT+TGG | 0.516447 | 4.3:+41489502 | MS.gene65295:CDS |
| GCAGGATTATTCTGGTTGGT+TGG | 0.518544 | 4.3:+41489506 | MS.gene65295:CDS |
| AATATTTGCTTATGTTGTGA+CGG | 0.520715 | 4.3:+41489431 | MS.gene65295:CDS |
| GGATAAAGGGTCTGGAAGAA+GGG | 0.556945 | 4.3:+41489452 | MS.gene65295:CDS |
| GTGGCTAGTGATGAGTATTG+GGG | 0.574200 | 4.3:+41489537 | MS.gene65295:CDS |
| GAAGAAGGGTTATGAATAGA+GGG | 0.576688 | 4.3:+41489466 | MS.gene65295:CDS |
| AAGCTTGTAGGAAGTTGGCT+AGG | 0.591037 | 4.3:+41489589 | MS.gene65295:CDS |
| TGGTTGGTTGGAAGAGAGAG+TGG | 0.610133 | 4.3:+41489518 | MS.gene65295:CDS |
| TGGCTAGTGATGAGTATTGG+GGG | 0.638689 | 4.3:+41489538 | MS.gene65295:CDS |
| AACGTAACAAGAAAGTGTTA+CGG | 0.663996 | 4.3:-41489359 | None:intergenic |
| TGACTAAGAACATGACAACA+AGG | 0.676649 | 4.3:-41489386 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATATTTGCTTATGTTGTGA+CGG | + | chr4.3:41489431-41489450 | MS.gene65295:CDS | 25.0% |
| ! | GTATTTGGATTATTATTTGC+AGG | + | chr4.3:41489488-41489507 | MS.gene65295:CDS | 25.0% |
| ! | TATTATTTGCAGGATTATTC+TGG | + | chr4.3:41489498-41489517 | MS.gene65295:CDS | 25.0% |
| !! | GAAGATTAGTTCTTGTATAA+GGG | + | chr4.3:41489560-41489579 | MS.gene65295:CDS | 25.0% |
| ! | GGAAGATTAGTTCTTGTATA+AGG | + | chr4.3:41489559-41489578 | MS.gene65295:CDS | 30.0% |
| !! | AACGTAACAAGAAAGTGTTA+CGG | - | chr4.3:41489362-41489381 | None:intergenic | 30.0% |
| ATTTGCAGGATTATTCTGGT+TGG | + | chr4.3:41489502-41489521 | MS.gene65295:CDS | 35.0% | |
| GAAGAAGGGTTATGAATAGA+GGG | + | chr4.3:41489466-41489485 | MS.gene65295:CDS | 35.0% | |
| GTTGTCATGTTCTTAGTCAT+TGG | + | chr4.3:41489390-41489409 | MS.gene65295:CDS | 35.0% | |
| TGACTAAGAACATGACAACA+AGG | - | chr4.3:41489389-41489408 | None:intergenic | 35.0% | |
| TTAGTCATTGGTGTGCTTAT+TGG | + | chr4.3:41489402-41489421 | MS.gene65295:CDS | 35.0% | |
| !! | CTTATGTTGTGACGGATAAA+GGG | + | chr4.3:41489439-41489458 | MS.gene65295:CDS | 35.0% |
| !! | GGTTATGAATAGAGGGTATT+TGG | + | chr4.3:41489473-41489492 | MS.gene65295:CDS | 35.0% |
| !! | TAAGGGATTCTAAAGCTTGT+AGG | + | chr4.3:41489577-41489596 | MS.gene65295:CDS | 35.0% |
| !! | TTCTAAAGCTTGTAGGAAGT+TGG | + | chr4.3:41489584-41489603 | MS.gene65295:CDS | 35.0% |
| AAGAACATATCAGCAGTCTC+AGG | - | chr4.3:41489630-41489649 | None:intergenic | 40.0% | |
| AGTGGCTAGTGATGAGTATT+GGG | + | chr4.3:41489536-41489555 | MS.gene65295:CDS | 40.0% | |
| GGAAGAAGGGTTATGAATAG+AGG | + | chr4.3:41489465-41489484 | MS.gene65295:CDS | 40.0% | |
| !! | GCTTATGTTGTGACGGATAA+AGG | + | chr4.3:41489438-41489457 | MS.gene65295:CDS | 40.0% |
| !! | TTTCATTAGCCGGTTTTGCA+GGG | + | chr4.3:41489328-41489347 | MS.gene65295:CDS | 40.0% |
| AAGCTTGTAGGAAGTTGGCT+AGG | + | chr4.3:41489589-41489608 | MS.gene65295:CDS | 45.0% | |
| GAGTGGCTAGTGATGAGTAT+TGG | + | chr4.3:41489535-41489554 | MS.gene65295:CDS | 45.0% | |
| GGATAAAGGGTCTGGAAGAA+GGG | + | chr4.3:41489452-41489471 | MS.gene65295:CDS | 45.0% | |
| ! | GCAGGATTATTCTGGTTGGT+TGG | + | chr4.3:41489506-41489525 | MS.gene65295:CDS | 45.0% |
| ! | GTGGCTAGTGATGAGTATTG+GGG | + | chr4.3:41489537-41489556 | MS.gene65295:CDS | 45.0% |
| !! | GTTTCATTAGCCGGTTTTGC+AGG | + | chr4.3:41489327-41489346 | MS.gene65295:CDS | 45.0% |
| !! | TGGCTAGTGATGAGTATTGG+GGG | + | chr4.3:41489538-41489557 | MS.gene65295:CDS | 45.0% |
| !! | TTCATTAGCCGGTTTTGCAG+GGG | + | chr4.3:41489329-41489348 | MS.gene65295:CDS | 45.0% |
| CGGATAAAGGGTCTGGAAGA+AGG | + | chr4.3:41489451-41489470 | MS.gene65295:CDS | 50.0% | |
| ! | TGGTTGGTTGGAAGAGAGAG+TGG | + | chr4.3:41489518-41489537 | MS.gene65295:CDS | 50.0% |
| !! | GTTGTGACGGATAAAGGGTC+TGG | + | chr4.3:41489444-41489463 | MS.gene65295:CDS | 50.0% |
| GTAACACGCCCCTGCAAAAC+CGG | - | chr4.3:41489340-41489359 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 41489321 | 41489674 | 41489321 | ID=MS.gene65295 |
| chr4.3 | mRNA | 41489321 | 41489674 | 41489321 | ID=MS.gene65295.t1;Parent=MS.gene65295 |
| chr4.3 | exon | 41489321 | 41489674 | 41489321 | ID=MS.gene65295.t1.exon1;Parent=MS.gene65295.t1 |
| chr4.3 | CDS | 41489321 | 41489674 | 41489321 | ID=cds.MS.gene65295.t1;Parent=MS.gene65295.t1 |
| Gene Sequence |
| Protein sequence |
