Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74971.t1 | XP_013470277.1 | 90.4 | 114 | 11 | 0 | 1 | 114 | 183 | 296 | 7.50E-52 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74971.t1 | A0A072W228 | 90.4 | 114 | 11 | 0 | 1 | 114 | 183 | 296 | 5.4e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene74971 | 0.806983 | 6.31E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene74971 | 0.804001 | 2.66E-49 | -1.69E-46 |
| MS.gene050303 | MS.gene74971 | 0.835034 | 2.12E-56 | -1.69E-46 |
| MS.gene050438 | MS.gene74971 | 0.802575 | 5.26E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene74971 | 0.806732 | 7.13E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene74971 | 0.822839 | 1.91E-53 | -1.69E-46 |
| MS.gene050815 | MS.gene74971 | 0.822687 | 2.07E-53 | -1.69E-46 |
| MS.gene050844 | MS.gene74971 | 0.807122 | 5.90E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene74971 | 0.818932 | 1.51E-52 | -1.69E-46 |
| MS.gene051265 | MS.gene74971 | 0.835374 | 1.74E-56 | -1.69E-46 |
| MS.gene051344 | MS.gene74971 | 0.820344 | 7.21E-53 | -1.69E-46 |
| MS.gene052012 | MS.gene74971 | 0.801855 | 7.39E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene74971 | 0.841549 | 4.44E-58 | -1.69E-46 |
| MS.gene05289 | MS.gene74971 | 0.822121 | 2.81E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene74971 | 0.808718 | 2.70E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene74971 | 0.831052 | 2.08E-55 | -1.69E-46 |
| MS.gene053184 | MS.gene74971 | 0.801607 | 8.31E-49 | -1.69E-46 |
| MS.gene054184 | MS.gene74971 | 0.804656 | 1.95E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene74971 | 0.806203 | 9.22E-50 | -1.69E-46 |
| MS.gene054405 | MS.gene74971 | 0.814398 | 1.57E-51 | -1.69E-46 |
| MS.gene054433 | MS.gene74971 | 0.805372 | 1.38E-49 | -1.69E-46 |
| MS.gene054658 | MS.gene74971 | 0.824956 | 6.09E-54 | -1.69E-46 |
| MS.gene054713 | MS.gene74971 | 0.883975 | 2.84E-71 | -1.69E-46 |
| MS.gene055356 | MS.gene74971 | 0.822853 | 1.90E-53 | -1.69E-46 |
| MS.gene055357 | MS.gene74971 | 0.813158 | 2.95E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene74971 | 0.826061 | 3.34E-54 | -1.69E-46 |
| MS.gene055608 | MS.gene74971 | 0.853566 | 2.19E-61 | -1.69E-46 |
| MS.gene055818 | MS.gene74971 | 0.805722 | 1.16E-49 | -1.69E-46 |
| MS.gene055860 | MS.gene74971 | 0.829054 | 6.38E-55 | -1.69E-46 |
| MS.gene056498 | MS.gene74971 | 0.804933 | 1.70E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene74971 | 0.805246 | 1.46E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene74971 | 0.826512 | 2.60E-54 | -1.69E-46 |
| MS.gene058042 | MS.gene74971 | 0.85735 | 1.73E-62 | -1.69E-46 |
| MS.gene058168 | MS.gene74971 | 0.841865 | 3.67E-58 | -1.69E-46 |
| MS.gene059006 | MS.gene74971 | 0.806657 | 7.40E-50 | -1.69E-46 |
| MS.gene059609 | MS.gene74971 | 0.802965 | 4.37E-49 | -1.69E-46 |
| MS.gene05979 | MS.gene74971 | 0.815089 | 1.11E-51 | -1.69E-46 |
| MS.gene060137 | MS.gene74971 | -0.810943 | 8.97E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene74971 | 0.810869 | 9.30E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene74971 | 0.833928 | 4.02E-56 | -1.69E-46 |
| MS.gene060572 | MS.gene74971 | 0.812103 | 5.02E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene74971 | 0.825401 | 4.78E-54 | -1.69E-46 |
| MS.gene061114 | MS.gene74971 | -0.824093 | 9.73E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74971.t1 | MTR_1g112150 | 90.351 | 114 | 11 | 0 | 1 | 114 | 183 | 296 | 1.51e-73 | 221 |
| MS.gene74971.t1 | MTR_1g112160 | 65.254 | 118 | 41 | 0 | 1 | 118 | 134 | 251 | 1.17e-50 | 160 |
| MS.gene74971.t1 | MTR_2g021690 | 46.154 | 104 | 55 | 1 | 3 | 105 | 188 | 291 | 3.44e-21 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74971.t1 | AT1G55230 | 43.810 | 105 | 58 | 1 | 1 | 105 | 188 | 291 | 4.27e-26 | 99.0 |
| MS.gene74971.t1 | AT1G55240 | 41.748 | 103 | 60 | 0 | 3 | 105 | 190 | 292 | 6.14e-26 | 99.0 |
| MS.gene74971.t1 | AT5G19870 | 47.727 | 88 | 46 | 0 | 3 | 90 | 189 | 276 | 3.16e-24 | 93.6 |
Find 24 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATTATGGCTATAATTTA+TGG | 0.111898 | 1.3:+76026398 | MS.gene74971:CDS |
| CAATTCAGCTGGTTCTTTAT+TGG | 0.239215 | 1.3:+76026347 | MS.gene74971:CDS |
| GGTTTAGTGCTTTCTATATC+TGG | 0.242800 | 1.3:+76026221 | MS.gene74971:CDS |
| TCAACCCAAAGGTTGCTTCT+TGG | 0.301483 | 1.3:+76026247 | MS.gene74971:CDS |
| AGGTTTGTTCATTATGTTCA+TGG | 0.326708 | 1.3:+76026199 | MS.gene74971:CDS |
| AGTGTCTTTCTACTTGATTA+TGG | 0.354291 | 1.3:+76026385 | MS.gene74971:CDS |
| ATATTCCAAGAAGCAACCTT+TGG | 0.390404 | 1.3:-76026252 | None:intergenic |
| AAGGAGATCAACTTATGATT+AGG | 0.413259 | 1.3:+76026276 | MS.gene74971:CDS |
| CCAGATGATTTCTACTAGAA+TGG | 0.432826 | 1.3:+76026508 | MS.gene74971:CDS |
| TGGTGAATCTTCAATTCAGC+TGG | 0.442803 | 1.3:+76026336 | MS.gene74971:CDS |
| CCATTCTAGTAGAAATCATC+TGG | 0.450188 | 1.3:-76026508 | None:intergenic |
| GGTTTGTTCATTATGTTCAT+GGG | 0.458171 | 1.3:+76026200 | MS.gene74971:CDS |
| TATTCCAAGAAGCAACCTTT+GGG | 0.461299 | 1.3:-76026251 | None:intergenic |
| TATGCATCGTGCTGTTTCAT+TGG | 0.468772 | 1.3:+76026316 | MS.gene74971:CDS |
| GATTAGGTGCAATGATGAAA+AGG | 0.487514 | 1.3:+76026292 | MS.gene74971:CDS |
| GGTTGCTTCTTGGAATATGA+AGG | 0.488311 | 1.3:+76026257 | MS.gene74971:CDS |
| TGAACATAATGAACAAACCT+TGG | 0.523714 | 1.3:-76026196 | None:intergenic |
| TCTGTTAGCATGATCTTCCA+AGG | 0.532759 | 1.3:+76026179 | None:intergenic |
| GGAGTGCGATTCACTGATGA+AGG | 0.536386 | 1.3:+76026430 | MS.gene74971:CDS |
| AAGGAGGAACAGTACGGTGA+AGG | 0.576386 | 1.3:+76026449 | MS.gene74971:CDS |
| GAACAGTACGGTGAAGGCGA+TGG | 0.598192 | 1.3:+76026455 | MS.gene74971:CDS |
| ATATCTGGTTATCAACCCAA+AGG | 0.602519 | 1.3:+76026236 | MS.gene74971:CDS |
| GTGCGATTCACTGATGAAGG+AGG | 0.666959 | 1.3:+76026433 | MS.gene74971:CDS |
| CTGATGAAGGAGGAACAGTA+CGG | 0.675878 | 1.3:+76026443 | MS.gene74971:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAATTTATGGTGAAAAAG+TGG | + | chr1.3:76026409-76026428 | MS.gene74971:CDS | 20.0% |
| !! | TTGATTATGGCTATAATTTA+TGG | + | chr1.3:76026398-76026417 | MS.gene74971:CDS | 20.0% |
| AAGGAGATCAACTTATGATT+AGG | + | chr1.3:76026276-76026295 | MS.gene74971:CDS | 30.0% | |
| AGTGTCTTTCTACTTGATTA+TGG | + | chr1.3:76026385-76026404 | MS.gene74971:CDS | 30.0% | |
| TGAACATAATGAACAAACCT+TGG | - | chr1.3:76026199-76026218 | None:intergenic | 30.0% | |
| ! | AGGTTTGTTCATTATGTTCA+TGG | + | chr1.3:76026199-76026218 | MS.gene74971:CDS | 30.0% |
| ! | GGTTTGTTCATTATGTTCAT+GGG | + | chr1.3:76026200-76026219 | MS.gene74971:CDS | 30.0% |
| ATATCTGGTTATCAACCCAA+AGG | + | chr1.3:76026236-76026255 | MS.gene74971:CDS | 35.0% | |
| ATATTCCAAGAAGCAACCTT+TGG | - | chr1.3:76026255-76026274 | None:intergenic | 35.0% | |
| CCAGATGATTTCTACTAGAA+TGG | + | chr1.3:76026508-76026527 | MS.gene74971:CDS | 35.0% | |
| CCATTCTAGTAGAAATCATC+TGG | - | chr1.3:76026511-76026530 | None:intergenic | 35.0% | |
| GATTAGGTGCAATGATGAAA+AGG | + | chr1.3:76026292-76026311 | MS.gene74971:CDS | 35.0% | |
| GGTTTAGTGCTTTCTATATC+TGG | + | chr1.3:76026221-76026240 | MS.gene74971:CDS | 35.0% | |
| TATTCCAAGAAGCAACCTTT+GGG | - | chr1.3:76026254-76026273 | None:intergenic | 35.0% | |
| ! | CAATTCAGCTGGTTCTTTAT+TGG | + | chr1.3:76026347-76026366 | MS.gene74971:CDS | 35.0% |
| GGTTGCTTCTTGGAATATGA+AGG | + | chr1.3:76026257-76026276 | MS.gene74971:CDS | 40.0% | |
| TGGTGAATCTTCAATTCAGC+TGG | + | chr1.3:76026336-76026355 | MS.gene74971:CDS | 40.0% | |
| !! | TATGCATCGTGCTGTTTCAT+TGG | + | chr1.3:76026316-76026335 | MS.gene74971:CDS | 40.0% |
| CTGATGAAGGAGGAACAGTA+CGG | + | chr1.3:76026443-76026462 | MS.gene74971:CDS | 45.0% | |
| TCAACCCAAAGGTTGCTTCT+TGG | + | chr1.3:76026247-76026266 | MS.gene74971:CDS | 45.0% | |
| AAGGAGGAACAGTACGGTGA+AGG | + | chr1.3:76026449-76026468 | MS.gene74971:CDS | 50.0% | |
| GGAGTGCGATTCACTGATGA+AGG | + | chr1.3:76026430-76026449 | MS.gene74971:CDS | 50.0% | |
| GTGCGATTCACTGATGAAGG+AGG | + | chr1.3:76026433-76026452 | MS.gene74971:CDS | 50.0% | |
| GAACAGTACGGTGAAGGCGA+TGG | + | chr1.3:76026455-76026474 | MS.gene74971:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 76026188 | 76026544 | 76026188 | ID=MS.gene74971 |
| chr1.3 | mRNA | 76026188 | 76026544 | 76026188 | ID=MS.gene74971.t1;Parent=MS.gene74971 |
| chr1.3 | exon | 76026188 | 76026544 | 76026188 | ID=MS.gene74971.t1.exon1;Parent=MS.gene74971.t1 |
| chr1.3 | CDS | 76026188 | 76026544 | 76026188 | ID=cds.MS.gene74971.t1;Parent=MS.gene74971.t1 |
| Gene Sequence |
| Protein sequence |
