AlfalfaGEDB | MS.gene75113

M. sativa cultivar XinJiangDaYe / MS.gene75113

KEGG: -
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75113.t1	RHN52993.1	74.5	106	22	1	1	101	1	106	2.10E-34	154.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75113.t1	G7KPU6	74.5	106	22	1	1	101	1	106	1.5e-34	154.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene75113	0.826887	2.12E-54	-1.69E-46
MS.gene049254	MS.gene75113	0.812903	3.35E-51	-1.69E-46
MS.gene049283	MS.gene75113	0.821729	3.46E-53	-1.69E-46
MS.gene049350	MS.gene75113	0.812159	4.88E-51	-1.69E-46
MS.gene049368	MS.gene75113	0.818987	1.47E-52	-1.69E-46
MS.gene049419	MS.gene75113	-0.803012	4.27E-49	-1.69E-46
MS.gene049499	MS.gene75113	0.805785	1.13E-49	-1.69E-46
MS.gene049983	MS.gene75113	0.827807	1.28E-54	-1.69E-46
MS.gene050414	MS.gene75113	0.830094	3.56E-55	-1.69E-46
MS.gene050646	MS.gene75113	0.816809	4.57E-52	-1.69E-46
MS.gene050654	MS.gene75113	0.825326	4.98E-54	-1.69E-46
MS.gene051343	MS.gene75113	0.8064	8.38E-50	-1.69E-46
MS.gene051676	MS.gene75113	0.803311	3.70E-49	-1.69E-46
MS.gene051856	MS.gene75113	0.805834	1.10E-49	-1.69E-46
MS.gene051902	MS.gene75113	0.806816	6.84E-50	-1.69E-46
MS.gene052016	MS.gene75113	0.821714	3.49E-53	-1.69E-46
MS.gene052242	MS.gene75113	-0.800383	1.48E-48	-1.69E-46
MS.gene052307	MS.gene75113	0.825888	3.67E-54	-1.69E-46
MS.gene052463	MS.gene75113	0.84086	6.74E-58	-1.69E-46
MS.gene052609	MS.gene75113	0.825166	5.44E-54	-1.69E-46
MS.gene052932	MS.gene75113	0.821827	3.28E-53	-1.69E-46
MS.gene053079	MS.gene75113	0.80806	3.73E-50	-1.69E-46
MS.gene05364	MS.gene75113	0.800418	1.45E-48	-1.69E-46
MS.gene054479	MS.gene75113	0.820325	7.28E-53	-1.69E-46
MS.gene05458	MS.gene75113	0.807325	5.34E-50	-1.69E-46
MS.gene054709	MS.gene75113	0.800202	1.61E-48	-1.69E-46
MS.gene054892	MS.gene75113	0.807104	5.95E-50	-1.69E-46
MS.gene054893	MS.gene75113	0.807894	4.04E-50	-1.69E-46
MS.gene055313	MS.gene75113	0.818016	2.44E-52	-1.69E-46
MS.gene055513	MS.gene75113	0.849603	2.91E-60	-1.69E-46
MS.gene055864	MS.gene75113	0.8012	1.01E-48	-1.69E-46
MS.gene056045	MS.gene75113	0.842054	3.27E-58	-1.69E-46
MS.gene056308	MS.gene75113	0.826029	3.39E-54	-1.69E-46
MS.gene056578	MS.gene75113	0.804939	1.70E-49	-1.69E-46
MS.gene056699	MS.gene75113	0.800658	1.30E-48	-1.69E-46
MS.gene056776	MS.gene75113	0.812944	3.29E-51	-1.69E-46
MS.gene057034	MS.gene75113	0.818204	2.21E-52	-1.69E-46
MS.gene057102	MS.gene75113	0.818152	2.28E-52	-1.69E-46
MS.gene057155	MS.gene75113	0.814179	1.76E-51	-1.69E-46
MS.gene057208	MS.gene75113	0.825334	4.96E-54	-1.69E-46
MS.gene057371	MS.gene75113	0.802082	6.64E-49	-1.69E-46
MS.gene058081	MS.gene75113	0.800486	1.41E-48	-1.69E-46
MS.gene058330	MS.gene75113	0.81498	1.17E-51	-1.69E-46
MS.gene059439	MS.gene75113	0.813624	2.33E-51	-1.69E-46
MS.gene05985	MS.gene75113	-0.809002	2.35E-50	-1.69E-46
MS.gene059965	MS.gene75113	0.812115	4.99E-51	-1.69E-46
MS.gene060365	MS.gene75113	0.825311	5.02E-54	-1.69E-46
MS.gene060777	MS.gene75113	0.841703	4.05E-58	-1.69E-46
MS.gene060932	MS.gene75113	0.827755	1.31E-54	-1.69E-46
MS.gene060933	MS.gene75113	0.81903	1.44E-52	-1.69E-46
MS.gene060935	MS.gene75113	0.821347	4.24E-53	-1.69E-46
MS.gene060937	MS.gene75113	0.835071	2.08E-56	-1.69E-46
MS.gene061100	MS.gene75113	0.838773	2.36E-57	-1.69E-46
MS.gene061188	MS.gene75113	0.802409	5.69E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75113.t1	MTR_6g088340	74.528	106	22	1	1	101	1	106	1.18e-47	157
MS.gene75113.t1	MTR_6g092140	79.048	105	9	4	1	101	1	96	1.21e-46	155
MS.gene75113.t1	MTR_6g091830	79.048	105	9	4	1	101	1	96	2.33e-46	154

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 22 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGCGTAATCGTTTAATTT+TGG	0.114594	6.3:+84405901	MS.gene75113:CDS
CCAGTACTAAAATCTGAAAT+TGG	0.128271	6.3:+84405719	MS.gene75113:CDS
AGGATGAATAAGGTACTTAA+AGG	0.337066	6.3:+84405881	MS.gene75113:CDS
TTTCAAAGAAAGGATGAATA+AGG	0.348934	6.3:+84405871	MS.gene75113:CDS
TGACCACCTCGTTGTCGAAT+TGG	0.360565	6.3:-84405962	None:intergenic
CAGTACTAAAATCTGAAATT+GGG	0.378228	6.3:+84405720	MS.gene75113:CDS
CAGTTCAAAATTTCAAAGAA+AGG	0.407992	6.3:+84405861	MS.gene75113:CDS
AAAATCTGAAATTGGGTTTG+TGG	0.415681	6.3:+84405727	MS.gene75113:CDS
TGATTCGATGATCATTCATC+AGG	0.445423	6.3:-84405829	None:intergenic
AATCTGTGTTTCAAGGTAAG+AGG	0.459600	6.3:+84405759	MS.gene75113:CDS
GGATGAATAAGGTACTTAAA+GGG	0.501407	6.3:+84405882	MS.gene75113:CDS
ATCTGTGTTTCAAGGTAAGA+GGG	0.522269	6.3:+84405760	MS.gene75113:CDS
GATTCCATGTTCGTTTGCCA+TGG	0.525485	6.3:-84405690	None:intergenic
GCCTCCATGGCAAACGAACA+TGG	0.527907	6.3:+84405686	None:intergenic
GAAGTAAAATCTGTGTTTCA+AGG	0.545805	6.3:+84405752	MS.gene75113:CDS
CAACGAGGTGGTCATAGTAA+TGG	0.556109	6.3:+84405971	MS.gene75113:CDS
ATCTGAAATTGGGTTTGTGG+AGG	0.559459	6.3:+84405730	MS.gene75113:CDS
AATACGACGATGCTTCATGT+AGG	0.643363	6.3:-84405933	None:intergenic
GATTCGATGATCATTCATCA+GGG	0.648898	6.3:-84405828	None:intergenic
CTTCCAATTCGACAACGAGG+TGG	0.719023	6.3:+84405959	MS.gene75113:CDS
TCGATGATCATTCATCAGGG+TGG	0.722997	6.3:-84405825	None:intergenic
ACACTTCCAATTCGACAACG+AGG	0.767405	6.3:+84405956	MS.gene75113:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAGTTCAAAATTTCAAAGAA+AGG	+	chr6.3:84405861-84405880	MS.gene75113:CDS	25.0%
!	TTTCAAAGAAAGGATGAATA+AGG	+	chr6.3:84405871-84405890	MS.gene75113:CDS	25.0%
!!	CAGTACTAAAATCTGAAATT+GGG	+	chr6.3:84405720-84405739	MS.gene75113:CDS	25.0%
!!!	CTTTCTTTGAAATTTTGAAC+TGG	-	chr6.3:84405863-84405882	None:intergenic	25.0%
	GAAGTAAAATCTGTGTTTCA+AGG	+	chr6.3:84405752-84405771	MS.gene75113:CDS	30.0%
!	AAAATCTGAAATTGGGTTTG+TGG	+	chr6.3:84405727-84405746	MS.gene75113:CDS	30.0%
!	AGGATGAATAAGGTACTTAA+AGG	+	chr6.3:84405881-84405900	MS.gene75113:CDS	30.0%
!	CCAGTACTAAAATCTGAAAT+TGG	+	chr6.3:84405719-84405738	MS.gene75113:CDS	30.0%
!	GGATGAATAAGGTACTTAAA+GGG	+	chr6.3:84405882-84405901	MS.gene75113:CDS	30.0%
!!	CCAATTTCAGATTTTAGTAC+TGG	-	chr6.3:84405722-84405741	None:intergenic	30.0%
	AATCTGTGTTTCAAGGTAAG+AGG	+	chr6.3:84405759-84405778	MS.gene75113:CDS	35.0%
	ATCTGTGTTTCAAGGTAAGA+GGG	+	chr6.3:84405760-84405779	MS.gene75113:CDS	35.0%
	GATTCGATGATCATTCATCA+GGG	-	chr6.3:84405831-84405850	None:intergenic	35.0%
	TGATTCGATGATCATTCATC+AGG	-	chr6.3:84405832-84405851	None:intergenic	35.0%
!	GAAATTTTGAACTGGAAGTC+TGG	-	chr6.3:84405855-84405874	None:intergenic	35.0%
!!	AGGGCGTAATCGTTTAATTT+TGG	+	chr6.3:84405901-84405920	MS.gene75113:CDS	35.0%
!!	GGGCGTAATCGTTTAATTTT+GGG	+	chr6.3:84405902-84405921	MS.gene75113:CDS	35.0%
	AATACGACGATGCTTCATGT+AGG	-	chr6.3:84405936-84405955	None:intergenic	40.0%
!	ATCTGAAATTGGGTTTGTGG+AGG	+	chr6.3:84405730-84405749	MS.gene75113:CDS	40.0%
	ACACTTCCAATTCGACAACG+AGG	+	chr6.3:84405956-84405975	MS.gene75113:CDS	45.0%
	CAACGAGGTGGTCATAGTAA+TGG	+	chr6.3:84405971-84405990	MS.gene75113:CDS	45.0%
	TCGATGATCATTCATCAGGG+TGG	-	chr6.3:84405828-84405847	None:intergenic	45.0%
	CTTCCAATTCGACAACGAGG+TGG	+	chr6.3:84405959-84405978	MS.gene75113:CDS	50.0%
	TGACCACCTCGTTGTCGAAT+TGG	-	chr6.3:84405965-84405984	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	84405692	84405997	84405692	ID=MS.gene75113
chr6.3	mRNA	84405692	84405997	84405692	ID=MS.gene75113.t1;Parent=MS.gene75113
chr6.3	exon	84405692	84405997	84405692	ID=MS.gene75113.t1.exon1;Parent=MS.gene75113.t1
chr6.3	CDS	84405692	84405997	84405692	ID=cds.MS.gene75113.t1;Parent=MS.gene75113.t1

Gene Sequence	>MS.gene75113 ATGGCAAACGAACATGGAATCACACTTCCAGTACTAAAATCTGAAATTGGGTTTGTGGAGGAAGTAAAATCTGTGTTTCAAGGTAAGAGGGAAGAGTATGAAGAGTTTATAAAAGACATACATGCTTTTAAGACCACCCTGATGAATGATCATCGAATCACCAGACTTCCAGTTCAAAATTTCAAAGAAAGGATGAATAAGGTACTTAAAGGGCGTAATCGTTTAATTTTGGGATTCAACGCCTACATGAAGCATCGTCGTATTACACTTCCAATTCGACAACGAGGTGGTCATAGTAATGGTTAG
Protein sequence	>MS.gene75113.t1 MANEHGITLPVLKSEIGFVEEVKSVFQGKREEYEEFIKDIHAFKTTLMNDHRITRLPVQNFKERMNKVLKGRNRLILGFNAYMKHRRITLPIRQRGGHSNG