AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79094


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79094.t1 MTR_3g086830 94.156 154 9 0 1 154 1 154 1.32e-81 238
MS.gene79094.t1 MTR_8g089920 43.137 153 75 4 4 154 1 143 6.05e-33 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGGTAATTGTTTATCATT+TGG 0.298328 3.2:-74147421 None:intergenic
AGGAATCTGTAGTGAATTCT+AGG 0.331571 3.2:+74147800 MS.gene79094:CDS
AGGGATGTTGGTGGAGGTAG+AGG 0.333746 3.2:+74147721 MS.gene79094:CDS
GAAGAAAGAAAGATGGCTTT+TGG 0.373560 3.2:+74147743 MS.gene79094:CDS
TTGTTTCGAGGAACCGAGTC+AGG 0.374583 3.2:+74147695 MS.gene79094:CDS
AGGTACTCAAATTGAGGAAA+AGG 0.393008 3.2:+74147770 MS.gene79094:CDS
GAGAGAATCAATAGGGTTCT+TGG 0.399060 3.2:-74147488 None:intergenic
TGAGGAGAAGCAGGAGAAGA+AGG 0.411004 3.2:-74147523 None:intergenic
CAATAGTAGAGAGAATCAAT+AGG 0.415959 3.2:-74147496 None:intergenic
TTTGTTGAGGCATCATCAAT+AGG 0.416519 3.2:-74147547 None:intergenic
GATGATGCCTCAACAAATCA+TGG 0.439486 3.2:+74147553 MS.gene79094:CDS
TAGTGAATTCTAGGACTTGA+AGG 0.449666 3.2:+74147809 MS.gene79094:CDS
GGTCCAAGGTACTCAAATTG+AGG 0.456858 3.2:+74147764 MS.gene79094:CDS
TTTCCTCAATTTGAGTACCT+TGG 0.458595 3.2:-74147767 None:intergenic
AGGGAGAGGGATGTTGGTGG+AGG 0.478556 3.2:+74147715 MS.gene79094:CDS
AGTCGCTTGCCGTTGTTTCG+AGG 0.489139 3.2:+74147683 MS.gene79094:CDS
TCAATAGGTTGAGGAGAAGC+AGG 0.490038 3.2:-74147532 None:intergenic
GGTACTCAAATTGAGGAAAA+GGG 0.507988 3.2:+74147771 MS.gene79094:CDS
TGTCATACAAGAAGCAGAAC+CGG 0.515419 3.2:-74147626 None:intergenic
AATTAGAAGCAACAAGAGTA+AGG 0.516499 3.2:+74147386 MS.gene79094:CDS
GGTAGAGGAAGAAAGAAAGA+TGG 0.520786 3.2:+74147736 MS.gene79094:CDS
CGAGGAACCGAGTCAGGGAG+AGG 0.521049 3.2:+74147701 MS.gene79094:CDS
AATAGTAGAGAGAATCAATA+GGG 0.528508 3.2:-74147495 None:intergenic
CAACATCCCTCTCCCTGACT+CGG 0.533206 3.2:-74147708 None:intergenic
CGAGTCAGGGAGAGGGATGT+TGG 0.536071 3.2:+74147709 MS.gene79094:CDS
CAATAATGAATCAGATGAAG+TGG 0.549086 3.2:+74147359 None:intergenic
GTCAGGGAGAGGGATGTTGG+TGG 0.559065 3.2:+74147712 MS.gene79094:CDS
GAGGCATCATCAATAGGTTG+AGG 0.568712 3.2:-74147541 None:intergenic
GAGTTTGTCTCTTAAACAAG+AGG 0.578724 3.2:-74147452 None:intergenic
CCAAATGATAAACAATTACC+AGG 0.580717 3.2:+74147421 MS.gene79094:CDS
GAGGAACCGAGTCAGGGAGA+GGG 0.581827 3.2:+74147702 MS.gene79094:CDS
ATCGTAGTAGACACTTTCGC+AGG 0.593355 3.2:+74147587 MS.gene79094:CDS
TGAAGACCCATGATTTGTTG+AGG 0.607126 3.2:-74147560 None:intergenic
AGGAATGCTTCACAAGCGAC+CGG 0.610619 3.2:+74147607 MS.gene79094:CDS
ATGATGCCTCAACAAATCAT+GGG 0.612329 3.2:+74147554 MS.gene79094:CDS
ATTGAGGAAAAGGGATACAG+AGG 0.620071 3.2:+74147780 MS.gene79094:CDS
TGTTTCGAGGAACCGAGTCA+GGG 0.624425 3.2:+74147696 MS.gene79094:CDS
AAACAAGAGGAACAAACACC+TGG 0.650993 3.2:-74147439 None:intergenic
GACTCGGTTCCTCGAAACAA+CGG 0.676399 3.2:-74147692 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATAGTAGAGAGAATCAATA+GGG - chr3.2:74147498-74147517 None:intergenic 25.0%
AATTAGAAGCAACAAGAGTA+AGG + chr3.2:74147386-74147405 MS.gene79094:CDS 30.0%
CAATAGTAGAGAGAATCAAT+AGG - chr3.2:74147499-74147518 None:intergenic 30.0%
CCAAATGATAAACAATTACC+AGG + chr3.2:74147421-74147440 MS.gene79094:CDS 30.0%
CCTGGTAATTGTTTATCATT+TGG - chr3.2:74147424-74147443 None:intergenic 30.0%
AGGAATCTGTAGTGAATTCT+AGG + chr3.2:74147800-74147819 MS.gene79094:CDS 35.0%
AGGTACTCAAATTGAGGAAA+AGG + chr3.2:74147770-74147789 MS.gene79094:CDS 35.0%
ATGATGCCTCAACAAATCAT+GGG + chr3.2:74147554-74147573 MS.gene79094:CDS 35.0%
GAGTTTGTCTCTTAAACAAG+AGG - chr3.2:74147455-74147474 None:intergenic 35.0%
GGTACTCAAATTGAGGAAAA+GGG + chr3.2:74147771-74147790 MS.gene79094:CDS 35.0%
TTTCCTCAATTTGAGTACCT+TGG - chr3.2:74147770-74147789 None:intergenic 35.0%
! GAAGAAAGAAAGATGGCTTT+TGG + chr3.2:74147743-74147762 MS.gene79094:CDS 35.0%
! TTTGTTGAGGCATCATCAAT+AGG - chr3.2:74147550-74147569 None:intergenic 35.0%
AAACAAGAGGAACAAACACC+TGG - chr3.2:74147442-74147461 None:intergenic 40.0%
ATTGAGGAAAAGGGATACAG+AGG + chr3.2:74147780-74147799 MS.gene79094:CDS 40.0%
GAGAGAATCAATAGGGTTCT+TGG - chr3.2:74147491-74147510 None:intergenic 40.0%
GATGATGCCTCAACAAATCA+TGG + chr3.2:74147553-74147572 MS.gene79094:CDS 40.0%
GGTAGAGGAAGAAAGAAAGA+TGG + chr3.2:74147736-74147755 MS.gene79094:CDS 40.0%
TGTCATACAAGAAGCAGAAC+CGG - chr3.2:74147629-74147648 None:intergenic 40.0%
!! TGAAGACCCATGATTTGTTG+AGG - chr3.2:74147563-74147582 None:intergenic 40.0%
ATCGTAGTAGACACTTTCGC+AGG + chr3.2:74147587-74147606 MS.gene79094:CDS 45.0%
! GAAAGATGGCTTTTGGTCCA+AGG + chr3.2:74147750-74147769 MS.gene79094:CDS 45.0%
! GAGGCATCATCAATAGGTTG+AGG - chr3.2:74147544-74147563 None:intergenic 45.0%
! GGTCCAAGGTACTCAAATTG+AGG + chr3.2:74147764-74147783 MS.gene79094:CDS 45.0%
! TCAATAGGTTGAGGAGAAGC+AGG - chr3.2:74147535-74147554 None:intergenic 45.0%
AGGAATGCTTCACAAGCGAC+CGG + chr3.2:74147607-74147626 MS.gene79094:CDS 50.0%
TGAGGAGAAGCAGGAGAAGA+AGG - chr3.2:74147526-74147545 None:intergenic 50.0%
TGTTTCGAGGAACCGAGTCA+GGG + chr3.2:74147696-74147715 MS.gene79094:CDS 50.0%
TTGTTTCGAGGAACCGAGTC+AGG + chr3.2:74147695-74147714 MS.gene79094:CDS 50.0%
! GACTCGGTTCCTCGAAACAA+CGG - chr3.2:74147695-74147714 None:intergenic 50.0%
CAACATCCCTCTCCCTGACT+CGG - chr3.2:74147711-74147730 None:intergenic 55.0%
! AGGGATGTTGGTGGAGGTAG+AGG + chr3.2:74147721-74147740 MS.gene79094:CDS 55.0%
! AGTCGCTTGCCGTTGTTTCG+AGG + chr3.2:74147683-74147702 MS.gene79094:CDS 55.0%
CGAGTCAGGGAGAGGGATGT+TGG + chr3.2:74147709-74147728 MS.gene79094:CDS 60.0%
GAGGAACCGAGTCAGGGAGA+GGG + chr3.2:74147702-74147721 MS.gene79094:CDS 60.0%
GTCAGGGAGAGGGATGTTGG+TGG + chr3.2:74147712-74147731 MS.gene79094:CDS 60.0%
! AGGGAGAGGGATGTTGGTGG+AGG + chr3.2:74147715-74147734 MS.gene79094:CDS 60.0%
CGAGGAACCGAGTCAGGGAG+AGG + chr3.2:74147701-74147720 MS.gene79094:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 74147364 74147828 74147364 ID=MS.gene79094
chr3.2 mRNA 74147364 74147828 74147364 ID=MS.gene79094.t1;Parent=MS.gene79094
chr3.2 exon 74147364 74147828 74147364 ID=MS.gene79094.t1.exon1;Parent=MS.gene79094.t1
chr3.2 CDS 74147364 74147828 74147364 ID=cds.MS.gene79094.t1;Parent=MS.gene79094.t1
Gene Sequence

>MS.gene79094

ATGAATCAGATGAAGTGGTGCGAATTAGAAGCAACAAGAGTAAGGTGCAAAGAACATCCAAATGATAAACAATTACCAGGTGTTTGTTCCTCTTGTTTAAGAGACAAACTCTCAAATCTATACACCAAGAACCCTATTGATTCTCTCTACTATTGTTCTCCTTCTTCTCCTGCTTCTCCTCAACCTATTGATGATGCCTCAACAAATCATGGGTCTTCAGCTCATCGTAGTAGACACTTTCGCAGGAATGCTTCACAAGCGACCGGTTCTGCTTCTTGTATGACAAGCTTTAATCATGCGTTGAACTTGAAGAAGAGCAAGTCGCTTGCCGTTGTTTCGAGGAACCGAGTCAGGGAGAGGGATGTTGGTGGAGGTAGAGGAAGAAAGAAAGATGGCTTTTGGTCCAAGGTACTCAAATTGAGGAAAAGGGATACAGAGGAATCTGTAGTGAATTCTAGGACTTGA

Protein sequence

>MS.gene79094.t1

MNQMKWCELEATRVRCKEHPNDKQLPGVCSSCLRDKLSNLYTKNPIDSLYYCSPSSPASPQPIDDASTNHGSSAHRSRHFRRNASQATGSASCMTSFNHALNLKKSKSLAVVSRNRVRERDVGGGRGRKKDGFWSKVLKLRKRDTEESVVNSRT