AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84014


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84014.t1 MTR_1g050472 99.394 330 2 0 1 330 1 330 0.0 678
MS.gene84014.t1 MTR_0268s0080 96.970 330 10 0 1 330 1 330 0.0 667
MS.gene84014.t1 MTR_7g029110 96.061 330 13 0 1 330 1 330 0.0 641
MS.gene84014.t1 MTR_8g469010 92.727 330 19 1 1 330 1 325 0.0 634
MS.gene84014.t1 MTR_4g117370 76.308 325 76 1 6 330 18 341 0.0 532
MS.gene84014.t1 MTR_6g005270 67.442 43 14 0 265 307 25 67 1.63e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84014.t1 AT3G27060 83.735 332 52 2 1 330 1 332 0.0 558
MS.gene84014.t1 AT3G23580 76.308 325 76 1 6 330 18 341 0.0 518

Find 61 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGAATCGGTCTGGGTTT+GGG 0.237047 1.3:-32143902 None:intergenic
TGCGCATTGGTTGGGATGAA+TGG 0.293239 1.3:+32144605 MS.gene84014:CDS
GATGGAATTGATTTCGCTTC+AGG 0.300115 1.3:+32144724 MS.gene84014:CDS
CGCTTCCTTGCGCATTGGTT+GGG 0.302060 1.3:+32144597 MS.gene84014:CDS
GTTTGTTGCTGATAGGTTAT+TGG 0.303774 1.3:+32144652 MS.gene84014:CDS
AGTGGCGTTCGCCGTCTGTT+AGG 0.311051 1.3:-32144034 None:intergenic
GGGGCAAGAAGGGGTTCTTC+TGG 0.330658 1.3:-32143882 None:intergenic
ATGCAGAATCGGTCTGGGTT+TGG 0.338926 1.3:-32143903 None:intergenic
GCGCATTGGTTGGGATGAAT+GGG 0.350139 1.3:+32144606 MS.gene84014:CDS
AAGCTGATTGGGCGATGAAA+TGG 0.358474 1.3:+32144297 MS.gene84014:CDS
ACAATTTCTTTCACGCGCTC+TGG 0.374811 1.3:-32144533 None:intergenic
TTTGTTGCTGATAGGTTATT+GGG 0.379609 1.3:+32144653 MS.gene84014:CDS
AATCGCTTATATACGTTTCA+AGG 0.387103 1.3:-32144217 None:intergenic
ATGTTCAGAATCCGTTTGAT+TGG 0.390491 1.3:+32144702 MS.gene84014:CDS
ACCGTAGAAGGCACGTGCTT+CGG 0.402800 1.3:-32144147 None:intergenic
GTCTGGGTTTGGGGCAAGAA+GGG 0.415976 1.3:-32143892 None:intergenic
GGGAACATGCAGAATCGGTC+TGG 0.418807 1.3:-32143909 None:intergenic
GCGCTTCCTTGCGCATTGGT+TGG 0.429604 1.3:+32144596 MS.gene84014:CDS
GATTTCTTTCATGAATCTAC+CGG 0.435670 1.3:-32144117 None:intergenic
GTTGCATTTGCTTGTGTTGA+AGG 0.436600 1.3:+32144353 MS.gene84014:CDS
TCTGTTATGAATAGCTTGAA+TGG 0.443380 1.3:+32144794 MS.gene84014:CDS
TCAGAATCCGTTTGATTGGA+TGG 0.443597 1.3:+32144706 MS.gene84014:CDS
TCTGGGTTTGGGGCAAGAAG+GGG 0.444554 1.3:-32143891 None:intergenic
AGGCACGTGCTTCGGAGACT+TGG 0.444676 1.3:-32144139 None:intergenic
GCCGTCGGAGGCGGCGAAGA+AGG 0.452615 1.3:-32144075 None:intergenic
GAAAATCGCACAGAAACTTC+CGG 0.473049 1.3:-32144387 None:intergenic
GAGGACGATGCCGTCGGAGG+CGG 0.474359 1.3:-32144084 None:intergenic
TGCAATCTGGAAACCGTAGA+AGG 0.475411 1.3:-32144159 None:intergenic
ATCAATTCCATCCAATCAAA+CGG 0.480364 1.3:-32144713 None:intergenic
CGACGCGCTTCCTTGCGCAT+TGG 0.483293 1.3:+32144592 MS.gene84014:CDS
GAGCTTATTTCACGTGATGA+AGG 0.484978 1.3:+32144455 MS.gene84014:CDS
TCCGAAGCACGTGCCTTCTA+CGG 0.485174 1.3:+32144146 MS.gene84014:CDS
GATAGGTTATTGGGTGAGCT+TGG 0.486660 1.3:+32144662 MS.gene84014:CDS
GGAACATGCAGAATCGGTCT+GGG 0.489835 1.3:-32143908 None:intergenic
CGCATTGGTTGGGATGAATG+GGG 0.491773 1.3:+32144607 MS.gene84014:CDS
ATATTGAGTTTGTTGCTGAT+AGG 0.493329 1.3:+32144645 MS.gene84014:CDS
ATGGAATTGATTTCGCTTCA+GGG 0.495575 1.3:+32144725 MS.gene84014:CDS
GTGATGCTGTTGAAATTGAA+AGG 0.499878 1.3:+32144558 MS.gene84014:CDS
AGGGGTTCTTCTGGAAAAGC+AGG 0.505225 1.3:-32143873 None:intergenic
GGGTGAGCTTGGTTGTGAAA+AGG 0.505349 1.3:+32144673 MS.gene84014:CDS
GCCTTCTTCGCCGCCTCCGA+CGG 0.505987 1.3:+32144074 MS.gene84014:CDS
GGTCTGGGTTTGGGGCAAGA+AGG 0.509820 1.3:-32143893 None:intergenic
TTCTTCTGCGGTCCAGAATG+AGG 0.510696 1.3:-32143976 None:intergenic
GGATTGGGAACATGCAGAAT+CGG 0.521690 1.3:-32143914 None:intergenic
GCAGAATCGGTCTGGGTTTG+GGG 0.524980 1.3:-32143901 None:intergenic
TGAAATAAGCTCGTTTGAGA+AGG 0.538441 1.3:-32144444 None:intergenic
TGATGCTGTTGAAATTGAAA+GGG 0.545948 1.3:+32144559 MS.gene84014:CDS
ATGAATAGCTTGAATGGTAA+TGG 0.546534 1.3:+32144800 MS.gene84014:CDS
TTTCCTCCGATCTCAAGCAC+TGG 0.566756 1.3:+32144006 MS.gene84014:CDS
TTCGAGGACGATGCCGTCGG+AGG 0.583990 1.3:-32144087 None:intergenic
CGAGGACGTGAGAGATGAAG+TGG 0.584744 1.3:-32144052 None:intergenic
TCGTTTGAGAAGGTTAATCC+CGG 0.587906 1.3:-32144434 None:intergenic
CGGAGGCGGCGAAGAAGGCG+AGG 0.590500 1.3:-32144070 None:intergenic
TGGAATTGATTTCGCTTCAG+GGG 0.591747 1.3:+32144726 MS.gene84014:CDS
GCATTGGTTGGGATGAATGG+GGG 0.596821 1.3:+32144608 MS.gene84014:CDS
TTCATCCCAACCAATGCGCA+AGG 0.604485 1.3:-32144602 None:intergenic
CACTGGCAAAACCTAACAGA+CGG 0.606745 1.3:+32144023 MS.gene84014:CDS
TTGCCAGTGCTTGAGATCGG+AGG 0.610798 1.3:-32144009 None:intergenic
ATCGTCCTCGAAAATCTCGC+CGG 0.639774 1.3:+32144098 MS.gene84014:CDS
GGAAAGATCGACTTCTTCTG+CGG 0.673797 1.3:-32143988 None:intergenic
GAAGAGAGTACATCTCAGAG+TGG 0.694344 1.3:-32144193 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACACGCTTCTCAAAAAAAT+TGG - chr1.3:32144756-32144775 None:intergenic 30.0%
AATCCAATACCCAAAAATCT+GGG + chr1.3:32143932-32143951 MS.gene84014:CDS 30.0%
AATCGCTTATATACGTTTCA+AGG - chr1.3:32144220-32144239 None:intergenic 30.0%
ATCAATTCCATCCAATCAAA+CGG - chr1.3:32144716-32144735 None:intergenic 30.0%
ATGAATAGCTTGAATGGTAA+TGG + chr1.3:32144800-32144819 MS.gene84014:CDS 30.0%
GATTTCTTTCATGAATCTAC+CGG - chr1.3:32144120-32144139 None:intergenic 30.0%
TCTGTTATGAATAGCTTGAA+TGG + chr1.3:32144794-32144813 MS.gene84014:CDS 30.0%
TGATGCTGTTGAAATTGAAA+GGG + chr1.3:32144559-32144578 MS.gene84014:CDS 30.0%
TTTGTTGCTGATAGGTTATT+GGG + chr1.3:32144653-32144672 MS.gene84014:CDS 30.0%
! TGTACATTTCCCAGATTTTT+GGG - chr1.3:32143944-32143963 None:intergenic 30.0%
! TTGTACATTTCCCAGATTTT+TGG - chr1.3:32143945-32143964 None:intergenic 30.0%
!! ATATTGAGTTTGTTGCTGAT+AGG + chr1.3:32144645-32144664 MS.gene84014:CDS 30.0%
!!! AATTTTTTTGAGAAGCGTGT+TGG + chr1.3:32144755-32144774 MS.gene84014:CDS 30.0%
!!! AGATTTTTGGGTATTGGATT+GGG - chr1.3:32143932-32143951 None:intergenic 30.0%
!!! ATTTTCTGGTTGAAAAAACG+TGG + chr1.3:32144404-32144423 MS.gene84014:CDS 30.0%
!!! ATTTTTTTGAGAAGCGTGTT+GGG + chr1.3:32144756-32144775 MS.gene84014:CDS 30.0%
AAAAAACGTGGTTTAATGCC+GGG + chr1.3:32144416-32144435 MS.gene84014:CDS 35.0%
ATGTTCAGAATCCGTTTGAT+TGG + chr1.3:32144702-32144721 MS.gene84014:CDS 35.0%
CAATCCAATACCCAAAAATC+TGG + chr1.3:32143931-32143950 MS.gene84014:CDS 35.0%
GAAAAAACGTGGTTTAATGC+CGG + chr1.3:32144415-32144434 MS.gene84014:CDS 35.0%
GTGATGCTGTTGAAATTGAA+AGG + chr1.3:32144558-32144577 MS.gene84014:CDS 35.0%
TATCGCGAAAAAAGCTGATT+GGG + chr1.3:32144286-32144305 MS.gene84014:CDS 35.0%
TGAAATAAGCTCGTTTGAGA+AGG - chr1.3:32144447-32144466 None:intergenic 35.0%
! ATGGAATTGATTTCGCTTCA+GGG + chr1.3:32144725-32144744 MS.gene84014:CDS 35.0%
! GTTGAAGGAATCTTTTTCTC+CGG + chr1.3:32144368-32144387 MS.gene84014:CDS 35.0%
! GTTTGTTGCTGATAGGTTAT+TGG + chr1.3:32144652-32144671 MS.gene84014:CDS 35.0%
!!! CAGATTTTTGGGTATTGGAT+TGG - chr1.3:32143933-32143952 None:intergenic 35.0%
!!! TGCTGATTTTTGCTTCAAGT+TGG + chr1.3:32144826-32144845 MS.gene84014:CDS 35.0%
!!! TTTCCCAGATTTTTGGGTAT+TGG - chr1.3:32143938-32143957 None:intergenic 35.0%
!!! TTTTTTTGAGAAGCGTGTTG+GGG + chr1.3:32144757-32144776 MS.gene84014:CDS 35.0%
AAAAAGCTGAAGCCTCATTC+TGG + chr1.3:32143964-32143983 MS.gene84014:CDS 40.0%
GAAAATCGCACAGAAACTTC+CGG - chr1.3:32144390-32144409 None:intergenic 40.0%
GAGCTTATTTCACGTGATGA+AGG + chr1.3:32144455-32144474 MS.gene84014:CDS 40.0%
GTATCGCGAAAAAAGCTGAT+TGG + chr1.3:32144285-32144304 MS.gene84014:CDS 40.0%
GTTGCATTTGCTTGTGTTGA+AGG + chr1.3:32144353-32144372 MS.gene84014:CDS 40.0%
TCAGAATCCGTTTGATTGGA+TGG + chr1.3:32144706-32144725 MS.gene84014:CDS 40.0%
TCGTTTGAGAAGGTTAATCC+CGG - chr1.3:32144437-32144456 None:intergenic 40.0%
! GAAGTTTCTGTGCGATTTTC+TGG + chr1.3:32144390-32144409 MS.gene84014:CDS 40.0%
! GATGGAATTGATTTCGCTTC+AGG + chr1.3:32144724-32144743 MS.gene84014:CDS 40.0%
! GGATGTTTTCGATTGCAATC+TGG - chr1.3:32144175-32144194 None:intergenic 40.0%
! TGGAATTGATTTCGCTTCAG+GGG + chr1.3:32144726-32144745 MS.gene84014:CDS 40.0%
!!! TCAGCTTTTTTCGCGATACA+TGG - chr1.3:32144284-32144303 None:intergenic 40.0%
AAGCTGATTGGGCGATGAAA+TGG + chr1.3:32144297-32144316 MS.gene84014:CDS 45.0%
ACAATTTCTTTCACGCGCTC+TGG - chr1.3:32144536-32144555 None:intergenic 45.0%
CACTGGCAAAACCTAACAGA+CGG + chr1.3:32144023-32144042 MS.gene84014:CDS 45.0%
GATAGGTTATTGGGTGAGCT+TGG + chr1.3:32144662-32144681 MS.gene84014:CDS 45.0%
GGATTGGGAACATGCAGAAT+CGG - chr1.3:32143917-32143936 None:intergenic 45.0%
TGCAATCTGGAAACCGTAGA+AGG - chr1.3:32144162-32144181 None:intergenic 45.0%
! GAAGAGAGTACATCTCAGAG+TGG - chr1.3:32144196-32144215 None:intergenic 45.0%
! GGAAAGATCGACTTCTTCTG+CGG - chr1.3:32143991-32144010 None:intergenic 45.0%
! GTTTTGCCAGTGCTTGAGAT+CGG - chr1.3:32144015-32144034 None:intergenic 45.0%
ATCGTCCTCGAAAATCTCGC+CGG + chr1.3:32144098-32144117 MS.gene84014:CDS 50.0%
ATGCAGAATCGGTCTGGGTT+TGG - chr1.3:32143906-32143925 None:intergenic 50.0%
GGAACATGCAGAATCGGTCT+GGG - chr1.3:32143911-32143930 None:intergenic 50.0%
GGGTGAGCTTGGTTGTGAAA+AGG + chr1.3:32144673-32144692 MS.gene84014:CDS 50.0%
TGCAGAATCGGTCTGGGTTT+GGG - chr1.3:32143905-32143924 None:intergenic 50.0%
TTCATCCCAACCAATGCGCA+AGG - chr1.3:32144605-32144624 None:intergenic 50.0%
TTCTTCTGCGGTCCAGAATG+AGG - chr1.3:32143979-32143998 None:intergenic 50.0%
TTTCCTCCGATCTCAAGCAC+TGG + chr1.3:32144006-32144025 MS.gene84014:CDS 50.0%
! AGGGGTTCTTCTGGAAAAGC+AGG - chr1.3:32143876-32143895 None:intergenic 50.0%
! ATCTACCGGCGAGATTTTCG+AGG - chr1.3:32144106-32144125 None:intergenic 50.0%
!! ATTTTCGAGGACGATGCCGT+CGG - chr1.3:32144093-32144112 None:intergenic 50.0%
!! CGCATTGGTTGGGATGAATG+GGG + chr1.3:32144607-32144626 MS.gene84014:CDS 50.0%
!! GCATTGGTTGGGATGAATGG+GGG + chr1.3:32144608-32144627 MS.gene84014:CDS 50.0%
!! GCGCATTGGTTGGGATGAAT+GGG + chr1.3:32144606-32144625 MS.gene84014:CDS 50.0%
!! TGCGCATTGGTTGGGATGAA+TGG + chr1.3:32144605-32144624 MS.gene84014:CDS 50.0%
CGAGGACGTGAGAGATGAAG+TGG - chr1.3:32144055-32144074 None:intergenic 55.0%
GCAGAATCGGTCTGGGTTTG+GGG - chr1.3:32143904-32143923 None:intergenic 55.0%
GGGAACATGCAGAATCGGTC+TGG - chr1.3:32143912-32143931 None:intergenic 55.0%
TCCGAAGCACGTGCCTTCTA+CGG + chr1.3:32144146-32144165 MS.gene84014:CDS 55.0%
TTGCCAGTGCTTGAGATCGG+AGG - chr1.3:32144012-32144031 None:intergenic 55.0%
! ACCGTAGAAGGCACGTGCTT+CGG - chr1.3:32144150-32144169 None:intergenic 55.0%
! GTCTGGGTTTGGGGCAAGAA+GGG - chr1.3:32143895-32143914 None:intergenic 55.0%
! TCTGGGTTTGGGGCAAGAAG+GGG - chr1.3:32143894-32143913 None:intergenic 55.0%
!! CGCTTCCTTGCGCATTGGTT+GGG + chr1.3:32144597-32144616 MS.gene84014:CDS 55.0%
AGGCACGTGCTTCGGAGACT+TGG - chr1.3:32144142-32144161 None:intergenic 60.0%
AGTGGCGTTCGCCGTCTGTT+AGG - chr1.3:32144037-32144056 None:intergenic 60.0%
GGGGCAAGAAGGGGTTCTTC+TGG - chr1.3:32143885-32143904 None:intergenic 60.0%
! GCGCTTCCTTGCGCATTGGT+TGG + chr1.3:32144596-32144615 MS.gene84014:CDS 60.0%
! GGTCTGGGTTTGGGGCAAGA+AGG - chr1.3:32143896-32143915 None:intergenic 60.0%
CGACGCGCTTCCTTGCGCAT+TGG + chr1.3:32144592-32144611 MS.gene84014:CDS 65.0%
TTCGAGGACGATGCCGTCGG+AGG - chr1.3:32144090-32144109 None:intergenic 65.0%
GAGGACGATGCCGTCGGAGG+CGG - chr1.3:32144087-32144106 None:intergenic 70.0%
GCCTTCTTCGCCGCCTCCGA+CGG + chr1.3:32144074-32144093 MS.gene84014:CDS 70.0%
CGGAGGCGGCGAAGAAGGCG+AGG - chr1.3:32144073-32144092 None:intergenic 75.0%
GCCGTCGGAGGCGGCGAAGA+AGG - chr1.3:32144078-32144097 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 32143870 32144862 32143870 ID=MS.gene84014
chr1.3 mRNA 32143870 32144862 32143870 ID=MS.gene84014.t1;Parent=MS.gene84014
chr1.3 exon 32143870 32144862 32143870 ID=MS.gene84014.t1.exon1;Parent=MS.gene84014.t1
chr1.3 CDS 32143870 32144862 32143870 ID=cds.MS.gene84014.t1;Parent=MS.gene84014.t1
Gene Sequence

>MS.gene84014

ATGCCTGCTTTTCCAGAAGAACCCCTTCTTGCCCCAAACCCAGACCGATTCTGCATGTTCCCAATCCAATACCCAAAAATCTGGGAAATGTACAAAAAAGCTGAAGCCTCATTCTGGACCGCAGAAGAAGTCGATCTTTCCTCCGATCTCAAGCACTGGCAAAACCTAACAGACGGCGAACGCCACTTCATCTCTCACGTCCTCGCCTTCTTCGCCGCCTCCGACGGCATCGTCCTCGAAAATCTCGCCGGTAGATTCATGAAAGAAATCCAAGTCTCCGAAGCACGTGCCTTCTACGGTTTCCAGATTGCAATCGAAAACATCCACTCTGAGATGTACTCTCTTCTCCTTGAAACGTATATAAGCGATTCTGCTGAAAAGAATCGCTTATTTCATGCAATTGAAACAATTCCATGTATCGCGAAAAAAGCTGATTGGGCGATGAAATGGATCGATTCAAGCGATTCTTTTGCAGAAAGAATCGTTGCATTTGCTTGTGTTGAAGGAATCTTTTTCTCCGGAAGTTTCTGTGCGATTTTCTGGTTGAAAAAACGTGGTTTAATGCCGGGATTAACCTTCTCAAACGAGCTTATTTCACGTGATGAAGGATTACACTGTGATTTCGCTTGTTTGTTATACTCACTTCTACGCAAGAAGCTAAGTCCAGAGCGCGTGAAAGAAATTGTTTGTGATGCTGTTGAAATTGAAAGGGAGTTTGTGTGCGACGCGCTTCCTTGCGCATTGGTTGGGATGAATGGGGGTTTGATGAGTACGTATATTGAGTTTGTTGCTGATAGGTTATTGGGTGAGCTTGGTTGTGAAAAGGTTTACAATGTTCAGAATCCGTTTGATTGGATGGAATTGATTTCGCTTCAGGGGAAAACCAATTTTTTTGAGAAGCGTGTTGGGGAGTATCAGAAAGCTTCTGTTATGAATAGCTTGAATGGTAATGGTGCTGCTGATTTTTGCTTCAAGTTGGATGAAGATTTTTAG

Protein sequence

>MS.gene84014.t1

MPAFPEEPLLAPNPDRFCMFPIQYPKIWEMYKKAEASFWTAEEVDLSSDLKHWQNLTDGERHFISHVLAFFAASDGIVLENLAGRFMKEIQVSEARAFYGFQIAIENIHSEMYSLLLETYISDSAEKNRLFHAIETIPCIAKKADWAMKWIDSSDSFAERIVAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSPERVKEIVCDAVEIEREFVCDALPCALVGMNGGLMSTYIEFVADRLLGELGCEKVYNVQNPFDWMELISLQGKTNFFEKRVGEYQKASVMNSLNGNGAADFCFKLDEDF