AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91310.t1 MTR_1g106210 99.029 206 2 0 12 217 56 261 3.16e-148 413
MS.gene91310.t1 MTR_5g005040 39.333 150 63 5 65 212 109 232 6.51e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91310.t1 AT3G19650 37.805 164 89 6 56 217 150 302 8.58e-12 63.5

Find 53 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTCCTTTCATTCCTTTC+TGG 0.052010 1.3:-72977057 None:intergenic
ATCTGATCATCCTCATTCTT+TGG 0.243825 1.3:-72977327 None:intergenic
TGAGGAAAAGGCCAGCATTT+AGG 0.247990 1.3:+72976953 MS.gene91310:CDS
TTTCTGGAGGGTGGCTAGTA+TGG 0.266311 1.3:-72977041 None:intergenic
TGAGAAATCACTGGCTAAAC+TGG 0.294321 1.3:+72976870 MS.gene91310:CDS
GAGGAAAAGGCCAGCATTTA+GGG 0.296936 1.3:+72976954 MS.gene91310:CDS
TTCTCTGTTTGAGTCTTAAT+AGG 0.302168 1.3:-72977261 None:intergenic
CTTCCTAGGTTCTCGCTTGT+TGG 0.303209 1.3:-72977145 None:intergenic
AGAGGAAGAGACAAATTTAA+TGG 0.334230 1.3:+72977222 MS.gene91310:CDS
TTTGACCTGTCTCCAACCTT+TGG 0.360011 1.3:-72976831 None:intergenic
TCCTCTGCAATTCGCTTCTC+AGG 0.377354 1.3:-72977114 None:intergenic
GCTTCTCAGGTCTATCTAGA+TGG 0.381632 1.3:-72977101 None:intergenic
TCAGGTCTATCTAGATGGTT+AGG 0.410188 1.3:-72977096 None:intergenic
GATGAAAATCCATCCTTCCT+AGG 0.435435 1.3:-72977159 None:intergenic
TCCTTTCATTCCTTTCTGGA+GGG 0.447281 1.3:-72977053 None:intergenic
GGATGATCAGATTGCAAAGC+TGG 0.457045 1.3:+72977338 MS.gene91310:CDS
TTCCTTTCATTCCTTTCTGG+AGG 0.457901 1.3:-72977054 None:intergenic
CAACACCTTCTGTGAGGAAA+AGG 0.461132 1.3:+72976941 MS.gene91310:CDS
TTTACTGTCACTGTCGCAGC+TGG 0.462931 1.3:-72977012 None:intergenic
ATCTTCTTCTCCCTAAATGC+TGG 0.485997 1.3:-72976964 None:intergenic
AAAGGTATATGCTCAAAGTA+AGG 0.495225 1.3:+72976801 MS.gene91310:CDS
TTTCATTCCTTTCTGGAGGG+TGG 0.506208 1.3:-72977050 None:intergenic
TTTCATCAAGAGTAAGGTAT+GGG 0.512420 1.3:+72977175 MS.gene91310:CDS
TGGTCGAAGCACTGGTCAAA+GGG 0.514223 1.3:+72976652 MS.gene91310:CDS
GTGCAGGTTGGTCGAAGCAC+TGG 0.521659 1.3:+72976644 MS.gene91310:CDS
AGAAGCGAATTGCAGAGGAC+AGG 0.525004 1.3:+72977118 MS.gene91310:CDS
TAAGGAGAATAACCAAAGGT+TGG 0.542846 1.3:+72976819 MS.gene91310:CDS
AATAACCAAAGGTTGGAGAC+AGG 0.542889 1.3:+72976826 MS.gene91310:CDS
TTGGTCGAAGCACTGGTCAA+AGG 0.551358 1.3:+72976651 MS.gene91310:CDS
GAAGCGAATTGCAGAGGACA+GGG 0.551739 1.3:+72977119 MS.gene91310:CDS
TTAAGACTCAAACAGAGAAG+TGG 0.554278 1.3:+72977265 MS.gene91310:CDS
GCAAAGAGATGAGAAATCAC+TGG 0.563837 1.3:+72976861 MS.gene91310:CDS
AAGCGAGAACCTAGGAAGGA+TGG 0.574352 1.3:+72977150 MS.gene91310:CDS
ACCCTCCAGAAAGGAATGAA+AGG 0.576042 1.3:+72977052 MS.gene91310:CDS
TCCAGAAAGGAATGAAAGGA+AGG 0.580588 1.3:+72977056 MS.gene91310:CDS
ATACTAGCCACCCTCCAGAA+AGG 0.585699 1.3:+72977043 MS.gene91310:CDS
GAAACATGATTTGTATCAAG+AGG 0.586742 1.3:+72977287 MS.gene91310:CDS
AAATCACTGGCTAAACTGGA+TGG 0.588753 1.3:+72976874 MS.gene91310:CDS
AGATTCTATTCCAAAGAATG+AGG 0.590814 1.3:+72977317 MS.gene91310:CDS
ACCTGAGAAGCGAATTGCAG+AGG 0.607322 1.3:+72977113 MS.gene91310:CDS
TGAATGGGGCAATACTGTGC+AGG 0.607456 1.3:+72976628 MS.gene91310:CDS
TTCATCAAGAGTAAGGTATG+GGG 0.620262 1.3:+72977176 MS.gene91310:CDS
CAACAAGCGAGAACCTAGGA+AGG 0.622024 1.3:+72977146 MS.gene91310:CDS
GATCTCAGTGGATTCAAGAG+AGG 0.625358 1.3:+72976984 MS.gene91310:CDS
AGAAAGGAATGAAAGGAAGG+AGG 0.636511 1.3:+72977059 MS.gene91310:CDS
TCATCAAGAGTAAGGTATGG+GGG 0.637056 1.3:+72977177 MS.gene91310:CDS
AATCCAACAAGCGAGAACCT+AGG 0.638185 1.3:+72977142 MS.gene91310:CDS
ACTGGTCAAAGGGAAGCTGA+TGG 0.653810 1.3:+72976662 MS.gene91310:CDS
TGGGGCAATACTGTGCAGGT+TGG 0.672427 1.3:+72976632 MS.gene91310:CDS
AAAGTAAGGAGAATAACCAA+AGG 0.679155 1.3:+72976815 MS.gene91310:CDS
ATGATCCAACACCTTCTGTG+AGG 0.687855 1.3:+72976935 MS.gene91310:CDS
TAGGGAGAAGAAGATCTCAG+TGG 0.726006 1.3:+72976972 MS.gene91310:CDS
GGAGGCAATAGCAATCACAG+AGG 0.738956 1.3:+72977204 MS.gene91310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATATATATTATTTTCTTT+TGG + chr1.3:72976706-72976725 MS.gene91310:intron 10.0%
! TTTATTGTATATGGATGGTT+TGG + chr1.3:72976730-72976749 MS.gene91310:intron 25.0%
!!! TCTTTTGGCTTTATTGTATA+TGG + chr1.3:72976721-72976740 MS.gene91310:intron 25.0%
!!! TGTATGCAAACTATTTTTCT+GGG + chr1.3:72976529-72976548 MS.gene91310:intron 25.0%
AAAGTAAGGAGAATAACCAA+AGG + chr1.3:72976815-72976834 MS.gene91310:CDS 30.0%
AGAGGAAGAGACAAATTTAA+TGG + chr1.3:72977222-72977241 MS.gene91310:CDS 30.0%
AGATTCTATTCCAAAGAATG+AGG + chr1.3:72977317-72977336 MS.gene91310:CDS 30.0%
TTCTCTGTTTGAGTCTTAAT+AGG - chr1.3:72977264-72977283 None:intergenic 30.0%
TTTCATCAAGAGTAAGGTAT+GGG + chr1.3:72977175-72977194 MS.gene91310:CDS 30.0%
! AAAGGTATATGCTCAAAGTA+AGG + chr1.3:72976801-72976820 MS.gene91310:CDS 30.0%
! ATGGATTTTCATCAAGAGTA+AGG + chr1.3:72977169-72977188 MS.gene91310:CDS 30.0%
! GAAACATGATTTGTATCAAG+AGG + chr1.3:72977287-72977306 MS.gene91310:CDS 30.0%
! TTTTAACAGCATCATGAATG+GGG + chr1.3:72976614-72976633 MS.gene91310:intron 30.0%
! TTTTCATCAAGAGTAAGGTA+TGG + chr1.3:72977174-72977193 MS.gene91310:CDS 30.0%
!! GTATGCAAACTATTTTTCTG+GGG + chr1.3:72976530-72976549 MS.gene91310:intron 30.0%
!! GTGTATGCAAACTATTTTTC+TGG + chr1.3:72976528-72976547 MS.gene91310:intron 30.0%
!! GTTTGGAATCGAAGAATTTT+GGG - chr1.3:72976778-72976797 None:intergenic 30.0%
!! GTTTTAACAGCATCATGAAT+GGG + chr1.3:72976613-72976632 MS.gene91310:intron 30.0%
!! TGTTTGGAATCGAAGAATTT+TGG - chr1.3:72976779-72976798 None:intergenic 30.0%
!! TTGGCTTTATTGTATATGGA+TGG + chr1.3:72976725-72976744 MS.gene91310:intron 30.0%
!!! TGTTTTAACAGCATCATGAA+TGG + chr1.3:72976612-72976631 MS.gene91310:intron 30.0%
ATCTGATCATCCTCATTCTT+TGG - chr1.3:72977330-72977349 None:intergenic 35.0%
GATGCTGTTAAAACATGCAT+TGG - chr1.3:72976607-72976626 None:intergenic 35.0%
TAAGGAGAATAACCAAAGGT+TGG + chr1.3:72976819-72976838 MS.gene91310:CDS 35.0%
TCTTCGATTCCAAACAGAAA+AGG + chr1.3:72976783-72976802 MS.gene91310:intron 35.0%
TTAAGACTCAAACAGAGAAG+TGG + chr1.3:72977265-72977284 MS.gene91310:CDS 35.0%
TTCATCAAGAGTAAGGTATG+GGG + chr1.3:72977176-72977195 MS.gene91310:CDS 35.0%
! GAGCATATACCTTTTCTGTT+TGG - chr1.3:72976795-72976814 None:intergenic 35.0%
AATAACCAAAGGTTGGAGAC+AGG + chr1.3:72976826-72976845 MS.gene91310:CDS 40.0%
AGAAAGGAATGAAAGGAAGG+AGG + chr1.3:72977059-72977078 MS.gene91310:CDS 40.0%
ATCTTCTTCTCCCTAAATGC+TGG - chr1.3:72976967-72976986 None:intergenic 40.0%
GATGAAAATCCATCCTTCCT+AGG - chr1.3:72977162-72977181 None:intergenic 40.0%
GCAAAGAGATGAGAAATCAC+TGG + chr1.3:72976861-72976880 MS.gene91310:CDS 40.0%
TCAGGTCTATCTAGATGGTT+AGG - chr1.3:72977099-72977118 None:intergenic 40.0%
TCATCAAGAGTAAGGTATGG+GGG + chr1.3:72977177-72977196 MS.gene91310:CDS 40.0%
TCCAGAAAGGAATGAAAGGA+AGG + chr1.3:72977056-72977075 MS.gene91310:CDS 40.0%
TCCTTCCTTTCATTCCTTTC+TGG - chr1.3:72977060-72977079 None:intergenic 40.0%
TCCTTTCATTCCTTTCTGGA+GGG - chr1.3:72977056-72977075 None:intergenic 40.0%
TTCCTTTCATTCCTTTCTGG+AGG - chr1.3:72977057-72977076 None:intergenic 40.0%
! AAATCACTGGCTAAACTGGA+TGG + chr1.3:72976874-72976893 MS.gene91310:CDS 40.0%
! TGAGAAATCACTGGCTAAAC+TGG + chr1.3:72976870-72976889 MS.gene91310:CDS 40.0%
!! GAAGAGACAGTTTTCGAGAA+AGG + chr1.3:72976908-72976927 MS.gene91310:CDS 40.0%
!!! AAACTATTTTTCTGGGGCGT+GGG + chr1.3:72976536-72976555 MS.gene91310:intron 40.0%
AATCCAACAAGCGAGAACCT+AGG + chr1.3:72977142-72977161 MS.gene91310:CDS 45.0%
ACCCTCCAGAAAGGAATGAA+AGG + chr1.3:72977052-72977071 MS.gene91310:CDS 45.0%
ATGATCCAACACCTTCTGTG+AGG + chr1.3:72976935-72976954 MS.gene91310:CDS 45.0%
CAACACCTTCTGTGAGGAAA+AGG + chr1.3:72976941-72976960 MS.gene91310:CDS 45.0%
GAGGAAAAGGCCAGCATTTA+GGG + chr1.3:72976954-72976973 MS.gene91310:CDS 45.0%
GCTTCTCAGGTCTATCTAGA+TGG - chr1.3:72977104-72977123 None:intergenic 45.0%
GGATGATCAGATTGCAAAGC+TGG + chr1.3:72977338-72977357 MS.gene91310:CDS 45.0%
TAGGGAGAAGAAGATCTCAG+TGG + chr1.3:72976972-72976991 MS.gene91310:CDS 45.0%
TGAGGAAAAGGCCAGCATTT+AGG + chr1.3:72976953-72976972 MS.gene91310:CDS 45.0%
TTTGACCTGTCTCCAACCTT+TGG - chr1.3:72976834-72976853 None:intergenic 45.0%
! GATCTCAGTGGATTCAAGAG+AGG + chr1.3:72976984-72977003 MS.gene91310:CDS 45.0%
! GCATTGGAAAGCATATACCG+AGG - chr1.3:72976591-72976610 None:intergenic 45.0%
! TTTCATTCCTTTCTGGAGGG+TGG - chr1.3:72977053-72977072 None:intergenic 45.0%
!! CTTTTCCTCACAGAAGGTGT+TGG - chr1.3:72976943-72976962 None:intergenic 45.0%
!!! CAAACTATTTTTCTGGGGCG+TGG + chr1.3:72976535-72976554 MS.gene91310:intron 45.0%
!! TATATATCAATATAAAATTA+AGG - chr1.3:72976695-72976714 None:intergenic 5.0%
AAGCGAGAACCTAGGAAGGA+TGG + chr1.3:72977150-72977169 MS.gene91310:CDS 50.0%
ACCTGAGAAGCGAATTGCAG+AGG + chr1.3:72977113-72977132 MS.gene91310:CDS 50.0%
ACTGGTCAAAGGGAAGCTGA+TGG + chr1.3:72976662-72976681 MS.gene91310:CDS 50.0%
AGAAGCGAATTGCAGAGGAC+AGG + chr1.3:72977118-72977137 MS.gene91310:CDS 50.0%
AGAGTAAGGTATGGGGGAAG+TGG + chr1.3:72977183-72977202 MS.gene91310:CDS 50.0%
ATACTAGCCACCCTCCAGAA+AGG + chr1.3:72977043-72977062 MS.gene91310:CDS 50.0%
CAACAAGCGAGAACCTAGGA+AGG + chr1.3:72977146-72977165 MS.gene91310:CDS 50.0%
CTTCCTAGGTTCTCGCTTGT+TGG - chr1.3:72977148-72977167 None:intergenic 50.0%
GAAGCGAATTGCAGAGGACA+GGG + chr1.3:72977119-72977138 MS.gene91310:CDS 50.0%
GGAGGCAATAGCAATCACAG+AGG + chr1.3:72977204-72977223 MS.gene91310:CDS 50.0%
TCCTCTGCAATTCGCTTCTC+AGG - chr1.3:72977117-72977136 None:intergenic 50.0%
TGAATGGGGCAATACTGTGC+AGG + chr1.3:72976628-72976647 MS.gene91310:CDS 50.0%
TTTACTGTCACTGTCGCAGC+TGG - chr1.3:72977015-72977034 None:intergenic 50.0%
! GCATATACCGAGGCACTTGA+AGG - chr1.3:72976581-72976600 None:intergenic 50.0%
! TTTCTGGAGGGTGGCTAGTA+TGG - chr1.3:72977044-72977063 None:intergenic 50.0%
!! TGGTCGAAGCACTGGTCAAA+GGG + chr1.3:72976652-72976671 MS.gene91310:CDS 50.0%
!! TTGGTCGAAGCACTGGTCAA+AGG + chr1.3:72976651-72976670 MS.gene91310:CDS 50.0%
GTAAGGTATGGGGGAAGTGG+AGG + chr1.3:72977186-72977205 MS.gene91310:CDS 55.0%
TGGGGCAATACTGTGCAGGT+TGG + chr1.3:72976632-72976651 MS.gene91310:CDS 55.0%
! ACCGAGGCACTTGAAGGCAT+GGG - chr1.3:72976575-72976594 None:intergenic 55.0%
! TACCGAGGCACTTGAAGGCA+TGG - chr1.3:72976576-72976595 None:intergenic 55.0%
!!! GCTGGCCTTTTCCTCACAGA+AGG - chr1.3:72976949-72976968 None:intergenic 55.0%
CCCCATGCCTTCAAGTGCCT+CGG + chr1.3:72976571-72976590 MS.gene91310:intron 60.0%
! CCGAGGCACTTGAAGGCATG+GGG - chr1.3:72976574-72976593 None:intergenic 60.0%
!! GTGCAGGTTGGTCGAAGCAC+TGG + chr1.3:72976644-72976663 MS.gene91310:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 72976518 72977380 72976518 ID=MS.gene91310
chr1.3 mRNA 72976518 72977380 72976518 ID=MS.gene91310.t1;Parent=MS.gene91310
chr1.3 exon 72976518 72976529 72976518 ID=MS.gene91310.t1.exon1;Parent=MS.gene91310.t1
chr1.3 CDS 72976518 72976529 72976518 ID=cds.MS.gene91310.t1;Parent=MS.gene91310.t1
chr1.3 exon 72976623 72976683 72976623 ID=MS.gene91310.t1.exon2;Parent=MS.gene91310.t1
chr1.3 CDS 72976623 72976683 72976623 ID=cds.MS.gene91310.t1;Parent=MS.gene91310.t1
chr1.3 exon 72976800 72977380 72976800 ID=MS.gene91310.t1.exon3;Parent=MS.gene91310.t1
chr1.3 CDS 72976800 72977380 72976800 ID=cds.MS.gene91310.t1;Parent=MS.gene91310.t1
Gene Sequence

>MS.gene91310

ATGTTAATCAGTGTATGCAAACTATTTTTCTGGGGCGTGGGATTCTTATTCAGCCCCATGCCTTCAAGTGCCTCGGTATATGCTTTCCAATGCATGTTTTAACAGCATCATGAATGGGGCAATACTGTGCAGGTTGGTCGAAGCACTGGTCAAAGGGAAGCTGATGGTGAGTTTCCTTAATTTTATATTGATATATATTATTTTCTTTTGGCTTTATTGTATATGGATGGTTTGGAGATTTACTCGATATACTGAAGCCCAAAATTCTTCGATTCCAAACAGAAAAGGTATATGCTCAAAGTAAGGAGAATAACCAAAGGTTGGAGACAGGTCAAAGTAGAGAGCAAAGAGATGAGAAATCACTGGCTAAACTGGATGGTAATTTTCAGAGAAGAGACAGTTTTCGAGAAAGGAAAGATGATCCAACACCTTCTGTGAGGAAAAGGCCAGCATTTAGGGAGAAGAAGATCTCAGTGGATTCAAGAGAGGCTAATCCAGCTGCGACAGTGACAGTAAAGTCAAGCCATACTAGCCACCCTCCAGAAAGGAATGAAAGGAAGGAGGAAAGAAGCAGCAATCCTAACCATCTAGATAGACCTGAGAAGCGAATTGCAGAGGACAGGGAATCCAACAAGCGAGAACCTAGGAAGGATGGATTTTCATCAAGAGTAAGGTATGGGGGAAGTGGAGGCAATAGCAATCACAGAGGAAGAGACAAATTTAATGGAAGACAAGATAATCATCCTATTAAGACTCAAACAGAGAAGTGGAAACATGATTTGTATCAAGAGGTTAACAAAGATTCTATTCCAAAGAATGAGGATGATCAGATTGCAAAGCTGGAAGCACTCTTAGCTTCATAA

Protein sequence

>MS.gene91310.t1

MLISHHEWGNTVQVGRSTGQREADEKVYAQSKENNQRLETGQSREQRDEKSLAKLDGNFQRRDSFRERKDDPTPSVRKRPAFREKKISVDSREANPAATVTVKSSHTSHPPERNERKEERSSNPNHLDRPEKRIAEDRESNKREPRKDGFSSRVRYGGSGGNSNHRGRDKFNGRQDNHPIKTQTEKWKHDLYQEVNKDSIPKNEDDQIAKLEALLAS