AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93942


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93942.t1 AT1G72030 52.747 182 80 3 14 190 60 240 3.66e-53 171

Find 34 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGACATTATTTCCAATTTA+TGG 0.063814 4.3:-66374798 MS.gene93942:CDS
CAAGAGTACCTTTACATTTC+AGG 0.191164 4.3:-66375134 MS.gene93942:CDS
TAGCTGTCTCCAATGCTTTC+AGG 0.230533 4.3:-66375109 MS.gene93942:intron
AAACTCATTACCCCATAAAT+TGG 0.252387 4.3:+66374786 None:intergenic
TAGAGCTTATGAGGAAGATT+TGG 0.261696 4.3:-66374755 MS.gene93942:CDS
TATATATAGGTATGCTTGTT+TGG 0.294474 4.3:-66375258 MS.gene93942:intron
TGACATTATTTCCAATTTAT+GGG 0.303190 4.3:-66374797 MS.gene93942:CDS
AAATAGGCTGAAGTGCTTTC+AGG 0.334616 4.3:-66375491 MS.gene93942:intron
AGAGCTTATGAGGAAGATTT+GGG 0.386323 4.3:-66374754 MS.gene93942:CDS
AATCTTCCTCATAAGCTCTA+AGG 0.389371 4.3:+66374758 None:intergenic
TAACAACAAATGTACCTATC+AGG 0.398497 4.3:+66375440 None:intergenic
TGAAAATATGAAGCAAAAGT+TGG 0.405502 4.3:-66376162 MS.gene93942:CDS
TCTTGTCCTTAGAGCTTATG+AGG 0.416016 4.3:-66374764 MS.gene93942:CDS
GTGAATATGGATGGAGGATA+AGG 0.442867 4.3:-66376127 MS.gene93942:CDS
ATAACACCTACAAGTTCCTT+TGG 0.452679 4.3:+66375212 None:intergenic
TTAAATACTCACCTCCTGAT+AGG 0.463612 4.3:-66375454 MS.gene93942:intron
GATTCACCAAAGGAACTTGT+AGG 0.478737 4.3:-66375218 MS.gene93942:CDS
AAGTCATTGAAAAGAGCTAC+AGG 0.503117 4.3:+66376038 None:intergenic
CGAACATTGTACACAAATGC+TGG 0.511040 4.3:-66374727 MS.gene93942:CDS
AGGTACTCTTGTGCATCAGC+AGG 0.514017 4.3:+66375146 None:intergenic
ACAATCAAAGAGAGAGCTCA+AGG 0.518793 4.3:-66374289 MS.gene93942:CDS
GTGATGTTGGAGATTGAGAA+AGG 0.519667 4.3:-66376188 MS.gene93942:CDS
CAGCTATTCCTGAAATGTAA+AGG 0.520945 4.3:+66375126 None:intergenic
GAAGATGTTCAAGCACATCT+TGG 0.525750 4.3:+66375168 None:intergenic
ATATAGGTATGCTTGTTTGG+TGG 0.525798 4.3:-66375255 MS.gene93942:intron
CCAACCCAATTACTTGTCCA+TGG 0.539516 4.3:+66374679 None:intergenic
GACATTATTTCCAATTTATG+GGG 0.560914 4.3:-66374796 MS.gene93942:CDS
GTGGATCTTTAGACACAAGT+TGG 0.596499 4.3:+66374701 None:intergenic
TGGTGTGTGAATATGGATGG+AGG 0.600815 4.3:-66376133 MS.gene93942:CDS
CAATCAAAGAGAGAGCTCAA+GGG 0.619765 4.3:-66374288 MS.gene93942:CDS
ACCCAATTACTTGTCCATGG+TGG 0.661718 4.3:+66374682 None:intergenic
CAACAAATGTACCTATCAGG+AGG 0.675327 4.3:+66375443 None:intergenic
GAATGATCAAGATTCACCAA+AGG 0.679618 4.3:-66375228 MS.gene93942:CDS
TTGTGTCTAAAGATCCACCA+TGG 0.708310 4.3:-66374696 MS.gene93942:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAAAATAATAATTATTTT+AGG + chr4.3:66375090-66375109 None:intergenic 0.0%
!! AATTAATATTCTACTTAATT+AGG + chr4.3:66375844-66375863 None:intergenic 10.0%
!! TATATTATGCTATTATATAT+AGG - chr4.3:66375183-66375202 MS.gene93942:CDS 10.0%
!!! ATATTCAAGTATTTTTAAAT+AGG - chr4.3:66375403-66375422 MS.gene93942:intron 10.0%
!!! GTCAAAATTTATTATTTTAA+AGG + chr4.3:66374877-66374896 None:intergenic 10.0%
!! ATCATTTATCAATATGAAAT+TGG - chr4.3:66375061-66375080 MS.gene93942:intron 15.0%
!! AAACATTAAACAACAACTAA+AGG + chr4.3:66374927-66374946 None:intergenic 20.0%
!! TAAAGACAGAATATTAGAAA+TGG - chr4.3:66375882-66375901 MS.gene93942:intron 20.0%
!! TGACATTATTTCCAATTTAT+GGG - chr4.3:66375657-66375676 MS.gene93942:intron 20.0%
!! TGTATTATATATGTGAAAGT+CGG + chr4.3:66374727-66374746 None:intergenic 20.0%
!!! CTTGTTTTTTCAATTCTTTA+AGG - chr4.3:66374436-66374455 MS.gene93942:intron 20.0%
! AACTAAAGGCAAATAAAATG+TGG + chr4.3:66374913-66374932 None:intergenic 25.0%
! ATGTTAATGAGACTAATTGA+TGG - chr4.3:66375593-66375612 MS.gene93942:intron 25.0%
! CAATTTATTATCTCATGCTA+AGG + chr4.3:66375135-66375154 None:intergenic 25.0%
! GACATTATTTCCAATTTATG+GGG - chr4.3:66375658-66375677 MS.gene93942:intron 25.0%
! GTGACATTATTTCCAATTTA+TGG - chr4.3:66375656-66375675 MS.gene93942:intron 25.0%
! TATATATAGGTATGCTTGTT+TGG - chr4.3:66375196-66375215 MS.gene93942:CDS 25.0%
! TATTCATTCTTGACACAAAT+AGG - chr4.3:66374947-66374966 MS.gene93942:intron 25.0%
! TGAAAATATGAAGCAAAAGT+TGG - chr4.3:66374292-66374311 MS.gene93942:CDS 25.0%
! TTCTCTACTTATTGTCTTAA+AGG + chr4.3:66374689-66374708 None:intergenic 25.0%
! TTGAACATATCTCAGTTAAT+AGG + chr4.3:66374754-66374773 None:intergenic 25.0%
! TTTGATTGATCTACTGTATA+AGG - chr4.3:66376103-66376122 MS.gene93942:CDS 25.0%
!! AATAGTAACAAGATTTTGAG+TGG - chr4.3:66374549-66374568 MS.gene93942:intron 25.0%
!!! AGTTTTCTTTTTTTGTGTTC+CGG + chr4.3:66375459-66375478 None:intergenic 25.0%
AAACTCATTACCCCATAAAT+TGG + chr4.3:66375671-66375690 None:intergenic 30.0%
AAAGGCAAATAAAATGTGGT+AGG + chr4.3:66374909-66374928 None:intergenic 30.0%
AAGACAATAAGTAGAGAATC+TGG - chr4.3:66374691-66374710 MS.gene93942:intron 30.0%
ATTCTAACCACTACACAATT+TGG + chr4.3:66374662-66374681 None:intergenic 30.0%
ATTTCACCATAAAGAGAGTT+TGG + chr4.3:66374600-66374619 None:intergenic 30.0%
GAAATTGCTTCTCAAATTGT+AGG - chr4.3:66374615-66374634 MS.gene93942:intron 30.0%
GAATGCAGATGAGATAAAAA+TGG - chr4.3:66374361-66374380 MS.gene93942:intron 30.0%
TAACAACAAATGTACCTATC+AGG + chr4.3:66375017-66375036 None:intergenic 30.0%
TGATGACACTGAAATAGTAT+TGG + chr4.3:66375988-66376007 None:intergenic 30.0%
TTCTAACCACTACACAATTT+GGG + chr4.3:66374661-66374680 None:intergenic 30.0%
TTTGATCCAAACTCTCTTTA+TGG - chr4.3:66374591-66374610 MS.gene93942:intron 30.0%
!! GTTTTCTTTTTTTGTGTTCC+GGG + chr4.3:66375458-66375477 None:intergenic 30.0%
!! TTTTAATCACCTGAAAGCAT+TGG + chr4.3:66375357-66375376 None:intergenic 30.0%
!!! GAAGCAAAAGTTGGATTTTT+TGG - chr4.3:66374301-66374320 MS.gene93942:CDS 30.0%
!!! GATTTTTTGGTGTGTGAATA+TGG - chr4.3:66374314-66374333 MS.gene93942:CDS 30.0%
AAAATGTGGTAGGTGCTTAA+TGG + chr4.3:66374899-66374918 None:intergenic 35.0%
AAATATTTGTAACCGAGTCC+CGG - chr4.3:66375437-66375456 MS.gene93942:intron 35.0%
AAGTCATTGAAAAGAGCTAC+AGG + chr4.3:66374419-66374438 None:intergenic 35.0%
AATCTTCCTCATAAGCTCTA+AGG + chr4.3:66375699-66375718 None:intergenic 35.0%
AGAGCTTATGAGGAAGATTT+GGG - chr4.3:66375700-66375719 MS.gene93942:intron 35.0%
ATAACACCTACAAGTTCCTT+TGG + chr4.3:66375245-66375264 None:intergenic 35.0%
ATATAGGTATGCTTGTTTGG+TGG - chr4.3:66375199-66375218 MS.gene93942:CDS 35.0%
ATCAAAGCAGATGTTGAACT+AGG + chr4.3:66376089-66376108 None:intergenic 35.0%
CAGCTATTCCTGAAATGTAA+AGG + chr4.3:66375331-66375350 None:intergenic 35.0%
GAATGATCAAGATTCACCAA+AGG - chr4.3:66375226-66375245 MS.gene93942:CDS 35.0%
TAGAGCTTATGAGGAAGATT+TGG - chr4.3:66375699-66375718 MS.gene93942:intron 35.0%
TATTTCAGTGTCATCAATCG+TGG - chr4.3:66375992-66376011 MS.gene93942:intron 35.0%
TTAAATACTCACCTCCTGAT+AGG - chr4.3:66375000-66375019 MS.gene93942:intron 35.0%
TTGCTTCTCAAATTGTAGGA+TGG - chr4.3:66374619-66374638 MS.gene93942:intron 35.0%
! TAAGTAGAGAATCTGGAGTT+CGG - chr4.3:66374698-66374717 MS.gene93942:CDS 35.0%
! TCTTTTCCCAAATTGTGTAG+TGG - chr4.3:66374652-66374671 MS.gene93942:intron 35.0%
!! CAAGAGTACCTTTACATTTC+AGG - chr4.3:66375320-66375339 MS.gene93942:intron 35.0%
!! TTTTGGTGTGTGAATATGGA+TGG - chr4.3:66374318-66374337 MS.gene93942:CDS 35.0%
ACAATCAAAGAGAGAGCTCA+AGG - chr4.3:66376165-66376184 MS.gene93942:CDS 40.0%
AGAAGGAAAAGACGATTGCA+AGG + chr4.3:66376060-66376079 None:intergenic 40.0%
ATCCACCATGGACAAGTAAT+TGG - chr4.3:66375770-66375789 MS.gene93942:intron 40.0%
CAACAAATGTACCTATCAGG+AGG + chr4.3:66375014-66375033 None:intergenic 40.0%
CAATCAAAGAGAGAGCTCAA+GGG - chr4.3:66376166-66376185 MS.gene93942:CDS 40.0%
CGAACATTGTACACAAATGC+TGG - chr4.3:66375727-66375746 MS.gene93942:intron 40.0%
GAAGATGTTCAAGCACATCT+TGG + chr4.3:66375289-66375308 None:intergenic 40.0%
GATTCACCAAAGGAACTTGT+AGG - chr4.3:66375236-66375255 MS.gene93942:CDS 40.0%
GCTAAACCACTAGTTGCTAA+TGG + chr4.3:66375529-66375548 None:intergenic 40.0%
GTGAATATGGATGGAGGATA+AGG - chr4.3:66374327-66374346 MS.gene93942:CDS 40.0%
GTGGATCTTTAGACACAAGT+TGG + chr4.3:66375756-66375775 None:intergenic 40.0%
TCCACCATGGACAAGTAATT+GGG - chr4.3:66375771-66375790 MS.gene93942:intron 40.0%
TCTTGTCCTTAGAGCTTATG+AGG - chr4.3:66375690-66375709 MS.gene93942:intron 40.0%
TTGTGTCTAAAGATCCACCA+TGG - chr4.3:66375758-66375777 MS.gene93942:intron 40.0%
! AAATAGGCTGAAGTGCTTTC+AGG - chr4.3:66374963-66374982 MS.gene93942:intron 40.0%
! ATGAGACTAATTGATGGCAC+AGG - chr4.3:66375599-66375618 MS.gene93942:intron 40.0%
! GTTGAACTAGGTTTTGCAGA+AGG + chr4.3:66376077-66376096 None:intergenic 40.0%
!!! TTTTTTTGTGTTCCGGGACT+CGG + chr4.3:66375452-66375471 None:intergenic 40.0%
ACCCAATTACTTGTCCATGG+TGG + chr4.3:66375775-66375794 None:intergenic 45.0%
CCAACCCAATTACTTGTCCA+TGG + chr4.3:66375778-66375797 None:intergenic 45.0%
CCATGGACAAGTAATTGGGT+TGG - chr4.3:66375775-66375794 MS.gene93942:intron 45.0%
TAGCTGTCTCCAATGCTTTC+AGG - chr4.3:66375345-66375364 MS.gene93942:intron 45.0%
TCGCTACCATTAGCAACTAG+TGG - chr4.3:66375520-66375539 MS.gene93942:intron 45.0%
TGGTGTGTGAATATGGATGG+AGG - chr4.3:66374321-66374340 MS.gene93942:CDS 45.0%
! AGACTAATTGATGGCACAGG+AGG - chr4.3:66375602-66375621 MS.gene93942:intron 45.0%
AGGTACTCTTGTGCATCAGC+AGG + chr4.3:66375311-66375330 None:intergenic 50.0%
! CTAATTGATGGCACAGGAGG+AGG - chr4.3:66375605-66375624 MS.gene93942:intron 50.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 66374269 66376207 66374269 ID=MS.gene93942
chr4.3 mRNA 66374269 66376207 66374269 ID=MS.gene93942.t1;Parent=MS.gene93942
chr4.3 exon 66376019 66376207 66376019 ID=MS.gene93942.t1.exon1;Parent=MS.gene93942.t1
chr4.3 CDS 66376019 66376207 66376019 ID=cds.MS.gene93942.t1;Parent=MS.gene93942.t1
chr4.3 exon 66375455 66375507 66375455 ID=MS.gene93942.t1.exon2;Parent=MS.gene93942.t1
chr4.3 CDS 66375455 66375507 66375455 ID=cds.MS.gene93942.t1;Parent=MS.gene93942.t1
chr4.3 exon 66375110 66375271 66375110 ID=MS.gene93942.t1.exon3;Parent=MS.gene93942.t1
chr4.3 CDS 66375110 66375271 66375110 ID=cds.MS.gene93942.t1;Parent=MS.gene93942.t1
chr4.3 exon 66374692 66374855 66374692 ID=MS.gene93942.t1.exon4;Parent=MS.gene93942.t1
chr4.3 CDS 66374692 66374855 66374692 ID=cds.MS.gene93942.t1;Parent=MS.gene93942.t1
chr4.3 exon 66374269 66374351 66374269 ID=MS.gene93942.t1.exon5;Parent=MS.gene93942.t1
chr4.3 CDS 66374269 66374351 66374269 ID=cds.MS.gene93942.t1;Parent=MS.gene93942.t1
Gene Sequence

>MS.gene93942

ATGTTGGAGATTGAGAAAGGTAGTGAAAATATGAAGCAAAAGTTGGATTTTTTGGTGTGTGAATATGGATGGAGGATAAGGAGATTGATTGAGAATGCAGATGAGATAAAAATGGCTGCTCAAGTTCAAGCTGAAGCTTTTCATGATCCTGTAGCTCTTTTCAATGACTTGTTTTTTCAATTCTTTAAGGTTTTGTTTCTCTCTTTTTATTAGTGTTTATATTTCATCTTCATTTTTATGTGCTTTTCATATGCAATTTTGTTTCTTAAGAAATTGTTTAAATAGTAACAAGATTTTGAGTGGAACTTGAGTGTAAAAACTGTTTGATCCAAACTCTCTTTATGGTGAAATTGCTTCTCAAATTGTAGGATGGTAAATTTAATTCTTTTCCCAAATTGTGTAGTGGTTAGAATTTCTCCTTTAAGACAATAAGTAGAGAATCTGGAGTTCGGACTCCGACTTTCACATATATAATACAATGTCCTATTAACTGAGATATGTTCAACGAGACAAATTTAGTCTCTTTAGATATAAGAAATTTTAAAATAATTTTTAAAAATTGAAAATGATTAATCTAATAATACTTTTAGATTAGTCAAATTAGTCCTTTAAAATAATAAATTTTGACCATTAAGCACCTACCACATTTTATTTGCCTTTAGTTGTTGTTTAATGTTTTATTCATTCTTGACACAAATAGGCTGAAGTGCTTTCAGGACTGCTTTACAAGCTTAAATACTCACCTCCTGATAGGTACATTTGTTGTTATTTCTAAACAAGTTTATGTAGATTATCATTTATCAATATGAAATTGGTGACCTAAAATAATTATTATTTTTAATTAAAAAAAAACTCATGATAAACCTTAGCATGAGATAATAAATTGATCTTGTGTAAAACATTAGCAACTAATGTATATTATGCTATTATATATAGGTATGCTTGTTTGGTGGCTGAGAATGATCAAGATTCACCAAAGGAACTTGTAGGTGTTATAGATGTTACTGTAATGAGAGACCAAGATGTGCTTGAACATCTTCCTGCTGATGCACAAGAGTACCTTTACATTTCAGGAATAGCTGTCTCCAATGCTTTCAGGTGATTAAAAGATTTAATTACTATTACATCTCTGAAATATTCAAGTATTTTTAAATAGGTCATTGAAATAAAATATTTGTAACCGAGTCCCGGAACACAAAAAAAGAAAACTGTCATATAGTGACTGATTTAGATATCAAAATATCGAATCGATCGCTACCATTAGCAACTAGTGGTTTAGCGACATTTTACACATTGATATTTTTCATGTGATGTGTGAAACTATATGTTAATGAGACTAATTGATGGCACAGGAGGAGGAAAATTGCAACTGTTATGTTGAAAGCATGTGACATTATTTCCAATTTATGGGGTAATGAGTTTCTTGTCCTTAGAGCTTATGAGGAAGATTTGGGTGCTCGAACATTGTACACAAATGCTGGCTACCAACTTGTGTCTAAAGATCCACCATGGACAAGTAATTGGGTTGGAAGAAAATGTCGTGTTCTTATGATCAAAAGAATTAGTTTATTGCCTAATTAAGTAGAATATTAATTCAAAAATTAAGATATATATAAAGACAGAATATTAGAAATGGCAAATGTATTCTATGTATATAGCATATGTCAGCGTATTTAGTTCATTATTTTTCATTGATTACGCACAAAACAAGTTTATCCAATACTATTTCAGTGTCATCAATCGTGGATAGCGAAAAAAAGCATGTTGTTTAAATCGATGAATATGATACCTTGCAATCGTCTTTTCCTTCTGCAAAACCTAGTTCAACATCTGCTTTGATTGATCTACTGTATAAGGTGACACTAGAGAAGTGCAATCTGAGATCGATAGAGAAGAACAATCAAAGAGAGAGCTCAAGGGAGTGAGAGAGAACCGTTGA

Protein sequence

>MS.gene93942.t1

MLEIEKGSENMKQKLDFLVCEYGWRIRRLIENADEIKMAAQVQAEAFHDPVALFNDLFFQFFKAEVLSGLLYKLKYSPPDRYACLVAENDQDSPKELVGVIDVTVMRDQDVLEHLPADAQEYLYISGIAVSNAFRRRKIATVMLKACDIISNLWGNEFLVLRAYEEDLGARTLYTNAGYQLVSKDPPWTSDTREVQSEIDREEQSKRELKGVRENR