Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048137.01.T01 | XP_003600586.1 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 2.46E-42 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048137.01 | MsG0080048197.01 | 0.827967 | 1.167301e-54 | 1.144495e-51 |
| MsG0080048137.01 | MsG0180001043.01 | 0.821558 | 3.788047e-53 | 3.092178e-50 |
| MsG0080048137.01 | MsG0180001599.01 | 0.821489 | 3.929436e-53 | 3.201348e-50 |
| MsG0080048137.01 | MsG0180002283.01 | 0.809249 | 2.077330e-50 | 1.213569e-47 |
| MsG0080048137.01 | MsG0180002489.01 | 0.864729 | 9.856808e-65 | 3.126790e-61 |
| MsG0080048137.01 | MsG0180002491.01 | 0.854745 | 1.002994e-61 | 2.262851e-58 |
| MsG0080048137.01 | MsG0180003088.01 | 0.842086 | 3.205690e-58 | 4.807022e-55 |
| MsG0080048137.01 | MsG0180003793.01 | 0.808535 | 2.953101e-50 | 1.692797e-47 |
| MsG0080048137.01 | MsG0180006013.01 | 0.801497 | 8.757035e-49 | 4.188332e-46 |
| MsG0080048137.01 | MsG0180006016.01 | 0.802533 | 5.363025e-49 | 2.633389e-46 |
| MsG0080048137.01 | MsG0280006411.01 | 0.843652 | 1.228477e-58 | 1.935212e-55 |
| MsG0080048137.01 | MsG0280006422.01 | 0.810358 | 1.199446e-50 | 7.214759e-48 |
| MsG0080048137.01 | MsG0280006595.01 | 0.816372 | 5.721718e-52 | 4.047012e-49 |
| MsG0080048137.01 | MsG0280006596.01 | 0.827858 | 1.240270e-54 | 1.212160e-51 |
| MsG0080048137.01 | MsG0280007161.01 | 0.813780 | 2.152390e-51 | 1.418659e-48 |
| MsG0080048137.01 | MsG0280007439.01 | 0.812010 | 5.257856e-51 | 3.304470e-48 |
| MsG0080048137.01 | MsG0280007715.01 | 0.804252 | 2.361683e-49 | 1.211829e-46 |
| MsG0080048137.01 | MsG0280007847.01 | 0.823687 | 1.211320e-53 | 1.050496e-50 |
| MsG0080048137.01 | MsG0280010416.01 | 0.802903 | 4.497693e-49 | 2.229504e-46 |
| MsG0080048137.01 | MsG0380014330.01 | 0.827668 | 1.377745e-54 | 1.338943e-51 |
| MsG0080048137.01 | MsG0380014561.01 | 0.806888 | 6.609006e-50 | 3.628885e-47 |
| MsG0080048137.01 | MsG0380014885.01 | 0.801219 | 9.984898e-49 | 4.742112e-46 |
| MsG0080048137.01 | MsG0380015027.01 | 0.835594 | 1.532501e-56 | 1.882685e-53 |
| MsG0080048137.01 | MsG0380015157.01 | 0.816713 | 4.798181e-52 | 3.425593e-49 |
| MsG0080048137.01 | MsG0380015922.01 | 0.812573 | 3.960378e-51 | 2.527068e-48 |
| MsG0080048137.01 | MsG0380016559.01 | 0.808610 | 2.845393e-50 | 1.634184e-47 |
| MsG0080048137.01 | MsG0380016560.01 | 0.817323 | 3.499460e-52 | 2.540460e-49 |
| MsG0080048137.01 | MsG0380016604.01 | 0.809470 | 1.862464e-50 | 1.094425e-47 |
| MsG0080048137.01 | MsG0380017226.01 | 0.810011 | 1.424839e-50 | 8.493696e-48 |
| MsG0080048137.01 | MsG0380017540.01 | 0.800942 | 1.137278e-48 | 5.363868e-46 |
| MsG0080048137.01 | MsG0380018001.01 | 0.802090 | 6.617480e-49 | 3.212935e-46 |
| MsG0080048137.01 | MsG0480018906.01 | 0.813623 | 2.331245e-51 | 1.530064e-48 |
| MsG0080048137.01 | MsG0480020372.01 | 0.810755 | 9.843062e-51 | 5.983984e-48 |
| MsG0080048137.01 | MsG0480020416.01 | 0.810858 | 9.352341e-51 | 5.701272e-48 |
| MsG0080048137.01 | MsG0480020791.01 | 0.825170 | 5.422912e-54 | 4.905708e-51 |
| MsG0080048137.01 | MsG0480021041.01 | 0.816578 | 5.145435e-52 | 3.659934e-49 |
| MsG0080048137.01 | MsG0480021448.01 | 0.833273 | 5.862107e-56 | 6.717654e-53 |
| MsG0080048137.01 | MsG0480021453.01 | 0.812531 | 4.045401e-51 | 2.578559e-48 |
| MsG0080048137.01 | MsG0480023088.01 | 0.805838 | 1.100377e-49 | 5.881833e-47 |
| MsG0080048137.01 | MsG0580024191.01 | 0.839302 | 1.719141e-57 | 2.364121e-54 |
| MsG0080048137.01 | MsG0580025134.01 | 0.848608 | 5.498191e-60 | 1.014519e-56 |
| MsG0080048137.01 | MsG0580025756.01 | 0.820383 | 7.062658e-53 | 5.580155e-50 |
| MsG0080048137.01 | MsG0580026047.01 | 0.801093 | 1.059197e-48 | 5.014937e-46 |
| MsG0080048137.01 | MsG0580026301.01 | 0.808561 | 2.914953e-50 | 1.672064e-47 |
| MsG0080048137.01 | MsG0580026777.01 | 0.802908 | 4.487029e-49 | 2.224489e-46 |
| MsG0080048137.01 | MsG0580027034.01 | 0.812798 | 3.536256e-51 | 2.269626e-48 |
| MsG0080048137.01 | MsG0580028289.01 | 0.806683 | 7.302624e-50 | 3.988428e-47 |
| MsG0080048137.01 | MsG0580029661.01 | 0.812529 | 4.049250e-51 | 2.580879e-48 |
| MsG0080048137.01 | MsG0580030095.01 | -0.801312 | 9.555820e-49 | 4.549073e-46 |
| MsG0080048137.01 | MsG0680030415.01 | 0.803354 | 3.628393e-49 | 1.819333e-46 |
| MsG0080048137.01 | MsG0680031712.01 | 0.817421 | 3.326802e-52 | 2.421720e-49 |
| MsG0080048137.01 | MsG0680031964.01 | 0.806581 | 7.674027e-50 | 4.180414e-47 |
| MsG0080048137.01 | MsG0680032430.01 | 0.802966 | 4.366236e-49 | 2.167692e-46 |
| MsG0080048137.01 | MsG0680034061.01 | 0.804827 | 1.791885e-49 | 9.332856e-47 |
| MsG0080048137.01 | MsG0780037503.01 | 0.824148 | 9.444419e-54 | 8.296689e-51 |
| MsG0080048137.01 | MsG0780038911.01 | 0.836229 | 1.057492e-56 | 1.324584e-53 |
| MsG0080048137.01 | MsG0780039340.01 | 0.813130 | 2.991136e-51 | 1.936895e-48 |
| MsG0080048137.01 | MsG0780039943.01 | 0.803149 | 4.000212e-49 | 1.995358e-46 |
| MsG0080048137.01 | MsG0780041223.01 | 0.827742 | 1.322262e-54 | 1.287822e-51 |
| MsG0080048137.01 | MsG0780041295.01 | 0.817111 | 3.907268e-52 | 2.820015e-49 |
| MsG0080048137.01 | MsG0780041490.01 | 0.837153 | 6.148682e-57 | 7.917183e-54 |
| MsG0080048137.01 | MsG0880041969.01 | 0.800520 | 1.386629e-48 | 6.469994e-46 |
| MsG0080048137.01 | MsG0880043082.01 | 0.826322 | 2.891610e-54 | 2.702801e-51 |
| MsG0080048137.01 | MsG0880043465.01 | 0.821276 | 4.399685e-53 | 3.563143e-50 |
| MsG0080048137.01 | MsG0880045951.01 | 0.851465 | 8.717465e-61 | 1.765921e-57 |
| MsG0080048137.01 | MsG0880046058.01 | 0.839579 | 1.456659e-57 | 2.020219e-54 |
| MsG0080048137.01 | MsG0880046377.01 | 0.810977 | 8.815194e-51 | 5.391235e-48 |
| MsG0080048137.01 | MsG0880046733.01 | 0.804480 | 2.117020e-49 | 1.092698e-46 |
| MsG0080048137.01 | MsG0880047116.01 | 0.832675 | 8.256751e-56 | 9.294230e-53 |
| MsG0080048137.01 | MsG0880047607.01 | 0.805593 | 1.238744e-49 | 6.579690e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048137.01.T01 | MTR_3g063970 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 2.98e-46 | 142 |
| MsG0080048137.01.T01 | MTR_3g063970 | 97.917 | 48 | 1 | 0 | 1 | 48 | 1 | 48 | 6.63e-16 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048137.01.T01 | AT3G14430 | 65.079 | 63 | 21 | 1 | 9 | 71 | 15 | 76 | 1.08e-24 | 87.8 |
Find 17 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACATCAATTCCTTTAAA+AGG | 0.150864 | contig218end:-7085 | MsG0080048137.01.T01:intergenic |
| ATTTCTTACGAAGGCGAAAA+TGG | 0.322719 | contig218end:-6070 | MsG0080048137.01.T01:intergenic |
| ACAAATACTTGATTGTATTC+AGG | 0.336136 | contig218end:-7030 | MsG0080048137.01.T01:intergenic |
| CTGGTGGTGGACCAGAGCTT+TGG | 0.389186 | contig218end:+7061 | MsG0080048137.01.T01:CDS |
| CTTTAAAAGGGCCAAAGCTC+TGG | 0.402112 | contig218end:-7072 | MsG0080048137.01.T01:intergenic |
| AAACATCAATTCCTTTAAAA+GGG | 0.443719 | contig218end:-7084 | MsG0080048137.01.T01:intergenic |
| CAAAGCTCTGGTCCACCACC+AGG | 0.488950 | contig218end:-7060 | MsG0080048137.01.T01:intergenic |
| CAAGTATTTGTGAAGAAGCC+TGG | 0.508253 | contig218end:+7042 | MsG0080048137.01.T01:CDS |
| CAGAGAGAAGAAGACTAGAC+TGG | 0.543222 | contig218end:+5956 | MsG0080048137.01.T01:five_prime_UTR |
| TGAAGAAGAAAAGAAAGAAG+AGG | 0.565354 | contig218end:-6107 | MsG0080048137.01.T01:intergenic |
| GGAACGAGTCTTTATTGACT+CGG | 0.584655 | contig218end:+6037 | MsG0080048137.01.T01:CDS |
| AGAGAACAGCGATGGCTAGA+CGG | 0.584999 | contig218end:+6001 | MsG0080048137.01.T01:exon |
| CTAGACGGCGAGTCGAGACT+CGG | 0.585126 | contig218end:+6016 | MsG0080048137.01.T01:CDS |
| TTTGTGAAGAAGCCTGGTGG+TGG | 0.604245 | contig218end:+7048 | MsG0080048137.01.T01:CDS |
| GAGGTGAGAGAGAACAGCGA+TGG | 0.620676 | contig218end:+5993 | MsG0080048137.01.T01:exon |
| AAACTCAGCATTTCTTACGA+AGG | 0.638252 | contig218end:-6079 | MsG0080048137.01.T01:intergenic |
| GTATTTGTGAAGAAGCCTGG+TGG | 0.787083 | contig218end:+7045 | MsG0080048137.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAAAGAGAG+AGG | - | contig218end:6170-6189 | MsG0080048137.01.T01:intergenic | 15.0% |
| !! | AGATTCACAAATTTATATGT+CGG | + | contig218end:6584-6603 | MsG0080048137.01.T01:intron | 20.0% |
| !! | ATAATTCATTCATATATGGT+TGG | + | contig218end:6864-6883 | MsG0080048137.01.T01:intron | 20.0% |
| !! | CTTCATAATTCATTCATATA+TGG | + | contig218end:6860-6879 | MsG0080048137.01.T01:intron | 20.0% |
| !! | TTAGTTACTAAATTGAAAAC+TGG | + | contig218end:6314-6333 | MsG0080048137.01.T01:intron | 20.0% |
| !!! | CTTTTAATGTTTGAGAATTT+AGG | + | contig218end:6265-6284 | MsG0080048137.01.T01:intron | 20.0% |
| ! | AACAATATACCAATTCCAAT+TGG | - | contig218end:6387-6406 | MsG0080048137.01.T01:intergenic | 25.0% |
| ! | ACAAATACTTGATTGTATTC+AGG | - | contig218end:7033-7052 | MsG0080048137.01.T01:intergenic | 25.0% |
| ! | ATTGGAATTGGTATATTGTT+TGG | + | contig218end:6387-6406 | MsG0080048137.01.T01:intron | 25.0% |
| ! | ATTTCAACAAATGATGCAAA+TGG | - | contig218end:6212-6231 | MsG0080048137.01.T01:intergenic | 25.0% |
| ! | TATGAATGAATTATGAAGCA+CGG | - | contig218end:6858-6877 | MsG0080048137.01.T01:intergenic | 25.0% |
| ! | TTAAATTACAATCAGCATGT+TGG | + | contig218end:6651-6670 | MsG0080048137.01.T01:intron | 25.0% |
| ! | TTCAATGTAATCTTATGTGT+CGG | + | contig218end:6768-6787 | MsG0080048137.01.T01:intron | 25.0% |
| ! | TTTCAACAAATGATGCAAAT+GGG | - | contig218end:6211-6230 | MsG0080048137.01.T01:intergenic | 25.0% |
| !! | GATTGGCTAATTATATCAAA+AGG | + | contig218end:6347-6366 | MsG0080048137.01.T01:intron | 25.0% |
| !! | TTGTCTTGTAATTTCACTTT+TGG | + | contig218end:6962-6981 | MsG0080048137.01.T01:intron | 25.0% |
| !!! | AATTTTCGTTCTTTTTCATG+TGG | + | contig218end:6229-6248 | MsG0080048137.01.T01:intron | 25.0% |
| !!! | GTTTTGATAAGTGGAAATTT+TGG | + | contig218end:6886-6905 | MsG0080048137.01.T01:intron | 25.0% |
| ATTCTGATTCCAATTGGAAT+TGG | + | contig218end:6375-6394 | MsG0080048137.01.T01:intron | 30.0% | |
| TGAAATTACAAGACAAACAG+AGG | - | contig218end:6958-6977 | MsG0080048137.01.T01:intergenic | 30.0% | |
| TGAAGAAGAAAAGAAAGAAG+AGG | - | contig218end:6110-6129 | MsG0080048137.01.T01:intergenic | 30.0% | |
| ! | ACTGGATTGTTAGAATTTTG+AGG | + | contig218end:5974-5993 | MsG0080048137.01.T01:five_prime_UTR | 30.0% |
| !! | AAACTGGTTTGATGATTGAT+TGG | + | contig218end:6330-6349 | MsG0080048137.01.T01:intron | 30.0% |
| !!! | ATATGGTTGGTTTTGATAAG+TGG | + | contig218end:6877-6896 | MsG0080048137.01.T01:intron | 30.0% |
| AAACTCAGCATTTCTTACGA+AGG | - | contig218end:6082-6101 | MsG0080048136.01.T01:intergenic | 35.0% | |
| AATTACAAGACAAACAGAGG+AGG | - | contig218end:6955-6974 | MsG0080048136.01.T01:intergenic | 35.0% | |
| ATTACAAGACAAACAGAGGA+GGG | - | contig218end:6954-6973 | MsG0080048137.01.T01:intergenic | 35.0% | |
| ATTTCTTACGAAGGCGAAAA+TGG | - | contig218end:6073-6092 | MsG0080048137.01.T01:intergenic | 35.0% | |
| GATTACGAGTCTGTTTGAAT+TGG | - | contig218end:6628-6647 | MsG0080048137.01.T01:intergenic | 35.0% | |
| GTAGAGATTCTGATTCCAAT+TGG | + | contig218end:6369-6388 | MsG0080048137.01.T01:intron | 35.0% | |
| TGGTGTATCATGTATGAACA+CGG | + | contig218end:6678-6697 | MsG0080048137.01.T01:intron | 35.0% | |
| ACTGATGAAAACAACTTGCG+AGG | - | contig218end:6554-6573 | MsG0080048137.01.T01:intergenic | 40.0% | |
| CAAGTATTTGTGAAGAAGCC+TGG | + | contig218end:7042-7061 | MsG0080048137.01.T01:CDS | 40.0% | |
| ! | GGAACGAGTCTTTATTGACT+CGG | + | contig218end:6037-6056 | MsG0080048137.01.T01:CDS | 40.0% |
| !!! | ACAATCAGCATGTTGGCTTT+TGG | + | contig218end:6658-6677 | MsG0080048137.01.T01:intron | 40.0% |
| ATCATGTATGAACACGGACG+CGG | + | contig218end:6684-6703 | MsG0080048137.01.T01:intron | 45.0% | |
| CAGAGAGAAGAAGACTAGAC+TGG | + | contig218end:5956-5975 | MsG0080048137.01.T01:five_prime_UTR | 45.0% | |
| GTATTTGTGAAGAAGCCTGG+TGG | + | contig218end:7045-7064 | MsG0080048137.01.T01:CDS | 45.0% | |
| TGCGGACATCAGACATGAAA+CGG | + | contig218end:6708-6727 | MsG0080048137.01.T01:intron | 45.0% | |
| TTCTCAGATTAGGTGTGTCC+CGG | - | contig218end:6831-6850 | MsG0080048137.01.T01:intergenic | 45.0% | |
| !!! | TTATTTTATCAAATTATTAT+TGG | - | contig218end:6743-6762 | MsG0080048137.01.T01:intergenic | 5.0% |
| AAGACAAACAGAGGAGGGAG+TGG | - | contig218end:6949-6968 | MsG0080048136.01.T01:intergenic | 50.0% | |
| AGACAAACAGAGGAGGGAGT+GGG | - | contig218end:6948-6967 | MsG0080048137.01.T01:intergenic | 50.0% | |
| AGAGAACAGCGATGGCTAGA+CGG | + | contig218end:6001-6020 | MsG0080048137.01.T01:exon | 50.0% | |
| GCACGGACACTTCTCAGATT+AGG | - | contig218end:6841-6860 | MsG0080048137.01.T01:intergenic | 50.0% | |
| TTTGTGAAGAAGCCTGGTGG+TGG | + | contig218end:7048-7067 | MsG0080048137.01.T01:CDS | 50.0% | |
| !! | CTTATGTGTCGGTGTCGTGT+TGG | + | contig218end:6779-6798 | MsG0080048137.01.T01:intron | 50.0% |
| ACAAACAGAGGAGGGAGTGG+GGG | - | contig218end:6946-6965 | MsG0080048136.01.T01:intergenic | 55.0% | |
| GACAAACAGAGGAGGGAGTG+GGG | - | contig218end:6947-6966 | MsG0080048137.01.T01:intergenic | 55.0% | |
| GAGGTGAGAGAGAACAGCGA+TGG | + | contig218end:5993-6012 | MsG0080048137.01.T01:exon | 55.0% | |
| GATTAGGTGTGTCCCGGTGT+CGG | - | contig218end:6825-6844 | MsG0080048137.01.T01:intergenic | 55.0% | |
| TATGAACACGGACGCGGATG+CGG | + | contig218end:6690-6709 | MsG0080048137.01.T01:intron | 55.0% | |
| ACCAATGCGTGTCCGACACC+GGG | + | contig218end:6810-6829 | MsG0080048137.01.T01:intron | 60.0% | |
| CAAACAGAGGAGGGAGTGGG+GGG | - | contig218end:6945-6964 | MsG0080048137.01.T01:intergenic | 60.0% | |
| CACCAATGCGTGTCCGACAC+CGG | + | contig218end:6809-6828 | MsG0080048137.01.T01:intron | 60.0% | |
| CTAGACGGCGAGTCGAGACT+CGG | + | contig218end:6016-6035 | MsG0080048137.01.T01:CDS | 60.0% | |
| ! | CAAAGCTCTGGTCCACCACC+AGG | - | contig218end:7063-7082 | MsG0080048137.01.T01:intergenic | 60.0% |
| !! | CTGGTGGTGGACCAGAGCTT+TGG | + | contig218end:7061-7080 | MsG0080048137.01.T01:CDS | 60.0% |
| ! | TCCCGGTGTCGGACACGCAT+TGG | - | contig218end:6814-6833 | MsG0080048137.01.T01:intergenic | 65.0% |
| ACAGAGGAGGGAGTGGGGGG+TGG | - | contig218end:6942-6961 | MsG0080048136.01.T01:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig218end | gene | 5952 | 7092 | 5952 | ID=MsG0080048137.01;Name=MsG0080048137.01 |
| contig218end | mRNA | 5952 | 7092 | 5952 | ID=MsG0080048137.01.T01;Parent=MsG0080048137.01;Name=MsG0080048137.01.T01;_AED=0.33;_eAED=0.33;_QI=60|1|1|1|0|0.5|2|0|71 |
| contig218end | exon | 5952 | 6155 | 5952 | ID=MsG0080048137.01.T01:exon:36057;Parent=MsG0080048137.01.T01 |
| contig218end | exon | 7021 | 7092 | 7021 | ID=MsG0080048137.01.T01:exon:36058;Parent=MsG0080048137.01.T01 |
| contig218end | five_prime_UTR | 5952 | 6011 | 5952 | ID=MsG0080048137.01.T01:five_prime_utr;Parent=MsG0080048137.01.T01 |
| contig218end | CDS | 6012 | 6155 | 6012 | ID=MsG0080048137.01.T01:cds;Parent=MsG0080048137.01.T01 |
| contig218end | CDS | 7021 | 7092 | 7021 | ID=MsG0080048137.01.T01:cds;Parent=MsG0080048137.01.T01 |
| Gene Sequence |
| Protein sequence |
