AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006477.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006477.01.T01 MTR_2g009275 77.143 105 17 2 1 105 1 98 4.89e-26 93.2
MsG0280006477.01.T01 MTR_2g009150 45.714 105 42 4 1 104 1 91 2.91e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCTGTTGCTGCTACTGC+TGG 0.364799 2:+2536665 MsG0280006477.01.T01:CDS
GTCTCTCCTTCTTCCATCTC+TGG 0.367654 2:+2536605 MsG0280006477.01.T01:CDS
TTTCCTTGGCGACTGTGATT+TGG 0.378125 2:-2536507 MsG0280006478.01.T01:CDS
GGTGAAGTAGCGGATGGTTC+AGG 0.402991 2:-2536578 MsG0280006478.01.T01:CDS
GTTGTTGAGTTCTACAACAA+TGG 0.418465 2:+2536460 MsG0280006477.01.T01:CDS
TACAACAATGGCTCAATCTC+CGG 0.448075 2:+2536472 MsG0280006477.01.T01:CDS
GGAGGAGAATTTCCAGTGGC+AGG 0.448322 2:-2536557 MsG0280006478.01.T01:CDS
GATGGGGAGATTGCTTTCCT+TGG 0.456249 2:-2536521 MsG0280006478.01.T01:CDS
CAGGAGAAGACGCCGGTGAT+GGG 0.475687 2:-2536538 MsG0280006478.01.T01:CDS
GCAGGAGAAGACGCCGGTGA+TGG 0.484603 2:-2536539 MsG0280006478.01.T01:CDS
ACGGCACTGGAAGCAGGTCC+TGG 0.494599 2:-2536644 MsG0280006478.01.T01:CDS
GAACATTGCTCTTATGATTG+TGG 0.500197 2:+2536430 MsG0280006477.01.T01:CDS
ACAGAGACGGCACTGGAAGC+AGG 0.508633 2:-2536650 MsG0280006478.01.T01:CDS
GGTCCTGGTGCTTCAGATGG+TGG 0.509452 2:-2536629 MsG0280006478.01.T01:CDS
AGCAGCAACAGAGACGGCAC+TGG 0.519764 2:-2536657 MsG0280006478.01.T01:CDS
CCGGCGTCTTCTCCTGCCAC+TGG 0.519854 2:+2536545 MsG0280006477.01.T01:CDS
GCAGGTCCTGGTGCTTCAGA+TGG 0.523119 2:-2536632 MsG0280006478.01.T01:CDS
CTTGGCGACTGTGATTTGGT+TGG 0.533471 2:-2536503 MsG0280006478.01.T01:CDS
GGTGGACCAGAGATGGAAGA+AGG 0.548296 2:-2536611 MsG0280006478.01.T01:CDS
AGGAGAAGACGCCGGTGATG+GGG 0.563301 2:-2536537 MsG0280006478.01.T01:CDS
GAAAGCAATCTCCCCATCAC+CGG 0.563344 2:+2536526 MsG0280006477.01.T01:CDS
TGAACCATCCGCTACTTCAC+CGG 0.570119 2:+2536580 MsG0280006477.01.T01:CDS
TTCAGATGGTGGACCAGAGA+TGG 0.580542 2:-2536618 MsG0280006478.01.T01:CDS
ATGGAAGAAGGAGAGACAGC+CGG 0.582727 2:-2536599 MsG0280006478.01.T01:CDS
AGAGACAGCCGGTGAAGTAG+CGG 0.584069 2:-2536588 MsG0280006478.01.T01:CDS
CAACCAAATCACAGTCGCCA+AGG 0.590381 2:+2536504 MsG0280006477.01.T01:CDS
GATTTGGTTGGCGATGAAGC+CGG 0.598616 2:-2536491 MsG0280006478.01.T01:CDS
GGTCCACCATCTGAAGCACC+AGG 0.613375 2:+2536626 MsG0280006477.01.T01:CDS
AGCAGTAGCAGCAACAGAGA+CGG 0.613692 2:-2536663 MsG0280006478.01.T01:CDS
GAAGTAGCGGATGGTTCAGG+AGG 0.667793 2:-2536575 MsG0280006478.01.T01:CDS
ACAGCCGGTGAAGTAGCGGA+TGG 0.678585 2:-2536584 MsG0280006478.01.T01:CDS
TTCAGGAGGAGAATTTCCAG+TGG 0.715472 2:-2536561 MsG0280006478.01.T01:CDS
CCAGTGGCAGGAGAAGACGC+CGG 0.748622 2:-2536545 MsG0280006478.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GAACATTGCTCTTATGATTG+TGG + Chr2:2536430-2536449 MsG0280006477.01.T01:CDS 35.0%
GTTGTTGAGTTCTACAACAA+TGG + Chr2:2536460-2536479 MsG0280006477.01.T01:CDS 35.0%
!!! TGCTATTGTCATTTTCGTAG+CGG + Chr2:2536694-2536713 MsG0280006477.01.T01:CDS 35.0%
TACAACAATGGCTCAATCTC+CGG + Chr2:2536472-2536491 MsG0280006477.01.T01:CDS 40.0%
TTCAGGAGGAGAATTTCCAG+TGG - Chr2:2536564-2536583 MsG0280006478.01.T01:CDS 45.0%
TTTCCTTGGCGACTGTGATT+TGG - Chr2:2536510-2536529 MsG0280006478.01.T01:CDS 45.0%
AGCAGTAGCAGCAACAGAGA+CGG - Chr2:2536666-2536685 MsG0280006478.01.T01:CDS 50.0%
ATGGAAGAAGGAGAGACAGC+CGG - Chr2:2536602-2536621 MsG0280006478.01.T01:CDS 50.0%
CAACCAAATCACAGTCGCCA+AGG + Chr2:2536504-2536523 MsG0280006477.01.T01:CDS 50.0%
GAAAGCAATCTCCCCATCAC+CGG + Chr2:2536526-2536545 MsG0280006477.01.T01:CDS 50.0%
GATGGGGAGATTGCTTTCCT+TGG - Chr2:2536524-2536543 MsG0280006478.01.T01:CDS 50.0%
GTCTCTCCTTCTTCCATCTC+TGG + Chr2:2536605-2536624 MsG0280006477.01.T01:CDS 50.0%
TGAACCATCCGCTACTTCAC+CGG + Chr2:2536580-2536599 MsG0280006477.01.T01:CDS 50.0%
! GATTTGGTTGGCGATGAAGC+CGG - Chr2:2536494-2536513 MsG0280006478.01.T01:CDS 50.0%
!! CTTGGCGACTGTGATTTGGT+TGG - Chr2:2536506-2536525 MsG0280006478.01.T01:CDS 50.0%
!! TTCAGATGGTGGACCAGAGA+TGG - Chr2:2536621-2536640 MsG0280006478.01.T01:CDS 50.0%
AGAGACAGCCGGTGAAGTAG+CGG - Chr2:2536591-2536610 MsG0280006478.01.T01:CDS 55.0%
GGAGGAGAATTTCCAGTGGC+AGG - Chr2:2536560-2536579 MsG0280006478.01.T01:CDS 55.0%
GGTGGACCAGAGATGGAAGA+AGG - Chr2:2536614-2536633 MsG0280006478.01.T01:CDS 55.0%
GTCTCTGTTGCTGCTACTGC+TGG + Chr2:2536665-2536684 MsG0280006477.01.T01:CDS 55.0%
!! GAAGTAGCGGATGGTTCAGG+AGG - Chr2:2536578-2536597 MsG0280006478.01.T01:CDS 55.0%
!! GGTGAAGTAGCGGATGGTTC+AGG - Chr2:2536581-2536600 MsG0280006478.01.T01:CDS 55.0%
AGCAGCAACAGAGACGGCAC+TGG - Chr2:2536660-2536679 MsG0280006478.01.T01:CDS 60.0%
AGGAGAAGACGCCGGTGATG+GGG - Chr2:2536540-2536559 MsG0280006478.01.T01:CDS 60.0%
CAGGAGAAGACGCCGGTGAT+GGG - Chr2:2536541-2536560 MsG0280006478.01.T01:CDS 60.0%
GCAGGTCCTGGTGCTTCAGA+TGG - Chr2:2536635-2536654 MsG0280006478.01.T01:CDS 60.0%
GGTCCTGGTGCTTCAGATGG+TGG - Chr2:2536632-2536651 MsG0280006478.01.T01:CDS 60.0%
! ACAGAGACGGCACTGGAAGC+AGG - Chr2:2536653-2536672 MsG0280006478.01.T01:CDS 60.0%
! ACAGCCGGTGAAGTAGCGGA+TGG - Chr2:2536587-2536606 MsG0280006478.01.T01:CDS 60.0%
! GGTCCACCATCTGAAGCACC+AGG + Chr2:2536626-2536645 MsG0280006477.01.T01:CDS 60.0%
CCAGTGGCAGGAGAAGACGC+CGG - Chr2:2536548-2536567 MsG0280006478.01.T01:CDS 65.0%
GCAGGAGAAGACGCCGGTGA+TGG - Chr2:2536542-2536561 MsG0280006478.01.T01:CDS 65.0%
! ACGGCACTGGAAGCAGGTCC+TGG - Chr2:2536647-2536666 MsG0280006478.01.T01:CDS 65.0%
CCGGCGTCTTCTCCTGCCAC+TGG + Chr2:2536545-2536564 MsG0280006477.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 2536413 2536730 2536413 ID=MsG0280006477.01;Name=MsG0280006477.01
Chr2 mRNA 2536413 2536730 2536413 ID=MsG0280006477.01.T01;Parent=MsG0280006477.01;Name=MsG0280006477.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|105
Chr2 exon 2536413 2536730 2536413 ID=MsG0280006477.01.T01:exon:19223;Parent=MsG0280006477.01.T01
Chr2 CDS 2536413 2536730 2536413 ID=MsG0280006477.01.T01:cds;Parent=MsG0280006477.01.T01
Gene Sequence

>MsG0280006477.01.T01

ATGGGTCGCTTCAACTTGAACATTGCTCTTATGATTGTGGCTGCATTGTTGTTGAGTTCTACAACAATGGCTCAATCTCCGGCTTCATCGCCAACCAAATCACAGTCGCCAAGGAAAGCAATCTCCCCATCACCGGCGTCTTCTCCTGCCACTGGAAATTCTCCTCCTGAACCATCCGCTACTTCACCGGCTGTCTCTCCTTCTTCCATCTCTGGTCCACCATCTGAAGCACCAGGACCTGCTTCCAGTGCCGTCTCTGTTGCTGCTACTGCTGGATCTGCTGCTATTGTCATTTTCGTAGCGGCTTTCATCATGTAG

Protein sequence

>MsG0280006477.01.T01

MGRFNLNIALMIVAALLLSSTTMAQSPASSPTKSQSPRKAISPSPASSPATGNSPPEPSATSPAVSPSSISGPPSEAPGPASSAVSVAATAGSAAIVIFVAAFIM*