Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0880045552.01 | 0.814666 | 1.371438e-51 | 9.258833e-49 |
| MsG0080048311.01 | MsG0880045552.01 | 0.858578 | 7.485080e-63 | 1.919191e-59 |
| MsG0080048781.01 | MsG0880045552.01 | 0.861049 | 1.348143e-63 | 3.763200e-60 |
| MsG0080049140.01 | MsG0880045552.01 | 0.889719 | 1.882423e-73 | 1.536467e-69 |
| MsG0180000348.01 | MsG0880045552.01 | 0.814577 | 1.435183e-51 | 9.666194e-49 |
| MsG0180000373.01 | MsG0880045552.01 | 0.802558 | 5.298570e-49 | 2.603355e-46 |
| MsG0180002421.01 | MsG0880045552.01 | 0.837330 | 5.541225e-57 | 7.173773e-54 |
| MsG0180003427.01 | MsG0880045552.01 | 0.817241 | 3.651538e-52 | 2.644883e-49 |
| MsG0180003796.01 | MsG0880045552.01 | 0.837971 | 3.792273e-57 | 5.008055e-54 |
| MsG0180004099.01 | MsG0880045552.01 | 0.828505 | 8.659069e-55 | 8.623936e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0180000639.01 | MsG0880045552.01 | PPI |
| MsG0280007787.01 | MsG0880045552.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045552.01.T01 | MTR_8g074000 | 74.104 | 251 | 12 | 1 | 161 | 358 | 184 | 434 | 3.86e-124 | 364 |
| MsG0880045552.01.T01 | MTR_8g074000 | 53.435 | 131 | 12 | 2 | 49 | 163 | 1 | 98 | 6.46e-31 | 122 |
| MsG0880045552.01.T01 | MTR_5g023790 | 62.651 | 249 | 40 | 1 | 161 | 356 | 180 | 428 | 3.81e-101 | 305 |
| MsG0880045552.01.T01 | MTR_5g023790 | 59.406 | 202 | 29 | 1 | 161 | 309 | 180 | 381 | 5.75e-70 | 224 |
| MsG0880045552.01.T01 | MTR_5g023790 | 38.843 | 121 | 28 | 5 | 54 | 163 | 6 | 91 | 2.95e-12 | 67.8 |
| MsG0880045552.01.T01 | MTR_5g023790 | 79.817 | 109 | 22 | 0 | 161 | 269 | 180 | 288 | 6.55e-51 | 174 |
| MsG0880045552.01.T01 | MTR_5g023790 | 38.843 | 121 | 28 | 5 | 54 | 163 | 6 | 91 | 2.77e-12 | 67.4 |
| MsG0880045552.01.T01 | MTR_7g089080 | 36.709 | 237 | 91 | 4 | 175 | 352 | 220 | 456 | 1.49e-36 | 141 |
| MsG0880045552.01.T01 | MTR_5g088980 | 34.498 | 229 | 95 | 2 | 175 | 348 | 186 | 414 | 5.35e-36 | 135 |
| MsG0880045552.01.T01 | MTR_1g011470 | 48.913 | 92 | 47 | 0 | 178 | 269 | 187 | 278 | 4.36e-24 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045552.01.T01 | AT2G17620 | 43.223 | 391 | 115 | 8 | 67 | 352 | 35 | 423 | 4.86e-78 | 246 |
| MsG0880045552.01.T01 | AT1G20610 | 51.948 | 231 | 58 | 1 | 175 | 352 | 199 | 429 | 7.69e-77 | 243 |
| MsG0880045552.01.T01 | AT1G76310 | 51.724 | 232 | 58 | 2 | 175 | 352 | 199 | 430 | 2.36e-73 | 234 |
| MsG0880045552.01.T01 | AT4G35620 | 52.400 | 250 | 65 | 2 | 161 | 356 | 180 | 429 | 2.12e-70 | 226 |
| MsG0880045552.01.T01 | AT4G35620 | 53.368 | 193 | 37 | 1 | 170 | 309 | 189 | 381 | 9.06e-52 | 176 |
| MsG0880045552.01.T01 | AT1G76310 | 48.148 | 189 | 44 | 2 | 175 | 309 | 199 | 387 | 2.23e-49 | 170 |
| MsG0880045552.01.T01 | AT3G11520 | 38.057 | 247 | 99 | 2 | 161 | 354 | 167 | 412 | 1.03e-46 | 164 |
| MsG0880045552.01.T01 | AT5G06150 | 36.797 | 231 | 93 | 1 | 175 | 352 | 203 | 433 | 3.69e-45 | 160 |
| MsG0880045552.01.T01 | AT1G20590 | 45.882 | 170 | 39 | 1 | 213 | 329 | 65 | 234 | 3.70e-44 | 153 |
| MsG0880045552.01.T01 | AT1G76310 | 73.958 | 96 | 24 | 1 | 175 | 269 | 199 | 294 | 5.73e-40 | 144 |
Find 83 sgRNAs with CRISPR-Local
Find 545 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAACTTGACCTCAATGAG+AGG | 0.630153 | 8:+60990125 | MsG0880045552.01.T01:CDS |
| AAGAGAAACTACCTCAGCAT+AGG | 0.594491 | 8:-60988829 | None:intergenic |
| AAGGAAAGACATTCTCGAAA+TGG | 0.454782 | 8:+60990471 | MsG0880045552.01.T01:CDS |
| ACAGCTCAATGCACTGTCAG+AGG | 0.636227 | 8:+60990872 | MsG0880045552.01.T01:CDS |
| ACAGGAGTGCATAGGAAGTA+TGG | 0.389149 | 8:+60991206 | MsG0880045552.01.T01:CDS |
| ACCCTCTTGTGTACAACACA+AGG | 0.585319 | 8:-60988012 | None:intergenic |
| ACCCTTGTGTTGTACACAAG+AGG | 0.563360 | 8:+60988010 | MsG0880045552.01.T01:CDS |
| ACTAGTTGACTGGCTGATTG+AGG | 0.576580 | 8:+60990159 | MsG0880045552.01.T01:CDS |
| ACTGGAACAAGACATGCGAG+TGG | 0.734063 | 8:+60990903 | MsG0880045552.01.T01:CDS |
| AGAAAGAAACTTCAGCTGGT+TGG | 0.565294 | 8:+60990358 | MsG0880045552.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATTTAATGCCATAGACCA+TGG | + | Chr8:60988622-60988641 | MsG0880045552.01.T01:intron | 30.0% | |
| AACAATTCGCGTTACTTATT+TGG | + | Chr8:60983392-60983411 | MsG0880045552.01.T01:intron | 30.0% | |
| AAGAAGAAAACGAGAATAAC+GGG | - | Chr8:60987515-60987534 | None:intergenic | 30.0% | |
| AATAAGTAACGCGAATTGTT+TGG | - | Chr8:60983392-60983411 | None:intergenic | 30.0% | |
| AATCCCCTCTATTTAATTGT+TGG | + | Chr8:60983514-60983533 | MsG0880045552.01.T01:intron | 30.0% | |
| ACATTGAATGATATCGTTCA+AGG | - | Chr8:60985817-60985836 | None:intergenic | 30.0% | |
| AGATCATAGATATTCGAATC+AGG | - | Chr8:60989676-60989695 | None:intergenic | 30.0% | |
| AGTTATGCCAGAAAATTTCA+AGG | + | Chr8:60987731-60987750 | MsG0880045552.01.T01:CDS | 30.0% | |
| ATAAAAAATCGTTCAGGGAA+AGG | - | Chr8:60985771-60985790 | None:intergenic | 30.0% | |
| ATATAGAAAGATGCAACCTT+AGG | + | Chr8:60987059-60987078 | MsG0880045552.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 60981702 | 60991301 | 60981702 | ID=MsG0880045552.01;Name=MsG0880045552.01 |
| Chr8 | mRNA | 60981702 | 60991301 | 60981702 | ID=MsG0880045552.01.T01;Parent=MsG0880045552.01;Name=MsG0880045552.01.T01;_AED=0.48;_eAED=0.60;_QI=0|0|0|1|0.33|0.4|10|0|358 |
| Chr8 | exon | 60981702 | 60981828 | 60981702 | ID=MsG0880045552.01.T01:exon:23327;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60987679 | 60987752 | 60987679 | ID=MsG0880045552.01.T01:exon:23328;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60987958 | 60988043 | 60987958 | ID=MsG0880045552.01.T01:exon:23329;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60988175 | 60988231 | 60988175 | ID=MsG0880045552.01.T01:exon:23330;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60988378 | 60988438 | 60988378 | ID=MsG0880045552.01.T01:exon:23331;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60988756 | 60988852 | 60988756 | ID=MsG0880045552.01.T01:exon:23332;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60990098 | 60990180 | 60990098 | ID=MsG0880045552.01.T01:exon:23333;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60990271 | 60990492 | 60990271 | ID=MsG0880045552.01.T01:exon:23334;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60990836 | 60990954 | 60990836 | ID=MsG0880045552.01.T01:exon:23335;Parent=MsG0880045552.01.T01 |
| Chr8 | exon | 60991151 | 60991301 | 60991151 | ID=MsG0880045552.01.T01:exon:23336;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60981702 | 60981828 | 60981702 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60987679 | 60987752 | 60987679 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60987958 | 60988043 | 60987958 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60988175 | 60988231 | 60988175 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60988378 | 60988438 | 60988378 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60988756 | 60988852 | 60988756 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60990098 | 60990180 | 60990098 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60990271 | 60990492 | 60990271 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60990836 | 60990954 | 60990836 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Chr8 | CDS | 60991151 | 60991301 | 60991151 | ID=MsG0880045552.01.T01:cds;Parent=MsG0880045552.01.T01 |
| Gene Sequence |
>MsG0880045552.01.T01 ATGGATATTTCATGGTTGGAGGCTCACGTGGTGGCGACGGTTGAGATTGTGATGGCGGCGTCGTTGTGGATACTACATGGTGAAGGGTTTAACGACGGTGGTGACGATGACAAGTTTAATGGCGGTGGAAAGAACAACAACAACATGGTGAATACTTCTGAAGAGAACAATTCCAACGCAGTTATGCCAGAAAATTTCAAGAACAGAAGAGCTTTAGGAGGGATCAATCAGAATTTTGTGCATGGTCGACCTTACCCTTGTGTTGTACACAAGAGGGTGTTGTCTGAAAAACATGAGATTTGTGAAAAGAACAGGCTGATCTGGGACATAGACCCCATCACAAGATTGTTGCTGGTTGAGGATGATGCTTTTTTTTGCTTTGTTTTTGTTCTTGTTAAGAATTATGAATTAATGAAACTTTATGTTGATGTATTGTTAATTAGGAGGTTTGCTGCACAAATTGCTGGCTCACAGCAATCCTATGCTGAGGTAGTTTCTCTTTTATCCGTTGGCTTGTGTTTGATGGATGAACAACTTGACCTCAATGAGAGGATGAGGGCTATACTAGTTGACTGGCTGATTGAGGTTCATGACAAATTCGACCTCATGCAAGAGACACTGTTTCTTACAGTTAATCTCATAGACAGATTCTTGGCTAAGCAGAATGTTGTAAGAAAGAAACTTCAGCTGGTTGGTCTTGTTGCCATGCTTTTGGCATGCAAGTATGAGGAAGTTTCTGTGCCTGTCGTTTCGGATCTGATCCATATAGCTGACAGAGCTTACACAAGGAAAGACATTCTCGAAATGTTTCCACCATCCTTGGTTGCTGCTGCTGCAGTCTACACAGCTCAATGCACTGTCAGAGGTTTCAAACACTGGAACAAGACATGCGAGTGGCACACAAACTACTCAGAAGATCAGCTGTTAGAGTGCTCTATGCTGATGGTTGGATTCCACCAGAAAGCCGGAGCCGGGAAACTGACAGGAGTGCATAGGAAGTATGGCTCAGCAAAGTTTAGCTTCACTGCTAAATGTGAACCAGCATGTTTTCTTCTGGAGAACAAGAACCAGCCATAG |
| Protein sequence |
>MsG0880045552.01.T01 MDISWLEAHVVATVEIVMAASLWILHGEGFNDGGDDDKFNGGGKNNNNMVNTSEENNSNAVMPENFKNRRALGGINQNFVHGRPYPCVVHKRVLSEKHEICEKNRLIWDIDPITRLLLVEDDAFFCFVFVLVKNYELMKLYVDVLLIRRFAAQIAGSQQSYAEVVSLLSVGLCLMDEQLDLNERMRAILVDWLIEVHDKFDLMQETLFLTVNLIDRFLAKQNVVRKKLQLVGLVAMLLACKYEEVSVPVVSDLIHIADRAYTRKDILEMFPPSLVAAAAVYTAQCTVRGFKHWNKTCEWHTNYSEDQLLECSMLMVGFHQKAGAGKLTGVHRKYGSAKFSFTAKCEPACFLLENKNQP* |
