AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027135.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027135.01.T01 MTR_6g068860 98.089 157 3 0 1 157 1 157 1.40e-110 311
MsG0580027135.01.T01 MTR_2g049340 75.524 143 35 0 1 143 6 148 8.72e-71 210
MsG0580027135.01.T01 MTR_4g117600 45.985 137 74 0 16 152 21 157 3.91e-35 120
MsG0580027135.01.T01 MTR_1g094035 42.857 154 73 5 2 154 6 145 3.95e-27 100
MsG0580027135.01.T01 MTR_4g117600 46.939 98 52 0 16 113 21 118 3.77e-23 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027135.01.T01 AT5G46530 71.812 149 42 0 1 149 6 154 1.84e-76 225
MsG0580027135.01.T01 AT1G29520 78.523 149 32 0 1 149 6 154 1.71e-75 222
MsG0580027135.01.T01 AT5G18970 43.506 154 87 0 2 155 7 160 1.10e-37 127
MsG0580027135.01.T01 AT1G04560 43.590 156 74 4 2 156 7 149 8.39e-31 110

Find 28 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCCCATTGGGAAGAAAA+TGG 0.240357 5:+54220648 None:intergenic
TGCATGTATGTCATTGTTCT+AGG 0.248721 5:-54220924 MsG0580027135.01.T01:CDS
CTCTACATAGTTGCAATTCA+TGG 0.294119 5:-54219805 MsG0580027135.01.T01:CDS
GGTGCTGCATCGACAATTTC+AGG 0.318250 5:-54220577 MsG0580027135.01.T01:CDS
TGCTGCTTCAATTGCTACTT+TGG 0.334645 5:-54220512 MsG0580027135.01.T01:CDS
CCAATGGGAAATGCTTCAAC+TGG 0.374176 5:-54220637 MsG0580027135.01.T01:CDS
CTTCAATTGCTACTTTGGCT+TGG 0.384442 5:-54220507 MsG0580027135.01.T01:CDS
GACTCTTACACTGCTTGCCA+TGG 0.388463 5:-54220485 MsG0580027135.01.T01:CDS
GTTACGATTTCTTTGTTAGC+TGG 0.398205 5:-54220607 MsG0580027135.01.T01:CDS
TCTTTGTTAGCTGGAGTAGT+TGG 0.415192 5:-54220598 MsG0580027135.01.T01:CDS
TTGTTCTAGGCATTGGTGCA+TGG 0.424644 5:-54220911 MsG0580027135.01.T01:CDS
CAGTTGAAGCATTTCCCATT+GGG 0.433142 5:+54220638 None:intergenic
TGAGCTGGAAGATTTAATTC+CGG 0.439380 5:+54220679 None:intergenic
TGTTCTAGGCATTGGTGCAT+GGG 0.449144 5:-54220910 MsG0580027135.01.T01:CDS
ACTCTTACACTGCTTGCCAT+GGG 0.453490 5:-54220484 MsG0580027135.01.T01:CDS
TTTCCCATTGGGAAGAAAAT+GGG 0.454684 5:+54220649 None:intergenic
GAGTCAAACATATCTGTTCA+TGG 0.465531 5:-54220555 MsG0580027135.01.T01:CDS
TCAAATTAGAAATGCACGTC+TGG 0.465623 5:-54219958 MsG0580027135.01.T01:intron
CCAGTTGAAGCATTTCCCAT+TGG 0.492597 5:+54220637 None:intergenic
GATAATTATGAAAGCCTCCA+TGG 0.495765 5:+54219846 None:intergenic
TATGTCATTGTTCTAGGCAT+TGG 0.506725 5:-54220918 MsG0580027135.01.T01:CDS
GTCCATAGCAACACTTCTCT+TGG 0.510981 5:-54220958 MsG0580027135.01.T01:CDS
CACCAAGAGAAGTGTTGCTA+TGG 0.525202 5:+54220956 None:intergenic
TTAACTTCTGCAGATTTGCA+TGG 0.530112 5:-54219995 MsG0580027135.01.T01:intron
ATGAATAGAGCAATAGATCA+TGG 0.550315 5:-54220885 MsG0580027135.01.T01:CDS
AGAGAAGTTGTGTAACTGAA+AGG 0.568727 5:+54219824 None:intergenic
GTAGCAATTGAAGCAGCAGA+TGG 0.611432 5:+54220517 None:intergenic
CTCTTACACTGCTTGCCATG+GGG 0.615745 5:-54220483 MsG0580027135.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAACTATAAAAATCAA+AGG - Chr5:54220456-54220475 MsG0580027135.01.T01:intron 10.0%
!!! TTTTTATTACTATTATTTAG+AGG + Chr5:54220323-54220342 None:intergenic 10.0%
!!! GAATCATTTAATAATTTTCT+TGG - Chr5:54220818-54220837 MsG0580027135.01.T01:intron 15.0%
!!! GTTTTAATTGAAAACAAATT+TGG + Chr5:54220678-54220697 None:intergenic 15.0%
!! ATTACTATTATTTAGAGGTA+TGG + Chr5:54220318-54220337 None:intergenic 20.0%
!!! ATTTTGTTATGAAATTATGC+AGG - Chr5:54220037-54220056 MsG0580027135.01.T01:intron 20.0%
!!! TTCTGACATTCTAAAATATT+TGG + Chr5:54219923-54219942 None:intergenic 20.0%
! CAATGAATTTCGTAAAATCA+TGG - Chr5:54220591-54220610 MsG0580027135.01.T01:CDS 25.0%
! TTAGAATGTCAGAAAAACAA+TGG - Chr5:54219929-54219948 MsG0580027135.01.T01:intron 25.0%
!! TGTAACTTTTGTCAAAATCA+CGG - Chr5:54220644-54220663 MsG0580027135.01.T01:CDS 25.0%
!! TTGATTTTATCCATTCCTAT+AGG - Chr5:54220387-54220406 MsG0580027135.01.T01:intron 25.0%
!!! ATAGATCATGGTTTTATCAT+AGG - Chr5:54219867-54219886 MsG0580027135.01.T01:intron 25.0%
ATGAATAGAGCAATAGATCA+TGG - Chr5:54219855-54219874 MsG0580027135.01.T01:intron 30.0%
ATTTGGCAAATGTGAAAGAT+AGG + Chr5:54219906-54219925 None:intergenic 30.0%
TAAGTCTATCAATCACCTAT+AGG + Chr5:54220405-54220424 None:intergenic 30.0%
! AAATCAAAGGAGCGTATTAA+AGG - Chr5:54220469-54220488 MsG0580027135.01.T01:intron 30.0%
! AATCAAAGGAGCGTATTAAA+GGG - Chr5:54220470-54220489 MsG0580027135.01.T01:intron 30.0%
!!! GTATGCTGAAGTTTTAATTC+CGG + Chr5:54220015-54220034 None:intergenic 30.0%
!!! TCTATTTTTGCAGAAAACCA+TGG - Chr5:54220877-54220896 MsG0580027135.01.T01:CDS 30.0%
AAAGAGAGAAATTTACCCCA+TGG + Chr5:54220275-54220294 None:intergenic 35.0%
AGAGAAGTTGTGTAACTGAA+AGG + Chr5:54220919-54220938 None:intergenic 35.0%
ATTTCCCATTGGGAAGAAAA+TGG + Chr5:54220095-54220114 None:intergenic 35.0%
CTATCAATCACCTATAGGAA+TGG + Chr5:54220400-54220419 None:intergenic 35.0%
CTCTACATAGTTGCAATTCA+TGG - Chr5:54220935-54220954 MsG0580027135.01.T01:CDS 35.0%
GAGTCAAACATATCTGTTCA+TGG - Chr5:54220185-54220204 MsG0580027135.01.T01:intron 35.0%
GATAATTATGAAAGCCTCCA+TGG + Chr5:54220897-54220916 None:intergenic 35.0%
GTTACGATTTCTTTGTTAGC+TGG - Chr5:54220133-54220152 MsG0580027135.01.T01:intron 35.0%
GTTATGAAATTATGCAGGTC+CGG - Chr5:54220042-54220061 MsG0580027135.01.T01:intron 35.0%
TCAAATTAGAAATGCACGTC+TGG - Chr5:54220782-54220801 MsG0580027135.01.T01:intron 35.0%
TGAGCTGGAAGATTTAATTC+CGG + Chr5:54220064-54220083 None:intergenic 35.0%
TGCATGTATGTCATTGTTCT+AGG - Chr5:54219816-54219835 MsG0580027135.01.T01:CDS 35.0%
TTAACTTCTGCAGATTTGCA+TGG - Chr5:54220745-54220764 MsG0580027135.01.T01:intron 35.0%
TTTCCCATTGGGAAGAAAAT+GGG + Chr5:54220094-54220113 None:intergenic 35.0%
! ATTTTTGCAGAAAACCATGG+AGG - Chr5:54220880-54220899 MsG0580027135.01.T01:CDS 35.0%
! TATGTCATTGTTCTAGGCAT+TGG - Chr5:54219822-54219841 MsG0580027135.01.T01:CDS 35.0%
! TGCTCTTCTTTTATCTTACC+AGG - Chr5:54220552-54220571 MsG0580027135.01.T01:CDS 35.0%
AAAATGGGCGAAAAATGAGC+TGG + Chr5:54220079-54220098 None:intergenic 40.0%
AACGTGTGAGTTAGGTTTAC+CGG - Chr5:54219988-54220007 MsG0580027135.01.T01:intron 40.0%
TATCTTACCAGGTGCATGTT+TGG - Chr5:54220563-54220582 MsG0580027135.01.T01:CDS 40.0%
TCTTTGTTAGCTGGAGTAGT+TGG - Chr5:54220142-54220161 MsG0580027135.01.T01:intron 40.0%
! CAGTTGAAGCATTTCCCATT+GGG + Chr5:54220105-54220124 None:intergenic 40.0%
! TGCTGCTTCAATTGCTACTT+TGG - Chr5:54220228-54220247 MsG0580027135.01.T01:intron 40.0%
!! CTTCAATTGCTACTTTGGCT+TGG - Chr5:54220233-54220252 MsG0580027135.01.T01:intron 40.0%
!!! CTGAAGTTTTAATTCCGGTC+CGG + Chr5:54220010-54220029 None:intergenic 40.0%
ACTCTTACACTGCTTGCCAT+GGG - Chr5:54220256-54220275 MsG0580027135.01.T01:intron 45.0%
CCAATGGGAAATGCTTCAAC+TGG - Chr5:54220103-54220122 MsG0580027135.01.T01:intron 45.0%
GTAGCAATTGAAGCAGCAGA+TGG + Chr5:54220226-54220245 None:intergenic 45.0%
GTCCATAGCAACACTTCTCT+TGG - Chr5:54219782-54219801 MsG0580027135.01.T01:CDS 45.0%
TGCGTAGTAACGTGTGAGTT+AGG - Chr5:54219980-54219999 MsG0580027135.01.T01:CDS 45.0%
TGTCAATCCAAACATGCACC+TGG + Chr5:54220573-54220592 None:intergenic 45.0%
! CCAGTTGAAGCATTTCCCAT+TGG + Chr5:54220106-54220125 None:intergenic 45.0%
! TCGCCCATTTTCTTCCCAAT+GGG - Chr5:54220088-54220107 MsG0580027135.01.T01:intron 45.0%
! TTCGCCCATTTTCTTCCCAA+TGG - Chr5:54220087-54220106 MsG0580027135.01.T01:intron 45.0%
!! CACCAAGAGAAGTGTTGCTA+TGG + Chr5:54219787-54219806 None:intergenic 45.0%
!! TGTTCTAGGCATTGGTGCAT+GGG - Chr5:54219830-54219849 MsG0580027135.01.T01:CDS 45.0%
!! TTGTTCTAGGCATTGGTGCA+TGG - Chr5:54219829-54219848 MsG0580027135.01.T01:CDS 45.0%
CTCTTACACTGCTTGCCATG+GGG - Chr5:54220257-54220276 MsG0580027135.01.T01:intron 50.0%
GACTCTTACACTGCTTGCCA+TGG - Chr5:54220255-54220274 MsG0580027135.01.T01:intron 50.0%
GTGAGTTAGGTTTACCGGAC+CGG - Chr5:54219993-54220012 MsG0580027135.01.T01:intron 50.0%
! GGTGCTGCATCGACAATTTC+AGG - Chr5:54220163-54220182 MsG0580027135.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 54219777 54220985 54219777 ID=MsG0580027135.01;Name=MsG0580027135.01
Chr5 mRNA 54219777 54220985 54219777 ID=MsG0580027135.01.T01;Parent=MsG0580027135.01;Name=MsG0580027135.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|1|1|4|0|157
Chr5 exon 54220874 54220985 54220874 ID=MsG0580027135.01.T01:exon:40555;Parent=MsG0580027135.01.T01
Chr5 exon 54220484 54220703 54220484 ID=MsG0580027135.01.T01:exon:40556;Parent=MsG0580027135.01.T01
Chr5 exon 54219959 54220004 54219959 ID=MsG0580027135.01.T01:exon:40557;Parent=MsG0580027135.01.T01
Chr5 exon 54219777 54219872 54219777 ID=MsG0580027135.01.T01:exon:40558;Parent=MsG0580027135.01.T01
Chr5 CDS 54220874 54220985 54220874 ID=MsG0580027135.01.T01:cds;Parent=MsG0580027135.01.T01
Chr5 CDS 54220484 54220703 54220484 ID=MsG0580027135.01.T01:cds;Parent=MsG0580027135.01.T01
Chr5 CDS 54219959 54220004 54219959 ID=MsG0580027135.01.T01:cds;Parent=MsG0580027135.01.T01
Chr5 CDS 54219777 54219872 54219777 ID=MsG0580027135.01.T01:cds;Parent=MsG0580027135.01.T01
Gene Sequence

>MsG0580027135.01.T01

ATGAAGTCCATAGCAACACTTCTCTTGGTGCTCAACTTTTGCATGTATGTCATTGTTCTAGGCATTGGTGCATGGGCTATGAATAGAGCAATAGATCATGGTTTTATCATAGGTCCGGAATTAAATCTTCCAGCTCATTTTTCGCCCATTTTCTTCCCAATGGGAAATGCTTCAACTGGATTCTTTGTTACGATTTCTTTGTTAGCTGGAGTAGTTGGTGCTGCATCGACAATTTCAGGAGTCAAACATATCTGTTCATGGACTGCAGAGAGCTTGCCATCTGCTGCTTCAATTGCTACTTTGGCTTGGACTCTTACACTGCTTGCCATGGGATTTGCATGGAAAGAGATTGAGCTTCAAATTAGAAATGCACGTCTGAAAACCATGGAGGCTTTCATAATTATCCTTTCAGTTACACAACTTCTCTACATAGTTGCAATTCATGGTGCTGCTGCATATAATATATATATTTGA

Protein sequence

>MsG0580027135.01.T01

MKSIATLLLVLNFCMYVIVLGIGAWAMNRAIDHGFIIGPELNLPAHFSPIFFPMGNASTGFFVTISLLAGVVGAASTISGVKHICSWTAESLPSAASIATLAWTLTLLAMGFAWKEIELQIRNARLKTMEAFIIILSVTQLLYIVAIHGAAAYNIYI*