AlfalfaGEDB | MsG0380013239.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013239.01

KEGG: -
GO: -
PFAM: PF16720

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013239.01.T01	XP_013459315.1	85.366	82	12	0	1	82	73	154	2.52E-44	150

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013239.01.T01	P62929	64.706	51	18	0	1	51	66	116	4.78E-19	78.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013239.01.T01	I3T793	85.366	82	12	0	1	82	73	154	1.20e-44	150

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048633.01	MsG0380013239.01	0.801209	1.003116e-48	4.762959e-46
MsG0080049129.01	MsG0380013239.01	-0.804903	1.727644e-49	9.016227e-47
MsG0180000367.01	MsG0380013239.01	0.800827	1.200798e-48	5.646536e-46
MsG0180001079.01	MsG0380013239.01	0.839711	1.345250e-57	1.873332e-54
MsG0180001503.01	MsG0380013239.01	0.803616	3.202141e-49	1.616533e-46
MsG0180002255.01	MsG0380013239.01	0.806691	7.273768e-50	3.973540e-47
MsG0180002935.01	MsG0380013239.01	0.805514	1.287038e-49	6.822152e-47
MsG0180003372.01	MsG0380013239.01	0.815082	1.109452e-51	7.574603e-49
MsG0180003375.01	MsG0380013239.01	0.819262	1.274003e-52	9.755708e-50
MsG0180003484.01	MsG0380013239.01	0.819053	1.421061e-52	1.081970e-49
MsG0180004424.01	MsG0380013239.01	0.838227	3.259849e-57	4.337743e-54
MsG0180005059.01	MsG0380013239.01	0.835641	1.490923e-56	1.834211e-53
MsG0180005060.01	MsG0380013239.01	0.816281	5.994895e-52	4.229947e-49
MsG0180005080.01	MsG0380013239.01	0.813912	2.012685e-51	1.331335e-48
MsG0180005082.01	MsG0380013239.01	0.805426	1.342470e-49	7.100196e-47
MsG0180005353.01	MsG0380013239.01	0.805715	1.167448e-49	6.220968e-47
MsG0280010623.01	MsG0380013239.01	0.807082	6.013874e-50	3.318674e-47
MsG0380012889.01	MsG0380013239.01	0.804167	2.460010e-49	1.259569e-46
MsG0380013212.01	MsG0380013239.01	0.928516	2.584906e-92	1.368500e-87
MsG0380013213.01	MsG0380013239.01	0.924071	1.149138e-89	4.759449e-85
MsG0380013214.01	MsG0380013239.01	0.920163	1.811632e-87	6.049101e-83
MsG0380013219.01	MsG0380013239.01	0.908724	1.250502e-81	2.352572e-77
MsG0380013226.01	MsG0380013239.01	0.916251	2.230355e-85	6.081080e-81
MsG0380013239.01	MsG0380014272.01	0.802303	5.981875e-49	2.920306e-46
MsG0380013239.01	MsG0380014410.01	0.814516	1.480266e-51	9.954051e-49
MsG0380013239.01	MsG0380016458.01	0.802676	5.011474e-49	2.469728e-46
MsG0380013239.01	MsG0380017200.01	0.803362	3.614637e-49	1.812785e-46
MsG0380013239.01	MsG0380017666.01	0.802470	5.526533e-49	2.709368e-46
MsG0380013239.01	MsG0380017918.01	-0.808029	3.785676e-50	2.141337e-47
MsG0380013239.01	MsG0480018406.01	0.809799	1.582822e-50	9.382878e-48
MsG0380013239.01	MsG0480018409.01	0.814619	1.404820e-51	9.472320e-49
MsG0380013239.01	MsG0480018439.01	0.808645	2.798091e-50	1.608460e-47
MsG0380013239.01	MsG0480018692.01	0.811421	7.062318e-51	4.369647e-48
MsG0380013239.01	MsG0480019346.01	0.800882	1.169929e-48	5.509475e-46
MsG0380013239.01	MsG0480019666.01	0.814807	1.276554e-51	8.651266e-49
MsG0380013239.01	MsG0480020814.01	0.817289	3.562700e-52	2.583892e-49
MsG0380013239.01	MsG0480021133.01	0.817280	3.578577e-52	2.594731e-49
MsG0380013239.01	MsG0480023322.01	0.821382	4.159287e-53	3.378469e-50
MsG0380013239.01	MsG0480023429.01	0.813827	2.101323e-51	1.386804e-48
MsG0380013239.01	MsG0580024567.01	-0.814316	1.638979e-51	1.096161e-48
MsG0380013239.01	MsG0580025007.01	0.828989	6.616357e-55	6.682109e-52
MsG0380013239.01	MsG0580025023.01	0.807992	3.855103e-50	2.178484e-47
MsG0380013239.01	MsG0580025024.01	0.833639	4.750784e-56	5.503830e-53
MsG0380013239.01	MsG0580025193.01	0.833360	5.578236e-56	6.409208e-53
MsG0380013239.01	MsG0580026148.01	0.812521	4.065093e-51	2.590452e-48
MsG0380013239.01	MsG0580027249.01	-0.800242	1.579626e-48	7.318636e-46
MsG0380013239.01	MsG0580027254.01	-0.804742	1.866337e-49	9.699518e-47
MsG0380013239.01	MsG0580029518.01	0.815305	9.896541e-52	6.798207e-49
MsG0380013239.01	MsG0580029991.01	-0.802826	4.665685e-49	2.308396e-46
MsG0380013239.01	MsG0680030379.01	0.819758	9.814347e-53	7.620582e-50
MsG0380013239.01	MsG0680032461.01	0.818580	1.820178e-52	1.367789e-49
MsG0380013239.01	MsG0680033467.01	0.806343	8.615323e-50	4.664709e-47
MsG0380013239.01	MsG0680034974.01	0.843552	1.306238e-58	2.051117e-55
MsG0380013239.01	MsG0680034978.01	0.814259	1.687154e-51	1.126610e-48
MsG0380013239.01	MsG0780036054.01	0.810243	1.270126e-50	7.616792e-48
MsG0380013239.01	MsG0780036259.01	0.805640	1.210685e-49	6.438584e-47
MsG0380013239.01	MsG0780036376.01	0.823465	1.364837e-53	1.176038e-50
MsG0380013239.01	MsG0780038307.01	0.869874	2.232224e-66	8.509275e-63
MsG0380013239.01	MsG0780038519.01	0.810610	1.058396e-50	6.409118e-48
MsG0380013239.01	MsG0780039883.01	0.803718	3.050232e-49	1.543828e-46
MsG0380013239.01	MsG0780039916.01	0.807985	3.868537e-50	2.185663e-47
MsG0380013239.01	MsG0780039923.01	0.820102	8.191713e-53	6.422369e-50
MsG0380013239.01	MsG0780040670.01	0.818290	2.117122e-52	1.578249e-49
MsG0380013239.01	MsG0780041036.01	0.806998	6.264115e-50	3.449287e-47
MsG0380013239.01	MsG0780041447.01	0.818695	1.714175e-52	1.292201e-49
MsG0380013239.01	MsG0880042122.01	0.802830	4.657195e-49	2.304397e-46
MsG0380013239.01	MsG0880042633.01	0.834459	2.961837e-56	3.517331e-53
MsG0380013239.01	MsG0880042642.01	0.834820	2.402574e-56	2.883908e-53
MsG0380013239.01	MsG0880043530.01	0.805587	1.242043e-49	6.596316e-47
MsG0380013239.01	MsG0880044312.01	-0.805187	1.506481e-49	7.918885e-47
MsG0380013239.01	MsG0880045854.01	0.823716	1.192396e-53	1.034952e-50
MsG0380013239.01	MsG0880046166.01	0.850029	2.211220e-60	4.273945e-57
MsG0380013239.01	MsG0880046402.01	0.807832	4.169183e-50	2.346067e-47
MsG0380013239.01	MsG0880046403.01	0.824134	9.514781e-54	8.354992e-51
MsG0380013239.01	MsG0880046838.01	-0.805721	1.164449e-49	6.205878e-47
MsG0380013239.01	MsG0880047697.01	0.847040	1.487225e-59	2.609905e-56
MsG0380013239.01	MsG0880047714.01	0.806059	9.886501e-50	5.314668e-47
MsG0280007670.01	MsG0380013239.01	0.827456	1.548787e-54	1.496078e-51
MsG0280007678.01	MsG0380013239.01	0.803026	4.241987e-49	2.109225e-46
MsG0280007689.01	MsG0380013239.01	0.821209	4.560689e-53	3.686970e-50
MsG0280008226.01	MsG0380013239.01	0.838064	3.590087e-57	4.753913e-54
MsG0280009253.01	MsG0380013239.01	0.805877	1.079546e-49	5.776194e-47
MsG0280010425.01	MsG0380013239.01	0.855848	4.789850e-62	1.121032e-58

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013239.01.T01	MTR_3g436100	85.366	82	12	0	1	82	73	154	3.05e-48	150
MsG0380013239.01.T01	MTR_7g044920	62.651	83	30	1	1	82	70	152	1.69e-34	115
MsG0380013239.01.T01	MTR_7g044980	62.195	82	30	1	2	82	64	145	4.91e-33	111
MsG0380013239.01.T01	MTR_8g056800	53.086	81	38	0	2	82	21	101	1.16e-27	96.3
MsG0380013239.01.T01	MTR_6g017170	71.429	56	15	1	1	55	68	123	1.06e-24	89.7
MsG0380013239.01.T01	MTR_8g022400	54.795	73	32	1	2	73	69	141	3.28e-24	89.0
MsG0380013239.01.T01	MTR_3g067437	68.519	54	17	0	2	55	69	122	1.29e-23	87.0
MsG0380013239.01.T01	MTR_8g022420	62.500	64	23	1	2	64	68	131	3.07e-23	86.3
MsG0380013239.01.T01	MTR_8g022430	59.091	66	25	2	1	64	68	133	1.84e-22	84.3
MsG0380013239.01.T01	MTR_6g017150	55.844	77	22	2	1	76	68	133	4.43e-22	83.6
MsG0380013239.01.T01	MTR_3g067500	54.839	62	28	0	1	62	46	107	2.31e-21	80.9
MsG0380013239.01.T01	MTR_3g067540	50.649	77	27	2	2	78	73	138	9.82e-21	80.1
MsG0380013239.01.T01	MTR_3g067555	50.649	77	27	2	2	78	73	138	9.82e-21	80.1
MsG0380013239.01.T01	MTR_3g067555	50.649	77	27	2	2	78	60	125	1.00e-20	79.7
MsG0380013239.01.T01	MTR_3g067550	50.649	77	27	2	2	78	52	117	1.10e-20	79.3
MsG0380013239.01.T01	MTR_3g067555	50.649	77	27	2	2	78	52	117	1.10e-20	79.3
MsG0380013239.01.T01	MTR_3g467760	52.941	68	31	1	2	68	43	110	1.76e-20	78.6
MsG0380013239.01.T01	MTR_3g067430	48.052	77	29	1	2	78	73	138	1.88e-20	79.3
MsG0380013239.01.T01	MTR_7g056803	57.377	61	25	1	5	64	74	134	2.33e-20	79.0
MsG0380013239.01.T01	MTR_7g056817	53.846	65	29	1	1	64	71	135	2.49e-20	79.0
MsG0380013239.01.T01	MTR_3g067580	54.098	61	28	0	2	62	77	137	4.02e-20	78.6
MsG0380013239.01.T01	MTR_3g067270	52.941	68	31	1	2	68	67	134	4.95e-20	78.2
MsG0380013239.01.T01	MTR_3g467750	52.941	68	31	1	2	68	67	134	4.95e-20	78.2
MsG0380013239.01.T01	MTR_3g067280	52.941	68	31	1	2	68	67	134	5.58e-20	77.8
MsG0380013239.01.T01	MTR_3g463570	47.500	80	30	2	2	80	68	136	6.93e-20	77.8
MsG0380013239.01.T01	MTR_3g067570	67.391	46	15	0	2	47	45	90	1.42e-19	75.9
MsG0380013239.01.T01	MTR_3g067535	53.247	77	23	2	2	78	71	134	1.80e-19	76.6
MsG0380013239.01.T01	MTR_3g463570	47.500	80	30	2	2	80	88	156	2.27e-19	77.0
MsG0380013239.01.T01	MTR_6g036620	50.000	78	28	2	1	78	66	132	6.52e-19	75.1
MsG0380013239.01.T01	MTR_4g026590	47.561	82	32	2	1	82	75	145	7.36e-19	75.5
MsG0380013239.01.T01	MTR_3g067510	67.391	46	15	0	2	47	75	120	1.12e-18	74.7
MsG0380013239.01.T01	MTR_6g047900	46.341	82	31	3	1	81	65	134	1.29e-18	74.3
MsG0380013239.01.T01	MTR_3g067510	67.391	46	15	0	2	47	70	115	1.41e-18	74.3
MsG0380013239.01.T01	MTR_8g461020	47.945	73	27	2	1	72	67	129	5.26e-18	72.8
MsG0380013239.01.T01	MTR_6g047880	44.444	81	33	2	1	81	69	137	1.91e-17	71.6
MsG0380013239.01.T01	MTR_1g019650	50.980	51	25	0	5	55	60	110	2.42e-15	65.5
MsG0380013239.01.T01	MTR_3g067445	55.556	45	20	0	2	46	73	117	2.53e-15	65.5
MsG0380013239.01.T01	MTR_3g436120	80.000	40	8	0	43	82	62	101	7.32e-15	63.9
MsG0380013239.01.T01	MTR_7g029540	40.385	52	31	0	1	52	69	120	1.23e-11	56.2
MsG0380013239.01.T01	MTR_6g082060	59.524	42	17	0	19	60	91	132	1.77e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 14 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTAATGCTGATATTGAATA+TGG	0.285356	3:+33606000	MsG0380013239.01.T01:CDS
GCAGAACGTTATTTCAAGAT+TGG	0.331624	3:+33606048	MsG0380013239.01.T01:CDS
AGACTACTTGAAGATGGCTT+TGG	0.386657	3:+33606125	MsG0380013239.01.T01:CDS
CGAGACATATCATTCTTAAG+TGG	0.433054	3:-33606154	None:intergenic
CCATATTCAATATCAGCATT+AGG	0.437256	3:-33606000	None:intergenic
GAGTGCATCAAGAAGGGAAG+TGG	0.522512	3:+33605958	MsG0380013239.01.T01:CDS
TCATGCCGAGTGCATCAAGA+AGG	0.559241	3:+33605951	MsG0380013239.01.T01:CDS
ACTTCCCTTCTTGATGCACT+CGG	0.605609	3:-33605956	None:intergenic
CGTTCTGCTTCATCTACAGA+GGG	0.611592	3:-33606033	None:intergenic
CATGCCGAGTGCATCAAGAA+GGG	0.625240	3:+33605952	MsG0380013239.01.T01:CDS
ATGCTGATATTGAATATGGA+TGG	0.639133	3:+33606004	MsG0380013239.01.T01:CDS
AGTGCATCAAGAAGGGAAGT+GGG	0.648471	3:+33605959	MsG0380013239.01.T01:CDS
ACGTTCTGCTTCATCTACAG+AGG	0.649348	3:-33606034	None:intergenic
ACATAATATGAAGATGATCG+AGG	0.692790	3:+33605906	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGAAGAATTTTTTTACTACA+GGG	-	Chr3:33606080-33606099	None:intergenic	20.0%
!!!	TTGAAGAATTTTTTTACTAC+AGG	-	Chr3:33606081-33606100	None:intergenic	20.0%
	ATGCTGATATTGAATATGGA+TGG	+	Chr3:33606004-33606023	MsG0380013239.01.T01:CDS	30.0%
	CCATATTCAATATCAGCATT+AGG	-	Chr3:33606003-33606022	None:intergenic	30.0%
	CCTAATGCTGATATTGAATA+TGG	+	Chr3:33606000-33606019	MsG0380013239.01.T01:CDS	30.0%
	TGAAAAAGACTACTTGAAGA+TGG	+	Chr3:33606119-33606138	MsG0380013239.01.T01:CDS	30.0%
!!	GTCTTTTTCATTAGAGTTTG+AGG	-	Chr3:33606109-33606128	None:intergenic	30.0%
!!!	GAATTTTTTTACTACAGGGT+TGG	-	Chr3:33606076-33606095	None:intergenic	30.0%
!	GCAGAACGTTATTTCAAGAT+TGG	+	Chr3:33606048-33606067	MsG0380013239.01.T01:CDS	35.0%
!	GCATGAGTTTTGCATAAGTT+AGG	-	Chr3:33605937-33605956	None:intergenic	35.0%
	AGACTACTTGAAGATGGCTT+TGG	+	Chr3:33606125-33606144	MsG0380013239.01.T01:CDS	40.0%
	ACGTTCTGCTTCATCTACAG+AGG	-	Chr3:33606037-33606056	None:intergenic	45.0%
	ACTTCCCTTCTTGATGCACT+CGG	-	Chr3:33605959-33605978	None:intergenic	45.0%
	AGTGCATCAAGAAGGGAAGT+GGG	+	Chr3:33605959-33605978	MsG0380013239.01.T01:CDS	45.0%
	CGTTCTGCTTCATCTACAGA+GGG	-	Chr3:33606036-33606055	None:intergenic	45.0%
	CATGCCGAGTGCATCAAGAA+GGG	+	Chr3:33605952-33605971	MsG0380013239.01.T01:CDS	50.0%
	GAGTGCATCAAGAAGGGAAG+TGG	+	Chr3:33605958-33605977	MsG0380013239.01.T01:CDS	50.0%
	TCATGCCGAGTGCATCAAGA+AGG	+	Chr3:33605951-33605970	MsG0380013239.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	33605913	33606161	33605913	ID=MsG0380013239.01;Name=MsG0380013239.01
Chr3	mRNA	33605913	33606161	33605913	ID=MsG0380013239.01.T01;Parent=MsG0380013239.01;Name=MsG0380013239.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|82
Chr3	exon	33605913	33606161	33605913	ID=MsG0380013239.01.T01:exon:5335;Parent=MsG0380013239.01.T01
Chr3	CDS	33605913	33606161	33605913	ID=MsG0380013239.01.T01:cds;Parent=MsG0380013239.01.T01

Gene Sequence	>MsG0380013239.01.T01 ATGAAGATGATCGAGGAACATCCTAACTTATGCAAAACTCATGCCGAGTGCATCAAGAAGGGAAGTGGGAACTTTTGTGCTCGTTATCCTAATGCTGATATTGAATATGGATGGTGTTTTCCCTCTGTAGATGAAGCAGAACGTTATTTCAAGATTGGATCCAACCCTGTAGTAAAAAAATTCTTCAAGATTGCCTCAAACTCTAATGAAAAAGACTACTTGAAGATGGCTTTGGAAATTGCCACTTAA
Protein sequence	>MsG0380013239.01.T01 MKMIEEHPNLCKTHAECIKKGSGNFCARYPNADIEYGWCFPSVDEAERYFKIGSNPVVKKFFKIASNSNEKDYLKMALEIAT*