Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006579.01.T01 | XP_004485679.1 | 87.685 | 203 | 25 | 0 | 45 | 247 | 1 | 203 | 3.65E-126 | 366 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006579.01.T01 | Q6NQE2 | 81.095 | 201 | 38 | 0 | 45 | 245 | 1 | 201 | 4.36E-119 | 341 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006579.01.T01 | A0A1S2XC58 | 87.685 | 203 | 25 | 0 | 45 | 247 | 1 | 203 | 1.75e-126 | 366 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047985.01 | MsG0280006579.01 | 0.812825 | 3.488785e-51 | 2.240772e-48 |
| MsG0080048134.01 | MsG0280006579.01 | 0.806538 | 7.836863e-50 | 4.264509e-47 |
| MsG0180000367.01 | MsG0280006579.01 | 0.807639 | 4.581178e-50 | 2.564956e-47 |
| MsG0180000466.01 | MsG0280006579.01 | 0.819382 | 1.195909e-52 | 9.187916e-50 |
| MsG0180001029.01 | MsG0280006579.01 | 0.825181 | 5.392760e-54 | 4.879906e-51 |
| MsG0180001503.01 | MsG0280006579.01 | 0.813366 | 2.654106e-51 | 1.729930e-48 |
| MsG0180001515.01 | MsG0280006579.01 | 0.815249 | 1.018562e-51 | 6.985602e-49 |
| MsG0180003320.01 | MsG0280006579.01 | 0.839441 | 1.581968e-57 | 2.184933e-54 |
| MsG0180003322.01 | MsG0280006579.01 | 0.814645 | 1.386597e-51 | 9.356205e-49 |
| MsG0180003375.01 | MsG0280006579.01 | 0.817649 | 2.955924e-52 | 2.165087e-49 |
| MsG0180003405.01 | MsG0280006579.01 | -0.814258 | 1.688190e-51 | 1.127261e-48 |
| MsG0180003484.01 | MsG0280006579.01 | 0.843236 | 1.586004e-58 | 2.465583e-55 |
| MsG0180003505.01 | MsG0280006579.01 | 0.803924 | 2.763468e-49 | 1.406258e-46 |
| MsG0180004121.01 | MsG0280006579.01 | 0.801042 | 1.085170e-48 | 5.131201e-46 |
| MsG0180004549.01 | MsG0280006579.01 | 0.848921 | 4.504164e-60 | 8.397127e-57 |
| MsG0180004948.01 | MsG0280006579.01 | 0.826907 | 2.096375e-54 | 1.992801e-51 |
| MsG0180005127.01 | MsG0280006579.01 | 0.815654 | 8.274982e-52 | 5.739383e-49 |
| MsG0180005146.01 | MsG0280006579.01 | 0.820732 | 5.871418e-53 | 4.683611e-50 |
| MsG0180005267.01 | MsG0280006579.01 | 0.832079 | 1.159999e-55 | 1.282875e-52 |
| MsG0180005571.01 | MsG0280006579.01 | 0.810468 | 1.135694e-50 | 6.851235e-48 |
| MsG0180005906.01 | MsG0280006579.01 | 0.852367 | 4.836246e-61 | 1.008294e-57 |
| MsG0280006546.01 | MsG0280006579.01 | 0.803415 | 3.523819e-49 | 1.769775e-46 |
| MsG0280006575.01 | MsG0280006579.01 | 0.821759 | 3.403214e-53 | 2.793796e-50 |
| MsG0280006579.01 | MsG0280007199.01 | 0.822730 | 2.025652e-53 | 1.709707e-50 |
| MsG0280006579.01 | MsG0280007201.01 | 0.822917 | 1.832636e-53 | 1.554972e-50 |
| MsG0280006579.01 | MsG0280009167.01 | 0.802869 | 4.571016e-49 | 2.263862e-46 |
| MsG0280006579.01 | MsG0280009232.01 | 0.809602 | 1.744633e-50 | 1.028864e-47 |
| MsG0280006579.01 | MsG0280009526.01 | 0.840236 | 9.821422e-58 | 1.390291e-54 |
| MsG0280006579.01 | MsG0280009657.01 | 0.826721 | 2.322179e-54 | 2.195315e-51 |
| MsG0280006579.01 | MsG0280010425.01 | 0.812349 | 4.434312e-51 | 2.812758e-48 |
| MsG0280006579.01 | MsG0280010936.01 | 0.819822 | 9.491618e-53 | 7.382825e-50 |
| MsG0280006579.01 | MsG0280011128.01 | 0.813285 | 2.765601e-51 | 1.798493e-48 |
| MsG0280006579.01 | MsG0280011269.01 | 0.815315 | 9.844898e-52 | 6.764714e-49 |
| MsG0280006579.01 | MsG0280011281.01 | -0.809987 | 1.442181e-50 | 8.591470e-48 |
| MsG0280006579.01 | MsG0380011968.01 | 0.800508 | 1.394444e-48 | 6.504350e-46 |
| MsG0280006579.01 | MsG0380011974.01 | 0.807028 | 6.172820e-50 | 3.401755e-47 |
| MsG0280006579.01 | MsG0380012222.01 | 0.807631 | 4.600509e-50 | 2.575274e-47 |
| MsG0280006579.01 | MsG0380012889.01 | 0.807421 | 5.096153e-50 | 2.837277e-47 |
| MsG0280006579.01 | MsG0380012900.01 | 0.807580 | 4.715747e-50 | 2.636149e-47 |
| MsG0280006579.01 | MsG0380013226.01 | 0.812578 | 3.951286e-51 | 2.521589e-48 |
| MsG0280006579.01 | MsG0380013843.01 | 0.859048 | 5.413073e-63 | 1.410383e-59 |
| MsG0280006579.01 | MsG0380014642.01 | -0.804179 | 2.445874e-49 | 1.252722e-46 |
| MsG0280006579.01 | MsG0380015711.01 | 0.820487 | 6.684385e-53 | 5.296119e-50 |
| MsG0280006579.01 | MsG0380016299.01 | 0.819883 | 9.191404e-53 | 7.161552e-50 |
| MsG0280006579.01 | MsG0380016300.01 | 0.816442 | 5.519116e-52 | 3.910916e-49 |
| MsG0280006579.01 | MsG0380016490.01 | 0.851569 | 8.149448e-61 | 1.656422e-57 |
| MsG0280006579.01 | MsG0380017030.01 | 0.804512 | 2.085092e-49 | 1.077074e-46 |
| MsG0280006579.01 | MsG0380017168.01 | 0.810401 | 1.174323e-50 | 7.071327e-48 |
| MsG0280006579.01 | MsG0380017594.01 | 0.812299 | 4.546113e-51 | 2.879752e-48 |
| MsG0280006579.01 | MsG0380017901.01 | 0.849341 | 3.441075e-60 | 6.503652e-57 |
| MsG0280006579.01 | MsG0480019146.01 | 0.834799 | 2.431465e-56 | 2.916589e-53 |
| MsG0280006579.01 | MsG0480019171.01 | 0.841777 | 3.868270e-58 | 5.743933e-55 |
| MsG0280006579.01 | MsG0480019663.01 | 0.808143 | 3.578983e-50 | 2.030663e-47 |
| MsG0280006579.01 | MsG0480019842.01 | 0.804847 | 1.774701e-49 | 9.247959e-47 |
| MsG0280006579.01 | MsG0480020810.01 | 0.848718 | 5.127265e-60 | 9.495819e-57 |
| MsG0280006579.01 | MsG0480020904.01 | 0.810311 | 1.227457e-50 | 7.374090e-48 |
| MsG0280006579.01 | MsG0480021133.01 | 0.809615 | 1.733192e-50 | 1.022517e-47 |
| MsG0280006579.01 | MsG0480021230.01 | 0.800137 | 1.659259e-48 | 7.667286e-46 |
| MsG0280006579.01 | MsG0480021360.01 | 0.800683 | 1.284722e-48 | 6.019198e-46 |
| MsG0280006579.01 | MsG0480021967.01 | -0.811586 | 6.502751e-51 | 4.040845e-48 |
| MsG0280006579.01 | MsG0480022055.01 | 0.843080 | 1.746149e-58 | 2.700972e-55 |
| MsG0280006579.01 | MsG0480022428.01 | 0.806390 | 8.421477e-50 | 4.565206e-47 |
| MsG0280006579.01 | MsG0480022886.01 | 0.807441 | 5.048384e-50 | 2.812054e-47 |
| MsG0280006579.01 | MsG0480023067.01 | 0.800301 | 1.536539e-48 | 7.130099e-46 |
| MsG0280006579.01 | MsG0480023322.01 | 0.802239 | 6.164973e-49 | 3.004657e-46 |
| MsG0280006579.01 | MsG0480023432.01 | 0.816603 | 5.077810e-52 | 3.614379e-49 |
| MsG0280006579.01 | MsG0480023851.01 | -0.814194 | 1.744164e-51 | 1.162591e-48 |
| MsG0280006579.01 | MsG0580024087.01 | 0.814047 | 1.879989e-51 | 1.248124e-48 |
| MsG0280006579.01 | MsG0580024980.01 | 0.825398 | 4.791204e-54 | 4.362087e-51 |
| MsG0280006579.01 | MsG0580025007.01 | 0.804376 | 2.225769e-49 | 1.145792e-46 |
| MsG0280006579.01 | MsG0580025024.01 | 0.809708 | 1.655738e-50 | 9.791533e-48 |
| MsG0280006579.01 | MsG0580025432.01 | 0.831463 | 1.646070e-55 | 1.787635e-52 |
| MsG0280006579.01 | MsG0580025550.01 | 0.809705 | 1.657805e-50 | 9.803051e-48 |
| MsG0280006579.01 | MsG0580025633.01 | 0.829241 | 5.747055e-55 | 5.848592e-52 |
| MsG0280006579.01 | MsG0580026180.01 | 0.835253 | 1.868214e-56 | 2.272142e-53 |
| MsG0280006579.01 | MsG0580026521.01 | 0.829228 | 5.788783e-55 | 5.888766e-52 |
| MsG0280006579.01 | MsG0580027276.01 | -0.805816 | 1.111822e-49 | 5.939750e-47 |
| MsG0280006579.01 | MsG0580028302.01 | 0.820013 | 8.584826e-53 | 6.713455e-50 |
| MsG0280006579.01 | MsG0580029676.01 | 0.800783 | 1.225696e-48 | 5.757156e-46 |
| MsG0280006579.01 | MsG0580029938.01 | 0.817335 | 3.477583e-52 | 2.525473e-49 |
| MsG0280006579.01 | MsG0680030296.01 | 0.845003 | 5.322979e-59 | 8.748235e-56 |
| MsG0280006579.01 | MsG0680030613.01 | 0.805537 | 1.272453e-49 | 6.748940e-47 |
| MsG0280006579.01 | MsG0680031200.01 | 0.833244 | 5.962498e-56 | 6.826679e-53 |
| MsG0280006579.01 | MsG0680034238.01 | 0.833557 | 4.980412e-56 | 5.756153e-53 |
| MsG0280006579.01 | MsG0680034354.01 | 0.800517 | 1.388812e-48 | 6.479622e-46 |
| MsG0280006579.01 | MsG0680034418.01 | 0.813823 | 2.105455e-51 | 1.389395e-48 |
| MsG0280006579.01 | MsG0680034728.01 | 0.803194 | 3.916703e-49 | 1.955958e-46 |
| MsG0280006579.01 | MsG0680034978.01 | 0.833671 | 4.664672e-56 | 5.409258e-53 |
| MsG0280006579.01 | MsG0780036054.01 | 0.815442 | 9.223622e-52 | 6.360189e-49 |
| MsG0280006579.01 | MsG0780036649.01 | 0.802032 | 6.801532e-49 | 3.297351e-46 |
| MsG0280006579.01 | MsG0780036839.01 | 0.825794 | 3.860751e-54 | 3.555085e-51 |
| MsG0280006579.01 | MsG0780038305.01 | 0.813320 | 2.717190e-51 | 1.768772e-48 |
| MsG0280006579.01 | MsG0780038307.01 | 0.816017 | 6.869553e-52 | 4.812072e-49 |
| MsG0280006579.01 | MsG0780038914.01 | 0.824831 | 6.520731e-54 | 5.841990e-51 |
| MsG0280006579.01 | MsG0780038919.01 | 0.819720 | 1.001605e-52 | 7.768620e-50 |
| MsG0280006579.01 | MsG0780039619.01 | 0.822607 | 2.164050e-53 | 1.820275e-50 |
| MsG0280006579.01 | MsG0780039855.01 | 0.816807 | 4.570172e-52 | 3.271178e-49 |
| MsG0280006579.01 | MsG0780039883.01 | 0.842834 | 2.030021e-58 | 3.116056e-55 |
| MsG0280006579.01 | MsG0780039910.01 | 0.810195 | 1.300840e-50 | 7.790833e-48 |
| MsG0280006579.01 | MsG0780039912.01 | 0.803555 | 3.296072e-49 | 1.661468e-46 |
| MsG0280006579.01 | MsG0780039979.01 | 0.804437 | 2.160769e-49 | 1.114052e-46 |
| MsG0280006579.01 | MsG0780040497.01 | 0.827027 | 1.962540e-54 | 1.871984e-51 |
| MsG0280006579.01 | MsG0780040498.01 | 0.847129 | 1.406005e-59 | 2.474719e-56 |
| MsG0280006579.01 | MsG0780040560.01 | 0.807082 | 6.013972e-50 | 3.318722e-47 |
| MsG0280006579.01 | MsG0780040716.01 | 0.837650 | 4.586935e-57 | 5.998070e-54 |
| MsG0280006579.01 | MsG0780040719.01 | 0.804010 | 2.652227e-49 | 1.352566e-46 |
| MsG0280006579.01 | MsG0780040847.01 | 0.802385 | 5.754101e-49 | 2.814989e-46 |
| MsG0280006579.01 | MsG0780041248.01 | 0.806710 | 7.208535e-50 | 3.939826e-47 |
| MsG0280006579.01 | MsG0780041397.01 | 0.815490 | 9.002408e-52 | 6.215972e-49 |
| MsG0280006579.01 | MsG0780041403.01 | 0.815350 | 9.672996e-52 | 6.652849e-49 |
| MsG0280006579.01 | MsG0780041500.01 | 0.805343 | 1.397625e-49 | 7.376030e-47 |
| MsG0280006579.01 | MsG0880042122.01 | 0.817009 | 4.118938e-52 | 2.964693e-49 |
| MsG0280006579.01 | MsG0880042261.01 | 0.805685 | 1.184667e-49 | 6.307775e-47 |
| MsG0280006579.01 | MsG0880043212.01 | 0.803420 | 3.516460e-49 | 1.766282e-46 |
| MsG0280006579.01 | MsG0880043495.01 | 0.800445 | 1.436547e-48 | 6.690129e-46 |
| MsG0280006579.01 | MsG0880043550.01 | 0.816814 | 4.554852e-52 | 3.260844e-49 |
| MsG0280006579.01 | MsG0880043882.01 | 0.826294 | 2.936013e-54 | 2.742130e-51 |
| MsG0280006579.01 | MsG0880043906.01 | 0.805288 | 1.434920e-49 | 7.562339e-47 |
| MsG0280006579.01 | MsG0880044712.01 | 0.820364 | 7.133685e-53 | 5.632863e-50 |
| MsG0280006579.01 | MsG0880045130.01 | 0.815265 | 1.010105e-51 | 6.931090e-49 |
| MsG0280006579.01 | MsG0880045801.01 | 0.810121 | 1.349452e-50 | 8.067022e-48 |
| MsG0280006579.01 | MsG0880045854.01 | 0.843289 | 1.535594e-58 | 2.391487e-55 |
| MsG0280006579.01 | MsG0880046010.01 | -0.820725 | 5.893572e-53 | 4.700373e-50 |
| MsG0280006579.01 | MsG0880046402.01 | 0.800151 | 1.648889e-48 | 7.621824e-46 |
| MsG0280006579.01 | MsG0880046870.01 | 0.833317 | 5.717346e-56 | 6.560791e-53 |
| MsG0280006579.01 | MsG0880047383.01 | 0.826521 | 2.592624e-54 | 2.437039e-51 |
| MsG0280006579.01 | MsG0880047664.01 | 0.821936 | 3.096795e-53 | 2.555077e-50 |
| MsG0280006579.01 | MsG0880047714.01 | 0.820653 | 6.120687e-53 | 4.871580e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006579.01.T01 | MTR_8g466030 | 83.000 | 200 | 34 | 0 | 45 | 244 | 1 | 200 | 2.01e-122 | 347 |
| MsG0280006579.01.T01 | MTR_4g091900 | 82.587 | 201 | 35 | 0 | 47 | 247 | 4 | 204 | 2.13e-121 | 344 |
| MsG0280006579.01.T01 | MTR_2g081810 | 82.000 | 200 | 36 | 0 | 45 | 244 | 1 | 200 | 5.50e-120 | 340 |
| MsG0280006579.01.T01 | MTR_4g091900 | 81.771 | 192 | 35 | 0 | 56 | 247 | 1 | 192 | 4.81e-114 | 325 |
| MsG0280006579.01.T01 | MTR_2g011080 | 75.369 | 203 | 50 | 0 | 45 | 247 | 1 | 203 | 4.26e-110 | 315 |
| MsG0280006579.01.T01 | MTR_2g011080 | 74.359 | 195 | 50 | 0 | 53 | 247 | 4 | 198 | 4.10e-104 | 300 |
| MsG0280006579.01.T01 | MTR_5g070030 | 67.839 | 199 | 63 | 1 | 47 | 244 | 4 | 202 | 7.75e-98 | 284 |
| MsG0280006579.01.T01 | MTR_4g069250 | 67.513 | 197 | 63 | 1 | 48 | 243 | 52 | 248 | 8.60e-92 | 271 |
| MsG0280006579.01.T01 | MTR_5g019620 | 64.500 | 200 | 70 | 1 | 48 | 246 | 53 | 252 | 3.97e-89 | 264 |
| MsG0280006579.01.T01 | MTR_2g011080 | 73.684 | 152 | 40 | 0 | 96 | 247 | 1 | 152 | 6.20e-79 | 234 |
| MsG0280006579.01.T01 | MTR_4g091890 | 83.333 | 120 | 16 | 1 | 128 | 247 | 1 | 116 | 8.18e-67 | 202 |
| MsG0280006579.01.T01 | MTR_8g466080 | 76.724 | 116 | 27 | 0 | 129 | 244 | 1 | 116 | 7.39e-60 | 185 |
| MsG0280006579.01.T01 | MTR_8g466110 | 54.032 | 124 | 31 | 2 | 96 | 219 | 1 | 98 | 2.35e-33 | 117 |
| MsG0280006579.01.T01 | MTR_8g466150 | 54.032 | 124 | 31 | 2 | 96 | 219 | 1 | 98 | 2.35e-33 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006579.01.T01 | AT4G27270 | 81.095 | 201 | 38 | 0 | 45 | 245 | 1 | 201 | 4.45e-120 | 341 |
| MsG0280006579.01.T01 | AT5G54500 | 80.693 | 202 | 39 | 0 | 45 | 246 | 1 | 202 | 2.71e-119 | 339 |
| MsG0280006579.01.T01 | AT4G27270 | 80.000 | 190 | 38 | 0 | 56 | 245 | 1 | 190 | 2.49e-111 | 318 |
| MsG0280006579.01.T01 | AT4G27270 | 80.000 | 190 | 38 | 0 | 56 | 245 | 1 | 190 | 2.49e-111 | 318 |
| MsG0280006579.01.T01 | AT5G58800 | 66.332 | 199 | 66 | 1 | 47 | 244 | 4 | 202 | 9.66e-95 | 276 |
| MsG0280006579.01.T01 | AT5G58800 | 66.332 | 199 | 66 | 1 | 47 | 244 | 4 | 202 | 9.66e-95 | 276 |
| MsG0280006579.01.T01 | AT5G54500 | 79.012 | 162 | 34 | 0 | 45 | 206 | 1 | 162 | 3.63e-91 | 269 |
| MsG0280006579.01.T01 | AT4G36750 | 64.975 | 197 | 68 | 1 | 48 | 243 | 74 | 270 | 5.48e-90 | 267 |
| MsG0280006579.01.T01 | AT4G27270 | 83.333 | 150 | 25 | 0 | 96 | 245 | 1 | 150 | 1.01e-89 | 262 |
Find 56 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTCATTTGCTGAAATAAT+TGG | 0.177953 | 2:+3653588 | None:intergenic |
| ACCTCTTCTGGCAATATTTC+AGG | 0.186131 | 2:+3653648 | None:intergenic |
| TACTCAATTCAAAGCATTTA+TGG | 0.233806 | 2:-3653511 | MsG0280006579.01.T01:CDS |
| AGAAACCAAAAGGGCGTTCT+TGG | 0.308627 | 2:+3654061 | None:intergenic |
| ATAATTGGAACATCACTCTT+TGG | 0.328363 | 2:+3653603 | None:intergenic |
| GTGCATTATATAAATGGAAT+TGG | 0.330285 | 2:-3654133 | None:intergenic |
| ACTCACCAAGAACGCCCTTT+TGG | 0.367633 | 2:-3654066 | MsG0280006579.01.T01:CDS |
| TTTAGCTACTATTCTATGTA+TGG | 0.386282 | 2:-3653862 | MsG0280006579.01.T01:intron |
| CATCAAGGGAAATACTTTGC+GGG | 0.388451 | 2:-3652670 | MsG0280006579.01.T01:CDS |
| TGGTGCACACAGAAACTTGC+AGG | 0.402973 | 2:-3653471 | MsG0280006579.01.T01:CDS |
| ATCTTCCCAAGAACCTCTTC+TGG | 0.413268 | 2:+3653636 | None:intergenic |
| CAAGTTTCTGTGTGCACCAT+AGG | 0.415808 | 2:+3653475 | None:intergenic |
| GCCATCATTCCAAATCTTGT+TGG | 0.419935 | 2:+3653534 | None:intergenic |
| AAAGCATTTATGGATACAAC+TGG | 0.420143 | 2:-3653501 | MsG0280006579.01.T01:CDS |
| TCCAACAAGATTTGGAATGA+TGG | 0.421484 | 2:-3653535 | MsG0280006579.01.T01:CDS |
| TATTGCCAGAAGAGGTTCTT+GGG | 0.434381 | 2:-3653641 | MsG0280006579.01.T01:CDS |
| GTGCCCGTTGGATACACATT+TGG | 0.442396 | 2:-3652805 | MsG0280006579.01.T01:CDS |
| CTGTCTCTATAGAAACCAAA+AGG | 0.458141 | 2:+3654051 | None:intergenic |
| TATTCTATGTATGGACATGT+TGG | 0.462480 | 2:-3653853 | MsG0280006579.01.T01:CDS |
| AATGGAGTTGAATCCATAGA+AGG | 0.462725 | 2:-3653808 | MsG0280006579.01.T01:CDS |
| TATGGTGCTGGAACTTATGC+AGG | 0.483004 | 2:-3652739 | MsG0280006579.01.T01:CDS |
| ATATTGCCAGAAGAGGTTCT+TGG | 0.489085 | 2:-3653642 | MsG0280006579.01.T01:CDS |
| GCATAAGTTCCAGCACCATA+TGG | 0.494566 | 2:+3652742 | None:intergenic |
| TCATCAAGGGAAATACTTTG+CGG | 0.497889 | 2:-3652671 | MsG0280006579.01.T01:CDS |
| ACAGCCCACTGGATTAGAAC+TGG | 0.499934 | 2:-3652704 | MsG0280006579.01.T01:CDS |
| AATGAGCTTCTTGAGGCTGA+TGG | 0.504019 | 2:-3653573 | MsG0280006579.01.T01:CDS |
| GTGAAAGGTGGCTCACCATA+TGG | 0.504157 | 2:-3652757 | MsG0280006579.01.T01:CDS |
| GCACCAAATGTGTATCCAAC+GGG | 0.505966 | 2:+3652802 | None:intergenic |
| GTTGGATACACATTTGGTGC+TGG | 0.509879 | 2:-3652799 | MsG0280006579.01.T01:CDS |
| ATTGGAACATCACTCTTTGG+AGG | 0.524679 | 2:+3653606 | None:intergenic |
| ATTCTATGTATGGACATGTT+GGG | 0.527530 | 2:-3653852 | MsG0280006579.01.T01:CDS |
| GTGCTGTAAAAGATGCCAGC+AGG | 0.530472 | 2:+3653444 | None:intergenic |
| CTTCAAGTTACTAGCAGCAA+TGG | 0.533135 | 2:-3654007 | MsG0280006579.01.T01:CDS |
| GCTGGAACTTATGCAGGGGA+TGG | 0.539914 | 2:-3652733 | MsG0280006579.01.T01:CDS |
| GGTGGCTCACCATATGGTGC+TGG | 0.541107 | 2:-3652751 | MsG0280006579.01.T01:CDS |
| GAATTGCAAAGAAGCTCAAA+GGG | 0.547992 | 2:-3652648 | MsG0280006579.01.T01:CDS |
| AAACTTGCAGGAAAGCCTGC+TGG | 0.549934 | 2:-3653459 | MsG0280006579.01.T01:CDS |
| TGTAGAAGCAAAACTCTGGC+AGG | 0.555434 | 2:-3653785 | MsG0280006579.01.T01:intron |
| TTCAGCAAATGAGCTTCTTG+AGG | 0.559049 | 2:-3653580 | MsG0280006579.01.T01:CDS |
| TGGATACAACTGGAGACCTA+TGG | 0.560259 | 2:-3653491 | MsG0280006579.01.T01:CDS |
| GGAATTGCAAAGAAGCTCAA+AGG | 0.567048 | 2:-3652649 | MsG0280006579.01.T01:CDS |
| GATGGCTCAAGACAGCCCAC+TGG | 0.577022 | 2:-3652715 | MsG0280006579.01.T01:CDS |
| TGGAATGTTTGAGATAGAAA+AGG | 0.585194 | 2:-3652779 | MsG0280006579.01.T01:CDS |
| GCCTGAAATATTGCCAGAAG+AGG | 0.587723 | 2:-3653649 | MsG0280006579.01.T01:CDS |
| TACAGCACTGCTTGTCAAGG+AGG | 0.588851 | 2:-3653429 | MsG0280006579.01.T01:CDS |
| AGCACCAAATGTGTATCCAA+CGG | 0.594733 | 2:+3652801 | None:intergenic |
| TTGAGCCAGTTCTAATCCAG+TGG | 0.603213 | 2:+3652699 | None:intergenic |
| AGCACTGCTTGTCAAGGAGG+TGG | 0.606846 | 2:-3653426 | MsG0280006579.01.T01:CDS |
| TTTGAGATAGAAAAGGTGAA+AGG | 0.612381 | 2:-3652772 | MsG0280006579.01.T01:CDS |
| AAGGTGTAGAAGCAAAACTC+TGG | 0.618036 | 2:-3653789 | MsG0280006579.01.T01:CDS |
| TGTCTCTATAGAAACCAAAA+GGG | 0.625559 | 2:+3654052 | None:intergenic |
| ATGGTGCTGGAACTTATGCA+GGG | 0.634036 | 2:-3652738 | MsG0280006579.01.T01:CDS |
| ATTACACAACTTGTTCACCA+TGG | 0.638391 | 2:-3652838 | MsG0280006579.01.T01:CDS |
| TGAGCCAGTTCTAATCCAGT+GGG | 0.641145 | 2:+3652700 | None:intergenic |
| GAGATAGAAAAGGTGAAAGG+TGG | 0.645866 | 2:-3652769 | MsG0280006579.01.T01:CDS |
| TGGTGCTGGAACTTATGCAG+GGG | 0.770860 | 2:-3652737 | MsG0280006579.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAGAATTCAATAATAAT+TGG | - | Chr2:3653433-3653452 | MsG0280006579.01.T01:CDS | 10.0% |
| !! | TTGTAAAATCAGAAAATAAA+TGG | + | Chr2:3653880-3653899 | None:intergenic | 15.0% |
| !!! | TAAATTAACTATTTTCGATT+TGG | - | Chr2:3653619-3653638 | MsG0280006579.01.T01:CDS | 15.0% |
| !!! | TATTGAATTCTAAAAACATT+TGG | + | Chr2:3653428-3653447 | None:intergenic | 15.0% |
| !! | CATAAAATTACTAACAAAAG+AGG | + | Chr2:3653555-3653574 | None:intergenic | 20.0% |
| !! | TTTCAAAAAATAAAGTGTGA+TGG | + | Chr2:3653397-3653416 | None:intergenic | 20.0% |
| ! | ACGTTAAATATAACTTCTGA+AGG | + | Chr2:3652849-3652868 | None:intergenic | 25.0% |
| ! | TTTAGCTACTATTCTATGTA+TGG | - | Chr2:3652897-3652916 | MsG0280006579.01.T01:intron | 25.0% |
| !! | TACTCAATTCAAAGCATTTA+TGG | - | Chr2:3653248-3653267 | MsG0280006579.01.T01:intron | 25.0% |
| !! | TTAAATATAACTTCTGAAGG+AGG | + | Chr2:3652846-3652865 | None:intergenic | 25.0% |
| ATAATTGGAACATCACTCTT+TGG | + | Chr2:3653159-3653178 | None:intergenic | 30.0% | |
| ATTCTATGTATGGACATGTT+GGG | - | Chr2:3652907-3652926 | MsG0280006579.01.T01:intron | 30.0% | |
| CATTCATTTCTAATTGATGC+GGG | + | Chr2:3653464-3653483 | None:intergenic | 30.0% | |
| CCATTCATTTCTAATTGATG+CGG | + | Chr2:3653465-3653484 | None:intergenic | 30.0% | |
| TATTCTATGTATGGACATGT+TGG | - | Chr2:3652906-3652925 | MsG0280006579.01.T01:intron | 30.0% | |
| TGTCTCTATAGAAACCAAAA+GGG | + | Chr2:3652710-3652729 | None:intergenic | 30.0% | |
| TTGCAGTTACGATACATTTA+TGG | + | Chr2:3653518-3653537 | None:intergenic | 30.0% | |
| ! | AAAGCATTTATGGATACAAC+TGG | - | Chr2:3653258-3653277 | MsG0280006579.01.T01:intron | 30.0% |
| ! | AGCTCATTTGCTGAAATAAT+TGG | + | Chr2:3653174-3653193 | None:intergenic | 30.0% |
| ! | GATACATTTATGGACTATAG+TGG | + | Chr2:3653508-3653527 | None:intergenic | 30.0% |
| ! | TGGAATGTTTGAGATAGAAA+AGG | - | Chr2:3653980-3653999 | MsG0280006579.01.T01:intron | 30.0% |
| ! | TTTGAGATAGAAAAGGTGAA+AGG | - | Chr2:3653987-3654006 | MsG0280006579.01.T01:intron | 30.0% |
| !! | TACTAACAAAAGAGGTACTA+GGG | + | Chr2:3653547-3653566 | None:intergenic | 30.0% |
| !! | TTACTAACAAAAGAGGTACT+AGG | + | Chr2:3653548-3653567 | None:intergenic | 30.0% |
| !!! | TTTGGTTTTCCAACAAGATT+TGG | - | Chr2:3653216-3653235 | MsG0280006579.01.T01:intron | 30.0% |
| AACAGCGTCTCATACATTTA+GGG | - | Chr2:3653652-3653671 | MsG0280006579.01.T01:CDS | 35.0% | |
| AATGGAGTTGAATCCATAGA+AGG | - | Chr2:3652951-3652970 | MsG0280006579.01.T01:intron | 35.0% | |
| ACAGAAGAATGAGAAACTCA+CGG | + | Chr2:3653372-3653391 | None:intergenic | 35.0% | |
| AGATTCGTGAAAATAGAGAG+AGG | + | Chr2:3652812-3652831 | None:intergenic | 35.0% | |
| CCGCATCAATTAGAAATGAA+TGG | - | Chr2:3653462-3653481 | MsG0280006579.01.T01:CDS | 35.0% | |
| CTAGCTGAAGAGATCAAAAA+TGG | - | Chr2:3652933-3652952 | MsG0280006579.01.T01:intron | 35.0% | |
| CTGTCTCTATAGAAACCAAA+AGG | + | Chr2:3652711-3652730 | None:intergenic | 35.0% | |
| GAATTGCAAAGAAGCTCAAA+GGG | - | Chr2:3654111-3654130 | MsG0280006579.01.T01:CDS | 35.0% | |
| GTGTTCAAAAGTTTACTCGT+TGG | + | Chr2:3653589-3653608 | None:intergenic | 35.0% | |
| TAACAGCGTCTCATACATTT+AGG | - | Chr2:3653651-3653670 | MsG0280006579.01.T01:CDS | 35.0% | |
| TCATCAAGGGAAATACTTTG+CGG | - | Chr2:3654088-3654107 | MsG0280006579.01.T01:CDS | 35.0% | |
| ! | ATTACACAACTTGTTCACCA+TGG | - | Chr2:3653921-3653940 | MsG0280006579.01.T01:intron | 35.0% |
| ! | CTAAGGTTTTTCTTACACTC+TGG | - | Chr2:3653782-3653801 | MsG0280006579.01.T01:intron | 35.0% |
| ! | TCCAACAAGATTTGGAATGA+TGG | - | Chr2:3653224-3653243 | MsG0280006579.01.T01:intron | 35.0% |
| ! | TGATTTTACAATGTACCTGC+AGG | - | Chr2:3653889-3653908 | MsG0280006579.01.T01:intron | 35.0% |
| ! | TTTTGCTTCTACACCTTCTA+TGG | + | Chr2:3652967-3652986 | None:intergenic | 35.0% |
| !! | TGTTTGAGAAGTGTTCAGTT+TGG | + | Chr2:3653018-3653037 | None:intergenic | 35.0% |
| AAGGTGTAGAAGCAAAACTC+TGG | - | Chr2:3652970-3652989 | MsG0280006579.01.T01:intron | 40.0% | |
| ACCTCTTCTGGCAATATTTC+AGG | + | Chr2:3653114-3653133 | None:intergenic | 40.0% | |
| AGCACCAAATGTGTATCCAA+CGG | + | Chr2:3653961-3653980 | None:intergenic | 40.0% | |
| ATATTGCCAGAAGAGGTTCT+TGG | - | Chr2:3653117-3653136 | MsG0280006579.01.T01:intron | 40.0% | |
| ATTGGAACATCACTCTTTGG+AGG | + | Chr2:3653156-3653175 | None:intergenic | 40.0% | |
| CATCAAGGGAAATACTTTGC+GGG | - | Chr2:3654089-3654108 | MsG0280006579.01.T01:CDS | 40.0% | |
| CTGAGAAATTGCTACTGCTA+AGG | - | Chr2:3653765-3653784 | MsG0280006579.01.T01:intron | 40.0% | |
| GAGATAGAAAAGGTGAAAGG+TGG | - | Chr2:3653990-3654009 | MsG0280006579.01.T01:CDS | 40.0% | |
| GCCATCATTCCAAATCTTGT+TGG | + | Chr2:3653228-3653247 | None:intergenic | 40.0% | |
| GGAATTGCAAAGAAGCTCAA+AGG | - | Chr2:3654110-3654129 | MsG0280006579.01.T01:CDS | 40.0% | |
| TATTGCCAGAAGAGGTTCTT+GGG | - | Chr2:3653118-3653137 | MsG0280006579.01.T01:intron | 40.0% | |
| TGTAATAGATGTCAACCTGC+AGG | + | Chr2:3653907-3653926 | None:intergenic | 40.0% | |
| TTCAGCAAATGAGCTTCTTG+AGG | - | Chr2:3653179-3653198 | MsG0280006579.01.T01:intron | 40.0% | |
| ! | CTTCAAGTTACTAGCAGCAA+TGG | - | Chr2:3652752-3652771 | MsG0280006579.01.T01:CDS | 40.0% |
| ! | TGGCTCAAGCTTTTCATCAA+GGG | - | Chr2:3654075-3654094 | MsG0280006579.01.T01:CDS | 40.0% |
| ! | TTTTACAGCACTGCTTGTCA+AGG | - | Chr2:3653327-3653346 | MsG0280006579.01.T01:intron | 40.0% |
| !! | GAGAAGTGTTCAGTTTGGTT+TGG | + | Chr2:3653013-3653032 | None:intergenic | 40.0% |
| !!! | GAGGCTGATGGTTTTTTGTT+TGG | - | Chr2:3653198-3653217 | MsG0280006579.01.T01:intron | 40.0% |
| AATGAGCTTCTTGAGGCTGA+TGG | - | Chr2:3653186-3653205 | MsG0280006579.01.T01:intron | 45.0% | |
| ATAGCACTTGCGGAAAGTCT+GGG | + | Chr2:3653812-3653831 | None:intergenic | 45.0% | |
| ATCTTCCCAAGAACCTCTTC+TGG | + | Chr2:3653126-3653145 | None:intergenic | 45.0% | |
| CAAGTTTCTGTGTGCACCAT+AGG | + | Chr2:3653287-3653306 | None:intergenic | 45.0% | |
| CATGACTCTGATAGCACTTG+CGG | + | Chr2:3653822-3653841 | None:intergenic | 45.0% | |
| CGGGCACAAAAATCATTCCA+TGG | + | Chr2:3653941-3653960 | None:intergenic | 45.0% | |
| GCACCAAATGTGTATCCAAC+GGG | + | Chr2:3653960-3653979 | None:intergenic | 45.0% | |
| GCATAAGTTCCAGCACCATA+TGG | + | Chr2:3654020-3654039 | None:intergenic | 45.0% | |
| GCCTGAAATATTGCCAGAAG+AGG | - | Chr2:3653110-3653129 | MsG0280006579.01.T01:intron | 45.0% | |
| TGGATACAACTGGAGACCTA+TGG | - | Chr2:3653268-3653287 | MsG0280006579.01.T01:intron | 45.0% | |
| TGTAGAAGCAAAACTCTGGC+AGG | - | Chr2:3652974-3652993 | MsG0280006579.01.T01:intron | 45.0% | |
| ! | ATGGTGCTGGAACTTATGCA+GGG | - | Chr2:3654021-3654040 | MsG0280006579.01.T01:CDS | 45.0% |
| ! | CTGGCTCAAGCTTTTCATCA+AGG | - | Chr2:3654074-3654093 | MsG0280006579.01.T01:CDS | 45.0% |
| ! | GGAATGATTTTTGTGCCCGT+TGG | - | Chr2:3653942-3653961 | MsG0280006579.01.T01:intron | 45.0% |
| ! | TATGGTGCTGGAACTTATGC+AGG | - | Chr2:3654020-3654039 | MsG0280006579.01.T01:CDS | 45.0% |
| ! | TGAGCCAGTTCTAATCCAGT+GGG | + | Chr2:3654062-3654081 | None:intergenic | 45.0% |
| ! | TTGAGCCAGTTCTAATCCAG+TGG | + | Chr2:3654063-3654082 | None:intergenic | 45.0% |
| !! | AGAAACCAAAAGGGCGTTCT+TGG | + | Chr2:3652701-3652720 | None:intergenic | 45.0% |
| !! | GTTGGATACACATTTGGTGC+TGG | - | Chr2:3653960-3653979 | MsG0280006579.01.T01:intron | 45.0% |
| !! | ATTTATTTATATATATGTAT+AGG | - | Chr2:3653086-3653105 | MsG0280006579.01.T01:intron | 5.0% |
| AAACTTGCAGGAAAGCCTGC+TGG | - | Chr2:3653300-3653319 | MsG0280006579.01.T01:intron | 50.0% | |
| CGCAAGTGCTATCAGAGTCA+TGG | - | Chr2:3653820-3653839 | MsG0280006579.01.T01:CDS | 50.0% | |
| GATAGCACTTGCGGAAAGTC+TGG | + | Chr2:3653813-3653832 | None:intergenic | 50.0% | |
| GTGAAAGGTGGCTCACCATA+TGG | - | Chr2:3654002-3654021 | MsG0280006579.01.T01:CDS | 50.0% | |
| GTGCCCGTTGGATACACATT+TGG | - | Chr2:3653954-3653973 | MsG0280006579.01.T01:intron | 50.0% | |
| GTGCTGTAAAAGATGCCAGC+AGG | + | Chr2:3653318-3653337 | None:intergenic | 50.0% | |
| TACAGCACTGCTTGTCAAGG+AGG | - | Chr2:3653330-3653349 | MsG0280006579.01.T01:intron | 50.0% | |
| TGGTGCACACAGAAACTTGC+AGG | - | Chr2:3653288-3653307 | MsG0280006579.01.T01:intron | 50.0% | |
| ! | ACAGCCCACTGGATTAGAAC+TGG | - | Chr2:3654055-3654074 | MsG0280006579.01.T01:CDS | 50.0% |
| ! | ACTCACCAAGAACGCCCTTT+TGG | - | Chr2:3652693-3652712 | MsG0280006579.01.T01:CDS | 50.0% |
| ! | TGGTGCTGGAACTTATGCAG+GGG | - | Chr2:3654022-3654041 | MsG0280006579.01.T01:CDS | 50.0% |
| AGCACTGCTTGTCAAGGAGG+TGG | - | Chr2:3653333-3653352 | MsG0280006579.01.T01:intron | 55.0% | |
| GCTGGAACTTATGCAGGGGA+TGG | - | Chr2:3654026-3654045 | MsG0280006579.01.T01:CDS | 55.0% | |
| GATGGCTCAAGACAGCCCAC+TGG | - | Chr2:3654044-3654063 | MsG0280006579.01.T01:CDS | 60.0% | |
| GGTGGCTCACCATATGGTGC+TGG | - | Chr2:3654008-3654027 | MsG0280006579.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 3652639 | 3654142 | 3652639 | ID=MsG0280006579.01;Name=MsG0280006579.01 |
| Chr2 | mRNA | 3652639 | 3654142 | 3652639 | ID=MsG0280006579.01.T01;Parent=MsG0280006579.01;Name=MsG0280006579.01.T01;_AED=0.30;_eAED=0.41;_QI=0|0|0|1|1|1|4|0|247 |
| Chr2 | exon | 3653988 | 3654142 | 3653988 | ID=MsG0280006579.01.T01:exon:1758;Parent=MsG0280006579.01.T01 |
| Chr2 | exon | 3653786 | 3653879 | 3653786 | ID=MsG0280006579.01.T01:exon:1757;Parent=MsG0280006579.01.T01 |
| Chr2 | exon | 3653411 | 3653673 | 3653411 | ID=MsG0280006579.01.T01:exon:1756;Parent=MsG0280006579.01.T01 |
| Chr2 | exon | 3652639 | 3652870 | 3652639 | ID=MsG0280006579.01.T01:exon:1755;Parent=MsG0280006579.01.T01 |
| Chr2 | CDS | 3653988 | 3654142 | 3653988 | ID=MsG0280006579.01.T01:cds;Parent=MsG0280006579.01.T01 |
| Chr2 | CDS | 3653786 | 3653879 | 3653786 | ID=MsG0280006579.01.T01:cds;Parent=MsG0280006579.01.T01 |
| Chr2 | CDS | 3653411 | 3653673 | 3653411 | ID=MsG0280006579.01.T01:cds;Parent=MsG0280006579.01.T01 |
| Chr2 | CDS | 3652639 | 3652870 | 3652639 | ID=MsG0280006579.01.T01:cds;Parent=MsG0280006579.01.T01 |
| Gene Sequence |
| Protein sequence |
