AlfalfaGEDB | MsG0380015766.01

M. sativa cultivar ZhongmuNo.1 / MsG0380015766.01

KEGG: K02900
GO: -
PFAM: PF00828

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015766.01.T01	RHN68732.1	98.81	84	1	0	1	84	44	127	1.08E-52	172

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015766.01.T01	P49637	91.667	84	7	0	1	84	1	84	9.67E-52	161

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015766.01.T01	A0A5N6NIV1	90.476	84	8	0	1	84	946	1029	1.29e-48	174

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049041.01	MsG0380015766.01	0.841315	5.120388e-58	7.494022e-55
MsG0180000096.01	MsG0380015766.01	0.802775	4.779617e-49	2.361601e-46
MsG0180000118.01	MsG0380015766.01	0.854908	8.995896e-62	2.040470e-58
MsG0180001169.01	MsG0380015766.01	0.822181	2.717525e-53	2.257807e-50
MsG0180001829.01	MsG0380015766.01	0.847723	9.655593e-60	1.731862e-56
MsG0180003897.01	MsG0380015766.01	0.886005	4.979841e-72	3.494063e-68
MsG0180004503.01	MsG0380015766.01	0.887794	1.043472e-72	7.874310e-69
MsG0180004829.01	MsG0380015766.01	0.844283	8.319756e-59	1.336619e-55
MsG0180005384.01	MsG0380015766.01	0.814992	1.161350e-51	7.910085e-49
MsG0380015766.01	MsG0380015857.01	0.863510	2.364439e-64	7.185821e-61
MsG0380015766.01	MsG0380016653.01	0.873907	1.021716e-67	4.508747e-64
MsG0380015766.01	MsG0380016732.01	0.858617	7.284333e-63	1.870238e-59
MsG0380015766.01	MsG0380016744.01	0.837755	4.309144e-57	5.653250e-54
MsG0380015766.01	MsG0380016760.01	0.806040	9.977967e-50	5.361069e-47
MsG0380015766.01	MsG0380016805.01	0.812498	4.112711e-51	2.619149e-48
MsG0380015766.01	MsG0380017664.01	0.818988	1.470455e-52	1.117676e-49
MsG0380015766.01	MsG0480018164.01	0.833171	6.215673e-56	7.101443e-53
MsG0380015766.01	MsG0480020796.01	0.885585	7.164933e-72	4.946199e-68
MsG0380015766.01	MsG0480021279.01	0.856621	2.842745e-62	6.825709e-59
MsG0380015766.01	MsG0480021417.01	0.817008	4.119151e-52	2.964818e-49
MsG0380015766.01	MsG0480022006.01	0.854523	1.163395e-61	2.605630e-58
MsG0380015766.01	MsG0480022955.01	0.891042	5.698643e-74	4.915677e-70
MsG0380015766.01	MsG0480023380.01	0.853928	1.727289e-61	3.793314e-58
MsG0380015766.01	MsG0480023774.01	0.801041	1.085583e-48	5.133028e-46
MsG0380015766.01	MsG0480023996.01	0.836764	7.727461e-57	9.834548e-54
MsG0380015766.01	MsG0580024540.01	0.807721	4.401100e-50	2.469477e-47
MsG0380015766.01	MsG0580024738.01	0.849224	3.708978e-60	6.984067e-57
MsG0380015766.01	MsG0580024894.01	0.818312	2.093282e-52	1.561442e-49
MsG0380015766.01	MsG0680031368.01	0.812541	4.025014e-51	2.566279e-48
MsG0380015766.01	MsG0780037290.01	0.821939	3.091848e-53	2.551237e-50
MsG0380015766.01	MsG0780039937.01	0.830759	2.450366e-55	2.606152e-52
MsG0380015766.01	MsG0780041311.01	0.911767	4.185108e-83	9.110899e-79
MsG0380015766.01	MsG0880042517.01	0.828277	9.832102e-55	9.726814e-52
MsG0380015766.01	MsG0880043076.01	0.809222	2.104657e-50	1.228651e-47
MsG0380015766.01	MsG0880044204.01	0.824904	6.269705e-54	5.628858e-51
MsG0280006629.01	MsG0380015766.01	0.816044	6.774465e-52	4.749002e-49
MsG0280006747.01	MsG0380015766.01	0.865156	7.242193e-65	2.332876e-61
MsG0280007055.01	MsG0380015766.01	0.862280	5.667572e-64	1.650604e-60
MsG0280010232.01	MsG0380015766.01	0.858259	9.311343e-63	2.362218e-59

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015766.01.T01	MTR_5g033090	98.810	84	1	0	1	84	1	84	1.97e-56	171
MsG0380015766.01.T01	MTR_3g077050	98.810	84	1	0	1	84	1	84	2.24e-56	170
MsG0380015766.01.T01	MTR_8g069460	88.095	84	10	0	1	84	1	84	8.62e-51	156
MsG0380015766.01.T01	MTR_4g119380	47.619	84	7	2	1	84	100	146	1.10e-13	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015766.01.T01	AT1G70600	91.667	84	7	0	1	84	1	84	9.86e-53	161
MsG0380015766.01.T01	AT1G23290	88.095	84	10	0	1	84	1	84	1.25e-50	156
MsG0380015766.01.T01	AT1G12960	56.098	82	17	1	1	82	1	63	2.30e-22	83.2

Find 29 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCTTTCTCCTTCACATCT+TGG	0.343506	3:-74792704	None:intergenic
AGCTTTCTCCTTCACATCTT+GGG	0.364212	3:-74792703	None:intergenic
ATCTTGTCGATGTTGACAAT+AGG	0.419901	3:-74792667	None:intergenic
TCTTGTCGATGTTGACAATA+GGG	0.425312	3:-74792666	None:intergenic
TCCGGCGTTACCACGACCTC+CGG	0.443108	3:-74792542	None:intergenic
CGCAAGCATCCCGGAGGTCG+TGG	0.447863	3:+74792532	MsG0380015766.01.T01:CDS
CTTCCTGTTCTTCTTGAAGC+GGG	0.447997	3:-74792464	None:intergenic
CGACCCGCTTCAAGAAGAAC+AGG	0.481196	3:+74792461	MsG0380015766.01.T01:CDS
ACGGTGATGGTGCATTCCTC+CGG	0.496860	3:-74792560	None:intergenic
CCAACCTTACCGAAGTAACC+TGG	0.506573	3:-74792604	None:intergenic
GATACTTGTCGAAGAGGATA+CGG	0.513304	3:-74792579	None:intergenic
CTCTCATTCCCAAGATGTGA+AGG	0.526318	3:+74792695	MsG0380015766.01.T01:CDS
CCAGGTTACTTCGGTAAGGT+TGG	0.536880	3:+74792604	MsG0380015766.01.T01:CDS
TCTTCCTGTTCTTCTTGAAG+CGG	0.538920	3:-74792465	None:intergenic
GGTCACGTGAGCGCCGGTCA+TGG	0.538972	3:+74792493	MsG0380015766.01.T01:CDS
TCATCCAGGTTACTTCGGTA+AGG	0.549171	3:+74792600	MsG0380015766.01.T01:CDS
CTCTTCGACAAGTATCATCC+AGG	0.553418	3:+74792586	MsG0380015766.01.T01:CDS
CTTTCCGATACGACCATGAC+CGG	0.562794	3:-74792506	None:intergenic
CCCGGAGGTCGTGGTAACGC+CGG	0.572163	3:+74792541	MsG0380015766.01.T01:CDS
AAGAGAGGTCACGTGAGCGC+CGG	0.573118	3:+74792487	MsG0380015766.01.T01:CDS
CCGGCGTTACCACGACCTCC+GGG	0.573655	3:-74792541	None:intergenic
AGCGCCGGTCATGGTCGTAT+CGG	0.577785	3:+74792502	MsG0380015766.01.T01:CDS
AAGTATCATCCAGGTTACTT+CGG	0.578842	3:+74792595	MsG0380015766.01.T01:CDS
GGAAAGCATCGCAAGCATCC+CGG	0.586004	3:+74792523	MsG0380015766.01.T01:CDS
AAGAAGAACAGGAAGAAGAG+AGG	0.594785	3:+74792472	MsG0380015766.01.T01:CDS
CTGGATGATACTTGTCGAAG+AGG	0.597192	3:-74792585	None:intergenic
GGAGGTCGTGGTAACGCCGG+AGG	0.620157	3:+74792544	MsG0380015766.01.T01:CDS
TGTCGAAGAGGATACGGTGA+TGG	0.642158	3:-74792573	None:intergenic
AAGCATCGCAAGCATCCCGG+AGG	0.718770	3:+74792526	MsG0380015766.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTATCATCCAGGTTACTT+CGG	+	Chr3:74792595-74792614	MsG0380015766.01.T01:CDS	35.0%
	ATCTTGTCGATGTTGACAAT+AGG	-	Chr3:74792670-74792689	None:intergenic	35.0%
	TCTTGTCGATGTTGACAATA+GGG	-	Chr3:74792669-74792688	None:intergenic	35.0%
	AAGAAGAACAGGAAGAAGAG+AGG	+	Chr3:74792472-74792491	MsG0380015766.01.T01:CDS	40.0%
	GATACTTGTCGAAGAGGATA+CGG	-	Chr3:74792582-74792601	None:intergenic	40.0%
	TCTTCCTGTTCTTCTTGAAG+CGG	-	Chr3:74792468-74792487	None:intergenic	40.0%
	CTCTTCGACAAGTATCATCC+AGG	+	Chr3:74792586-74792605	MsG0380015766.01.T01:CDS	45.0%
	CTGGATGATACTTGTCGAAG+AGG	-	Chr3:74792588-74792607	None:intergenic	45.0%
	CTTCCTGTTCTTCTTGAAGC+GGG	-	Chr3:74792467-74792486	None:intergenic	45.0%
	TCATCCAGGTTACTTCGGTA+AGG	+	Chr3:74792600-74792619	MsG0380015766.01.T01:CDS	45.0%
	CCAACCTTACCGAAGTAACC+TGG	-	Chr3:74792607-74792626	None:intergenic	50.0%
	CCAGGTTACTTCGGTAAGGT+TGG	+	Chr3:74792604-74792623	MsG0380015766.01.T01:CDS	50.0%
	CTTTCCGATACGACCATGAC+CGG	-	Chr3:74792509-74792528	None:intergenic	50.0%
	TGTCGAAGAGGATACGGTGA+TGG	-	Chr3:74792576-74792595	None:intergenic	50.0%
	CGACCCGCTTCAAGAAGAAC+AGG	+	Chr3:74792461-74792480	MsG0380015766.01.T01:CDS	55.0%
!	ACGGTGATGGTGCATTCCTC+CGG	-	Chr3:74792563-74792582	None:intergenic	55.0%
!!	GGAAAGCATCGCAAGCATCC+CGG	+	Chr3:74792523-74792542	MsG0380015766.01.T01:CDS	55.0%
	AAGAGAGGTCACGTGAGCGC+CGG	+	Chr3:74792487-74792506	MsG0380015766.01.T01:CDS	60.0%
!	AAGCATCGCAAGCATCCCGG+AGG	+	Chr3:74792526-74792545	MsG0380015766.01.T01:CDS	60.0%
!!	AGCGCCGGTCATGGTCGTAT+CGG	+	Chr3:74792502-74792521	MsG0380015766.01.T01:CDS	60.0%
	TCCGGCGTTACCACGACCTC+CGG	-	Chr3:74792545-74792564	None:intergenic	65.0%
	CCGGCGTTACCACGACCTCC+GGG	-	Chr3:74792544-74792563	None:intergenic	70.0%
	CGCAAGCATCCCGGAGGTCG+TGG	+	Chr3:74792532-74792551	MsG0380015766.01.T01:CDS	70.0%
!	CCCGGAGGTCGTGGTAACGC+CGG	+	Chr3:74792541-74792560	MsG0380015766.01.T01:CDS	70.0%
!	GGAGGTCGTGGTAACGCCGG+AGG	+	Chr3:74792544-74792563	MsG0380015766.01.T01:CDS	70.0%
!!	GGTCACGTGAGCGCCGGTCA+TGG	+	Chr3:74792493-74792512	MsG0380015766.01.T01:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	74792457	74792714	74792457	ID=MsG0380015766.01;Name=MsG0380015766.01
Chr3	mRNA	74792457	74792714	74792457	ID=MsG0380015766.01.T01;Parent=MsG0380015766.01;Name=MsG0380015766.01.T01;_AED=0.32;_eAED=0.32;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|85
Chr3	exon	74792457	74792714	74792457	ID=MsG0380015766.01.T01:exon:3780;Parent=MsG0380015766.01.T01
Chr3	CDS	74792457	74792714	74792457	ID=MsG0380015766.01.T01:cds;Parent=MsG0380015766.01.T01

Gene Sequence	>MsG0380015766.01.T01 ATGACGACCCGCTTCAAGAAGAACAGGAAGAAGAGAGGTCACGTGAGCGCCGGTCATGGTCGTATCGGAAAGCATCGCAAGCATCCCGGAGGTCGTGGTAACGCCGGAGGAATGCACCATCACCGTATCCTCTTCGACAAGTATCATCCAGGTTACTTCGGTAAGGTTGGTATGCGTTACTTTCACAAGCTTCGTAACAAATTCTATAACCCTATTGTCAACATCGACAAGATCGCTTCTCTCATTCCCAAGATGTGA
Protein sequence	>MsG0380015766.01.T01 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPKM*