AlfalfaGEDB | MsG0580029283.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029283.01

KEGG: K14840
GO: -
PFAM: PF07767

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029283.01.T01	XP_003616741.1	98.634	366	3	1	1	364	1	366	0	723

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029283.01.T01	O22892	54.217	249	103	6	1	246	1	241	9.08E-69	225

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029283.01.T01	G7K7B9	98.634	366	3	1	1	364	1	366	0.0	723

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0580029283.01	0.805928	1.053457e-49	5.643972e-47
MsG0080048197.01	MsG0580029283.01	0.828532	8.531460e-55	8.503335e-52
MsG0080048383.01	MsG0580029283.01	0.831789	1.367461e-55	1.499691e-52
MsG0080048687.01	MsG0580029283.01	0.834946	2.233773e-56	2.691650e-53
MsG0080048784.01	MsG0580029283.01	0.831764	1.387168e-55	1.520166e-52
MsG0080048843.01	MsG0580029283.01	0.843108	1.715800e-58	2.656501e-55
MsG0080048968.01	MsG0580029283.01	0.924504	6.446600e-90	2.733302e-85
MsG0080049135.01	MsG0580029283.01	0.833309	5.742239e-56	6.587666e-53
MsG0180000023.01	MsG0580029283.01	0.812643	3.823600e-51	2.444279e-48
MsG0180000055.01	MsG0580029283.01	0.857691	1.373196e-62	3.418294e-59

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PPI

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Gene1	Gene2	Type
MsG0280009716.01	MsG0580029283.01	PPI
MsG0580029283.01	MsG0180002023.01	PPI
MsG0580029283.01	MsG0180005851.01	PPI
MsG0580029283.01	MsG0380016513.01	PPI
MsG0580029283.01	MsG0580025421.01	PPI
MsG0580029283.01	MsG0780037159.01	PPI
MsG0580029283.01	MsG0380017788.01	PPI
MsG0580029283.01	MsG0180005851.01	PPI
MsG0580029283.01	MsG0380016248.01	PPI
MsG0580029283.01	MsG0480022791.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029283.01.T01	MTR_5g083780	98.634	366	3	1	1	364	1	366	0.0	723

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029283.01.T01	AT2G40430	54.217	249	103	6	1	246	1	241	8.48e-70	226
MsG0580029283.01.T01	AT2G40430	52.941	170	79	1	197	366	275	443	3.40e-42	153
MsG0580029283.01.T01	AT2G40430	54.217	249	103	6	1	246	1	241	9.26e-70	225
MsG0580029283.01.T01	AT2G40430	52.071	169	80	1	197	365	275	442	6.36e-41	149
MsG0580029283.01.T01	AT2G40430	53.704	162	74	1	197	358	126	286	3.14e-42	149
MsG0580029283.01.T01	AT2G40430	73.563	87	23	0	1	87	1	87	5.10e-35	130

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Find 70 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGTATTGTTAGAAAA+TGG	0.156439	5:-94707414	MsG0580029283.01.T01:exon
AAAGCCCAGGAGAAACAGGT+TGG	0.580924	5:-94703816	MsG0580029283.01.T01:CDS
AAAGTCTACAGAAATGAATT+GGG	0.481927	5:-94705275	MsG0580029283.01.T01:CDS
AAAGTGTATTGTTAGAAAAT+GGG	0.342504	5:-94707413	MsG0580029283.01.T01:exon
AAATACGTCAGAATCTAACA+CGG	0.731068	5:+94705765	None:intergenic
AAATCGAAAAGTTCAAGGAA+GGG	0.576425	5:-94707386	MsG0580029283.01.T01:CDS
AAATGAATTGGGCCCTGAAC+CGG	0.418925	5:-94705264	MsG0580029283.01.T01:CDS
AACCTGTTTCTCCTGGGCTT+TGG	0.342084	5:+94703818	None:intergenic
AAGCATAGAGAAGAGAGGGT+TGG	0.525558	5:-94703846	MsG0580029283.01.T01:CDS
AAGGCACTGGCTTAACAAAC+TGG	0.432795	5:+94706035	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACGCAATTGTTGTAGAAA+TGG	+	Chr5:94707143-94707162	None:intergenic	30.0%
AAATACGTCAGAATCTAACA+CGG	+	Chr5:94705496-94705515	None:intergenic	30.0%
AAATCGAAAAGTTCAAGGAA+GGG	-	Chr5:94703872-94703891	MsG0580029283.01.T01:CDS	30.0%
AACTAATGGGAATAAGATCA+AGG	+	Chr5:94704767-94704786	None:intergenic	30.0%
AAGCTATCTTAGAGAACTAA+TGG	+	Chr5:94704781-94704800	None:intergenic	30.0%
AATGTGCAGCAACAATAAAA+TGG	+	Chr5:94705701-94705720	None:intergenic	30.0%
ACATAAACTTGTACGAGAAA+TGG	-	Chr5:94704959-94704978	MsG0580029283.01.T01:intron	30.0%
ACCAATTCCATTATTGTAAC+AGG	-	Chr5:94707171-94707190	MsG0580029283.01.T01:intron	30.0%
ACTAATGGGAATAAGATCAA+GGG	+	Chr5:94704766-94704785	None:intergenic	30.0%
AGCTATCTTAGAGAACTAAT+GGG	+	Chr5:94704780-94704799	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	94703811	94707469	94703811	ID=MsG0580029283.01;Name=MsG0580029283.01
Chr5	mRNA	94703811	94707469	94703811	ID=MsG0580029283.01.T01;Parent=MsG0580029283.01;Name=MsG0580029283.01.T01;_AED=0.38;_eAED=0.38;_QI=52\|0.9\|0.81\|1\|1\|1\|11\|0\|366
Chr5	exon	94703811	94703900	94703811	ID=MsG0580029283.01.T01:exon:49952;Parent=MsG0580029283.01.T01
Chr5	exon	94704021	94704087	94704021	ID=MsG0580029283.01.T01:exon:49951;Parent=MsG0580029283.01.T01
Chr5	exon	94704228	94704350	94704228	ID=MsG0580029283.01.T01:exon:49950;Parent=MsG0580029283.01.T01
Chr5	exon	94704451	94704570	94704451	ID=MsG0580029283.01.T01:exon:49949;Parent=MsG0580029283.01.T01
Chr5	exon	94704987	94705075	94704987	ID=MsG0580029283.01.T01:exon:49948;Parent=MsG0580029283.01.T01
Chr5	exon	94705220	94705333	94705220	ID=MsG0580029283.01.T01:exon:49947;Parent=MsG0580029283.01.T01
Chr5	exon	94705432	94705512	94705432	ID=MsG0580029283.01.T01:exon:49946;Parent=MsG0580029283.01.T01
Chr5	exon	94705626	94705651	94705626	ID=MsG0580029283.01.T01:exon:49945;Parent=MsG0580029283.01.T01
Chr5	exon	94705746	94705794	94705746	ID=MsG0580029283.01.T01:exon:49944;Parent=MsG0580029283.01.T01
Chr5	exon	94705958	94706130	94705958	ID=MsG0580029283.01.T01:exon:49943;Parent=MsG0580029283.01.T01
Chr5	exon	94707249	94707469	94707249	ID=MsG0580029283.01.T01:exon:49942;Parent=MsG0580029283.01.T01
Chr5	five_prime_UTR	94707418	94707469	94707418	ID=MsG0580029283.01.T01:five_prime_utr;Parent=MsG0580029283.01.T01
Chr5	CDS	94707249	94707417	94707249	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94705958	94706130	94705958	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94705746	94705794	94705746	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94705626	94705651	94705626	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94705432	94705512	94705432	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94705220	94705333	94705220	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94704987	94705075	94704987	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94704451	94704570	94704451	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94704228	94704350	94704228	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94704021	94704087	94704021	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01
Chr5	CDS	94703811	94703900	94703811	ID=MsG0580029283.01.T01:cds;Parent=MsG0580029283.01.T01

Gene Sequence

>MsG0580029283.01.T01

ATGGGGAAGAAATCGAAAAGTTCAAGGAAGGGGAAGAAAGCATGGAGAGCTAACATAAGCACAGAAGAAATCGAAGATTTCTTTGAGAAATCAACAAAAGATGCTCTTTCTGGTGGTTCTCTTCAAGCTGTTTCCAGTGATTCTCTCTTTTTCGAAGACAAATCAAAAGATCTTGCGGTAAAGAAGAAGATCGAAAAACATAGAGAAAAAGTGCTTCATGTTGATAGCATACTACAAAAAAACCAGTTTGTTAAGCCAGTGCCTTCATCTACTCTAAAGAAACATGCGAAAAACCGGAAAGTGGTGTCCAAAAGTTTAGTCGCCAATGAAAATATTCATAAGGAGGGTTCCGTGTTAGATTCTGACGTATTTGATATGTGGGGTGATAAAGATGAAGGCAACAAGAAAGTGAAAAAGTTGTGCAAGCCTACTCATATTCCAGCTGTCGAGGTTGATCCTCCAGGATGCTCATTTAATCCCACACATGAAAGTCATCAGGATACATTAGCTACTGCTGTTGCGGAAGAAATGATTAAAGTCTACAGAAATGAATTGGGCCCTGAACCGGTGCCTTTAACTGTTCCTGCTGGAGAAGCTATTTCTGAAGAAGATATGTATTTTCTTGATGTGGAGAAGAGTGACGATGATGAAAGTAATCACGAAGATGATGGTGAAAATGAAGACGATGCGTCAGAGAAAAAGCCCATTAAAACAAAGAAAGTGACTAGAGTTGAGTTGAATAAGAGAATTCGGCGCAGAGAACAATTGAAAAAAGAAGCTGAATCAAAGAAGAAGAAGAATTTCTCAAAAGAAATTGATAGCATCTCAAAAATTGCCGAGGAAATTAAGCATGAAGATGAGGAAAAATCCAGAAGACATCTGAGGCGACAAGTTGCTAAACAAGAGAGGTTAAAGGCATGTCCTCCTCGCTTAGGAAAGCACAAGTTTCAGCCTGCTCCTGTTCAAGTTTTATTAAGCGAAGAGATAAATGGATCCATCCGAAAGCTTAAGGGTTGTTGCACCCTTCTAAAGGATCGGTATAAAAGCATAGAGAAGAGAGGGTTGGTTGTTCCAAAGCCCAGGAGAAACAGGTTGGTTTAA

Protein sequence

>MsG0580029283.01.T01

MGKKSKSSRKGKKAWRANISTEEIEDFFEKSTKDALSGGSLQAVSSDSLFFEDKSKDLAVKKKIEKHREKVLHVDSILQKNQFVKPVPSSTLKKHAKNRKVVSKSLVANENIHKEGSVLDSDVFDMWGDKDEGNKKVKKLCKPTHIPAVEVDPPGCSFNPTHESHQDTLATAVAEEMIKVYRNELGPEPVPLTVPAGEAISEEDMYFLDVEKSDDDESNHEDDGENEDDASEKKPIKTKKVTRVELNKRIRRREQLKKEAESKKKKNFSKEIDSISKIAEEIKHEDEEKSRRHLRRQVAKQERLKACPPRLGKHKFQPAPVQVLLSEEINGSIRKLKGCCTLLKDRYKSIEKRGLVVPKPRRNRLV*