AlfalfaGEDB | MsG0780036088.01

M. sativa cultivar ZhongmuNo.1 / MsG0780036088.01

KEGG: -
GO: GO:0005515
PFAM: PF00646 PF07723

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036088.01.T01	KEH21552.1	69.883	342	60	3	1	299	1	342	1.16E-160	461

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036088.01.T01	Q9LXJ6	31.188	202	92	5	1	158	6	204	1.40E-14	77

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036088.01.T01	A0A072TVT1	69.883	342	60	3	1	299	1	342	5.53e-161	461

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048156.01	MsG0780036088.01	PPI
MsG0080049065.01	MsG0780036088.01	PPI
MsG0180005012.01	MsG0780036088.01	PPI
MsG0180005013.01	MsG0780036088.01	PPI
MsG0180006146.01	MsG0780036088.01	PPI
MsG0280007849.01	MsG0780036088.01	PPI
MsG0280008687.01	MsG0780036088.01	PPI
MsG0280008689.01	MsG0780036088.01	PPI
MsG0280011351.01	MsG0780036088.01	PPI
MsG0280011360.01	MsG0780036088.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036088.01.T01	MTR_7g007370	69.883	342	60	3	1	299	1	342	1.40e-164	461
MsG0780036088.01.T01	MTR_7g007340	57.895	266	84	4	4	249	5	262	3.60e-90	273
MsG0780036088.01.T01	MTR_3g099830	49.643	280	105	4	1	249	1	275	5.37e-70	221
MsG0780036088.01.T01	MTR_2g007400	46.644	298	94	8	16	250	11	306	1.12e-63	206
MsG0780036088.01.T01	MTR_2g005990	42.953	298	109	5	12	249	1	297	3.80e-61	199
MsG0780036088.01.T01	MTR_2g007360	44.840	281	114	9	1	250	1	271	2.89e-58	191
MsG0780036088.01.T01	MTR_2g008320	41.472	299	116	8	5	249	44	337	4.22e-53	179
MsG0780036088.01.T01	MTR_2g008310	41.958	286	111	9	16	249	1	283	2.21e-51	174
MsG0780036088.01.T01	MTR_5g083890	38.361	305	121	6	12	249	1	305	3.79e-49	171
MsG0780036088.01.T01	MTR_1g107385	35.484	310	133	8	5	249	21	328	1.20e-45	159

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036088.01.T01	AT3G52680	31.188	202	92	5	1	158	6	204	4.00e-16	78.2
MsG0780036088.01.T01	AT3G52680	31.188	202	92	5	1	158	6	204	1.43e-15	77.0
MsG0780036088.01.T01	AT5G53840	31.522	184	81	6	15	157	16	195	9.06e-14	71.6
MsG0780036088.01.T01	AT3G26922	33.333	162	83	6	17	158	14	170	2.79e-11	63.2
MsG0780036088.01.T01	AT2G04230	60.000	45	18	0	17	61	13	57	8.05e-11	62.8

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Find 63 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGGTGAAGGCTATGGAGG+AGG	0.549192	7:-2297063	MsG0780036088.01.T01:CDS
AACTTCAACGGTGAAGGCTA+TGG	0.505115	7:-2297069	MsG0780036088.01.T01:CDS
AAGATAGTATTAGGAAATTC+AGG	0.207093	7:+2296816	None:intergenic
AAGGTATTTGGTTACCGATT+TGG	0.304905	7:+2296893	None:intergenic
AAGGTCAAGAGTTTGAAGCT+TGG	0.581524	7:+2296287	None:intergenic
ACGATAGTTGAGCACCAAAT+GGG	0.529891	7:+2296344	None:intergenic
ACTCAATGTCTTTCAGATGT+AGG	0.422872	7:+2296700	None:intergenic
ACTGAGCTTAAGAATAACAA+GGG	0.598793	7:+2296782	None:intergenic
AGATAGTATTAGGAAATTCA+GGG	0.547861	7:+2296817	None:intergenic
AGATCTTGATTGTCTTCTAG+AGG	0.574157	7:-2296664	MsG0780036088.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCCAACAATTTCATGGT+TGG	+	Chr7:2296748-2296767	None:intergenic	30.0%
AACCAAATCCAACAATTTCA+TGG	+	Chr7:2296752-2296771	None:intergenic	30.0%
AACCATGAAATTGTTGGATT+TGG	-	Chr7:2296747-2296766	MsG0780036088.01.T01:CDS	30.0%
ACTGAGCTTAAGAATAACAA+GGG	+	Chr7:2295777-2295796	None:intergenic	30.0%
AGTTGTAGGTAATCATTACA+TGG	-	Chr7:2295697-2295716	MsG0780036088.01.T01:intron	30.0%
ATGTGAACTAGGATATCATT+TGG	+	Chr7:2295542-2295561	None:intergenic	30.0%
CAAAGAGATGGAAGAATTTA+TGG	-	Chr7:2295609-2295628	MsG0780036088.01.T01:intron	30.0%
CATACAGGTATGTTAAGAAA+AGG	+	Chr7:2296345-2296364	None:intergenic	30.0%
GACATTGAGTTTCAACAATA+TGG	-	Chr7:2295869-2295888	MsG0780036088.01.T01:intron	30.0%
GGGTCAAATTATTGAATGTA+GGG	+	Chr7:2296195-2296214	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	2295473	2297105	2295473	ID=MsG0780036088.01;Name=MsG0780036088.01
Chr7	mRNA	2295473	2297105	2295473	ID=MsG0780036088.01.T01;Parent=MsG0780036088.01;Name=MsG0780036088.01.T01;_AED=0.19;_eAED=0.19;_QI=0\|0\|0\|1\|0.5\|0.33\|3\|0\|299
Chr7	exon	2296485	2297105	2296485	ID=MsG0780036088.01.T01:exon:2349;Parent=MsG0780036088.01.T01
Chr7	exon	2296215	2296406	2296215	ID=MsG0780036088.01.T01:exon:2348;Parent=MsG0780036088.01.T01
Chr7	exon	2295473	2295559	2295473	ID=MsG0780036088.01.T01:exon:2347;Parent=MsG0780036088.01.T01
Chr7	CDS	2296485	2297105	2296485	ID=MsG0780036088.01.T01:cds;Parent=MsG0780036088.01.T01
Chr7	CDS	2296215	2296406	2296215	ID=MsG0780036088.01.T01:cds;Parent=MsG0780036088.01.T01
Chr7	CDS	2295473	2295559	2295473	ID=MsG0780036088.01.T01:cds;Parent=MsG0780036088.01.T01

Gene Sequence

>MsG0780036088.01.T01

ATGTCTCAGCCGGAAACTTCAACGGTGAAGGCTATGGAGGAGGAGGTAGATAGGATCAGCATTTTACCAAATGATATCCTAGTTCACATTCTCTCTTCTCTTCCCACCAATCAAGCCTTTGTGACTTCCATTCTTTCAAAGAGATGGAAGAATTTATGGTGTCTCGTTCCAGCTCTCGAATTCGTCGGAACCAAATCGGTAACCAAATACCTTGTTTCGGGAGAAGTTGTAGGTAATCATTACATGGATAATTGGGAAAGAATATACCCTGAATTTCCTAATACTATCTTTACTAGTGAAACCCTTGTTATTCTTAAGCTCAGTAATATTTTTATGGGTGCTGGGTTTTGTAATTATCTCATAAGATTACCCTCACTCAAAACCCTACATCTGAAAGACATTGAGTTTCAACAATATGGAGATCTTGATTGTCTTCTAGAGGGATGTCCAGTTCTTGAAGATCTTCAACTATATGTTATATCCTATGTCACTCTTCTTTATTCCAAGGCATGCTGTGAAACCTTGACAAAGTTGAATAGAGCAGATATTATTCAATGTGAATGCTGTGTTCTGATGAAAGCACTTTCAAATGTGGAGTTTCTACACATAAAACTATCCATGGGTTATCTTTATTACGTCTTCCCTACATTCAATAATTTGACCCATTTGGTGCTCAACTATCGTCACGGTATAGATCTACAGATGCTCCACCATTGCCCCAAGCTTCAAACTCTTGACCTTTATCAGGTTTGTTTCTTAAATTTAACAATTATATTTCTTTTTTTTTCCTTCCTTTTCTTAACATACCTGTATACCCCCGAAGAAAACCAAGAACTGAGCAGCATACCCAAAAATACGAAGGAAGTTGAATCTTTTTATGGCACTGGCATAACCCTGTAG

Protein sequence

>MsG0780036088.01.T01

MSQPETSTVKAMEEEVDRISILPNDILVHILSSLPTNQAFVTSILSKRWKNLWCLVPALEFVGTKSVTKYLVSGEVVGNHYMDNWERIYPEFPNTIFTSETLVILKLSNIFMGAGFCNYLIRLPSLKTLHLKDIEFQQYGDLDCLLEGCPVLEDLQLYVISYVTLLYSKACCETLTKLNRADIIQCECCVLMKALSNVEFLHIKLSMGYLYYVFPTFNNLTHLVLNYRHGIDLQMLHHCPKLQTLDLYQVCFLNLTIIFLFFSFLFLTYLYTPEENQELSSIPKNTKEVESFYGTGITL*