Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005012.01.T01 | KEH43362.1 | 78.893 | 289 | 20 | 1 | 1 | 289 | 164 | 411 | 4.74E-155 | 449 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005012.01.T01 | Q9M1Q1 | 27.586 | 290 | 194 | 9 | 8 | 287 | 176 | 459 | 5.19E-25 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005012.01.T01 | A0A072VNK9 | 78.893 | 289 | 20 | 1 | 1 | 289 | 164 | 411 | 2.27e-155 | 449 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005012.01.T01 | MTR_1g090903 | 78.893 | 289 | 20 | 1 | 1 | 289 | 164 | 411 | 5.74e-159 | 449 |
| MsG0180005012.01.T01 | MTR_1g090907 | 65.878 | 296 | 88 | 5 | 1 | 289 | 166 | 455 | 1.17e-127 | 371 |
| MsG0180005012.01.T01 | MTR_2g048140 | 65.085 | 295 | 89 | 6 | 3 | 289 | 168 | 456 | 4.61e-126 | 367 |
| MsG0180005012.01.T01 | MTR_3g448340 | 31.399 | 293 | 184 | 8 | 5 | 283 | 165 | 454 | 1.19e-34 | 130 |
| MsG0180005012.01.T01 | MTR_5g080150 | 30.531 | 226 | 125 | 4 | 5 | 228 | 170 | 365 | 3.31e-26 | 106 |
| MsG0180005012.01.T01 | MTR_3g049530 | 29.126 | 309 | 180 | 8 | 9 | 288 | 118 | 416 | 1.22e-25 | 105 |
| MsG0180005012.01.T01 | MTR_7g029260 | 38.739 | 111 | 67 | 1 | 152 | 261 | 49 | 159 | 2.30e-16 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005012.01.T01 | AT3G62230 | 27.586 | 290 | 194 | 9 | 8 | 287 | 176 | 459 | 5.29e-26 | 107 |
| MsG0180005012.01.T01 | AT5G54820 | 28.623 | 276 | 168 | 8 | 4 | 259 | 172 | 438 | 9.38e-26 | 106 |
| MsG0180005012.01.T01 | AT1G56400 | 26.357 | 258 | 169 | 7 | 4 | 247 | 134 | 384 | 5.87e-21 | 92.4 |
| MsG0180005012.01.T01 | AT1G56400 | 26.357 | 258 | 169 | 7 | 4 | 247 | 175 | 425 | 9.39e-21 | 92.0 |
| MsG0180005012.01.T01 | AT3G29830 | 29.304 | 273 | 163 | 13 | 4 | 257 | 170 | 431 | 1.17e-17 | 83.2 |
| MsG0180005012.01.T01 | AT3G29830 | 27.839 | 273 | 168 | 12 | 4 | 257 | 170 | 432 | 6.73e-16 | 77.8 |
| MsG0180005012.01.T01 | AT2G17305 | 33.600 | 125 | 75 | 4 | 146 | 269 | 127 | 244 | 1.35e-13 | 69.7 |
Find 42 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTAAATACTTCAAGTATTC+TGG | 0.119443 | 1:-85655764 | MsG0180005012.01.T01:CDS |
| TTACAGACCTTGAGAAATTT+TGG | 0.162868 | 1:-85655130 | MsG0180005012.01.T01:CDS |
| AAGTCAACAAGTTATATTTA+AGG | 0.205513 | 1:-85655296 | MsG0180005012.01.T01:intron |
| TGCACTCAAAGACCTTTCTT+TGG | 0.273300 | 1:-85655975 | MsG0180005012.01.T01:CDS |
| AGCAACAAGTCCTAAGTTCT+TGG | 0.287063 | 1:+85655030 | None:intergenic |
| TTTCTCAAGGTCTGTAAGAT+AGG | 0.287636 | 1:+85655136 | None:intergenic |
| TACTTGAAGTATTTAAGATT+TGG | 0.310198 | 1:+85655770 | None:intergenic |
| GCACTCAAAGACCTTTCTTT+GGG | 0.333998 | 1:-85655974 | MsG0180005012.01.T01:CDS |
| TCTGAAGAGGTGTTGGGATT+TGG | 0.338041 | 1:-85655882 | MsG0180005012.01.T01:CDS |
| TCTACCTCTTCTATAACATT+TGG | 0.363653 | 1:+85655710 | None:intergenic |
| CTCAAAGATATTGTACAACT+TGG | 0.380226 | 1:-85655848 | MsG0180005012.01.T01:CDS |
| TTAAATACTTCAAGTATTCT+GGG | 0.388380 | 1:-85655763 | MsG0180005012.01.T01:CDS |
| TCTAATGAAAGGGAAAGCTT+TGG | 0.395771 | 1:-85655006 | MsG0180005012.01.T01:CDS |
| GCACCAAAGTTAAGAAAATT+CGG | 0.399622 | 1:+85655995 | None:intergenic |
| ATGCATAAATATGAGCTTTG+TGG | 0.400472 | 1:-85655373 | MsG0180005012.01.T01:CDS |
| AACAGATTAATATTAAGTTA+TGG | 0.414219 | 1:-85654983 | MsG0180005012.01.T01:CDS |
| TGCAAATTCTCTGAACGCTC+TGG | 0.416716 | 1:+85654903 | None:intergenic |
| ATTTGAGTCTGAAGAGGTGT+TGG | 0.416726 | 1:-85655889 | MsG0180005012.01.T01:CDS |
| CTTGTTGCTTTCTAATGAAA+GGG | 0.435494 | 1:-85655016 | MsG0180005012.01.T01:CDS |
| TTTGAGTCTGAAGAGGTGTT+GGG | 0.435529 | 1:-85655888 | MsG0180005012.01.T01:CDS |
| TCAAAGACCTTTCTTTGGGT+TGG | 0.435585 | 1:-85655970 | MsG0180005012.01.T01:CDS |
| ATTAAGTTATGGAATGAAGA+TGG | 0.439836 | 1:-85654972 | MsG0180005012.01.T01:CDS |
| AATGCTTGCAAAATACTTTG+AGG | 0.447199 | 1:-85655085 | MsG0180005012.01.T01:CDS |
| GAAGAGGTGTTGGGATTTGG+TGG | 0.462276 | 1:-85655879 | MsG0180005012.01.T01:CDS |
| TTAAGTTATGGAATGAAGAT+GGG | 0.463289 | 1:-85654971 | MsG0180005012.01.T01:CDS |
| ATGCTTGCAAAATACTTTGA+GGG | 0.470267 | 1:-85655084 | MsG0180005012.01.T01:CDS |
| ACTTGTTGCTTTCTAATGAA+AGG | 0.474911 | 1:-85655017 | MsG0180005012.01.T01:CDS |
| TTCAATAAACTTCATGCCAA+TGG | 0.482452 | 1:-85655662 | MsG0180005012.01.T01:CDS |
| GAGCCACAAGCCAAGAACTT+AGG | 0.485470 | 1:-85655040 | MsG0180005012.01.T01:CDS |
| TTCTCAAGGTCTGTAAGATA+GGG | 0.517184 | 1:+85655137 | None:intergenic |
| CTTCAATCCAACCCAAAGAA+AGG | 0.520129 | 1:+85655963 | None:intergenic |
| CAAAGATGCTAAGATTCTAA+CGG | 0.537673 | 1:-85655615 | MsG0180005012.01.T01:intron |
| AGTCCTAAGTTCTTGGCTTG+TGG | 0.551466 | 1:+85655037 | None:intergenic |
| TCAAAGATATTGTACAACTT+GGG | 0.557071 | 1:-85655847 | MsG0180005012.01.T01:CDS |
| GTCACTAGGTCACCTGCAGA+TGG | 0.558055 | 1:+85655448 | None:intergenic |
| CAGTATTTGACATAGTCCAT+TGG | 0.560520 | 1:+85655646 | None:intergenic |
| TTGAGAATTTGAGTCTGAAG+AGG | 0.567770 | 1:-85655895 | MsG0180005012.01.T01:CDS |
| GCGTTTGCCAAAATTTCTCA+AGG | 0.569849 | 1:+85655123 | None:intergenic |
| AAGTCCAAATGTTATAGAAG+AGG | 0.593335 | 1:-85655714 | MsG0180005012.01.T01:CDS |
| TAAATACTTCAAGTATTCTG+GGG | 0.613240 | 1:-85655762 | MsG0180005012.01.T01:CDS |
| GTAAACGTTGTGCACGTCGA+CGG | 0.623132 | 1:+85654934 | None:intergenic |
| TAAGTTATGGAATGAAGATG+GGG | 0.628981 | 1:-85654970 | MsG0180005012.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAGATTAATATTAAGTTA+TGG | - | Chr1:85655902-85655921 | MsG0180005012.01.T01:CDS | 15.0% |
| !! | AAGTCAACAAGTTATATTTA+AGG | - | Chr1:85655589-85655608 | MsG0180005012.01.T01:intron | 20.0% |
| !! | TTAAATACTTCAAGTATTCT+GGG | - | Chr1:85655122-85655141 | MsG0180005012.01.T01:CDS | 20.0% |
| !!! | TACAGTGATATTTGTTATTA+AGG | - | Chr1:85655368-85655387 | MsG0180005012.01.T01:CDS | 20.0% |
| !!! | TACTTGAAGTATTTAAGATT+TGG | + | Chr1:85655118-85655137 | None:intergenic | 20.0% |
| !!! | TTTATTGAATTTTGTTTCTG+TGG | + | Chr1:85655212-85655231 | None:intergenic | 20.0% |
| ! | ATCAATAAAATTAGTGTACC+TGG | + | Chr1:85655312-85655331 | None:intergenic | 25.0% |
| ! | ATTAAGTTATGGAATGAAGA+TGG | - | Chr1:85655913-85655932 | MsG0180005012.01.T01:CDS | 25.0% |
| ! | CTTAAATACTTCAAGTATTC+TGG | - | Chr1:85655121-85655140 | MsG0180005012.01.T01:CDS | 25.0% |
| ! | TAAATACTTCAAGTATTCTG+GGG | - | Chr1:85655123-85655142 | MsG0180005012.01.T01:CDS | 25.0% |
| ! | TCAAAGATATTGTACAACTT+GGG | - | Chr1:85655038-85655057 | MsG0180005012.01.T01:CDS | 25.0% |
| ! | TTAAGTTATGGAATGAAGAT+GGG | - | Chr1:85655914-85655933 | MsG0180005012.01.T01:CDS | 25.0% |
| !! | TGTTTTCTCGTATATATTAC+AGG | - | Chr1:85655714-85655733 | MsG0180005012.01.T01:CDS | 25.0% |
| !!! | TCTTTTGTTATGCACAAAAA+AGG | - | Chr1:85655409-85655428 | MsG0180005012.01.T01:CDS | 25.0% |
| AAGTCCAAATGTTATAGAAG+AGG | - | Chr1:85655171-85655190 | MsG0180005012.01.T01:intron | 30.0% | |
| AATGCTTGCAAAATACTTTG+AGG | - | Chr1:85655800-85655819 | MsG0180005012.01.T01:CDS | 30.0% | |
| ACAACTTGGGTTAAAAAGAT+TGG | - | Chr1:85655051-85655070 | MsG0180005012.01.T01:CDS | 30.0% | |
| ATGCATAAATATGAGCTTTG+TGG | - | Chr1:85655512-85655531 | MsG0180005012.01.T01:intron | 30.0% | |
| ATGCTTGCAAAATACTTTGA+GGG | - | Chr1:85655801-85655820 | MsG0180005012.01.T01:CDS | 30.0% | |
| CAAAGATGCTAAGATTCTAA+CGG | - | Chr1:85655270-85655289 | MsG0180005012.01.T01:intron | 30.0% | |
| CTCAAAGATATTGTACAACT+TGG | - | Chr1:85655037-85655056 | MsG0180005012.01.T01:CDS | 30.0% | |
| GCACCAAAGTTAAGAAAATT+CGG | + | Chr1:85654893-85654912 | None:intergenic | 30.0% | |
| TAAGTTATGGAATGAAGATG+GGG | - | Chr1:85655915-85655934 | MsG0180005012.01.T01:CDS | 30.0% | |
| TCTACCTCTTCTATAACATT+TGG | + | Chr1:85655178-85655197 | None:intergenic | 30.0% | |
| TTCAATAAACTTCATGCCAA+TGG | - | Chr1:85655223-85655242 | MsG0180005012.01.T01:intron | 30.0% | |
| ! | ATGCCGAATTTTCTTAACTT+TGG | - | Chr1:85654887-85654906 | MsG0180005012.01.T01:CDS | 30.0% |
| ! | TTACAGACCTTGAGAAATTT+TGG | - | Chr1:85655755-85655774 | MsG0180005012.01.T01:CDS | 30.0% |
| !! | ACTTGTTGCTTTCTAATGAA+AGG | - | Chr1:85655868-85655887 | MsG0180005012.01.T01:CDS | 30.0% |
| !! | CTTGTTGCTTTCTAATGAAA+GGG | - | Chr1:85655869-85655888 | MsG0180005012.01.T01:CDS | 30.0% |
| !!! | AACGTTTTGAGAGTATTGAT+GGG | + | Chr1:85654950-85654969 | None:intergenic | 30.0% |
| !!! | AATGTGAGTTTTTCAAGCAA+CGG | + | Chr1:85655563-85655582 | None:intergenic | 30.0% |
| CAGTATTTGACATAGTCCAT+TGG | + | Chr1:85655242-85655261 | None:intergenic | 35.0% | |
| GTTGCTTGAAAAACTCACAT+TGG | - | Chr1:85655562-85655581 | MsG0180005012.01.T01:intron | 35.0% | |
| TCTAATGAAAGGGAAAGCTT+TGG | - | Chr1:85655879-85655898 | MsG0180005012.01.T01:CDS | 35.0% | |
| TTCTCAAGGTCTGTAAGATA+GGG | + | Chr1:85655751-85655770 | None:intergenic | 35.0% | |
| TTGAGAATTTGAGTCTGAAG+AGG | - | Chr1:85654990-85655009 | MsG0180005012.01.T01:CDS | 35.0% | |
| TTTCTCAAGGTCTGTAAGAT+AGG | + | Chr1:85655752-85655771 | None:intergenic | 35.0% | |
| ! | TTTTTGTACTCACTGTCACT+AGG | + | Chr1:85655454-85655473 | None:intergenic | 35.0% |
| !!! | ATGTGAGTTTTTCAAGCAAC+GGG | + | Chr1:85655562-85655581 | None:intergenic | 35.0% |
| !!! | CAACGTTTTGAGAGTATTGA+TGG | + | Chr1:85654951-85654970 | None:intergenic | 35.0% |
| AGCAACAAGTCCTAAGTTCT+TGG | + | Chr1:85655858-85655877 | None:intergenic | 40.0% | |
| CTTCAATCCAACCCAAAGAA+AGG | + | Chr1:85654925-85654944 | None:intergenic | 40.0% | |
| GCACTCAAAGACCTTTCTTT+GGG | - | Chr1:85654911-85654930 | MsG0180005012.01.T01:CDS | 40.0% | |
| GCGTTTGCCAAAATTTCTCA+AGG | + | Chr1:85655765-85655784 | None:intergenic | 40.0% | |
| TCAAAGACCTTTCTTTGGGT+TGG | - | Chr1:85654915-85654934 | MsG0180005012.01.T01:CDS | 40.0% | |
| TGCACTCAAAGACCTTTCTT+TGG | - | Chr1:85654910-85654929 | MsG0180005012.01.T01:CDS | 40.0% | |
| ! | ATTTGAGTCTGAAGAGGTGT+TGG | - | Chr1:85654996-85655015 | MsG0180005012.01.T01:CDS | 40.0% |
| !! | TTTGAGTCTGAAGAGGTGTT+GGG | - | Chr1:85654997-85655016 | MsG0180005012.01.T01:CDS | 40.0% |
| AAAAAGGTTGCTCCATCTGC+AGG | - | Chr1:85655425-85655444 | MsG0180005012.01.T01:CDS | 45.0% | |
| TGCAAATTCTCTGAACGCTC+TGG | + | Chr1:85655985-85656004 | None:intergenic | 45.0% | |
| ! | AGTCCTAAGTTCTTGGCTTG+TGG | + | Chr1:85655851-85655870 | None:intergenic | 45.0% |
| !! | TCTGAAGAGGTGTTGGGATT+TGG | - | Chr1:85655003-85655022 | MsG0180005012.01.T01:CDS | 45.0% |
| GAGCCACAAGCCAAGAACTT+AGG | - | Chr1:85655845-85655864 | MsG0180005012.01.T01:CDS | 50.0% | |
| GGTTTGCAGCTATCTACTCC+AGG | - | Chr1:85655291-85655310 | MsG0180005012.01.T01:intron | 50.0% | |
| GTAAACGTTGTGCACGTCGA+CGG | + | Chr1:85655954-85655973 | None:intergenic | 50.0% | |
| !! | GAAGAGGTGTTGGGATTTGG+TGG | - | Chr1:85655006-85655025 | MsG0180005012.01.T01:CDS | 50.0% |
| GTCACTAGGTCACCTGCAGA+TGG | + | Chr1:85655440-85655459 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 85654887 | 85656020 | 85654887 | ID=MsG0180005012.01;Name=MsG0180005012.01 |
| Chr1 | mRNA | 85654887 | 85656020 | 85654887 | ID=MsG0180005012.01.T01;Parent=MsG0180005012.01;Name=MsG0180005012.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|0.66|3|0|289 |
| Chr1 | exon | 85655616 | 85656020 | 85655616 | ID=MsG0180005012.01.T01:exon:9705;Parent=MsG0180005012.01.T01 |
| Chr1 | exon | 85655297 | 85655476 | 85655297 | ID=MsG0180005012.01.T01:exon:9704;Parent=MsG0180005012.01.T01 |
| Chr1 | exon | 85654887 | 85655171 | 85654887 | ID=MsG0180005012.01.T01:exon:9703;Parent=MsG0180005012.01.T01 |
| Chr1 | CDS | 85655616 | 85656020 | 85655616 | ID=MsG0180005012.01.T01:cds;Parent=MsG0180005012.01.T01 |
| Chr1 | CDS | 85655297 | 85655476 | 85655297 | ID=MsG0180005012.01.T01:cds;Parent=MsG0180005012.01.T01 |
| Chr1 | CDS | 85654887 | 85655171 | 85654887 | ID=MsG0180005012.01.T01:cds;Parent=MsG0180005012.01.T01 |
| Gene Sequence |
| Protein sequence |
