AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043692.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043692.01.T01 MTR_8g039290 98.561 139 2 0 1 139 84 222 5.94e-98 286
MsG0880043692.01.T01 MTR_8g039290 98.561 139 2 0 1 139 84 222 6.48e-98 286
MsG0880043692.01.T01 MTR_5g089520 79.137 139 29 0 1 139 72 210 1.41e-79 239
MsG0880043692.01.T01 MTR_1g012920 69.595 148 42 2 1 146 84 230 1.98e-70 216
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043692.01.T01 AT5G13500 80.137 146 28 1 1 146 82 226 1.06e-81 245
MsG0880043692.01.T01 AT5G13500 80.137 146 28 1 1 146 82 226 1.06e-81 245
MsG0880043692.01.T01 AT5G13500 80.137 146 28 1 1 146 82 226 1.06e-81 245
MsG0880043692.01.T01 AT2G25260 70.504 139 41 0 1 139 83 221 3.29e-72 221
MsG0880043692.01.T01 AT5G25265 68.919 148 43 2 1 146 89 235 5.33e-69 213

Find 36 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTTCTATTAATTGAAT+TGG 0.254602 8:+29280201 MsG0880043692.01.T01:CDS
TCTACCTTTCTGATAATTAC+AGG 0.273833 8:-29280169 None:intergenic
TGTACGCCCTCTTCCCAATC+TGG 0.371070 8:+29280018 MsG0880043692.01.T01:CDS
CCTCTTCCCAATCTGGCATT+TGG 0.385015 8:+29280025 MsG0880043692.01.T01:CDS
CTCACCCTGTCTAGACCTTC+CGG 0.417765 8:-29278887 None:intergenic
TGGAAAGGCTGACAACTTGA+TGG 0.418539 8:+29278832 MsG0880043692.01.T01:CDS
TGTCGTCGTTGATCCTCTTC+CGG 0.440206 8:+29278868 MsG0880043692.01.T01:CDS
CTTTGTTCAGTGGCTGGAAA+AGG 0.444264 8:+29279866 MsG0880043692.01.T01:CDS
ATGGGCCTTTGTTCAGTGGC+TGG 0.460296 8:+29279860 MsG0880043692.01.T01:CDS
GGGCGTACAAATACGTGATC+AGG 0.464327 8:-29280004 None:intergenic
ATCGGATCAATATTTGTAAC+CGG 0.473029 8:-29280136 None:intergenic
CTCTTCCGGAAGGTCTAGAC+AGG 0.482298 8:+29278882 MsG0880043692.01.T01:CDS
CCAAATGCCAGATTGGGAAG+AGG 0.493455 8:-29280025 None:intergenic
CTGTCTAGACCTTCCGGAAG+AGG 0.500411 8:-29278881 None:intergenic
GTAACCGGTCCATTCTCCTC+AGG 0.508925 8:-29280121 None:intergenic
AAGTATTATCCTGAGGAGAA+TGG 0.515266 8:+29280112 MsG0880043692.01.T01:CDS
AATCCTCCCATCTCTGATCC+AGG 0.523340 8:-29278791 None:intergenic
GAGTCTACCTGGATCAGAGA+TGG 0.533196 8:+29278784 MsG0880043692.01.T01:CDS
TTCTCCTGTAATTATCAGAA+AGG 0.533563 8:+29280165 MsG0880043692.01.T01:CDS
AGAGGATCAACGACGACAGT+AGG 0.536647 8:-29278863 None:intergenic
AATGCCGTATCATGTATTAC+TGG 0.539444 8:+29278747 None:intergenic
AAGAAGCAAAGGAGTCTACC+TGG 0.540779 8:+29278773 MsG0880043692.01.T01:CDS
GACCATGGGCCTTTGTTCAG+TGG 0.540787 8:+29279856 MsG0880043692.01.T01:CDS
GTTACAAATATTGATCCGAT+TGG 0.548705 8:+29280139 MsG0880043692.01.T01:CDS
GTCGTTGATCCTCTTCCGGA+AGG 0.555563 8:+29278872 MsG0880043692.01.T01:CDS
CAAATGCCAGATTGGGAAGA+GGG 0.557171 8:-29280024 None:intergenic
ATTACAGGAGAATTGCCAAT+CGG 0.588392 8:-29280154 None:intergenic
AGTCTACCTGGATCAGAGAT+GGG 0.590857 8:+29278785 MsG0880043692.01.T01:CDS
ATTACTGGTATAAGAAGCAA+AGG 0.598311 8:+29278762 MsG0880043692.01.T01:CDS
TTATCCTGAGGAGAATGGAC+CGG 0.623990 8:+29280117 MsG0880043692.01.T01:CDS
CGTCGTTCTGAATAGACCAT+GGG 0.624386 8:+29279842 MsG0880043692.01.T01:CDS
AGCCACTGAACAAAGGCCCA+TGG 0.626962 8:-29279858 None:intergenic
ACGTCGTTCTGAATAGACCA+TGG 0.640320 8:+29279841 MsG0880043692.01.T01:CDS
CTACCTGGATCAGAGATGGG+AGG 0.675794 8:+29278788 MsG0880043692.01.T01:CDS
TGTAAGGAAGTATTATCCTG+AGG 0.689724 8:+29280105 MsG0880043692.01.T01:CDS
TCTTCCGGAAGGTCTAGACA+GGG 0.732437 8:+29278883 MsG0880043692.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAAAATGTATTTGTTT+AGG + Chr8:29279626-29279645 MsG0880043692.01.T01:intron 10.0%
!!! AAATACATTTTATAAATGAT+TGG - Chr8:29279622-29279641 None:intergenic 10.0%
!!! TTTATTTTCAAATGTTAAAA+TGG - Chr8:29279307-29279326 None:intergenic 10.0%
!!! TTTTAACATTTGAAAATAAA+AGG + Chr8:29279307-29279326 MsG0880043692.01.T01:intron 10.0%
!! AAGAAAATGAAAAAATTGTA+AGG + Chr8:29280089-29280108 MsG0880043692.01.T01:CDS 15.0%
!! ATTTAAATTGTATTTACTGA+TGG - Chr8:29279788-29279807 None:intergenic 15.0%
!! TAAATCAATCTATCAATAAT+AGG - Chr8:29279755-29279774 None:intergenic 15.0%
!!! AAATATTTTGTGTATCAATT+TGG + Chr8:29279592-29279611 MsG0880043692.01.T01:intron 15.0%
!!! ACAATTTTTTCATTTTCTTT+AGG - Chr8:29280088-29280107 None:intergenic 15.0%
!! AGTTTATCAAAATAGCTTAT+GGG + Chr8:29279080-29279099 MsG0880043692.01.T01:intron 20.0%
!! ATACTATTAATTCAATAAGC+TGG - Chr8:29278976-29278995 None:intergenic 20.0%
!! ATTTGAAAATAAAAGGAATG+CGG + Chr8:29279314-29279333 MsG0880043692.01.T01:intron 20.0%
!! GAAACTTCTATTAATTGAAT+TGG + Chr8:29280201-29280220 MsG0880043692.01.T01:CDS 20.0%
!! TAAAATCCGACAAAATAATT+TGG + Chr8:29279367-29279386 MsG0880043692.01.T01:intron 20.0%
!!! ACAATACCAAATTATTTTGT+CGG - Chr8:29279376-29279395 None:intergenic 20.0%
!!! TTGATGCAGATATATTTTAA+TGG + Chr8:29279976-29279995 MsG0880043692.01.T01:intron 20.0%
!!! TTGTAGTTTGTTGTTATAAT+TGG + Chr8:29278995-29279014 MsG0880043692.01.T01:intron 20.0%
! AACTAAAACATAGATCACTT+AGG - Chr8:29279922-29279941 None:intergenic 25.0%
! AGATAGAAATAGTATAAGCT+CGG + Chr8:29279535-29279554 MsG0880043692.01.T01:intron 25.0%
! GAGTTTATCAAAATAGCTTA+TGG + Chr8:29279079-29279098 MsG0880043692.01.T01:intron 25.0%
! GGTATTGTTGAAATAATTCA+AGG + Chr8:29279388-29279407 MsG0880043692.01.T01:intron 25.0%
! TATTGCAAAGTTTAGTGTTA+AGG + Chr8:29279558-29279577 MsG0880043692.01.T01:intron 25.0%
! TTAGGCTTAATGTAGAAAAA+TGG - Chr8:29280070-29280089 None:intergenic 25.0%
!! AAAAGTATAACTGAAAGTCA+TGG - Chr8:29278921-29278940 None:intergenic 25.0%
!! ATAAGCACTTTTATGATAAG+TGG + Chr8:29279210-29279229 MsG0880043692.01.T01:intron 25.0%
!! ATGTTAAAATGGACTTTAAC+TGG - Chr8:29279296-29279315 None:intergenic 25.0%
!! TTCACTAGAATTTTACATTC+TGG + Chr8:29278812-29278831 MsG0880043692.01.T01:CDS 25.0%
!! TTGTTATAATTGGTCTGTTT+GGG + Chr8:29279005-29279024 MsG0880043692.01.T01:intron 25.0%
!!! TAGAATTTTACATTCTGGAA+AGG + Chr8:29278817-29278836 MsG0880043692.01.T01:CDS 25.0%
AGTCGTGTGCTTAAATTTAA+AGG + Chr8:29279466-29279485 MsG0880043692.01.T01:intron 30.0%
ATAATAGGAGAGTCTAAACA+TGG - Chr8:29279740-29279759 None:intergenic 30.0%
ATCGGATCAATATTTGTAAC+CGG - Chr8:29280139-29280158 None:intergenic 30.0%
ATGATATCAGATTGAGGAAA+GGG + Chr8:29279411-29279430 MsG0880043692.01.T01:intron 30.0%
ATTACTGGTATAAGAAGCAA+AGG + Chr8:29278762-29278781 MsG0880043692.01.T01:CDS 30.0%
GTTACAAATATTGATCCGAT+TGG + Chr8:29280139-29280158 MsG0880043692.01.T01:CDS 30.0%
TCTACCTTTCTGATAATTAC+AGG - Chr8:29280172-29280191 None:intergenic 30.0%
TTATCCGGAAAATCTATTGT+AGG + Chr8:29279687-29279706 MsG0880043692.01.T01:intron 30.0%
TTCTATCTTCGACAAACAAA+AGG - Chr8:29279523-29279542 None:intergenic 30.0%
TTCTCCTGTAATTATCAGAA+AGG + Chr8:29280165-29280184 MsG0880043692.01.T01:CDS 30.0%
TTTAGGAAGTTACTGTGATA+AGG + Chr8:29279643-29279662 MsG0880043692.01.T01:intron 30.0%
! GTTGTTATAATTGGTCTGTT+TGG + Chr8:29279004-29279023 MsG0880043692.01.T01:intron 30.0%
! GTTTCCTACAATAGATTTTC+CGG - Chr8:29279694-29279713 None:intergenic 30.0%
AAGTATTATCCTGAGGAGAA+TGG + Chr8:29280112-29280131 MsG0880043692.01.T01:CDS 35.0%
ACACATTGCTTAATCTTTGC+AGG + Chr8:29279815-29279834 MsG0880043692.01.T01:intron 35.0%
AGTTTAGTGTTAAGGTTTGC+AGG + Chr8:29279566-29279585 MsG0880043692.01.T01:intron 35.0%
ATTACAGGAGAATTGCCAAT+CGG - Chr8:29280157-29280176 None:intergenic 35.0%
CACATTGCTTAATCTTTGCA+GGG + Chr8:29279816-29279835 MsG0880043692.01.T01:intron 35.0%
GATGATATCAGATTGAGGAA+AGG + Chr8:29279410-29279429 MsG0880043692.01.T01:intron 35.0%
TAACTGAAAGTCATGGTAAG+CGG - Chr8:29278914-29278933 None:intergenic 35.0%
TAGAAAAATGGAAAAGCAGC+TGG - Chr8:29280058-29280077 None:intergenic 35.0%
TCAAGGATGATATCAGATTG+AGG + Chr8:29279405-29279424 MsG0880043692.01.T01:intron 35.0%
! AAAATGGACTTTAACTGGCA+TGG - Chr8:29279291-29279310 None:intergenic 35.0%
! ATTTTCTCCAAATGCCAGAT+TGG - Chr8:29280035-29280054 None:intergenic 35.0%
! GGATTTTACTTCTTTCACCA+AGG - Chr8:29279355-29279374 None:intergenic 35.0%
! TATAATTGGTCTGTTTGGGT+TGG + Chr8:29279009-29279028 MsG0880043692.01.T01:intron 35.0%
! TTTTCTCCAAATGCCAGATT+GGG - Chr8:29280034-29280053 None:intergenic 35.0%
!! TGTAAGGAAGTATTATCCTG+AGG + Chr8:29280105-29280124 MsG0880043692.01.T01:CDS 35.0%
ACTTAGCTGTTTGTCCAAAC+AGG + Chr8:29279246-29279265 MsG0880043692.01.T01:intron 40.0%
GTCCACAAAGACACATTATC+CGG + Chr8:29279672-29279691 MsG0880043692.01.T01:intron 40.0%
TTCCGGATAATGTGTCTTTG+TGG - Chr8:29279677-29279696 None:intergenic 40.0%
TTCTAGACTAGGATCCTGTT+TGG - Chr8:29279263-29279282 None:intergenic 40.0%
!! GCATGGAAGTATTCTAGACT+AGG - Chr8:29279274-29279293 None:intergenic 40.0%
AAGAAGCAAAGGAGTCTACC+TGG + Chr8:29278773-29278792 MsG0880043692.01.T01:CDS 45.0%
AGTCTACCTGGATCAGAGAT+GGG + Chr8:29278785-29278804 MsG0880043692.01.T01:CDS 45.0%
CAAATGCCAGATTGGGAAGA+GGG - Chr8:29280027-29280046 None:intergenic 45.0%
CTTTGTTCAGTGGCTGGAAA+AGG + Chr8:29279866-29279885 MsG0880043692.01.T01:CDS 45.0%
TGGAAAGGCTGACAACTTGA+TGG + Chr8:29278832-29278851 MsG0880043692.01.T01:CDS 45.0%
TTATCCTGAGGAGAATGGAC+CGG + Chr8:29280117-29280136 MsG0880043692.01.T01:CDS 45.0%
! ACGTCGTTCTGAATAGACCA+TGG + Chr8:29279841-29279860 MsG0880043692.01.T01:CDS 45.0%
! CGTCGTTCTGAATAGACCAT+GGG + Chr8:29279842-29279861 MsG0880043692.01.T01:CDS 45.0%
! CTTTTCCAGCCACTGAACAA+AGG - Chr8:29279868-29279887 None:intergenic 45.0%
! GGTTTTCGACTAGCATACCT+TGG + Chr8:29279335-29279354 MsG0880043692.01.T01:intron 45.0%
AATCCTCCCATCTCTGATCC+AGG - Chr8:29278794-29278813 None:intergenic 50.0%
AGAGGATCAACGACGACAGT+AGG - Chr8:29278866-29278885 None:intergenic 50.0%
CCAAATGCCAGATTGGGAAG+AGG - Chr8:29280028-29280047 None:intergenic 50.0%
CCTCTTCCCAATCTGGCATT+TGG + Chr8:29280025-29280044 MsG0880043692.01.T01:CDS 50.0%
GAGTCTACCTGGATCAGAGA+TGG + Chr8:29278784-29278803 MsG0880043692.01.T01:CDS 50.0%
GGGCGTACAAATACGTGATC+AGG - Chr8:29280007-29280026 None:intergenic 50.0%
TCTTCCGGAAGGTCTAGACA+GGG + Chr8:29278883-29278902 MsG0880043692.01.T01:CDS 50.0%
TGTCGTCGTTGATCCTCTTC+CGG + Chr8:29278868-29278887 MsG0880043692.01.T01:CDS 50.0%
AGCCACTGAACAAAGGCCCA+TGG - Chr8:29279861-29279880 None:intergenic 55.0%
ATGGGCCTTTGTTCAGTGGC+TGG + Chr8:29279860-29279879 MsG0880043692.01.T01:CDS 55.0%
CTACCTGGATCAGAGATGGG+AGG + Chr8:29278788-29278807 MsG0880043692.01.T01:CDS 55.0%
CTCACCCTGTCTAGACCTTC+CGG - Chr8:29278890-29278909 None:intergenic 55.0%
CTCTTCCGGAAGGTCTAGAC+AGG + Chr8:29278882-29278901 MsG0880043692.01.T01:CDS 55.0%
CTGTCTAGACCTTCCGGAAG+AGG - Chr8:29278884-29278903 None:intergenic 55.0%
GACCATGGGCCTTTGTTCAG+TGG + Chr8:29279856-29279875 MsG0880043692.01.T01:CDS 55.0%
GTAACCGGTCCATTCTCCTC+AGG - Chr8:29280124-29280143 None:intergenic 55.0%
GTCGTTGATCCTCTTCCGGA+AGG + Chr8:29278872-29278891 MsG0880043692.01.T01:CDS 55.0%
TGTACGCCCTCTTCCCAATC+TGG + Chr8:29280018-29280037 MsG0880043692.01.T01:CDS 55.0%
CGGAAGGTCTAGACAGGGTG+AGG + Chr8:29278888-29278907 MsG0880043692.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 29278758 29280228 29278758 ID=MsG0880043692.01;Name=MsG0880043692.01
Chr8 mRNA 29278758 29280228 29278758 ID=MsG0880043692.01.T01;Parent=MsG0880043692.01;Name=MsG0880043692.01.T01;_AED=0.46;_eAED=0.47;_QI=0|0|0|1|1|0.66|3|0|152
Chr8 exon 29278758 29278904 29278758 ID=MsG0880043692.01.T01:exon:6799;Parent=MsG0880043692.01.T01
Chr8 exon 29279837 29279904 29279837 ID=MsG0880043692.01.T01:exon:6800;Parent=MsG0880043692.01.T01
Chr8 exon 29279985 29280228 29279985 ID=MsG0880043692.01.T01:exon:6801;Parent=MsG0880043692.01.T01
Chr8 CDS 29278758 29278904 29278758 ID=MsG0880043692.01.T01:cds;Parent=MsG0880043692.01.T01
Chr8 CDS 29279837 29279904 29279837 ID=MsG0880043692.01.T01:cds;Parent=MsG0880043692.01.T01
Chr8 CDS 29279985 29280228 29279985 ID=MsG0880043692.01.T01:cds;Parent=MsG0880043692.01.T01
Gene Sequence

>MsG0880043692.01.T01

ATGTATTACTGGTATAAGAAGCAAAGGAGTCTACCTGGATCAGAGATGGGAGGATTCACTAGAATTTTACATTCTGGAAAGGCTGACAACTTGATGGATGAAATTCCTACTGTCGTCGTTGATCCTCTTCCGGAAGGTCTAGACAGGGGATACGTCGTTCTGAATAGACCATGGGCCTTTGTTCAGTGGCTGGAAAAGGCTAAAATAGAAGAAGAATATATTTTAATGGCAGAACCTGATCACGTATTTGTACGCCCTCTTCCCAATCTGGCATTTGGAGAAAATCCAGCTGCTTTTCCATTTTTCTACATTAAGCCTAAAGAAAATGAAAAAATTGTAAGGAAGTATTATCCTGAGGAGAATGGACCGGTTACAAATATTGATCCGATTGGCAATTCTCCTGTAATTATCAGAAAGGTAGAATCTCTTCAGAAACTTCTATTAATTGAATTGGATTAA

Protein sequence

>MsG0880043692.01.T01

MYYWYKKQRSLPGSEMGGFTRILHSGKADNLMDEIPTVVVDPLPEGLDRGYVVLNRPWAFVQWLEKAKIEEEYILMAEPDHVFVRPLPNLAFGENPAAFPFFYIKPKENEKIVRKYYPEENGPVTNIDPIGNSPVIIRKVESLQKLLLIELD*