AlfalfaGEDB | MsG0280007876.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007876.01

KEGG: K07953
GO: GO:0005525 GO:0005622 GO:0006886
PFAM: PF00025

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007876.01.T01	GAU48265.1	98.305	118	2	0	1	118	1	118	9.40E-81	242

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007876.01.T01	O04834	95.327	107	5	0	12	118	87	193	1.24E-71	214

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007876.01.T01	A0A2Z6PF65	98.305	118	2	0	1	118	1	118	4.49e-81	242

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0280007876.01	0.899799	1.385529e-77	1.739915e-73
MsG0080048058.01	MsG0280007876.01	0.802759	4.816961e-49	2.379001e-46
MsG0080048383.01	MsG0280007876.01	0.811770	5.927804e-51	3.701644e-48
MsG0080048687.01	MsG0280007876.01	0.825811	3.824420e-54	3.523334e-51
MsG0080049059.01	MsG0280007876.01	0.817005	4.126659e-52	2.969990e-49
MsG0180000001.01	MsG0280007876.01	0.874976	4.431912e-68	2.035341e-64
MsG0180000135.01	MsG0280007876.01	0.811176	7.980534e-51	4.905892e-48
MsG0180000197.01	MsG0280007876.01	0.803980	2.690383e-49	1.371015e-46
MsG0180000364.01	MsG0280007876.01	0.823493	1.344390e-53	1.159411e-50
MsG0180000446.01	MsG0280007876.01	0.805328	1.407457e-49	7.425295e-47
MsG0180000799.01	MsG0280007876.01	0.832917	7.190804e-56	8.152673e-53
MsG0180001117.01	MsG0280007876.01	0.847729	9.617127e-60	1.725340e-56
MsG0180001237.01	MsG0280007876.01	0.849516	3.075064e-60	5.843878e-57
MsG0180001260.01	MsG0280007876.01	0.804942	1.695751e-49	8.858257e-47
MsG0180001294.01	MsG0280007876.01	0.842060	3.255511e-58	4.877502e-55
MsG0180001566.01	MsG0280007876.01	0.812287	4.574524e-51	2.896850e-48
MsG0180001599.01	MsG0280007876.01	0.809308	2.017526e-50	1.180438e-47
MsG0180002491.01	MsG0280007876.01	0.804107	2.532212e-49	1.294552e-46
MsG0180003337.01	MsG0280007876.01	0.833562	4.967675e-56	5.742086e-53
MsG0180003444.01	MsG0280007876.01	0.807306	5.392107e-50	2.993082e-47
MsG0180003744.01	MsG0280007876.01	0.819637	1.046141e-52	8.094978e-50
MsG0180003860.01	MsG0280007876.01	0.843941	1.028073e-58	1.634236e-55
MsG0180003984.01	MsG0280007876.01	0.820629	6.199027e-53	4.930544e-50
MsG0180004050.01	MsG0280007876.01	0.811749	5.991740e-51	3.739468e-48
MsG0180004598.01	MsG0280007876.01	0.805576	1.248592e-49	6.629128e-47
MsG0180004754.01	MsG0280007876.01	0.805974	1.030250e-49	5.526233e-47
MsG0180004767.01	MsG0280007876.01	0.846232	2.472284e-59	4.226626e-56
MsG0180004989.01	MsG0280007876.01	0.844011	9.842801e-59	1.568018e-55
MsG0180004991.01	MsG0280007876.01	0.879270	1.429395e-69	7.703132e-66
MsG0180005125.01	MsG0280007876.01	0.817744	2.813316e-52	2.066110e-49
MsG0180005324.01	MsG0280007876.01	0.806930	6.474690e-50	3.559021e-47
MsG0180005337.01	MsG0280007876.01	0.822978	1.773363e-53	1.507283e-50
MsG0180005765.01	MsG0280007876.01	0.836234	1.054450e-56	1.320936e-53
MsG0180006013.01	MsG0280007876.01	0.815472	9.084061e-52	6.269448e-49
MsG0180006235.01	MsG0280007876.01	0.855652	5.462936e-62	1.270276e-58
MsG0280006305.01	MsG0280007876.01	0.831851	1.320341e-55	1.450646e-52
MsG0280006759.01	MsG0280007876.01	0.869379	3.235696e-66	1.211904e-62
MsG0280007024.01	MsG0280007876.01	0.803760	2.989058e-49	1.514573e-46
MsG0280007071.01	MsG0280007876.01	0.824779	6.707639e-54	6.000531e-51
MsG0280007089.01	MsG0280007876.01	0.820361	7.143205e-53	5.639935e-50
MsG0280007191.01	MsG0280007876.01	0.807420	5.098693e-50	2.838614e-47
MsG0280007196.01	MsG0280007876.01	0.808619	2.833121e-50	1.627474e-47
MsG0280007440.01	MsG0280007876.01	0.812430	4.255889e-51	2.705569e-48
MsG0280007609.01	MsG0280007876.01	0.858610	7.322264e-63	1.879565e-59
MsG0280007790.01	MsG0280007876.01	0.881204	2.914216e-70	1.691871e-66
MsG0280007876.01	MsG0280007983.01	0.821975	3.034242e-53	2.506158e-50
MsG0280007876.01	MsG0280008134.01	0.824709	6.969399e-54	6.222390e-51
MsG0280007876.01	MsG0280008286.01	0.824532	7.668788e-54	6.811383e-51
MsG0280007876.01	MsG0280008327.01	0.863765	1.971286e-64	6.045984e-61
MsG0280007876.01	MsG0280008885.01	0.837240	5.840125e-57	7.539671e-54
MsG0280007876.01	MsG0280008909.01	0.811395	7.152852e-51	4.422664e-48
MsG0280007876.01	MsG0280009111.01	0.807775	4.286516e-50	2.408521e-47
MsG0280007876.01	MsG0280009514.01	0.808147	3.572750e-50	2.027283e-47
MsG0280007876.01	MsG0280010002.01	0.879583	1.106631e-69	6.032930e-66
MsG0280007876.01	MsG0280010389.01	0.807651	4.555575e-50	2.551371e-47
MsG0280007876.01	MsG0280010633.01	0.867625	1.192411e-65	4.190837e-62
MsG0280007876.01	MsG0280010794.01	0.836403	9.553569e-57	1.202654e-53
MsG0280007876.01	MsG0280011037.01	0.820415	6.941796e-53	5.489589e-50
MsG0280007876.01	MsG0280011332.01	0.853249	2.706686e-61	5.811525e-58
MsG0280007876.01	MsG0380014489.01	0.812284	4.580320e-51	2.900372e-48
MsG0280007876.01	MsG0380015512.01	0.819477	1.138079e-52	8.767625e-50
MsG0280007876.01	MsG0380015630.01	0.807419	5.101830e-50	2.840249e-47
MsG0280007876.01	MsG0380015793.01	-0.800923	1.147771e-48	5.410609e-46
MsG0280007876.01	MsG0380015831.01	0.832327	1.006929e-55	1.121650e-52
MsG0280007876.01	MsG0380016389.01	0.864877	8.863969e-65	2.825790e-61
MsG0280007876.01	MsG0380016483.01	0.815870	7.408822e-52	5.168592e-49
MsG0280007876.01	MsG0380016513.01	0.809163	2.167043e-50	1.263060e-47
MsG0280007876.01	MsG0380016882.01	0.821587	3.730763e-53	3.047649e-50
MsG0280007876.01	MsG0380016971.01	0.833651	4.718726e-56	5.468722e-53
MsG0280007876.01	MsG0380016985.01	0.836646	8.282733e-57	1.050212e-53
MsG0280007876.01	MsG0380017049.01	0.814534	1.467377e-51	9.871686e-49
MsG0280007876.01	MsG0380017195.01	0.811618	6.397980e-51	3.979027e-48
MsG0280007876.01	MsG0380017240.01	0.802691	4.975193e-49	2.452830e-46
MsG0280007876.01	MsG0380017330.01	0.809983	1.444623e-50	8.605173e-48
MsG0280007876.01	MsG0380017375.01	0.805156	1.529591e-49	8.033931e-47
MsG0280007876.01	MsG0380017431.01	0.830335	3.112006e-55	3.269069e-52
MsG0280007876.01	MsG0380017604.01	0.801926	7.148556e-49	3.456341e-46
MsG0280007876.01	MsG0380017643.01	0.817129	3.869357e-52	2.794098e-49
MsG0280007876.01	MsG0380017719.01	0.825863	3.717462e-54	3.429919e-51
MsG0280007876.01	MsG0380017881.01	0.810516	1.108823e-50	6.697533e-48
MsG0280007876.01	MsG0480018126.01	0.808154	3.559533e-50	2.020195e-47
MsG0280007876.01	MsG0480018256.01	0.825529	4.461298e-54	4.076786e-51
MsG0280007876.01	MsG0480018462.01	0.845606	3.657671e-59	6.128187e-56
MsG0280007876.01	MsG0480018477.01	0.803415	3.524207e-49	1.769939e-46
MsG0280007876.01	MsG0480018641.01	0.849132	3.933974e-60	7.385707e-57
MsG0280007876.01	MsG0480018846.01	0.817690	2.893586e-52	2.121838e-49
MsG0280007876.01	MsG0480019170.01	0.801516	8.679071e-49	4.152983e-46
MsG0280007876.01	MsG0480020242.01	0.814304	1.649575e-51	1.102889e-48
MsG0280007876.01	MsG0480020331.01	0.821719	3.477174e-53	2.851341e-50
MsG0280007876.01	MsG0480020372.01	0.804122	2.513945e-49	1.285716e-46
MsG0280007876.01	MsG0480020591.01	0.813065	3.090295e-51	1.997672e-48
MsG0280007876.01	MsG0480020791.01	0.815302	9.911823e-52	6.808024e-49
MsG0280007876.01	MsG0480020917.01	0.876044	1.908508e-68	9.117113e-65
MsG0280007876.01	MsG0480021225.01	0.813554	2.413540e-51	1.581144e-48
MsG0280007876.01	MsG0480021772.01	0.819540	1.100837e-52	8.495561e-50
MsG0280007876.01	MsG0480021812.01	0.834249	3.342737e-56	3.944621e-53
MsG0280007876.01	MsG0480021832.01	0.830156	3.441710e-55	3.596734e-52
MsG0280007876.01	MsG0480021914.01	0.800055	1.724570e-48	7.952335e-46
MsG0280007876.01	MsG0480022167.01	0.870147	1.816872e-66	6.993831e-63
MsG0280007876.01	MsG0480022183.01	0.800263	1.564640e-48	7.253077e-46
MsG0280007876.01	MsG0480022228.01	0.801423	9.069244e-49	4.329413e-46
MsG0280007876.01	MsG0480022464.01	0.804903	1.727918e-49	9.017545e-47
MsG0280007876.01	MsG0480022480.01	0.807641	4.578075e-50	2.563301e-47
MsG0280007876.01	MsG0480022620.01	0.813219	2.859207e-51	1.855978e-48
MsG0280007876.01	MsG0480022678.01	0.819973	8.764797e-53	6.846571e-50
MsG0280007876.01	MsG0480022717.01	0.854539	1.150771e-61	2.578691e-58
MsG0280007876.01	MsG0480022719.01	0.817612	3.013323e-52	2.204895e-49
MsG0280007876.01	MsG0480023013.01	0.841221	5.420474e-58	7.910228e-55
MsG0280007876.01	MsG0480023245.01	0.813821	2.108302e-51	1.391166e-48
MsG0280007876.01	MsG0480023258.01	0.813909	2.016063e-51	1.333447e-48
MsG0280007876.01	MsG0480023361.01	0.806445	8.196339e-50	4.449593e-47
MsG0280007876.01	MsG0480023412.01	0.814866	1.238504e-51	8.406442e-49
MsG0280007876.01	MsG0480023447.01	0.838609	2.597175e-57	3.497067e-54
MsG0280007876.01	MsG0480023500.01	0.827288	1.699340e-54	1.633474e-51
MsG0280007876.01	MsG0480023559.01	0.847502	1.110211e-59	1.977410e-56
MsG0280007876.01	MsG0480023737.01	0.802749	4.840085e-49	2.389814e-46
MsG0280007876.01	MsG0580024478.01	0.815540	8.774042e-52	6.066282e-49
MsG0280007876.01	MsG0580024618.01	0.851486	8.601161e-61	1.743497e-57
MsG0280007876.01	MsG0580024792.01	0.814639	1.390355e-51	9.380179e-49
MsG0280007876.01	MsG0580024819.01	0.817667	2.927855e-52	2.145646e-49
MsG0280007876.01	MsG0580025134.01	0.804370	2.232013e-49	1.148832e-46
MsG0280007876.01	MsG0580025352.01	0.822724	2.032614e-53	1.715226e-50
MsG0280007876.01	MsG0580025408.01	0.801779	7.664429e-49	3.691887e-46
MsG0280007876.01	MsG0580025989.01	0.815434	9.262841e-52	6.385594e-49
MsG0280007876.01	MsG0580026157.01	0.865322	6.420904e-65	2.080257e-61
MsG0280007876.01	MsG0580027413.01	0.808194	3.491269e-50	1.983532e-47
MsG0280007876.01	MsG0580027441.01	0.811461	6.919325e-51	4.285792e-48
MsG0280007876.01	MsG0580028060.01	0.836177	1.089999e-56	1.363121e-53
MsG0280007876.01	MsG0580028449.01	0.800912	1.153602e-48	5.436720e-46
MsG0280007876.01	MsG0580029148.01	0.851993	6.179732e-61	1.272837e-57
MsG0280007876.01	MsG0580029370.01	0.801040	1.086227e-48	5.135875e-46
MsG0280007876.01	MsG0580029661.01	0.800855	1.185074e-48	5.576827e-46
MsG0280007876.01	MsG0580030003.01	0.810532	1.099830e-50	6.646047e-48
MsG0280007876.01	MsG0580030160.01	0.831315	1.790096e-55	1.935425e-52
MsG0280007876.01	MsG0580030182.01	0.833014	6.801421e-56	7.734359e-53
MsG0280007876.01	MsG0580030235.01	0.811712	6.102384e-51	3.804710e-48
MsG0280007876.01	MsG0680031507.01	0.810157	1.325293e-50	7.929838e-48
MsG0280007876.01	MsG0680031508.01	0.839973	1.149709e-57	1.614413e-54
MsG0280007876.01	MsG0680031651.01	0.823663	1.226706e-53	1.063070e-50
MsG0280007876.01	MsG0680034061.01	0.819744	9.888783e-53	7.674987e-50
MsG0280007876.01	MsG0680034392.01	0.802435	5.617319e-49	2.751485e-46
MsG0280007876.01	MsG0680034395.01	0.800282	1.550850e-48	7.192724e-46
MsG0280007876.01	MsG0680034749.01	0.806204	9.216779e-50	4.973023e-47
MsG0280007876.01	MsG0680035306.01	0.810765	9.793953e-51	5.955604e-48
MsG0280007876.01	MsG0680035574.01	0.841228	5.397107e-58	7.877716e-55
MsG0280007876.01	MsG0780037755.01	0.801163	1.024779e-48	4.860328e-46
MsG0280007876.01	MsG0780038630.01	0.805943	1.045777e-49	5.605109e-47
MsG0280007876.01	MsG0780038837.01	0.837042	6.565138e-57	8.425256e-54
MsG0280007876.01	MsG0780038937.01	0.803741	3.015819e-49	1.527386e-46
MsG0280007876.01	MsG0780039570.01	0.810745	9.892747e-51	6.012469e-48
MsG0280007876.01	MsG0780039845.01	0.824497	7.816772e-54	6.935364e-51
MsG0280007876.01	MsG0780039950.01	0.821729	3.458314e-53	2.836652e-50
MsG0280007876.01	MsG0780040030.01	0.805745	1.150781e-49	6.136902e-47
MsG0280007876.01	MsG0780040216.01	0.822337	2.499959e-53	2.086553e-50
MsG0280007876.01	MsG0780040312.01	0.826258	2.994337e-54	2.793773e-51
MsG0280007876.01	MsG0780040411.01	0.863216	2.915680e-64	8.772132e-61
MsG0280007876.01	MsG0780040488.01	0.805492	1.300534e-49	6.890011e-47
MsG0280007876.01	MsG0780040519.01	0.823395	1.417400e-53	1.218804e-50
MsG0280007876.01	MsG0780040543.01	0.813109	3.022811e-51	1.956254e-48
MsG0280007876.01	MsG0780040597.01	0.801817	7.528750e-49	3.630099e-46
MsG0280007876.01	MsG0780040688.01	0.840550	8.126761e-58	1.161660e-54
MsG0280007876.01	MsG0780040845.01	0.859486	4.000520e-63	1.058276e-59
MsG0280007876.01	MsG0780040861.01	0.828991	6.607816e-55	6.673920e-52
MsG0280007876.01	MsG0780040880.01	0.827396	1.600738e-54	1.543686e-51
MsG0280007876.01	MsG0780040897.01	0.838635	2.558558e-57	3.447720e-54
MsG0280007876.01	MsG0780041295.01	0.802428	5.637494e-49	2.760918e-46
MsG0280007876.01	MsG0780041423.01	0.854535	1.153672e-61	2.584893e-58
MsG0280007876.01	MsG0780041469.01	0.847785	9.285095e-60	1.668573e-56
MsG0280007876.01	MsG0780041490.01	0.831912	1.275740e-55	1.404128e-52
MsG0280007876.01	MsG0780041565.01	0.863993	1.674350e-64	5.174985e-61
MsG0280007876.01	MsG0880042349.01	0.811019	8.631768e-51	5.284784e-48
MsG0280007876.01	MsG0880042552.01	0.853618	2.120893e-61	4.610529e-58
MsG0280007876.01	MsG0880042811.01	0.800608	1.330811e-48	6.223332e-46
MsG0280007876.01	MsG0880042829.01	0.823920	1.067924e-53	9.321616e-51
MsG0280007876.01	MsG0880042848.01	0.828340	9.494214e-55	9.409363e-52
MsG0280007876.01	MsG0880043532.01	0.802866	4.577907e-49	2.267086e-46
MsG0280007876.01	MsG0880043692.01	0.808877	2.495387e-50	1.443257e-47
MsG0280007876.01	MsG0880043886.01	0.801496	8.762003e-49	4.190583e-46
MsG0280007876.01	MsG0880044075.01	0.802583	5.237857e-49	2.575183e-46
MsG0280007876.01	MsG0880044792.01	0.818530	1.868352e-52	1.402110e-49
MsG0280007876.01	MsG0880044917.01	0.805561	1.257742e-49	6.675017e-47
MsG0280007876.01	MsG0880045619.01	0.823168	1.601177e-53	1.368218e-50
MsG0280007876.01	MsG0880046074.01	0.806268	8.934086e-50	4.828379e-47
MsG0280007876.01	MsG0880046086.01	0.804941	1.696572e-49	8.862398e-47
MsG0280007876.01	MsG0880046113.01	0.857202	1.917217e-62	4.694247e-59
MsG0280007876.01	MsG0880046177.01	0.845099	5.014682e-59	8.266554e-56
MsG0280007876.01	MsG0880046317.01	0.826754	2.280368e-54	2.157933e-51
MsG0280007876.01	MsG0880046445.01	0.801988	6.942798e-49	3.362085e-46
MsG0280007876.01	MsG0880046762.01	0.847849	8.914400e-60	1.605361e-56
MsG0280007876.01	MsG0880046933.01	0.860664	1.764512e-63	4.861159e-60
MsG0280007876.01	MsG0880047016.01	0.829789	4.228783e-55	4.373256e-52
MsG0280007876.01	MsG0880047120.01	0.808161	3.548976e-50	2.014553e-47
MsG0280007876.01	MsG0880047192.01	0.838944	2.128167e-57	2.894615e-54
MsG0280007876.01	MsG0880047590.01	0.801071	1.070247e-48	5.064234e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007876.01.T01	MTR_2g034640	99.065	107	1	0	12	118	87	193	3.87e-75	221
MsG0280007876.01.T01	MTR_7g112090	91.589	107	9	0	12	118	87	193	3.15e-71	211
MsG0280007876.01.T01	MTR_4g121860	93.458	107	7	0	12	118	87	193	3.25e-71	211
MsG0280007876.01.T01	MTR_4g026950	33.962	106	58	1	13	118	69	162	3.55e-12	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007876.01.T01	AT1G02620	94.393	107	6	0	12	118	16	122	5.33e-73	213
MsG0280007876.01.T01	AT4G02080	95.327	107	5	0	12	118	87	193	1.26e-72	214
MsG0280007876.01.T01	AT3G62560	93.396	106	7	0	12	117	87	192	3.18e-70	209
MsG0280007876.01.T01	AT1G56330	92.453	106	8	0	12	117	87	192	3.27e-69	206
MsG0280007876.01.T01	AT1G09180	87.850	107	13	0	12	118	87	193	7.68e-66	197
MsG0280007876.01.T01	AT3G49870	37.736	106	54	1	13	118	69	162	7.76e-15	67.0
MsG0280007876.01.T01	AT3G49870	37.736	106	54	1	13	118	88	181	1.84e-14	66.2
MsG0280007876.01.T01	AT5G67560	36.449	107	56	1	12	118	87	181	1.27e-13	64.3
MsG0280007876.01.T01	AT3G49860	46.970	66	35	0	13	78	80	145	3.35e-13	62.8
MsG0280007876.01.T01	AT2G18390	36.792	106	56	1	12	117	83	177	5.41e-12	59.7
MsG0280007876.01.T01	AT5G37680	46.154	65	35	0	13	77	41	105	2.65e-11	57.0
MsG0280007876.01.T01	AT5G37680	46.154	65	35	0	13	77	88	152	6.41e-11	57.0

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCTTTGCCTGTGGTGAAAT+TGG	0.182813	2:-22176457	None:intergenic
AATGTTCCATTTCTTATTCT+AGG	0.294978	2:+22176372	MsG0280007876.01.T01:CDS
TCGTCTTCTGAAGCTGCATA+TGG	0.388503	2:-22176411	None:intergenic
CTCACCTATGCTGGTTATCC+AGG	0.400198	2:+22176251	MsG0280007876.01.T01:CDS
TTTAGAATTCTCACCTATGC+TGG	0.444283	2:+22176242	MsG0280007876.01.T01:CDS
TTGTTTCCTAGAATAAGAAA+TGG	0.457150	2:-22176378	None:intergenic
TGCAGTATTGTCCGCAAGAT+GGG	0.469808	2:+22176528	MsG0280007876.01.T01:CDS
ATTGTCCGCAAGATGGGATA+TGG	0.471519	2:+22176534	MsG0280007876.01.T01:CDS
ACCAATTTCACCACAGGCAA+AGG	0.519780	2:+22176456	MsG0280007876.01.T01:CDS
CGTCTTCTGAAGCTGCATAT+GGG	0.521363	2:-22176410	None:intergenic
GTGCAGTATTGTCCGCAAGA+TGG	0.524469	2:+22176527	MsG0280007876.01.T01:CDS
TGATATACTGTGAAAGCCAC+TGG	0.530627	2:-22176566	None:intergenic
TGCCTGTGGTGAAATTGGTT+AGG	0.535024	2:-22176452	None:intergenic
GAGGTTTGCAGAATCTAAGA+AGG	0.538939	2:+22176314	MsG0280007876.01.T01:CDS
GGTAAACTACAGCGTCCACC+TGG	0.552429	2:-22176269	None:intergenic
ATTGACCTTGCCTTTGCCTG+TGG	0.559718	2:-22176466	None:intergenic
CACTTCAAGAGGTCGAACAT+TGG	0.562075	2:-22176499	None:intergenic
CGCAAGATGGGATATGGCGA+AGG	0.565211	2:+22176540	MsG0280007876.01.T01:CDS
AAGAAATGGAACATTTGCTA+AGG	0.577706	2:-22176364	None:intergenic
CTTTGTCGTAAGCATCGACT+AGG	0.589660	2:-22176290	None:intergenic
GGCCTAACCAATTTCACCAC+AGG	0.595827	2:+22176450	MsG0280007876.01.T01:CDS
CTGCACATGAACACTTCAAG+AGG	0.612208	2:-22176510	None:intergenic
TCGATGCTTACGACAAAGAG+AGG	0.612300	2:+22176295	MsG0280007876.01.T01:CDS
ACCTATGCTGGTTATCCAGG+TGG	0.614769	2:+22176254	MsG0280007876.01.T01:CDS
GACGAGCTACGATATCATCT+CGG	0.618823	2:+22176429	MsG0280007876.01.T01:CDS
TAAGGACTCATCTGAGAGCA+AGG	0.623056	2:-22176346	None:intergenic
TTTCACCACAGGCAAAGGCA+AGG	0.642687	2:+22176461	MsG0280007876.01.T01:CDS
CTTCGCCATATCCCATCTTG+CGG	0.645914	2:-22176539	None:intergenic
TCCACCTGGATAACCAGCAT+AGG	0.675478	2:-22176255	None:intergenic
GATATGGCGAAGGCTTCCAG+TGG	0.701402	2:+22176550	MsG0280007876.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGTTCCATTTCTTATTCT+AGG	+	Chr2:22176372-22176391	MsG0280007876.01.T01:CDS	25.0%
!	TTGTTTCCTAGAATAAGAAA+TGG	-	Chr2:22176381-22176400	None:intergenic	25.0%
!	AAGAAATGGAACATTTGCTA+AGG	-	Chr2:22176367-22176386	None:intergenic	30.0%
!	TTTAGAATTCTCACCTATGC+TGG	+	Chr2:22176242-22176261	MsG0280007876.01.T01:CDS	35.0%
	GAGGTTTGCAGAATCTAAGA+AGG	+	Chr2:22176314-22176333	MsG0280007876.01.T01:CDS	40.0%
	TGATATACTGTGAAAGCCAC+TGG	-	Chr2:22176569-22176588	None:intergenic	40.0%
	ACCAATTTCACCACAGGCAA+AGG	+	Chr2:22176456-22176475	MsG0280007876.01.T01:CDS	45.0%
	ATTGTCCGCAAGATGGGATA+TGG	+	Chr2:22176534-22176553	MsG0280007876.01.T01:CDS	45.0%
	CACTTCAAGAGGTCGAACAT+TGG	-	Chr2:22176502-22176521	None:intergenic	45.0%
	CTGCACATGAACACTTCAAG+AGG	-	Chr2:22176513-22176532	None:intergenic	45.0%
	GACGAGCTACGATATCATCT+CGG	+	Chr2:22176429-22176448	MsG0280007876.01.T01:CDS	45.0%
	TAAGGACTCATCTGAGAGCA+AGG	-	Chr2:22176349-22176368	None:intergenic	45.0%
	TCGATGCTTACGACAAAGAG+AGG	+	Chr2:22176295-22176314	MsG0280007876.01.T01:CDS	45.0%
	TGCAGTATTGTCCGCAAGAT+GGG	+	Chr2:22176528-22176547	MsG0280007876.01.T01:CDS	45.0%
	TGCCTGTGGTGAAATTGGTT+AGG	-	Chr2:22176455-22176474	None:intergenic	45.0%
!!	CGTCTTCTGAAGCTGCATAT+GGG	-	Chr2:22176413-22176432	None:intergenic	45.0%
!!	CTTTGTCGTAAGCATCGACT+AGG	-	Chr2:22176293-22176312	None:intergenic	45.0%
!!	TCGTCTTCTGAAGCTGCATA+TGG	-	Chr2:22176414-22176433	None:intergenic	45.0%
	ATTGACCTTGCCTTTGCCTG+TGG	-	Chr2:22176469-22176488	None:intergenic	50.0%
	CTTCGCCATATCCCATCTTG+CGG	-	Chr2:22176542-22176561	None:intergenic	50.0%
	GCCTTTGCCTGTGGTGAAAT+TGG	-	Chr2:22176460-22176479	None:intergenic	50.0%
	GGCCTAACCAATTTCACCAC+AGG	+	Chr2:22176450-22176469	MsG0280007876.01.T01:CDS	50.0%
	GTGCAGTATTGTCCGCAAGA+TGG	+	Chr2:22176527-22176546	MsG0280007876.01.T01:CDS	50.0%
	TCCACCTGGATAACCAGCAT+AGG	-	Chr2:22176258-22176277	None:intergenic	50.0%
	TTTCACCACAGGCAAAGGCA+AGG	+	Chr2:22176461-22176480	MsG0280007876.01.T01:CDS	50.0%
!	ACCTATGCTGGTTATCCAGG+TGG	+	Chr2:22176254-22176273	MsG0280007876.01.T01:CDS	50.0%
!	CTCACCTATGCTGGTTATCC+AGG	+	Chr2:22176251-22176270	MsG0280007876.01.T01:CDS	50.0%
	CGCAAGATGGGATATGGCGA+AGG	+	Chr2:22176540-22176559	MsG0280007876.01.T01:CDS	55.0%
	GATATGGCGAAGGCTTCCAG+TGG	+	Chr2:22176550-22176569	MsG0280007876.01.T01:CDS	55.0%
	GGTAAACTACAGCGTCCACC+TGG	-	Chr2:22176272-22176291	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	22176237	22176593	22176237	ID=MsG0280007876.01;Name=MsG0280007876.01
Chr2	mRNA	22176237	22176593	22176237	ID=MsG0280007876.01.T01;Parent=MsG0280007876.01;Name=MsG0280007876.01.T01;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|118
Chr2	exon	22176237	22176593	22176237	ID=MsG0280007876.01.T01:exon:2883;Parent=MsG0280007876.01.T01
Chr2	CDS	22176237	22176593	22176237	ID=MsG0280007876.01.T01:cds;Parent=MsG0280007876.01.T01

Gene Sequence	>MsG0280007876.01.T01 ATGGCTTTAGAATTCTCACCTATGCTGGTTATCCAGGTGGACGCTGTAGTTTACCTAGTCGATGCTTACGACAAAGAGAGGTTTGCAGAATCTAAGAAGGAGCTTGACGCCTTGCTCTCAGATGAGTCCTTAGCAAATGTTCCATTTCTTATTCTAGGAAACAAGATTGACATCCCATATGCAGCTTCAGAAGACGAGCTACGATATCATCTCGGCCTAACCAATTTCACCACAGGCAAAGGCAAGGTCAATCTTACAGACTCCAATGTTCGACCTCTTGAAGTGTTCATGTGCAGTATTGTCCGCAAGATGGGATATGGCGAAGGCTTCCAGTGGCTTTCACAGTATATCAAGTAG
Protein sequence	>MsG0280007876.01.T01 MALEFSPMLVIQVDAVVYLVDAYDKERFAESKKELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGLTNFTTGKGKVNLTDSNVRPLEVFMCSIVRKMGYGEGFQWLSQYIK*