Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043607.01.T01 | XP_003627814.1 | 96.61 | 177 | 4 | 1 | 1 | 177 | 3 | 177 | 6.70E-119 | 343 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043607.01.T01 | Q2RAP3 | 71.069 | 159 | 41 | 2 | 1 | 158 | 3 | 157 | 9.38E-75 | 225 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043607.01.T01 | Q1RU46 | 96.610 | 177 | 4 | 1 | 1 | 177 | 3 | 177 | 3.20e-119 | 343 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880043607.01.T01 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048889.01 | MsG0880043607.01 | 0.821724 | 3.466829e-53 | 2.843273e-50 |
| MsG0080048980.01 | MsG0880043607.01 | 0.819116 | 1.374864e-52 | 1.048606e-49 |
| MsG0080049066.01 | MsG0880043607.01 | -0.806291 | 8.835599e-50 | 4.777800e-47 |
| MsG0180000287.01 | MsG0880043607.01 | 0.809415 | 1.913755e-50 | 1.122977e-47 |
| MsG0180001497.01 | MsG0880043607.01 | 0.810947 | 8.946355e-51 | 5.467138e-48 |
| MsG0180001513.01 | MsG0880043607.01 | 0.818778 | 1.641126e-52 | 1.240046e-49 |
| MsG0180002935.01 | MsG0880043607.01 | 0.802194 | 6.297540e-49 | 3.065621e-46 |
| MsG0180003375.01 | MsG0880043607.01 | 0.822068 | 2.886043e-53 | 2.390088e-50 |
| MsG0180003484.01 | MsG0880043607.01 | 0.823629 | 1.249833e-53 | 1.082018e-50 |
| MsG0180005417.01 | MsG0880043607.01 | 0.819914 | 9.041646e-53 | 7.051252e-50 |
| MsG0780040498.01 | MsG0880043607.01 | 0.809927 | 1.485113e-50 | 8.833191e-48 |
| MsG0780040977.01 | MsG0880043607.01 | 0.800570 | 1.354963e-48 | 6.330071e-46 |
| MsG0780041036.01 | MsG0880043607.01 | 0.821180 | 4.631068e-53 | 3.740865e-50 |
| MsG0780041180.01 | MsG0880043607.01 | 0.817051 | 4.030264e-52 | 2.904170e-49 |
| MsG0780041397.01 | MsG0880043607.01 | 0.811563 | 6.577413e-51 | 4.084895e-48 |
| MsG0880042122.01 | MsG0880043607.01 | 0.815875 | 7.388530e-52 | 5.155119e-49 |
| MsG0880042740.01 | MsG0880043607.01 | 0.805847 | 1.095236e-49 | 5.855900e-47 |
| MsG0880043212.01 | MsG0880043607.01 | 0.800076 | 1.707674e-48 | 7.878640e-46 |
| MsG0880043364.01 | MsG0880043607.01 | 0.804843 | 1.777947e-49 | 9.264059e-47 |
| MsG0880043481.01 | MsG0880043607.01 | 0.808573 | 2.898766e-50 | 1.663263e-47 |
| MsG0880043550.01 | MsG0880043607.01 | 0.812553 | 4.000744e-51 | 2.551558e-48 |
| MsG0880043607.01 | MsG0880043998.01 | 0.807324 | 5.344032e-50 | 2.967792e-47 |
| MsG0880043607.01 | MsG0880045854.01 | 0.808476 | 3.040071e-50 | 1.740027e-47 |
| MsG0880043607.01 | MsG0880046271.01 | 0.809953 | 1.466699e-50 | 8.729540e-48 |
| MsG0880043607.01 | MsG0880047714.01 | 0.824626 | 7.290482e-54 | 6.493631e-51 |
| MsG0280011236.01 | MsG0880043607.01 | 0.823447 | 1.378393e-53 | 1.187109e-50 |
| MsG0280011267.01 | MsG0880043607.01 | 0.819710 | 1.006612e-52 | 7.805453e-50 |
| MsG0280011269.01 | MsG0880043607.01 | 0.807999 | 3.840547e-50 | 2.170726e-47 |
| MsG0380011974.01 | MsG0880043607.01 | 0.821945 | 3.081664e-53 | 2.543257e-50 |
| MsG0380012889.01 | MsG0880043607.01 | 0.833816 | 4.291896e-56 | 4.999176e-53 |
| MsG0380012900.01 | MsG0880043607.01 | 0.818630 | 1.773551e-52 | 1.334563e-49 |
| MsG0380013272.01 | MsG0880043607.01 | 0.809191 | 2.137265e-50 | 1.246659e-47 |
| MsG0380013285.01 | MsG0880043607.01 | 0.809850 | 1.543543e-50 | 9.161927e-48 |
| MsG0380013415.01 | MsG0880043607.01 | 0.800669 | 1.292931e-48 | 6.055730e-46 |
| MsG0380014639.01 | MsG0880043607.01 | -0.805068 | 1.595403e-49 | 8.360863e-47 |
| MsG0380014642.01 | MsG0880043607.01 | -0.807465 | 4.988794e-50 | 2.780716e-47 |
| MsG0680030960.01 | MsG0880043607.01 | 0.805401 | 1.358760e-49 | 7.181798e-47 |
| MsG0680031841.01 | MsG0880043607.01 | 0.825068 | 5.733483e-54 | 5.171438e-51 |
| MsG0680033347.01 | MsG0880043607.01 | 0.806866 | 6.679056e-50 | 3.665409e-47 |
| MsG0680033467.01 | MsG0880043607.01 | 0.814908 | 1.212567e-51 | 8.239455e-49 |
| MsG0680034418.01 | MsG0880043607.01 | 0.813057 | 3.103935e-51 | 2.006045e-48 |
| MsG0680034953.01 | MsG0880043607.01 | 0.826021 | 3.409639e-54 | 3.159930e-51 |
| MsG0680034978.01 | MsG0880043607.01 | 0.804346 | 2.257707e-49 | 1.161339e-46 |
| MsG0680035584.01 | MsG0880043607.01 | -0.811493 | 6.810237e-51 | 4.221870e-48 |
| MsG0480019545.01 | MsG0880043607.01 | 0.803733 | 3.028543e-49 | 1.533489e-46 |
| MsG0480019666.01 | MsG0880043607.01 | 0.808332 | 3.262157e-50 | 1.860151e-47 |
| MsG0480019842.01 | MsG0880043607.01 | 0.814607 | 1.413526e-51 | 9.528037e-49 |
| MsG0480020722.01 | MsG0880043607.01 | 0.810862 | 9.331855e-51 | 5.689457e-48 |
| MsG0480021129.01 | MsG0880043607.01 | 0.807723 | 4.396868e-50 | 2.467270e-47 |
| MsG0480021133.01 | MsG0880043607.01 | 0.821540 | 3.825177e-53 | 3.120831e-50 |
| MsG0480021396.01 | MsG0880043607.01 | 0.823816 | 1.129692e-53 | 9.832382e-51 |
| MsG0480021433.01 | MsG0880043607.01 | 0.812672 | 3.767862e-51 | 2.410514e-48 |
| MsG0480021573.01 | MsG0880043607.01 | 0.809166 | 2.163693e-50 | 1.261222e-47 |
| MsG0480022310.01 | MsG0880043607.01 | 0.805460 | 1.321045e-49 | 6.993056e-47 |
| MsG0480022428.01 | MsG0880043607.01 | 0.850092 | 2.122437e-60 | 4.110919e-57 |
| MsG0480022833.01 | MsG0880043607.01 | 0.804543 | 2.053748e-49 | 1.061764e-46 |
| MsG0480023067.01 | MsG0880043607.01 | 0.809084 | 2.253164e-50 | 1.310483e-47 |
| MsG0480023322.01 | MsG0880043607.01 | 0.802106 | 6.566044e-49 | 3.189237e-46 |
| MsG0580024218.01 | MsG0880043607.01 | 0.823416 | 1.401318e-53 | 1.205719e-50 |
| MsG0580024219.01 | MsG0880043607.01 | 0.800026 | 1.748223e-48 | 8.055374e-46 |
| MsG0580024220.01 | MsG0880043607.01 | 0.801426 | 9.053903e-49 | 4.322496e-46 |
| MsG0580025023.01 | MsG0880043607.01 | 0.824475 | 7.909823e-54 | 7.013356e-51 |
| MsG0580025117.01 | MsG0880043607.01 | 0.800617 | 1.324798e-48 | 6.196755e-46 |
| MsG0580025431.01 | MsG0880043607.01 | 0.802710 | 4.929281e-49 | 2.431475e-46 |
| MsG0580025432.01 | MsG0880043607.01 | 0.818812 | 1.612607e-52 | 1.219567e-49 |
| MsG0580025486.01 | MsG0880043607.01 | -0.803445 | 3.474145e-49 | 1.746149e-46 |
| MsG0380015323.01 | MsG0880043607.01 | 0.805781 | 1.131236e-49 | 6.037987e-47 |
| MsG0380016372.01 | MsG0880043607.01 | 0.806864 | 6.686906e-50 | 3.669483e-47 |
| MsG0380016935.01 | MsG0880043607.01 | -0.804366 | 2.236376e-49 | 1.150946e-46 |
| MsG0380017405.01 | MsG0880043607.01 | -0.800828 | 1.200048e-48 | 5.643225e-46 |
| MsG0580027337.01 | MsG0880043607.01 | 0.804743 | 1.865870e-49 | 9.697246e-47 |
| MsG0580028302.01 | MsG0880043607.01 | 0.803020 | 4.255440e-49 | 2.115498e-46 |
| MsG0680030635.01 | MsG0880043607.01 | -0.804111 | 2.527139e-49 | 1.292104e-46 |
| MsG0280007426.01 | MsG0880043607.01 | 0.802986 | 4.324041e-49 | 2.147826e-46 |
| MsG0280007786.01 | MsG0880043607.01 | 0.803623 | 3.191604e-49 | 1.611486e-46 |
| MsG0280007937.01 | MsG0880043607.01 | 0.807368 | 5.229319e-50 | 2.907317e-47 |
| MsG0280009167.01 | MsG0880043607.01 | 0.807812 | 4.210364e-50 | 2.368001e-47 |
| MsG0280009232.01 | MsG0880043607.01 | 0.800331 | 1.515480e-48 | 7.037537e-46 |
| MsG0280009742.01 | MsG0880043607.01 | -0.801930 | 7.138021e-49 | 3.451510e-46 |
| MsG0780036199.01 | MsG0880043607.01 | 0.816309 | 5.910442e-52 | 4.173537e-49 |
| MsG0780036208.01 | MsG0880043607.01 | 0.805514 | 1.286817e-49 | 6.821025e-47 |
| MsG0780037813.01 | MsG0880043607.01 | 0.810532 | 1.100188e-50 | 6.648126e-48 |
| MsG0780038305.01 | MsG0880043607.01 | 0.824387 | 8.297564e-54 | 7.338535e-51 |
| MsG0780038307.01 | MsG0880043607.01 | 0.822897 | 1.852548e-53 | 1.570932e-50 |
| MsG0780038519.01 | MsG0880043607.01 | 0.808998 | 2.350963e-50 | 1.364140e-47 |
| MsG0780039431.01 | MsG0880043607.01 | 0.815655 | 8.269562e-52 | 5.735759e-49 |
| MsG0780039619.01 | MsG0880043607.01 | 0.803864 | 2.844579e-49 | 1.445258e-46 |
| MsG0780039855.01 | MsG0880043607.01 | 0.802609 | 5.171585e-49 | 2.544361e-46 |
| MsG0780039883.01 | MsG0880043607.01 | 0.826047 | 3.360208e-54 | 3.116443e-51 |
| MsG0780039916.01 | MsG0880043607.01 | 0.810730 | 9.967956e-51 | 6.055737e-48 |
| MsG0780039923.01 | MsG0880043607.01 | 0.808720 | 2.696054e-50 | 1.552833e-47 |
| MsG0780039979.01 | MsG0880043607.01 | 0.826373 | 2.811030e-54 | 2.631503e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043607.01.T01 | MTR_8g038620 | 96.610 | 177 | 4 | 1 | 1 | 177 | 3 | 177 | 8.11e-123 | 343 |
| MsG0880043607.01.T01 | MTR_7g490310 | 72.727 | 154 | 41 | 1 | 1 | 153 | 3 | 156 | 1.31e-79 | 236 |
| MsG0880043607.01.T01 | MTR_6g007460 | 59.809 | 209 | 23 | 3 | 1 | 156 | 5 | 205 | 2.89e-76 | 227 |
| MsG0880043607.01.T01 | MTR_3g015490 | 76.433 | 157 | 30 | 4 | 6 | 160 | 3 | 154 | 9.68e-76 | 224 |
| MsG0880043607.01.T01 | MTR_3g088630 | 58.125 | 160 | 56 | 2 | 4 | 153 | 19 | 177 | 5.27e-59 | 184 |
| MsG0880043607.01.T01 | MTR_5g036480 | 58.451 | 142 | 55 | 1 | 6 | 147 | 15 | 152 | 7.65e-53 | 168 |
| MsG0880043607.01.T01 | MTR_4g106590 | 54.438 | 169 | 64 | 4 | 2 | 168 | 21 | 178 | 4.72e-52 | 166 |
| MsG0880043607.01.T01 | MTR_1g049100 | 59.859 | 142 | 51 | 2 | 6 | 147 | 7 | 142 | 2.86e-51 | 163 |
| MsG0880043607.01.T01 | MTR_3g078613 | 52.174 | 161 | 60 | 3 | 2 | 154 | 18 | 169 | 7.00e-51 | 162 |
| MsG0880043607.01.T01 | MTR_3g078613 | 51.852 | 162 | 60 | 4 | 2 | 154 | 18 | 170 | 4.81e-49 | 157 |
| MsG0880043607.01.T01 | MTR_3g093860 | 57.246 | 138 | 49 | 3 | 8 | 143 | 19 | 148 | 1.41e-48 | 155 |
| MsG0880043607.01.T01 | MTR_4g051330 | 82.353 | 68 | 11 | 1 | 93 | 160 | 77 | 143 | 9.00e-31 | 109 |
| MsG0880043607.01.T01 | MTR_8g079940 | 29.193 | 161 | 90 | 4 | 9 | 165 | 31 | 171 | 3.19e-15 | 72.8 |
| MsG0880043607.01.T01 | MTR_8g024260 | 28.777 | 139 | 82 | 1 | 9 | 147 | 38 | 159 | 8.19e-15 | 71.6 |
| MsG0880043607.01.T01 | MTR_4g061360 | 30.882 | 136 | 77 | 2 | 9 | 144 | 91 | 209 | 8.03e-14 | 68.9 |
| MsG0880043607.01.T01 | MTR_7g117705 | 30.435 | 138 | 77 | 3 | 9 | 146 | 11 | 129 | 6.83e-13 | 66.2 |
| MsG0880043607.01.T01 | MTR_3g086100 | 27.273 | 165 | 93 | 4 | 9 | 166 | 19 | 163 | 8.63e-13 | 65.9 |
| MsG0880043607.01.T01 | MTR_3g092780 | 27.703 | 148 | 90 | 2 | 9 | 156 | 58 | 188 | 1.69e-12 | 65.1 |
| MsG0880043607.01.T01 | MTR_1g032570 | 28.276 | 145 | 84 | 3 | 9 | 153 | 17 | 141 | 2.83e-12 | 64.3 |
| MsG0880043607.01.T01 | MTR_1g013170 | 29.710 | 138 | 77 | 3 | 9 | 146 | 11 | 128 | 5.37e-12 | 63.5 |
| MsG0880043607.01.T01 | MTR_1g013180 | 27.429 | 175 | 96 | 6 | 9 | 172 | 162 | 316 | 7.56e-12 | 63.2 |
| MsG0880043607.01.T01 | MTR_3g092780 | 28.058 | 139 | 83 | 2 | 9 | 147 | 58 | 179 | 9.24e-12 | 62.8 |
| MsG0880043607.01.T01 | MTR_4g131580 | 27.108 | 166 | 100 | 4 | 9 | 174 | 23 | 167 | 1.09e-11 | 62.8 |
| MsG0880043607.01.T01 | MTR_7g118260 | 26.897 | 145 | 82 | 3 | 13 | 152 | 22 | 147 | 1.51e-11 | 62.4 |
| MsG0880043607.01.T01 | MTR_3g092780 | 26.000 | 150 | 94 | 1 | 7 | 156 | 56 | 188 | 1.67e-11 | 62.4 |
| MsG0880043607.01.T01 | MTR_7g118260 | 27.273 | 132 | 77 | 2 | 13 | 144 | 22 | 134 | 2.29e-11 | 61.6 |
| MsG0880043607.01.T01 | MTR_3g106220 | 27.607 | 163 | 89 | 3 | 9 | 171 | 14 | 147 | 2.96e-11 | 61.6 |
| MsG0880043607.01.T01 | MTR_4g121020 | 27.326 | 172 | 94 | 5 | 7 | 176 | 35 | 177 | 4.50e-11 | 60.8 |
| MsG0880043607.01.T01 | MTR_4g121020 | 27.326 | 172 | 94 | 5 | 7 | 176 | 35 | 177 | 4.50e-11 | 60.8 |
| MsG0880043607.01.T01 | MTR_3g092780 | 26.429 | 140 | 86 | 1 | 7 | 146 | 56 | 178 | 8.43e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043607.01.T01 | AT2G41310 | 53.488 | 215 | 59 | 1 | 1 | 174 | 3 | 217 | 6.23e-75 | 224 |
| MsG0880043607.01.T01 | AT3G57040 | 65.455 | 165 | 49 | 2 | 1 | 160 | 3 | 164 | 4.49e-72 | 217 |
| MsG0880043607.01.T01 | AT3G57040 | 65.455 | 165 | 49 | 2 | 1 | 160 | 3 | 164 | 4.49e-72 | 217 |
| MsG0880043607.01.T01 | AT1G59940 | 61.429 | 140 | 46 | 2 | 5 | 144 | 31 | 162 | 8.74e-56 | 176 |
| MsG0880043607.01.T01 | AT1G59940 | 61.429 | 140 | 46 | 2 | 5 | 144 | 123 | 254 | 5.80e-54 | 174 |
| MsG0880043607.01.T01 | AT1G10470 | 60.000 | 140 | 48 | 1 | 5 | 144 | 32 | 163 | 8.06e-54 | 172 |
| MsG0880043607.01.T01 | AT1G10470 | 60.000 | 140 | 48 | 1 | 5 | 144 | 18 | 149 | 8.23e-54 | 171 |
| MsG0880043607.01.T01 | AT3G48100 | 57.143 | 147 | 54 | 2 | 1 | 146 | 19 | 157 | 1.01e-53 | 169 |
| MsG0880043607.01.T01 | AT5G62920 | 57.931 | 145 | 51 | 2 | 1 | 144 | 19 | 154 | 1.14e-52 | 166 |
| MsG0880043607.01.T01 | AT1G19050 | 53.548 | 155 | 64 | 1 | 6 | 160 | 23 | 169 | 3.50e-52 | 166 |
| MsG0880043607.01.T01 | AT3G56380 | 53.793 | 145 | 60 | 1 | 2 | 146 | 15 | 152 | 7.46e-52 | 163 |
| MsG0880043607.01.T01 | AT1G74890 | 55.172 | 145 | 57 | 1 | 6 | 150 | 17 | 153 | 1.84e-50 | 161 |
| MsG0880043607.01.T01 | AT2G40670 | 53.901 | 141 | 60 | 2 | 6 | 146 | 28 | 163 | 2.52e-50 | 159 |
| MsG0880043607.01.T01 | AT3G56380 | 53.425 | 146 | 60 | 2 | 2 | 146 | 15 | 153 | 4.05e-50 | 159 |
| MsG0880043607.01.T01 | AT2G40670 | 53.521 | 142 | 60 | 3 | 6 | 146 | 28 | 164 | 1.60e-48 | 155 |
| MsG0880043607.01.T01 | AT2G07440 | 45.833 | 120 | 27 | 1 | 93 | 174 | 9 | 128 | 3.46e-26 | 97.4 |
| MsG0880043607.01.T01 | AT1G10470 | 64.151 | 53 | 19 | 0 | 92 | 144 | 35 | 87 | 6.71e-18 | 77.4 |
| MsG0880043607.01.T01 | AT1G67710 | 29.167 | 144 | 82 | 3 | 9 | 152 | 13 | 136 | 3.36e-13 | 67.0 |
| MsG0880043607.01.T01 | AT5G60100 | 31.618 | 136 | 76 | 2 | 9 | 144 | 66 | 184 | 3.69e-13 | 67.0 |
| MsG0880043607.01.T01 | AT5G60100 | 31.618 | 136 | 76 | 2 | 9 | 144 | 66 | 184 | 3.69e-13 | 67.0 |
| MsG0880043607.01.T01 | AT5G60100 | 31.618 | 136 | 76 | 2 | 9 | 144 | 66 | 184 | 3.69e-13 | 67.0 |
| MsG0880043607.01.T01 | AT5G07210 | 31.655 | 139 | 74 | 3 | 8 | 146 | 17 | 134 | 4.27e-13 | 67.0 |
| MsG0880043607.01.T01 | AT5G60100 | 31.618 | 136 | 76 | 2 | 9 | 144 | 66 | 184 | 4.39e-13 | 66.6 |
| MsG0880043607.01.T01 | AT5G60100 | 31.618 | 136 | 76 | 2 | 9 | 144 | 66 | 184 | 6.66e-13 | 66.2 |
| MsG0880043607.01.T01 | AT5G60100 | 31.618 | 136 | 76 | 2 | 9 | 144 | 66 | 184 | 6.66e-13 | 66.2 |
| MsG0880043607.01.T01 | AT5G60100 | 31.618 | 136 | 76 | 2 | 9 | 144 | 66 | 184 | 6.66e-13 | 66.2 |
| MsG0880043607.01.T01 | AT2G25180 | 27.742 | 155 | 88 | 4 | 1 | 151 | 7 | 141 | 3.14e-12 | 64.3 |
| MsG0880043607.01.T01 | AT5G02810 | 27.941 | 136 | 81 | 2 | 9 | 144 | 80 | 198 | 3.39e-12 | 64.3 |
| MsG0880043607.01.T01 | AT5G24470 | 27.857 | 140 | 80 | 3 | 9 | 147 | 54 | 173 | 3.92e-11 | 61.2 |
Find 36 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATAGCTGAACTGGTTTC+AGG | 0.198335 | 8:+27680823 | None:intergenic |
| AGTAAGGCTTTAAAATTTCT+TGG | 0.266953 | 8:-27681787 | MsG0880043607.01.T01:CDS |
| AGGTATGTCTTTGAAAGATT+TGG | 0.295699 | 8:+27681072 | None:intergenic |
| AGAGAATCAGTTCCATGTTT+TGG | 0.307421 | 8:-27682065 | MsG0880043607.01.T01:CDS |
| GGCTTTAAAATTTCTTGGTT+TGG | 0.308229 | 8:-27681782 | MsG0880043607.01.T01:CDS |
| AAAATTTAAATGCTAACTTT+TGG | 0.330093 | 8:+27680684 | None:intergenic |
| TCTCACCTATTGATCCTAGA+TGG | 0.331221 | 8:+27681022 | None:intergenic |
| TACAGTGGATTCTGCTAGTA+AGG | 0.383079 | 8:-27681803 | MsG0880043607.01.T01:CDS |
| TATTGCTTCAGAAATTAATC+AGG | 0.389954 | 8:-27681728 | MsG0880043607.01.T01:intron |
| TGATTAATTTCTGAAGCAAT+AGG | 0.390281 | 8:+27681730 | None:intergenic |
| CTTACATCTGATAGCTGAAC+TGG | 0.391326 | 8:+27680816 | None:intergenic |
| ATGTTCCATCTAGGATCAAT+AGG | 0.432186 | 8:-27681027 | MsG0880043607.01.T01:intron |
| AAAATTTCTTGGTTTGGTTG+AGG | 0.449963 | 8:-27681776 | MsG0880043607.01.T01:CDS |
| ATAACAGATTATTGTATGCC+AGG | 0.463952 | 8:-27681594 | MsG0880043607.01.T01:CDS |
| TTTGTTGAAATCAAAAGTTA+AGG | 0.471711 | 8:-27680778 | MsG0880043607.01.T01:CDS |
| AACAACCTATCAACATCAAA+AGG | 0.471937 | 8:-27680746 | MsG0880043607.01.T01:CDS |
| TTGTAATTGTATCAGATGTT+TGG | 0.481004 | 8:-27680868 | MsG0880043607.01.T01:intron |
| TCTGAGGACATTATCACAAC+AGG | 0.486527 | 8:+27681052 | None:intergenic |
| CAACATCAAAAGGAAGGGTA+TGG | 0.487124 | 8:-27680736 | MsG0880043607.01.T01:CDS |
| CCTCAGAGAATGTTCCATCT+AGG | 0.494035 | 8:-27681036 | MsG0880043607.01.T01:CDS |
| TATTGTATGCCAGGGATGAC+CGG | 0.500365 | 8:-27681585 | MsG0880043607.01.T01:CDS |
| TGTATCAGATGTTTGGAAGA+GGG | 0.502137 | 8:-27680861 | MsG0880043607.01.T01:intron |
| TTGTATCAGATGTTTGGAAG+AGG | 0.508259 | 8:-27680862 | MsG0880043607.01.T01:intron |
| TGAAGCAATAGGAGTGTCAT+AGG | 0.512512 | 8:+27681741 | None:intergenic |
| TTGATAGAATGTTGATTGAG+AGG | 0.516155 | 8:-27682024 | MsG0880043607.01.T01:CDS |
| AGATCATAGCCGGTCATCCC+TGG | 0.522997 | 8:+27681576 | None:intergenic |
| ACCTATCAACATCAAAAGGA+AGG | 0.530682 | 8:-27680742 | MsG0880043607.01.T01:CDS |
| TAACAGATTATTGTATGCCA+GGG | 0.564090 | 8:-27681593 | MsG0880043607.01.T01:CDS |
| TATCATCAACAGCCAAAACA+TGG | 0.580138 | 8:+27682053 | None:intergenic |
| GTCAATCACCAGATAAAACT+AGG | 0.591896 | 8:-27680707 | MsG0880043607.01.T01:CDS |
| GTATCAGATGTTTGGAAGAG+GGG | 0.607171 | 8:-27680860 | MsG0880043607.01.T01:intron |
| TATCAGATGTTTGGAAGAGG+GGG | 0.611980 | 8:-27680859 | MsG0880043607.01.T01:intron |
| TTGAAATACAGTTACTACAG+TGG | 0.620208 | 8:-27681818 | MsG0880043607.01.T01:intron |
| CCTATCAACATCAAAAGGAA+GGG | 0.630110 | 8:-27680741 | MsG0880043607.01.T01:CDS |
| GTTTGGTTGAGGATGAAATG+AGG | 0.636842 | 8:-27681765 | MsG0880043607.01.T01:CDS |
| CCTAGATGGAACATTCTCTG+AGG | 0.707424 | 8:+27681036 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACAAATTTATACAATAAAA+TGG | + | Chr8:27681099-27681118 | None:intergenic | 10.0% |
| !!! | AGATTAGTTAATTGAAAATT+TGG | + | Chr8:27681538-27681557 | None:intergenic | 15.0% |
| !!! | ATTATTTTGCTTTTCTATTT+AGG | - | Chr8:27681370-27681389 | MsG0880043607.01.T01:intron | 15.0% |
| !!! | GATTAGTTAATTGAAAATTT+GGG | + | Chr8:27681537-27681556 | None:intergenic | 15.0% |
| !! | TACTGAATTGATAAAGATTA+TGG | - | Chr8:27681067-27681086 | MsG0880043607.01.T01:CDS | 20.0% |
| !! | TAGGAAACAATTTGAAAAAT+AGG | - | Chr8:27681389-27681408 | MsG0880043607.01.T01:intron | 20.0% |
| !! | TTTGTTGAAATCAAAAGTTA+AGG | - | Chr8:27681984-27682003 | MsG0880043607.01.T01:intron | 20.0% |
| !!! | AAATGCAAAGTCTTATATTA+TGG | - | Chr8:27681835-27681854 | MsG0880043607.01.T01:intron | 20.0% |
| !!! | ACAATTACAAACAATTTGTT+AGG | + | Chr8:27681884-27681903 | None:intergenic | 20.0% |
| !!! | ATAGTCTATTATTTTGTTGA+AGG | - | Chr8:27680864-27680883 | MsG0880043607.01.T01:intron | 20.0% |
| !!! | GATCTTATTTCTTAAATACA+TGG | - | Chr8:27681411-27681430 | MsG0880043607.01.T01:intron | 20.0% |
| ! | ATACTAGATTAATCGTGTTA+TGG | + | Chr8:27681776-27681795 | None:intergenic | 25.0% |
| ! | ATCAATTCTCACAATTGATT+TGG | - | Chr8:27681504-27681523 | MsG0880043607.01.T01:intron | 25.0% |
| ! | TAGACTAATCAAATAAGAAG+TGG | + | Chr8:27681274-27681293 | None:intergenic | 25.0% |
| ! | TATTGCTTCAGAAATTAATC+AGG | - | Chr8:27681034-27681053 | MsG0880043607.01.T01:CDS | 25.0% |
| ! | TTGAATGCATCAAAAAAGTT+TGG | - | Chr8:27681343-27681362 | MsG0880043607.01.T01:intron | 25.0% |
| ! | TTGTAATTGTATCAGATGTT+TGG | - | Chr8:27681894-27681913 | MsG0880043607.01.T01:intron | 25.0% |
| ! | TTTGATTCAATTATTCATGC+AGG | - | Chr8:27681125-27681144 | MsG0880043607.01.T01:intron | 25.0% |
| !! | CATTAAAAGAAGAAGTTTTG+AGG | + | Chr8:27680766-27680785 | None:intergenic | 25.0% |
| !! | CTCAAAACTTCTTCTTTTAA+TGG | - | Chr8:27680764-27680783 | MsG0880043607.01.T01:CDS | 25.0% |
| !!! | ACTTTTGATTTCAACAAATG+TGG | + | Chr8:27681982-27682001 | None:intergenic | 25.0% |
| !!! | AGTAAGGCTTTAAAATTTCT+TGG | - | Chr8:27680975-27680994 | MsG0880043607.01.T01:intron | 25.0% |
| !!! | TGATTAATTTCTGAAGCAAT+AGG | + | Chr8:27681035-27681054 | None:intergenic | 25.0% |
| !!! | TTTGTTGAAGGTGTTTTTTT+TGG | - | Chr8:27680876-27680895 | MsG0880043607.01.T01:intron | 25.0% |
| AACAACCTATCAACATCAAA+AGG | - | Chr8:27682016-27682035 | MsG0880043607.01.T01:CDS | 30.0% | |
| AATAAAGTTCATTGATCTGC+AGG | - | Chr8:27681664-27681683 | MsG0880043607.01.T01:intron | 30.0% | |
| ATAACAGATTATTGTATGCC+AGG | - | Chr8:27681168-27681187 | MsG0880043607.01.T01:intron | 30.0% | |
| CAAAAGTAAGCACAAAATTG+TGG | + | Chr8:27681319-27681338 | None:intergenic | 30.0% | |
| TAACAGATTATTGTATGCCA+GGG | - | Chr8:27681169-27681188 | MsG0880043607.01.T01:intron | 30.0% | |
| TCTCAATCCAAAATTGTTCA+TGG | - | Chr8:27681555-27681574 | MsG0880043607.01.T01:intron | 30.0% | |
| TGATCTGTTGAGAAAAATCA+AGG | - | Chr8:27681203-27681222 | MsG0880043607.01.T01:intron | 30.0% | |
| TTGAAATACAGTTACTACAG+TGG | - | Chr8:27680944-27680963 | MsG0880043607.01.T01:intron | 30.0% | |
| ! | AGGTATGTCTTTGAAAGATT+TGG | + | Chr8:27681693-27681712 | None:intergenic | 30.0% |
| ! | ATGCTTTCCATGAACAATTT+TGG | + | Chr8:27681565-27681584 | None:intergenic | 30.0% |
| ! | TTGATAGAATGTTGATTGAG+AGG | - | Chr8:27680738-27680757 | MsG0880043607.01.T01:CDS | 30.0% |
| !! | AAAATTTCTTGGTTTGGTTG+AGG | - | Chr8:27680986-27681005 | MsG0880043607.01.T01:intron | 30.0% |
| !! | GGCTTTAAAATTTCTTGGTT+TGG | - | Chr8:27680980-27680999 | MsG0880043607.01.T01:intron | 30.0% |
| ACCTATCAACATCAAAAGGA+AGG | - | Chr8:27682020-27682039 | MsG0880043607.01.T01:CDS | 35.0% | |
| CCTATCAACATCAAAAGGAA+GGG | - | Chr8:27682021-27682040 | MsG0880043607.01.T01:CDS | 35.0% | |
| CTGTTGAGAAAAATCAAGGT+AGG | - | Chr8:27681207-27681226 | MsG0880043607.01.T01:intron | 35.0% | |
| GTCAATCACCAGATAAAACT+AGG | - | Chr8:27682055-27682074 | MsG0880043607.01.T01:CDS | 35.0% | |
| TATCATCAACAGCCAAAACA+TGG | + | Chr8:27680712-27680731 | None:intergenic | 35.0% | |
| TGTATCAGATGTTTGGAAGA+GGG | - | Chr8:27681901-27681920 | MsG0880043607.01.T01:intron | 35.0% | |
| TTGTATCAGATGTTTGGAAG+AGG | - | Chr8:27681900-27681919 | MsG0880043607.01.T01:intron | 35.0% | |
| ! | AGAGAATCAGTTCCATGTTT+TGG | - | Chr8:27680697-27680716 | MsG0880043607.01.T01:CDS | 35.0% |
| ! | ATGTTCCATCTAGGATCAAT+AGG | - | Chr8:27681735-27681754 | MsG0880043607.01.T01:CDS | 35.0% |
| ! | TTTTCTCAACAGATCATAGC+CGG | + | Chr8:27681199-27681218 | None:intergenic | 35.0% |
| !!! | ACTTTTGGCCTAGTTTTATC+TGG | + | Chr8:27682066-27682085 | None:intergenic | 35.0% |
| CAACATCAAAAGGAAGGGTA+TGG | - | Chr8:27682026-27682045 | MsG0880043607.01.T01:CDS | 40.0% | |
| CTTACATCTGATAGCTGAAC+TGG | + | Chr8:27681949-27681968 | None:intergenic | 40.0% | |
| GTATCAGATGTTTGGAAGAG+GGG | - | Chr8:27681902-27681921 | MsG0880043607.01.T01:intron | 40.0% | |
| GTTTGGTTGAGGATGAAATG+AGG | - | Chr8:27680997-27681016 | MsG0880043607.01.T01:intron | 40.0% | |
| TATCAGATGTTTGGAAGAGG+GGG | - | Chr8:27681903-27681922 | MsG0880043607.01.T01:intron | 40.0% | |
| TCTCACCTATTGATCCTAGA+TGG | + | Chr8:27681743-27681762 | None:intergenic | 40.0% | |
| TCTGAGGACATTATCACAAC+AGG | + | Chr8:27681713-27681732 | None:intergenic | 40.0% | |
| ! | CCCTTCCTTTTGATGTTGAT+AGG | + | Chr8:27682024-27682043 | None:intergenic | 40.0% |
| ! | TACAGTGGATTCTGCTAGTA+AGG | - | Chr8:27680959-27680978 | MsG0880043607.01.T01:intron | 40.0% |
| !! | TGAAGCAATAGGAGTGTCAT+AGG | + | Chr8:27681024-27681043 | None:intergenic | 40.0% |
| CCTAGATGGAACATTCTCTG+AGG | + | Chr8:27681729-27681748 | None:intergenic | 45.0% | |
| CCTCAGAGAATGTTCCATCT+AGG | - | Chr8:27681726-27681745 | MsG0880043607.01.T01:intron | 45.0% | |
| TATTGTATGCCAGGGATGAC+CGG | - | Chr8:27681177-27681196 | MsG0880043607.01.T01:intron | 45.0% | |
| ! | CTGATAGCTGAACTGGTTTC+AGG | + | Chr8:27681942-27681961 | None:intergenic | 45.0% |
| AGATCATAGCCGGTCATCCC+TGG | + | Chr8:27681189-27681208 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 27680689 | 27682095 | 27680689 | ID=MsG0880043607.01;Name=MsG0880043607.01 |
| Chr8 | mRNA | 27680689 | 27682095 | 27680689 | ID=MsG0880043607.01.T01;Parent=MsG0880043607.01;Name=MsG0880043607.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|5|0|177 |
| Chr8 | exon | 27681999 | 27682095 | 27681999 | ID=MsG0880043607.01.T01:exon:8913;Parent=MsG0880043607.01.T01 |
| Chr8 | exon | 27681729 | 27681829 | 27681729 | ID=MsG0880043607.01.T01:exon:8912;Parent=MsG0880043607.01.T01 |
| Chr8 | exon | 27681560 | 27681637 | 27681560 | ID=MsG0880043607.01.T01:exon:8911;Parent=MsG0880043607.01.T01 |
| Chr8 | exon | 27681028 | 27681098 | 27681028 | ID=MsG0880043607.01.T01:exon:8910;Parent=MsG0880043607.01.T01 |
| Chr8 | exon | 27680689 | 27680875 | 27680689 | ID=MsG0880043607.01.T01:exon:8909;Parent=MsG0880043607.01.T01 |
| Chr8 | CDS | 27681999 | 27682095 | 27681999 | ID=MsG0880043607.01.T01:cds;Parent=MsG0880043607.01.T01 |
| Chr8 | CDS | 27681729 | 27681829 | 27681729 | ID=MsG0880043607.01.T01:cds;Parent=MsG0880043607.01.T01 |
| Chr8 | CDS | 27681560 | 27681637 | 27681560 | ID=MsG0880043607.01.T01:cds;Parent=MsG0880043607.01.T01 |
| Chr8 | CDS | 27681028 | 27681098 | 27681028 | ID=MsG0880043607.01.T01:cds;Parent=MsG0880043607.01.T01 |
| Chr8 | CDS | 27680689 | 27680875 | 27680689 | ID=MsG0880043607.01.T01:cds;Parent=MsG0880043607.01.T01 |
| Gene Sequence |
| Protein sequence |
