AlfalfaGEDB | MsG0880047063.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047063.01

KEGG: -
GO: -
PFAM: PF04570

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047063.01.T01	XP_003609698.1	92.105	114	8	1	1	113	1	114	2.50E-62	195
MsG0880047063.01.T02	XP_003609698.1	92.208	77	6	0	1	77	38	114	2.91E-45	150

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047063.01.T01	Q9FH22	62.264	53	20	0	56	108	63	115	4.22E-20	82.4
MsG0880047063.01.T02	Q9FH22	62.264	53	20	0	20	72	63	115	4.49E-20	80.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047063.01.T01	G7JLK0	92.105	114	8	1	1	113	1	114	1.20e-62	195
MsG0880047063.01.T02	G7JLK0	92.208	77	6	0	1	77	38	114	1.39e-45	150

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000777.01	MsG0880047063.01	0.807843	4.145800e-50	2.333583e-47
MsG0180003741.01	MsG0880047063.01	0.810023	1.416258e-50	8.445136e-48
MsG0180004481.01	MsG0880047063.01	0.801164	1.024517e-48	4.859127e-46
MsG0180004765.01	MsG0880047063.01	0.822335	2.503544e-53	2.089388e-50
MsG0180005113.01	MsG0880047063.01	0.806712	7.199360e-50	3.935087e-47
MsG0180005153.01	MsG0880047063.01	0.817610	3.015339e-52	2.206314e-49
MsG0280006360.01	MsG0880047063.01	0.814743	1.318958e-51	8.923008e-49
MsG0280006592.01	MsG0880047063.01	0.847030	1.496007e-59	2.624422e-56
MsG0280007527.01	MsG0880047063.01	0.803895	2.802236e-49	1.424893e-46
MsG0280008899.01	MsG0880047063.01	0.820705	5.955551e-53	4.747259e-50

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047063.01.T01	MTR_4g120160	92.105	114	8	1	1	113	1	114	3.03e-66	195
MsG0880047063.01.T01	MTR_2g436020	59.829	117	34	3	1	109	1	112	9.22e-38	124
MsG0880047063.01.T01	MTR_2g436250	59.829	117	34	3	1	109	1	112	9.22e-38	124
MsG0880047063.01.T01	MTR_3g073960	46.970	66	31	1	38	103	71	132	6.45e-15	66.6
MsG0880047063.01.T01	MTR_8g022950	40.541	74	36	1	36	101	6	79	6.92e-13	60.5
MsG0880047063.01.T01	MTR_4g124930	52.083	48	22	1	56	103	173	219	7.83e-12	60.1
MsG0880047063.01.T01	MTR_4g124930	52.083	48	22	1	56	103	173	219	9.49e-12	59.7
MsG0880047063.01.T01	MTR_3g087700	38.889	72	41	1	35	103	95	166	3.53e-11	57.8
MsG0880047063.01.T01	MTR_3g108290	53.191	47	22	0	56	102	349	395	9.93e-11	57.8
MsG0880047063.01.T02	MTR_4g120160	92.208	77	6	0	1	77	38	114	3.53e-49	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047063.01.T01	AT5G49120	62.264	53	20	0	56	108	63	115	4.30e-21	82.4
MsG0880047063.01.T01	AT1G22160	48.276	58	30	0	53	110	70	127	3.21e-14	64.7
MsG0880047063.01.T01	AT4G39795	42.029	69	39	1	33	101	46	113	4.44e-13	61.2
MsG0880047063.01.T01	AT1G78020	53.488	43	20	0	61	103	89	131	8.65e-13	61.2
MsG0880047063.01.T01	AT5G47060	56.000	50	21	1	61	110	97	145	8.02e-12	58.9
MsG0880047063.01.T01	AT5G47060	56.000	50	21	1	61	110	97	145	8.02e-12	58.9
MsG0880047063.01.T01	AT4G17670	52.000	50	23	1	61	110	76	124	9.87e-11	55.8
MsG0880047063.01.T02	AT5G49120	62.264	53	20	0	20	72	63	115	4.58e-21	80.9
MsG0880047063.01.T02	AT1G22160	48.276	58	30	0	17	74	70	127	2.67e-14	63.5
MsG0880047063.01.T02	AT4G39795	40.580	69	40	1	3	71	52	119	3.84e-13	60.1

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Find 10 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAGAATGATAGGAAAG+TGG	0.494802	8:+81493666	None:intergenic
AACCTTAAAAGGAGAATGAT+AGG	0.405313	8:+81493659	None:intergenic
AGAAAAGTTGAAACCTTAAA+AGG	0.340742	8:+81493648	None:intergenic
ATCAGAGAATGAAATATGGT+TGG	0.543247	8:-81493825	None:intergenic
GAATGAAATATGGTTGGTCT+TGG	0.488935	8:-81493819	None:intergenic
GCAGCAAGAAGCTCTTACCT+GGG	0.606797	8:-81493608	MsG0880047063.01.T01:CDS
GTTGATTGTGCTAAGCATCA+TGG	0.561835	8:+81493704	None:intergenic
TACATGTAGATATCTTTCCC+AGG	0.593576	8:+81493591	None:intergenic
TCTTGCTGCATAGAAAGCAT+TGG	0.559666	8:+81493622	None:intergenic
TGCAGCAAGAAGCTCTTACC+TGG	0.449852	8:-81493609	MsG0880047063.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCTTAAAAGGAGAATGAT+AGG	+	Chr8:81493419-81493438	None:intergenic	30.0%
AAAGGAGAATGATAGGAAAG+TGG	+	Chr8:81493412-81493431	None:intergenic	35.0%
TACATGTACAAGTGAGTTCT+CGG	-	Chr8:81493499-81493518	MsG0880047063.01.T01:intron	35.0%
TACATGTAGATATCTTTCCC+AGG	+	Chr8:81493487-81493506	None:intergenic	35.0%
ATGGCACCTATTTATTGCAG+AGG	-	Chr8:81493716-81493735	MsG0880047063.01.T01:CDS	40.0%
CTGTCTCCTCTGCAATAAAT+AGG	+	Chr8:81493725-81493744	None:intergenic	40.0%
GTTGATTGTGCTAAGCATCA+TGG	+	Chr8:81493374-81493393	None:intergenic	40.0%
CCTACAATCCACGCTACAAA+AGG	+	Chr8:81493750-81493769	None:intergenic	45.0%
GCAGCAAGAAGCTCTTACCT+GGG	-	Chr8:81493467-81493486	MsG0880047063.01.T01:intron	50.0%
TGCAGCAAGAAGCTCTTACC+TGG	-	Chr8:81493466-81493485	MsG0880047063.01.T01:intron	50.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	81493265	81493832	81493265	ID=MsG0880047063.01;Name=MsG0880047063.01
Chr8	mRNA	81493265	81493832	81493265	ID=MsG0880047063.01.T01;Parent=MsG0880047063.01;Name=MsG0880047063.01.T01;_AED=0.30;_eAED=0.31;_QI=0\|0\|0\|1\|0\|0\|2\|0\|113
Chr8	exon	81493588	81493832	81493588	ID=MsG0880047063.01.T01:exon:34918;Parent=MsG0880047063.01.T01
Chr8	CDS	81493588	81493832	81493588	ID=MsG0880047063.01.T01:cds;Parent=MsG0880047063.01.T01
Chr8	CDS	81493265	81493361	81493265	ID=MsG0880047063.01.T01:cds;Parent=MsG0880047063.01.T01
Chr8	mRNA	81493265	81493724	81493265	ID=MsG0880047063.01.T02;Parent=MsG0880047063.01;Name=MsG0880047063.01.T02;_AED=0.36;_eAED=0.37;_QI=0\|0\|0\|1\|0\|0\|2\|0\|77
Chr8	exon	81493588	81493724	81493588	ID=MsG0880047063.01.T02:exon:34920;Parent=MsG0880047063.01.T02
Chr8	CDS	81493588	81493724	81493588	ID=MsG0880047063.01.T02:cds;Parent=MsG0880047063.01.T02
Chr8	CDS	81493265	81493361	81493265	ID=MsG0880047063.01.T02:cds;Parent=MsG0880047063.01.T02

Gene Sequence

>MsG0880047063.01.T01

ATGGTTGGTCTTGGTATTGTGCTTGAAGAACAACAACCACATAAGAAAAAGAGTAATATCAGCATCAACAACAACAATAACACTTGTCAAGTTATTAACAAAACTACCATGATGCTTAGCACAATCAACAACAAAACTTCTTCTCCACTTTCCTATCATTCTCCTTTTAAGGTTTCAACTTTTCTTGACCAATGCTTTCTATGCAGCAAGAAGCTCTTACCTGGGAAAGATATCTACATGTACAAAGGAGACAGAGCCTTTTGTAGCGTGGATTGTAGGTGCAAGCATATTTTAGCGGACGAGGAAGAGGCTATGAAGAAACAGAACATTTTTGAAGAGTAG

>MsG0880047063.01.T02

ATGATGCTTAGCACAATCAACAACAAAACTTCTTCTCCACTTTCCTATCATTCTCCTTTTAAGGTTTCAACTTTTCTTGACCAATGCTTTCTATGCAGCAAGAAGCTCTTACCTGGGAAAGATATCTACATGTACAAAGGAGACAGAGCCTTTTGTAGCGTGGATTGTAGGTGCAAGCATATTTTAGCGGACGAGGAAGAGGCTATGAAGAAACAGAACATTTTTGAAGAGTAG

Protein sequence

>MsG0880047063.01.T01

MVGLGIVLEEQQPHKKKSNISINNNNNTCQVINKTTMMLSTINNKTSSPLSYHSPFKVSTFLDQCFLCSKKLLPGKDIYMYKGDRAFCSVDCRCKHILADEEEAMKKQNIFEE*

>MsG0880047063.01.T02

MMLSTINNKTSSPLSYHSPFKVSTFLDQCFLCSKKLLPGKDIYMYKGDRAFCSVDCRCKHILADEEEAMKKQNIFEE*