Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047063.01.T01 | XP_003609698.1 | 92.105 | 114 | 8 | 1 | 1 | 113 | 1 | 114 | 2.50E-62 | 195 |
| MsG0880047063.01.T02 | XP_003609698.1 | 92.208 | 77 | 6 | 0 | 1 | 77 | 38 | 114 | 2.91E-45 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047063.01.T01 | Q9FH22 | 62.264 | 53 | 20 | 0 | 56 | 108 | 63 | 115 | 4.22E-20 | 82.4 |
| MsG0880047063.01.T02 | Q9FH22 | 62.264 | 53 | 20 | 0 | 20 | 72 | 63 | 115 | 4.49E-20 | 80.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047063.01.T01 | G7JLK0 | 92.105 | 114 | 8 | 1 | 1 | 113 | 1 | 114 | 1.20e-62 | 195 |
| MsG0880047063.01.T02 | G7JLK0 | 92.208 | 77 | 6 | 0 | 1 | 77 | 38 | 114 | 1.39e-45 | 150 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000777.01 | MsG0880047063.01 | 0.807843 | 4.145800e-50 | 2.333583e-47 |
| MsG0880045735.01 | MsG0880047063.01 | 0.825748 | 3.956987e-54 | 3.638911e-51 |
| MsG0880047063.01 | MsG0880047135.01 | 0.832273 | 1.038680e-55 | 1.155119e-52 |
| MsG0880047063.01 | MsG0880047473.01 | 0.801313 | 9.548680e-49 | 4.545859e-46 |
| MsG0880047063.01 | MsG0880047764.01 | 0.825969 | 3.507077e-54 | 3.245537e-51 |
| MsG0180003741.01 | MsG0880047063.01 | 0.810023 | 1.416258e-50 | 8.445136e-48 |
| MsG0180004481.01 | MsG0880047063.01 | 0.801164 | 1.024517e-48 | 4.859127e-46 |
| MsG0180004765.01 | MsG0880047063.01 | 0.822335 | 2.503544e-53 | 2.089388e-50 |
| MsG0180005113.01 | MsG0880047063.01 | 0.806712 | 7.199360e-50 | 3.935087e-47 |
| MsG0180005153.01 | MsG0880047063.01 | 0.817610 | 3.015339e-52 | 2.206314e-49 |
| MsG0280006360.01 | MsG0880047063.01 | 0.814743 | 1.318958e-51 | 8.923008e-49 |
| MsG0780040430.01 | MsG0880047063.01 | 0.831509 | 1.602949e-55 | 1.743229e-52 |
| MsG0780040431.01 | MsG0880047063.01 | 0.816834 | 4.508285e-52 | 3.229285e-49 |
| MsG0780040888.01 | MsG0880047063.01 | 0.809802 | 1.580602e-50 | 9.370464e-48 |
| MsG0780041440.01 | MsG0880047063.01 | 0.818343 | 2.059809e-52 | 1.537907e-49 |
| MsG0880041834.01 | MsG0880047063.01 | 0.831462 | 1.647083e-55 | 1.788681e-52 |
| MsG0280011109.01 | MsG0880047063.01 | 0.817198 | 3.733961e-52 | 2.701409e-49 |
| MsG0280011329.01 | MsG0880047063.01 | 0.819242 | 1.287200e-52 | 9.851652e-50 |
| MsG0380014221.01 | MsG0880047063.01 | 0.846863 | 1.662405e-59 | 2.900725e-56 |
| MsG0680031263.01 | MsG0880047063.01 | 0.850873 | 1.281101e-60 | 2.545470e-57 |
| MsG0680031264.01 | MsG0880047063.01 | 0.837137 | 6.205456e-57 | 7.986354e-54 |
| MsG0680031621.01 | MsG0880047063.01 | 0.856106 | 4.025726e-62 | 9.502972e-59 |
| MsG0680031708.01 | MsG0880047063.01 | 0.800631 | 1.316646e-48 | 6.160800e-46 |
| MsG0680034739.01 | MsG0880047063.01 | 0.810729 | 9.972198e-51 | 6.058211e-48 |
| MsG0680035580.01 | MsG0880047063.01 | 0.816940 | 4.268130e-52 | 3.066360e-49 |
| MsG0480019148.01 | MsG0880047063.01 | 0.814210 | 1.730234e-51 | 1.153760e-48 |
| MsG0480021056.01 | MsG0880047063.01 | 0.818828 | 1.598688e-52 | 1.209642e-49 |
| MsG0480022054.01 | MsG0880047063.01 | 0.809998 | 1.433859e-50 | 8.544517e-48 |
| MsG0480022711.01 | MsG0880047063.01 | 0.803044 | 4.205331e-49 | 2.091966e-46 |
| MsG0480023055.01 | MsG0880047063.01 | 0.825215 | 5.293836e-54 | 4.794915e-51 |
| MsG0580024197.01 | MsG0880047063.01 | 0.802153 | 6.420418e-49 | 3.122321e-46 |
| MsG0580024952.01 | MsG0880047063.01 | 0.828397 | 9.198332e-55 | 9.132261e-52 |
| MsG0580025053.01 | MsG0880047063.01 | 0.808239 | 3.415085e-50 | 1.942549e-47 |
| MsG0580026086.01 | MsG0880047063.01 | 0.808304 | 3.308192e-50 | 1.884898e-47 |
| MsG0580027655.01 | MsG0880047063.01 | 0.814247 | 1.697475e-51 | 1.133128e-48 |
| MsG0580028407.01 | MsG0880047063.01 | 0.831038 | 2.092957e-55 | 2.244376e-52 |
| MsG0580029736.01 | MsG0880047063.01 | 0.818313 | 2.092656e-52 | 1.561021e-49 |
| MsG0280006592.01 | MsG0880047063.01 | 0.847030 | 1.496007e-59 | 2.624422e-56 |
| MsG0280007527.01 | MsG0880047063.01 | 0.803895 | 2.802236e-49 | 1.424893e-46 |
| MsG0280008899.01 | MsG0880047063.01 | 0.820705 | 5.955551e-53 | 4.747259e-50 |
| MsG0280010184.01 | MsG0880047063.01 | 0.815351 | 9.666845e-52 | 6.648915e-49 |
| MsG0780036766.01 | MsG0880047063.01 | 0.800565 | 1.357820e-48 | 6.342717e-46 |
| MsG0780038907.01 | MsG0880047063.01 | 0.811888 | 5.589554e-51 | 3.501197e-48 |
| MsG0780039940.01 | MsG0880047063.01 | 0.852769 | 3.714245e-61 | 7.844609e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047063.01.T01 | MTR_4g120160 | 92.105 | 114 | 8 | 1 | 1 | 113 | 1 | 114 | 3.03e-66 | 195 |
| MsG0880047063.01.T01 | MTR_2g436020 | 59.829 | 117 | 34 | 3 | 1 | 109 | 1 | 112 | 9.22e-38 | 124 |
| MsG0880047063.01.T01 | MTR_2g436250 | 59.829 | 117 | 34 | 3 | 1 | 109 | 1 | 112 | 9.22e-38 | 124 |
| MsG0880047063.01.T01 | MTR_3g073960 | 46.970 | 66 | 31 | 1 | 38 | 103 | 71 | 132 | 6.45e-15 | 66.6 |
| MsG0880047063.01.T01 | MTR_8g022950 | 40.541 | 74 | 36 | 1 | 36 | 101 | 6 | 79 | 6.92e-13 | 60.5 |
| MsG0880047063.01.T01 | MTR_4g124930 | 52.083 | 48 | 22 | 1 | 56 | 103 | 173 | 219 | 7.83e-12 | 60.1 |
| MsG0880047063.01.T01 | MTR_4g124930 | 52.083 | 48 | 22 | 1 | 56 | 103 | 173 | 219 | 9.49e-12 | 59.7 |
| MsG0880047063.01.T01 | MTR_3g087700 | 38.889 | 72 | 41 | 1 | 35 | 103 | 95 | 166 | 3.53e-11 | 57.8 |
| MsG0880047063.01.T01 | MTR_3g108290 | 53.191 | 47 | 22 | 0 | 56 | 102 | 349 | 395 | 9.93e-11 | 57.8 |
| MsG0880047063.01.T02 | MTR_4g120160 | 92.208 | 77 | 6 | 0 | 1 | 77 | 38 | 114 | 3.53e-49 | 150 |
| MsG0880047063.01.T02 | MTR_2g436020 | 65.432 | 81 | 20 | 1 | 1 | 73 | 32 | 112 | 2.05e-31 | 107 |
| MsG0880047063.01.T02 | MTR_2g436250 | 65.432 | 81 | 20 | 1 | 1 | 73 | 32 | 112 | 2.05e-31 | 107 |
| MsG0880047063.01.T02 | MTR_3g073960 | 46.970 | 66 | 31 | 1 | 2 | 67 | 71 | 132 | 2.94e-15 | 66.2 |
| MsG0880047063.01.T02 | MTR_8g022950 | 41.096 | 73 | 35 | 1 | 1 | 65 | 7 | 79 | 1.24e-13 | 61.2 |
| MsG0880047063.01.T02 | MTR_4g124930 | 52.083 | 48 | 22 | 1 | 20 | 67 | 173 | 219 | 3.12e-12 | 59.7 |
| MsG0880047063.01.T02 | MTR_4g124930 | 52.083 | 48 | 22 | 1 | 20 | 67 | 173 | 219 | 3.61e-12 | 59.7 |
| MsG0880047063.01.T02 | MTR_3g087700 | 53.488 | 43 | 20 | 0 | 25 | 67 | 124 | 166 | 2.78e-11 | 56.6 |
| MsG0880047063.01.T02 | MTR_3g108290 | 53.191 | 47 | 22 | 0 | 20 | 66 | 349 | 395 | 9.11e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047063.01.T01 | AT5G49120 | 62.264 | 53 | 20 | 0 | 56 | 108 | 63 | 115 | 4.30e-21 | 82.4 |
| MsG0880047063.01.T01 | AT1G22160 | 48.276 | 58 | 30 | 0 | 53 | 110 | 70 | 127 | 3.21e-14 | 64.7 |
| MsG0880047063.01.T01 | AT4G39795 | 42.029 | 69 | 39 | 1 | 33 | 101 | 46 | 113 | 4.44e-13 | 61.2 |
| MsG0880047063.01.T01 | AT1G78020 | 53.488 | 43 | 20 | 0 | 61 | 103 | 89 | 131 | 8.65e-13 | 61.2 |
| MsG0880047063.01.T01 | AT5G47060 | 56.000 | 50 | 21 | 1 | 61 | 110 | 97 | 145 | 8.02e-12 | 58.9 |
| MsG0880047063.01.T01 | AT5G47060 | 56.000 | 50 | 21 | 1 | 61 | 110 | 97 | 145 | 8.02e-12 | 58.9 |
| MsG0880047063.01.T01 | AT4G17670 | 52.000 | 50 | 23 | 1 | 61 | 110 | 76 | 124 | 9.87e-11 | 55.8 |
| MsG0880047063.01.T02 | AT5G49120 | 62.264 | 53 | 20 | 0 | 20 | 72 | 63 | 115 | 4.58e-21 | 80.9 |
| MsG0880047063.01.T02 | AT1G22160 | 48.276 | 58 | 30 | 0 | 17 | 74 | 70 | 127 | 2.67e-14 | 63.5 |
| MsG0880047063.01.T02 | AT4G39795 | 40.580 | 69 | 40 | 1 | 3 | 71 | 52 | 119 | 3.84e-13 | 60.1 |
| MsG0880047063.01.T02 | AT1G78020 | 51.064 | 47 | 23 | 0 | 25 | 71 | 89 | 135 | 7.60e-13 | 60.1 |
| MsG0880047063.01.T02 | AT5G47060 | 56.000 | 50 | 21 | 1 | 25 | 74 | 97 | 145 | 3.87e-12 | 58.5 |
| MsG0880047063.01.T02 | AT5G47060 | 56.000 | 50 | 21 | 1 | 25 | 74 | 97 | 145 | 3.87e-12 | 58.5 |
| MsG0880047063.01.T02 | AT5G20700 | 45.455 | 66 | 32 | 3 | 3 | 67 | 161 | 223 | 5.18e-11 | 56.2 |
| MsG0880047063.01.T02 | AT4G17670 | 52.000 | 50 | 23 | 1 | 25 | 74 | 76 | 124 | 5.32e-11 | 55.1 |
Find 10 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAAGTTGAAACCTTAAA+AGG | 0.340742 | 8:+81493648 | None:intergenic |
| AACCTTAAAAGGAGAATGAT+AGG | 0.405313 | 8:+81493659 | None:intergenic |
| TGCAGCAAGAAGCTCTTACC+TGG | 0.449852 | 8:-81493609 | MsG0880047063.01.T01:CDS |
| GAATGAAATATGGTTGGTCT+TGG | 0.488935 | 8:-81493819 | None:intergenic |
| AAAGGAGAATGATAGGAAAG+TGG | 0.494802 | 8:+81493666 | None:intergenic |
| ATCAGAGAATGAAATATGGT+TGG | 0.543247 | 8:-81493825 | None:intergenic |
| TCTTGCTGCATAGAAAGCAT+TGG | 0.559666 | 8:+81493622 | None:intergenic |
| GTTGATTGTGCTAAGCATCA+TGG | 0.561835 | 8:+81493704 | None:intergenic |
| TACATGTAGATATCTTTCCC+AGG | 0.593576 | 8:+81493591 | None:intergenic |
| GCAGCAAGAAGCTCTTACCT+GGG | 0.606797 | 8:-81493608 | MsG0880047063.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAAGATCTATATATACTAT+AGG | + | Chr8:81493585-81493604 | None:intergenic | 20.0% |
| !!! | ATATTACTCTTTTTCTTATG+TGG | + | Chr8:81493304-81493323 | None:intergenic | 20.0% |
| !!! | ATGCATTTTTTTTTTCTTCT+TGG | - | Chr8:81493606-81493625 | MsG0880047063.01.T01:CDS | 20.0% |
| !!! | GAATTTTGTATTAGTATATG+TGG | - | Chr8:81493675-81493694 | MsG0880047063.01.T01:CDS | 20.0% |
| !!! | GAGTTATTTTTTTGTCTTAA+TGG | - | Chr8:81493697-81493716 | MsG0880047063.01.T01:CDS | 20.0% |
| ! | AGAAAAGTTGAAACCTTAAA+AGG | + | Chr8:81493430-81493449 | None:intergenic | 25.0% |
| ! | CAATTAACATCAAAAGATCT+GGG | + | Chr8:81493656-81493675 | None:intergenic | 25.0% |
| ! | TCAATTAACATCAAAAGATC+TGG | + | Chr8:81493657-81493676 | None:intergenic | 25.0% |
| AACCTTAAAAGGAGAATGAT+AGG | + | Chr8:81493419-81493438 | None:intergenic | 30.0% | |
| ! | AATGCATGCTACACTTATTT+TGG | + | Chr8:81493527-81493546 | None:intergenic | 30.0% |
| ! | TTCCTATCATTCTCCTTTTA+AGG | - | Chr8:81493414-81493433 | MsG0880047063.01.T01:intron | 30.0% |
| !!! | ATCTGGGTATTTTTTCTGTA+AGG | + | Chr8:81493640-81493659 | None:intergenic | 30.0% |
| AAAGGAGAATGATAGGAAAG+TGG | + | Chr8:81493412-81493431 | None:intergenic | 35.0% | |
| TACATGTACAAGTGAGTTCT+CGG | - | Chr8:81493499-81493518 | MsG0880047063.01.T01:intron | 35.0% | |
| TACATGTAGATATCTTTCCC+AGG | + | Chr8:81493487-81493506 | None:intergenic | 35.0% | |
| !! | TAGGTGCAAGCATATTTTAG+CGG | - | Chr8:81493766-81493785 | MsG0880047063.01.T01:CDS | 35.0% |
| ATGGCACCTATTTATTGCAG+AGG | - | Chr8:81493716-81493735 | MsG0880047063.01.T01:CDS | 40.0% | |
| CTGTCTCCTCTGCAATAAAT+AGG | + | Chr8:81493725-81493744 | None:intergenic | 40.0% | |
| GTTGATTGTGCTAAGCATCA+TGG | + | Chr8:81493374-81493393 | None:intergenic | 40.0% | |
| ! | TCTTGCTGCATAGAAAGCAT+TGG | + | Chr8:81493456-81493475 | None:intergenic | 40.0% |
| CCTACAATCCACGCTACAAA+AGG | + | Chr8:81493750-81493769 | None:intergenic | 45.0% | |
| !! | CCTTTTGTAGCGTGGATTGT+AGG | - | Chr8:81493747-81493766 | MsG0880047063.01.T01:CDS | 45.0% |
| !!! | CAAGCATATTTTAGCGGACG+AGG | - | Chr8:81493772-81493791 | MsG0880047063.01.T01:CDS | 45.0% |
| !!! | TATTTTAGCGGACGAGGAAG+AGG | - | Chr8:81493778-81493797 | MsG0880047063.01.T01:CDS | 45.0% |
| GCAGCAAGAAGCTCTTACCT+GGG | - | Chr8:81493467-81493486 | MsG0880047063.01.T01:intron | 50.0% | |
| TGCAGCAAGAAGCTCTTACC+TGG | - | Chr8:81493466-81493485 | MsG0880047063.01.T01:intron | 50.0% | |
| ! | AGACAGAGCCTTTTGTAGCG+TGG | - | Chr8:81493739-81493758 | MsG0880047063.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 81493265 | 81493832 | 81493265 | ID=MsG0880047063.01;Name=MsG0880047063.01 |
| Chr8 | mRNA | 81493265 | 81493832 | 81493265 | ID=MsG0880047063.01.T01;Parent=MsG0880047063.01;Name=MsG0880047063.01.T01;_AED=0.30;_eAED=0.31;_QI=0|0|0|1|0|0|2|0|113 |
| Chr8 | exon | 81493588 | 81493832 | 81493588 | ID=MsG0880047063.01.T01:exon:34918;Parent=MsG0880047063.01.T01 |
| Chr8 | CDS | 81493588 | 81493832 | 81493588 | ID=MsG0880047063.01.T01:cds;Parent=MsG0880047063.01.T01 |
| Chr8 | CDS | 81493265 | 81493361 | 81493265 | ID=MsG0880047063.01.T01:cds;Parent=MsG0880047063.01.T01 |
| Chr8 | mRNA | 81493265 | 81493724 | 81493265 | ID=MsG0880047063.01.T02;Parent=MsG0880047063.01;Name=MsG0880047063.01.T02;_AED=0.36;_eAED=0.37;_QI=0|0|0|1|0|0|2|0|77 |
| Chr8 | exon | 81493588 | 81493724 | 81493588 | ID=MsG0880047063.01.T02:exon:34920;Parent=MsG0880047063.01.T02 |
| Chr8 | CDS | 81493588 | 81493724 | 81493588 | ID=MsG0880047063.01.T02:cds;Parent=MsG0880047063.01.T02 |
| Chr8 | CDS | 81493265 | 81493361 | 81493265 | ID=MsG0880047063.01.T02:cds;Parent=MsG0880047063.01.T02 |
| Gene Sequence |
| Protein sequence |
