AlfalfaGEDB | Msa1424580

M. sativa cultivar zhongmu-4 / Msa1424580

KEGG: -
GO: -
PFAM: PF05278.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424580	G7J8J7	90.051	392	19	5	3	387	7	385	0.0	644

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001380	Msa1424580	0.917825	3.308167e-86	-8.615850e-47
Msa0013280	Msa1424580	0.849504	3.098857e-60	-8.615850e-47
Msa0064140	Msa1424580	0.813358	2.664712e-51	-8.615850e-47
Msa0071470	Msa1424580	0.810580	1.073981e-50	-8.615850e-47
Msa0091930	Msa1424580	0.803342	3.649460e-49	-8.615850e-47
Msa0104810	Msa1424580	0.802322	5.926664e-49	-8.615850e-47
Msa0106530	Msa1424580	0.852036	6.007237e-61	-8.615850e-47
Msa0119430	Msa1424580	0.825512	4.502701e-54	-8.615850e-47
Msa0187310	Msa1424580	0.827647	1.393851e-54	-8.615850e-47
Msa0224130	Msa1424580	0.814443	1.536742e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424580	MtrunA17_Chr3g0145681	90.051	392	19	5	3	387	7	385	0.0	644
Msa1424580	MtrunA17_Chr1g0211071	48.227	423	161	16	5	386	15	420	9.12e-106	318
Msa1424580	MtrunA17_Chr8g0381111	29.213	178	110	6	154	324	253	421	6.49e-11	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424580	AT4G35110.1	31.514	403	226	15	5	387	14	386	3.04e-38	142
Msa1424580	AT4G35110.2	31.514	403	226	15	5	387	14	386	3.04e-38	142
Msa1424580	AT4G35110.6	31.514	403	226	15	5	387	14	386	3.04e-38	142
Msa1424580	AT4G35110.5	31.514	403	226	15	5	387	14	386	3.04e-38	142
Msa1424580	AT4G35110.4	31.514	403	226	15	5	387	26	398	5.81e-38	141
Msa1424580	AT4G35110.3	31.514	403	226	15	5	387	29	401	6.46e-38	141
Msa1424580	AT2G16900.5	39.521	167	94	2	198	363	196	356	5.85e-34	130
Msa1424580	AT2G16900.6	39.521	167	94	2	198	363	196	356	5.85e-34	130
Msa1424580	AT2G16900.1	39.521	167	94	2	198	363	196	356	5.85e-34	130
Msa1424580	AT2G16900.3	39.521	167	94	2	198	363	107	267	6.99e-34	127

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Find 94 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCACATCATGTTGCTGC+TGG	0.525538	tig0025770:+31527	Msa1424580:CDS
AAAGTATTTGGTCGATCTGT+AGG	0.544091	tig0025770:+33355	Msa1424580:three_prime_UTR
AAAGTCACAACTCATGACGT+TGG	0.643038	tig0025770:-33530	None:intergenic
AAATTGGATGCAAGCAACAT+AGG	0.517141	tig0025770:+33309	Msa1424580:three_prime_UTR
AAGATCAATATGTCATTAAT+TGG	0.195712	tig0025770:+33492	Msa1424580:three_prime_UTR
AAGGAAACTGCTTATCACAT+TGG	0.588505	tig0025770:+33227	Msa1424580:three_prime_UTR
AAGTAATAGCAATAATAGAA+AGG	0.561570	tig0025770:+31556	Msa1424580:CDS
AATAATAGAAAGGTTATTAA+TGG	0.347675	tig0025770:+31566	Msa1424580:CDS
AATACATTACTCAATAAAAT+TGG	0.312289	tig0025770:+33293	Msa1424580:three_prime_UTR
AATAGAAAGGTTATTAATGG+AGG	0.564289	tig0025770:+31569	Msa1424580:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAATTGAGAAAGATTGAAG+GGG	-	tig0025770:31424-31443	None:intergenic	30.0%
TGATAAGGATTGGATGATTT+AGG	-	tig0025770:31623-31642	None:intergenic	30.0%
ATCACACTTATGATAAGGAT+TGG	-	tig0025770:31633-31652	None:intergenic	30.0%
GTTTGACCATTTCATTAGAA+TGG	+	tig0025770:31958-31977	Msa1424580:intron	30.0%
ACCATTTCATTAGAATGGAT+TGG	+	tig0025770:31963-31982	Msa1424580:intron	30.0%
ACCAATCCATTCTAATGAAA+TGG	-	tig0025770:31967-31986	None:intergenic	30.0%
TGTGTAATTGAGCAGAAATT+GGG	-	tig0025770:32262-32281	None:intergenic	30.0%
ATGTGTAATTGAGCAGAAAT+TGG	-	tig0025770:32263-32282	None:intergenic	30.0%
CAAACAATACTTGACAAGTA+TGG	+	tig0025770:32681-32700	Msa1424580:CDS	30.0%
TTGACAAGTATGGTGATATA+GGG	+	tig0025770:32691-32710	Msa1424580:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0025770	gene	31408	33591	31408	ID=Msa1424580;Name=Msa1424580
tig0025770	mRNA	31408	33591	31408	ID=Msa1424580-mRNA-1;Parent=Msa1424580;Name=Msa1424580-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0.66\|0.25\|0.75\|0.66\|0.5\|4\|422\|387
tig0025770	exon	31408	31414	31408	ID=Msa1424580-mRNA-1:exon:3835;Parent=Msa1424580-mRNA-1
tig0025770	exon	31465	31671	31465	ID=Msa1424580-mRNA-1:exon:3836;Parent=Msa1424580-mRNA-1
tig0025770	exon	32123	32295	32123	ID=Msa1424580-mRNA-1:exon:3837;Parent=Msa1424580-mRNA-1
tig0025770	exon	32393	33591	32393	ID=Msa1424580-mRNA-1:exon:3838;Parent=Msa1424580-mRNA-1
tig0025770	CDS	31408	31414	31408	ID=Msa1424580-mRNA-1:cds;Parent=Msa1424580-mRNA-1
tig0025770	CDS	31465	31671	31465	ID=Msa1424580-mRNA-1:cds;Parent=Msa1424580-mRNA-1
tig0025770	CDS	32123	32295	32123	ID=Msa1424580-mRNA-1:cds;Parent=Msa1424580-mRNA-1
tig0025770	CDS	32393	33169	32393	ID=Msa1424580-mRNA-1:cds;Parent=Msa1424580-mRNA-1
tig0025770	three_prime_UTR	33170	33591	33170	ID=Msa1424580-mRNA-1:three_prime_utr;Parent=Msa1424580-mRNA-1

Gene Sequence

>Msa1424580

ATGGACGCAAATGCTTCAAATCCTTACCATCAATGCACTCAAGCTTGCTCCCAAAAGACAAAGGGAACCAAACCACATCATGTTGCTGCTGGTACTGCAAGTAATAGCAATAATAGAAAGGTTATTAATGGAGGTGAAAGACGTACTTATGCTTCTTCTTGTCCTAAATCATCCAATCCTTATCATAAGTGTGATGCCAATTGCAACAATTCAGGTGCCACTCCTCATTCCAAGATTGATCATAGGAAGAAGGTGGGTTCTAAGCCACAACCTCCTGTTCTTCAAAGTGTTCCACCAACAAAACCACATTATTCTCATAACAAAAACGATGAGATCATTCCTTCTTCTGGCCCAATTTCTGCTCAATTACACATCCCAGATGTTATGCCTAAAGATCAAGTTAAGGATGGTGCTACTGAGGTCCTCCCCAATAAAACTGAAACAAAGAAAGAGGACAAAGTCGCTGCTTCTCATGAAATCGTTCCAGTAACCAATTCTGATGAAACTGGTGAAGGTGGTTCCAAAGATTTTAGTTTCTCTGGCAATCCCCTTCCTCTCCACAACAAAGAAATAGATACTAGTAGCGAAGGAGAAGCAGATAGTGTCAGTGTTGTATCTGAATCCCGTGTTTCAATTGGAAAATACAATGTCAAAGAAAGTTTTGGTTCCATCCTACAAACAATACTTGACAAGTATGGTGATATAGGGGCAAGCTGTGATTTGGAATCAGTTGTAATGAGGTCATATTACATGGAGTGTGTATGCTTTGTGGTCCAAGAATTACAATCTTCTTCCGATTCAATAAGTAAGACCAAAGTGAGTGAATTGTTGGATATTGTTAAGGATGTTGAATCTGCTCATCTTCGTGTAGCATGGTTGCGTAACACACTCGATGAAATTGTTGAAAATATTGAATTGATTAGTCACCATCAGGATATGGAAATGGAAAAGGCTAACTATGATCGTGAAATGGAATCATTGAGAGAACAGCTAGAATCAGAGTTGGAAACATTGGCTCAGAAAGAACAAGAGGTTGCTGATATTAATATCCGAATTCCTGAAATCAGAGATCGTTTGAGCGAGCTTGAGCAGCTCATGTCTTCAGGATTAGTAGATGATCAAACCACGCTACCTATCAAGTCAAAGATAGATCAACTATTATAA

Protein sequence

>Msa1424580

MDANASNPYHQCTQACSQKTKGTKPHHVAAGTASNSNNRKVINGGERRTYASSCPKSSNPYHKCDANCNNSGATPHSKIDHRKKVGSKPQPPVLQSVPPTKPHYSHNKNDEIIPSSGPISAQLHIPDVMPKDQVKDGATEVLPNKTETKKEDKVAASHEIVPVTNSDETGEGGSKDFSFSGNPLPLHNKEIDTSSEGEADSVSVVSESRVSIGKYNVKESFGSILQTILDKYGDIGASCDLESVVMRSYYMECVCFVVQELQSSSDSISKTKVSELLDIVKDVESAHLRVAWLRNTLDEIVENIELISHHQDMEMEKANYDREMESLREQLESELETLAQKEQEVADINIRIPEIRDRLSELEQLMSSGLVDDQTTLPIKSKIDQLL*