AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0104740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0104740 MtrunA17_Chr1g0159321 97.179 390 9 1 1 388 1 390 0.0 790
Msa0104740 MtrunA17_Chr3g0126011 75.291 344 82 1 45 388 26 366 0.0 551
Msa0104740 MtrunA17_Chr3g0125981 71.745 361 92 2 26 386 18 368 0.0 539
Msa0104740 MtrunA17_Chr3g0125991 71.788 358 96 2 33 388 12 366 0.0 538
Msa0104740 MtrunA17_Chr3g0125781 71.429 350 97 1 39 388 23 369 0.0 534
Msa0104740 MtrunA17_Chr5g0406961 70.487 349 99 2 40 388 100 444 0.0 530
Msa0104740 MtrunA17_Chr4g0045661 70.487 349 99 2 40 388 49 393 0.0 528
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0104740 AT1G43800.1 77.654 358 76 3 33 386 32 389 0.0 587
Msa0104740 AT3G02630.1 69.855 345 101 2 42 386 53 394 0.0 520
Msa0104740 AT2G43710.2 69.054 349 104 2 40 388 57 401 0.0 515
Msa0104740 AT2G43710.1 69.054 349 104 2 40 388 57 401 0.0 515
Msa0104740 AT5G16240.1 67.246 345 110 2 42 386 51 392 6.36e-178 501
Msa0104740 AT5G16230.1 67.353 340 109 2 50 388 63 401 5.36e-174 491
Msa0104740 AT3G02610.2 68.222 343 103 4 50 388 73 413 5.07e-173 489
Msa0104740 AT3G02610.1 68.222 343 103 4 50 388 71 411 5.95e-173 489
Msa0104740 AT3G02620.1 63.904 374 119 7 26 388 35 403 1.72e-170 482
Msa0104740 AT3G02620.2 63.904 374 119 7 26 388 38 406 1.97e-170 482
Msa0104740 AT5G16230.2 66.871 326 106 2 64 388 1 325 1.60e-165 466
Msa0104740 AT3G02620.3 66.869 329 103 4 64 388 1 327 1.99e-162 459

Find 117 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGACTTATTTGTATCTTTC+TGG 0.182339 1_3:+12890385 Msa0104740:CDS
CAATGATTACGCTGATATTT+TGG 0.196546 1_3:+12891789 Msa0104740:CDS
AAAACTGTTCAGTATCTTAT+TGG 0.203529 1_3:+12890433 Msa0104740:CDS
GTGTATACAAATCCCAAGTA+TGG 0.240289 1_3:-12891460 None:intergenic
GATTAACGGGTGAGGGAAAA+AGG 0.240677 1_3:+12891851 Msa0104740:CDS
TGTTGTGTTGGTTGGTGATA+TGG 0.248245 1_3:+12890219 Msa0104740:CDS
GTGGTGGTGATGGTGGATTT+TGG 0.262583 1_3:-12889965 None:intergenic
TTTGGAATGTTCCATGTTAA+TGG 0.266021 1_3:-12889902 None:intergenic
TGGTGGCATTGAGTGGGTTT+TGG 0.304471 1_3:-12890008 None:intergenic
AGTTTGAATATGCATTGTTT+TGG 0.317713 1_3:-12889858 None:intergenic
CTCTAATGTTGGAGAGGATA+TGG 0.327487 1_3:+12892157 Msa0104740:three_prime_UTR
GTCCAAGCCCGTGGGCCGTT+TGG 0.332806 1_3:+12890317 Msa0104740:CDS
TCTTTCTGGAAGGGTTGATA+TGG 0.335225 1_3:+12890399 Msa0104740:CDS
GACACTAAACAAGGAACTTT+GGG 0.344459 1_3:+12892083 Msa0104740:three_prime_UTR
GTCCAAACGGCCCACGGGCT+TGG 0.353727 1_3:-12890319 None:intergenic
GCGGTGTGGTCGGTGGTTGA+TGG 0.380740 1_3:-12889927 None:intergenic
GGCTGCCAGCATTGTTCCAC+TGG 0.386228 1_3:-12890097 None:intergenic
TGACACTAAACAAGGAACTT+TGG 0.388024 1_3:+12892082 Msa0104740:three_prime_UTR
AACGGCCCACGGGCTTGGAC+TGG 0.398332 1_3:-12890314 None:intergenic
CACATGGGGTCAAGTTTAGT+TGG 0.400607 1_3:+12891956 Msa0104740:CDS
TTTAATCGAACGGTGGAGAT+TGG 0.401193 1_3:+12891816 Msa0104740:CDS
ATACAAATGTTGCTCGCTCT+TGG 0.405320 1_3:-12891489 None:intergenic
GACAGAAAACAACCCATACT+TGG 0.410965 1_3:+12891447 Msa0104740:CDS
GGTTAGCACCTAGGATTAGA+AGG 0.411387 1_3:+12891893 Msa0104740:CDS
ATTTGATGAATTTACTGATC+AGG 0.419139 1_3:+12890150 Msa0104740:CDS
TCAAATGGCAATGAAGGATC+AGG 0.420107 1_3:-12890133 None:intergenic
CCACATATGCGCGCAAGCAC+TGG 0.427060 1_3:-12891553 None:intergenic
GCGAAGTGCTCGAATAGCTT+AGG 0.427800 1_3:-12891733 None:intergenic
CAAAATATCAGCGTAATCAT+TGG 0.433099 1_3:-12891788 None:intergenic
GTAATAGGTGGCGGTGTGGT+CGG 0.450987 1_3:-12889937 None:intergenic
GAAAAGCTTGAAGGATTAAC+GGG 0.453295 1_3:+12891838 Msa0104740:CDS
TTTGCATTGGACAGGACCCC+GGG 0.454311 1_3:+12891425 Msa0104740:intron
CGACGGTGGTGTGGTGGTGA+TGG 0.457737 1_3:-12889975 None:intergenic
TGATCAGTAAATTCATCAAA+TGG 0.459663 1_3:-12890148 None:intergenic
ATAGACATGGGGATTTGCTT+AGG 0.460553 1_3:+12890365 Msa0104740:CDS
GGAAAAGCTTGAAGGATTAA+CGG 0.464892 1_3:+12891837 Msa0104740:CDS
GGGCCGTTTGGACACGAGCT+TGG 0.467549 1_3:+12890329 Msa0104740:CDS
ATTCGAGCACTTCGCGGCCG+TGG 0.474737 1_3:+12891741 Msa0104740:CDS
GTTCAGTATCTTATTGGAGC+TGG 0.477043 1_3:+12890439 Msa0104740:CDS
AATTCATCAAATGGCAATGA+AGG 0.478762 1_3:-12890139 None:intergenic
CGAAGTGCTCGAATAGCTTA+GGG 0.481594 1_3:-12891732 None:intergenic
TTGTGTGTGGGTTAGCACCT+AGG 0.482074 1_3:+12891884 Msa0104740:CDS
AACAAGGAACTTTGGGTGAT+GGG 0.486012 1_3:+12892090 Msa0104740:three_prime_UTR
TCTTGCAACCTTCTAATCCT+AGG 0.486803 1_3:-12891901 None:intergenic
AATGGCTAGCATTGCTCCTG+TGG 0.488560 1_3:-12891650 None:intergenic
AACACAACAAAATACTCATC+AGG 0.489365 1_3:-12890205 None:intergenic
CCGGCACACCTTATGTATGA+TGG 0.493314 1_3:+12891703 Msa0104740:CDS
GGTCTTGTCCATCATACATA+AGG 0.494290 1_3:-12891711 None:intergenic
AGACTAGAGATTAAGAGCTA+AGG 0.494866 1_3:+12892034 Msa0104740:three_prime_UTR
TTATTTGTATCTTTCTGGAA+GGG 0.495330 1_3:+12890390 Msa0104740:CDS
GACTAGAGATTAAGAGCTAA+GGG 0.495595 1_3:+12892035 Msa0104740:three_prime_UTR
CGGTGGTGTGGTGGTGATGG+TGG 0.503987 1_3:-12889972 None:intergenic
GATGGTGTTGGTGATGAGGT+TGG 0.504177 1_3:+12890289 Msa0104740:CDS
GCGGCCGTGGCACAGCGCAC+CGG 0.504431 1_3:+12891754 Msa0104740:CDS
TGGAGATTGGAAAAGCTTGA+AGG 0.504527 1_3:+12891829 Msa0104740:CDS
CCAGTGCTTGCGCGCATATG+TGG 0.504826 1_3:+12891553 Msa0104740:CDS
ATGGCTAGCATTGCTCCTGT+GGG 0.506752 1_3:-12891649 None:intergenic
GGAACTTTGGGTGATGGGAG+TGG 0.519583 1_3:+12892095 Msa0104740:three_prime_UTR
AACTCCGGTGCGCTGTGCCA+CGG 0.520106 1_3:-12891758 None:intergenic
TCTTGATGGTGTTGGTGATG+AGG 0.523044 1_3:+12890285 Msa0104740:CDS
TAAAGCCAGTGGAACAATGC+TGG 0.530542 1_3:+12890092 Msa0104740:CDS
GCTCGTGTCCAAACGGCCCA+CGG 0.531927 1_3:-12890325 None:intergenic
TTTCTCCATCATATCACCAA+TGG 0.532359 1_3:-12891668 None:intergenic
GTATCTTATTGGAGCTGGCA+TGG 0.533582 1_3:+12890444 Msa0104740:CDS
TTGGTTCCAGTCCAAGCCCG+TGG 0.534499 1_3:+12890308 Msa0104740:CDS
TTGGTGGTGGTAATAGGTGG+CGG 0.534962 1_3:-12889946 None:intergenic
TAAGCTATTCGAGCACTTCG+CGG 0.535770 1_3:+12891735 Msa0104740:CDS
AGTCCAAGCTCGTGTCCAAA+CGG 0.536753 1_3:-12890332 None:intergenic
GTTTGCATTGGACAGGACCC+CGG 0.538725 1_3:+12891424 Msa0104740:intron
GGATTAGAAGGTTGCAAGAG+AGG 0.539517 1_3:+12891905 Msa0104740:CDS
AGTATGATCAATGGTCTTGA+TGG 0.539669 1_3:+12890271 Msa0104740:CDS
GCTAGCCATTGGTGATATGA+TGG 0.539965 1_3:+12891663 Msa0104740:CDS
CTTATTTGTATCTTTCTGGA+AGG 0.544191 1_3:+12890389 Msa0104740:CDS
CCATCATACATAAGGTGTGC+CGG 0.547215 1_3:-12891703 None:intergenic
ATAGGTGGCGGTGTGGTCGG+TGG 0.548279 1_3:-12889934 None:intergenic
AAACAAGGAACTTTGGGTGA+TGG 0.549981 1_3:+12892089 Msa0104740:three_prime_UTR
TTTCAAATCACTAGAAAACT+GGG 0.554024 1_3:+12890045 Msa0104740:CDS
CGTCGAGAAGCTTCTAGAAG+TGG 0.554197 1_3:+12891624 Msa0104740:CDS
TGTTCCACTGGCTTTAGTAG+TGG 0.556461 1_3:-12890085 None:intergenic
CTCGTGTCCAAACGGCCCAC+GGG 0.556802 1_3:-12890324 None:intergenic
ACGGCTCGCTTAGCGAAAGA+AGG 0.562132 1_3:+12891523 Msa0104740:CDS
ACAGGAGCAATGCTAGCCAT+TGG 0.563098 1_3:+12891652 Msa0104740:CDS
CTTGAAGGATTAACGGGTGA+GGG 0.586052 1_3:+12891844 Msa0104740:CDS
TGTATACAAATCCCAAGTAT+GGG 0.587703 1_3:-12891459 None:intergenic
ACGTATCAGAGTATGATCAA+TGG 0.591704 1_3:+12890262 Msa0104740:CDS
ACAGCGCACCGGAGTTTACA+CGG 0.592150 1_3:+12891765 Msa0104740:CDS
ATCATTGGCCGTGTAAACTC+CGG 0.595715 1_3:-12891773 None:intergenic
GATGTTGTTACTTGTTTGCG+TGG 0.596003 1_3:+12892191 Msa0104740:three_prime_UTR
TTAAGACACTTCCATTAACA+TGG 0.596362 1_3:+12889891 Msa0104740:CDS
GATTAGAAGGTTGCAAGAGA+GGG 0.596681 1_3:+12891906 Msa0104740:CDS
ACAGAAAACAACCCATACTT+GGG 0.596760 1_3:+12891448 Msa0104740:CDS
CTTCTAGAAGTGGACCCCAC+AGG 0.596989 1_3:+12891634 Msa0104740:CDS
ATCAATGGTCTTGATGGTGT+TGG 0.597547 1_3:+12890277 Msa0104740:CDS
CAACTAAACTTGACCCCATG+TGG 0.598754 1_3:-12891955 None:intergenic
TGAATTTACTGATCAGGTGA+AGG 0.600944 1_3:+12890156 Msa0104740:CDS
GTTGGTTGGTGATATGGTGA+CGG 0.602166 1_3:+12890225 Msa0104740:CDS
GCTTGAAGGATTAACGGGTG+AGG 0.603070 1_3:+12891843 Msa0104740:CDS
TGGTTCCAGTCCAAGCCCGT+GGG 0.608947 1_3:+12890309 Msa0104740:CDS
CGAGCAACATTTGTATCACA+CGG 0.621285 1_3:+12891496 Msa0104740:CDS
ATTTGTATCACACGGAAACA+CGG 0.623224 1_3:+12891504 Msa0104740:CDS
TTGATCATACTCTGATACGT+CGG 0.624523 1_3:-12890259 None:intergenic
CAAATGGCAATGAAGGATCA+GGG 0.626451 1_3:-12890132 None:intergenic
CAACAAAATACTCATCAGGA+AGG 0.632422 1_3:-12890201 None:intergenic
GGTGGTAATAGGTGGCGGTG+TGG 0.634289 1_3:-12889941 None:intergenic
TGGCTAGCATTGCTCCTGTG+GGG 0.636911 1_3:-12891648 None:intergenic
CTCGTAAGATGAAACCACAT+GGG 0.643536 1_3:+12891941 Msa0104740:CDS
CTGCTGAAGAAAATAGACAT+GGG 0.645814 1_3:+12890353 Msa0104740:CDS
GCTCGTAAGATGAAACCACA+TGG 0.658763 1_3:+12891940 Msa0104740:CDS
TGCTGAAGAAAATAGACATG+GGG 0.668352 1_3:+12890354 Msa0104740:CDS
ACTGCTGAAGAAAATAGACA+TGG 0.671679 1_3:+12890352 Msa0104740:CDS
TTTACCACTACTAAAGCCAG+TGG 0.671826 1_3:+12890081 Msa0104740:CDS
ACTAATCTTGACACTAAACA+AGG 0.675822 1_3:+12892074 Msa0104740:three_prime_UTR
GCTCGCTTAGCGAAAGAAGG+CGG 0.694123 1_3:+12891526 Msa0104740:CDS
GGTCCTTCGTGCGATCACGA+AGG 0.703598 1_3:-12890180 None:intergenic
GGATCAGGGAGAAAATTCTG+TGG 0.710660 1_3:-12890118 None:intergenic
GGTCCTTCGTGATCGCACGA+AGG 0.710721 1_3:+12890177 Msa0104740:CDS
TCGTAAGATGAAACCACATG+GGG 0.759922 1_3:+12891942 Msa0104740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAATAATTAGATAAAT+TGG + chr1_3:12890550-12890569 Msa0104740:intron 10.0%
!! ATTAAAAATAAATCTATGAT+AGG + chr1_3:12890904-12890923 Msa0104740:intron 10.0%
!! TTAAAAATAAATCTATGATA+GGG + chr1_3:12890905-12890924 Msa0104740:intron 10.0%
!! AAAAAAAAAAAAAAAAAACG+AGG - chr1_3:12890963-12890982 None:intergenic 10.0%
!! CATATTATTAATTAAAGTGA+TGG + chr1_3:12890587-12890606 Msa0104740:intron 15.0%
!!! TTTTTTACTTTCTAACTAAA+TGG + chr1_3:12891362-12891381 Msa0104740:intron 15.0%
!! ATATGATCTATACTAGTTAA+TGG - chr1_3:12890828-12890847 None:intergenic 20.0%
!! TTGAAAAAATATAGAATCCT+CGG + chr1_3:12890982-12891001 Msa0104740:intron 20.0%
!!! AATGCTCTGTTTTTTTTTTT+TGG + chr1_3:12891036-12891055 Msa0104740:intron 20.0%
!!! AATTGATTTTTCATGTGTTA+CGG + chr1_3:12891324-12891343 Msa0104740:intron 20.0%
!!! TAGTTGGATTTTTAATAATG+AGG + chr1_3:12891972-12891991 Msa0104740:CDS 20.0%
!! AGTTTGAATATGCATTGTTT+TGG - chr1_3:12889861-12889880 None:intergenic 25.0%
!!! TTGAAAATTTCTAGCTTTTC+TGG - chr1_3:12890031-12890050 None:intergenic 25.0%
!! TTTTCAAATCACTAGAAAAC+TGG + chr1_3:12890044-12890063 Msa0104740:CDS 25.0%
! TTTCAAATCACTAGAAAACT+GGG + chr1_3:12890045-12890064 Msa0104740:CDS 25.0%
! TGATCAGTAAATTCATCAAA+TGG - chr1_3:12890151-12890170 None:intergenic 25.0%
! ATTTGATGAATTTACTGATC+AGG + chr1_3:12890150-12890169 Msa0104740:CDS 25.0%
!! TTATTTGTATCTTTCTGGAA+GGG + chr1_3:12890390-12890409 Msa0104740:CDS 25.0%
! AAAACTGTTCAGTATCTTAT+TGG + chr1_3:12890433-12890452 Msa0104740:CDS 25.0%
!! TGTACATTTTATTGCAATCT+AGG + chr1_3:12890521-12890540 Msa0104740:intron 25.0%
! CAAAACAGAACATTATGTTT+CGG - chr1_3:12891063-12891082 None:intergenic 25.0%
! TCATGTAACATAAAAGTGAT+GGG - chr1_3:12891132-12891151 None:intergenic 25.0%
! TTCATGTAACATAAAAGTGA+TGG - chr1_3:12891133-12891152 None:intergenic 25.0%
!! ACTTTTATGTTACATGAATG+AGG + chr1_3:12891135-12891154 Msa0104740:intron 25.0%
! ACTACTTAGAAGAAAAGTTT+CGG - chr1_3:12891244-12891263 None:intergenic 25.0%
!!! TAATTGATTTTTCACATGTG+TGG + chr1_3:12891287-12891306 Msa0104740:intron 25.0%
!!! AATTGATTTTTCACATGTGT+GGG + chr1_3:12891288-12891307 Msa0104740:intron 25.0%
!! GTAGACATTATTTTCAACTT+AGG - chr1_3:12891394-12891413 None:intergenic 25.0%
!!! TTTTGGAGTTTTTAATCGAA+CGG + chr1_3:12891806-12891825 Msa0104740:CDS 25.0%
!!! ATGTATTTTGACTCTAATGT+TGG + chr1_3:12892146-12892165 Msa0104740:three_prime_UTR 25.0%
! TTTTGGATTTGATACTAGCT+AGG - chr1_3:12889844-12889863 None:intergenic 30.0%
TTAAGACACTTCCATTAACA+TGG + chr1_3:12889891-12889910 Msa0104740:CDS 30.0%
! TTTGGAATGTTCCATGTTAA+TGG - chr1_3:12889905-12889924 None:intergenic 30.0%
!!! AAAATTTCTAGCTTTTCTGG+TGG - chr1_3:12890028-12890047 None:intergenic 30.0%
AATTCATCAAATGGCAATGA+AGG - chr1_3:12890142-12890161 None:intergenic 30.0%
AACACAACAAAATACTCATC+AGG - chr1_3:12890208-12890227 None:intergenic 30.0%
!! TGATGAGTATTTTGTTGTGT+TGG + chr1_3:12890207-12890226 Msa0104740:CDS 30.0%
AGGACTTATTTGTATCTTTC+TGG + chr1_3:12890385-12890404 Msa0104740:CDS 30.0%
CTTATTTGTATCTTTCTGGA+AGG + chr1_3:12890389-12890408 Msa0104740:CDS 30.0%
!! TTAAAGTGATGGATAGTAGT+AGG + chr1_3:12890598-12890617 Msa0104740:intron 30.0%
! GCAACATTGTAATTTGTAAG+AGG - chr1_3:12890802-12890821 None:intergenic 30.0%
ATGTAACATAAAAGTGATGG+GGG - chr1_3:12891130-12891149 None:intergenic 30.0%
CATGTAACATAAAAGTGATG+GGG - chr1_3:12891131-12891150 None:intergenic 30.0%
!! ACATTGCATTTTGTTTGCAT+TGG + chr1_3:12891412-12891431 Msa0104740:intron 30.0%
TGTATACAAATCCCAAGTAT+GGG - chr1_3:12891462-12891481 None:intergenic 30.0%
CAAAATATCAGCGTAATCAT+TGG - chr1_3:12891791-12891810 None:intergenic 30.0%
! CAATGATTACGCTGATATTT+TGG + chr1_3:12891789-12891808 Msa0104740:CDS 30.0%
ACTAATCTTGACACTAAACA+AGG + chr1_3:12892074-12892093 Msa0104740:three_prime_UTR 30.0%
! TGAAAAATAGTTGGGACTTT+TGG + chr1_3:12892215-12892234 Msa0104740:three_prime_UTR 30.0%
!!! GTTTTGGAGATTTTTAGCGA+CGG - chr1_3:12889995-12890014 None:intergenic 35.0%
TGAATTTACTGATCAGGTGA+AGG + chr1_3:12890156-12890175 Msa0104740:CDS 35.0%
CAACAAAATACTCATCAGGA+AGG - chr1_3:12890204-12890223 None:intergenic 35.0%
!! GAGTATTTTGTTGTGTTGGT+TGG + chr1_3:12890211-12890230 Msa0104740:CDS 35.0%
TTGATCATACTCTGATACGT+CGG - chr1_3:12890262-12890281 None:intergenic 35.0%
ACGTATCAGAGTATGATCAA+TGG + chr1_3:12890262-12890281 Msa0104740:CDS 35.0%
! AGTATGATCAATGGTCTTGA+TGG + chr1_3:12890271-12890290 Msa0104740:CDS 35.0%
ACTGCTGAAGAAAATAGACA+TGG + chr1_3:12890352-12890371 Msa0104740:CDS 35.0%
CTGCTGAAGAAAATAGACAT+GGG + chr1_3:12890353-12890372 Msa0104740:CDS 35.0%
TGCTGAAGAAAATAGACATG+GGG + chr1_3:12890354-12890373 Msa0104740:CDS 35.0%
ACAGAAAACAACCCATACTT+GGG + chr1_3:12891448-12891467 Msa0104740:CDS 35.0%
GTGTATACAAATCCCAAGTA+TGG - chr1_3:12891463-12891482 None:intergenic 35.0%
ATTTGTATCACACGGAAACA+CGG + chr1_3:12891504-12891523 Msa0104740:CDS 35.0%
TTTCTCCATCATATCACCAA+TGG - chr1_3:12891671-12891690 None:intergenic 35.0%
GGAGAAAAAAATCACAATGC+CGG + chr1_3:12891684-12891703 Msa0104740:CDS 35.0%
GGAAAAGCTTGAAGGATTAA+CGG + chr1_3:12891837-12891856 Msa0104740:CDS 35.0%
GAAAAGCTTGAAGGATTAAC+GGG + chr1_3:12891838-12891857 Msa0104740:CDS 35.0%
AGACTAGAGATTAAGAGCTA+AGG + chr1_3:12892034-12892053 Msa0104740:three_prime_UTR 35.0%
GACTAGAGATTAAGAGCTAA+GGG + chr1_3:12892035-12892054 Msa0104740:three_prime_UTR 35.0%
TGACACTAAACAAGGAACTT+TGG + chr1_3:12892082-12892101 Msa0104740:three_prime_UTR 35.0%
GACACTAAACAAGGAACTTT+GGG + chr1_3:12892083-12892102 Msa0104740:three_prime_UTR 35.0%
!! TTTTGACTCTAATGTTGGAG+AGG + chr1_3:12892151-12892170 Msa0104740:three_prime_UTR 35.0%
TTGCGTGGATGAAAAATAGT+TGG + chr1_3:12892206-12892225 Msa0104740:three_prime_UTR 35.0%
TGCGTGGATGAAAAATAGTT+GGG + chr1_3:12892207-12892226 Msa0104740:three_prime_UTR 35.0%
!!! ATTTTGGTGGTGGTAATAGG+TGG - chr1_3:12889952-12889971 None:intergenic 40.0%
!!! TGGATTTTGGTGGTGGTAAT+AGG - chr1_3:12889955-12889974 None:intergenic 40.0%
TTTACCACTACTAAAGCCAG+TGG + chr1_3:12890081-12890100 Msa0104740:CDS 40.0%
CAAATGGCAATGAAGGATCA+GGG - chr1_3:12890135-12890154 None:intergenic 40.0%
TCAAATGGCAATGAAGGATC+AGG - chr1_3:12890136-12890155 None:intergenic 40.0%
!! TGTTGTGTTGGTTGGTGATA+TGG + chr1_3:12890219-12890238 Msa0104740:CDS 40.0%
!! ATCAATGGTCTTGATGGTGT+TGG + chr1_3:12890277-12890296 Msa0104740:CDS 40.0%
! ATAGACATGGGGATTTGCTT+AGG + chr1_3:12890365-12890384 Msa0104740:CDS 40.0%
!! TCTTTCTGGAAGGGTTGATA+TGG + chr1_3:12890399-12890418 Msa0104740:CDS 40.0%
GTTCAGTATCTTATTGGAGC+TGG + chr1_3:12890439-12890458 Msa0104740:CDS 40.0%
!! GGATAGTAGTAGGAGTGTTT+AGG + chr1_3:12890608-12890627 Msa0104740:intron 40.0%
!! TAGTAGTAGGAGTGTTTAGG+TGG + chr1_3:12890611-12890630 Msa0104740:intron 40.0%
!! GCATTTTGTTTGCATTGGAC+AGG + chr1_3:12891417-12891436 Msa0104740:intron 40.0%
GACAGAAAACAACCCATACT+TGG + chr1_3:12891447-12891466 Msa0104740:CDS 40.0%
ATACAAATGTTGCTCGCTCT+TGG - chr1_3:12891492-12891511 None:intergenic 40.0%
CGAGCAACATTTGTATCACA+CGG + chr1_3:12891496-12891515 Msa0104740:CDS 40.0%
! GTTCTCATGACGCTTTTCAT+CGG - chr1_3:12891590-12891609 None:intergenic 40.0%
! GGTCTTGTCCATCATACATA+AGG - chr1_3:12891714-12891733 None:intergenic 40.0%
! TGGAGTTTTTAATCGAACGG+TGG + chr1_3:12891809-12891828 Msa0104740:CDS 40.0%
TTTAATCGAACGGTGGAGAT+TGG + chr1_3:12891816-12891835 Msa0104740:CDS 40.0%
TGGAGATTGGAAAAGCTTGA+AGG + chr1_3:12891829-12891848 Msa0104740:CDS 40.0%
! ATTAACGGGTGAGGGAAAAA+GGG + chr1_3:12891852-12891871 Msa0104740:CDS 40.0%
TCTTGCAACCTTCTAATCCT+AGG - chr1_3:12891904-12891923 None:intergenic 40.0%
GATTAGAAGGTTGCAAGAGA+GGG + chr1_3:12891906-12891925 Msa0104740:CDS 40.0%
CTCGTAAGATGAAACCACAT+GGG + chr1_3:12891941-12891960 Msa0104740:CDS 40.0%
TCGTAAGATGAAACCACATG+GGG + chr1_3:12891942-12891961 Msa0104740:CDS 40.0%
AAACAAGGAACTTTGGGTGA+TGG + chr1_3:12892089-12892108 Msa0104740:three_prime_UTR 40.0%
AACAAGGAACTTTGGGTGAT+GGG + chr1_3:12892090-12892109 Msa0104740:three_prime_UTR 40.0%
CTCTAATGTTGGAGAGGATA+TGG + chr1_3:12892157-12892176 Msa0104740:three_prime_UTR 40.0%
! GATGTTGTTACTTGTTTGCG+TGG + chr1_3:12892191-12892210 Msa0104740:three_prime_UTR 40.0%
!!! TTGGAGATTTTTAGCGACGG+TGG - chr1_3:12889992-12890011 None:intergenic 45.0%
!! CTTTTCTGGTGGCATTGAGT+GGG - chr1_3:12890017-12890036 None:intergenic 45.0%
!! TGTTCCACTGGCTTTAGTAG+TGG - chr1_3:12890088-12890107 None:intergenic 45.0%
TAAAGCCAGTGGAACAATGC+TGG + chr1_3:12890092-12890111 Msa0104740:CDS 45.0%
GGATCAGGGAGAAAATTCTG+TGG - chr1_3:12890121-12890140 None:intergenic 45.0%
!! GTTGGTTGGTGATATGGTGA+CGG + chr1_3:12890225-12890244 Msa0104740:CDS 45.0%
!! TCTTGATGGTGTTGGTGATG+AGG + chr1_3:12890285-12890304 Msa0104740:CDS 45.0%
GTATCTTATTGGAGCTGGCA+TGG + chr1_3:12890444-12890463 Msa0104740:CDS 45.0%
CAACATATGCGATCAAACCG+AGG - chr1_3:12891002-12891021 None:intergenic 45.0%
!! TATGGGTTGTTTTCTGTCCC+GGG - chr1_3:12891445-12891464 None:intergenic 45.0%
!! GTATGGGTTGTTTTCTGTCC+CGG - chr1_3:12891446-12891465 None:intergenic 45.0%
! GCTAGCCATTGGTGATATGA+TGG + chr1_3:12891663-12891682 Msa0104740:CDS 45.0%
CCATCATACATAAGGTGTGC+CGG - chr1_3:12891706-12891725 None:intergenic 45.0%
! CGAAGTGCTCGAATAGCTTA+GGG - chr1_3:12891735-12891754 None:intergenic 45.0%
TAAGCTATTCGAGCACTTCG+CGG + chr1_3:12891735-12891754 Msa0104740:CDS 45.0%
ATCATTGGCCGTGTAAACTC+CGG - chr1_3:12891776-12891795 None:intergenic 45.0%
! CTTGAAGGATTAACGGGTGA+GGG + chr1_3:12891844-12891863 Msa0104740:CDS 45.0%
! GATTAACGGGTGAGGGAAAA+AGG + chr1_3:12891851-12891870 Msa0104740:CDS 45.0%
GGTTAGCACCTAGGATTAGA+AGG + chr1_3:12891893-12891912 Msa0104740:CDS 45.0%
GGATTAGAAGGTTGCAAGAG+AGG + chr1_3:12891905-12891924 Msa0104740:CDS 45.0%
GCTCGTAAGATGAAACCACA+TGG + chr1_3:12891940-12891959 Msa0104740:CDS 45.0%
CAACTAAACTTGACCCCATG+TGG - chr1_3:12891958-12891977 None:intergenic 45.0%
CACATGGGGTCAAGTTTAGT+TGG + chr1_3:12891956-12891975 Msa0104740:CDS 45.0%
!!! TTTAATTTCATTTAATATTT+TGG + chr1_3:12890487-12890506 Msa0104740:intron 5.0%
!!! AAAAGATTTATTTTTTTTTA+GGG - chr1_3:12890875-12890894 None:intergenic 5.0%
!!! AAAAAGATTTATTTTTTTTT+AGG - chr1_3:12890876-12890895 None:intergenic 5.0%
!!! GGTCGGTGGTTGATGGTTTT+TGG - chr1_3:12889923-12889942 None:intergenic 50.0%
!! TTGGTGGTGGTAATAGGTGG+CGG - chr1_3:12889949-12889968 None:intergenic 50.0%
!!! GTGATGGTGGATTTTGGTGG+TGG - chr1_3:12889962-12889981 None:intergenic 50.0%
!!! GTGGTGATGGTGGATTTTGG+TGG - chr1_3:12889965-12889984 None:intergenic 50.0%
!!! GTGGTGGTGATGGTGGATTT+TGG - chr1_3:12889968-12889987 None:intergenic 50.0%
!!! GATTTTTAGCGACGGTGGTG+TGG - chr1_3:12889987-12890006 None:intergenic 50.0%
!! TGGTGGCATTGAGTGGGTTT+TGG - chr1_3:12890011-12890030 None:intergenic 50.0%
!! GCTTTTCTGGTGGCATTGAG+TGG - chr1_3:12890018-12890037 None:intergenic 50.0%
!! GATGGTGTTGGTGATGAGGT+TGG + chr1_3:12890289-12890308 Msa0104740:CDS 50.0%
AGTCCAAGCTCGTGTCCAAA+CGG - chr1_3:12890335-12890354 None:intergenic 50.0%
!! ATGGGTTGTTTTCTGTCCCG+GGG - chr1_3:12891444-12891463 None:intergenic 50.0%
! CGTCGAGAAGCTTCTAGAAG+TGG + chr1_3:12891624-12891643 Msa0104740:CDS 50.0%
ATGGCTAGCATTGCTCCTGT+GGG - chr1_3:12891652-12891671 None:intergenic 50.0%
AATGGCTAGCATTGCTCCTG+TGG - chr1_3:12891653-12891672 None:intergenic 50.0%
ACAGGAGCAATGCTAGCCAT+TGG + chr1_3:12891652-12891671 Msa0104740:CDS 50.0%
CCGGCACACCTTATGTATGA+TGG + chr1_3:12891703-12891722 Msa0104740:CDS 50.0%
! GCGAAGTGCTCGAATAGCTT+AGG - chr1_3:12891736-12891755 None:intergenic 50.0%
! GCTTGAAGGATTAACGGGTG+AGG + chr1_3:12891843-12891862 Msa0104740:CDS 50.0%
!! TTGTGTGTGGGTTAGCACCT+AGG + chr1_3:12891884-12891903 Msa0104740:CDS 50.0%
! GTAATAGGTGGCGGTGTGGT+CGG - chr1_3:12889940-12889959 None:intergenic 55.0%
! TTTTAGCGACGGTGGTGTGG+TGG - chr1_3:12889984-12890003 None:intergenic 55.0%
GTTTGCATTGGACAGGACCC+CGG + chr1_3:12891424-12891443 Msa0104740:intron 55.0%
TTTGCATTGGACAGGACCCC+GGG + chr1_3:12891425-12891444 Msa0104740:intron 55.0%
ACGGCTCGCTTAGCGAAAGA+AGG + chr1_3:12891523-12891542 Msa0104740:CDS 55.0%
GCTCGCTTAGCGAAAGAAGG+CGG + chr1_3:12891526-12891545 Msa0104740:CDS 55.0%
!! ACGCTTTTCATCGGCTGCGA+TGG - chr1_3:12891581-12891600 None:intergenic 55.0%
! CTTCTAGAAGTGGACCCCAC+AGG + chr1_3:12891634-12891653 Msa0104740:CDS 55.0%
TGGCTAGCATTGCTCCTGTG+GGG - chr1_3:12891651-12891670 None:intergenic 55.0%
ACAGCGCACCGGAGTTTACA+CGG + chr1_3:12891765-12891784 Msa0104740:CDS 55.0%
! AGGGCGCAGGATTTTGTGTG+TGG + chr1_3:12891871-12891890 Msa0104740:CDS 55.0%
! GGGCGCAGGATTTTGTGTGT+GGG + chr1_3:12891872-12891891 Msa0104740:CDS 55.0%
!! GGAACTTTGGGTGATGGGAG+TGG + chr1_3:12892095-12892114 Msa0104740:three_prime_UTR 55.0%
! GGTGGTAATAGGTGGCGGTG+TGG - chr1_3:12889944-12889963 None:intergenic 60.0%
GGCTGCCAGCATTGTTCCAC+TGG - chr1_3:12890100-12890119 None:intergenic 60.0%
GGTCCTTCGTGATCGCACGA+AGG + chr1_3:12890177-12890196 Msa0104740:CDS 60.0%
GGTCCTTCGTGCGATCACGA+AGG - chr1_3:12890183-12890202 None:intergenic 60.0%
TTGGTTCCAGTCCAAGCCCG+TGG + chr1_3:12890308-12890327 Msa0104740:CDS 60.0%
TGGTTCCAGTCCAAGCCCGT+GGG + chr1_3:12890309-12890328 Msa0104740:CDS 60.0%
CCACATATGCGCGCAAGCAC+TGG - chr1_3:12891556-12891575 None:intergenic 60.0%
CCAGTGCTTGCGCGCATATG+TGG + chr1_3:12891553-12891572 Msa0104740:CDS 60.0%
GCGGTGTGGTCGGTGGTTGA+TGG - chr1_3:12889930-12889949 None:intergenic 65.0%
! ATAGGTGGCGGTGTGGTCGG+TGG - chr1_3:12889937-12889956 None:intergenic 65.0%
!! CGGTGGTGTGGTGGTGATGG+TGG - chr1_3:12889975-12889994 None:intergenic 65.0%
! CGACGGTGGTGTGGTGGTGA+TGG - chr1_3:12889978-12889997 None:intergenic 65.0%
CTCGTGTCCAAACGGCCCAC+GGG - chr1_3:12890327-12890346 None:intergenic 65.0%
GCTCGTGTCCAAACGGCCCA+CGG - chr1_3:12890328-12890347 None:intergenic 65.0%
GGGCCGTTTGGACACGAGCT+TGG + chr1_3:12890329-12890348 Msa0104740:CDS 65.0%
ATTCGAGCACTTCGCGGCCG+TGG + chr1_3:12891741-12891760 Msa0104740:CDS 65.0%
AACTCCGGTGCGCTGTGCCA+CGG - chr1_3:12891761-12891780 None:intergenic 65.0%
! GGGTGAGGGAAAAAGGGCGC+AGG + chr1_3:12891858-12891877 Msa0104740:CDS 65.0%
AACGGCCCACGGGCTTGGAC+TGG - chr1_3:12890317-12890336 None:intergenic 70.0%
GTCCAAGCCCGTGGGCCGTT+TGG + chr1_3:12890317-12890336 Msa0104740:CDS 70.0%
GTCCAAACGGCCCACGGGCT+TGG - chr1_3:12890322-12890341 None:intergenic 70.0%
! GCGGCCGTGGCACAGCGCAC+CGG + chr1_3:12891754-12891773 Msa0104740:CDS 80.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 12889826 12892335 12889826 ID=Msa0104740;Name=Msa0104740
chr1_3 mRNA 12889826 12892335 12889826 ID=Msa0104740-mRNA-1;Parent=Msa0104740;Name=Msa0104740-mRNA-1;_AED=0.03;_eAED=0.03;_QI=40|1|1|1|1|1|2|330|388
chr1_3 exon 12889826 12890465 12889826 ID=Msa0104740-mRNA-1:exon:5058;Parent=Msa0104740-mRNA-1
chr1_3 exon 12891439 12892335 12891439 ID=Msa0104740-mRNA-1:exon:5059;Parent=Msa0104740-mRNA-1
chr1_3 five_prime_UTR 12889826 12889865 12889826 ID=Msa0104740-mRNA-1:five_prime_utr;Parent=Msa0104740-mRNA-1
chr1_3 CDS 12889866 12890465 12889866 ID=Msa0104740-mRNA-1:cds;Parent=Msa0104740-mRNA-1
chr1_3 CDS 12891439 12892005 12891439 ID=Msa0104740-mRNA-1:cds;Parent=Msa0104740-mRNA-1
chr1_3 three_prime_UTR 12892006 12892335 12892006 ID=Msa0104740-mRNA-1:three_prime_utr;Parent=Msa0104740-mRNA-1
Gene Sequence

>Msa0104740

ATGCATATTCAAACTTGTCACTCAATTAAGACACTTCCATTAACATGGAACATTCCAAAAACCATCAACCACCGACCACACCGCCACCTATTACCACCACCAAAATCCACCATCACCACCACACCACCGTCGCTAAAAATCTCCAAAACCCACTCAATGCCACCAGAAAAGCTAGAAATTTTCAAATCACTAGAAAACTGGGCTTCTGAGTCTGTTTTACCACTACTAAAGCCAGTGGAACAATGCTGGCAGCCACAGAATTTTCTCCCTGATCCTTCATTGCCATTTGATGAATTTACTGATCAGGTGAAGGTCCTTCGTGATCGCACGAAGGACCTTCCTGATGAGTATTTTGTTGTGTTGGTTGGTGATATGGTGACGGAAGACGCACTTCCGACGTATCAGAGTATGATCAATGGTCTTGATGGTGTTGGTGATGAGGTTGGTTCCAGTCCAAGCCCGTGGGCCGTTTGGACACGAGCTTGGACTGCTGAAGAAAATAGACATGGGGATTTGCTTAGGACTTATTTGTATCTTTCTGGAAGGGTTGATATGGAGATGATTGAGAAAACTGTTCAGTATCTTATTGGAGCTGGCATGGACCCCGGGACAGAAAACAACCCATACTTGGGATTTGTATACACATCATTCCAAGAGCGAGCAACATTTGTATCACACGGAAACACGGCTCGCTTAGCGAAAGAAGGCGGTGATCCAGTGCTTGCGCGCATATGTGGCACCATCGCAGCCGATGAAAAGCGTCATGAGAACGCATACTCAAAAATCGTCGAGAAGCTTCTAGAAGTGGACCCCACAGGAGCAATGCTAGCCATTGGTGATATGATGGAGAAAAAAATCACAATGCCGGCACACCTTATGTATGATGGACAAGACCCTAAGCTATTCGAGCACTTCGCGGCCGTGGCACAGCGCACCGGAGTTTACACGGCCAATGATTACGCTGATATTTTGGAGTTTTTAATCGAACGGTGGAGATTGGAAAAGCTTGAAGGATTAACGGGTGAGGGAAAAAGGGCGCAGGATTTTGTGTGTGGGTTAGCACCTAGGATTAGAAGGTTGCAAGAGAGGGCTGATGCACGTGCTCGTAAGATGAAACCACATGGGGTCAAGTTTAGTTGGATTTTTAATAATGAGGTTGTTTTGTGA
Protein sequence

>Msa0104740

MHIQTCHSIKTLPLTWNIPKTINHRPHRHLLPPPKSTITTTPPSLKISKTHSMPPEKLEIFKSLENWASESVLPLLKPVEQCWQPQNFLPDPSLPFDEFTDQVKVLRDRTKDLPDEYFVVLVGDMVTEDALPTYQSMINGLDGVGDEVGSSPSPWAVWTRAWTAEENRHGDLLRTYLYLSGRVDMEMIEKTVQYLIGAGMDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKEGGDPVLARICGTIAADEKRHENAYSKIVEKLLEVDPTGAMLAIGDMMEKKITMPAHLMYDGQDPKLFEHFAAVAQRTGVYTANDYADILEFLIERWRLEKLEGLTGEGKRAQDFVCGLAPRIRRLQERADARARKMKPHGVKFSWIFNNEVVL*