AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0228690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0228690 MtrunA17_Chr1g0159321 97.179 390 9 1 1 388 1 390 0.0 790
Msa0228690 MtrunA17_Chr3g0126011 75.291 344 82 1 45 388 26 366 0.0 551
Msa0228690 MtrunA17_Chr3g0125981 71.745 361 92 2 26 386 18 368 0.0 539
Msa0228690 MtrunA17_Chr3g0125991 71.788 358 96 2 33 388 12 366 0.0 538
Msa0228690 MtrunA17_Chr3g0125781 71.429 350 97 1 39 388 23 369 0.0 534
Msa0228690 MtrunA17_Chr5g0406961 70.487 349 99 2 40 388 100 444 0.0 530
Msa0228690 MtrunA17_Chr4g0045661 70.487 349 99 2 40 388 49 393 0.0 528
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0228690 AT1G43800.1 77.654 358 76 3 33 386 32 389 0.0 587
Msa0228690 AT3G02630.1 69.855 345 101 2 42 386 53 394 0.0 520
Msa0228690 AT2G43710.2 69.054 349 104 2 40 388 57 401 0.0 515
Msa0228690 AT2G43710.1 69.054 349 104 2 40 388 57 401 0.0 515
Msa0228690 AT5G16240.1 67.246 345 110 2 42 386 51 392 6.36e-178 501
Msa0228690 AT5G16230.1 67.353 340 109 2 50 388 63 401 5.36e-174 491
Msa0228690 AT3G02610.2 68.222 343 103 4 50 388 73 413 5.07e-173 489
Msa0228690 AT3G02610.1 68.222 343 103 4 50 388 71 411 5.95e-173 489
Msa0228690 AT3G02620.1 63.904 374 119 7 26 388 35 403 1.72e-170 482
Msa0228690 AT3G02620.2 63.904 374 119 7 26 388 38 406 1.97e-170 482
Msa0228690 AT5G16230.2 66.871 326 106 2 64 388 1 325 1.60e-165 466
Msa0228690 AT3G02620.3 66.869 329 103 4 64 388 1 327 1.99e-162 459

Find 117 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGACTTATTTGTATCTTTC+TGG 0.182339 2_2:+14027 Msa0228690:CDS
CAATGATTACGCTGATATTT+TGG 0.196546 2_2:+15431 Msa0228690:CDS
AAAACTGTTCAGTATCTTAT+TGG 0.203529 2_2:+14075 Msa0228690:CDS
GTGTATACAAATCCCAAGTA+TGG 0.240289 2_2:-15102 None:intergenic
GATTAACGGGTGAGGGAAAA+AGG 0.240677 2_2:+15493 Msa0228690:CDS
TGTTGTGTTGGTTGGTGATA+TGG 0.248245 2_2:+13861 Msa0228690:CDS
GTGGTGGTGATGGTGGATTT+TGG 0.262583 2_2:-13607 None:intergenic
TTTGGAATGTTCCATGTTAA+TGG 0.266021 2_2:-13544 None:intergenic
TGGTGGCATTGAGTGGGTTT+TGG 0.304471 2_2:-13650 None:intergenic
AGTTTGAATATGCATTGTTT+TGG 0.317713 2_2:-13500 None:intergenic
CTCTAATGTTGGAGAGGATA+TGG 0.327487 2_2:+15799 Msa0228690:three_prime_UTR
GTCCAAGCCCGTGGGCCGTT+TGG 0.332806 2_2:+13959 Msa0228690:CDS
TCTTTCTGGAAGGGTTGATA+TGG 0.335225 2_2:+14041 Msa0228690:CDS
GACACTAAACAAGGAACTTT+GGG 0.344459 2_2:+15725 Msa0228690:three_prime_UTR
GTCCAAACGGCCCACGGGCT+TGG 0.353727 2_2:-13961 None:intergenic
GCGGTGTGGTCGGTGGTTGA+TGG 0.380740 2_2:-13569 None:intergenic
GGCTGCCAGCATTGTTCCAC+TGG 0.386228 2_2:-13739 None:intergenic
TGACACTAAACAAGGAACTT+TGG 0.388024 2_2:+15724 Msa0228690:three_prime_UTR
AACGGCCCACGGGCTTGGAC+TGG 0.398332 2_2:-13956 None:intergenic
CACATGGGGTCAAGTTTAGT+TGG 0.400607 2_2:+15598 Msa0228690:CDS
TTTAATCGAACGGTGGAGAT+TGG 0.401193 2_2:+15458 Msa0228690:CDS
ATACAAATGTTGCTCGCTCT+TGG 0.405320 2_2:-15131 None:intergenic
GACAGAAAACAACCCATACT+TGG 0.410965 2_2:+15089 Msa0228690:CDS
GGTTAGCACCTAGGATTAGA+AGG 0.411387 2_2:+15535 Msa0228690:CDS
ATTTGATGAATTTACTGATC+AGG 0.419139 2_2:+13792 Msa0228690:CDS
TCAAATGGCAATGAAGGATC+AGG 0.420107 2_2:-13775 None:intergenic
CCACATATGCGCGCAAGCAC+TGG 0.427060 2_2:-15195 None:intergenic
GCGAAGTGCTCGAATAGCTT+AGG 0.427800 2_2:-15375 None:intergenic
CAAAATATCAGCGTAATCAT+TGG 0.433099 2_2:-15430 None:intergenic
GTAATAGGTGGCGGTGTGGT+CGG 0.450987 2_2:-13579 None:intergenic
GAAAAGCTTGAAGGATTAAC+GGG 0.453295 2_2:+15480 Msa0228690:CDS
TTTGCATTGGACAGGACCCC+GGG 0.454311 2_2:+15067 Msa0228690:intron
CGACGGTGGTGTGGTGGTGA+TGG 0.457737 2_2:-13617 None:intergenic
TGATCAGTAAATTCATCAAA+TGG 0.459663 2_2:-13790 None:intergenic
ATAGACATGGGGATTTGCTT+AGG 0.460553 2_2:+14007 Msa0228690:CDS
GGAAAAGCTTGAAGGATTAA+CGG 0.464892 2_2:+15479 Msa0228690:CDS
GGGCCGTTTGGACACGAGCT+TGG 0.467549 2_2:+13971 Msa0228690:CDS
ATTCGAGCACTTCGCGGCCG+TGG 0.474737 2_2:+15383 Msa0228690:CDS
GTTCAGTATCTTATTGGAGC+TGG 0.477043 2_2:+14081 Msa0228690:CDS
AATTCATCAAATGGCAATGA+AGG 0.478762 2_2:-13781 None:intergenic
CGAAGTGCTCGAATAGCTTA+GGG 0.481594 2_2:-15374 None:intergenic
TTGTGTGTGGGTTAGCACCT+AGG 0.482074 2_2:+15526 Msa0228690:CDS
AACAAGGAACTTTGGGTGAT+GGG 0.486012 2_2:+15732 Msa0228690:three_prime_UTR
TCTTGCAACCTTCTAATCCT+AGG 0.486803 2_2:-15543 None:intergenic
AATGGCTAGCATTGCTCCTG+TGG 0.488560 2_2:-15292 None:intergenic
AACACAACAAAATACTCATC+AGG 0.489365 2_2:-13847 None:intergenic
CCGGCACACCTTATGTATGA+TGG 0.493314 2_2:+15345 Msa0228690:CDS
GGTCTTGTCCATCATACATA+AGG 0.494290 2_2:-15353 None:intergenic
AGACTAGAGATTAAGAGCTA+AGG 0.494866 2_2:+15676 Msa0228690:three_prime_UTR
TTATTTGTATCTTTCTGGAA+GGG 0.495330 2_2:+14032 Msa0228690:CDS
GACTAGAGATTAAGAGCTAA+GGG 0.495595 2_2:+15677 Msa0228690:three_prime_UTR
CGGTGGTGTGGTGGTGATGG+TGG 0.503987 2_2:-13614 None:intergenic
GATGGTGTTGGTGATGAGGT+TGG 0.504177 2_2:+13931 Msa0228690:CDS
GCGGCCGTGGCACAGCGCAC+CGG 0.504431 2_2:+15396 Msa0228690:CDS
TGGAGATTGGAAAAGCTTGA+AGG 0.504527 2_2:+15471 Msa0228690:CDS
CCAGTGCTTGCGCGCATATG+TGG 0.504826 2_2:+15195 Msa0228690:CDS
ATGGCTAGCATTGCTCCTGT+GGG 0.506752 2_2:-15291 None:intergenic
GGAACTTTGGGTGATGGGAG+TGG 0.519583 2_2:+15737 Msa0228690:three_prime_UTR
AACTCCGGTGCGCTGTGCCA+CGG 0.520106 2_2:-15400 None:intergenic
TCTTGATGGTGTTGGTGATG+AGG 0.523044 2_2:+13927 Msa0228690:CDS
TAAAGCCAGTGGAACAATGC+TGG 0.530542 2_2:+13734 Msa0228690:CDS
GCTCGTGTCCAAACGGCCCA+CGG 0.531927 2_2:-13967 None:intergenic
TTTCTCCATCATATCACCAA+TGG 0.532359 2_2:-15310 None:intergenic
GTATCTTATTGGAGCTGGCA+TGG 0.533582 2_2:+14086 Msa0228690:CDS
TTGGTTCCAGTCCAAGCCCG+TGG 0.534499 2_2:+13950 Msa0228690:CDS
TTGGTGGTGGTAATAGGTGG+CGG 0.534962 2_2:-13588 None:intergenic
TAAGCTATTCGAGCACTTCG+CGG 0.535770 2_2:+15377 Msa0228690:CDS
AGTCCAAGCTCGTGTCCAAA+CGG 0.536753 2_2:-13974 None:intergenic
GTTTGCATTGGACAGGACCC+CGG 0.538725 2_2:+15066 Msa0228690:intron
GGATTAGAAGGTTGCAAGAG+AGG 0.539517 2_2:+15547 Msa0228690:CDS
AGTATGATCAATGGTCTTGA+TGG 0.539669 2_2:+13913 Msa0228690:CDS
GCTAGCCATTGGTGATATGA+TGG 0.539965 2_2:+15305 Msa0228690:CDS
CTTATTTGTATCTTTCTGGA+AGG 0.544191 2_2:+14031 Msa0228690:CDS
CCATCATACATAAGGTGTGC+CGG 0.547215 2_2:-15345 None:intergenic
ATAGGTGGCGGTGTGGTCGG+TGG 0.548279 2_2:-13576 None:intergenic
AAACAAGGAACTTTGGGTGA+TGG 0.549981 2_2:+15731 Msa0228690:three_prime_UTR
TTTCAAATCACTAGAAAACT+GGG 0.554024 2_2:+13687 Msa0228690:CDS
CGTCGAGAAGCTTCTAGAAG+TGG 0.554197 2_2:+15266 Msa0228690:CDS
TGTTCCACTGGCTTTAGTAG+TGG 0.556461 2_2:-13727 None:intergenic
CTCGTGTCCAAACGGCCCAC+GGG 0.556802 2_2:-13966 None:intergenic
ACGGCTCGCTTAGCGAAAGA+AGG 0.562132 2_2:+15165 Msa0228690:CDS
ACAGGAGCAATGCTAGCCAT+TGG 0.563098 2_2:+15294 Msa0228690:CDS
CTTGAAGGATTAACGGGTGA+GGG 0.586052 2_2:+15486 Msa0228690:CDS
TGTATACAAATCCCAAGTAT+GGG 0.587703 2_2:-15101 None:intergenic
ACGTATCAGAGTATGATCAA+TGG 0.591704 2_2:+13904 Msa0228690:CDS
ACAGCGCACCGGAGTTTACA+CGG 0.592150 2_2:+15407 Msa0228690:CDS
ATCATTGGCCGTGTAAACTC+CGG 0.595715 2_2:-15415 None:intergenic
GATGTTGTTACTTGTTTGCG+TGG 0.596003 2_2:+15833 Msa0228690:three_prime_UTR
TTAAGACACTTCCATTAACA+TGG 0.596362 2_2:+13533 Msa0228690:CDS
GATTAGAAGGTTGCAAGAGA+GGG 0.596681 2_2:+15548 Msa0228690:CDS
ACAGAAAACAACCCATACTT+GGG 0.596760 2_2:+15090 Msa0228690:CDS
CTTCTAGAAGTGGACCCCAC+AGG 0.596989 2_2:+15276 Msa0228690:CDS
ATCAATGGTCTTGATGGTGT+TGG 0.597547 2_2:+13919 Msa0228690:CDS
CAACTAAACTTGACCCCATG+TGG 0.598754 2_2:-15597 None:intergenic
TGAATTTACTGATCAGGTGA+AGG 0.600944 2_2:+13798 Msa0228690:CDS
GTTGGTTGGTGATATGGTGA+CGG 0.602166 2_2:+13867 Msa0228690:CDS
GCTTGAAGGATTAACGGGTG+AGG 0.603070 2_2:+15485 Msa0228690:CDS
TGGTTCCAGTCCAAGCCCGT+GGG 0.608947 2_2:+13951 Msa0228690:CDS
CGAGCAACATTTGTATCACA+CGG 0.621285 2_2:+15138 Msa0228690:CDS
ATTTGTATCACACGGAAACA+CGG 0.623224 2_2:+15146 Msa0228690:CDS
TTGATCATACTCTGATACGT+CGG 0.624523 2_2:-13901 None:intergenic
CAAATGGCAATGAAGGATCA+GGG 0.626451 2_2:-13774 None:intergenic
CAACAAAATACTCATCAGGA+AGG 0.632422 2_2:-13843 None:intergenic
GGTGGTAATAGGTGGCGGTG+TGG 0.634289 2_2:-13583 None:intergenic
TGGCTAGCATTGCTCCTGTG+GGG 0.636911 2_2:-15290 None:intergenic
CTCGTAAGATGAAACCACAT+GGG 0.643536 2_2:+15583 Msa0228690:CDS
CTGCTGAAGAAAATAGACAT+GGG 0.645814 2_2:+13995 Msa0228690:CDS
GCTCGTAAGATGAAACCACA+TGG 0.658763 2_2:+15582 Msa0228690:CDS
TGCTGAAGAAAATAGACATG+GGG 0.668352 2_2:+13996 Msa0228690:CDS
ACTGCTGAAGAAAATAGACA+TGG 0.671679 2_2:+13994 Msa0228690:CDS
TTTACCACTACTAAAGCCAG+TGG 0.671826 2_2:+13723 Msa0228690:CDS
ACTAATCTTGACACTAAACA+AGG 0.675822 2_2:+15716 Msa0228690:three_prime_UTR
GCTCGCTTAGCGAAAGAAGG+CGG 0.694123 2_2:+15168 Msa0228690:CDS
GGTCCTTCGTGCGATCACGA+AGG 0.703598 2_2:-13822 None:intergenic
GGATCAGGGAGAAAATTCTG+TGG 0.710660 2_2:-13760 None:intergenic
GGTCCTTCGTGATCGCACGA+AGG 0.710721 2_2:+13819 Msa0228690:CDS
TCGTAAGATGAAACCACATG+GGG 0.759922 2_2:+15584 Msa0228690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAATAATTAGATAAAT+TGG + chr2_2:14192-14211 Msa0228690:intron 10.0%
!! ATTAAAAATAAATCTATGAT+AGG + chr2_2:14546-14565 Msa0228690:intron 10.0%
!! TTAAAAATAAATCTATGATA+GGG + chr2_2:14547-14566 Msa0228690:intron 10.0%
!! CATATTATTAATTAAAGTGA+TGG + chr2_2:14229-14248 Msa0228690:intron 15.0%
!! AAAAAAAAAAAAAAAAACCG+AGG - chr2_2:14605-14624 None:intergenic 15.0%
!!! TTTTTTACTTTCTAACTAAA+TGG + chr2_2:15004-15023 Msa0228690:intron 15.0%
!! ATATGATCTATACTAGTTAA+TGG - chr2_2:14470-14489 None:intergenic 20.0%
!! TTGAAAAAATATAGAATCCT+CGG + chr2_2:14624-14643 Msa0228690:intron 20.0%
!!! AATGCTCTGTTTTTTTTTTT+TGG + chr2_2:14678-14697 Msa0228690:intron 20.0%
!!! AATTGATTTTTCATGTGTTA+CGG + chr2_2:14966-14985 Msa0228690:intron 20.0%
!!! TAGTTGGATTTTTAATAATG+AGG + chr2_2:15614-15633 Msa0228690:CDS 20.0%
!! AGTTTGAATATGCATTGTTT+TGG - chr2_2:13503-13522 None:intergenic 25.0%
!!! TTGAAAATTTCTAGCTTTTC+TGG - chr2_2:13673-13692 None:intergenic 25.0%
!! TTTTCAAATCACTAGAAAAC+TGG + chr2_2:13686-13705 Msa0228690:CDS 25.0%
! TTTCAAATCACTAGAAAACT+GGG + chr2_2:13687-13706 Msa0228690:CDS 25.0%
! TGATCAGTAAATTCATCAAA+TGG - chr2_2:13793-13812 None:intergenic 25.0%
! ATTTGATGAATTTACTGATC+AGG + chr2_2:13792-13811 Msa0228690:CDS 25.0%
!! TTATTTGTATCTTTCTGGAA+GGG + chr2_2:14032-14051 Msa0228690:CDS 25.0%
! AAAACTGTTCAGTATCTTAT+TGG + chr2_2:14075-14094 Msa0228690:CDS 25.0%
!! TGTACATTTTATTGCAATCT+AGG + chr2_2:14163-14182 Msa0228690:intron 25.0%
! ATCTATCTAAATAGAATCCT+CGG + chr2_2:14585-14604 Msa0228690:intron 25.0%
! CAAAACAGAACATTATGTTT+CGG - chr2_2:14705-14724 None:intergenic 25.0%
! TCATGTAACATAAAAGTGAT+GGG - chr2_2:14774-14793 None:intergenic 25.0%
! TTCATGTAACATAAAAGTGA+TGG - chr2_2:14775-14794 None:intergenic 25.0%
!! ACTTTTATGTTACATGAATG+AGG + chr2_2:14777-14796 Msa0228690:intron 25.0%
! ACTACTTAGAAGAAAAGTTT+CGG - chr2_2:14886-14905 None:intergenic 25.0%
!!! TAATTGATTTTTCACATGTG+TGG + chr2_2:14929-14948 Msa0228690:intron 25.0%
!!! AATTGATTTTTCACATGTGT+GGG + chr2_2:14930-14949 Msa0228690:intron 25.0%
!! GTAGACATTATTTTCAACTT+AGG - chr2_2:15036-15055 None:intergenic 25.0%
!!! TTTTGGAGTTTTTAATCGAA+CGG + chr2_2:15448-15467 Msa0228690:CDS 25.0%
!!! ATGTATTTTGACTCTAATGT+TGG + chr2_2:15788-15807 Msa0228690:three_prime_UTR 25.0%
! TTTTGGATTTGATACTAGCT+AGG - chr2_2:13486-13505 None:intergenic 30.0%
TTAAGACACTTCCATTAACA+TGG + chr2_2:13533-13552 Msa0228690:CDS 30.0%
! TTTGGAATGTTCCATGTTAA+TGG - chr2_2:13547-13566 None:intergenic 30.0%
!!! AAAATTTCTAGCTTTTCTGG+TGG - chr2_2:13670-13689 None:intergenic 30.0%
AATTCATCAAATGGCAATGA+AGG - chr2_2:13784-13803 None:intergenic 30.0%
AACACAACAAAATACTCATC+AGG - chr2_2:13850-13869 None:intergenic 30.0%
!! TGATGAGTATTTTGTTGTGT+TGG + chr2_2:13849-13868 Msa0228690:CDS 30.0%
AGGACTTATTTGTATCTTTC+TGG + chr2_2:14027-14046 Msa0228690:CDS 30.0%
CTTATTTGTATCTTTCTGGA+AGG + chr2_2:14031-14050 Msa0228690:CDS 30.0%
!! TTAAAGTGATGGATAGTAGT+AGG + chr2_2:14240-14259 Msa0228690:intron 30.0%
! GCAACATTGTAATTTGTAAG+AGG - chr2_2:14444-14463 None:intergenic 30.0%
ATGTAACATAAAAGTGATGG+GGG - chr2_2:14772-14791 None:intergenic 30.0%
CATGTAACATAAAAGTGATG+GGG - chr2_2:14773-14792 None:intergenic 30.0%
!! ACATTGCATTTTGTTTGCAT+TGG + chr2_2:15054-15073 Msa0228690:intron 30.0%
TGTATACAAATCCCAAGTAT+GGG - chr2_2:15104-15123 None:intergenic 30.0%
CAAAATATCAGCGTAATCAT+TGG - chr2_2:15433-15452 None:intergenic 30.0%
! CAATGATTACGCTGATATTT+TGG + chr2_2:15431-15450 Msa0228690:CDS 30.0%
ACTAATCTTGACACTAAACA+AGG + chr2_2:15716-15735 Msa0228690:three_prime_UTR 30.0%
! TGAAAAATAGTTGGGACTTT+TGG + chr2_2:15857-15876 Msa0228690:three_prime_UTR 30.0%
!!! GTTTTGGAGATTTTTAGCGA+CGG - chr2_2:13637-13656 None:intergenic 35.0%
TGAATTTACTGATCAGGTGA+AGG + chr2_2:13798-13817 Msa0228690:CDS 35.0%
CAACAAAATACTCATCAGGA+AGG - chr2_2:13846-13865 None:intergenic 35.0%
!! GAGTATTTTGTTGTGTTGGT+TGG + chr2_2:13853-13872 Msa0228690:CDS 35.0%
TTGATCATACTCTGATACGT+CGG - chr2_2:13904-13923 None:intergenic 35.0%
ACGTATCAGAGTATGATCAA+TGG + chr2_2:13904-13923 Msa0228690:CDS 35.0%
! AGTATGATCAATGGTCTTGA+TGG + chr2_2:13913-13932 Msa0228690:CDS 35.0%
ACTGCTGAAGAAAATAGACA+TGG + chr2_2:13994-14013 Msa0228690:CDS 35.0%
CTGCTGAAGAAAATAGACAT+GGG + chr2_2:13995-14014 Msa0228690:CDS 35.0%
TGCTGAAGAAAATAGACATG+GGG + chr2_2:13996-14015 Msa0228690:CDS 35.0%
ACAGAAAACAACCCATACTT+GGG + chr2_2:15090-15109 Msa0228690:CDS 35.0%
GTGTATACAAATCCCAAGTA+TGG - chr2_2:15105-15124 None:intergenic 35.0%
ATTTGTATCACACGGAAACA+CGG + chr2_2:15146-15165 Msa0228690:CDS 35.0%
TTTCTCCATCATATCACCAA+TGG - chr2_2:15313-15332 None:intergenic 35.0%
GGAGAAAAAAATCACAATGC+CGG + chr2_2:15326-15345 Msa0228690:CDS 35.0%
GGAAAAGCTTGAAGGATTAA+CGG + chr2_2:15479-15498 Msa0228690:CDS 35.0%
GAAAAGCTTGAAGGATTAAC+GGG + chr2_2:15480-15499 Msa0228690:CDS 35.0%
AGACTAGAGATTAAGAGCTA+AGG + chr2_2:15676-15695 Msa0228690:three_prime_UTR 35.0%
GACTAGAGATTAAGAGCTAA+GGG + chr2_2:15677-15696 Msa0228690:three_prime_UTR 35.0%
TGACACTAAACAAGGAACTT+TGG + chr2_2:15724-15743 Msa0228690:three_prime_UTR 35.0%
GACACTAAACAAGGAACTTT+GGG + chr2_2:15725-15744 Msa0228690:three_prime_UTR 35.0%
!! TTTTGACTCTAATGTTGGAG+AGG + chr2_2:15793-15812 Msa0228690:three_prime_UTR 35.0%
TTGCGTGGATGAAAAATAGT+TGG + chr2_2:15848-15867 Msa0228690:three_prime_UTR 35.0%
TGCGTGGATGAAAAATAGTT+GGG + chr2_2:15849-15868 Msa0228690:three_prime_UTR 35.0%
!!! ATTTTGGTGGTGGTAATAGG+TGG - chr2_2:13594-13613 None:intergenic 40.0%
!!! TGGATTTTGGTGGTGGTAAT+AGG - chr2_2:13597-13616 None:intergenic 40.0%
TTTACCACTACTAAAGCCAG+TGG + chr2_2:13723-13742 Msa0228690:CDS 40.0%
CAAATGGCAATGAAGGATCA+GGG - chr2_2:13777-13796 None:intergenic 40.0%
TCAAATGGCAATGAAGGATC+AGG - chr2_2:13778-13797 None:intergenic 40.0%
!! TGTTGTGTTGGTTGGTGATA+TGG + chr2_2:13861-13880 Msa0228690:CDS 40.0%
!! ATCAATGGTCTTGATGGTGT+TGG + chr2_2:13919-13938 Msa0228690:CDS 40.0%
! ATAGACATGGGGATTTGCTT+AGG + chr2_2:14007-14026 Msa0228690:CDS 40.0%
!! TCTTTCTGGAAGGGTTGATA+TGG + chr2_2:14041-14060 Msa0228690:CDS 40.0%
GTTCAGTATCTTATTGGAGC+TGG + chr2_2:14081-14100 Msa0228690:CDS 40.0%
!! GGATAGTAGTAGGAGTGTTT+AGG + chr2_2:14250-14269 Msa0228690:intron 40.0%
!! TAGTAGTAGGAGTGTTTAGG+TGG + chr2_2:14253-14272 Msa0228690:intron 40.0%
!! GCATTTTGTTTGCATTGGAC+AGG + chr2_2:15059-15078 Msa0228690:intron 40.0%
GACAGAAAACAACCCATACT+TGG + chr2_2:15089-15108 Msa0228690:CDS 40.0%
ATACAAATGTTGCTCGCTCT+TGG - chr2_2:15134-15153 None:intergenic 40.0%
CGAGCAACATTTGTATCACA+CGG + chr2_2:15138-15157 Msa0228690:CDS 40.0%
! GTTCTCATGACGCTTTTCAT+CGG - chr2_2:15232-15251 None:intergenic 40.0%
! GGTCTTGTCCATCATACATA+AGG - chr2_2:15356-15375 None:intergenic 40.0%
! TGGAGTTTTTAATCGAACGG+TGG + chr2_2:15451-15470 Msa0228690:CDS 40.0%
TTTAATCGAACGGTGGAGAT+TGG + chr2_2:15458-15477 Msa0228690:CDS 40.0%
TGGAGATTGGAAAAGCTTGA+AGG + chr2_2:15471-15490 Msa0228690:CDS 40.0%
! ATTAACGGGTGAGGGAAAAA+GGG + chr2_2:15494-15513 Msa0228690:CDS 40.0%
TCTTGCAACCTTCTAATCCT+AGG - chr2_2:15546-15565 None:intergenic 40.0%
GATTAGAAGGTTGCAAGAGA+GGG + chr2_2:15548-15567 Msa0228690:CDS 40.0%
CTCGTAAGATGAAACCACAT+GGG + chr2_2:15583-15602 Msa0228690:CDS 40.0%
TCGTAAGATGAAACCACATG+GGG + chr2_2:15584-15603 Msa0228690:CDS 40.0%
AAACAAGGAACTTTGGGTGA+TGG + chr2_2:15731-15750 Msa0228690:three_prime_UTR 40.0%
AACAAGGAACTTTGGGTGAT+GGG + chr2_2:15732-15751 Msa0228690:three_prime_UTR 40.0%
CTCTAATGTTGGAGAGGATA+TGG + chr2_2:15799-15818 Msa0228690:three_prime_UTR 40.0%
! GATGTTGTTACTTGTTTGCG+TGG + chr2_2:15833-15852 Msa0228690:three_prime_UTR 40.0%
!!! TTGGAGATTTTTAGCGACGG+TGG - chr2_2:13634-13653 None:intergenic 45.0%
!! CTTTTCTGGTGGCATTGAGT+GGG - chr2_2:13659-13678 None:intergenic 45.0%
!! TGTTCCACTGGCTTTAGTAG+TGG - chr2_2:13730-13749 None:intergenic 45.0%
TAAAGCCAGTGGAACAATGC+TGG + chr2_2:13734-13753 Msa0228690:CDS 45.0%
GGATCAGGGAGAAAATTCTG+TGG - chr2_2:13763-13782 None:intergenic 45.0%
!! GTTGGTTGGTGATATGGTGA+CGG + chr2_2:13867-13886 Msa0228690:CDS 45.0%
!! TCTTGATGGTGTTGGTGATG+AGG + chr2_2:13927-13946 Msa0228690:CDS 45.0%
GTATCTTATTGGAGCTGGCA+TGG + chr2_2:14086-14105 Msa0228690:CDS 45.0%
CAACATATGCGATCAAACCG+AGG - chr2_2:14644-14663 None:intergenic 45.0%
!! TATGGGTTGTTTTCTGTCCC+GGG - chr2_2:15087-15106 None:intergenic 45.0%
!! GTATGGGTTGTTTTCTGTCC+CGG - chr2_2:15088-15107 None:intergenic 45.0%
! GCTAGCCATTGGTGATATGA+TGG + chr2_2:15305-15324 Msa0228690:CDS 45.0%
CCATCATACATAAGGTGTGC+CGG - chr2_2:15348-15367 None:intergenic 45.0%
! CGAAGTGCTCGAATAGCTTA+GGG - chr2_2:15377-15396 None:intergenic 45.0%
TAAGCTATTCGAGCACTTCG+CGG + chr2_2:15377-15396 Msa0228690:CDS 45.0%
ATCATTGGCCGTGTAAACTC+CGG - chr2_2:15418-15437 None:intergenic 45.0%
! CTTGAAGGATTAACGGGTGA+GGG + chr2_2:15486-15505 Msa0228690:CDS 45.0%
! GATTAACGGGTGAGGGAAAA+AGG + chr2_2:15493-15512 Msa0228690:CDS 45.0%
GGTTAGCACCTAGGATTAGA+AGG + chr2_2:15535-15554 Msa0228690:CDS 45.0%
GGATTAGAAGGTTGCAAGAG+AGG + chr2_2:15547-15566 Msa0228690:CDS 45.0%
GCTCGTAAGATGAAACCACA+TGG + chr2_2:15582-15601 Msa0228690:CDS 45.0%
CAACTAAACTTGACCCCATG+TGG - chr2_2:15600-15619 None:intergenic 45.0%
CACATGGGGTCAAGTTTAGT+TGG + chr2_2:15598-15617 Msa0228690:CDS 45.0%
!!! TTTAATTTCATTTAATATTT+TGG + chr2_2:14129-14148 Msa0228690:intron 5.0%
!!! AAAAGATTTATTTTTTTTTA+GGG - chr2_2:14517-14536 None:intergenic 5.0%
!!! AAAAAGATTTATTTTTTTTT+AGG - chr2_2:14518-14537 None:intergenic 5.0%
!!! GGTCGGTGGTTGATGGTTTT+TGG - chr2_2:13565-13584 None:intergenic 50.0%
!! TTGGTGGTGGTAATAGGTGG+CGG - chr2_2:13591-13610 None:intergenic 50.0%
!!! GTGATGGTGGATTTTGGTGG+TGG - chr2_2:13604-13623 None:intergenic 50.0%
!!! GTGGTGATGGTGGATTTTGG+TGG - chr2_2:13607-13626 None:intergenic 50.0%
!!! GTGGTGGTGATGGTGGATTT+TGG - chr2_2:13610-13629 None:intergenic 50.0%
!!! GATTTTTAGCGACGGTGGTG+TGG - chr2_2:13629-13648 None:intergenic 50.0%
!! TGGTGGCATTGAGTGGGTTT+TGG - chr2_2:13653-13672 None:intergenic 50.0%
!! GCTTTTCTGGTGGCATTGAG+TGG - chr2_2:13660-13679 None:intergenic 50.0%
!! GATGGTGTTGGTGATGAGGT+TGG + chr2_2:13931-13950 Msa0228690:CDS 50.0%
AGTCCAAGCTCGTGTCCAAA+CGG - chr2_2:13977-13996 None:intergenic 50.0%
!! ATGGGTTGTTTTCTGTCCCG+GGG - chr2_2:15086-15105 None:intergenic 50.0%
! CGTCGAGAAGCTTCTAGAAG+TGG + chr2_2:15266-15285 Msa0228690:CDS 50.0%
ATGGCTAGCATTGCTCCTGT+GGG - chr2_2:15294-15313 None:intergenic 50.0%
AATGGCTAGCATTGCTCCTG+TGG - chr2_2:15295-15314 None:intergenic 50.0%
ACAGGAGCAATGCTAGCCAT+TGG + chr2_2:15294-15313 Msa0228690:CDS 50.0%
CCGGCACACCTTATGTATGA+TGG + chr2_2:15345-15364 Msa0228690:CDS 50.0%
! GCGAAGTGCTCGAATAGCTT+AGG - chr2_2:15378-15397 None:intergenic 50.0%
! GCTTGAAGGATTAACGGGTG+AGG + chr2_2:15485-15504 Msa0228690:CDS 50.0%
!! TTGTGTGTGGGTTAGCACCT+AGG + chr2_2:15526-15545 Msa0228690:CDS 50.0%
! GTAATAGGTGGCGGTGTGGT+CGG - chr2_2:13582-13601 None:intergenic 55.0%
! TTTTAGCGACGGTGGTGTGG+TGG - chr2_2:13626-13645 None:intergenic 55.0%
GTTTGCATTGGACAGGACCC+CGG + chr2_2:15066-15085 Msa0228690:intron 55.0%
TTTGCATTGGACAGGACCCC+GGG + chr2_2:15067-15086 Msa0228690:intron 55.0%
ACGGCTCGCTTAGCGAAAGA+AGG + chr2_2:15165-15184 Msa0228690:CDS 55.0%
GCTCGCTTAGCGAAAGAAGG+CGG + chr2_2:15168-15187 Msa0228690:CDS 55.0%
!! ACGCTTTTCATCGGCTGCGA+TGG - chr2_2:15223-15242 None:intergenic 55.0%
! CTTCTAGAAGTGGACCCCAC+AGG + chr2_2:15276-15295 Msa0228690:CDS 55.0%
TGGCTAGCATTGCTCCTGTG+GGG - chr2_2:15293-15312 None:intergenic 55.0%
ACAGCGCACCGGAGTTTACA+CGG + chr2_2:15407-15426 Msa0228690:CDS 55.0%
! AGGGCGCAGGATTTTGTGTG+TGG + chr2_2:15513-15532 Msa0228690:CDS 55.0%
! GGGCGCAGGATTTTGTGTGT+GGG + chr2_2:15514-15533 Msa0228690:CDS 55.0%
!! GGAACTTTGGGTGATGGGAG+TGG + chr2_2:15737-15756 Msa0228690:three_prime_UTR 55.0%
! GGTGGTAATAGGTGGCGGTG+TGG - chr2_2:13586-13605 None:intergenic 60.0%
GGCTGCCAGCATTGTTCCAC+TGG - chr2_2:13742-13761 None:intergenic 60.0%
GGTCCTTCGTGATCGCACGA+AGG + chr2_2:13819-13838 Msa0228690:CDS 60.0%
GGTCCTTCGTGCGATCACGA+AGG - chr2_2:13825-13844 None:intergenic 60.0%
TTGGTTCCAGTCCAAGCCCG+TGG + chr2_2:13950-13969 Msa0228690:CDS 60.0%
TGGTTCCAGTCCAAGCCCGT+GGG + chr2_2:13951-13970 Msa0228690:CDS 60.0%
CCACATATGCGCGCAAGCAC+TGG - chr2_2:15198-15217 None:intergenic 60.0%
CCAGTGCTTGCGCGCATATG+TGG + chr2_2:15195-15214 Msa0228690:CDS 60.0%
GCGGTGTGGTCGGTGGTTGA+TGG - chr2_2:13572-13591 None:intergenic 65.0%
! ATAGGTGGCGGTGTGGTCGG+TGG - chr2_2:13579-13598 None:intergenic 65.0%
!! CGGTGGTGTGGTGGTGATGG+TGG - chr2_2:13617-13636 None:intergenic 65.0%
! CGACGGTGGTGTGGTGGTGA+TGG - chr2_2:13620-13639 None:intergenic 65.0%
CTCGTGTCCAAACGGCCCAC+GGG - chr2_2:13969-13988 None:intergenic 65.0%
GCTCGTGTCCAAACGGCCCA+CGG - chr2_2:13970-13989 None:intergenic 65.0%
GGGCCGTTTGGACACGAGCT+TGG + chr2_2:13971-13990 Msa0228690:CDS 65.0%
ATTCGAGCACTTCGCGGCCG+TGG + chr2_2:15383-15402 Msa0228690:CDS 65.0%
AACTCCGGTGCGCTGTGCCA+CGG - chr2_2:15403-15422 None:intergenic 65.0%
! GGGTGAGGGAAAAAGGGCGC+AGG + chr2_2:15500-15519 Msa0228690:CDS 65.0%
AACGGCCCACGGGCTTGGAC+TGG - chr2_2:13959-13978 None:intergenic 70.0%
GTCCAAGCCCGTGGGCCGTT+TGG + chr2_2:13959-13978 Msa0228690:CDS 70.0%
GTCCAAACGGCCCACGGGCT+TGG - chr2_2:13964-13983 None:intergenic 70.0%
! GCGGCCGTGGCACAGCGCAC+CGG + chr2_2:15396-15415 Msa0228690:CDS 80.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 13468 15977 13468 ID=Msa0228690;Name=Msa0228690
chr2_2 mRNA 13468 15977 13468 ID=Msa0228690-mRNA-1;Parent=Msa0228690;Name=Msa0228690-mRNA-1;_AED=0.03;_eAED=0.03;_QI=40|1|1|1|1|1|2|330|388
chr2_2 exon 13468 14107 13468 ID=Msa0228690-mRNA-1:exon:2;Parent=Msa0228690-mRNA-1
chr2_2 exon 15081 15977 15081 ID=Msa0228690-mRNA-1:exon:3;Parent=Msa0228690-mRNA-1
chr2_2 five_prime_UTR 13468 13507 13468 ID=Msa0228690-mRNA-1:five_prime_utr;Parent=Msa0228690-mRNA-1
chr2_2 CDS 13508 14107 13508 ID=Msa0228690-mRNA-1:cds;Parent=Msa0228690-mRNA-1
chr2_2 CDS 15081 15647 15081 ID=Msa0228690-mRNA-1:cds;Parent=Msa0228690-mRNA-1
chr2_2 three_prime_UTR 15648 15977 15648 ID=Msa0228690-mRNA-1:three_prime_utr;Parent=Msa0228690-mRNA-1
Gene Sequence

>Msa0228690

ATGCATATTCAAACTTGTCACTCAATTAAGACACTTCCATTAACATGGAACATTCCAAAAACCATCAACCACCGACCACACCGCCACCTATTACCACCACCAAAATCCACCATCACCACCACACCACCGTCGCTAAAAATCTCCAAAACCCACTCAATGCCACCAGAAAAGCTAGAAATTTTCAAATCACTAGAAAACTGGGCTTCTGAGTCTGTTTTACCACTACTAAAGCCAGTGGAACAATGCTGGCAGCCACAGAATTTTCTCCCTGATCCTTCATTGCCATTTGATGAATTTACTGATCAGGTGAAGGTCCTTCGTGATCGCACGAAGGACCTTCCTGATGAGTATTTTGTTGTGTTGGTTGGTGATATGGTGACGGAAGACGCACTTCCGACGTATCAGAGTATGATCAATGGTCTTGATGGTGTTGGTGATGAGGTTGGTTCCAGTCCAAGCCCGTGGGCCGTTTGGACACGAGCTTGGACTGCTGAAGAAAATAGACATGGGGATTTGCTTAGGACTTATTTGTATCTTTCTGGAAGGGTTGATATGGAGATGATTGAGAAAACTGTTCAGTATCTTATTGGAGCTGGCATGGACCCCGGGACAGAAAACAACCCATACTTGGGATTTGTATACACATCATTCCAAGAGCGAGCAACATTTGTATCACACGGAAACACGGCTCGCTTAGCGAAAGAAGGCGGTGATCCAGTGCTTGCGCGCATATGTGGCACCATCGCAGCCGATGAAAAGCGTCATGAGAACGCATACTCAAAAATCGTCGAGAAGCTTCTAGAAGTGGACCCCACAGGAGCAATGCTAGCCATTGGTGATATGATGGAGAAAAAAATCACAATGCCGGCACACCTTATGTATGATGGACAAGACCCTAAGCTATTCGAGCACTTCGCGGCCGTGGCACAGCGCACCGGAGTTTACACGGCCAATGATTACGCTGATATTTTGGAGTTTTTAATCGAACGGTGGAGATTGGAAAAGCTTGAAGGATTAACGGGTGAGGGAAAAAGGGCGCAGGATTTTGTGTGTGGGTTAGCACCTAGGATTAGAAGGTTGCAAGAGAGGGCTGATGCACGTGCTCGTAAGATGAAACCACATGGGGTCAAGTTTAGTTGGATTTTTAATAATGAGGTTGTTTTGTGA
Protein sequence

>Msa0228690

MHIQTCHSIKTLPLTWNIPKTINHRPHRHLLPPPKSTITTTPPSLKISKTHSMPPEKLEIFKSLENWASESVLPLLKPVEQCWQPQNFLPDPSLPFDEFTDQVKVLRDRTKDLPDEYFVVLVGDMVTEDALPTYQSMINGLDGVGDEVGSSPSPWAVWTRAWTAEENRHGDLLRTYLYLSGRVDMEMIEKTVQYLIGAGMDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKEGGDPVLARICGTIAADEKRHENAYSKIVEKLLEVDPTGAMLAIGDMMEKKITMPAHLMYDGQDPKLFEHFAAVAQRTGVYTANDYADILEFLIERWRLEKLEGLTGEGKRAQDFVCGLAPRIRRLQERADARARKMKPHGVKFSWIFNNEVVL*