AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0902270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0902270 MtrunA17_Chr1g0159321 97.692 390 7 1 1 388 1 390 0.0 791
Msa0902270 MtrunA17_Chr3g0126011 74.709 344 84 1 45 388 26 366 0.0 549
Msa0902270 MtrunA17_Chr3g0125981 71.191 361 94 2 26 386 18 368 0.0 536
Msa0902270 MtrunA17_Chr3g0125991 71.229 358 98 2 33 388 12 366 0.0 535
Msa0902270 MtrunA17_Chr3g0125781 70.857 350 99 1 39 388 23 369 0.0 533
Msa0902270 MtrunA17_Chr5g0406961 70.201 349 100 2 40 388 100 444 0.0 527
Msa0902270 MtrunA17_Chr4g0045661 70.201 349 100 2 40 388 49 393 0.0 525
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0902270 AT1G43800.1 77.654 358 76 3 33 386 32 389 0.0 587
Msa0902270 AT3G02630.1 70.145 345 100 2 42 386 53 394 0.0 520
Msa0902270 AT2G43710.2 68.768 349 105 2 40 388 57 401 0.0 511
Msa0902270 AT2G43710.1 68.768 349 105 2 40 388 57 401 0.0 511
Msa0902270 AT5G16240.1 67.536 345 109 2 42 386 51 392 9.03e-178 500
Msa0902270 AT5G16230.1 67.353 340 109 2 50 388 63 401 1.04e-173 490
Msa0902270 AT3G02610.2 68.222 343 103 4 50 388 73 413 1.08e-172 488
Msa0902270 AT3G02610.1 68.222 343 103 4 50 388 71 411 1.16e-172 488
Msa0902270 AT3G02620.1 63.636 374 120 6 26 388 35 403 3.98e-170 481
Msa0902270 AT3G02620.2 63.636 374 120 6 26 388 38 406 4.72e-170 481
Msa0902270 AT5G16230.2 66.871 326 106 2 64 388 1 325 5.22e-165 465
Msa0902270 AT3G02620.3 66.869 329 103 4 64 388 1 327 5.89e-162 457

Find 118 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGACTTATTTGTATCTTTC+TGG 0.182339 6_1:+100261075 Msa0902270:CDS
GAGACTAGAGATTAAGAGTT+AGG 0.239531 6_1:+100262747 Msa0902270:three_prime_UTR
GTGTATACAAATCCCAAGTA+TGG 0.240289 6_1:-100262183 None:intergenic
GATTAACGGGTGAGGGAAAA+AGG 0.240677 6_1:+100262574 Msa0902270:CDS
TTTGGAATGTTCCATGTTAA+TGG 0.243926 6_1:-100260592 None:intergenic
TGTTGTGTTGGTTGGTGATA+TGG 0.248245 6_1:+100260909 Msa0902270:CDS
GGTCGGTGGTTGATGGATTT+TGG 0.248989 6_1:-100260610 None:intergenic
GTGGTGGTGACGGTGGATTT+TGG 0.310655 6_1:-100260655 None:intergenic
AGTTTGAATATGCATTGTTT+TGG 0.317713 6_1:-100260548 None:intergenic
TGGTGGCATTGAGTGGGTTT+TGG 0.321308 6_1:-100260698 None:intergenic
GTCCAAGCCCGTGGGCCGTT+TGG 0.332806 6_1:+100261007 Msa0902270:CDS
TCTTTCTGGAAGGGTTGATA+TGG 0.335225 6_1:+100261089 Msa0902270:CDS
GTCCAAACGGCCCACGGGCT+TGG 0.340343 6_1:-100261009 None:intergenic
GACACTAAACAAGGAACTTT+GGG 0.350550 6_1:+100262796 Msa0902270:three_prime_UTR
GTCTAATGTTGGAGAGGATA+TGG 0.373446 6_1:+100262870 Msa0902270:three_prime_UTR
ACGTATCAGAGTATGATTAA+TGG 0.377224 6_1:+100260952 Msa0902270:CDS
CAAGATATCCGCGTAATCAT+TGG 0.384695 6_1:-100262511 None:intergenic
GGCTGCCAGCATTGTTCCAC+TGG 0.386228 6_1:-100260787 None:intergenic
TGACACTAAACAAGGAACTT+TGG 0.390464 6_1:+100262795 Msa0902270:three_prime_UTR
CAATGATTACGCGGATATCT+TGG 0.392773 6_1:+100262512 Msa0902270:CDS
GGGCCGTTTGGACACGGGCT+TGG 0.396447 6_1:+100261019 Msa0902270:CDS
AACGGCCCACGGGCTTGGAC+TGG 0.398332 6_1:-100261004 None:intergenic
CACATGGGGTCAAGTTTAGT+TGG 0.400607 6_1:+100262679 Msa0902270:CDS
ATACAAATGTTGCTCGCTCT+TGG 0.405320 6_1:-100262212 None:intergenic
CGTCGAGAAGCTTCTAGAAA+TGG 0.405862 6_1:+100262347 Msa0902270:CDS
GCGATGTGGTCGGTGGTTGA+TGG 0.406929 6_1:-100260617 None:intergenic
TTTAATCGAACGGTGGAGAT+TGG 0.410251 6_1:+100262539 Msa0902270:CDS
GACAGAAAACAACCCATACT+TGG 0.410965 6_1:+100262170 Msa0902270:CDS
GGTTAGCACCTAGGATTAGA+AGG 0.411387 6_1:+100262616 Msa0902270:CDS
GATGGTGTTGGTGATGAGAT+TGG 0.415369 6_1:+100260979 Msa0902270:CDS
ATTTGATGAATTTACTGATC+AGG 0.419139 6_1:+100260840 Msa0902270:CDS
TCAAATGGCAATGAAGGATC+AGG 0.420107 6_1:-100260823 None:intergenic
AGACTAGAGATTAAGAGTTA+GGG 0.423729 6_1:+100262748 Msa0902270:three_prime_UTR
GCGAAGTGCTCGAATAGCTT+AGG 0.429820 6_1:-100262456 None:intergenic
GGAGAAGCTTGAAGGATTAA+CGG 0.433519 6_1:+100262560 Msa0902270:CDS
GCCCGTGGGCCGTTTGGACA+CGG 0.438549 6_1:+100261013 Msa0902270:CDS
TTTGCATTGGACAGGACCCC+GGG 0.454311 6_1:+100262148 Msa0902270:intron
TGATCAGTAAATTCATCAAA+TGG 0.459663 6_1:-100260838 None:intergenic
ATAGACATGGGGATTTGCTT+AGG 0.460553 6_1:+100261055 Msa0902270:CDS
AAAACTGTTCAGTATCTCAT+TGG 0.460584 6_1:+100261123 Msa0902270:CDS
AACAAGGAACTTTGGGTAGT+GGG 0.472377 6_1:+100262803 Msa0902270:three_prime_UTR
CCACATATGCGCGCAAGCAC+TGG 0.472837 6_1:-100262276 None:intergenic
AATTCATCAAATGGCAATGA+AGG 0.478762 6_1:-100260829 None:intergenic
CGAAGTGCTCGAATAGCTTA+GGG 0.481594 6_1:-100262455 None:intergenic
TTGTGTGTGGGTTAGCACCT+AGG 0.482074 6_1:+100262607 Msa0902270:CDS
GAGAAGCTTGAAGGATTAAC+GGG 0.482203 6_1:+100262561 Msa0902270:CDS
TGGAGATTGGAGAAGCTTGA+AGG 0.484791 6_1:+100262552 Msa0902270:CDS
CCAGTGCTTGCGCGCATATG+TGG 0.484813 6_1:+100262276 Msa0902270:CDS
ATTCGAGCACTTCGCGGCTG+TGG 0.485856 6_1:+100262464 Msa0902270:CDS
TCTTGCAACCTTCTAATCCT+AGG 0.486803 6_1:-100262624 None:intergenic
GCGGCTGTGGCACAGCGCAC+CGG 0.489252 6_1:+100262477 Msa0902270:CDS
AACACAACAAAATACTCATC+AGG 0.489365 6_1:-100260895 None:intergenic
CCGGCACACCTTATGTATGA+TGG 0.493314 6_1:+100262426 Msa0902270:CDS
GGTCTTGTCCATCATACATA+AGG 0.494290 6_1:-100262434 None:intergenic
TTATTTGTATCTTTCTGGAA+GGG 0.495330 6_1:+100261080 Msa0902270:CDS
AATGGCTAGCATTGCTCCCG+TGG 0.498130 6_1:-100262373 None:intergenic
GCCCGTGTCCAAACGGCCCA+CGG 0.499533 6_1:-100261015 None:intergenic
GATGTTGTTACTTGTTTGTG+TGG 0.503166 6_1:+100262904 Msa0902270:three_prime_UTR
CCCGTGGGCCGTTTGGACAC+GGG 0.513039 6_1:+100261014 Msa0902270:CDS
ATGGCTAGCATTGCTCCCGT+GGG 0.517421 6_1:-100262372 None:intergenic
CGACGGTGGTGTGGTGGTGA+CGG 0.521246 6_1:-100260665 None:intergenic
AGTCCAAGCCCGTGTCCAAA+CGG 0.523809 6_1:-100261022 None:intergenic
AGTATGATTAATGGTCTTGA+TGG 0.523991 6_1:+100260961 Msa0902270:CDS
TAAAGCCAGTGGAACAATGC+TGG 0.530542 6_1:+100260782 Msa0902270:CDS
TTTCTCCATCATATCACCAA+TGG 0.532359 6_1:-100262391 None:intergenic
GTAATAGGTGGCGATGTGGT+CGG 0.534786 6_1:-100260627 None:intergenic
ACGGGAGCAATGCTAGCCAT+TGG 0.537374 6_1:+100262375 Msa0902270:CDS
GTTTGCATTGGACAGGACCC+CGG 0.538725 6_1:+100262147 Msa0902270:intron
GCTAGCCATTGGTGATATGA+TGG 0.539965 6_1:+100262386 Msa0902270:CDS
TTGGTTCCAGTCCAAGCCCG+TGG 0.541070 6_1:+100260998 Msa0902270:CDS
ACAAGGAACTTTGGGTAGTG+GGG 0.541960 6_1:+100262804 Msa0902270:three_prime_UTR
CTTATTTGTATCTTTCTGGA+AGG 0.544191 6_1:+100261079 Msa0902270:CDS
CCATCATACATAAGGTGTGC+CGG 0.547215 6_1:-100262426 None:intergenic
GTTCAGTATCTCATTGGAGC+TGG 0.553993 6_1:+100261129 Msa0902270:CDS
TTTCAAATCACTAGAAAACT+GGG 0.554024 6_1:+100260735 Msa0902270:CDS
TAAGCTATTCGAGCACTTCG+CGG 0.554539 6_1:+100262458 Msa0902270:CDS
CCCGTGTCCAAACGGCCCAC+GGG 0.555548 6_1:-100261014 None:intergenic
GTATCTCATTGGAGCTGGCA+TGG 0.555720 6_1:+100261134 Msa0902270:CDS
TGTTCCACTGGCTTTAGTAG+TGG 0.556461 6_1:-100260775 None:intergenic
ACGGCTCGCTTAGCGAAAGA+TGG 0.564465 6_1:+100262246 Msa0902270:CDS
AAACAAGGAACTTTGGGTAG+TGG 0.574143 6_1:+100262802 Msa0902270:three_prime_UTR
CGGTGGTGTGGTGGTGACGG+TGG 0.585453 6_1:-100260662 None:intergenic
ATTAATGGTCTTGATGGTGT+TGG 0.585459 6_1:+100260967 Msa0902270:CDS
GCTTGAAGGATTAACGGGTG+AGG 0.586573 6_1:+100262566 Msa0902270:CDS
TGTATACAAATCCCAAGTAT+GGG 0.587703 6_1:-100262182 None:intergenic
ACAGCGCACCGGAGTTTACA+CGG 0.592150 6_1:+100262488 Msa0902270:CDS
ATCATTGGCCGTGTAAACTC+CGG 0.595715 6_1:-100262496 None:intergenic
CTTGAAGGATTAACGGGTGA+GGG 0.596019 6_1:+100262567 Msa0902270:CDS
TTAAGACACTTCCATTAACA+TGG 0.596362 6_1:+100260581 Msa0902270:CDS
ACAGAAAACAACCCATACTT+GGG 0.596760 6_1:+100262171 Msa0902270:CDS
CAACTAAACTTGACCCCATG+TGG 0.598754 6_1:-100262678 None:intergenic
TGAATTTACTGATCAGGTGA+AGG 0.600944 6_1:+100260846 Msa0902270:CDS
GTTGGTTGGTGATATGGTGA+CGG 0.602166 6_1:+100260915 Msa0902270:CDS
GCTTCTAGAAATGGACCCCA+CGG 0.602940 6_1:+100262356 Msa0902270:CDS
CGAGCAACATTTGTATCACA+CGG 0.621285 6_1:+100262219 Msa0902270:CDS
TGGTTCCAGTCCAAGCCCGT+GGG 0.621792 6_1:+100260999 Msa0902270:CDS
ATTTGTATCACACGGAAACA+CGG 0.623224 6_1:+100262227 Msa0902270:CDS
TTAATCATACTCTGATACGT+CGG 0.624523 6_1:-100260949 None:intergenic
CAAATGGCAATGAAGGATCA+GGG 0.626451 6_1:-100260822 None:intergenic
CAACAAAATACTCATCAGGA+AGG 0.632422 6_1:-100260891 None:intergenic
CTTCTAGAAATGGACCCCAC+GGG 0.638314 6_1:+100262357 Msa0902270:CDS
ATAGGTGGCGATGTGGTCGG+TGG 0.643329 6_1:-100260624 None:intergenic
CTCGTAAGATGAAACCACAT+GGG 0.643536 6_1:+100262664 Msa0902270:CDS
CAAGGAACTTTGGGTAGTGG+GGG 0.645435 6_1:+100262805 Msa0902270:three_prime_UTR
CTGCTGAAGAAAATAGACAT+GGG 0.645814 6_1:+100261043 Msa0902270:CDS
GCTCGCTTAGCGAAAGATGG+CGG 0.654478 6_1:+100262249 Msa0902270:CDS
GCTCGTAAGATGAAACCACA+TGG 0.658763 6_1:+100262663 Msa0902270:CDS
GGTGGTAATAGGTGGCGATG+TGG 0.665415 6_1:-100260631 None:intergenic
TGCTGAAGAAAATAGACATG+GGG 0.668352 6_1:+100261044 Msa0902270:CDS
TGGCTAGCATTGCTCCCGTG+GGG 0.669064 6_1:-100262371 None:intergenic
ACTGCTGAAGAAAATAGACA+TGG 0.671679 6_1:+100261042 Msa0902270:CDS
TTTACCACTACTAAAGCCAG+TGG 0.671826 6_1:+100260771 Msa0902270:CDS
ACTAATCTTGACACTAAACA+AGG 0.675822 6_1:+100262787 Msa0902270:three_prime_UTR
GGTCCTTCGTGCGATCACGA+AGG 0.703598 6_1:-100260870 None:intergenic
GGATCAGGGAGAAAATTCTG+TGG 0.710660 6_1:-100260808 None:intergenic
GGTCCTTCGTGATCGCACGA+AGG 0.710721 6_1:+100260867 Msa0902270:CDS
TTACACGGCCAATGATTACG+CGG 0.711680 6_1:+100262503 Msa0902270:CDS
TCGTAAGATGAAACCACATG+GGG 0.759922 6_1:+100262665 Msa0902270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAATAATTAGATAAAT+TGG + chr6_1:100261240-100261259 Msa0902270:intron 10.0%
!! TTCAAAAATAAATCTATAAT+AGG + chr6_1:100261551-100261570 Msa0902270:intron 10.0%
!! TCAAAAATAAATCTATAATA+GGG + chr6_1:100261552-100261571 Msa0902270:intron 10.0%
!! TTGAAAAATTTCTTATATTA+AGG - chr6_1:100261838-100261857 None:intergenic 10.0%
!!! AAATTTTTCAAAAAAAGTTT+TGG + chr6_1:100261847-100261866 Msa0902270:intron 10.0%
!! CTTATTATTAATTTAAGTGA+TGG + chr6_1:100261277-100261296 Msa0902270:intron 15.0%
!! TTATATGAAACCAAAAATAA+TGG + chr6_1:100261743-100261762 Msa0902270:intron 15.0%
!! AAAAGACAAATATTAAATGA+GGG - chr6_1:100261935-100261954 None:intergenic 15.0%
!! AAAAAGACAAATATTAAATG+AGG - chr6_1:100261936-100261955 None:intergenic 15.0%
!! TAATAAACAAAATGTTGTCT+TGG + chr6_1:100261354-100261373 Msa0902270:intron 20.0%
!! AAATTTCTTATATTAAGGAC+CGG - chr6_1:100261833-100261852 None:intergenic 20.0%
!!! TGGTTTTTCTTCTTATATTA+AGG + chr6_1:100262063-100262082 Msa0902270:intron 20.0%
!!! GTAGACATTATTTTCAAATT+AGG - chr6_1:100262117-100262136 None:intergenic 20.0%
!!! ACATTAAATTTTGTTTGCAT+TGG + chr6_1:100262135-100262154 Msa0902270:intron 20.0%
!!! TAGTTGGATTTTTAATAATG+AGG + chr6_1:100262695-100262714 Msa0902270:CDS 20.0%
!!! TTTGTGAGTTTTTTTTCTTT+AGG + chr6_1:100262722-100262741 Msa0902270:exon 20.0%
!! AGTTTGAATATGCATTGTTT+TGG - chr6_1:100260551-100260570 None:intergenic 25.0%
!!! TTGAAAATTTCTAGCTTTTC+TGG - chr6_1:100260721-100260740 None:intergenic 25.0%
!! TTTTCAAATCACTAGAAAAC+TGG + chr6_1:100260734-100260753 Msa0902270:CDS 25.0%
! TTTCAAATCACTAGAAAACT+GGG + chr6_1:100260735-100260754 Msa0902270:CDS 25.0%
! TGATCAGTAAATTCATCAAA+TGG - chr6_1:100260841-100260860 None:intergenic 25.0%
! ATTTGATGAATTTACTGATC+AGG + chr6_1:100260840-100260859 Msa0902270:CDS 25.0%
!! TTATTTGTATCTTTCTGGAA+GGG + chr6_1:100261080-100261099 Msa0902270:CDS 25.0%
!! TGTACATTTTATTGCAATCT+AGG + chr6_1:100261211-100261230 Msa0902270:intron 25.0%
! TATATGATCAGAGATACTAA+TGG - chr6_1:100261530-100261549 None:intergenic 25.0%
!!! AGAATACTCTGTTTTGTTTT+TGG + chr6_1:100261652-100261671 Msa0902270:intron 25.0%
!!! GAATACTCTGTTTTGTTTTT+GGG + chr6_1:100261653-100261672 Msa0902270:intron 25.0%
!! CATGAAAACTCCATTATTTT+TGG - chr6_1:100261756-100261775 None:intergenic 25.0%
! ATGTTTCACAACATTCTATA+AGG + chr6_1:100261976-100261995 Msa0902270:intron 25.0%
! TGTTTCACAACATTCTATAA+GGG + chr6_1:100261977-100261996 Msa0902270:intron 25.0%
!!! ACATTGGTTTTGATGTTTTT+TGG + chr6_1:100262043-100262062 Msa0902270:intron 25.0%
! TTTCTTCTTATATTAAGGAC+CGG + chr6_1:100262068-100262087 Msa0902270:intron 25.0%
!!! ATGTATTTTGAGTCTAATGT+TGG + chr6_1:100262859-100262878 Msa0902270:three_prime_UTR 25.0%
! TTTTGGATTTGATACTAGCT+AGG - chr6_1:100260534-100260553 None:intergenic 30.0%
TTAAGACACTTCCATTAACA+TGG + chr6_1:100260581-100260600 Msa0902270:CDS 30.0%
! TTTGGAATGTTCCATGTTAA+TGG - chr6_1:100260595-100260614 None:intergenic 30.0%
!!! AAAATTTCTAGCTTTTCTGG+TGG - chr6_1:100260718-100260737 None:intergenic 30.0%
AATTCATCAAATGGCAATGA+AGG - chr6_1:100260832-100260851 None:intergenic 30.0%
AACACAACAAAATACTCATC+AGG - chr6_1:100260898-100260917 None:intergenic 30.0%
!! TGATGAGTATTTTGTTGTGT+TGG + chr6_1:100260897-100260916 Msa0902270:CDS 30.0%
TTAATCATACTCTGATACGT+CGG - chr6_1:100260952-100260971 None:intergenic 30.0%
ACGTATCAGAGTATGATTAA+TGG + chr6_1:100260952-100260971 Msa0902270:CDS 30.0%
! AGTATGATTAATGGTCTTGA+TGG + chr6_1:100260961-100260980 Msa0902270:CDS 30.0%
AGGACTTATTTGTATCTTTC+TGG + chr6_1:100261075-100261094 Msa0902270:CDS 30.0%
CTTATTTGTATCTTTCTGGA+AGG + chr6_1:100261079-100261098 Msa0902270:CDS 30.0%
AAAACTGTTCAGTATCTCAT+TGG + chr6_1:100261123-100261142 Msa0902270:CDS 30.0%
GGGATTCTATTTAGATAGAT+AGG - chr6_1:100261590-100261609 None:intergenic 30.0%
TATGTGATCAAACACAAACA+GGG - chr6_1:100261611-100261630 None:intergenic 30.0%
ATATGTGATCAAACACAAAC+AGG - chr6_1:100261612-100261631 None:intergenic 30.0%
GTATTCTGTGATCAAATGAA+AGG - chr6_1:100261639-100261658 None:intergenic 30.0%
! AACAGTAACACATTCTGTTT+TGG - chr6_1:100261680-100261699 None:intergenic 30.0%
! AATGGAGTTTTCATGTGTTA+CGG + chr6_1:100261761-100261780 Msa0902270:intron 30.0%
ACTTAATAATATAGTCCCTC+CGG + chr6_1:100261811-100261830 Msa0902270:intron 30.0%
TGTGACAGTTGCTTATAAAT+AGG - chr6_1:100261874-100261893 None:intergenic 30.0%
ACAACAATTAATGCTGACAT+TGG + chr6_1:100262027-100262046 Msa0902270:intron 30.0%
!! AAATTTTGTTTGCATTGGAC+AGG + chr6_1:100262140-100262159 Msa0902270:intron 30.0%
TGTATACAAATCCCAAGTAT+GGG - chr6_1:100262185-100262204 None:intergenic 30.0%
! TCTTGGAGTTTTTAATCGAA+CGG + chr6_1:100262529-100262548 Msa0902270:CDS 30.0%
AGACTAGAGATTAAGAGTTA+GGG + chr6_1:100262748-100262767 Msa0902270:three_prime_UTR 30.0%
ACTAATCTTGACACTAAACA+AGG + chr6_1:100262787-100262806 Msa0902270:three_prime_UTR 30.0%
TTGTGTGGATGAAAAATAGT+TGG + chr6_1:100262919-100262938 Msa0902270:three_prime_UTR 30.0%
TGTGTGGATGAAAAATAGTT+GGG + chr6_1:100262920-100262939 Msa0902270:three_prime_UTR 30.0%
! TGAAAAATAGTTGGGACTTT+TGG + chr6_1:100262928-100262947 Msa0902270:three_prime_UTR 30.0%
TGAATTTACTGATCAGGTGA+AGG + chr6_1:100260846-100260865 Msa0902270:CDS 35.0%
CAACAAAATACTCATCAGGA+AGG - chr6_1:100260894-100260913 None:intergenic 35.0%
!! GAGTATTTTGTTGTGTTGGT+TGG + chr6_1:100260901-100260920 Msa0902270:CDS 35.0%
!! ATTAATGGTCTTGATGGTGT+TGG + chr6_1:100260967-100260986 Msa0902270:CDS 35.0%
ACTGCTGAAGAAAATAGACA+TGG + chr6_1:100261042-100261061 Msa0902270:CDS 35.0%
CTGCTGAAGAAAATAGACAT+GGG + chr6_1:100261043-100261062 Msa0902270:CDS 35.0%
TGCTGAAGAAAATAGACATG+GGG + chr6_1:100261044-100261063 Msa0902270:CDS 35.0%
! TGATGGATAGTCATAGTAGT+AGG + chr6_1:100261294-100261313 Msa0902270:intron 35.0%
!! TCATAGTAGTAGGAGTGTTT+AGG + chr6_1:100261304-100261323 Msa0902270:intron 35.0%
ATGGAAAGCAACATTGAAAG+AGG - chr6_1:100261511-100261530 None:intergenic 35.0%
ATGTGATCAAACACAAACAG+GGG - chr6_1:100261610-100261629 None:intergenic 35.0%
TTCTTATATTAAGGACCGGA+GGG - chr6_1:100262072-100262091 None:intergenic 35.0%
TTTCTTATATTAAGGACCGG+AGG - chr6_1:100261830-100261849 None:intergenic 35.0%
! AGCAACTGTCACAACTTTTA+AGG + chr6_1:100261881-100261900 Msa0902270:intron 35.0%
TTCTTATATTAAGGACCGGA+GGG + chr6_1:100262072-100262091 Msa0902270:intron 35.0%
ACAGAAAACAACCCATACTT+GGG + chr6_1:100262171-100262190 Msa0902270:CDS 35.0%
GTGTATACAAATCCCAAGTA+TGG - chr6_1:100262186-100262205 None:intergenic 35.0%
ATTTGTATCACACGGAAACA+CGG + chr6_1:100262227-100262246 Msa0902270:CDS 35.0%
TTTCTCCATCATATCACCAA+TGG - chr6_1:100262394-100262413 None:intergenic 35.0%
GGAGAAAAAAATCACAATGC+CGG + chr6_1:100262407-100262426 Msa0902270:CDS 35.0%
GAGACTAGAGATTAAGAGTT+AGG + chr6_1:100262747-100262766 Msa0902270:three_prime_UTR 35.0%
TGACACTAAACAAGGAACTT+TGG + chr6_1:100262795-100262814 Msa0902270:three_prime_UTR 35.0%
GACACTAAACAAGGAACTTT+GGG + chr6_1:100262796-100262815 Msa0902270:three_prime_UTR 35.0%
!! TTTTGAGTCTAATGTTGGAG+AGG + chr6_1:100262864-100262883 Msa0902270:three_prime_UTR 35.0%
! GATGTTGTTACTTGTTTGTG+TGG + chr6_1:100262904-100262923 Msa0902270:three_prime_UTR 35.0%
!!! ATTTTGGTGGTGGTAATAGG+TGG - chr6_1:100260642-100260661 None:intergenic 40.0%
!!! TGGATTTTGGTGGTGGTAAT+AGG - chr6_1:100260645-100260664 None:intergenic 40.0%
!!! GTTTTGGACGTTTTTAGCGA+CGG - chr6_1:100260685-100260704 None:intergenic 40.0%
TTTACCACTACTAAAGCCAG+TGG + chr6_1:100260771-100260790 Msa0902270:CDS 40.0%
CAAATGGCAATGAAGGATCA+GGG - chr6_1:100260825-100260844 None:intergenic 40.0%
TCAAATGGCAATGAAGGATC+AGG - chr6_1:100260826-100260845 None:intergenic 40.0%
!! TGTTGTGTTGGTTGGTGATA+TGG + chr6_1:100260909-100260928 Msa0902270:CDS 40.0%
! ATAGACATGGGGATTTGCTT+AGG + chr6_1:100261055-100261074 Msa0902270:CDS 40.0%
!! TCTTTCTGGAAGGGTTGATA+TGG + chr6_1:100261089-100261108 Msa0902270:CDS 40.0%
!! TAGTAGTAGGAGTGTTTAGG+TGG + chr6_1:100261307-100261326 Msa0902270:intron 40.0%
CTTCTTATATTAAGGACCGG+AGG + chr6_1:100262071-100262090 Msa0902270:intron 40.0%
GACAGAAAACAACCCATACT+TGG + chr6_1:100262170-100262189 Msa0902270:CDS 40.0%
ATACAAATGTTGCTCGCTCT+TGG - chr6_1:100262215-100262234 None:intergenic 40.0%
CGAGCAACATTTGTATCACA+CGG + chr6_1:100262219-100262238 Msa0902270:CDS 40.0%
! GTTCTCATGACGCTTTTCAT+CGG - chr6_1:100262313-100262332 None:intergenic 40.0%
! GGTCTTGTCCATCATACATA+AGG - chr6_1:100262437-100262456 None:intergenic 40.0%
CAAGATATCCGCGTAATCAT+TGG - chr6_1:100262514-100262533 None:intergenic 40.0%
CAATGATTACGCGGATATCT+TGG + chr6_1:100262512-100262531 Msa0902270:CDS 40.0%
! TGGAGTTTTTAATCGAACGG+TGG + chr6_1:100262532-100262551 Msa0902270:CDS 40.0%
TTTAATCGAACGGTGGAGAT+TGG + chr6_1:100262539-100262558 Msa0902270:CDS 40.0%
GGAGAAGCTTGAAGGATTAA+CGG + chr6_1:100262560-100262579 Msa0902270:CDS 40.0%
GAGAAGCTTGAAGGATTAAC+GGG + chr6_1:100262561-100262580 Msa0902270:CDS 40.0%
! ATTAACGGGTGAGGGAAAAA+GGG + chr6_1:100262575-100262594 Msa0902270:CDS 40.0%
TCTTGCAACCTTCTAATCCT+AGG - chr6_1:100262627-100262646 None:intergenic 40.0%
CTCGTAAGATGAAACCACAT+GGG + chr6_1:100262664-100262683 Msa0902270:CDS 40.0%
TCGTAAGATGAAACCACATG+GGG + chr6_1:100262665-100262684 Msa0902270:CDS 40.0%
AAACAAGGAACTTTGGGTAG+TGG + chr6_1:100262802-100262821 Msa0902270:three_prime_UTR 40.0%
AACAAGGAACTTTGGGTAGT+GGG + chr6_1:100262803-100262822 Msa0902270:three_prime_UTR 40.0%
GTCTAATGTTGGAGAGGATA+TGG + chr6_1:100262870-100262889 Msa0902270:three_prime_UTR 40.0%
!! CTTTTCTGGTGGCATTGAGT+GGG - chr6_1:100260707-100260726 None:intergenic 45.0%
!! TGTTCCACTGGCTTTAGTAG+TGG - chr6_1:100260778-100260797 None:intergenic 45.0%
TAAAGCCAGTGGAACAATGC+TGG + chr6_1:100260782-100260801 Msa0902270:CDS 45.0%
GGATCAGGGAGAAAATTCTG+TGG - chr6_1:100260811-100260830 None:intergenic 45.0%
!! GTTGGTTGGTGATATGGTGA+CGG + chr6_1:100260915-100260934 Msa0902270:CDS 45.0%
!! GATGGTGTTGGTGATGAGAT+TGG + chr6_1:100260979-100260998 Msa0902270:CDS 45.0%
GTTCAGTATCTCATTGGAGC+TGG + chr6_1:100261129-100261148 Msa0902270:CDS 45.0%
GTCCATTTAGTTACTCCCTC+CGG - chr6_1:100262090-100262109 None:intergenic 45.0%
!! TATGGGTTGTTTTCTGTCCC+GGG - chr6_1:100262168-100262187 None:intergenic 45.0%
!! GTATGGGTTGTTTTCTGTCC+CGG - chr6_1:100262169-100262188 None:intergenic 45.0%
! CGTCGAGAAGCTTCTAGAAA+TGG + chr6_1:100262347-100262366 Msa0902270:CDS 45.0%
! GCTAGCCATTGGTGATATGA+TGG + chr6_1:100262386-100262405 Msa0902270:CDS 45.0%
CCATCATACATAAGGTGTGC+CGG - chr6_1:100262429-100262448 None:intergenic 45.0%
! CGAAGTGCTCGAATAGCTTA+GGG - chr6_1:100262458-100262477 None:intergenic 45.0%
TAAGCTATTCGAGCACTTCG+CGG + chr6_1:100262458-100262477 Msa0902270:CDS 45.0%
ATCATTGGCCGTGTAAACTC+CGG - chr6_1:100262499-100262518 None:intergenic 45.0%
TTACACGGCCAATGATTACG+CGG + chr6_1:100262503-100262522 Msa0902270:CDS 45.0%
TGGAGATTGGAGAAGCTTGA+AGG + chr6_1:100262552-100262571 Msa0902270:CDS 45.0%
! CTTGAAGGATTAACGGGTGA+GGG + chr6_1:100262567-100262586 Msa0902270:CDS 45.0%
! GATTAACGGGTGAGGGAAAA+AGG + chr6_1:100262574-100262593 Msa0902270:CDS 45.0%
GGTTAGCACCTAGGATTAGA+AGG + chr6_1:100262616-100262635 Msa0902270:CDS 45.0%
GCTCGTAAGATGAAACCACA+TGG + chr6_1:100262663-100262682 Msa0902270:CDS 45.0%
CAACTAAACTTGACCCCATG+TGG - chr6_1:100262681-100262700 None:intergenic 45.0%
CACATGGGGTCAAGTTTAGT+TGG + chr6_1:100262679-100262698 Msa0902270:CDS 45.0%
ACAAGGAACTTTGGGTAGTG+GGG + chr6_1:100262804-100262823 Msa0902270:three_prime_UTR 45.0%
!!! GGTCGGTGGTTGATGGATTT+TGG - chr6_1:100260613-100260632 None:intergenic 50.0%
GTAATAGGTGGCGATGTGGT+CGG - chr6_1:100260630-100260649 None:intergenic 50.0%
!!! TTGGACGTTTTTAGCGACGG+TGG - chr6_1:100260682-100260701 None:intergenic 50.0%
!! TGGTGGCATTGAGTGGGTTT+TGG - chr6_1:100260701-100260720 None:intergenic 50.0%
!! GCTTTTCTGGTGGCATTGAG+TGG - chr6_1:100260708-100260727 None:intergenic 50.0%
GTATCTCATTGGAGCTGGCA+TGG + chr6_1:100261134-100261153 Msa0902270:CDS 50.0%
GACCGGAGGGAGTAACTAAA+TGG + chr6_1:100262085-100262104 Msa0902270:intron 50.0%
!! ATGGGTTGTTTTCTGTCCCG+GGG - chr6_1:100262167-100262186 None:intergenic 50.0%
! GCTTCTAGAAATGGACCCCA+CGG + chr6_1:100262356-100262375 Msa0902270:CDS 50.0%
! CTTCTAGAAATGGACCCCAC+GGG + chr6_1:100262357-100262376 Msa0902270:CDS 50.0%
CCGGCACACCTTATGTATGA+TGG + chr6_1:100262426-100262445 Msa0902270:CDS 50.0%
! GCGAAGTGCTCGAATAGCTT+AGG - chr6_1:100262459-100262478 None:intergenic 50.0%
! GCTTGAAGGATTAACGGGTG+AGG + chr6_1:100262566-100262585 Msa0902270:CDS 50.0%
!! TTGTGTGTGGGTTAGCACCT+AGG + chr6_1:100262607-100262626 Msa0902270:CDS 50.0%
! CAAGGAACTTTGGGTAGTGG+GGG + chr6_1:100262805-100262824 Msa0902270:three_prime_UTR 50.0%
GGTGGTAATAGGTGGCGATG+TGG - chr6_1:100260634-100260653 None:intergenic 55.0%
!! GTGACGGTGGATTTTGGTGG+TGG - chr6_1:100260652-100260671 None:intergenic 55.0%
!!! GTGGTGACGGTGGATTTTGG+TGG - chr6_1:100260655-100260674 None:intergenic 55.0%
!!! GTGGTGGTGACGGTGGATTT+TGG - chr6_1:100260658-100260677 None:intergenic 55.0%
! TTTTAGCGACGGTGGTGTGG+TGG - chr6_1:100260674-100260693 None:intergenic 55.0%
!!! CGTTTTTAGCGACGGTGGTG+TGG - chr6_1:100260677-100260696 None:intergenic 55.0%
AGTCCAAGCCCGTGTCCAAA+CGG - chr6_1:100261025-100261044 None:intergenic 55.0%
GTTTGCATTGGACAGGACCC+CGG + chr6_1:100262147-100262166 Msa0902270:intron 55.0%
TTTGCATTGGACAGGACCCC+GGG + chr6_1:100262148-100262167 Msa0902270:intron 55.0%
ACGGCTCGCTTAGCGAAAGA+TGG + chr6_1:100262246-100262265 Msa0902270:CDS 55.0%
GCTCGCTTAGCGAAAGATGG+CGG + chr6_1:100262249-100262268 Msa0902270:CDS 55.0%
!! ACGCTTTTCATCGGCTGCGA+TGG - chr6_1:100262304-100262323 None:intergenic 55.0%
ATGGCTAGCATTGCTCCCGT+GGG - chr6_1:100262375-100262394 None:intergenic 55.0%
AATGGCTAGCATTGCTCCCG+TGG - chr6_1:100262376-100262395 None:intergenic 55.0%
ACGGGAGCAATGCTAGCCAT+TGG + chr6_1:100262375-100262394 Msa0902270:CDS 55.0%
ACAGCGCACCGGAGTTTACA+CGG + chr6_1:100262488-100262507 Msa0902270:CDS 55.0%
! AGGGCGCAGGATTTTGTGTG+TGG + chr6_1:100262594-100262613 Msa0902270:CDS 55.0%
! GGGCGCAGGATTTTGTGTGT+GGG + chr6_1:100262595-100262614 Msa0902270:CDS 55.0%
GCGATGTGGTCGGTGGTTGA+TGG - chr6_1:100260620-100260639 None:intergenic 60.0%
ATAGGTGGCGATGTGGTCGG+TGG - chr6_1:100260627-100260646 None:intergenic 60.0%
GGCTGCCAGCATTGTTCCAC+TGG - chr6_1:100260790-100260809 None:intergenic 60.0%
GGTCCTTCGTGATCGCACGA+AGG + chr6_1:100260867-100260886 Msa0902270:CDS 60.0%
GGTCCTTCGTGCGATCACGA+AGG - chr6_1:100260873-100260892 None:intergenic 60.0%
TTGGTTCCAGTCCAAGCCCG+TGG + chr6_1:100260998-100261017 Msa0902270:CDS 60.0%
TGGTTCCAGTCCAAGCCCGT+GGG + chr6_1:100260999-100261018 Msa0902270:CDS 60.0%
CCACATATGCGCGCAAGCAC+TGG - chr6_1:100262279-100262298 None:intergenic 60.0%
CCAGTGCTTGCGCGCATATG+TGG + chr6_1:100262276-100262295 Msa0902270:CDS 60.0%
TGGCTAGCATTGCTCCCGTG+GGG - chr6_1:100262374-100262393 None:intergenic 60.0%
ATTCGAGCACTTCGCGGCTG+TGG + chr6_1:100262464-100262483 Msa0902270:CDS 60.0%
!! GGAACTTTGGGTAGTGGGGG+TGG + chr6_1:100262808-100262827 Msa0902270:three_prime_UTR 60.0%
! CGACGGTGGTGTGGTGGTGA+CGG - chr6_1:100260668-100260687 None:intergenic 65.0%
! GGGTGAGGGAAAAAGGGCGC+AGG + chr6_1:100262581-100262600 Msa0902270:CDS 65.0%
!! CGGTGGTGTGGTGGTGACGG+TGG - chr6_1:100260665-100260684 None:intergenic 70.0%
AACGGCCCACGGGCTTGGAC+TGG - chr6_1:100261007-100261026 None:intergenic 70.0%
GTCCAAGCCCGTGGGCCGTT+TGG + chr6_1:100261007-100261026 Msa0902270:CDS 70.0%
GTCCAAACGGCCCACGGGCT+TGG - chr6_1:100261012-100261031 None:intergenic 70.0%
GCCCGTGGGCCGTTTGGACA+CGG + chr6_1:100261013-100261032 Msa0902270:CDS 70.0%
CCCGTGTCCAAACGGCCCAC+GGG - chr6_1:100261017-100261036 None:intergenic 70.0%
CCCGTGGGCCGTTTGGACAC+GGG + chr6_1:100261014-100261033 Msa0902270:CDS 70.0%
GCCCGTGTCCAAACGGCCCA+CGG - chr6_1:100261018-100261037 None:intergenic 70.0%
GGGCCGTTTGGACACGGGCT+TGG + chr6_1:100261019-100261038 Msa0902270:CDS 70.0%
GCGGCTGTGGCACAGCGCAC+CGG + chr6_1:100262477-100262496 Msa0902270:CDS 75.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 100260510 100263050 100260510 ID=Msa0902270;Name=Msa0902270
chr6_1 mRNA 100260510 100263050 100260510 ID=Msa0902270-mRNA-1;Parent=Msa0902270;Name=Msa0902270-mRNA-1;_AED=0.03;_eAED=0.03;_QI=46|1|1|1|1|1|2|322|388
chr6_1 exon 100260510 100261155 100260510 ID=Msa0902270-mRNA-1:exon:17727;Parent=Msa0902270-mRNA-1
chr6_1 exon 100262162 100263050 100262162 ID=Msa0902270-mRNA-1:exon:17728;Parent=Msa0902270-mRNA-1
chr6_1 five_prime_UTR 100260510 100260555 100260510 ID=Msa0902270-mRNA-1:five_prime_utr;Parent=Msa0902270-mRNA-1
chr6_1 CDS 100260556 100261155 100260556 ID=Msa0902270-mRNA-1:cds;Parent=Msa0902270-mRNA-1
chr6_1 CDS 100262162 100262728 100262162 ID=Msa0902270-mRNA-1:cds;Parent=Msa0902270-mRNA-1
chr6_1 three_prime_UTR 100262729 100263050 100262729 ID=Msa0902270-mRNA-1:three_prime_utr;Parent=Msa0902270-mRNA-1
Gene Sequence

>Msa0902270

ATGCATATTCAAACTTGTCACTCAATTAAGACACTTCCATTAACATGGAACATTCCAAAATCCATCAACCACCGACCACATCGCCACCTATTACCACCACCAAAATCCACCGTCACCACCACACCACCGTCGCTAAAAACGTCCAAAACCCACTCAATGCCACCAGAAAAGCTAGAAATTTTCAAATCACTAGAAAACTGGGCTTCTGAGTCTGTTTTACCACTACTAAAGCCAGTGGAACAATGCTGGCAGCCACAGAATTTTCTCCCTGATCCTTCATTGCCATTTGATGAATTTACTGATCAGGTGAAGGTCCTTCGTGATCGCACGAAGGACCTTCCTGATGAGTATTTTGTTGTGTTGGTTGGTGATATGGTGACGGAAGACGCGCTTCCGACGTATCAGAGTATGATTAATGGTCTTGATGGTGTTGGTGATGAGATTGGTTCCAGTCCAAGCCCGTGGGCCGTTTGGACACGGGCTTGGACTGCTGAAGAAAATAGACATGGGGATTTGCTTAGGACTTATTTGTATCTTTCTGGAAGGGTTGATATGGAGATGATTGAGAAAACTGTTCAGTATCTCATTGGAGCTGGCATGGACCCCGGGACAGAAAACAACCCATACTTGGGATTTGTATACACATCATTCCAAGAGCGAGCAACATTTGTATCACACGGAAACACGGCTCGCTTAGCGAAAGATGGCGGCGATCCAGTGCTTGCGCGCATATGTGGTACCATCGCAGCCGATGAAAAGCGTCATGAGAACGCATACTCAAAAATCGTCGAGAAGCTTCTAGAAATGGACCCCACGGGAGCAATGCTAGCCATTGGTGATATGATGGAGAAAAAAATCACAATGCCGGCACACCTTATGTATGATGGACAAGACCCTAAGCTATTCGAGCACTTCGCGGCTGTGGCACAGCGCACCGGAGTTTACACGGCCAATGATTACGCGGATATCTTGGAGTTTTTAATCGAACGGTGGAGATTGGAGAAGCTTGAAGGATTAACGGGTGAGGGAAAAAGGGCGCAGGATTTTGTGTGTGGGTTAGCACCTAGGATTAGAAGGTTGCAAGAGAGAGCTGATGCACGTGCTCGTAAGATGAAACCACATGGGGTCAAGTTTAGTTGGATTTTTAATAATGAGGTTGTTTTGTGA
Protein sequence

>Msa0902270

MHIQTCHSIKTLPLTWNIPKSINHRPHRHLLPPPKSTVTTTPPSLKTSKTHSMPPEKLEIFKSLENWASESVLPLLKPVEQCWQPQNFLPDPSLPFDEFTDQVKVLRDRTKDLPDEYFVVLVGDMVTEDALPTYQSMINGLDGVGDEIGSSPSPWAVWTRAWTAEENRHGDLLRTYLYLSGRVDMEMIEKTVQYLIGAGMDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKDGGDPVLARICGTIAADEKRHENAYSKIVEKLLEMDPTGAMLAIGDMMEKKITMPAHLMYDGQDPKLFEHFAAVAQRTGVYTANDYADILEFLIERWRLEKLEGLTGEGKRAQDFVCGLAPRIRRLQERADARARKMKPHGVKFSWIFNNEVVL*