AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0575950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575950 MtrunA17_Chr8g0393241 98.875 800 8 1 1 800 1 799 0.0 1619
Msa0575950 MtrunA17_Chr6g0451081 45.980 796 384 12 13 797 13 773 0.0 705
Msa0575950 MtrunA17_Chr6g0461871 45.558 788 394 11 18 797 22 782 0.0 674
Msa0575950 MtrunA17_Chr3g0128831 43.125 800 420 10 12 797 13 791 0.0 656
Msa0575950 MtrunA17_Chr5g0430731 42.018 783 387 14 23 797 28 751 0.0 600
Msa0575950 MtrunA17_Chr8g0387661 39.949 786 436 11 22 796 28 788 0.0 572
Msa0575950 MtrunA17_Chr5g0413721 37.416 890 424 21 10 797 10 868 0.0 548
Msa0575950 MtrunA17_Chr2g0297291 37.690 788 447 11 13 797 40 786 4.26e-172 515
Msa0575950 MtrunA17_Chr4g0052401 38.383 779 430 14 23 797 52 784 8.54e-169 507
Msa0575950 MtrunA17_Chr4g0055471 34.875 800 477 12 8 800 53 815 4.54e-163 493
Msa0575950 MtrunA17_Chr8g0365001 35.088 798 471 10 8 796 77 836 9.41e-163 493
Msa0575950 MtrunA17_Chr7g0268981 34.919 799 441 15 1 796 47 769 1.26e-156 475
Msa0575950 MtrunA17_Chr2g0279131 34.204 804 472 12 7 796 85 845 1.09e-144 446
Msa0575950 MtrunA17_Chr5g0429991 33.086 807 477 13 7 796 85 845 1.16e-136 426
Msa0575950 MtrunA17_Chr8g0380281 31.790 799 494 13 10 800 27 782 3.71e-136 422
Msa0575950 MtrunA17_Chr5g0430571 39.182 587 277 14 214 797 23 532 1.10e-131 402
Msa0575950 MtrunA17_Chr8g0375411 36.783 628 363 9 7 633 15 609 1.10e-130 405
Msa0575950 MtrunA17_Chr8g0344561 34.951 618 344 10 186 796 2 568 3.95e-123 382
Msa0575950 MtrunA17_Chr8g0344591 37.333 450 254 7 186 630 2 428 4.80e-96 307
Msa0575950 MtrunA17_Chr8g0365031 34.468 470 286 9 213 673 44 500 6.78e-94 304
Msa0575950 MtrunA17_Chr2g0297311 41.748 412 209 6 29 435 58 443 3.95e-91 294
Msa0575950 MtrunA17_Chr5g0413751 55.682 176 78 0 437 612 133 308 1.39e-65 221
Msa0575950 MtrunA17_Chr5g0413751 43.158 95 50 2 80 170 47 141 1.89e-11 66.2
Msa0575950 MtrunA17_Chr2g0297321 35.054 368 202 7 439 797 5 344 6.02e-64 218
Msa0575950 MtrunA17_Chr5g0430551 46.250 160 82 2 21 177 11 169 1.52e-36 135
Msa0575950 MtrunA17_Chr8g0365041 40.331 181 106 2 2 181 44 223 4.83e-35 133
Msa0575950 MtrunA17_Chr8g0344551 38.562 153 90 1 20 172 3 151 9.74e-32 120
Msa0575950 MtrunA17_Chr8g0344581 38.562 153 90 1 20 172 3 151 1.55e-31 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575950 AT1G70300.1 44.279 804 405 14 10 800 9 782 0.0 684
Msa0575950 AT2G40540.1 43.911 813 402 13 8 797 10 791 0.0 674
Msa0575950 AT2G40540.5 43.911 813 402 13 8 797 10 791 0.0 674
Msa0575950 AT2G40540.4 43.911 813 402 13 8 797 10 791 0.0 674
Msa0575950 AT2G40540.2 43.911 813 402 13 8 797 10 791 0.0 674
Msa0575950 AT2G40540.3 43.911 813 402 13 8 797 10 791 0.0 674
Msa0575950 AT5G14880.1 43.875 800 400 12 13 797 13 778 0.0 654
Msa0575950 AT3G02050.1 45.625 800 379 13 18 797 23 786 0.0 649
Msa0575950 AT4G23640.1 39.272 797 445 12 11 796 3 771 0.0 563
Msa0575950 AT2G30070.1 39.203 778 374 10 23 797 28 709 0.0 561
Msa0575950 AT1G31120.1 36.469 776 439 10 29 797 65 793 1.42e-166 502
Msa0575950 AT4G13420.1 36.143 783 441 11 17 796 55 781 2.39e-166 501
Msa0575950 AT1G60160.1 35.472 795 458 11 9 796 77 823 4.81e-163 494
Msa0575950 AT2G35060.4 36.305 774 438 14 29 797 66 789 3.31e-160 485
Msa0575950 AT2G35060.1 36.305 774 438 14 29 797 66 789 3.31e-160 485
Msa0575950 AT2G35060.3 36.305 774 438 14 29 797 66 789 3.31e-160 485
Msa0575950 AT2G35060.2 36.258 775 438 15 29 797 66 790 1.89e-158 481
Msa0575950 AT4G19960.2 37.339 774 453 10 25 797 62 804 5.23e-154 470
Msa0575950 AT4G19960.3 36.876 781 473 8 25 797 62 830 7.92e-153 468
Msa0575950 AT2G30070.2 37.135 684 334 9 114 797 2 589 1.52e-152 459
Msa0575950 AT4G19960.1 36.582 790 453 11 25 797 62 820 8.82e-150 459
Msa0575950 AT4G33530.1 34.355 783 476 11 18 796 103 851 8.16e-139 432
Msa0575950 AT5G09400.1 33.082 795 490 9 10 796 94 854 6.06e-135 422
Msa0575950 AT4G19960.4 37.265 585 355 6 214 797 10 583 1.35e-123 384
Msa0575950 AT4G33530.2 34.317 644 386 10 156 796 14 623 7.21e-114 359

Find 169 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGTCATTTCAAATTAT+TGG 0.097572 4_1:+79946133 Msa0575950:CDS
TAATACCTTGATTGATTTCT+TGG 0.133225 4_1:-79947850 None:intergenic
CAAATTATTGGAATAGTTTA+TGG 0.171289 4_1:+79946145 Msa0575950:CDS
TCACTATTTGATATTGTTCT+TGG 0.177064 4_1:+79946594 Msa0575950:CDS
ATGAGATACCTTTCACTTTC+GGG 0.185728 4_1:-79949244 None:intergenic
CCCTGCGGCACACATAAAAT+TGG 0.218769 4_1:+79947503 Msa0575950:CDS
TGCTAGTAGTAGTCCTAAAA+TGG 0.222069 4_1:+79949636 Msa0575950:CDS
CCCACCTGCTGCGCATAAAA+TGG 0.247149 4_1:-79947693 None:intergenic
CTCAATGGCCCTTCGTGCTT+TGG 0.254731 4_1:-79946557 None:intergenic
TTGCTTGATCTATCCGCTTC+TGG 0.258921 4_1:+79947979 Msa0575950:CDS
GATATTGTGATAATAATATA+AGG 0.265580 4_1:+79949320 Msa0575950:CDS
ATATTGTTCTTGGCTCTCTT+TGG 0.271962 4_1:+79946604 Msa0575950:CDS
AAAAGCCAAACAGCAATAAT+TGG 0.277240 4_1:-79947539 None:intergenic
GCATGCTTTCTGTTATGTTT+TGG 0.280359 4_1:+79948881 Msa0575950:CDS
CACTAATCATTGCTTTGTAT+TGG 0.284123 4_1:+79948837 Msa0575950:CDS
TTGGTTCCTGTATGACAATT+GGG 0.284904 4_1:+79946623 Msa0575950:CDS
TAGACAAGAAGTTATTTCTT+TGG 0.288483 4_1:-79950025 None:intergenic
GTCATGCTTTCATGGCATTA+TGG 0.288725 4_1:+79948995 Msa0575950:CDS
TTTGGTTCCTGTATGACAAT+TGG 0.290612 4_1:+79946622 Msa0575950:CDS
GTCAAAAGATGGAGATTATT+AGG 0.292066 4_1:+79947665 Msa0575950:CDS
ACTAATCATTGCTTTGTATT+GGG 0.300084 4_1:+79948838 Msa0575950:CDS
ATTTCAAGGGTAGCTGGAAT+TGG 0.306668 4_1:+79949097 Msa0575950:CDS
CATTGTCCCGAATACATATA+AGG 0.308500 4_1:-79946185 None:intergenic
ACGAATTTGAACAGGTATAG+TGG 0.308769 4_1:-79947629 None:intergenic
ATGTGACTGTCCCAAGAAAT+AGG 0.320049 4_1:-79949716 None:intergenic
TTGGTATCTAGTTGTCCTTT+TGG 0.329914 4_1:+79948958 Msa0575950:CDS
ACTGCTCCCTTATATGTATT+CGG 0.332081 4_1:+79946178 Msa0575950:CDS
CGCAGGGCTGCAGCACGAAC+AGG 0.333248 4_1:-79947487 None:intergenic
TCCTGCTGCTCATGACTCTA+AGG 0.336816 4_1:+79945968 Msa0575950:CDS
GAGTGGTAGTGCCTATTTCT+TGG 0.340865 4_1:+79949705 Msa0575950:CDS
AGTATTGTCCCTACTTTCTT+CGG 0.348739 4_1:+79948199 Msa0575950:CDS
TATGAGATACCTTTCACTTT+CGG 0.349566 4_1:-79949245 None:intergenic
GTCTCTCATTAATTAATTCT+AGG 0.349884 4_1:+79949918 Msa0575950:three_prime_UTR
GGGTCAGAGGCAATGTTTGC+AGG 0.358591 4_1:+79947813 Msa0575950:intron
CTTGGGACAGTCACATTTAG+TGG 0.358649 4_1:+79949723 Msa0575950:CDS
TATCGTTGCATTGTGAGATA+TGG 0.370461 4_1:+79949298 Msa0575950:CDS
TTGCTCCAATTATTGCTGTT+TGG 0.374670 4_1:+79947534 Msa0575950:CDS
ATCCCAGAAGAAAATATTAT+AGG 0.374775 4_1:-79947582 None:intergenic
AGCTGGCTGTTATAGTTGCT+TGG 0.383170 4_1:-79948232 None:intergenic
GTGCTCAAAGCCGATGATAA+AGG 0.386986 4_1:+79946307 Msa0575950:CDS
CCAGTGTTATCAGCTTCATA+TGG 0.390257 4_1:+79946761 Msa0575950:intron
CGATGACAGTCATGCTTTCA+TGG 0.391628 4_1:+79948987 Msa0575950:CDS
CAATACTATGAATACATGTT+TGG 0.391978 4_1:-79948183 None:intergenic
GAGAAAATCATTGGTGCTAT+AGG 0.397323 4_1:+79949361 Msa0575950:CDS
TTTCGTTTCTTTGAAAAGAA+TGG 0.402669 4_1:+79949790 Msa0575950:CDS
TCTGCGTGTGTTATATTGAT+TGG 0.403406 4_1:+79947464 Msa0575950:CDS
TTCAGAGACATGGTGAAGAT+TGG 0.404906 4_1:+79948398 Msa0575950:CDS
TGACATTGAATCAATGGTTT+CGG 0.408757 4_1:+79949411 Msa0575950:CDS
TATCAGAACAGAACAGGAAT+GGG 0.426721 4_1:-79945918 None:intergenic
CTGCTCCCTTATATGTATTC+GGG 0.427071 4_1:+79946179 Msa0575950:CDS
GATGAGTGACATTGAATCAA+TGG 0.427788 4_1:+79949405 Msa0575950:CDS
TTTCAAGGGTAGCTGGAATT+GGG 0.431440 4_1:+79949098 Msa0575950:CDS
GGTGATATGCTATGCGGGTC+AGG 0.433632 4_1:+79948000 Msa0575950:CDS
TGCTAGACATTGGTTAATGA+TGG 0.434347 4_1:-79948258 None:intergenic
ATAGATGTCAGTCCAGGCCT+TGG 0.434506 4_1:+79949073 Msa0575950:CDS
TGCAATTTCACAAGGGAGCT+TGG 0.436192 4_1:+79948939 Msa0575950:CDS
AATGTCACTCATCTGTTCAA+TGG 0.442300 4_1:-79949395 None:intergenic
TTTACATCTCCGATGTCAAC+TGG 0.442324 4_1:+79948339 Msa0575950:CDS
TCTCAAGAGATTCTTAATCA+TGG 0.442855 4_1:+79949765 Msa0575950:CDS
AGTGGTAGTGCCTATTTCTT+GGG 0.442863 4_1:+79949706 Msa0575950:CDS
TCACTTTCCTCAATTACTGC+TGG 0.445037 4_1:-79949547 None:intergenic
GTCCAGGCCTTGGGATTTCA+AGG 0.446718 4_1:+79949083 Msa0575950:CDS
ATCTGTGAGGAAGGAGCTGT+TGG 0.455362 4_1:+79949663 Msa0575950:CDS
TATTCCTCACGAATTTGAAC+AGG 0.458746 4_1:-79947637 None:intergenic
CATGTTTCATCATTCATCAA+AGG 0.462722 4_1:-79949487 None:intergenic
ATGTTTCATCATTCATCAAA+GGG 0.464890 4_1:-79949486 None:intergenic
GTCCTCACTCCAGCACTCTC+AGG 0.466444 4_1:+79946652 Msa0575950:CDS
ATAAAATGTGAAAAGAAAGC+TGG 0.467438 4_1:-79949151 None:intergenic
TCCCTACTTTCTTCGGCTGT+CGG 0.468003 4_1:+79948206 Msa0575950:CDS
AGGTATCTCATAGGAAGGAT+TGG 0.477155 4_1:+79949256 Msa0575950:CDS
TATGATTATCAGAACAGAAC+AGG 0.480109 4_1:-79945924 None:intergenic
ACCCTTGAAATCCCAAGGCC+TGG 0.485489 4_1:-79949085 None:intergenic
GAGGCAATGTTTGCAGGCCT+AGG 0.489080 4_1:+79947819 Msa0575950:CDS
TGACATCGGAGATGTAAATC+TGG 0.489377 4_1:-79948334 None:intergenic
GAAAGTGAAAGGTATCTCAT+AGG 0.491062 4_1:+79949247 Msa0575950:CDS
ACAGGACTTGTGTTTCTCAA+TGG 0.493275 4_1:-79946572 None:intergenic
CTTGGGATTTCAAGGGTAGC+TGG 0.493441 4_1:+79949091 Msa0575950:CDS
ATATTGTGATAATAATATAA+GGG 0.493754 4_1:+79949321 Msa0575950:CDS
CGTACCCTCTCCTTTATCAT+CGG 0.494667 4_1:-79946317 None:intergenic
TAGATGTCAGTCCAGGCCTT+GGG 0.495201 4_1:+79949074 Msa0575950:CDS
CACTTTCCTCAATTACTGCT+GGG 0.497373 4_1:-79949546 None:intergenic
CGAATTTGAACAGGTATAGT+GGG 0.498758 4_1:-79947628 None:intergenic
CATGCTGCTCTTGTAGAAGT+TGG 0.499402 4_1:+79949844 Msa0575950:CDS
CCTGCGGCACACATAAAATT+GGG 0.499524 4_1:+79947504 Msa0575950:CDS
GGTATCTCATAGGAAGGATT+GGG 0.500620 4_1:+79949257 Msa0575950:CDS
ATCAACACTTGATGAAATGC+AGG 0.501496 4_1:-79949184 None:intergenic
TCAGAGATCCTTGTCAGACT+TGG 0.504330 4_1:+79946787 Msa0575950:CDS
ATTGTCCCGAATACATATAA+GGG 0.505493 4_1:-79946184 None:intergenic
ACCTTAGAGTCATGAGCAGC+AGG 0.505650 4_1:-79945969 None:intergenic
GGAAAGTGATCATACATTGT+TGG 0.507113 4_1:+79949561 Msa0575950:CDS
TAAAATGTGAAAAGAAAGCT+GGG 0.508339 4_1:-79949150 None:intergenic
ATAACACACGCAGAGGGAAC+AGG 0.516134 4_1:-79947455 None:intergenic
TGCGGCCCCAATTGTCATAC+AGG 0.516547 4_1:-79946629 None:intergenic
GGAATATTGATGTCAAAAGA+TGG 0.518956 4_1:+79947654 Msa0575950:CDS
AACAATTTAGTGCTAGACAT+TGG 0.524283 4_1:-79948268 None:intergenic
ACTATGAATACATGTTTGGA+TGG 0.524692 4_1:-79948179 None:intergenic
ATCGGCTTTGAGCACTATGA+TGG 0.526999 4_1:-79946299 None:intergenic
TTCAGTCTAGCTGTGACGGT+TGG 0.528040 4_1:+79948374 Msa0575950:CDS
TGATAGATAGATAGAATCAA+AGG 0.530754 4_1:+79949980 Msa0575950:three_prime_UTR
GCTAGTAGTAGTCCTAAAAT+GGG 0.535308 4_1:+79949637 Msa0575950:CDS
TTTCATCGTCACAACATACT+CGG 0.535954 4_1:+79947055 Msa0575950:CDS
TTGTGTAGAAATGCAAAAGT+TGG 0.539250 4_1:+79946451 Msa0575950:CDS
ATTTCATCAAGTGTTGATCA+TGG 0.541920 4_1:+79949189 Msa0575950:CDS
GATTAGCCCAGCAGTAATTG+AGG 0.542723 4_1:+79949540 Msa0575950:CDS
AAAACAAGGTGTCAACTGAA+TGG 0.543678 4_1:+79949047 Msa0575950:CDS
ATTTACACAGACATAGTAGC+AGG 0.543683 4_1:+79949124 Msa0575950:CDS
CTGTCATCGACACAACCAAA+AGG 0.544019 4_1:-79948973 None:intergenic
GAACAATATCAAATAGTGAC+AGG 0.547114 4_1:-79946590 None:intergenic
TCAGTATTAATCTTAGAAGA+AGG 0.549138 4_1:-79946532 None:intergenic
CTTCTGGTGATATGCTATGC+GGG 0.549853 4_1:+79947995 Msa0575950:CDS
TCCGACAGCCGAAGAAAGTA+GGG 0.550295 4_1:-79948207 None:intergenic
TTCCGACAGCCGAAGAAAGT+AGG 0.550320 4_1:-79948208 None:intergenic
CTGGACCAACTTACGTGGTA+CGG 0.552439 4_1:-79948077 None:intergenic
TGGCTAATAGATGTCAGTCC+AGG 0.553837 4_1:+79949067 Msa0575950:CDS
CTTTCGGGAACATGAGGCAC+CGG 0.556705 4_1:-79949229 None:intergenic
CTAATTGATGCAAGGGAGAG+TGG 0.560075 4_1:+79949688 Msa0575950:CDS
TACTGATTCCAAAGCACGAA+GGG 0.560127 4_1:+79946549 Msa0575950:CDS
GAGTTCATAAACAACTTCCT+CGG 0.565549 4_1:-79946227 None:intergenic
TGGTTCCTGTATGACAATTG+GGG 0.565614 4_1:+79946624 Msa0575950:CDS
TTGGTTAATGATGGAGAAGC+TGG 0.565734 4_1:-79948249 None:intergenic
CTTTCACTTTCGGGAACATG+AGG 0.566786 4_1:-79949235 None:intergenic
CATTAATTAATTCTAGGAGG+AGG 0.573002 4_1:+79949924 Msa0575950:three_prime_UTR
TTATCAGAACAGAACAGGAA+TGG 0.575430 4_1:-79945919 None:intergenic
CTCATGTTCCCGAAAGTGAA+AGG 0.575661 4_1:+79949236 Msa0575950:CDS
TGTATACCACCTGAGAGTGC+TGG 0.576733 4_1:-79946661 None:intergenic
TGGTTCTATCACAAGGGAGT+AGG 0.578415 4_1:-79946474 None:intergenic
ATAGTGGGAAATTCATCAGA+TGG 0.578894 4_1:+79949451 Msa0575950:CDS
GTTGGAACTAATTGATGCAA+GGG 0.584636 4_1:+79949681 Msa0575950:CDS
GTGAAAGGTATCTCATAGGA+AGG 0.585522 4_1:+79949251 Msa0575950:CDS
TGAGTTTGATTTGCAAAACA+AGG 0.593927 4_1:+79949033 Msa0575950:CDS
TTCATCGTCACAACATACTC+GGG 0.594104 4_1:+79947056 Msa0575950:CDS
AATGGGAGAATCTGTGAGGA+AGG 0.595349 4_1:+79949654 Msa0575950:CDS
TCTCATTAATTAATTCTAGG+AGG 0.596868 4_1:+79949921 Msa0575950:three_prime_UTR
CAGCTACCCTTGAAATCCCA+AGG 0.598668 4_1:-79949090 None:intergenic
GGTGTCATTCAAGTCAATGC+CGG 0.599286 4_1:+79949210 Msa0575950:CDS
TGTTGGAACTAATTGATGCA+AGG 0.600387 4_1:+79949680 Msa0575950:CDS
GTGTATGTTGCAATTTCACA+AGG 0.607535 4_1:+79948931 Msa0575950:CDS
GCAGGTTTGGCTATAATATG+TGG 0.608232 4_1:+79948788 Msa0575950:intron
TGATAATAATATAAGGGACA+CGG 0.608313 4_1:+79949327 Msa0575950:CDS
TCACATTTAGTGGTTCGTGA+TGG 0.611078 4_1:+79949733 Msa0575950:CDS
GATAATAATATAAGGGACAC+GGG 0.611099 4_1:+79949328 Msa0575950:CDS
TCATCAGTGGTTCTATCACA+AGG 0.619268 4_1:-79946481 None:intergenic
GCATAGCATATCACCAGAAG+CGG 0.619528 4_1:-79947992 None:intergenic
AGCGCAAGATCTTGAATCCG+AGG 0.620300 4_1:+79946210 Msa0575950:CDS
TCCAGGCCTTGGGATTTCAA+GGG 0.627014 4_1:+79949084 Msa0575950:CDS
CTGTTCGTGCTGCAGCCCTG+CGG 0.631273 4_1:+79947488 Msa0575950:CDS
TTGTGATAGAACCACTGATG+AGG 0.635745 4_1:+79946483 Msa0575950:CDS
ATCAATATAACACACGCAGA+GGG 0.639548 4_1:-79947461 None:intergenic
GACGGTTGGTTTCAGAGACA+TGG 0.641262 4_1:+79948388 Msa0575950:CDS
ACTTCTACAAGAGCAGCATG+AGG 0.642823 4_1:-79949841 None:intergenic
CATCAATAACCAGTTGACAT+CGG 0.643303 4_1:-79948348 None:intergenic
CTTCTACAAGAGCAGCATGA+GGG 0.644345 4_1:-79949840 None:intergenic
CACCTGAGAGTGCTGGAGTG+AGG 0.647966 4_1:-79946654 None:intergenic
CTCACTCCAGCACTCTCAGG+TGG 0.651315 4_1:+79946655 Msa0575950:CDS
CAAAGCCGATGATAAAGGAG+AGG 0.656962 4_1:+79946312 Msa0575950:CDS
GCTTCTGGTGATATGCTATG+CGG 0.658421 4_1:+79947994 Msa0575950:CDS
ATCAGAACAGAACAGGAATG+GGG 0.661510 4_1:-79945917 None:intergenic
TAACTGTGCCAAGTCTGACA+AGG 0.665484 4_1:-79946795 None:intergenic
AATCAATATAACACACGCAG+AGG 0.681941 4_1:-79947462 None:intergenic
AAAGCCGATGATAAAGGAGA+GGG 0.682612 4_1:+79946313 Msa0575950:CDS
TTAGGACTACTACTAGCAGG+AGG 0.682982 4_1:-79949631 None:intergenic
TATACCTGTTCAAATTCGTG+AGG 0.684600 4_1:+79947633 Msa0575950:CDS
GTTCTGATAATCATATCACA+TGG 0.685690 4_1:+79945932 Msa0575950:exon
CATCAGTGGTTCTATCACAA+GGG 0.691255 4_1:-79946480 None:intergenic
GAATTTGAACAGGTATAGTG+GGG 0.699929 4_1:-79947627 None:intergenic
CTAAAATGGGAGAATCTGTG+AGG 0.712297 4_1:+79949650 Msa0575950:CDS
ATACTGATTCCAAAGCACGA+AGG 0.716209 4_1:+79946548 Msa0575950:CDS
GAGTGCTGGAGTGAGGACTG+CGG 0.718425 4_1:-79946647 None:intergenic
TGTATGTTGCAATTTCACAA+GGG 0.739609 4_1:+79948932 Msa0575950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTCTGAAAAAATAATA+GGG - chr4_1:79946095-79946114 None:intergenic 10.0%
!!! TTTTATTCTGAAAAAATAAT+AGG - chr4_1:79946096-79946115 None:intergenic 10.0%
!! ATATTGTGATAATAATATAA+GGG + chr4_1:79949321-79949340 Msa0575950:CDS 10.0%
!!! TATTTTTTCAGAATAAAAGA+TGG + chr4_1:79946098-79946117 Msa0575950:intron 15.0%
!! GTAGCTAAAATAATAAATAT+AGG - chr4_1:79946853-79946872 None:intergenic 15.0%
!! AATTCTAATTAACAATCATT+TGG - chr4_1:79947755-79947774 None:intergenic 15.0%
!!! ATTGTTAATTAGAATTGTTT+TGG + chr4_1:79947759-79947778 Msa0575950:intron 15.0%
!!! CGATATATTTTATAATCTTT+TGG - chr4_1:79949283-79949302 None:intergenic 15.0%
!! GATATTGTGATAATAATATA+AGG + chr4_1:79949320-79949339 Msa0575950:CDS 15.0%
!! CAAATTATTGGAATAGTTTA+TGG + chr4_1:79946145-79946164 Msa0575950:CDS 20.0%
!! TTCAATGTAGTGAATATAAT+TGG - chr4_1:79946393-79946412 None:intergenic 20.0%
!!! ATTTATTATTTTAGCTACCA+AGG + chr4_1:79946855-79946874 Msa0575950:intron 20.0%
!! AAGAAAAGCAGTAAATATAT+TGG - chr4_1:79946922-79946941 None:intergenic 20.0%
!!! TTTTAGTTTTTGAAATCTGA+TGG + chr4_1:79947310-79947329 Msa0575950:intron 20.0%
!!! TTCTAGTTTAATGCAAAAAT+GGG - chr4_1:79948508-79948527 None:intergenic 20.0%
!!! TTTCTAGTTTAATGCAAAAA+TGG - chr4_1:79948509-79948528 None:intergenic 20.0%
!!! AAAAGTTGGTAAATTAAACA+GGG + chr4_1:79948570-79948589 Msa0575950:intron 20.0%
!! CTTGTGATTTGATTAAATTA+AGG + chr4_1:79948668-79948687 Msa0575950:intron 20.0%
!! TGATTTGATTAAATTAAGGA+TGG + chr4_1:79948672-79948691 Msa0575950:intron 20.0%
!! TTAAATTAAGGATGGTTATA+TGG + chr4_1:79948680-79948699 Msa0575950:intron 20.0%
!! TTAATTAAGTTCACAATTTG+TGG + chr4_1:79948766-79948785 Msa0575950:intron 20.0%
!! CTCTTGTCATTTCAAATTAT+TGG + chr4_1:79946133-79946152 Msa0575950:CDS 25.0%
!! CTACATTGAAAATATTTTCC+AGG + chr4_1:79946403-79946422 Msa0575950:intron 25.0%
! TCAGTATTAATCTTAGAAGA+AGG - chr4_1:79946535-79946554 None:intergenic 25.0%
! TCACTATTTGATATTGTTCT+TGG + chr4_1:79946594-79946613 Msa0575950:CDS 25.0%
!! GCAGAAAGTATATAAATCTT+AGG - chr4_1:79946734-79946753 None:intergenic 25.0%
!! TTTTTGTAGAACATTTGAGA+CGG - chr4_1:79947085-79947104 None:intergenic 25.0%
!! AGCCTATAATATTTTCTTCT+GGG + chr4_1:79947580-79947599 Msa0575950:CDS 25.0%
! ATCCCAGAAGAAAATATTAT+AGG - chr4_1:79947585-79947604 None:intergenic 25.0%
!!! GTTAATTAGAATTGTTTTGG+AGG + chr4_1:79947762-79947781 Msa0575950:intron 25.0%
!! TGATTGATTTCTTGGAAAAA+TGG - chr4_1:79947845-79947864 None:intergenic 25.0%
!! TTTTTCCAAGAAATCAATCA+AGG + chr4_1:79947845-79947864 Msa0575950:CDS 25.0%
!! TAATACCTTGATTGATTTCT+TGG - chr4_1:79947853-79947872 None:intergenic 25.0%
! AATCAATTTACTAGAACTAC+AGG - chr4_1:79947891-79947910 None:intergenic 25.0%
!!! AAAGGCATCTTAGAAATAAA+GGG - chr4_1:79948112-79948131 None:intergenic 25.0%
!!! AAAAGGCATCTTAGAAATAA+AGG - chr4_1:79948113-79948132 None:intergenic 25.0%
! CAATACTATGAATACATGTT+TGG - chr4_1:79948186-79948205 None:intergenic 25.0%
! ACCAAGAGAAATGAAAAAAA+TGG - chr4_1:79948440-79948459 None:intergenic 25.0%
! TTTGAGTCCTTAATTTATGA+AGG + chr4_1:79948530-79948549 Msa0575950:intron 25.0%
! TTGAGTCCTTAATTTATGAA+GGG + chr4_1:79948531-79948550 Msa0575950:intron 25.0%
! GTCTTAAGAAAATGAAAAGT+TGG + chr4_1:79948556-79948575 Msa0575950:intron 25.0%
!!! GAAAAGTTGGTAAATTAAAC+AGG + chr4_1:79948569-79948588 Msa0575950:intron 25.0%
! ACTAATCATTGCTTTGTATT+GGG + chr4_1:79948838-79948857 Msa0575950:CDS 25.0%
! TAAAATGTGAAAAGAAAGCT+GGG - chr4_1:79949153-79949172 None:intergenic 25.0%
! ATAAAATGTGAAAAGAAAGC+TGG - chr4_1:79949154-79949173 None:intergenic 25.0%
! TGATAATAATATAAGGGACA+CGG + chr4_1:79949327-79949346 Msa0575950:CDS 25.0%
! AGAAGAAAAAATGATGATAG+TGG + chr4_1:79949435-79949454 Msa0575950:CDS 25.0%
! GAAGAAAAAATGATGATAGT+GGG + chr4_1:79949436-79949455 Msa0575950:CDS 25.0%
! ATGTTTCATCATTCATCAAA+GGG - chr4_1:79949489-79949508 None:intergenic 25.0%
! GCAACAAAAGAAAAAAAGTA+CGG + chr4_1:79949599-79949618 Msa0575950:CDS 25.0%
!! TTTCGTTTCTTTGAAAAGAA+TGG + chr4_1:79949790-79949809 Msa0575950:CDS 25.0%
! GTCTCTCATTAATTAATTCT+AGG + chr4_1:79949918-79949937 Msa0575950:three_prime_UTR 25.0%
! TCTCATTAATTAATTCTAGG+AGG + chr4_1:79949921-79949940 Msa0575950:three_prime_UTR 25.0%
! TGATAGATAGATAGAATCAA+AGG + chr4_1:79949980-79949999 Msa0575950:three_prime_UTR 25.0%
TATGATTATCAGAACAGAAC+AGG - chr4_1:79945927-79945946 None:intergenic 30.0%
GTTCTGATAATCATATCACA+TGG + chr4_1:79945932-79945951 Msa0575950:exon 30.0%
ATTGTCCCGAATACATATAA+GGG - chr4_1:79946187-79946206 None:intergenic 30.0%
!!! AACTCTTCTCCTTCATTTTT+TGG + chr4_1:79946245-79946264 Msa0575950:CDS 30.0%
TGTTAGAGTCCAAAAAATGA+AGG - chr4_1:79946257-79946276 None:intergenic 30.0%
! CATTGAAAATATTTTCCAGG+TGG + chr4_1:79946406-79946425 Msa0575950:intron 30.0%
TATAAAGCAAAAATACCACC+TGG - chr4_1:79946424-79946443 None:intergenic 30.0%
TTGTGTAGAAATGCAAAAGT+TGG + chr4_1:79946451-79946470 Msa0575950:CDS 30.0%
GAACAATATCAAATAGTGAC+AGG - chr4_1:79946593-79946612 None:intergenic 30.0%
CTTCAGAAAAGAAAATGTCA+TGG - chr4_1:79946976-79946995 None:intergenic 30.0%
! CATGACATTTTCTTTTCTGA+AGG + chr4_1:79946974-79946993 Msa0575950:intron 30.0%
CACAATTATCCTCGATAAAT+TGG - chr4_1:79947238-79947257 None:intergenic 30.0%
AAAAGCCAAACAGCAATAAT+TGG - chr4_1:79947542-79947561 None:intergenic 30.0%
! GAGCCTATAATATTTTCTTC+TGG + chr4_1:79947579-79947598 Msa0575950:CDS 30.0%
GGAATATTGATGTCAAAAGA+TGG + chr4_1:79947654-79947673 Msa0575950:CDS 30.0%
GTCAAAAGATGGAGATTATT+AGG + chr4_1:79947665-79947684 Msa0575950:CDS 30.0%
TTTAGCAAAGCGACTAAAAA+AGG - chr4_1:79948130-79948149 None:intergenic 30.0%
ACTATGAATACATGTTTGGA+TGG - chr4_1:79948182-79948201 None:intergenic 30.0%
AACAATTTAGTGCTAGACAT+TGG - chr4_1:79948271-79948290 None:intergenic 30.0%
!!! GCCATTTTTTTCATTTCTCT+TGG + chr4_1:79948436-79948455 Msa0575950:intron 30.0%
AGACTTCCCTTCATAAATTA+AGG - chr4_1:79948540-79948559 None:intergenic 30.0%
TTAATATATACTACCAGCAC+AGG + chr4_1:79948616-79948635 Msa0575950:intron 30.0%
AAATCACAAGTACAGTAGTT+CGG - chr4_1:79948658-79948677 None:intergenic 30.0%
CACTAATCATTGCTTTGTAT+TGG + chr4_1:79948837-79948856 Msa0575950:CDS 30.0%
AAAAATATGCAACCTCAACA+AGG - chr4_1:79948907-79948926 None:intergenic 30.0%
! TGTTGAGGTTGCATATTTTT+CGG + chr4_1:79948907-79948926 Msa0575950:CDS 30.0%
TGTATGTTGCAATTTCACAA+GGG + chr4_1:79948932-79948951 Msa0575950:CDS 30.0%
!! TGAGTTTGATTTGCAAAACA+AGG + chr4_1:79949033-79949052 Msa0575950:CDS 30.0%
!! ATTTCATCAAGTGTTGATCA+TGG + chr4_1:79949189-79949208 Msa0575950:CDS 30.0%
TATGAGATACCTTTCACTTT+CGG - chr4_1:79949248-79949267 None:intergenic 30.0%
GATAATAATATAAGGGACAC+GGG + chr4_1:79949328-79949347 Msa0575950:CDS 30.0%
!! GATTTTGAGGAGAAAATCAT+TGG + chr4_1:79949352-79949371 Msa0575950:CDS 30.0%
TGACATTGAATCAATGGTTT+CGG + chr4_1:79949411-79949430 Msa0575950:CDS 30.0%
CATGTTTCATCATTCATCAA+AGG - chr4_1:79949490-79949509 None:intergenic 30.0%
TCTCAAGAGATTCTTAATCA+TGG + chr4_1:79949765-79949784 Msa0575950:CDS 30.0%
! CATTAATTAATTCTAGGAGG+AGG + chr4_1:79949924-79949943 Msa0575950:three_prime_UTR 30.0%
TATCAGAACAGAACAGGAAT+GGG - chr4_1:79945921-79945940 None:intergenic 35.0%
TTATCAGAACAGAACAGGAA+TGG - chr4_1:79945922-79945941 None:intergenic 35.0%
AAGAGTGAGACTGAATATGA+TGG - chr4_1:79946003-79946022 None:intergenic 35.0%
!! AATGATCTTTCTAGTTCCCT+AGG + chr4_1:79946043-79946062 Msa0575950:intron 35.0%
ACTGCTCCCTTATATGTATT+CGG + chr4_1:79946178-79946197 Msa0575950:CDS 35.0%
CATTGTCCCGAATACATATA+AGG - chr4_1:79946188-79946207 None:intergenic 35.0%
GAGTTCATAAACAACTTCCT+CGG - chr4_1:79946230-79946249 None:intergenic 35.0%
! ATATTGTTCTTGGCTCTCTT+TGG + chr4_1:79946604-79946623 Msa0575950:CDS 35.0%
! TTTGGTTCCTGTATGACAAT+TGG + chr4_1:79946622-79946641 Msa0575950:CDS 35.0%
TTGGTTCCTGTATGACAATT+GGG + chr4_1:79946623-79946642 Msa0575950:CDS 35.0%
TTTCATCGTCACAACATACT+CGG + chr4_1:79947055-79947074 Msa0575950:CDS 35.0%
!! ACCAATTGACTCGACATAAA+GGG - chr4_1:79947154-79947173 None:intergenic 35.0%
!! AATTGGTCGATTTGTGTGTT+TGG + chr4_1:79947167-79947186 Msa0575950:intron 35.0%
ATTGGATGCATAGTGCTAAT+TGG - chr4_1:79947220-79947239 None:intergenic 35.0%
ACTATGCATCCAATTTATCG+AGG + chr4_1:79947226-79947245 Msa0575950:intron 35.0%
! GTATTTAATTTAACCCGCCA+TGG - chr4_1:79947264-79947283 None:intergenic 35.0%
!! CATGTAGTTTGCTTCTAGTT+TGG + chr4_1:79947283-79947302 Msa0575950:intron 35.0%
CTGGAAATCAATTGAGCAAA+AGG - chr4_1:79947430-79947449 None:intergenic 35.0%
ATCAATATAACACACGCAGA+GGG - chr4_1:79947464-79947483 None:intergenic 35.0%
AATCAATATAACACACGCAG+AGG - chr4_1:79947465-79947484 None:intergenic 35.0%
TCTGCGTGTGTTATATTGAT+TGG + chr4_1:79947464-79947483 Msa0575950:CDS 35.0%
! TTGCTCCAATTATTGCTGTT+TGG + chr4_1:79947534-79947553 Msa0575950:CDS 35.0%
GAATTTGAACAGGTATAGTG+GGG - chr4_1:79947630-79947649 None:intergenic 35.0%
CGAATTTGAACAGGTATAGT+GGG - chr4_1:79947631-79947650 None:intergenic 35.0%
ACGAATTTGAACAGGTATAG+TGG - chr4_1:79947632-79947651 None:intergenic 35.0%
TATACCTGTTCAAATTCGTG+AGG + chr4_1:79947633-79947652 Msa0575950:CDS 35.0%
TATTCCTCACGAATTTGAAC+AGG - chr4_1:79947640-79947659 None:intergenic 35.0%
! TCATGCTCTACAAATTTTGC+AGG + chr4_1:79947792-79947811 Msa0575950:intron 35.0%
! CATGCTCTACAAATTTTGCA+GGG + chr4_1:79947793-79947812 Msa0575950:intron 35.0%
ATTTCTTGGAAAAATGGCCT+AGG - chr4_1:79947839-79947858 None:intergenic 35.0%
TTTACTAGAACTACAGGACA+AGG - chr4_1:79947885-79947904 None:intergenic 35.0%
AGTATTGTCCCTACTTTCTT+CGG + chr4_1:79948199-79948218 Msa0575950:CDS 35.0%
! TGCTAGACATTGGTTAATGA+TGG - chr4_1:79948261-79948280 None:intergenic 35.0%
! GTGTGAATGACTTTTACTCT+GGG - chr4_1:79948296-79948315 None:intergenic 35.0%
! TGTGTGAATGACTTTTACTC+TGG - chr4_1:79948297-79948316 None:intergenic 35.0%
CACACATCAAAAACGATACA+TGG + chr4_1:79948311-79948330 Msa0575950:CDS 35.0%
CATCAATAACCAGTTGACAT+CGG - chr4_1:79948351-79948370 None:intergenic 35.0%
TTAAGTTCACAATTTGTGGC+AGG + chr4_1:79948770-79948789 Msa0575950:intron 35.0%
! GCATGCTTTCTGTTATGTTT+TGG + chr4_1:79948881-79948900 Msa0575950:CDS 35.0%
GTGTATGTTGCAATTTCACA+AGG + chr4_1:79948931-79948950 Msa0575950:CDS 35.0%
! TTGGTATCTAGTTGTCCTTT+TGG + chr4_1:79948958-79948977 Msa0575950:CDS 35.0%
! AAAACAAGGTGTCAACTGAA+TGG + chr4_1:79949047-79949066 Msa0575950:CDS 35.0%
ATTTACACAGACATAGTAGC+AGG + chr4_1:79949124-79949143 Msa0575950:CDS 35.0%
ATCAACACTTGATGAAATGC+AGG - chr4_1:79949187-79949206 None:intergenic 35.0%
ATGAGATACCTTTCACTTTC+GGG - chr4_1:79949247-79949266 None:intergenic 35.0%
!! GAAAGTGAAAGGTATCTCAT+AGG + chr4_1:79949247-79949266 Msa0575950:CDS 35.0%
TATCGTTGCATTGTGAGATA+TGG + chr4_1:79949298-79949317 Msa0575950:CDS 35.0%
!! GAGAAAATCATTGGTGCTAT+AGG + chr4_1:79949361-79949380 Msa0575950:CDS 35.0%
AATGTCACTCATCTGTTCAA+TGG - chr4_1:79949398-79949417 None:intergenic 35.0%
GATGAGTGACATTGAATCAA+TGG + chr4_1:79949405-79949424 Msa0575950:CDS 35.0%
! ATAGTGGGAAATTCATCAGA+TGG + chr4_1:79949451-79949470 Msa0575950:CDS 35.0%
GGAAAGTGATCATACATTGT+TGG + chr4_1:79949561-79949580 Msa0575950:CDS 35.0%
!!! ATTTTAGGACTACTACTAGC+AGG - chr4_1:79949637-79949656 None:intergenic 35.0%
TGCTAGTAGTAGTCCTAAAA+TGG + chr4_1:79949636-79949655 Msa0575950:CDS 35.0%
GCTAGTAGTAGTCCTAAAAT+GGG + chr4_1:79949637-79949656 Msa0575950:CDS 35.0%
TGTTGGAACTAATTGATGCA+AGG + chr4_1:79949680-79949699 Msa0575950:CDS 35.0%
GTTGGAACTAATTGATGCAA+GGG + chr4_1:79949681-79949700 Msa0575950:CDS 35.0%
ATCAGAACAGAACAGGAATG+GGG - chr4_1:79945920-79945939 None:intergenic 40.0%
! GTGAGACTGAATATGATGGA+TGG - chr4_1:79945999-79946018 None:intergenic 40.0%
GGGAGACAGAAATCATGAAA+AGG - chr4_1:79946075-79946094 None:intergenic 40.0%
CTGCTCCCTTATATGTATTC+GGG + chr4_1:79946179-79946198 Msa0575950:CDS 40.0%
AAAGCCGATGATAAAGGAGA+GGG + chr4_1:79946313-79946332 Msa0575950:CDS 40.0%
! CATCAGTGGTTCTATCACAA+GGG - chr4_1:79946483-79946502 None:intergenic 40.0%
! TCATCAGTGGTTCTATCACA+AGG - chr4_1:79946484-79946503 None:intergenic 40.0%
TTGTGATAGAACCACTGATG+AGG + chr4_1:79946483-79946502 Msa0575950:CDS 40.0%
!! ATACTGATTCCAAAGCACGA+AGG + chr4_1:79946548-79946567 Msa0575950:CDS 40.0%
!! TACTGATTCCAAAGCACGAA+GGG + chr4_1:79946549-79946568 Msa0575950:CDS 40.0%
ACAGGACTTGTGTTTCTCAA+TGG - chr4_1:79946575-79946594 None:intergenic 40.0%
TGGTTCCTGTATGACAATTG+GGG + chr4_1:79946624-79946643 Msa0575950:CDS 40.0%
AGCTGATAACACTGGTAAAC+AGG - chr4_1:79946756-79946775 None:intergenic 40.0%
CCATATGAAGCTGATAACAC+TGG - chr4_1:79946764-79946783 None:intergenic 40.0%
CCAGTGTTATCAGCTTCATA+TGG + chr4_1:79946761-79946780 Msa0575950:intron 40.0%
TTAGCTACCAAGGATACTAC+TGG + chr4_1:79946865-79946884 Msa0575950:intron 40.0%
! ACCAAGGATACTACTGGTTA+AGG + chr4_1:79946871-79946890 Msa0575950:intron 40.0%
TCCTTAACCAGTAGTATCCT+TGG - chr4_1:79946875-79946894 None:intergenic 40.0%
GTATGTTGTGACGATGAAAC+TGG - chr4_1:79947054-79947073 None:intergenic 40.0%
TTCATCGTCACAACATACTC+GGG + chr4_1:79947056-79947075 Msa0575950:CDS 40.0%
TCCCTTTATGTCGAGTCAAT+TGG + chr4_1:79947150-79947169 Msa0575950:intron 40.0%
!! GACCAATTGACTCGACATAA+AGG - chr4_1:79947155-79947174 None:intergenic 40.0%
CGAGGATAATTGTGTATCCA+TGG + chr4_1:79947244-79947263 Msa0575950:intron 40.0%
GGATAATTGTGTATCCATGG+CGG + chr4_1:79947247-79947266 Msa0575950:intron 40.0%
GATAATTGTGTATCCATGGC+GGG + chr4_1:79947248-79947267 Msa0575950:intron 40.0%
CAATTGAGCAAAAGGTCAAG+AGG - chr4_1:79947422-79947441 None:intergenic 40.0%
!!! GCTGTTTGGCTTTTGTTTGT+TGG + chr4_1:79947548-79947567 Msa0575950:CDS 40.0%
!! TTGGCTTTTGTTTGTTGGTG+TGG + chr4_1:79947553-79947572 Msa0575950:CDS 40.0%
!! CTTTTGTTTGTTGGTGTGGT+GGG + chr4_1:79947557-79947576 Msa0575950:CDS 40.0%
! TACAAATTTTGCAGGGTCAG+AGG + chr4_1:79947800-79947819 Msa0575950:intron 40.0%
TCTTAGAAATAAAGGGTGGC+AGG - chr4_1:79948105-79948124 None:intergenic 40.0%
GGCATCTTAGAAATAAAGGG+TGG - chr4_1:79948109-79948128 None:intergenic 40.0%
! TTGGTTAATGATGGAGAAGC+TGG - chr4_1:79948252-79948271 None:intergenic 40.0%
TGACATCGGAGATGTAAATC+TGG - chr4_1:79948337-79948356 None:intergenic 40.0%
TTTACATCTCCGATGTCAAC+TGG + chr4_1:79948339-79948358 Msa0575950:CDS 40.0%
! GATTTTCAGTCTAGCTGTGA+CGG + chr4_1:79948370-79948389 Msa0575950:CDS 40.0%
TTCAGAGACATGGTGAAGAT+TGG + chr4_1:79948398-79948417 Msa0575950:CDS 40.0%
GAACGATCATGCTAACTGAA+TGG - chr4_1:79948716-79948735 None:intergenic 40.0%
TTCACAATTTGTGGCAGGTT+TGG + chr4_1:79948775-79948794 Msa0575950:intron 40.0%
GCAGGTTTGGCTATAATATG+TGG + chr4_1:79948788-79948807 Msa0575950:intron 40.0%
!!! GTTATGTTTTGGCCTTGTTG+AGG + chr4_1:79948892-79948911 Msa0575950:CDS 40.0%
GTCATGCTTTCATGGCATTA+TGG + chr4_1:79948995-79949014 Msa0575950:CDS 40.0%
ATTTCAAGGGTAGCTGGAAT+TGG + chr4_1:79949097-79949116 Msa0575950:CDS 40.0%
TTTCAAGGGTAGCTGGAATT+GGG + chr4_1:79949098-79949117 Msa0575950:CDS 40.0%
!! GTGAAAGGTATCTCATAGGA+AGG + chr4_1:79949251-79949270 Msa0575950:CDS 40.0%
AGGTATCTCATAGGAAGGAT+TGG + chr4_1:79949256-79949275 Msa0575950:CDS 40.0%
GGTATCTCATAGGAAGGATT+GGG + chr4_1:79949257-79949276 Msa0575950:CDS 40.0%
CACTTTCCTCAATTACTGCT+GGG - chr4_1:79949549-79949568 None:intergenic 40.0%
TCACTTTCCTCAATTACTGC+TGG - chr4_1:79949550-79949569 None:intergenic 40.0%
! CTCACAGATTCTCCCATTTT+AGG - chr4_1:79949652-79949671 None:intergenic 40.0%
CTAAAATGGGAGAATCTGTG+AGG + chr4_1:79949650-79949669 Msa0575950:CDS 40.0%
! AGTGGTAGTGCCTATTTCTT+GGG + chr4_1:79949706-79949725 Msa0575950:CDS 40.0%
ATGTGACTGTCCCAAGAAAT+AGG - chr4_1:79949719-79949738 None:intergenic 40.0%
TCACATTTAGTGGTTCGTGA+TGG + chr4_1:79949733-79949752 Msa0575950:CDS 40.0%
! CATGAAAAGGAGCTAGCCTA+GGG - chr4_1:79946062-79946081 None:intergenic 45.0%
! TCATGAAAAGGAGCTAGCCT+AGG - chr4_1:79946063-79946082 None:intergenic 45.0%
!! ATCGGCTTTGAGCACTATGA+TGG - chr4_1:79946302-79946321 None:intergenic 45.0%
GTGCTCAAAGCCGATGATAA+AGG + chr4_1:79946307-79946326 Msa0575950:CDS 45.0%
CAAAGCCGATGATAAAGGAG+AGG + chr4_1:79946312-79946331 Msa0575950:CDS 45.0%
CGTACCCTCTCCTTTATCAT+CGG - chr4_1:79946320-79946339 None:intergenic 45.0%
! TGGTTCTATCACAAGGGAGT+AGG - chr4_1:79946477-79946496 None:intergenic 45.0%
! AAAAAGCACGACCTCATCAG+TGG - chr4_1:79946497-79946516 None:intergenic 45.0%
!! TGATGAGGTCGTGCTTTTTG+AGG + chr4_1:79946498-79946517 Msa0575950:CDS 45.0%
TCAGAGATCCTTGTCAGACT+TGG + chr4_1:79946787-79946806 Msa0575950:CDS 45.0%
TAACTGTGCCAAGTCTGACA+AGG - chr4_1:79946798-79946817 None:intergenic 45.0%
TATGCTGTGATCATGCTGCA+TGG + chr4_1:79947393-79947412 Msa0575950:intron 45.0%
! CCAATTTTATGTGTGCCGCA+GGG - chr4_1:79947506-79947525 None:intergenic 45.0%
CCTGCGGCACACATAAAATT+GGG + chr4_1:79947504-79947523 Msa0575950:CDS 45.0%
!! GCTTTTGTTTGTTGGTGTGG+TGG + chr4_1:79947556-79947575 Msa0575950:CDS 45.0%
TTGCTTGATCTATCCGCTTC+TGG + chr4_1:79947979-79947998 Msa0575950:CDS 45.0%
GCATAGCATATCACCAGAAG+CGG - chr4_1:79947995-79948014 None:intergenic 45.0%
GCTTCTGGTGATATGCTATG+CGG + chr4_1:79947994-79948013 Msa0575950:CDS 45.0%
CTTCTGGTGATATGCTATGC+GGG + chr4_1:79947995-79948014 Msa0575950:CDS 45.0%
TCAATCCGTACCACGTAAGT+TGG + chr4_1:79948072-79948091 Msa0575950:intron 45.0%
CGCTTTGCTAAAGTGATTGC+TGG + chr4_1:79948138-79948157 Msa0575950:intron 45.0%
AGCTGGCTGTTATAGTTGCT+TGG - chr4_1:79948235-79948254 None:intergenic 45.0%
TGCAATTTCACAAGGGAGCT+TGG + chr4_1:79948939-79948958 Msa0575950:CDS 45.0%
CTGTCATCGACACAACCAAA+AGG - chr4_1:79948976-79948995 None:intergenic 45.0%
CGATGACAGTCATGCTTTCA+TGG + chr4_1:79948987-79949006 Msa0575950:CDS 45.0%
TGGCTAATAGATGTCAGTCC+AGG + chr4_1:79949067-79949086 Msa0575950:CDS 45.0%
!! GGTGTCATTCAAGTCAATGC+CGG + chr4_1:79949210-79949229 Msa0575950:CDS 45.0%
CTTTCACTTTCGGGAACATG+AGG - chr4_1:79949238-79949257 None:intergenic 45.0%
CTCATGTTCCCGAAAGTGAA+AGG + chr4_1:79949236-79949255 Msa0575950:CDS 45.0%
! GATTAGCCCAGCAGTAATTG+AGG + chr4_1:79949540-79949559 Msa0575950:CDS 45.0%
TTAGGACTACTACTAGCAGG+AGG - chr4_1:79949634-79949653 None:intergenic 45.0%
AATGGGAGAATCTGTGAGGA+AGG + chr4_1:79949654-79949673 Msa0575950:CDS 45.0%
CTAATTGATGCAAGGGAGAG+TGG + chr4_1:79949688-79949707 Msa0575950:CDS 45.0%
! GAGTGGTAGTGCCTATTTCT+TGG + chr4_1:79949705-79949724 Msa0575950:CDS 45.0%
CTTGGGACAGTCACATTTAG+TGG + chr4_1:79949723-79949742 Msa0575950:CDS 45.0%
!! TGAGGGATTTTGAGAGCAAC+TGG - chr4_1:79949826-79949845 None:intergenic 45.0%
CTTCTACAAGAGCAGCATGA+GGG - chr4_1:79949843-79949862 None:intergenic 45.0%
ACTTCTACAAGAGCAGCATG+AGG - chr4_1:79949844-79949863 None:intergenic 45.0%
CATGCTGCTCTTGTAGAAGT+TGG + chr4_1:79949844-79949863 Msa0575950:CDS 45.0%
!! AAATGAAAATATAATTAAAT+GGG - chr4_1:79946692-79946711 None:intergenic 5.0%
!! AAAATGAAAATATAATTAAA+TGG - chr4_1:79946693-79946712 None:intergenic 5.0%
TCCTGCTGCTCATGACTCTA+AGG + chr4_1:79945968-79945987 Msa0575950:CDS 50.0%
ACCTTAGAGTCATGAGCAGC+AGG - chr4_1:79945972-79945991 None:intergenic 50.0%
AGCGCAAGATCTTGAATCCG+AGG + chr4_1:79946210-79946229 Msa0575950:CDS 50.0%
! TGTATACCACCTGAGAGTGC+TGG - chr4_1:79946664-79946683 None:intergenic 50.0%
ATAACACACGCAGAGGGAAC+AGG - chr4_1:79947458-79947477 None:intergenic 50.0%
CCCTGCGGCACACATAAAAT+TGG + chr4_1:79947503-79947522 Msa0575950:CDS 50.0%
! CCCAATTTTATGTGTGCCGC+AGG - chr4_1:79947507-79947526 None:intergenic 50.0%
! CCATTTTATGCGCAGCAGGT+GGG + chr4_1:79947693-79947712 Msa0575950:intron 50.0%
CTGGACCAACTTACGTGGTA+CGG - chr4_1:79948080-79948099 None:intergenic 50.0%
AAATAAAGGGTGGCAGGCAC+TGG - chr4_1:79948099-79948118 None:intergenic 50.0%
! TGCTGGTGATGACATACTGC+AGG + chr4_1:79948155-79948174 Msa0575950:intron 50.0%
TCCCTACTTTCTTCGGCTGT+CGG + chr4_1:79948206-79948225 Msa0575950:CDS 50.0%
TCCGACAGCCGAAGAAAGTA+GGG - chr4_1:79948210-79948229 None:intergenic 50.0%
TTCCGACAGCCGAAGAAAGT+AGG - chr4_1:79948211-79948230 None:intergenic 50.0%
TTCAGTCTAGCTGTGACGGT+TGG + chr4_1:79948374-79948393 Msa0575950:CDS 50.0%
!! GACGGTTGGTTTCAGAGACA+TGG + chr4_1:79948388-79948407 Msa0575950:CDS 50.0%
ATAGATGTCAGTCCAGGCCT+TGG + chr4_1:79949073-79949092 Msa0575950:CDS 50.0%
TAGATGTCAGTCCAGGCCTT+GGG + chr4_1:79949074-79949093 Msa0575950:CDS 50.0%
TCCAGGCCTTGGGATTTCAA+GGG + chr4_1:79949084-79949103 Msa0575950:CDS 50.0%
CAGCTACCCTTGAAATCCCA+AGG - chr4_1:79949093-79949112 None:intergenic 50.0%
CTTGGGATTTCAAGGGTAGC+TGG + chr4_1:79949091-79949110 Msa0575950:CDS 50.0%
! AAGGGACACGGGTGATTTTG+AGG + chr4_1:79949339-79949358 Msa0575950:CDS 50.0%
ATCTGTGAGGAAGGAGCTGT+TGG + chr4_1:79949663-79949682 Msa0575950:CDS 50.0%
!! GGGATTTTGAGAGCAACTGG+AGG - chr4_1:79949823-79949842 None:intergenic 50.0%
CTCAATGGCCCTTCGTGCTT+TGG - chr4_1:79946560-79946579 None:intergenic 55.0%
TGCGGCCCCAATTGTCATAC+AGG - chr4_1:79946632-79946651 None:intergenic 55.0%
GCAGAGGGAACAGGTACATC+TGG - chr4_1:79947449-79947468 None:intergenic 55.0%
!! AGCGCCATTTTATGCGCAGC+AGG + chr4_1:79947689-79947708 Msa0575950:CDS 55.0%
!! GCCATTTTATGCGCAGCAGG+TGG + chr4_1:79947692-79947711 Msa0575950:intron 55.0%
CCCACCTGCTGCGCATAAAA+TGG - chr4_1:79947696-79947715 None:intergenic 55.0%
GGGTCAGAGGCAATGTTTGC+AGG + chr4_1:79947813-79947832 Msa0575950:intron 55.0%
GAGGCAATGTTTGCAGGCCT+AGG + chr4_1:79947819-79947838 Msa0575950:CDS 55.0%
GGTGATATGCTATGCGGGTC+AGG + chr4_1:79948000-79948019 Msa0575950:CDS 55.0%
!! AGGCACTGGACCAACTTACG+TGG - chr4_1:79948085-79948104 None:intergenic 55.0%
TAACTGCACTGTGCCTGTGC+TGG - chr4_1:79948632-79948651 None:intergenic 55.0%
GTCCAGGCCTTGGGATTTCA+AGG + chr4_1:79949083-79949102 Msa0575950:CDS 55.0%
ACCCTTGAAATCCCAAGGCC+TGG - chr4_1:79949088-79949107 None:intergenic 55.0%
CTTTCGGGAACATGAGGCAC+CGG - chr4_1:79949232-79949251 None:intergenic 55.0%
!! GAGTGCTGGAGTGAGGACTG+CGG - chr4_1:79946650-79946669 None:intergenic 60.0%
GTCCTCACTCCAGCACTCTC+AGG + chr4_1:79946652-79946671 Msa0575950:CDS 60.0%
!! CACCTGAGAGTGCTGGAGTG+AGG - chr4_1:79946657-79946676 None:intergenic 60.0%
CTCACTCCAGCACTCTCAGG+TGG + chr4_1:79946655-79946674 Msa0575950:CDS 60.0%
CTGTTCGTGCTGCAGCCCTG+CGG + chr4_1:79947488-79947507 Msa0575950:CDS 65.0%
CGCAGGGCTGCAGCACGAAC+AGG - chr4_1:79947490-79947509 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 79945917 79950043 79945917 ID=Msa0575950;Name=Msa0575950
chr4_1 mRNA 79945917 79950043 79945917 ID=Msa0575950-mRNA-1;Parent=Msa0575950;Name=Msa0575950-mRNA-1;_AED=0.06;_eAED=0.06;_QI=34|0.88|0.9|1|0.88|1|10|158|800
chr4_1 exon 79945917 79945989 79945917 ID=Msa0575950-mRNA-1:exon:28537;Parent=Msa0575950-mRNA-1
chr4_1 exon 79946109 79946334 79946109 ID=Msa0575950-mRNA-1:exon:28538;Parent=Msa0575950-mRNA-1
chr4_1 exon 79946425 79946676 79946425 ID=Msa0575950-mRNA-1:exon:28539;Parent=Msa0575950-mRNA-1
chr4_1 exon 79946765 79946818 79946765 ID=Msa0575950-mRNA-1:exon:28540;Parent=Msa0575950-mRNA-1
chr4_1 exon 79947055 79947105 79947055 ID=Msa0575950-mRNA-1:exon:28541;Parent=Msa0575950-mRNA-1
chr4_1 exon 79947450 79947710 79947450 ID=Msa0575950-mRNA-1:exon:28542;Parent=Msa0575950-mRNA-1
chr4_1 exon 79947814 79947866 79947814 ID=Msa0575950-mRNA-1:exon:28543;Parent=Msa0575950-mRNA-1
chr4_1 exon 79947968 79948085 79947968 ID=Msa0575950-mRNA-1:exon:28544;Parent=Msa0575950-mRNA-1
chr4_1 exon 79948177 79948431 79948177 ID=Msa0575950-mRNA-1:exon:28545;Parent=Msa0575950-mRNA-1
chr4_1 exon 79948792 79950043 79948792 ID=Msa0575950-mRNA-1:exon:28546;Parent=Msa0575950-mRNA-1
chr4_1 five_prime_UTR 79945917 79945950 79945917 ID=Msa0575950-mRNA-1:five_prime_utr;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79945951 79945989 79945951 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79946109 79946334 79946109 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79946425 79946676 79946425 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79946765 79946818 79946765 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79947055 79947105 79947055 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79947450 79947710 79947450 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79947814 79947866 79947814 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79947968 79948085 79947968 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79948177 79948431 79948177 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 CDS 79948792 79949885 79948792 ID=Msa0575950-mRNA-1:cds;Parent=Msa0575950-mRNA-1
chr4_1 three_prime_UTR 79949886 79950043 79949886 ID=Msa0575950-mRNA-1:three_prime_utr;Parent=Msa0575950-mRNA-1
Gene Sequence

>Msa0575950

ATGGCTTCTCAACTGCATCCTGCTGCTCATGACTCTAAGAATAAAAGATGGAATCAAACAATTCTCTTGTCATTTCAAATTATTGGAATAGTTTATGGTCAACTTAGCACTGCTCCCTTATATGTATTCGGGACAATGCAAGCGCAAGATCTTGAATCCGAGGAAGTTGTTTATGAACTCTTCTCCTTCATTTTTTGGACTCTAACAACTATTTCTTTGATCAAGTATGCCATCATAGTGCTCAAAGCCGATGATAAAGGAGAGGGTGGTATTTTTGCTTTATACTCACTATTGTGTAGAAATGCAAAAGTTGGCCTACTCCCTTGTGATAGAACCACTGATGAGGTCGTGCTTTTTGAGGAGACAACAACTCCTTCTTCTAAGATTAATACTGATTCCAAAGCACGAAGGGCCATTGAGAAACACAAGTCCTGTCACTATTTGATATTGTTCTTGGCTCTCTTTGGTTCCTGTATGACAATTGGGGCCGCAGTCCTCACTCCAGCACTCTCAGGTGTGTTATCAGCTTCATATGGTGTTCAGAGATCCTTGTCAGACTTGGCACAGTTATTTTCATCGTCACAACATACTCGGGATTCCGTCTCAAATGTTCTACAAAAATATGTACCTGTTCCCTCTGCGTGTGTTATATTGATTGGCCTGTTCGTGCTGCAGCCCTGCGGCACACATAAAATTGGGTTCATGTTTGCTCCAATTATTGCTGTTTGGCTTTTGTTTGTTGGTGTGGTGGGAGCCTATAATATTTTCTTCTGGGATGTCAAAATTATCTATAAAATATCCCCACTATACCTGTTCAAATTCGTGAGGAATATTGATGTCAAAAGATGGAGATTATTAGGAAGCGCCATTTTATGCGCAGCAGGGTCAGAGGCAATGTTTGCAGGCCTAGGCCATTTTTCCAAGAAATCAATCAAGATTACATTTATTTGCTTGATCTATCCGCTTCTGGTGATATGCTATGCGGGTCAGGCTGCATATATCTCTAAAAACTTGAACACTCGAGATTTTAATCATCTTAGTCAATCCGTACCACGTCCATCCAAACATGTATTCATAGTATTGTCCCTACTTTCTTCGGCTGTCGGAAGCCAAGCAACTATAACAGCCAGCTTCTCCATCATTAACCAATGTCTAGCACTAAATTGTTTTCCCAGAGTAAAAGTCATTCACACATCAAAAACGATACATGGCCAGATTTACATCTCCGATGTCAACTGGTTATTGATGATTTTCAGTCTAGCTGTGACGGTTGGTTTCAGAGACATGGTGAAGATTGGCAACGCAACAAGTTTGGCTATAATATGTGGAATGCTTGTGACAACTAGTCTCATGTCACTAATCATTGCTTTGTATTGGGAGAAGAATTTGATGATATCTGCATGCTTTCTGTTATGTTTTGGCCTTGTTGAGGTTGCATATTTTTCGGCGTGTATGTTGCAATTTCACAAGGGAGCTTGGTATCTAGTTGTCCTTTTGGTTGTGTCGATGACAGTCATGCTTTCATGGCATTATGGAACAGTGAAGAAGTATGAGTTTGATTTGCAAAACAAGGTGTCAACTGAATGGCTAATAGATGTCAGTCCAGGCCTTGGGATTTCAAGGGTAGCTGGAATTGGGTTCATTTACACAGACATAGTAGCAGGAATCCCAGCTTTCTTTTCACATTTTATTACTAATCTTCCTGCATTTCATCAAGTGTTGATCATGGTGTCATTCAAGTCAATGCCGGTGCCTCATGTTCCCGAAAGTGAAAGGTATCTCATAGGAAGGATTGGGCCAAAAGATTATAAAATATATCGTTGCATTGTGAGATATGGATATTGTGATAATAATATAAGGGACACGGGTGATTTTGAGGAGAAAATCATTGGTGCTATAGGAGAATTCATTTCCATTGAACAGATGAGTGACATTGAATCAATGGTTTCGGCAGAAGAAAAAATGATGATAGTGGGAAATTCATCAGATGGAAATGCATTAGTCCCTTTGATGAATGATGAAACATGCAATGAAGTTAGCATTACTAATGAAGCTCAGATTAGCCCAGCAGTAATTGAGGAAAGTGATCATACATTGTTGGCGAGTAGTAGCTCTTGCAACAAAAGAAAAAAAGTACGGTTCATGTTGCCTCCTGCTAGTAGTAGTCCTAAAATGGGAGAATCTGTGAGGAAGGAGCTGTTGGAACTAATTGATGCAAGGGAGAGTGGTAGTGCCTATTTCTTGGGACAGTCACATTTAGTGGTTCGTGATGGCTCAAACTATCTCAAGAGATTCTTAATCATGGTTTTTCGTTTCTTTGAAAAGAATGGTCGTGAACCTCCAGTTGCTCTCAAAATCCCTCATGCTGCTCTTGTAGAAGTTGGTATGATATGTACTATATAA
Protein sequence

>Msa0575950

MASQLHPAAHDSKNKRWNQTILLSFQIIGIVYGQLSTAPLYVFGTMQAQDLESEEVVYELFSFIFWTLTTISLIKYAIIVLKADDKGEGGIFALYSLLCRNAKVGLLPCDRTTDEVVLFEETTTPSSKINTDSKARRAIEKHKSCHYLILFLALFGSCMTIGAAVLTPALSGVLSASYGVQRSLSDLAQLFSSSQHTRDSVSNVLQKYVPVPSACVILIGLFVLQPCGTHKIGFMFAPIIAVWLLFVGVVGAYNIFFWDVKIIYKISPLYLFKFVRNIDVKRWRLLGSAILCAAGSEAMFAGLGHFSKKSIKITFICLIYPLLVICYAGQAAYISKNLNTRDFNHLSQSVPRPSKHVFIVLSLLSSAVGSQATITASFSIINQCLALNCFPRVKVIHTSKTIHGQIYISDVNWLLMIFSLAVTVGFRDMVKIGNATSLAIICGMLVTTSLMSLIIALYWEKNLMISACFLLCFGLVEVAYFSACMLQFHKGAWYLVVLLVVSMTVMLSWHYGTVKKYEFDLQNKVSTEWLIDVSPGLGISRVAGIGFIYTDIVAGIPAFFSHFITNLPAFHQVLIMVSFKSMPVPHVPESERYLIGRIGPKDYKIYRCIVRYGYCDNNIRDTGDFEEKIIGAIGEFISIEQMSDIESMVSAEEKMMIVGNSSDGNALVPLMNDETCNEVSITNEAQISPAVIEESDHTLLASSSSCNKRKKVRFMLPPASSSPKMGESVRKELLELIDARESGSAYFLGQSHLVVRDGSNYLKRFLIMVFRFFEKNGREPPVALKIPHAALVEVGMICTI*