AlfalfaGEDB | Msa0395970

M. sativa cultivar zhongmu-4 / Msa0395970

KEGG: K09191
GO: GO:0000182 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006355 GO:0008097 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019843 GO:0022613 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0042254 GO:0042273 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071840 GO:0080084 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00096.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0395970	QJS95126.1	93.651	378	9	2	1	377	1	364	0.0	714

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0395970	sp\|Q84MZ4\|TF3A_ARATH	51.807	415	133	4	2	364	3	402	3.11e-142	413

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0395970	A0A6M4W3T1	93.651	378	9	2	1	377	1	364	0.0	714

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TFs/TRs:

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Gene ID	Type	Classification
Msa0395970	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa0395970	0.816127	6.489923e-52	-8.615850e-47
Msa0011170	Msa0395970	0.833477	5.213384e-56	-8.615850e-47
Msa0012040	Msa0395970	0.813691	2.251363e-51	-8.615850e-47
Msa0012080	Msa0395970	0.800604	1.333506e-48	-8.615850e-47
Msa0017510	Msa0395970	0.812556	3.994484e-51	-8.615850e-47
Msa0025730	Msa0395970	0.807208	5.655918e-50	-8.615850e-47
Msa0037700	Msa0395970	0.804361	2.241920e-49	-8.615850e-47
Msa0045920	Msa0395970	0.800006	1.764203e-48	-8.615850e-47
Msa0054440	Msa0395970	0.805896	1.069831e-49	-8.615850e-47
Msa0054480	Msa0395970	0.835242	1.880751e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0395970	MtrunA17_Chr3g0082161	92.734	289	7	1	89	377	10	284	0.0	544
Msa0395970	MtrunA17_Chr1g0164511	92.042	289	9	1	89	377	10	284	0.0	541
Msa0395970	MtrunA17_Chr1g0164501	90.558	233	8	1	145	377	1	219	1.13e-152	429
Msa0395970	MtrunA17_Chr8g0353321	63.309	278	87	2	88	365	36	298	1.58e-127	369
Msa0395970	MtrunA17_Chr3g0082151	78.333	60	9	2	46	104	1	57	4.08e-22	89.0
Msa0395970	MtrunA17_Chr1g0164521	78.333	60	9	2	46	104	1	57	4.08e-22	89.0
Msa0395970	MtrunA17_Chr2g0277381	25.874	286	170	10	92	370	77	327	2.15e-12	67.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0395970	AT1G72050.2	51.807	415	133	4	2	364	3	402	3.16e-143	413
Msa0395970	AT1G72050.3	51.807	415	133	4	2	364	18	417	5.90e-143	412
Msa0395970	AT1G72050.1	51.829	328	92	3	88	364	2	314	2.81e-113	333
Msa0395970	AT1G72050.4	49.310	290	82	2	88	326	2	277	1.30e-93	283

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Find 91 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTTCATTCTGCAGTT+AGG	0.415178	3_2:-13390431	Msa0395970:CDS
AAATGCTTCGGAGGAACTGC+TGG	0.448941	3_2:+13390239	None:intergenic
AAATTCACATTTAAATGCTT+CGG	0.389923	3_2:+13390227	None:intergenic
AAATTTATAGTCATGAATGA+TGG	0.575901	3_2:-13389584	Msa0395970:three_prime_UTR
AACAAGTTCTCTAATTGACT+AGG	0.649660	3_2:+13389741	None:intergenic
AACATGAGCTTTAAGGCACT+CGG	0.529952	3_2:+13390350	None:intergenic
AACTGAAGAAGAATATGAAG+AGG	0.602793	3_2:-13390281	Msa0395970:CDS
AACTGCTGGCTTCATGTGTG+CGG	0.588715	3_2:+13390253	None:intergenic
AAGAAGCTGATGAAGAATTC+AGG	0.351513	3_2:-13389839	Msa0395970:CDS
AAGAATTCAGGTCAAGGCCA+AGG	0.544513	3_2:-13389827	Msa0395970:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATTGTTGTGAAAATTGAG+GGG	+	chr3_2:13389623-13389642	None:intergenic	30.0%
GGAATTGTTGTGAAAATTGA+GGG	+	chr3_2:13389624-13389643	None:intergenic	30.0%
AGGAATTGTTGTGAAAATTG+AGG	+	chr3_2:13389625-13389644	None:intergenic	30.0%
ACAATTCCTTCTATCAACAA+TGG	-	chr3_2:13389636-13389655	Msa0395970:three_prime_UTR	30.0%
ATTGATGTGGGATGTAATTA+GGG	+	chr3_2:13389752-13389771	None:intergenic	30.0%
AATTGATGTGGGATGTAATT+AGG	+	chr3_2:13389753-13389772	None:intergenic	30.0%
AAGAAATTGAGTAACCCATT+GGG	+	chr3_2:13389967-13389986	None:intergenic	30.0%
TAAGAAATTGAGTAACCCAT+TGG	+	chr3_2:13389968-13389987	None:intergenic	30.0%
TATGTGTGATTGATTGTGTA+TGG	-	chr3_2:13390113-13390132	Msa0395970:intron	30.0%
TTTGAAAGCTAATACTTGTG+AGG	-	chr3_2:13390228-13390247	Msa0395970:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	13389540	13392323	13389540	ID=Msa0395970;Name=Msa0395970
chr3_2	mRNA	13389540	13392323	13389540	ID=Msa0395970-mRNA-1;Parent=Msa0395970;Name=Msa0395970-mRNA-1;_AED=0.16;_eAED=0.16;_QI=115\|0.8\|0.66\|1\|0.8\|0.83\|6\|179\|377
chr3_2	exon	13389540	13389871	13389540	ID=Msa0395970-mRNA-1:exon:3148;Parent=Msa0395970-mRNA-1
chr3_2	exon	13389956	13390099	13389956	ID=Msa0395970-mRNA-1:exon:3147;Parent=Msa0395970-mRNA-1
chr3_2	exon	13390200	13390473	13390200	ID=Msa0395970-mRNA-1:exon:3146;Parent=Msa0395970-mRNA-1
chr3_2	exon	13390561	13390856	13390561	ID=Msa0395970-mRNA-1:exon:3145;Parent=Msa0395970-mRNA-1
chr3_2	exon	13391548	13391685	13391548	ID=Msa0395970-mRNA-1:exon:3144;Parent=Msa0395970-mRNA-1
chr3_2	exon	13392080	13392323	13392080	ID=Msa0395970-mRNA-1:exon:3143;Parent=Msa0395970-mRNA-1
chr3_2	five_prime_UTR	13392209	13392323	13392209	ID=Msa0395970-mRNA-1:five_prime_utr;Parent=Msa0395970-mRNA-1
chr3_2	CDS	13392080	13392208	13392080	ID=Msa0395970-mRNA-1:cds;Parent=Msa0395970-mRNA-1
chr3_2	CDS	13391548	13391685	13391548	ID=Msa0395970-mRNA-1:cds;Parent=Msa0395970-mRNA-1
chr3_2	CDS	13390561	13390856	13390561	ID=Msa0395970-mRNA-1:cds;Parent=Msa0395970-mRNA-1
chr3_2	CDS	13390200	13390473	13390200	ID=Msa0395970-mRNA-1:cds;Parent=Msa0395970-mRNA-1
chr3_2	CDS	13389956	13390099	13389956	ID=Msa0395970-mRNA-1:cds;Parent=Msa0395970-mRNA-1
chr3_2	CDS	13389719	13389871	13389719	ID=Msa0395970-mRNA-1:cds;Parent=Msa0395970-mRNA-1
chr3_2	three_prime_UTR	13389540	13389718	13389540	ID=Msa0395970-mRNA-1:three_prime_utr;Parent=Msa0395970-mRNA-1

Gene Sequence

>Msa0395970

ATGGAAGAATCAACCCAAAACGATGAAATCCCAAAATTCAGAGATATTAGACGCTATTACTGCCAATTCTGCGGTATCTGTAGATCCAAAAAAACCCTAATTACATCCCACATCAATTCTGAACACAAGGAGGAGATGGAGAAAGCAAGAGTTGAAACGGAACCTGAATCAGAGGCTGGTTTGAAAGCTAATACTTGTGAGGAATGTGGTGCTAGTTTCAAAAAACATGCTTATTTATTGCAGCATATGCAAAGCCATTCCCTTGAGAGGCCATTTGTATGTAGCCTTGAAGATTGTAAGGCGAGCTACAGAAGAAAGGATCATCTCAATCGTCACCTTCTACAACATCAAGGGAAAACTTTTAAATGTCCTATTGAGGATTGCAAAAGTGGCTTCTCGTTGCAAAGCAATTTGAAAAGACATGTTGAAGAGATGCATGATGAAAGTTCTACCCCTCCGCGTAGTGGTGAAAATCAGAAGCAGTTTGTATGTCCTGAAATTGGCTGTGGAAAGGTTTTCAGATATGCATCACAATTACAGAAGCATGAAGATTCTCATGGCAAATGTTGTCATGATTTTGACGAAAACCTTCATTCTGCAGTTAGGCTCGAAACAATTGAAGTGGTGTGCTTAGAGCCTGGTTGCATGAAATTTTTCACTAATTCCGAGTGCCTTAAAGCTCATGTTAAATCCTGCCATCAATATGTGAACTGTGACACTTGTGGGACTAAGCAACTGAAGAAGAATATGAAGAGGCATCTCCGCACACATGAAGCCAGCAGTTCCTCCGAAGCATTTAAATGTGAATTTGAGGGTTGTGACAGCACATTCTCAACTAAATCTAATCTTCATAAGCATGAGAAAGCCGTACACTTTCAAGTAAAGCCGTTTGTGTGTGGGTTTCCTAATTGTGGAATGAGGTTTGCTTACAAACATGTGAGAGATAATCATGAAAAGACTGCAAAACATCTTTTTGCCCTTGGTGATTTTGAAGAAGCTGATGAAGAATTCAGGTCAAGGCCAAGGGGAGGGGTGAAGAGAAAATGTCCTCCCACAGTAGAAATGTTGGTTAGGAAGAGAGTTACCCCACCTAGTCAATTAGAGAACTTGTTATTTGTGCAGGATTGTCAGTAA

Protein sequence

>Msa0395970

MEESTQNDEIPKFRDIRRYYCQFCGICRSKKTLITSHINSEHKEEMEKARVETEPESEAGLKANTCEECGASFKKHAYLLQHMQSHSLERPFVCSLEDCKASYRRKDHLNRHLLQHQGKTFKCPIEDCKSGFSLQSNLKRHVEEMHDESSTPPRSGENQKQFVCPEIGCGKVFRYASQLQKHEDSHGKCCHDFDENLHSAVRLETIEVVCLEPGCMKFFTNSECLKAHVKSCHQYVNCDTCGTKQLKKNMKRHLRTHEASSSSEAFKCEFEGCDSTFSTKSNLHKHEKAVHFQVKPFVCGFPNCGMRFAYKHVRDNHEKTAKHLFALGDFEEADEEFRSRPRGGVKRKCPPTVEMLVRKRVTPPSQLENLLFVQDCQ*