AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0331530


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0331530 MtrunA17_Chr2g0314431 83.415 205 28 1 1 205 1 199 1.65e-120 339
Msa0331530 MtrunA17_Chr2g0314441 65.714 210 55 4 1 205 1 198 2.75e-89 260
Msa0331530 MtrunA17_Chr8g0359161 47.291 203 83 6 1 188 1 194 2.19e-52 167
Msa0331530 MtrunA17_Chr4g0022761 45.960 198 87 6 1 188 1 188 9.68e-49 157
Msa0331530 MtrunA17_Chr1g0173431 44.776 201 88 6 1 188 1 191 3.10e-48 156
Msa0331530 MtrunA17_Chr3g0082671 42.857 203 91 6 1 188 1 193 6.72e-47 153
Msa0331530 MtrunA17_Chr4g0022751 43.564 202 90 6 1 188 1 192 9.85e-47 152
Msa0331530 MtrunA17_Chr8g0359111 43.564 202 90 6 1 188 1 192 2.61e-46 151
Msa0331530 MtrunA17_Chr3g0082661 42.857 203 91 6 1 188 1 193 7.30e-46 150
Msa0331530 MtrunA17_Chr3g0082641 42.365 203 92 6 1 188 1 193 1.28e-45 150
Msa0331530 MtrunA17_Chr3g0082681 42.365 203 92 6 1 188 1 193 4.27e-45 148
Msa0331530 MtrunA17_Chr3g0082651 42.365 203 92 6 1 188 1 193 3.50e-44 146
Msa0331530 MtrunA17_Chr7g0269461 43.069 202 89 8 1 188 1 190 4.96e-42 140
Msa0331530 MtrunA17_Chr6g0481911 32.727 165 103 5 14 173 10 171 5.38e-18 78.6
Msa0331530 MtrunA17_Chr6g0481941 30.732 205 120 8 14 205 10 205 4.06e-16 73.6
Msa0331530 MtrunA17_Chr6g0482041 31.100 209 108 9 8 189 4 203 4.66e-13 65.5
Msa0331530 MtrunA17_Chr6g0477751 30.055 183 102 8 8 174 4 176 1.62e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0331530 AT1G17860.1 29.187 209 120 8 11 205 1 195 5.78e-14 67.8

Find 52 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTAATTTGCATGAAAA+TGG 0.244897 2_4:-47310323 Msa0331530:CDS
ACAATCTACGGAATGGAAAT+TGG 0.250660 2_4:-47310498 Msa0331530:CDS
AGTGACACGGCCTCCGATAT+CGG 0.290496 2_4:+47310682 None:intergenic
GTTTCTTGACCCACATATAA+AGG 0.297981 2_4:+47310635 None:intergenic
CGGAATGGAAATTGGGTGAT+AGG 0.325123 2_4:-47310490 Msa0331530:CDS
ATACAGTTGTTAAGGTGAAT+AGG 0.332362 2_4:-47310559 Msa0331530:CDS
CAAGCCTTAGCCTTATGTTA+TGG 0.345426 2_4:-47310814 Msa0331530:CDS
GGTCAAGAAACTACAGATAT+TGG 0.348677 2_4:-47310623 Msa0331530:CDS
TACAGTTGTTAAGGTGAATA+GGG 0.357658 2_4:-47310558 Msa0331530:CDS
AAGACTCCACTTGGAATTGA+AGG 0.410273 2_4:-47310404 Msa0331530:CDS
AAGGTTAATGTTCTCAAGGT+TGG 0.412184 2_4:-47310260 Msa0331530:CDS
AGGTTAATGTTCTCAAGGTT+GGG 0.412594 2_4:-47310259 Msa0331530:CDS
AACAGAAGATACAGTTGTTA+AGG 0.418077 2_4:-47310567 Msa0331530:CDS
CGGTTCATGCCCTTTATATG+TGG 0.431349 2_4:-47310645 Msa0331530:CDS
CTGATCTTATGTAATACTTC+CGG 0.434280 2_4:+47310711 None:intergenic
TTTCTTGACCCACATATAAA+GGG 0.438692 2_4:+47310636 None:intergenic
AATTGGGTGATAGGGACAAT+AGG 0.441749 2_4:-47310481 Msa0331530:CDS
GTGACACGGCCTCCGATATC+GGG 0.449326 2_4:+47310683 None:intergenic
CAATCTACGGAATGGAAATT+GGG 0.450660 2_4:-47310497 Msa0331530:CDS
TTCAAGTTGTGGACAATCTA+CGG 0.461337 2_4:-47310510 Msa0331530:CDS
AGATCAGATGTACCCGATAT+CGG 0.472186 2_4:-47310695 Msa0331530:CDS
TTTGATCAATCTTGTTGTCA+TGG 0.481185 2_4:+47310841 None:intergenic
AGGTTGTGCTAATGTTGGTA+TGG 0.501409 2_4:+47310781 None:intergenic
TCATGAAAAGCCATAACATA+AGG 0.507153 2_4:+47310804 None:intergenic
ACGGTTAATTGCGGTACGGT+TGG 0.512688 2_4:-47310341 Msa0331530:CDS
GGAAGGAGGCTTATTACAGC+GGG 0.513610 2_4:-47310455 Msa0331530:CDS
GTCACTTTGATAAATAGAAA+CGG 0.522150 2_4:-47310665 Msa0331530:CDS
GGTGATAGGGACAATAGGAA+TGG 0.534005 2_4:-47310476 Msa0331530:CDS
GGAATGGAAATTGGGTGATA+GGG 0.534497 2_4:-47310489 Msa0331530:CDS
GTCGTCCTCTTGAGAGTGGC+CGG 0.536921 2_4:-47310730 Msa0331530:CDS
CTTATTACAGCGGGGAGTGA+TGG 0.543645 2_4:-47310446 Msa0331530:CDS
TGGTATTAATAATGTTCTCA+AGG 0.548464 2_4:-47310279 Msa0331530:CDS
TCTCAAGGTTAATGTTCTCA+AGG 0.553729 2_4:-47310264 Msa0331530:CDS
ACTGTATCTTCTGTTGCCAT+AGG 0.571927 2_4:+47310575 None:intergenic
ATAGGGACAATAGGAATGGA+AGG 0.576527 2_4:-47310472 Msa0331530:CDS
GGTTCATGCCCTTTATATGT+GGG 0.576927 2_4:-47310644 Msa0331530:CDS
ACCGCAATTAACCGTTTCCG+AGG 0.595491 2_4:+47310349 None:intergenic
GTTGTGGACAATCTACGGAA+TGG 0.597660 2_4:-47310505 Msa0331530:CDS
AAAGCCATAACATAAGGCTA+AGG 0.601835 2_4:+47310810 None:intergenic
TGGAAGGAGGCTTATTACAG+CGG 0.611644 2_4:-47310456 Msa0331530:CDS
TACTTCCGGCCACTCTCAAG+AGG 0.616975 2_4:+47310725 None:intergenic
TTCTATTTATCAAAGTGACA+CGG 0.619288 2_4:+47310669 None:intergenic
ACACGTCGTCCTCTTGAGAG+TGG 0.635863 2_4:-47310734 Msa0331530:CDS
CCGCAATTAACCGTTTCCGA+GGG 0.637522 2_4:+47310350 None:intergenic
AAAACACCTTCAATTCCAAG+TGG 0.642111 2_4:+47310398 None:intergenic
CCCTCGGAAACGGTTAATTG+CGG 0.663767 2_4:-47310350 Msa0331530:CDS
GAAACTACAGATATTGGACA+AGG 0.666365 2_4:-47310617 Msa0331530:CDS
GGAAACGGTTAATTGCGGTA+CGG 0.670786 2_4:-47310345 Msa0331530:CDS
TCAGATGTACCCGATATCGG+AGG 0.696816 2_4:-47310692 Msa0331530:CDS
GGGACAATAGGAATGGAAGG+AGG 0.712776 2_4:-47310469 Msa0331530:CDS
AAACTACAGATATTGGACAA+GGG 0.717751 2_4:-47310616 Msa0331530:CDS
GAAGGAGGCTTATTACAGCG+GGG 0.754937 2_4:-47310454 Msa0331530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGGTAATTATAATATT+AGG - chr2_4:47310704-47310723 Msa0331530:CDS 10.0%
!!! TGAAAATGGTAAGATTTTAT+TGG - chr2_4:47310774-47310793 Msa0331530:CDS 20.0%
! TTCTATTTATCAAAGTGACA+CGG + chr2_4:47310417-47310436 None:intergenic 25.0%
!! GTCACTTTGATAAATAGAAA+CGG - chr2_4:47310418-47310437 Msa0331530:CDS 25.0%
!!! ATCAGTTATTTTTACTCCTA+TGG - chr2_4:47310492-47310511 Msa0331530:CDS 25.0%
! TGGTATTAATAATGTTCTCA+AGG - chr2_4:47310804-47310823 Msa0331530:CDS 25.0%
TCATGAAAAGCCATAACATA+AGG + chr2_4:47310282-47310301 None:intergenic 30.0%
! TCAAGAACTTCTTCATTTTC+AGG + chr2_4:47310325-47310344 None:intergenic 30.0%
CTGATCTTATGTAATACTTC+CGG + chr2_4:47310375-47310394 None:intergenic 30.0%
TTTCTTGACCCACATATAAA+GGG + chr2_4:47310450-47310469 None:intergenic 30.0%
AAACTACAGATATTGGACAA+GGG - chr2_4:47310467-47310486 Msa0331530:CDS 30.0%
AACAGAAGATACAGTTGTTA+AGG - chr2_4:47310516-47310535 Msa0331530:CDS 30.0%
ATACAGTTGTTAAGGTGAAT+AGG - chr2_4:47310524-47310543 Msa0331530:CDS 30.0%
TACAGTTGTTAAGGTGAATA+GGG - chr2_4:47310525-47310544 Msa0331530:CDS 30.0%
! TAAGGTGAATAGGGATTTTA+AGG - chr2_4:47310534-47310553 Msa0331530:CDS 30.0%
!! TAATATTAGGTTTTGTCCCT+CGG - chr2_4:47310717-47310736 Msa0331530:CDS 30.0%
!! GTTGGTAATTTGCATGAAAA+TGG - chr2_4:47310760-47310779 Msa0331530:CDS 30.0%
!!! TGGTAAGATTTTATTGGCTT+TGG - chr2_4:47310780-47310799 Msa0331530:CDS 30.0%
!!! AAGATTTTATTGGCTTTGGA+TGG - chr2_4:47310784-47310803 Msa0331530:CDS 30.0%
AAAGCCATAACATAAGGCTA+AGG + chr2_4:47310276-47310295 None:intergenic 35.0%
!!! TTTTCAGGTTGTGCTAATGT+TGG + chr2_4:47310310-47310329 None:intergenic 35.0%
GTTTCTTGACCCACATATAA+AGG + chr2_4:47310451-47310470 None:intergenic 35.0%
GGTCAAGAAACTACAGATAT+TGG - chr2_4:47310460-47310479 Msa0331530:CDS 35.0%
GAAACTACAGATATTGGACA+AGG - chr2_4:47310466-47310485 Msa0331530:CDS 35.0%
!!! TTTTCAGCTTCTTCAAGTTG+TGG - chr2_4:47310562-47310581 Msa0331530:CDS 35.0%
!! TTCAAGTTGTGGACAATCTA+CGG - chr2_4:47310573-47310592 Msa0331530:CDS 35.0%
ACAATCTACGGAATGGAAAT+TGG - chr2_4:47310585-47310604 Msa0331530:CDS 35.0%
CAATCTACGGAATGGAAATT+GGG - chr2_4:47310586-47310605 Msa0331530:CDS 35.0%
!! AGAATTTTGAAGACTCCACT+TGG - chr2_4:47310670-47310689 Msa0331530:CDS 35.0%
AAAACACCTTCAATTCCAAG+TGG + chr2_4:47310688-47310707 None:intergenic 35.0%
!!! CTTGGAATTGAAGGTGTTTT+AGG - chr2_4:47310688-47310707 Msa0331530:CDS 35.0%
TCTCAAGGTTAATGTTCTCA+AGG - chr2_4:47310819-47310838 Msa0331530:CDS 35.0%
AAGGTTAATGTTCTCAAGGT+TGG - chr2_4:47310823-47310842 Msa0331530:CDS 35.0%
AGGTTAATGTTCTCAAGGTT+GGG - chr2_4:47310824-47310843 Msa0331530:CDS 35.0%
!! CAAGCCTTAGCCTTATGTTA+TGG - chr2_4:47310269-47310288 Msa0331530:CDS 40.0%
AGGTTGTGCTAATGTTGGTA+TGG + chr2_4:47310305-47310324 None:intergenic 40.0%
AGATCAGATGTACCCGATAT+CGG - chr2_4:47310388-47310407 Msa0331530:CDS 40.0%
GGTTCATGCCCTTTATATGT+GGG - chr2_4:47310439-47310458 Msa0331530:CDS 40.0%
ACTGTATCTTCTGTTGCCAT+AGG + chr2_4:47310511-47310530 None:intergenic 40.0%
GGAATGGAAATTGGGTGATA+GGG - chr2_4:47310594-47310613 Msa0331530:CDS 40.0%
AATTGGGTGATAGGGACAAT+AGG - chr2_4:47310602-47310621 Msa0331530:CDS 40.0%
ATAGGGACAATAGGAATGGA+AGG - chr2_4:47310611-47310630 Msa0331530:CDS 40.0%
AAGACTCCACTTGGAATTGA+AGG - chr2_4:47310679-47310698 Msa0331530:CDS 40.0%
CGGTTCATGCCCTTTATATG+TGG - chr2_4:47310438-47310457 Msa0331530:CDS 45.0%
GTTGTGGACAATCTACGGAA+TGG - chr2_4:47310578-47310597 Msa0331530:CDS 45.0%
CGGAATGGAAATTGGGTGAT+AGG - chr2_4:47310593-47310612 Msa0331530:CDS 45.0%
GGTGATAGGGACAATAGGAA+TGG - chr2_4:47310607-47310626 Msa0331530:CDS 45.0%
TGGAAGGAGGCTTATTACAG+CGG - chr2_4:47310627-47310646 Msa0331530:CDS 45.0%
!! TAGGTTTTGTCCCTCGGAAA+CGG - chr2_4:47310723-47310742 Msa0331530:CDS 45.0%
GGAAACGGTTAATTGCGGTA+CGG - chr2_4:47310738-47310757 Msa0331530:CDS 45.0%
TCAGATGTACCCGATATCGG+AGG - chr2_4:47310391-47310410 Msa0331530:CDS 50.0%
GGGACAATAGGAATGGAAGG+AGG - chr2_4:47310614-47310633 Msa0331530:CDS 50.0%
GGAAGGAGGCTTATTACAGC+GGG - chr2_4:47310628-47310647 Msa0331530:CDS 50.0%
GAAGGAGGCTTATTACAGCG+GGG - chr2_4:47310629-47310648 Msa0331530:CDS 50.0%
CTTATTACAGCGGGGAGTGA+TGG - chr2_4:47310637-47310656 Msa0331530:CDS 50.0%
CCGCAATTAACCGTTTCCGA+GGG + chr2_4:47310736-47310755 None:intergenic 50.0%
CCCTCGGAAACGGTTAATTG+CGG - chr2_4:47310733-47310752 Msa0331530:CDS 50.0%
ACCGCAATTAACCGTTTCCG+AGG + chr2_4:47310737-47310756 None:intergenic 50.0%
ACGGTTAATTGCGGTACGGT+TGG - chr2_4:47310742-47310761 Msa0331530:CDS 50.0%
ACACGTCGTCCTCTTGAGAG+TGG - chr2_4:47310349-47310368 Msa0331530:CDS 55.0%
TACTTCCGGCCACTCTCAAG+AGG + chr2_4:47310361-47310380 None:intergenic 55.0%
AGTGACACGGCCTCCGATAT+CGG + chr2_4:47310404-47310423 None:intergenic 55.0%
GTCGTCCTCTTGAGAGTGGC+CGG - chr2_4:47310353-47310372 Msa0331530:CDS 60.0%
GTGACACGGCCTCCGATATC+GGG + chr2_4:47310403-47310422 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 47310244 47310861 47310244 ID=Msa0331530;Name=Msa0331530
chr2_4 mRNA 47310244 47310861 47310244 ID=Msa0331530-mRNA-1;Parent=Msa0331530;Name=Msa0331530-mRNA-1;_AED=0.19;_eAED=0.20;_QI=0|-1|0|1|-1|1|1|0|205
chr2_4 exon 47310244 47310861 47310244 ID=Msa0331530-mRNA-1:exon:14286;Parent=Msa0331530-mRNA-1
chr2_4 CDS 47310244 47310861 47310244 ID=Msa0331530-mRNA-1:cds;Parent=Msa0331530-mRNA-1
Gene Sequence

>Msa0331530

ATGACAACAAGATTGATCAAAATCACAAGCCTTAGCCTTATGTTATGGCTTTTCATGACCATACCAACATTAGCACAACCTGAAAATGAAGAAGTTCTTGACTTAACACGTCGTCCTCTTGAGAGTGGCCGGAAGTATTACATAAGATCAGATGTACCCGATATCGGAGGCCGTGTCACTTTGATAAATAGAAACGGTTCATGCCCTTTATATGTGGGTCAAGAAACTACAGATATTGGACAAGGGTTATCAGTTATTTTTACTCCTATGGCAACAGAAGATACAGTTGTTAAGGTGAATAGGGATTTTAAGGTGAAATTTTCAGCTTCTTCAAGTTGTGGACAATCTACGGAATGGAAATTGGGTGATAGGGACAATAGGAATGGAAGGAGGCTTATTACAGCGGGGAGTGATGGTAGTTATTTTAGAATTTTGAAGACTCCACTTGGAATTGAAGGTGTTTTAGGTAATTATAATATTAGGTTTTGTCCCTCGGAAACGGTTAATTGCGGTACGGTTGGTAATTTGCATGAAAATGGTAAGATTTTATTGGCTTTGGATGGTATTAATAATGTTCTCAAGGTTAATGTTCTCAAGGTTGGGTTCGAGAGAGCGTAA

Protein sequence

>Msa0331530

MTTRLIKITSLSLMLWLFMTIPTLAQPENEEVLDLTRRPLESGRKYYIRSDVPDIGGRVTLINRNGSCPLYVGQETTDIGQGLSVIFTPMATEDTVVKVNRDFKVKFSASSSCGQSTEWKLGDRDNRNGRRLITAGSDGSYFRILKTPLGIEGVLGNYNIRFCPSETVNCGTVGNLHENGKILLALDGINNVLKVNVLKVGFERA*