AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0331540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0331540 MtrunA17_Chr2g0314431 83.415 205 28 1 1 205 1 199 5.57e-121 340
Msa0331540 MtrunA17_Chr2g0314441 66.190 210 54 4 1 205 1 198 1.12e-89 261
Msa0331540 MtrunA17_Chr8g0359161 47.291 203 83 6 1 188 1 194 2.19e-52 167
Msa0331540 MtrunA17_Chr4g0022761 45.960 198 87 6 1 188 1 188 9.68e-49 157
Msa0331540 MtrunA17_Chr1g0173431 44.776 201 88 6 1 188 1 191 3.10e-48 156
Msa0331540 MtrunA17_Chr3g0082671 43.350 203 90 6 1 188 1 193 2.13e-47 154
Msa0331540 MtrunA17_Chr4g0022751 43.564 202 90 6 1 188 1 192 9.85e-47 152
Msa0331540 MtrunA17_Chr3g0082661 43.350 203 90 6 1 188 1 193 2.31e-46 152
Msa0331540 MtrunA17_Chr8g0359111 43.564 202 90 6 1 188 1 192 4.08e-46 151
Msa0331540 MtrunA17_Chr3g0082641 42.857 203 91 6 1 188 1 193 4.11e-46 151
Msa0331540 MtrunA17_Chr3g0082681 42.857 203 91 6 1 188 1 193 1.35e-45 149
Msa0331540 MtrunA17_Chr3g0082651 42.857 203 91 6 1 188 1 193 1.11e-44 147
Msa0331540 MtrunA17_Chr7g0269461 44.059 202 87 8 1 188 1 190 2.45e-43 144
Msa0331540 MtrunA17_Chr6g0481911 32.727 165 103 5 14 173 10 171 5.05e-18 78.6
Msa0331540 MtrunA17_Chr6g0481941 30.732 205 120 8 14 205 10 205 6.46e-16 72.8
Msa0331540 MtrunA17_Chr6g0482041 30.622 209 109 9 8 189 4 203 8.73e-13 64.7
Msa0331540 MtrunA17_Chr6g0477751 29.050 179 109 7 8 174 4 176 8.19e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0331540 AT1G17860.1 29.187 209 120 8 11 205 1 195 4.75e-14 68.2

Find 50 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTAATTTGCATGAAAA+TGG 0.278573 2_4:-47459906 Msa0331540:CDS
ACAATCTACAGAATGGAAAT+TGG 0.284846 2_4:-47460081 Msa0331540:CDS
AGTGACACGGCCTCCGATAT+CGG 0.290496 2_4:+47460265 None:intergenic
GTTTCTTGACCCACATATAA+AGG 0.297981 2_4:+47460218 None:intergenic
ATACAGTTGTTAAGGTGAAT+AGG 0.332362 2_4:-47460142 Msa0331540:CDS
CAGAATGGAAATTGGGTGAT+AGG 0.341031 2_4:-47460073 Msa0331540:CDS
CAAGCCTTAGCCTTATGTTA+TGG 0.345426 2_4:-47460397 Msa0331540:CDS
GGTCAAGAAACTACAGATAT+TGG 0.348677 2_4:-47460206 Msa0331540:CDS
TACAGTTGTTAAGGTGAATA+GGG 0.357658 2_4:-47460141 Msa0331540:CDS
AAGACTCCACTTGGAATTGA+AGG 0.410273 2_4:-47459987 Msa0331540:CDS
AAGGTTAATGTTCTCAAGGT+TGG 0.412184 2_4:-47459843 Msa0331540:CDS
AGGTTAATGTTCTCAAGGTT+GGG 0.412594 2_4:-47459842 Msa0331540:CDS
AACAGAAGATACAGTTGTTA+AGG 0.418077 2_4:-47460150 Msa0331540:CDS
CGGTTCATGCCCTTTATATG+TGG 0.431349 2_4:-47460228 Msa0331540:CDS
CAATCTACAGAATGGAAATT+GGG 0.431621 2_4:-47460080 Msa0331540:CDS
CTGATCTTATGTAATACTTC+CGG 0.434280 2_4:+47460294 None:intergenic
TTTCTTGACCCACATATAAA+GGG 0.438692 2_4:+47460219 None:intergenic
GTGACACGGCCTCCGATATC+GGG 0.449326 2_4:+47460266 None:intergenic
AGATCAGATGTACCCGATAT+CGG 0.472186 2_4:-47460278 Msa0331540:CDS
AATTGGGTGATAGGGACAAT+AGG 0.474158 2_4:-47460064 Msa0331540:CDS
TTTGATCAATCTTGTTGTCA+TGG 0.481185 2_4:+47460424 None:intergenic
AGGTTGTGCTAATGTTGGTA+TGG 0.501409 2_4:+47460364 None:intergenic
TCATGAAAAGCCATAACATA+AGG 0.507153 2_4:+47460387 None:intergenic
AGAATGGAAATTGGGTGATA+GGG 0.514805 2_4:-47460072 Msa0331540:CDS
GGAAGGAGGCTTATTACAGC+GGG 0.521625 2_4:-47460038 Msa0331540:CDS
GTCACTTTGATAAATAGAAA+CGG 0.522150 2_4:-47460248 Msa0331540:CDS
GTCGTCCTCTTGAGAGTGGC+CGG 0.536921 2_4:-47460313 Msa0331540:CDS
CTTATTACAGCGGGGAGTGA+TGG 0.543645 2_4:-47460029 Msa0331540:CDS
TGGTATTAATAATGTTCTCA+AGG 0.548464 2_4:-47459862 Msa0331540:CDS
ATTGTGGACAATCTACAGAA+TGG 0.552740 2_4:-47460088 Msa0331540:CDS
TCTCAAGGTTAATGTTCTCA+AGG 0.553729 2_4:-47459847 Msa0331540:CDS
ACGGTTAATTGCGGTACTGT+TGG 0.557530 2_4:-47459924 Msa0331540:CDS
ACTGTATCTTCTGTTGCCAT+AGG 0.571927 2_4:+47460158 None:intergenic
GGTTCATGCCCTTTATATGT+GGG 0.576927 2_4:-47460227 Msa0331540:CDS
ACCGCAATTAACCGTTTCCG+AGG 0.585521 2_4:+47459932 None:intergenic
CGGAAGGAGGCTTATTACAG+CGG 0.595232 2_4:-47460039 Msa0331540:CDS
ATAGGGACAATAGGAGCGGA+AGG 0.600182 2_4:-47460055 Msa0331540:CDS
AAAGCCATAACATAAGGCTA+AGG 0.601835 2_4:+47460393 None:intergenic
TACTTCCGGCCACTCTCAAG+AGG 0.616975 2_4:+47460308 None:intergenic
TTCTATTTATCAAAGTGACA+CGG 0.619288 2_4:+47460252 None:intergenic
ACACGTCGTCCTCTTGAGAG+TGG 0.635863 2_4:-47460317 Msa0331540:CDS
AAAACACCTTCAATTCCAAG+TGG 0.642111 2_4:+47459981 None:intergenic
CCGCAATTAACCGTTTCCGA+GGG 0.650030 2_4:+47459933 None:intergenic
CCCTCGGAAACGGTTAATTG+CGG 0.663767 2_4:-47459933 Msa0331540:CDS
GAAACTACAGATATTGGACA+AGG 0.666365 2_4:-47460200 Msa0331540:CDS
GGGACAATAGGAGCGGAAGG+AGG 0.666908 2_4:-47460052 Msa0331540:CDS
GGTGATAGGGACAATAGGAG+CGG 0.692836 2_4:-47460059 Msa0331540:CDS
TCAGATGTACCCGATATCGG+AGG 0.696816 2_4:-47460275 Msa0331540:CDS
AAACTACAGATATTGGACAA+GGG 0.717751 2_4:-47460199 Msa0331540:CDS
GAAGGAGGCTTATTACAGCG+GGG 0.754937 2_4:-47460037 Msa0331540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGGTAATTATAATATT+AGG - chr2_4:47460287-47460306 Msa0331540:CDS 10.0%
!!! TGAAAATGGTAAGATTTTAT+TGG - chr2_4:47460357-47460376 Msa0331540:CDS 20.0%
! TTCTATTTATCAAAGTGACA+CGG + chr2_4:47460000-47460019 None:intergenic 25.0%
!! GTCACTTTGATAAATAGAAA+CGG - chr2_4:47460001-47460020 Msa0331540:CDS 25.0%
!!! ATCAGTTATTTTTACTCCTA+TGG - chr2_4:47460075-47460094 Msa0331540:CDS 25.0%
! TGGTATTAATAATGTTCTCA+AGG - chr2_4:47460387-47460406 Msa0331540:CDS 25.0%
TCATGAAAAGCCATAACATA+AGG + chr2_4:47459865-47459884 None:intergenic 30.0%
! TCAAGAACTTCTTCATTTTC+AGG + chr2_4:47459908-47459927 None:intergenic 30.0%
CTGATCTTATGTAATACTTC+CGG + chr2_4:47459958-47459977 None:intergenic 30.0%
TTTCTTGACCCACATATAAA+GGG + chr2_4:47460033-47460052 None:intergenic 30.0%
AAACTACAGATATTGGACAA+GGG - chr2_4:47460050-47460069 Msa0331540:CDS 30.0%
AACAGAAGATACAGTTGTTA+AGG - chr2_4:47460099-47460118 Msa0331540:CDS 30.0%
ATACAGTTGTTAAGGTGAAT+AGG - chr2_4:47460107-47460126 Msa0331540:CDS 30.0%
TACAGTTGTTAAGGTGAATA+GGG - chr2_4:47460108-47460127 Msa0331540:CDS 30.0%
! TAAGGTGAATAGGGATTTTA+AGG - chr2_4:47460117-47460136 Msa0331540:CDS 30.0%
! TTTTCAGCTTCTTCAAATTG+TGG - chr2_4:47460145-47460164 Msa0331540:CDS 30.0%
ACAATCTACAGAATGGAAAT+TGG - chr2_4:47460168-47460187 Msa0331540:CDS 30.0%
CAATCTACAGAATGGAAATT+GGG - chr2_4:47460169-47460188 Msa0331540:CDS 30.0%
!! TAATATTAGGTTTTGTCCCT+CGG - chr2_4:47460300-47460319 Msa0331540:CDS 30.0%
!! GTTGGTAATTTGCATGAAAA+TGG - chr2_4:47460343-47460362 Msa0331540:CDS 30.0%
!!! TGGTAAGATTTTATTGGCTT+TGG - chr2_4:47460363-47460382 Msa0331540:CDS 30.0%
!!! AAGATTTTATTGGCTTTGGA+TGG - chr2_4:47460367-47460386 Msa0331540:CDS 30.0%
AAAGCCATAACATAAGGCTA+AGG + chr2_4:47459859-47459878 None:intergenic 35.0%
!!! TTTTCAGGTTGTGCTAATGT+TGG + chr2_4:47459893-47459912 None:intergenic 35.0%
GTTTCTTGACCCACATATAA+AGG + chr2_4:47460034-47460053 None:intergenic 35.0%
GGTCAAGAAACTACAGATAT+TGG - chr2_4:47460043-47460062 Msa0331540:CDS 35.0%
GAAACTACAGATATTGGACA+AGG - chr2_4:47460049-47460068 Msa0331540:CDS 35.0%
ATTGTGGACAATCTACAGAA+TGG - chr2_4:47460161-47460180 Msa0331540:CDS 35.0%
AGAATGGAAATTGGGTGATA+GGG - chr2_4:47460177-47460196 Msa0331540:CDS 35.0%
!! AGAATTTTGAAGACTCCACT+TGG - chr2_4:47460253-47460272 Msa0331540:CDS 35.0%
AAAACACCTTCAATTCCAAG+TGG + chr2_4:47460271-47460290 None:intergenic 35.0%
!!! CTTGGAATTGAAGGTGTTTT+AGG - chr2_4:47460271-47460290 Msa0331540:CDS 35.0%
TCTCAAGGTTAATGTTCTCA+AGG - chr2_4:47460402-47460421 Msa0331540:CDS 35.0%
AAGGTTAATGTTCTCAAGGT+TGG - chr2_4:47460406-47460425 Msa0331540:CDS 35.0%
AGGTTAATGTTCTCAAGGTT+GGG - chr2_4:47460407-47460426 Msa0331540:CDS 35.0%
!! CAAGCCTTAGCCTTATGTTA+TGG - chr2_4:47459852-47459871 Msa0331540:CDS 40.0%
AGGTTGTGCTAATGTTGGTA+TGG + chr2_4:47459888-47459907 None:intergenic 40.0%
AGATCAGATGTACCCGATAT+CGG - chr2_4:47459971-47459990 Msa0331540:CDS 40.0%
GGTTCATGCCCTTTATATGT+GGG - chr2_4:47460022-47460041 Msa0331540:CDS 40.0%
ACTGTATCTTCTGTTGCCAT+AGG + chr2_4:47460094-47460113 None:intergenic 40.0%
CAGAATGGAAATTGGGTGAT+AGG - chr2_4:47460176-47460195 Msa0331540:CDS 40.0%
AATTGGGTGATAGGGACAAT+AGG - chr2_4:47460185-47460204 Msa0331540:CDS 40.0%
AAGACTCCACTTGGAATTGA+AGG - chr2_4:47460262-47460281 Msa0331540:CDS 40.0%
CGGTTCATGCCCTTTATATG+TGG - chr2_4:47460021-47460040 Msa0331540:CDS 45.0%
!! TAGGTTTTGTCCCTCGGAAA+CGG - chr2_4:47460306-47460325 Msa0331540:CDS 45.0%
ACGGTTAATTGCGGTACTGT+TGG - chr2_4:47460325-47460344 Msa0331540:CDS 45.0%
TCAGATGTACCCGATATCGG+AGG - chr2_4:47459974-47459993 Msa0331540:CDS 50.0%
GGTGATAGGGACAATAGGAG+CGG - chr2_4:47460190-47460209 Msa0331540:CDS 50.0%
ATAGGGACAATAGGAGCGGA+AGG - chr2_4:47460194-47460213 Msa0331540:CDS 50.0%
CGGAAGGAGGCTTATTACAG+CGG - chr2_4:47460210-47460229 Msa0331540:CDS 50.0%
GGAAGGAGGCTTATTACAGC+GGG - chr2_4:47460211-47460230 Msa0331540:CDS 50.0%
GAAGGAGGCTTATTACAGCG+GGG - chr2_4:47460212-47460231 Msa0331540:CDS 50.0%
CTTATTACAGCGGGGAGTGA+TGG - chr2_4:47460220-47460239 Msa0331540:CDS 50.0%
CCGCAATTAACCGTTTCCGA+GGG + chr2_4:47460319-47460338 None:intergenic 50.0%
CCCTCGGAAACGGTTAATTG+CGG - chr2_4:47460316-47460335 Msa0331540:CDS 50.0%
ACCGCAATTAACCGTTTCCG+AGG + chr2_4:47460320-47460339 None:intergenic 50.0%
ACACGTCGTCCTCTTGAGAG+TGG - chr2_4:47459932-47459951 Msa0331540:CDS 55.0%
TACTTCCGGCCACTCTCAAG+AGG + chr2_4:47459944-47459963 None:intergenic 55.0%
AGTGACACGGCCTCCGATAT+CGG + chr2_4:47459987-47460006 None:intergenic 55.0%
GTCGTCCTCTTGAGAGTGGC+CGG - chr2_4:47459936-47459955 Msa0331540:CDS 60.0%
GTGACACGGCCTCCGATATC+GGG + chr2_4:47459986-47460005 None:intergenic 60.0%
GGGACAATAGGAGCGGAAGG+AGG - chr2_4:47460197-47460216 Msa0331540:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 47459827 47460444 47459827 ID=Msa0331540;Name=Msa0331540
chr2_4 mRNA 47459827 47460444 47459827 ID=Msa0331540-mRNA-1;Parent=Msa0331540;Name=Msa0331540-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|-1|0|1|-1|1|1|0|205
chr2_4 exon 47459827 47460444 47459827 ID=Msa0331540-mRNA-1:exon:14287;Parent=Msa0331540-mRNA-1
chr2_4 CDS 47459827 47460444 47459827 ID=Msa0331540-mRNA-1:cds;Parent=Msa0331540-mRNA-1
Gene Sequence

>Msa0331540

ATGACAACAAGATTGATCAAAATCACAAGCCTTAGCCTTATGTTATGGCTTTTCATGACCATACCAACATTAGCACAACCTGAAAATGAAGAAGTTCTTGACTTAACACGTCGTCCTCTTGAGAGTGGCCGGAAGTATTACATAAGATCAGATGTACCCGATATCGGAGGCCGTGTCACTTTGATAAATAGAAACGGTTCATGCCCTTTATATGTGGGTCAAGAAACTACAGATATTGGACAAGGGTTATCAGTTATTTTTACTCCTATGGCAACAGAAGATACAGTTGTTAAGGTGAATAGGGATTTTAAGGTGAAATTTTCAGCTTCTTCAAATTGTGGACAATCTACAGAATGGAAATTGGGTGATAGGGACAATAGGAGCGGAAGGAGGCTTATTACAGCGGGGAGTGATGGTAGTTATTTTAGAATTTTGAAGACTCCACTTGGAATTGAAGGTGTTTTAGGTAATTATAATATTAGGTTTTGTCCCTCGGAAACGGTTAATTGCGGTACTGTTGGTAATTTGCATGAAAATGGTAAGATTTTATTGGCTTTGGATGGTATTAATAATGTTCTCAAGGTTAATGTTCTCAAGGTTGGGTTCGAGAGAGCGTAA

Protein sequence

>Msa0331540

MTTRLIKITSLSLMLWLFMTIPTLAQPENEEVLDLTRRPLESGRKYYIRSDVPDIGGRVTLINRNGSCPLYVGQETTDIGQGLSVIFTPMATEDTVVKVNRDFKVKFSASSNCGQSTEWKLGDRDNRSGRRLITAGSDGSYFRILKTPLGIEGVLGNYNIRFCPSETVNCGTVGNLHENGKILLALDGINNVLKVNVLKVGFERA*