AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1357070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1357070 MtrunA17_Chr1g0193131 94.945 633 32 0 1 633 1 633 0.0 1019
Msa1357070 MtrunA17_Chr7g0271411 63.402 194 50 8 318 503 64 244 1.36e-55 191
Msa1357070 MtrunA17_Chr3g0096451 47.799 159 60 4 346 503 60 196 1.29e-36 137
Msa1357070 MtrunA17_Chr5g0443331 53.636 110 50 1 402 510 90 199 3.17e-31 122
Msa1357070 MtrunA17_Chr2g0304061 41.121 214 83 6 331 511 46 249 1.42e-29 119
Msa1357070 MtrunA17_Chr2g0304061 48.031 127 49 2 420 546 158 267 2.39e-25 107
Msa1357070 MtrunA17_Chr8g0378371 60.227 88 33 1 418 505 31 116 1.36e-28 110
Msa1357070 MtrunA17_Chr8g0381081 53.636 110 47 3 405 512 44 151 1.49e-28 112
Msa1357070 MtrunA17_Chr1g0177171 57.609 92 37 2 416 505 402 493 1.26e-27 117
Msa1357070 MtrunA17_Chr5g0401301 25.617 527 254 18 1 502 1 414 2.29e-27 115
Msa1357070 MtrunA17_Chr8g0378361 45.038 131 60 2 419 548 29 148 5.02e-27 108
Msa1357070 MtrunA17_Chr4g0075031 50.485 103 49 2 412 512 374 476 3.31e-26 112
Msa1357070 MtrunA17_Chr8g0378381 49.438 89 44 1 416 503 28 116 1.75e-24 98.6
Msa1357070 MtrunA17_Chr8g0378401 49.398 83 41 1 416 497 28 110 2.88e-22 92.4
Msa1357070 MtrunA17_Chr8g0380231 63.768 69 24 1 429 496 26 94 4.31e-22 91.3
Msa1357070 MtrunA17_Chr3g0121271 46.000 100 52 2 418 515 360 459 7.54e-22 99.4
Msa1357070 MtrunA17_Chr5g0447651 51.613 93 43 2 412 502 22 114 9.53e-22 90.9
Msa1357070 MtrunA17_Chr1g0154991 47.778 90 46 1 419 507 19 108 1.03e-21 90.5
Msa1357070 MtrunA17_Chr3g0120401 50.575 87 42 1 420 505 157 243 1.27e-21 94.4
Msa1357070 MtrunA17_Chr5g0434591 47.917 96 48 2 412 505 352 447 6.69e-21 96.7
Msa1357070 MtrunA17_Chr8g0378621 47.368 95 48 2 420 512 364 458 8.41e-21 96.3
Msa1357070 MtrunA17_Chr6g0487261 38.739 111 65 2 408 515 357 467 1.42e-20 95.5
Msa1357070 MtrunA17_Chr5g0439251 54.444 90 39 2 414 501 376 465 1.74e-20 95.1
Msa1357070 MtrunA17_Chr4g0043511 41.000 100 58 1 418 516 418 517 1.93e-20 95.5
Msa1357070 MtrunA17_Chr3g0110371 40.984 122 70 2 397 516 338 459 8.73e-20 92.8
Msa1357070 MtrunA17_Chr4g0061401 52.941 85 38 2 419 502 23 106 9.41e-20 88.6
Msa1357070 MtrunA17_Chr1g0213651 45.455 88 47 1 416 502 272 359 1.06e-19 91.3
Msa1357070 MtrunA17_Chr4g0040911 49.474 95 46 2 420 512 37 131 1.25e-19 87.0
Msa1357070 MtrunA17_Chr5g0419121 50.000 92 43 2 417 505 19 110 1.27e-19 89.0
Msa1357070 MtrunA17_Chr4g0043151 50.000 88 42 2 420 505 21 108 4.36e-19 85.9
Msa1357070 MtrunA17_Chr4g0076141 47.727 88 44 2 414 499 7 94 5.65e-19 82.4
Msa1357070 MtrunA17_Chr8g0389741 36.441 118 73 2 411 526 359 476 5.79e-19 90.5
Msa1357070 MtrunA17_Chr2g0313891 46.392 97 49 3 408 502 17 112 1.14e-18 82.0
Msa1357070 MtrunA17_Chr4g0076131 46.154 91 47 2 414 502 22 112 1.34e-18 82.0
Msa1357070 MtrunA17_Chr4g0034901 45.133 113 53 4 403 507 353 464 1.35e-18 89.0
Msa1357070 MtrunA17_Chr1g0161011 40.517 116 67 2 418 531 359 474 3.13e-18 88.2
Msa1357070 MtrunA17_Chr8g0339191 39.216 102 55 3 405 504 364 460 1.74e-16 82.4
Msa1357070 MtrunA17_Chr7g0234821 42.478 113 60 5 395 504 350 460 5.27e-16 80.9
Msa1357070 MtrunA17_Chr3g0144531 42.202 109 58 3 403 507 352 459 6.70e-16 80.9
Msa1357070 MtrunA17_Chr7g0223961 38.235 102 62 1 400 500 345 446 4.31e-14 75.1
Msa1357070 MtrunA17_Chr2g0306311 35.849 106 58 5 418 518 397 497 7.56e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1357070 AT1G09460.1 69.048 126 36 2 386 509 101 225 9.43e-44 160
Msa1357070 AT2G30933.1 59.574 94 37 1 418 510 80 173 4.50e-33 127
Msa1357070 AT1G29380.3 64.444 90 31 1 420 508 148 237 1.61e-31 124
Msa1357070 AT1G29380.1 65.476 84 28 1 420 502 148 231 3.06e-30 121
Msa1357070 AT2G30933.2 43.846 130 41 2 368 496 61 159 8.76e-29 112
Msa1357070 AT2G30933.3 43.846 130 41 2 368 496 61 159 8.76e-29 112
Msa1357070 AT1G29380.4 66.667 78 25 1 420 496 148 225 2.09e-28 114
Msa1357070 AT1G29380.2 66.667 78 25 1 420 496 148 225 2.09e-28 114
Msa1357070 AT2G05790.1 53.448 116 49 3 395 505 356 471 2.98e-28 119
Msa1357070 AT1G11820.2 51.515 99 46 2 418 514 379 477 1.59e-25 111
Msa1357070 AT4G13600.1 44.094 127 66 3 420 542 61 186 2.14e-25 105
Msa1357070 AT4G05430.1 44.444 117 63 2 400 514 3 119 2.66e-25 102
Msa1357070 AT4G05430.3 44.167 120 65 2 397 514 25 144 7.08e-25 102
Msa1357070 AT5G55180.1 52.632 95 43 2 413 505 365 459 1.32e-24 107
Msa1357070 AT4G26830.1 35.751 193 110 6 322 505 267 454 1.07e-23 105
Msa1357070 AT3G55430.1 46.739 92 48 1 412 502 358 449 1.27e-23 104
Msa1357070 AT4G26830.2 35.751 193 110 6 322 505 271 458 1.55e-23 104
Msa1357070 AT5G55180.2 55.814 86 36 2 413 496 365 450 2.61e-22 100
Msa1357070 AT5G35740.1 52.747 91 40 3 415 502 25 115 2.89e-22 92.8
Msa1357070 AT2G01630.1 39.535 129 74 3 418 544 358 484 1.57e-21 99.0
Msa1357070 AT4G34480.3 47.674 86 44 1 419 503 252 337 1.57e-21 97.8
Msa1357070 AT1G18650.1 47.778 90 46 1 419 507 20 109 2.05e-21 92.8
Msa1357070 AT4G34480.1 47.674 86 44 1 419 503 363 448 2.58e-21 98.2
Msa1357070 AT3G58100.1 47.872 94 47 2 420 511 41 134 5.73e-21 91.3
Msa1357070 AT1G11820.3 54.321 81 35 2 418 496 379 459 6.89e-21 96.7
Msa1357070 AT3G58100.2 46.939 98 50 2 416 511 113 210 9.03e-21 92.8
Msa1357070 AT1G18650.2 47.191 89 46 1 419 506 20 108 1.36e-20 88.6
Msa1357070 AT4G05430.2 46.078 102 53 2 397 496 25 126 1.52e-20 88.2
Msa1357070 AT1G79480.2 50.000 84 41 1 420 502 310 393 3.67e-20 93.6
Msa1357070 AT1G79480.1 50.000 84 41 1 420 502 311 394 3.68e-20 93.6
Msa1357070 AT1G66250.1 39.474 114 66 3 418 529 366 478 5.61e-20 94.0
Msa1357070 AT2G16230.1 39.806 103 61 1 419 520 365 467 1.14e-19 93.2
Msa1357070 AT5G61130.1 47.674 86 44 1 419 503 20 105 1.34e-19 87.8
Msa1357070 AT1G69295.1 56.338 71 29 2 419 488 20 89 2.44e-19 87.8
Msa1357070 AT1G69295.2 56.338 71 29 2 419 488 16 85 4.25e-19 87.0
Msa1357070 AT5G67460.1 48.235 85 42 2 420 502 293 377 4.48e-19 90.1
Msa1357070 AT5G56590.1 40.777 103 59 2 420 520 369 471 9.90e-19 90.1
Msa1357070 AT1G26450.1 38.312 154 67 6 421 550 22 171 1.06e-18 85.1
Msa1357070 AT5G63225.1 46.512 86 45 1 418 502 23 108 1.44e-18 82.0
Msa1357070 AT1G66870.2 42.529 87 48 2 418 502 51 137 2.53e-18 82.4
Msa1357070 AT1G66870.1 42.529 87 48 2 418 502 24 110 2.80e-18 81.3
Msa1357070 AT5G67460.2 50.633 79 37 2 420 496 293 371 2.89e-18 87.8
Msa1357070 AT4G29360.2 45.977 87 45 2 418 502 399 485 3.02e-18 89.0
Msa1357070 AT4G29360.1 45.977 87 45 2 418 502 389 475 3.10e-18 89.0
Msa1357070 AT4G09462.1 41.758 91 52 1 415 504 24 114 4.45e-18 80.9
Msa1357070 AT5G24318.3 36.697 109 62 2 398 505 217 319 5.20e-18 85.9
Msa1357070 AT5G24318.1 36.697 109 62 2 398 505 356 458 5.86e-18 87.4
Msa1357070 AT4G09465.1 40.659 91 53 1 415 504 26 116 8.58e-18 80.1
Msa1357070 AT5G08000.2 44.262 122 66 2 417 536 37 158 9.35e-18 82.8
Msa1357070 AT4G09464.1 40.659 91 53 1 415 504 26 116 1.05e-17 79.7
Msa1357070 AT4G09467.1 40.659 91 53 1 415 504 26 116 1.05e-17 79.7
Msa1357070 AT3G13560.4 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
Msa1357070 AT3G13560.1 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
Msa1357070 AT3G13560.2 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
Msa1357070 AT3G13560.5 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
Msa1357070 AT3G13560.3 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
Msa1357070 AT4G09466.1 40.659 91 53 1 415 504 26 116 2.22e-17 79.0
Msa1357070 AT2G42930.1 44.872 78 43 0 420 497 40 117 2.94e-17 79.0
Msa1357070 AT4G09090.1 43.023 86 48 1 420 504 31 116 3.21e-17 78.6
Msa1357070 AT2G01630.3 48.148 81 40 2 418 496 358 438 3.55e-17 85.1
Msa1357070 AT1G66852.1 39.535 86 51 1 418 502 24 109 5.49e-17 77.4
Msa1357070 AT3G23770.1 44.118 102 53 4 405 504 374 473 9.58e-17 84.0
Msa1357070 AT5G08000.1 47.674 86 44 1 419 503 20 105 9.67e-17 79.3
Msa1357070 AT1G78520.1 40.000 90 52 2 414 502 26 114 1.68e-16 76.3
Msa1357070 AT2G04910.2 43.182 88 48 2 415 500 10 97 1.87e-16 75.9
Msa1357070 AT5G63240.1 36.275 102 61 2 407 504 27 128 7.02e-16 75.1
Msa1357070 AT5G24318.2 37.000 100 56 2 398 496 356 449 1.32e-15 80.1
Msa1357070 AT5G63230.1 37.363 91 56 1 413 502 19 109 3.98e-15 72.4
Msa1357070 AT1G66855.1 37.931 87 52 2 418 502 24 110 6.17e-15 71.6
Msa1357070 AT5G63250.1 38.776 98 55 3 412 504 33 130 8.63e-15 72.0
Msa1357070 AT4G16165.1 37.931 87 52 2 418 502 24 110 2.06e-14 70.1
Msa1357070 AT2G43670.1 38.298 94 55 3 410 502 26 117 4.18e-14 69.7
Msa1357070 AT5G53600.1 39.535 86 50 2 418 502 27 111 5.33e-14 68.9
Msa1357070 AT2G04910.1 43.678 87 46 3 412 496 8 93 6.93e-14 69.3
Msa1357070 AT2G39640.1 34.091 88 57 1 419 505 459 546 8.52e-14 74.7
Msa1357070 AT2G39640.1 35.417 96 55 2 406 500 351 440 3.27e-12 69.7
Msa1357070 AT2G43660.2 41.667 96 52 4 409 502 26 119 1.34e-13 68.2
Msa1357070 AT2G43660.1 41.667 96 52 4 409 502 25 118 1.62e-13 68.2
Msa1357070 AT3G07320.1 39.216 102 58 3 405 504 358 457 3.75e-13 72.4
Msa1357070 AT2G29735.1 39.785 93 53 2 413 502 24 116 6.48e-13 66.2
Msa1357070 AT4G14080.1 37.255 102 62 2 405 504 374 475 7.24e-13 71.6
Msa1357070 AT1G13830.2 39.130 92 47 3 417 502 32 120 8.64e-13 68.6
Msa1357070 AT1G13830.1 39.326 89 45 3 420 502 21 106 1.29e-12 67.4
Msa1357070 AT2G03505.3 36.667 90 54 2 419 505 20 109 1.50e-12 65.1
Msa1357070 AT2G03505.4 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
Msa1357070 AT2G03505.1 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
Msa1357070 AT2G03505.2 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
Msa1357070 AT5G53610.1 41.463 82 46 2 420 500 28 108 2.30e-12 64.3
Msa1357070 AT5G64790.1 38.144 97 56 4 418 512 367 461 5.66e-12 68.9

Find 170 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATCGCCATCAGTGCTTTC+TGG 0.163697 tig0015882:-9608 Msa1357080:three_prime_UTR
AATATATTCATTAAATTATC+AGG 0.166823 tig0015882:+9579 Msa1357070:three_prime_UTR
TCAGGTACAATGCCAGATTT+TGG 0.194586 tig0015882:+8984 Msa1357070:CDS
CCAACATCTTCAGGCACTTT+TGG 0.216484 tig0015882:+8814 Msa1357070:CDS
TCACTTTCATGCAGGTTCTT+TGG 0.248475 tig0015882:+7089 Msa1357070:intron
TCTCTCTTAATTTCATGTTC+TGG 0.269637 tig0015882:+6993 Msa1357070:CDS
TAGATTACTTTCATTTATAA+AGG 0.277901 tig0015882:+7545 Msa1357070:CDS
TGAGTGGGAAGGAGAGAAAA+AGG 0.289037 tig0015882:-6828 None:intergenic
TTAGGTTGTACAATATATTA+TGG 0.299298 tig0015882:+9299 Msa1357070:three_prime_UTR
GGTGGAGCTGTTGGAATTGA+TGG 0.308099 tig0015882:-8793 None:intergenic
AAGACTATACAGCCAGTAAA+AGG 0.355621 tig0015882:-9065 None:intergenic
GGAGGCGCTGCTATGTTAGT+TGG 0.360601 tig0015882:+8494 Msa1357070:CDS
ATGAAGAAACTTTGCTCTGC+TGG 0.376113 tig0015882:-7158 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.377508 tig0015882:-8197 None:intergenic
ATGATAGAAGATAGCATTGA+TGG 0.377602 tig0015882:+7585 Msa1357070:CDS
TAGCAAATAACTTTAGATAA+AGG 0.378208 tig0015882:-6854 None:intergenic
CCTCCAAAGTCGCAACTTGT+TGG 0.380624 tig0015882:-8476 None:intergenic
GGTGTAATGGTTGGAGTTGT+TGG 0.382964 tig0015882:-8101 None:intergenic
GGTGGAGGTGTTGTTAGATT+TGG 0.383669 tig0015882:-8727 None:intergenic
GGCATTGCAGCTGGATTTGT+AGG 0.384777 tig0015882:-8038 None:intergenic
TGTGGAAGGAACTGCTATAA+TGG 0.388341 tig0015882:-7997 None:intergenic
AACTCAAGCAACCCAGCTTC+AGG 0.391337 tig0015882:+8966 Msa1357070:CDS
TCAGTGTGATTGATATATAC+AGG 0.391997 tig0015882:-6895 None:intergenic
GTCAGAGTTGTTATAATTTG+AGG 0.392142 tig0015882:+9418 Msa1357070:three_prime_UTR
GCTCCAACAAGTTGCGACTT+TGG 0.392668 tig0015882:+8473 Msa1357070:CDS
TACACAATACTCTCTGATAA+TGG 0.394136 tig0015882:+6935 Msa1357070:exon
ATAACTGGTGTTGGATTTGT+AGG 0.394170 tig0015882:-7954 None:intergenic
GCATGGTTCTGCAAAGTAAC+AGG 0.394899 tig0015882:-8413 None:intergenic
GAGCTTTCGTCACAGATCAT+AGG 0.396108 tig0015882:+7190 Msa1357070:CDS
GGAGGTGTTGTTAGATTTGG+TGG 0.400113 tig0015882:-8724 None:intergenic
CGAGTTACTTCTATTGAACT+TGG 0.401043 tig0015882:-7726 None:intergenic
TTGGATTATGCTTGTGGAAA+TGG 0.401736 tig0015882:+8347 Msa1357070:CDS
CAATGTAGTTCAGTGTAAAT+TGG 0.409911 tig0015882:+9396 Msa1357070:three_prime_UTR
CTTCAATGAAAGAACTCAAA+AGG 0.413209 tig0015882:+7829 Msa1357070:CDS
GTAGTTGAAGGAACTGTTAC+TGG 0.413784 tig0015882:-8059 None:intergenic
GAAGGAGCATTTGTGTTTGA+AGG 0.413852 tig0015882:-8260 None:intergenic
GTTGGAGTTGTTGGAGGTAA+AGG 0.413921 tig0015882:-8092 None:intergenic
TTGAAGGAGGTGGCTGTTGA+GGG 0.417203 tig0015882:-8244 None:intergenic
CATTACATGCTGCAGTGTTA+AGG 0.418204 tig0015882:+9201 Msa1357070:three_prime_UTR
CACAAATGCTCCTTCTGCTC+AGG 0.420750 tig0015882:+8268 Msa1357070:CDS
ACAGCTCCACCAACATCTTC+AGG 0.424830 tig0015882:+8805 Msa1357070:CDS
ACCACTGGAACATTTCCTAA+TGG 0.424925 tig0015882:-8218 None:intergenic
GGATAAGATGTTACTGGAGG+TGG 0.426667 tig0015882:-8191 None:intergenic
GCCGATGTTGTGTTTACAAT+AGG 0.430103 tig0015882:-9020 None:intergenic
ACTGTTACTGGCATTGCAGC+TGG 0.430772 tig0015882:-8047 None:intergenic
TGATTGTTCTCAAATACAAC+AGG 0.433295 tig0015882:+8373 Msa1357070:CDS
ACAACTCCTACAACAGTTCC+AGG 0.436358 tig0015882:+8146 Msa1357070:CDS
ACATCTTATCCACCTCCATT+AGG 0.436623 tig0015882:+8203 Msa1357070:CDS
GCACATGTGAAATGCTTTCA+AGG 0.438918 tig0015882:+9661 Msa1357070:three_prime_UTR
CTTTCATGCAGGTTCTTTGG+TGG 0.439102 tig0015882:+7092 Msa1357070:intron
GGAAATTGTGACAGGATTTG+TGG 0.439347 tig0015882:-8015 None:intergenic
GGAGCTATTGTTGGAATAGA+TGG 0.441593 tig0015882:-8748 None:intergenic
GGGCTATCAGACCCAAAATC+TGG 0.444861 tig0015882:-8996 None:intergenic
CACCTATAGAGTTCAGAAAC+AGG 0.446988 tig0015882:+9122 Msa1357070:exon
TTTCATGCAGGTTCTTTGGT+GGG 0.448012 tig0015882:+7093 Msa1357070:intron
TTTGAAGGAGGTGGCTGTTG+AGG 0.448619 tig0015882:-8245 None:intergenic
GGTGGAGCTGTTGACATTGT+TGG 0.450114 tig0015882:-8772 None:intergenic
AGCTTCCCTTCAATCAGCAT+TGG 0.454325 tig0015882:+8328 Msa1357070:CDS
AACTTCCTCAAGACTTGAAG+TGG 0.458266 tig0015882:-7495 None:intergenic
AACCTGTTTCTGAACTCTAT+AGG 0.458686 tig0015882:-9124 None:intergenic
CTGAGGCTTGCAGTGACAAA+TGG 0.467298 tig0015882:-9095 None:intergenic
ATTGTTGGTGGAGCTATTGT+TGG 0.472956 tig0015882:-8757 None:intergenic
AGTTGGTGTGTGGCGAAAAC+AGG 0.475765 tig0015882:+8296 Msa1357070:CDS
AAGTCGCAACTTGTTGGAGC+TGG 0.478203 tig0015882:-8470 None:intergenic
GAGGCAGAAAGAAGCTTCCT+TGG 0.478234 tig0015882:-6958 None:intergenic
TCTGGCATTGTACCTGAAGC+TGG 0.478959 tig0015882:-8978 None:intergenic
GGTGCACCTGGAACTGTTGT+AGG 0.485248 tig0015882:-8152 None:intergenic
GTAGGGTAAACTGTGTTTGT+TGG 0.486254 tig0015882:-7936 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.487201 tig0015882:-8176 None:intergenic
GATAAAGGAGGTGAGTGGGA+AGG 0.489504 tig0015882:-6839 None:intergenic
AGAATCAGCATCCAATGCAT+TGG 0.490685 tig0015882:-9548 None:intergenic
GATATAGAAGCACCTGTCAC+AGG 0.490760 tig0015882:-9496 None:intergenic
GGCTGTTGAGGGTTTACCAC+TGG 0.491135 tig0015882:-8233 None:intergenic
CATAATCCAATGCTGATTGA+AGG 0.493090 tig0015882:-8334 None:intergenic
GTTGATTGTGTAGGTGGTGT+TGG 0.494210 tig0015882:-8685 None:intergenic
TTTGTAGGGGAAATTGTGAC+AGG 0.494725 tig0015882:-8023 None:intergenic
TATCTAGAATATTTCTTCTG+TGG 0.496929 tig0015882:-7902 None:intergenic
AAAATATTCTAAGCAGCTCA+TGG 0.499869 tig0015882:-9278 None:intergenic
AGTGTCTCCTCTCTCATGAT+AGG 0.500543 tig0015882:-7121 None:intergenic
GTATCTGAGGGTGTAATAAC+TGG 0.502389 tig0015882:-7969 None:intergenic
GCATTGCAGCTGGATTTGTA+GGG 0.503176 tig0015882:-8037 None:intergenic
GATGTTACTGGTGGTGCACC+TGG 0.511282 tig0015882:-8164 None:intergenic
TTCAATGAAAGAACTCAAAA+GGG 0.511868 tig0015882:+7830 Msa1357070:CDS
CTTGAGTTTAACACTGAAGG+TGG 0.514620 tig0015882:-8951 None:intergenic
TCAGCATTGGATTATGCTTG+TGG 0.514748 tig0015882:+8341 Msa1357070:CDS
GCATTTGTGTTTGAAGGAGG+TGG 0.517825 tig0015882:-8254 None:intergenic
CTCTTGATTGTAAGAACCAC+AGG 0.520516 tig0015882:-7693 None:intergenic
CATTTCCACTTCAAGTCTTG+AGG 0.520651 tig0015882:+7490 Msa1357070:CDS
GGAGCATTTGTGTTTGAAGG+AGG 0.521515 tig0015882:-8257 None:intergenic
GAAGACTGGTGCCGGTGTAA+TGG 0.528319 tig0015882:-8114 None:intergenic
GATGTTGTGTTTACAATAGG+AGG 0.530245 tig0015882:-9017 None:intergenic
CAACTCTGTCCCTGAGCAGA+AGG 0.531643 tig0015882:-8278 None:intergenic
TCCTTGCAAAGATGTTCCTG+TGG 0.532621 tig0015882:+7677 Msa1357070:CDS
CCAACAAGTTGCGACTTTGG+AGG 0.533263 tig0015882:+8476 Msa1357070:CDS
GAAGATGTTGGTGGAGCTGT+TGG 0.534788 tig0015882:-8802 None:intergenic
GGAACAGCAGGTGTAGTTGA+AGG 0.536488 tig0015882:-8071 None:intergenic
TTTAGATAAAGGAGGTGAGT+GGG 0.537957 tig0015882:-6843 None:intergenic
GAAAGAACTCAAAAGGGAAG+AGG 0.546037 tig0015882:+7836 Msa1357070:CDS
GGACTATAGATGCAGAAAGA+AGG 0.552427 tig0015882:+9222 Msa1357070:three_prime_UTR
GGAACATTTCCTAATGGAGG+TGG 0.552963 tig0015882:-8212 None:intergenic
GTTGGAGGTAAAGGAACAGC+AGG 0.553488 tig0015882:-8083 None:intergenic
TCTTACAAACTCAAATGTGT+TGG 0.554054 tig0015882:+7224 Msa1357070:CDS
AAAGCATTTCACATGTGCGT+AGG 0.555326 tig0015882:-9656 Msa1357080:three_prime_UTR
TGTAGCTTAATCCAATGCAT+TGG 0.557109 tig0015882:+9537 Msa1357070:three_prime_UTR
GGAGCTGTTGACATTGTTGG+TGG 0.559801 tig0015882:-8769 None:intergenic
ATATATTCATTAAATTATCA+GGG 0.562769 tig0015882:+9580 Msa1357070:three_prime_UTR
GAGGGTGTAATAACTGGTGT+TGG 0.563477 tig0015882:-7963 None:intergenic
ATAATCCAATGCTGATTGAA+GGG 0.564193 tig0015882:-8333 None:intergenic
TTTAACTCGGAAACTGCTGA+AGG 0.564291 tig0015882:-7297 None:intergenic
ACTGTTGTAGGAGTTGTAGC+TGG 0.566759 tig0015882:-8140 None:intergenic
GGTGTTGTTAGATTTGGTGG+TGG 0.572595 tig0015882:-8721 None:intergenic
TTGAGTTTAACACTGAAGGT+GGG 0.573230 tig0015882:-8950 None:intergenic
TCTCTACTTAAACAAAAGCC+AGG 0.575198 tig0015882:+7251 Msa1357070:CDS
TAACTGGTGTTGGATTTGTA+GGG 0.580532 tig0015882:-7953 None:intergenic
CTTCTGCTCAGGGACAGAGT+TGG 0.581714 tig0015882:+8279 Msa1357070:CDS
TTGCTTGAGTTTAACACTGA+AGG 0.581994 tig0015882:-8954 None:intergenic
TACACGTAGGTGTATTCTTG+TGG 0.583103 tig0015882:-9518 None:intergenic
TTAAGAGCATGCATAATCAA+AGG 0.588835 tig0015882:-7408 None:intergenic
CTTTAGATAAAGGAGGTGAG+TGG 0.590303 tig0015882:-6844 None:intergenic
TTTCAGAGCTCGAGGGTGCG+AGG 0.590599 tig0015882:-9042 None:intergenic
TATAATGGTTGGAGTATCTG+AGG 0.591925 tig0015882:-7982 None:intergenic
GAAGGAACTGCTATAATGGT+TGG 0.591976 tig0015882:-7993 None:intergenic
AAGATCTCTCTTCTAGACAA+AGG 0.594620 tig0015882:-7870 None:intergenic
GGTGTTGGAGATGATATCAT+TGG 0.598845 tig0015882:-8670 None:intergenic
ATACTCTCTGATAATGGCCA+AGG 0.605015 tig0015882:+6941 Msa1357070:exon
GTAATGGTTGGAGTTGTTGG+AGG 0.606112 tig0015882:-8098 None:intergenic
ATCATGAGAGAGGAGACACT+TGG 0.607322 tig0015882:+7124 Msa1357070:CDS
GTAAAAGGTTTCAGAGCTCG+AGG 0.607544 tig0015882:-9050 None:intergenic
TGAGTTTAACACTGAAGGTG+GGG 0.608299 tig0015882:-8949 None:intergenic
GAAGTTGAAGTTGATTGTGT+AGG 0.609053 tig0015882:-8694 None:intergenic
TGATATCATTGGTGAAGTTG+AGG 0.609455 tig0015882:-8659 None:intergenic
GTTGAAGTTGATTGTGTAGG+TGG 0.611559 tig0015882:-8691 None:intergenic
CAAATAACTTTAGATAAAGG+AGG 0.616950 tig0015882:-6851 None:intergenic
ATGTTGTGTTTACAATAGGA+GGG 0.618672 tig0015882:-9016 None:intergenic
GTTGTAGCTGGATTGAAGAC+TGG 0.620005 tig0015882:-8128 None:intergenic
ACAAATGCTCCTTCTGCTCA+GGG 0.620260 tig0015882:+8269 Msa1357070:CDS
GGTACTCTATACCCTGTGAC+AGG 0.620454 tig0015882:+9484 Msa1357070:three_prime_UTR
ATTGTGACAGGATTTGTGGA+AGG 0.620979 tig0015882:-8011 None:intergenic
TTAACTCGGAAACTGCTGAA+GGG 0.622484 tig0015882:-7296 None:intergenic
AGAGAGAAGAGAGAAACAAG+AGG 0.623621 tig0015882:-6977 None:intergenic
GCTGGATTGAAGACTGGTGC+CGG 0.624496 tig0015882:-8122 None:intergenic
AACAACTCCAACCATTACAC+CGG 0.624559 tig0015882:+8103 Msa1357070:CDS
GGAGCTGTTGGAATTGATGG+TGG 0.624833 tig0015882:-8790 None:intergenic
ACTGGTGCCGGTGTAATGGT+TGG 0.625957 tig0015882:-8110 None:intergenic
CTGGCATTGTACCTGAAGCT+GGG 0.626526 tig0015882:-8977 None:intergenic
CAGTGATCTTCACATAAGCA+AGG 0.629071 tig0015882:+7449 Msa1357070:CDS
ACCACAGGAACATCTTTGCA+AGG 0.631919 tig0015882:-7678 None:intergenic
AACTCTATAGGTGCATGCTG+AGG 0.632489 tig0015882:-9112 None:intergenic
CATTGCAGCTGGATTTGTAG+GGG 0.635434 tig0015882:-8036 None:intergenic
GAACTCAAAAGGGAAGAGGA+AGG 0.639773 tig0015882:+7840 Msa1357070:CDS
CCAAAAGTGCCTGAAGATGT+TGG 0.642640 tig0015882:-8814 None:intergenic
TGTTGAAGGCAAAAGAAGCA+TGG 0.645483 tig0015882:-8430 None:intergenic
GCTATTGTTGGAATAGATGG+TGG 0.647261 tig0015882:-8745 None:intergenic
ATATAGAAGCACCTGTCACA+GGG 0.656563 tig0015882:-9495 None:intergenic
TCCATTAGGAAATGTTCCAG+TGG 0.658091 tig0015882:+8217 Msa1357070:CDS
ACTGGAACATTTCCTAATGG+AGG 0.658231 tig0015882:-8215 None:intergenic
GAGTAGTGTGATGATCCGAA+TGG 0.661571 tig0015882:-9149 None:intergenic
GCTGATTGAAGGGAAGCTTG+TGG 0.661755 tig0015882:-8323 None:intergenic
TAAAAGGTTTCAGAGCTCGA+GGG 0.668012 tig0015882:-9049 None:intergenic
ATTGTTGGAATAGATGGTGG+AGG 0.673696 tig0015882:-8742 None:intergenic
TCCTATTGTAAACACAACAT+CGG 0.678848 tig0015882:+9019 Msa1357070:CDS
CAGAAGCAGCAAGATTAGCG+CGG 0.682382 tig0015882:-7650 None:intergenic
AAAGTGCCTGAAGATGTTGG+TGG 0.686382 tig0015882:-8811 None:intergenic
ATAATGGTTGGAGTATCTGA+GGG 0.698374 tig0015882:-7981 None:intergenic
TCAGGGACAGAGTTGGTGTG+TGG 0.715688 tig0015882:+8286 Msa1357070:CDS
GGTGGATAAGATGTTACTGG+AGG 0.717889 tig0015882:-8194 None:intergenic
GATTGTTCTCAAATACAACA+GGG 0.719905 tig0015882:+8374 Msa1357070:CDS
GACGACGATGAAGATGATGA+CGG 0.723295 tig0015882:-8631 None:intergenic
CATTGGATTAAGCTACACGT+AGG 0.726032 tig0015882:-9531 None:intergenic
GGTGGATAAGATGTTACTGG+TGG 0.740803 tig0015882:-8173 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATTCATTAAATTATC+AGG + tig0015882:9579-9598 Msa1357070:three_prime_UTR 10.0%
!! ATATATTCATTAAATTATCA+GGG + tig0015882:9580-9599 Msa1357070:three_prime_UTR 10.0%
!! TAGATTACTTTCATTTATAA+AGG + tig0015882:7545-7564 Msa1357070:CDS 15.0%
!! TTAAATGTTAAAATCTTACT+TGG - tig0015882:8527-8546 None:intergenic 15.0%
!!! TATTGATGATCATTTTTAAT+AGG + tig0015882:8589-8608 Msa1357070:intron 15.0%
!! TAGCAAATAACTTTAGATAA+AGG - tig0015882:6857-6876 None:intergenic 20.0%
!!! ATGTTTTTGATGTTTAAATG+TGG - tig0015882:7345-7364 None:intergenic 20.0%
!!! TCTTTTTGCTTCTAAATATT+TGG - tig0015882:7769-7788 None:intergenic 20.0%
!! TTGAAAGAAAGAAAAAACAA+GGG - tig0015882:8554-8573 None:intergenic 20.0%
!! ATTGAAAGAAAGAAAAAACA+AGG - tig0015882:8555-8574 None:intergenic 20.0%
!!! TTAGGTTGTACAATATATTA+TGG + tig0015882:9299-9318 Msa1357070:three_prime_UTR 20.0%
!!! TACATATATTTTGAGAACTT+AGG + tig0015882:9337-9356 Msa1357070:three_prime_UTR 20.0%
! CAAATAACTTTAGATAAAGG+AGG - tig0015882:6854-6873 None:intergenic 25.0%
! ATATTATTTCACTTTCATGC+AGG + tig0015882:7081-7100 Msa1357070:intron 25.0%
!!! AAGTAATGAATTTTTCAACC+TGG - tig0015882:7272-7291 None:intergenic 25.0%
! TTCAATGAAAGAACTCAAAA+GGG + tig0015882:7830-7849 Msa1357070:CDS 25.0%
!! TATCTAGAATATTTCTTCTG+TGG - tig0015882:7905-7924 None:intergenic 25.0%
!!! TGATCATTTTTAATAGGTTC+TGG + tig0015882:8595-8614 Msa1357070:intron 25.0%
!! AGTTTTCAATTGTTTGTTCA+AGG + tig0015882:9361-9380 Msa1357070:three_prime_UTR 25.0%
!! TCATCATTTGACACAATTTT+TGG - tig0015882:9464-9483 None:intergenic 25.0%
! AAAAATTGTGTCAAATGATG+AGG + tig0015882:9463-9482 Msa1357070:three_prime_UTR 25.0%
! AAGGTGTCAAAAAAGAAAGA+GGG - tig0015882:6812-6831 None:intergenic 30.0%
!! AAAGGTGTCAAAAAAGAAAG+AGG - tig0015882:6813-6832 None:intergenic 30.0%
TCAGTGTGATTGATATATAC+AGG - tig0015882:6898-6917 None:intergenic 30.0%
TACACAATACTCTCTGATAA+TGG + tig0015882:6935-6954 Msa1357070:exon 30.0%
TCTCTCTTAATTTCATGTTC+TGG + tig0015882:6993-7012 Msa1357070:CDS 30.0%
TCTTACAAACTCAAATGTGT+TGG + tig0015882:7224-7243 Msa1357070:CDS 30.0%
! TTAAGAGCATGCATAATCAA+AGG - tig0015882:7411-7430 None:intergenic 30.0%
AAGCAAGGAAGTAAAAATCT+CGG + tig0015882:7464-7483 Msa1357070:CDS 30.0%
! ATGATAGAAGATAGCATTGA+TGG + tig0015882:7585-7604 Msa1357070:CDS 30.0%
CTTCAATGAAAGAACTCAAA+AGG + tig0015882:7829-7848 Msa1357070:CDS 30.0%
! ATAATCCAATGCTGATTGAA+GGG - tig0015882:8336-8355 None:intergenic 30.0%
TGATTGTTCTCAAATACAAC+AGG + tig0015882:8373-8392 Msa1357070:CDS 30.0%
GATTGTTCTCAAATACAACA+GGG + tig0015882:8374-8393 Msa1357070:CDS 30.0%
!! CTTTTGATAGTAACTGTTGA+AGG - tig0015882:8447-8466 None:intergenic 30.0%
CATGATTAGTACATGCTTAT+TGG - tig0015882:8891-8910 None:intergenic 30.0%
CAATAAGCATGTACTAATCA+TGG + tig0015882:8889-8908 Msa1357070:intron 30.0%
ATGTTGTGTTTACAATAGGA+GGG - tig0015882:9019-9038 None:intergenic 30.0%
TCCTATTGTAAACACAACAT+CGG + tig0015882:9019-9038 Msa1357070:CDS 30.0%
AAAATATTCTAAGCAGCTCA+TGG - tig0015882:9281-9300 None:intergenic 30.0%
! TGAGCTGCTTAGAATATTTT+AGG + tig0015882:9281-9300 Msa1357070:three_prime_UTR 30.0%
CAATGTAGTTCAGTGTAAAT+TGG + tig0015882:9396-9415 Msa1357070:three_prime_UTR 30.0%
GTCAGAGTTGTTATAATTTG+AGG + tig0015882:9418-9437 Msa1357070:three_prime_UTR 30.0%
TTTAGATAAAGGAGGTGAGT+GGG - tig0015882:6846-6865 None:intergenic 35.0%
TCTCTACTTAAACAAAAGCC+AGG + tig0015882:7251-7270 Msa1357070:CDS 35.0%
!! CACTAGTTGAGCTTTTAACT+CGG - tig0015882:7313-7332 None:intergenic 35.0%
AAAAACATCATTGCTAGCTG+TGG + tig0015882:7357-7376 Msa1357070:CDS 35.0%
CGAGTTACTTCTATTGAACT+TGG - tig0015882:7729-7748 None:intergenic 35.0%
AAGATCTCTCTTCTAGACAA+AGG - tig0015882:7873-7892 None:intergenic 35.0%
! TAACTGGTGTTGGATTTGTA+GGG - tig0015882:7956-7975 None:intergenic 35.0%
! ATAACTGGTGTTGGATTTGT+AGG - tig0015882:7957-7976 None:intergenic 35.0%
! ATAATGGTTGGAGTATCTGA+GGG - tig0015882:7984-8003 None:intergenic 35.0%
! TATAATGGTTGGAGTATCTG+AGG - tig0015882:7985-8004 None:intergenic 35.0%
! CATAATCCAATGCTGATTGA+AGG - tig0015882:8337-8356 None:intergenic 35.0%
TTGGATTATGCTTGTGGAAA+TGG + tig0015882:8347-8366 Msa1357070:CDS 35.0%
TGATATCATTGGTGAAGTTG+AGG - tig0015882:8662-8681 None:intergenic 35.0%
! GAAGTTGAAGTTGATTGTGT+AGG - tig0015882:8697-8716 None:intergenic 35.0%
! AATGCTGTCTTTTGTAGCTA+TGG + tig0015882:8924-8943 Msa1357070:intron 35.0%
TTGAGTTTAACACTGAAGGT+GGG - tig0015882:8953-8972 None:intergenic 35.0%
TTGCTTGAGTTTAACACTGA+AGG - tig0015882:8957-8976 None:intergenic 35.0%
GATGTTGTGTTTACAATAGG+AGG - tig0015882:9020-9039 None:intergenic 35.0%
AAGACTATACAGCCAGTAAA+AGG - tig0015882:9068-9087 None:intergenic 35.0%
!! AACCTGTTTCTGAACTCTAT+AGG - tig0015882:9127-9146 None:intergenic 35.0%
! TCAGAAACAGGTTTTCCATT+CGG + tig0015882:9134-9153 Msa1357070:three_prime_UTR 35.0%
TGTAGCTTAATCCAATGCAT+TGG + tig0015882:9537-9556 Msa1357070:three_prime_UTR 35.0%
! GGTTAAAAAGTCAAGGTGTT+TGG - tig0015882:9638-9657 Msa1357080:three_prime_UTR 35.0%
CTTTAGATAAAGGAGGTGAG+TGG - tig0015882:6847-6866 None:intergenic 40.0%
!! ATACTCTCTGATAATGGCCA+AGG + tig0015882:6941-6960 Msa1357070:exon 40.0%
AGAGAGAAGAGAGAAACAAG+AGG - tig0015882:6980-6999 None:intergenic 40.0%
TCACTTTCATGCAGGTTCTT+TGG + tig0015882:7089-7108 Msa1357070:intron 40.0%
TTTCATGCAGGTTCTTTGGT+GGG + tig0015882:7093-7112 Msa1357070:intron 40.0%
! GGTTTTTCCTATCATGAGAG+AGG + tig0015882:7114-7133 Msa1357070:CDS 40.0%
ATGAAGAAACTTTGCTCTGC+TGG - tig0015882:7161-7180 None:intergenic 40.0%
TTAACTCGGAAACTGCTGAA+GGG - tig0015882:7299-7318 None:intergenic 40.0%
TTTAACTCGGAAACTGCTGA+AGG - tig0015882:7300-7319 None:intergenic 40.0%
CAGTGATCTTCACATAAGCA+AGG + tig0015882:7449-7468 Msa1357070:CDS 40.0%
CATTTCCACTTCAAGTCTTG+AGG + tig0015882:7490-7509 Msa1357070:CDS 40.0%
AACTTCCTCAAGACTTGAAG+TGG - tig0015882:7498-7517 None:intergenic 40.0%
CTCTTGATTGTAAGAACCAC+AGG - tig0015882:7696-7715 None:intergenic 40.0%
GAAAGAACTCAAAAGGGAAG+AGG + tig0015882:7836-7855 Msa1357070:CDS 40.0%
GTAGGGTAAACTGTGTTTGT+TGG - tig0015882:7939-7958 None:intergenic 40.0%
! GTATCTGAGGGTGTAATAAC+TGG - tig0015882:7972-7991 None:intergenic 40.0%
GAAGGAACTGCTATAATGGT+TGG - tig0015882:7996-8015 None:intergenic 40.0%
TGTGGAAGGAACTGCTATAA+TGG - tig0015882:8000-8019 None:intergenic 40.0%
ATTGTGACAGGATTTGTGGA+AGG - tig0015882:8014-8033 None:intergenic 40.0%
GGAAATTGTGACAGGATTTG+TGG - tig0015882:8018-8037 None:intergenic 40.0%
TTTGTAGGGGAAATTGTGAC+AGG - tig0015882:8026-8045 None:intergenic 40.0%
GTAGTTGAAGGAACTGTTAC+TGG - tig0015882:8062-8081 None:intergenic 40.0%
AACAACTCCAACCATTACAC+CGG + tig0015882:8103-8122 Msa1357070:CDS 40.0%
ACATCTTATCCACCTCCATT+AGG + tig0015882:8203-8222 Msa1357070:CDS 40.0%
ACTGGAACATTTCCTAATGG+AGG - tig0015882:8218-8237 None:intergenic 40.0%
TCCATTAGGAAATGTTCCAG+TGG + tig0015882:8217-8236 Msa1357070:CDS 40.0%
ACCACTGGAACATTTCCTAA+TGG - tig0015882:8221-8240 None:intergenic 40.0%
!! GAAGGAGCATTTGTGTTTGA+AGG - tig0015882:8263-8282 None:intergenic 40.0%
! TCAGCATTGGATTATGCTTG+TGG + tig0015882:8341-8360 Msa1357070:CDS 40.0%
TGTTGAAGGCAAAAGAAGCA+TGG - tig0015882:8433-8452 None:intergenic 40.0%
! GGTGTTGGAGATGATATCAT+TGG - tig0015882:8673-8692 None:intergenic 40.0%
! GTTGAAGTTGATTGTGTAGG+TGG - tig0015882:8694-8713 None:intergenic 40.0%
! ATTGTTGGAATAGATGGTGG+AGG - tig0015882:8745-8764 None:intergenic 40.0%
GCTATTGTTGGAATAGATGG+TGG - tig0015882:8748-8767 None:intergenic 40.0%
GGAGCTATTGTTGGAATAGA+TGG - tig0015882:8751-8770 None:intergenic 40.0%
! ATTGTTGGTGGAGCTATTGT+TGG - tig0015882:8760-8779 None:intergenic 40.0%
! CAACATCTTCAGGCACTTTT+GGG + tig0015882:8815-8834 Msa1357070:CDS 40.0%
TGAGTTTAACACTGAAGGTG+GGG - tig0015882:8952-8971 None:intergenic 40.0%
CTTGAGTTTAACACTGAAGG+TGG - tig0015882:8954-8973 None:intergenic 40.0%
! TCAGGTACAATGCCAGATTT+TGG + tig0015882:8984-9003 Msa1357070:CDS 40.0%
! CAGGTACAATGCCAGATTTT+GGG + tig0015882:8985-9004 Msa1357070:CDS 40.0%
GCCGATGTTGTGTTTACAAT+AGG - tig0015882:9023-9042 None:intergenic 40.0%
TAAAAGGTTTCAGAGCTCGA+GGG - tig0015882:9052-9071 None:intergenic 40.0%
CACCTATAGAGTTCAGAAAC+AGG + tig0015882:9122-9141 Msa1357070:exon 40.0%
CATTACATGCTGCAGTGTTA+AGG + tig0015882:9201-9220 Msa1357070:three_prime_UTR 40.0%
GGACTATAGATGCAGAAAGA+AGG + tig0015882:9222-9241 Msa1357070:three_prime_UTR 40.0%
!! ATATAGAAGCACCTGTCACA+GGG - tig0015882:9498-9517 None:intergenic 40.0%
TACACGTAGGTGTATTCTTG+TGG - tig0015882:9521-9540 None:intergenic 40.0%
CATTGGATTAAGCTACACGT+AGG - tig0015882:9534-9553 None:intergenic 40.0%
AGAATCAGCATCCAATGCAT+TGG - tig0015882:9551-9570 None:intergenic 40.0%
!! GAAAAACCAGAAAGCACTGA+TGG + tig0015882:9602-9621 Msa1357070:three_prime_UTR 40.0%
GTGCGTAGGTTAAAAAGTCA+AGG - tig0015882:9645-9664 Msa1357080:three_prime_UTR 40.0%
! AAAGCATTTCACATGTGCGT+AGG - tig0015882:9659-9678 Msa1357080:three_prime_UTR 40.0%
GCACATGTGAAATGCTTTCA+AGG + tig0015882:9661-9680 Msa1357070:three_prime_UTR 40.0%
TGAGTGGGAAGGAGAGAAAA+AGG - tig0015882:6831-6850 None:intergenic 45.0%
CTTTCATGCAGGTTCTTTGG+TGG + tig0015882:7092-7111 Msa1357070:intron 45.0%
AGTGTCTCCTCTCTCATGAT+AGG - tig0015882:7124-7143 None:intergenic 45.0%
ATCATGAGAGAGGAGACACT+TGG + tig0015882:7124-7143 Msa1357070:CDS 45.0%
GAGCTTTCGTCACAGATCAT+AGG + tig0015882:7190-7209 Msa1357070:CDS 45.0%
TCCTTGCAAAGATGTTCCTG+TGG + tig0015882:7677-7696 Msa1357070:CDS 45.0%
ACCACAGGAACATCTTTGCA+AGG - tig0015882:7681-7700 None:intergenic 45.0%
GAACTCAAAAGGGAAGAGGA+AGG + tig0015882:7840-7859 Msa1357070:CDS 45.0%
! GAGGGTGTAATAACTGGTGT+TGG - tig0015882:7966-7985 None:intergenic 45.0%
CATTGCAGCTGGATTTGTAG+GGG - tig0015882:8039-8058 None:intergenic 45.0%
GCATTGCAGCTGGATTTGTA+GGG - tig0015882:8040-8059 None:intergenic 45.0%
GTTGGAGTTGTTGGAGGTAA+AGG - tig0015882:8095-8114 None:intergenic 45.0%
GTAATGGTTGGAGTTGTTGG+AGG - tig0015882:8101-8120 None:intergenic 45.0%
GGTGTAATGGTTGGAGTTGT+TGG - tig0015882:8104-8123 None:intergenic 45.0%
GTTGTAGCTGGATTGAAGAC+TGG - tig0015882:8131-8150 None:intergenic 45.0%
ACTGTTGTAGGAGTTGTAGC+TGG - tig0015882:8143-8162 None:intergenic 45.0%
ACAACTCCTACAACAGTTCC+AGG + tig0015882:8146-8165 Msa1357070:CDS 45.0%
GGTGGATAAGATGTTACTGG+TGG - tig0015882:8176-8195 None:intergenic 45.0%
GGAGGTGGATAAGATGTTAC+TGG - tig0015882:8179-8198 None:intergenic 45.0%
GGATAAGATGTTACTGGAGG+TGG - tig0015882:8194-8213 None:intergenic 45.0%
GGTGGATAAGATGTTACTGG+AGG - tig0015882:8176-8195 None:intergenic 45.0%
GGAGGTGGATAAGATGTTAC+TGG - tig0015882:8179-8198 None:intergenic 45.0%
GGAACATTTCCTAATGGAGG+TGG - tig0015882:8215-8234 None:intergenic 45.0%
GCATTTGTGTTTGAAGGAGG+TGG - tig0015882:8257-8276 None:intergenic 45.0%
! GGAGCATTTGTGTTTGAAGG+AGG - tig0015882:8260-8279 None:intergenic 45.0%
ACAAATGCTCCTTCTGCTCA+GGG + tig0015882:8269-8288 Msa1357070:CDS 45.0%
AGCTTCCCTTCAATCAGCAT+TGG + tig0015882:8328-8347 Msa1357070:CDS 45.0%
! GCATGGTTCTGCAAAGTAAC+AGG - tig0015882:8416-8435 None:intergenic 45.0%
GACGACGATGAAGATGATGA+CGG - tig0015882:8634-8653 None:intergenic 45.0%
GTTGATTGTGTAGGTGGTGT+TGG - tig0015882:8688-8707 None:intergenic 45.0%
!! GGTGTTGTTAGATTTGGTGG+TGG - tig0015882:8724-8743 None:intergenic 45.0%
!! GGAGGTGTTGTTAGATTTGG+TGG - tig0015882:8727-8746 None:intergenic 45.0%
!! GGTGGAGGTGTTGTTAGATT+TGG - tig0015882:8730-8749 None:intergenic 45.0%
! AAAGTGCCTGAAGATGTTGG+TGG - tig0015882:8814-8833 None:intergenic 45.0%
!! CCAAAAGTGCCTGAAGATGT+TGG - tig0015882:8817-8836 None:intergenic 45.0%
! CCAACATCTTCAGGCACTTT+TGG + tig0015882:8814-8833 Msa1357070:CDS 45.0%
GTAAAAGGTTTCAGAGCTCG+AGG - tig0015882:9053-9072 None:intergenic 45.0%
!!! CGAGCTCTGAAACCTTTTAC+TGG + tig0015882:9053-9072 Msa1357070:CDS 45.0%
AACTCTATAGGTGCATGCTG+AGG - tig0015882:9115-9134 None:intergenic 45.0%
GAGTAGTGTGATGATCCGAA+TGG - tig0015882:9152-9171 None:intergenic 45.0%
!! GATATAGAAGCACCTGTCAC+AGG - tig0015882:9499-9518 None:intergenic 45.0%
GATAAAGGAGGTGAGTGGGA+AGG - tig0015882:6842-6861 None:intergenic 50.0%
GAGGCAGAAAGAAGCTTCCT+TGG - tig0015882:6961-6980 None:intergenic 50.0%
CAGAAGCAGCAAGATTAGCG+CGG - tig0015882:7653-7672 None:intergenic 50.0%
GGCATTGCAGCTGGATTTGT+AGG - tig0015882:8041-8060 None:intergenic 50.0%
ACTGTTACTGGCATTGCAGC+TGG - tig0015882:8050-8069 None:intergenic 50.0%
GGAACAGCAGGTGTAGTTGA+AGG - tig0015882:8074-8093 None:intergenic 50.0%
GTTGGAGGTAAAGGAACAGC+AGG - tig0015882:8086-8105 None:intergenic 50.0%
TTGAAGGAGGTGGCTGTTGA+GGG - tig0015882:8247-8266 None:intergenic 50.0%
TTTGAAGGAGGTGGCTGTTG+AGG - tig0015882:8248-8267 None:intergenic 50.0%
CACAAATGCTCCTTCTGCTC+AGG + tig0015882:8268-8287 Msa1357070:CDS 50.0%
! AGTTGGTGTGTGGCGAAAAC+AGG + tig0015882:8296-8315 Msa1357070:CDS 50.0%
GCTGATTGAAGGGAAGCTTG+TGG - tig0015882:8326-8345 None:intergenic 50.0%
!! AAGTCGCAACTTGTTGGAGC+TGG - tig0015882:8473-8492 None:intergenic 50.0%
GCTCCAACAAGTTGCGACTT+TGG + tig0015882:8473-8492 Msa1357070:CDS 50.0%
CCTCCAAAGTCGCAACTTGT+TGG - tig0015882:8479-8498 None:intergenic 50.0%
CCAACAAGTTGCGACTTTGG+AGG + tig0015882:8476-8495 Msa1357070:CDS 50.0%
GGAGCTGTTGACATTGTTGG+TGG - tig0015882:8772-8791 None:intergenic 50.0%
GGTGGAGCTGTTGACATTGT+TGG - tig0015882:8775-8794 None:intergenic 50.0%
GGAGCTGTTGGAATTGATGG+TGG - tig0015882:8793-8812 None:intergenic 50.0%
GGTGGAGCTGTTGGAATTGA+TGG - tig0015882:8796-8815 None:intergenic 50.0%
! GAAGATGTTGGTGGAGCTGT+TGG - tig0015882:8805-8824 None:intergenic 50.0%
ACAGCTCCACCAACATCTTC+AGG + tig0015882:8805-8824 Msa1357070:CDS 50.0%
!! GGCACTTTTGGGTAAGTGAG+AGG + tig0015882:8826-8845 Msa1357070:intron 50.0%
AACTCAAGCAACCCAGCTTC+AGG + tig0015882:8966-8985 Msa1357070:CDS 50.0%
CTGGCATTGTACCTGAAGCT+GGG - tig0015882:8980-8999 None:intergenic 50.0%
TCTGGCATTGTACCTGAAGC+TGG - tig0015882:8981-9000 None:intergenic 50.0%
GGGCTATCAGACCCAAAATC+TGG - tig0015882:8999-9018 None:intergenic 50.0%
CTGAGGCTTGCAGTGACAAA+TGG - tig0015882:9098-9117 None:intergenic 50.0%
GGTACTCTATACCCTGTGAC+AGG + tig0015882:9484-9503 Msa1357070:three_prime_UTR 50.0%
TGATCGCCATCAGTGCTTTC+TGG - tig0015882:9611-9630 Msa1357080:three_prime_UTR 50.0%
!! ACTGGTGCCGGTGTAATGGT+TGG - tig0015882:8113-8132 None:intergenic 55.0%
!! GAAGACTGGTGCCGGTGTAA+TGG - tig0015882:8117-8136 None:intergenic 55.0%
GCTGGATTGAAGACTGGTGC+CGG - tig0015882:8125-8144 None:intergenic 55.0%
GGTGCACCTGGAACTGTTGT+AGG - tig0015882:8155-8174 None:intergenic 55.0%
! GATGTTACTGGTGGTGCACC+TGG - tig0015882:8167-8186 None:intergenic 55.0%
GGCTGTTGAGGGTTTACCAC+TGG - tig0015882:8236-8255 None:intergenic 55.0%
CAACTCTGTCCCTGAGCAGA+AGG - tig0015882:8281-8300 None:intergenic 55.0%
CTTCTGCTCAGGGACAGAGT+TGG + tig0015882:8279-8298 Msa1357070:CDS 55.0%
!! TCAGGGACAGAGTTGGTGTG+TGG + tig0015882:8286-8305 Msa1357070:CDS 55.0%
!! GGAGGCGCTGCTATGTTAGT+TGG + tig0015882:8494-8513 Msa1357070:CDS 55.0%
! TTTCAGAGCTCGAGGGTGCG+AGG - tig0015882:9045-9064 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0015882 gene 6804 9700 6804 ID=Msa1357070;Name=Msa1357070
tig0015882 mRNA 6804 9700 6804 ID=Msa1357070-mRNA-1;Parent=Msa1357070;Name=Msa1357070-mRNA-1;_AED=0.02;_eAED=0.02;_QI=150|1|1|1|0.33|0.25|4|570|633
tig0015882 exon 6804 7014 6804 ID=Msa1357070-mRNA-1:exon:10229;Parent=Msa1357070-mRNA-1
tig0015882 exon 7103 8527 7103 ID=Msa1357070-mRNA-1:exon:10230;Parent=Msa1357070-mRNA-1
tig0015882 exon 8611 8836 8611 ID=Msa1357070-mRNA-1:exon:10231;Parent=Msa1357070-mRNA-1
tig0015882 exon 8941 9700 8941 ID=Msa1357070-mRNA-1:exon:10232;Parent=Msa1357070-mRNA-1
tig0015882 five_prime_UTR 6804 6953 6804 ID=Msa1357070-mRNA-1:five_prime_utr;Parent=Msa1357070-mRNA-1
tig0015882 CDS 6954 7014 6954 ID=Msa1357070-mRNA-1:cds;Parent=Msa1357070-mRNA-1
tig0015882 CDS 7103 8527 7103 ID=Msa1357070-mRNA-1:cds;Parent=Msa1357070-mRNA-1
tig0015882 CDS 8611 8836 8611 ID=Msa1357070-mRNA-1:cds;Parent=Msa1357070-mRNA-1
tig0015882 CDS 8941 9130 8941 ID=Msa1357070-mRNA-1:cds;Parent=Msa1357070-mRNA-1
tig0015882 three_prime_UTR 9131 9700 9131 ID=Msa1357070-mRNA-1:three_prime_utr;Parent=Msa1357070-mRNA-1
Gene Sequence

>Msa1357070

ATGGCCAAGGAAGCTTCTTTCTGCCTCTTGTTTCTCTCTTCTCTCTTAATTTCATGTTCTGGTTCTTTGGTGGGTTTTTCCTATCATGAGAGAGGAGACACTTGGACATCATTACTCCAGCAGAGCAAAGTTTCTTCATCTCAGATTCGAGCTTTCGTCACAGATCATAGGATTTTGAGCACTCTTACAAACTCAAATGTGTTGGTTGATCTCTACTTAAACAAAAGCCAGGTTGAAAAATTCATTACTTCAAACCCTTCAGCAGTTTCCGAGTTAAAAGCTCAACTAGTGAGCTTTCTTCCACATTTAAACATCAAAAACATCATTGCTAGCTGTGGAAGTGAGTGCTTAGTACAAAATGAGATACCTTTGATTATGCATGCTCTTAAATCAATTCATTCAATTCTCAGTGATCTTCACATAAGCAAGGAAGTAAAAATCTCGGTCGCATTTCCACTTCAAGTCTTGAGGAAGTTGAATGCATCACAAGAACACGAAATTCGTAGATTACTTTCATTTATAAAGGAAACGAAATCGTTTGTAATGATAGAAGATAGCATTGATGGAGAATTAAGTGTGAATGATCATTTTGTTCAAACAATTATCAACCGCGCTAATCTTGCTGCTTCTGTTCTTCCTTGCAAAGATGTTCCTGTGGTTCTTACAATCAAGAGTTCAGTTATACCAAGTTCAATAGAAGTAACTCGATTTAGTAAAAGAGTATCCAAATATTTAGAAGCAAAAAGAATAGCTGCATTATATGTAGAACTGCATACAACAGAAGATTCTTCAATGAAAGAACTCAAAAGGGAAGAGGAAGGTATGTTTCCTTTGTCTAGAAGAGAGATCTTAAGCAAATTCCACAGAAGAAATATTCTAGATAACACAAACAGTCCAACAAACACAGTTTACCCTACAAATCCAACACCAGTTATTACACCCTCAGATACTCCAACCATTATAGCAGTTCCTTCCACAAATCCTGTCACAATTTCCCCTACAAATCCAGCTGCAATGCCAGTAACAGTTCCTTCAACTACACCTGCTGTTCCTTTACCTCCAACAACTCCAACCATTACACCGGCACCAGTCTTCAATCCAGCTACAACTCCTACAACAGTTCCAGGTGCACCACCAGTAACATCTTATCCACCTCCAGTAACATCTTATCCACCTCCATTAGGAAATGTTCCAGTGGTAAACCCTCAACAGCCACCTCCTTCAAACACAAATGCTCCTTCTGCTCAGGGACAGAGTTGGTGTGTGGCGAAAACAGGTGCTCCACAAGCTTCCCTTCAATCAGCATTGGATTATGCTTGTGGAAATGGTGCTGATTGTTCTCAAATACAACAGGGTGCAAGTTGTTACAGTCCTGTTACTTTGCAGAACCATGCTTCTTTTGCCTTCAACAGTTACTATCAAAAGAAGCCAGCTCCAACAAGTTGCGACTTTGGAGGCGCTGCTATGTTAGTTGGCTCAAATCCAAGTTCTGGTTCTTGCATTTATCCGTCATCATCTTCATCGTCGTCGTCATCCTCAACTTCACCAATGATATCATCTCCAACACCACCTACACAATCAACTTCAACTTCAATACCACCACCAAATCTAACAACACCTCCACCATCTATTCCAACAATAGCTCCACCAACAATGTCAACAGCTCCACCATCAATTCCAACAGCTCCACCAACATCTTCAGGCACTTTTGGCTATGGTACCCCACCTTCAGTGTTAAACTCAAGCAACCCAGCTTCAGGTACAATGCCAGATTTTGGGTCTGATAGCCCTCCTATTGTAAACACAACATCGGCCTCGCACCCTCGAGCTCTGAAACCTTTTACTGGCTGTATAGTCTTGATGATTCCATTTGTCACTGCAAGCCTCAGCATGCACCTATAG
Protein sequence

>Msa1357070

MAKEASFCLLFLSSLLISCSGSLVGFSYHERGDTWTSLLQQSKVSSSQIRAFVTDHRILSTLTNSNVLVDLYLNKSQVEKFITSNPSAVSELKAQLVSFLPHLNIKNIIASCGSECLVQNEIPLIMHALKSIHSILSDLHISKEVKISVAFPLQVLRKLNASQEHEIRRLLSFIKETKSFVMIEDSIDGELSVNDHFVQTIINRANLAASVLPCKDVPVVLTIKSSVIPSSIEVTRFSKRVSKYLEAKRIAALYVELHTTEDSSMKELKREEEGMFPLSRREILSKFHRRNILDNTNSPTNTVYPTNPTPVITPSDTPTIIAVPSTNPVTISPTNPAAMPVTVPSTTPAVPLPPTTPTITPAPVFNPATTPTTVPGAPPVTSYPPPVTSYPPPLGNVPVVNPQQPPPSNTNAPSAQGQSWCVAKTGAPQASLQSALDYACGNGADCSQIQQGASCYSPVTLQNHASFAFNSYYQKKPAPTSCDFGGAAMLVGSNPSSGSCIYPSSSSSSSSSSTSPMISSPTPPTQSTSTSIPPPNLTTPPPSIPTIAPPTMSTAPPSIPTAPPTSSGTFGYGTPPSVLNSSNPASGTMPDFGSDSPPIVNTTSASHPRALKPFTGCIVLMIPFVTASLSMHL*