AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0711130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0711130 MtrunA17_Chr5g0393831 96.637 446 12 2 1 445 1 444 0.0 874
Msa0711130 MtrunA17_Chr1g0206981 55.196 433 161 8 26 445 12 424 2.54e-148 429
Msa0711130 MtrunA17_Chr8g0384941 44.783 460 169 7 17 443 15 422 6.19e-120 356
Msa0711130 MtrunA17_Chr2g0333971 45.796 452 201 7 8 444 24 446 5.15e-116 347
Msa0711130 MtrunA17_Chr5g0419611 40.000 435 207 9 14 444 19 403 2.78e-95 292
Msa0711130 MtrunA17_Chr8g0380621 40.230 435 187 10 23 444 46 420 3.00e-94 290
Msa0711130 MtrunA17_Chr8g0385861 54.419 215 70 5 27 213 24 238 1.12e-67 216
Msa0711130 MtrunA17_Chr1g0165841 48.259 201 98 2 36 235 20 215 3.56e-55 187
Msa0711130 MtrunA17_Chr1g0165841 43.357 143 77 2 302 444 227 365 3.34e-21 94.7
Msa0711130 MtrunA17_Chr1g0165861 38.023 263 104 4 176 436 2 207 1.48e-48 165
Msa0711130 MtrunA17_Chr1g0176551 44.712 208 46 6 36 243 10 148 1.34e-38 140
Msa0711130 MtrunA17_Chr1g0176551 35.398 113 64 2 333 445 165 268 8.30e-11 62.4
Msa0711130 MtrunA17_Chr8g0385851 54.867 113 48 1 332 444 1 110 3.74e-36 129
Msa0711130 MtrunA17_Chr1g0187901 48.980 147 68 3 301 444 246 388 1.50e-34 132
Msa0711130 MtrunA17_Chr2g0330321 57.554 139 27 6 56 190 1 111 4.23e-34 124
Msa0711130 MtrunA17_Chr7g0260731 57.000 100 38 1 302 401 155 249 1.54e-29 116
Msa0711130 MtrunA17_Chr1g0165871 48.113 106 48 2 26 124 5 110 3.74e-24 98.2
Msa0711130 MtrunA17_Chr2g0307921 55.000 80 36 0 26 105 8 87 5.63e-23 93.2
Msa0711130 MtrunA17_Chr2g0307931 62.121 66 25 0 40 105 20 85 3.32e-20 85.1
Msa0711130 MtrunA17_Chr1g0187911 54.651 86 26 2 319 401 62 137 4.23e-20 86.7
Msa0711130 MtrunA17_Chr7g0260721 55.714 70 30 1 23 91 12 81 4.85e-19 81.6
Msa0711130 MtrunA17_Chr2g0320641 23.301 412 219 13 40 444 123 444 3.15e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0711130 AT3G19640.1 64.000 450 110 7 36 445 47 484 0.0 527
Msa0711130 AT1G80900.2 45.077 457 203 8 1 444 19 440 1.92e-114 343
Msa0711130 AT1G80900.1 45.077 457 203 8 1 444 19 440 1.92e-114 343
Msa0711130 AT1G16010.3 46.591 440 178 10 23 444 40 440 5.33e-114 342
Msa0711130 AT1G16010.1 46.591 440 178 10 23 444 40 440 5.33e-114 342
Msa0711130 AT1G16010.2 46.591 440 178 10 23 444 40 440 5.33e-114 342
Msa0711130 AT5G09690.1 43.721 430 181 6 19 444 12 384 8.99e-104 314
Msa0711130 AT5G09690.2 43.721 430 181 6 19 444 23 395 1.27e-103 314
Msa0711130 AT5G09690.12 46.253 387 150 5 19 401 23 355 3.95e-100 305
Msa0711130 AT3G58970.1 41.055 436 202 7 17 444 46 434 1.31e-99 305
Msa0711130 AT2G03620.1 40.952 420 208 8 25 444 40 419 1.18e-93 289
Msa0711130 AT2G03620.2 40.952 420 208 8 25 444 40 419 1.18e-93 289
Msa0711130 AT5G09690.7 45.355 366 136 5 19 377 12 320 1.72e-89 275
Msa0711130 AT5G09690.9 45.961 359 140 4 19 375 12 318 3.63e-89 274
Msa0711130 AT5G09690.3 45.961 359 140 4 19 375 12 318 4.08e-89 274
Msa0711130 AT5G09690.8 45.152 361 144 4 19 377 23 331 1.12e-85 266
Msa0711130 AT5G09690.11 45.152 361 144 4 19 377 23 331 1.12e-85 266
Msa0711130 AT5G09690.13 45.378 357 145 3 19 375 23 329 2.24e-85 265
Msa0711130 AT5G09690.4 38.785 428 190 8 19 444 12 369 1.74e-73 235
Msa0711130 AT4G28580.1 33.487 433 204 11 27 445 46 408 9.05e-67 219
Msa0711130 AT5G09690.14 53.881 219 93 2 19 235 23 235 1.08e-66 215
Msa0711130 AT5G09690.10 53.881 219 93 2 19 235 23 235 1.08e-66 215
Msa0711130 AT5G09690.6 53.881 219 93 2 19 235 23 235 1.08e-66 215
Msa0711130 AT5G09690.5 53.917 217 96 1 19 235 12 224 1.19e-66 215
Msa0711130 AT5G09720.1 48.837 215 102 2 23 235 8 216 1.19e-57 192
Msa0711130 AT5G09720.2 48.837 215 102 2 23 235 8 216 1.19e-57 192
Msa0711130 AT5G64560.1 47.794 136 67 2 309 444 261 392 8.04e-32 125
Msa0711130 AT5G64560.2 50.806 124 44 3 309 432 261 367 3.50e-29 118
Msa0711130 AT5G22830.1 29.016 193 118 4 39 227 135 312 2.67e-11 65.9
Msa0711130 AT5G22830.2 29.016 193 118 4 39 227 135 312 2.67e-11 65.9

Find 128 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGGAGGGTTGTAAAATT+TGG 0.093469 5_1:-2980823 None:intergenic
TGTTTGGTGCTTCGAATTTA+AGG 0.165870 5_1:-2979826 Msa0711130:CDS
AGCCGTTTGGTTGCGATTAC+TGG 0.222757 5_1:-2980124 Msa0711130:CDS
GAAGATTAGTACTCTCAATT+TGG 0.225739 5_1:-2980167 Msa0711130:CDS
ACTGTTGGCGGCGGCACTGC+TGG 0.245438 5_1:-2978479 Msa0711130:CDS
ATTGGAGGGTTGTAAAATTT+GGG 0.275816 5_1:-2980822 Msa0711130:five_prime_UTR
TGAAGCAGCTTGTAGTGTTT+TGG 0.284919 5_1:-2980248 Msa0711130:CDS
ATCGCTACCGTTGGCGTTAC+CGG 0.300032 5_1:+2980708 None:intergenic
ATATCGTTGGAAGCGGGTTT+AGG 0.335773 5_1:-2979908 Msa0711130:CDS
GTGCAAGCACAAACGCTTGC+TGG 0.343936 5_1:-2978423 Msa0711130:CDS
GTACCTTTCTTCCGAATTGC+CGG 0.346470 5_1:+2980655 None:intergenic
TCCTACCCTTCCACCGTTCT+CGG 0.346952 5_1:-2980505 Msa0711130:CDS
AGTGCCTTCGTTGTTGTTGC+TGG 0.353794 5_1:-2978572 Msa0711130:CDS
CTGTTGGCGGCGGCACTGCT+GGG 0.366005 5_1:-2978478 Msa0711130:CDS
TGAGAATCTTCCTGATTATC+AGG 0.379240 5_1:+2980322 None:intergenic
TACTCCTCGTCCTGATAATC+AGG 0.381579 5_1:-2980332 Msa0711130:CDS
GTTTGGTGCTTCGAATTTAA+GGG 0.389449 5_1:-2979825 Msa0711130:CDS
CTGTTTGATGATAAATTGTA+TGG 0.401637 5_1:-2978521 Msa0711130:CDS
CAAGCGTTTGTGCTTGCACC+AGG 0.405920 5_1:+2978427 None:intergenic
TGCTCTTTCTCTTCCGAGAA+CGG 0.416716 5_1:+2980492 None:intergenic
CTCGATCACCTGTGCTTCGC+CGG 0.418290 5_1:+2980600 None:intergenic
TGCACGTGACCTTCGAATCC+TGG 0.419970 5_1:-2980539 Msa0711130:CDS
GATGTTGATGTTAATCAAAC+TGG 0.433420 5_1:-2979944 Msa0711130:CDS
GAAGTCACTTCAACAGCAAT+TGG 0.436400 5_1:-2980023 Msa0711130:CDS
AGCAACCACATACAAGAATA+TGG 0.438257 5_1:+2978454 None:intergenic
ACAGAACCATCTTCTCCAAA+TGG 0.439397 5_1:-2978627 Msa0711130:CDS
CCGCCGCCGTACCGGCAATT+CGG 0.439847 5_1:-2980666 Msa0711130:CDS
CTCCTCAACATCAAGCTTAG+TGG 0.445396 5_1:+2979760 None:intergenic
TGTATGTGGTTGCTATTGCC+TGG 0.445914 5_1:-2978445 Msa0711130:CDS
AATTCTAGAGAGCAAATGTT+TGG 0.448954 5_1:-2979842 Msa0711130:CDS
TGACTCCCATTTGGAGAAGA+TGG 0.450914 5_1:+2978621 None:intergenic
GCTTGATGATGATGAAGATA+TGG 0.464583 5_1:-2980065 Msa0711130:CDS
AACAGCTGCCGCCGCCGTAC+CGG 0.467572 5_1:-2980674 Msa0711130:CDS
TGAGGAGCTTGAGATGCTCT+TGG 0.467583 5_1:-2979744 Msa0711130:CDS
AGGAGGAGAGGTGAATTATG+AGG 0.467721 5_1:-2979882 Msa0711130:CDS
TCCGTTTGAATTCGTTGCGC+TGG 0.471296 5_1:-2980281 Msa0711130:CDS
CAAACGCTTGCTGGAGTGAA+TGG 0.472005 5_1:-2978414 Msa0711130:exon
CATGTTGACCACGGCAACTC+TGG 0.474836 5_1:-2978600 Msa0711130:CDS
GAGAAGAAGAACTTCGTTTG+CGG 0.477402 5_1:+2980432 None:intergenic
GCCGTTTGGTTGCGATTACT+GGG 0.478784 5_1:-2980123 Msa0711130:CDS
GGTGAATTATGAGGAGGATG+AGG 0.479462 5_1:-2979873 Msa0711130:CDS
TTCGTGATGACGAAGAATCC+TGG 0.480391 5_1:+2980363 None:intergenic
GGTAGGAGAGGAGAGGGTCC+AGG 0.480489 5_1:+2980521 None:intergenic
GAGTTTATGTGGACTGTTGG+CGG 0.482690 5_1:-2978491 Msa0711130:CDS
GAAGCGGGTTTAGGAGGAGG+AGG 0.484156 5_1:-2979899 Msa0711130:CDS
CCGAATTGCCGGTACGGCGG+CGG 0.484334 5_1:+2980666 None:intergenic
CGTCAATGGCTGGTTGTTGA+CGG 0.484415 5_1:-2980625 Msa0711130:CDS
TTGGAAGCGGGTTTAGGAGG+AGG 0.490530 5_1:-2979902 Msa0711130:CDS
GGGTTTAGGAGGAGGAGGAG+AGG 0.493637 5_1:-2979894 Msa0711130:CDS
TGGTCAACATGACTCCCATT+TGG 0.493890 5_1:+2978612 None:intergenic
GAAGCACAGGTGATCGAGGC+CGG 0.494788 5_1:-2980595 Msa0711130:CDS
AAGGTACAAGCGTTCGTCAA+TGG 0.506236 5_1:-2980639 Msa0711130:CDS
CACGCCATCATGCGTCGTAC+CGG 0.508071 5_1:-2980568 Msa0711130:CDS
TTATCATGAACATCATGATC+AGG 0.508556 5_1:+2979968 None:intergenic
AGGATTCGAAGGTCACGTGC+AGG 0.509325 5_1:+2980541 None:intergenic
TGAGTGAAGAAGAGTAGCGT+AGG 0.509949 5_1:-2980767 Msa0711130:five_prime_UTR
TGGGACCATATTCTTGTATG+TGG 0.512820 5_1:-2978459 Msa0711130:CDS
CAGAACCATCTTCTCCAAAT+GGG 0.517721 5_1:-2978626 Msa0711130:CDS
TTTGGTTGCGATTACTGGGA+GGG 0.518348 5_1:-2980119 Msa0711130:CDS
TCGTCAGATTAAGAGCCGTT+TGG 0.523284 5_1:-2980137 Msa0711130:CDS
TCGTTCAAGAGCTTCAAGCC+AGG 0.526920 5_1:-2980381 Msa0711130:CDS
TACAAGCGTTCGTCAATGGC+TGG 0.527925 5_1:-2980635 Msa0711130:CDS
TGCTGAGATATCGTTGGAAG+CGG 0.529462 5_1:-2979915 Msa0711130:CDS
AAGGTCACGTGCAGGGAGAC+CGG 0.534275 5_1:+2980549 None:intergenic
TGACGGCACCGGCGAAGCAC+AGG 0.535467 5_1:-2980608 Msa0711130:CDS
TGGCTGGTTGTTGACGGCAC+CGG 0.538585 5_1:-2980619 Msa0711130:CDS
ACGCCAACGGTAGCGATGAC+CGG 0.540694 5_1:-2980702 Msa0711130:CDS
TCCCAGTAATCGCAACCAAA+CGG 0.543100 5_1:+2980122 None:intergenic
TCTCTTCCGAGAACGGTGGA+AGG 0.543564 5_1:+2980499 None:intergenic
TTCAACAGTGTCTTCATCAG+AGG 0.545221 5_1:+2979994 None:intergenic
GAAGTCAATTTATCAAGCGC+AGG 0.545824 5_1:+2980190 None:intergenic
GCTGAGATATCGTTGGAAGC+GGG 0.545841 5_1:-2979914 Msa0711130:CDS
CACCACTAAGCTTGATGTTG+AGG 0.552846 5_1:-2979762 Msa0711130:CDS
TCCAGCGCAACGAATTCAAA+CGG 0.553760 5_1:+2980280 None:intergenic
GGAGAGGGTCCAGGATTCGA+AGG 0.557929 5_1:+2980530 None:intergenic
TTTCTTCCGAATTGCCGGTA+CGG 0.558235 5_1:+2980660 None:intergenic
AAGATGCGAGGAAACGTCAC+CGG 0.560599 5_1:-2980727 Msa0711130:CDS
ATGGGATGAGAGAGTTTATG+TGG 0.562365 5_1:-2978502 Msa0711130:CDS
CCACCGGTCATCGCTACCGT+TGG 0.563218 5_1:+2980699 None:intergenic
CTCTTGAACGAAAGGAGTAA+CGG 0.563746 5_1:+2980393 None:intergenic
TCGTTGGAAGCGGGTTTAGG+AGG 0.564325 5_1:-2979905 Msa0711130:CDS
GCTTACTTTGTTCAGATCGA+CGG 0.566014 5_1:-2979719 Msa0711130:CDS
AAAACAAACATAATGTACAC+GGG 0.571581 5_1:+2978146 None:intergenic
GCTTGAAGCTCTTGAACGAA+AGG 0.572210 5_1:+2980385 None:intergenic
CTCTTCCGAGAACGGTGGAA+GGG 0.575974 5_1:+2980500 None:intergenic
GTACCGGCAATTCGGAAGAA+AGG 0.580132 5_1:-2980658 Msa0711130:CDS
TGGAAGGGTAGGAGAGGAGA+GGG 0.581421 5_1:+2980515 None:intergenic
ACCAGGTGCTGAGATATCGT+TGG 0.584563 5_1:-2979921 Msa0711130:CDS
AGGAGAGGTGAATTATGAGG+AGG 0.586365 5_1:-2979879 Msa0711130:CDS
CGGCGGCAGCTGTTCCCCAC+CGG 0.591719 5_1:+2980683 None:intergenic
AGAGAGTTTATGTGGACTGT+TGG 0.592926 5_1:-2978494 Msa0711130:CDS
CAACGGTAGCGATGACCGGT+GGG 0.594792 5_1:-2980698 Msa0711130:CDS
GGAGAGTGAAGCAAAGACGC+TGG 0.597867 5_1:-2980227 Msa0711130:CDS
GAGACCGGTACGACGCATGA+TGG 0.600821 5_1:+2980564 None:intergenic
TTTATGTGGACTGTTGGCGG+CGG 0.601940 5_1:-2978488 Msa0711130:CDS
AGCAAAGACGCTGGAACAAG+AGG 0.602892 5_1:-2980218 Msa0711130:CDS
CTTCCGAATTGCCGGTACGG+CGG 0.605828 5_1:+2980663 None:intergenic
CACTCTCAATAAGCTCTCAA+CGG 0.611809 5_1:-2979696 Msa0711130:intron
GGATTCGAAGGTCACGTGCA+GGG 0.614484 5_1:+2980542 None:intergenic
ATTATCAGGACGAGGAGTAG+TGG 0.614768 5_1:+2980336 None:intergenic
CGGCGAAGCACAGGTGATCG+AGG 0.615701 5_1:-2980599 Msa0711130:CDS
TCCAACGATATCTCAGCACC+TGG 0.620597 5_1:+2979920 None:intergenic
AACGTCACCGGTAACGCCAA+CGG 0.620696 5_1:-2980715 Msa0711130:CDS
TCCGAGAACGGTGGAAGGGT+AGG 0.621308 5_1:+2980504 None:intergenic
TGTTTGATGATAAATTGTAT+GGG 0.621429 5_1:-2978520 Msa0711130:CDS
GAACGGTGGAAGGGTAGGAG+AGG 0.622247 5_1:+2980509 None:intergenic
CCAACGGTAGCGATGACCGG+TGG 0.624688 5_1:-2980699 Msa0711130:CDS
GTTGACCACGGCAACTCTGG+TGG 0.627812 5_1:-2978597 Msa0711130:CDS
CTCAACATCAAGCTTAGTGG+TGG 0.628847 5_1:+2979763 None:intergenic
TCTTCCTGATTATCAGGACG+AGG 0.628896 5_1:+2980328 None:intergenic
TAGTGCTTGCACGTGTTCCG+CGG 0.631370 5_1:+2979798 None:intergenic
GTTTGGTTGCGATTACTGGG+AGG 0.636774 5_1:-2980120 Msa0711130:CDS
GTGGAAGGGTAGGAGAGGAG+AGG 0.646101 5_1:+2980514 None:intergenic
TGAACGAAAGGAGTAACGGA+AGG 0.650529 5_1:+2980397 None:intergenic
ACTTACCACCAGAGTTGCCG+TGG 0.664332 5_1:+2978592 None:intergenic
GATGTTAATCAAACTGGACC+AGG 0.673948 5_1:-2979938 Msa0711130:CDS
AATACCAGCAACAACAACGA+AGG 0.674729 5_1:+2978568 None:intergenic
AACGGTAGCGATGACCGGTG+GGG 0.675836 5_1:-2980697 Msa0711130:CDS
GATAATCAGGAAGATTCTCA+CGG 0.676638 5_1:-2980319 Msa0711130:CDS
GAAGCAAATAGATGTCGTAG+AGG 0.679419 5_1:+2978306 None:intergenic
TCTTTCTCTTCCGAGAACGG+TGG 0.682175 5_1:+2980495 None:intergenic
ACATTATTTATGTAGCACAA+CGG 0.683769 5_1:+2978387 None:intergenic
AATGGGAGTCATGTTGACCA+CGG 0.693986 5_1:-2978609 Msa0711130:CDS
AAACAAACATAATGTACACG+GGG 0.696153 5_1:+2978147 None:intergenic
TCGAATTTAAGGGATAGCCG+CGG 0.713074 5_1:-2979815 Msa0711130:CDS
TGATGATGATGAAGATATGG+CGG 0.716447 5_1:-2980062 Msa0711130:CDS
GGAGTATGTTGATGACACAG+AGG 0.741575 5_1:-2978678 Msa0711130:CDS
AATCAGGAAGATTCTCACGG+CGG 0.760544 5_1:-2980316 Msa0711130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATATGAGTATATATTA+TGG - chr5_1:2980083-2980102 Msa0711130:CDS 10.0%
!! AAATCAAGGAAATGTTAAAT+AGG + chr5_1:2979278-2979297 None:intergenic 20.0%
!!! TTTAACATTTCCTTGATTTT+AGG - chr5_1:2979279-2979298 Msa0711130:intron 20.0%
!! AGATAATTATAATCAATCCA+AGG + chr5_1:2979780-2979799 None:intergenic 20.0%
!! TATGAAAATTGTATCTTCAT+AGG + chr5_1:2979809-2979828 None:intergenic 20.0%
!!! TGATTTTTCATAAACTAACT+TGG - chr5_1:2979866-2979885 Msa0711130:CDS 20.0%
!! GATAAATTATAATCAATCCA+AGG + chr5_1:2980051-2980070 None:intergenic 20.0%
!! TAATAGCAATTATATAAACC+AGG + chr5_1:2980116-2980135 None:intergenic 20.0%
!!! TGTTTGATGATAAATTGTAT+GGG - chr5_1:2980421-2980440 Msa0711130:CDS 20.0%
!!! AATTACAATTTTACAGAACA+CGG + chr5_1:2980606-2980625 None:intergenic 20.0%
!! AAAAACAAACATAATGTACA+CGG + chr5_1:2980799-2980818 None:intergenic 20.0%
! GTTATAGAAACCTAAAATCA+AGG + chr5_1:2979292-2979311 None:intergenic 25.0%
!!! CATTAGTGTTTTTAACTTGT+TGG + chr5_1:2979338-2979357 None:intergenic 25.0%
! AAAAATAAGCCAATCCAAAA+GGG + chr5_1:2979612-2979631 None:intergenic 25.0%
! AAAAAATAAGCCAATCCAAA+AGG + chr5_1:2979613-2979632 None:intergenic 25.0%
! ATATATTATGGTACAATACC+TGG - chr5_1:2980095-2980114 Msa0711130:CDS 25.0%
! TATGTGATATGATGTTACTA+TGG - chr5_1:2980142-2980161 Msa0711130:CDS 25.0%
!! CTGTTTGATGATAAATTGTA+TGG - chr5_1:2980420-2980439 Msa0711130:CDS 25.0%
! ACATTATTTATGTAGCACAA+CGG + chr5_1:2980557-2980576 None:intergenic 25.0%
! GTACAAGAAAAATTAGTTGA+AGG + chr5_1:2980676-2980695 None:intergenic 25.0%
!! TTTTCTTGTACATTGTTGTA+TGG - chr5_1:2980685-2980704 Msa0711130:CDS 25.0%
! AAAACAAACATAATGTACAC+GGG + chr5_1:2980798-2980817 None:intergenic 25.0%
TCGAATTCCACACAAAAAAT+TGG - chr5_1:2978143-2978162 Msa0711130:three_prime_UTR 30.0%
CGAATTCCACACAAAAAATT+GGG - chr5_1:2978144-2978163 Msa0711130:three_prime_UTR 30.0%
GAAGATTAGTACTCTCAATT+TGG - chr5_1:2978774-2978793 Msa0711130:intron 30.0%
TCAAAAAGTGAGAGATGAAT+TGG - chr5_1:2978846-2978865 Msa0711130:intron 30.0%
TTATCATGAACATCATGATC+AGG + chr5_1:2978976-2978995 None:intergenic 30.0%
GATGTTGATGTTAATCAAAC+TGG - chr5_1:2978997-2979016 Msa0711130:intron 30.0%
! AATTCTAGAGAGCAAATGTT+TGG - chr5_1:2979099-2979118 Msa0711130:intron 30.0%
CAAGGAAATGTTAAATAGGA+AGG + chr5_1:2979274-2979293 None:intergenic 30.0%
! TTTTTGCTTTCTGATCTAGT+TGG - chr5_1:2979377-2979396 Msa0711130:intron 30.0%
TATGTCACTAGATAAGTTCA+AGG - chr5_1:2979585-2979604 Msa0711130:intron 30.0%
ATGTCACTAGATAAGTTCAA+GGG - chr5_1:2979586-2979605 Msa0711130:intron 30.0%
AAATCAGCTTATGAAGTAGT+AGG - chr5_1:2979705-2979724 Msa0711130:CDS 30.0%
! TCTTTGGATTGACTTATTTG+GGG + chr5_1:2979975-2979994 None:intergenic 30.0%
! CTCTTTGGATTGACTTATTT+GGG + chr5_1:2979976-2979995 None:intergenic 30.0%
! TCTCTTTGGATTGACTTATT+TGG + chr5_1:2979977-2979996 None:intergenic 30.0%
!! GCTGATACTTTGTTATTTGT+TGG - chr5_1:2980221-2980240 Msa0711130:CDS 30.0%
AAACAAACATAATGTACACG+GGG + chr5_1:2980797-2980816 None:intergenic 30.0%
!!! TTCGTTCCCAATTTTTTGTG+TGG + chr5_1:2978153-2978172 None:intergenic 35.0%
CAGAAAAAATCAAAGATGCG+AGG - chr5_1:2978202-2978221 Msa0711130:three_prime_UTR 35.0%
TGAGAATCTTCCTGATTATC+AGG + chr5_1:2978622-2978641 None:intergenic 35.0%
GATAATCAGGAAGATTCTCA+CGG - chr5_1:2978622-2978641 Msa0711130:CDS 35.0%
!! GCTTGATGATGATGAAGATA+TGG - chr5_1:2978876-2978895 Msa0711130:intron 35.0%
! TGATGATGATGAAGATATGG+CGG - chr5_1:2978879-2978898 Msa0711130:intron 35.0%
!! TGTTTGGTGCTTCGAATTTA+AGG - chr5_1:2979115-2979134 Msa0711130:intron 35.0%
!! GTTTGGTGCTTCGAATTTAA+GGG - chr5_1:2979116-2979135 Msa0711130:intron 35.0%
!!! GTGTTTTTAACTTGTTGGAC+TGG + chr5_1:2979333-2979352 None:intergenic 35.0%
CTTATTTCCACAAACTCACT+AGG + chr5_1:2979497-2979516 None:intergenic 35.0%
! CACTTTATGGTTTGTACTTG+AGG - chr5_1:2979754-2979773 Msa0711130:CDS 35.0%
CAAAAATAAGCCGATCCAAA+CGG - chr5_1:2979947-2979966 Msa0711130:CDS 35.0%
AAAAATAAGCCGATCCAAAC+GGG - chr5_1:2979948-2979967 Msa0711130:CDS 35.0%
! CAAAGTATCAGCAACAAAGA+AGG + chr5_1:2980213-2980232 None:intergenic 35.0%
!! GTTGTTGTTGCTGGTATTTT+TGG - chr5_1:2980378-2980397 Msa0711130:CDS 35.0%
AGCAACCACATACAAGAATA+TGG + chr5_1:2980490-2980509 None:intergenic 35.0%
GAGAAGAAGAACTTCGTTTG+CGG + chr5_1:2978512-2978531 None:intergenic 40.0%
CTCTTGAACGAAAGGAGTAA+CGG + chr5_1:2978551-2978570 None:intergenic 40.0%
! TGAAGCAGCTTGTAGTGTTT+TGG - chr5_1:2978693-2978712 Msa0711130:intron 40.0%
GAAGTCAATTTATCAAGCGC+AGG + chr5_1:2978754-2978773 None:intergenic 40.0%
GAAGTCACTTCAACAGCAAT+TGG - chr5_1:2978918-2978937 Msa0711130:intron 40.0%
TTCAACAGTGTCTTCATCAG+AGG + chr5_1:2978950-2978969 None:intergenic 40.0%
GATGTTAATCAAACTGGACC+AGG - chr5_1:2979003-2979022 Msa0711130:intron 40.0%
GCTTACTTTGTTCAGATCGA+CGG - chr5_1:2979222-2979241 Msa0711130:intron 40.0%
CACTCTCAATAAGCTCTCAA+CGG - chr5_1:2979245-2979264 Msa0711130:intron 40.0%
ATGCTATCCTAGTGAGTTTG+TGG - chr5_1:2979487-2979506 Msa0711130:intron 40.0%
ATCGGTACTAAGAGTCTCTT+TGG + chr5_1:2979991-2980010 None:intergenic 40.0%
ACAGAACCATCTTCTCCAAA+TGG - chr5_1:2980314-2980333 Msa0711130:CDS 40.0%
CAGAACCATCTTCTCCAAAT+GGG - chr5_1:2980315-2980334 Msa0711130:CDS 40.0%
AATACCAGCAACAACAACGA+AGG + chr5_1:2980376-2980395 None:intergenic 40.0%
ATGGGATGAGAGAGTTTATG+TGG - chr5_1:2980439-2980458 Msa0711130:CDS 40.0%
! AGAGAGTTTATGTGGACTGT+TGG - chr5_1:2980447-2980466 Msa0711130:CDS 40.0%
TGGGACCATATTCTTGTATG+TGG - chr5_1:2980482-2980501 Msa0711130:CDS 40.0%
GAAGCAAATAGATGTCGTAG+AGG + chr5_1:2980638-2980657 None:intergenic 40.0%
TGAGTGAAGAAGAGTAGCGT+AGG - chr5_1:2978174-2978193 Msa0711130:three_prime_UTR 45.0%
TTTCTTCCGAATTGCCGGTA+CGG + chr5_1:2978284-2978303 None:intergenic 45.0%
GTACCTTTCTTCCGAATTGC+CGG + chr5_1:2978289-2978308 None:intergenic 45.0%
AAGGTACAAGCGTTCGTCAA+TGG - chr5_1:2978302-2978321 Msa0711130:three_prime_UTR 45.0%
TGCTCTTTCTCTTCCGAGAA+CGG + chr5_1:2978452-2978471 None:intergenic 45.0%
! TGAACGAAAGGAGTAACGGA+AGG + chr5_1:2978547-2978566 None:intergenic 45.0%
GCTTGAAGCTCTTGAACGAA+AGG + chr5_1:2978559-2978578 None:intergenic 45.0%
! TTCGTGATGACGAAGAATCC+TGG + chr5_1:2978581-2978600 None:intergenic 45.0%
ATTATCAGGACGAGGAGTAG+TGG + chr5_1:2978608-2978627 None:intergenic 45.0%
TACTCCTCGTCCTGATAATC+AGG - chr5_1:2978609-2978628 Msa0711130:CDS 45.0%
TCTTCCTGATTATCAGGACG+AGG + chr5_1:2978616-2978635 None:intergenic 45.0%
AATCAGGAAGATTCTCACGG+CGG - chr5_1:2978625-2978644 Msa0711130:CDS 45.0%
TCCAGCGCAACGAATTCAAA+CGG + chr5_1:2978664-2978683 None:intergenic 45.0%
TCGTCAGATTAAGAGCCGTT+TGG - chr5_1:2978804-2978823 Msa0711130:intron 45.0%
TCCCAGTAATCGCAACCAAA+CGG + chr5_1:2978822-2978841 None:intergenic 45.0%
TTTGGTTGCGATTACTGGGA+GGG - chr5_1:2978822-2978841 Msa0711130:intron 45.0%
TGCTGAGATATCGTTGGAAG+CGG - chr5_1:2979026-2979045 Msa0711130:intron 45.0%
! ATATCGTTGGAAGCGGGTTT+AGG - chr5_1:2979033-2979052 Msa0711130:intron 45.0%
AGGAGGAGAGGTGAATTATG+AGG - chr5_1:2979059-2979078 Msa0711130:intron 45.0%
AGGAGAGGTGAATTATGAGG+AGG - chr5_1:2979062-2979081 Msa0711130:intron 45.0%
GGTGAATTATGAGGAGGATG+AGG - chr5_1:2979068-2979087 Msa0711130:intron 45.0%
TCGAATTTAAGGGATAGCCG+CGG - chr5_1:2979126-2979145 Msa0711130:intron 45.0%
CTCAACATCAAGCTTAGTGG+TGG + chr5_1:2979181-2979200 None:intergenic 45.0%
CACCACTAAGCTTGATGTTG+AGG - chr5_1:2979179-2979198 Msa0711130:intron 45.0%
CTCCTCAACATCAAGCTTAG+TGG + chr5_1:2979184-2979203 None:intergenic 45.0%
! GATAAGTTCAAGGGCCCTTT+TGG - chr5_1:2979595-2979614 Msa0711130:intron 45.0%
! ATGGTTTGTACTTGAGGCCT+TGG - chr5_1:2979760-2979779 Msa0711130:CDS 45.0%
!! TCTTAGTACCGATTACCACG+AGG - chr5_1:2979998-2980017 Msa0711130:CDS 45.0%
AGTCTTCTCCTCGTGGTAAT+CGG + chr5_1:2980009-2980028 None:intergenic 45.0%
! GACTTGATGGTCTGTACTTG+AGG - chr5_1:2980025-2980044 Msa0711130:CDS 45.0%
! TGGTTGTCTTGTTGAAGCTG+AGG - chr5_1:2980241-2980260 Msa0711130:CDS 45.0%
! GGTTGTCTTGTTGAAGCTGA+GGG - chr5_1:2980242-2980261 Msa0711130:CDS 45.0%
!! GGAGTATGTTGATGACACAG+AGG - chr5_1:2980263-2980282 Msa0711130:CDS 45.0%
TGACTCCCATTTGGAGAAGA+TGG + chr5_1:2980323-2980342 None:intergenic 45.0%
TGGTCAACATGACTCCCATT+TGG + chr5_1:2980332-2980351 None:intergenic 45.0%
AATGGGAGTCATGTTGACCA+CGG - chr5_1:2980332-2980351 Msa0711130:CDS 45.0%
! GAGTTTATGTGGACTGTTGG+CGG - chr5_1:2980450-2980469 Msa0711130:CDS 45.0%
! TGTATGTGGTTGCTATTGCC+TGG - chr5_1:2980496-2980515 Msa0711130:CDS 45.0%
AAGATGCGAGGAAACGTCAC+CGG - chr5_1:2978214-2978233 Msa0711130:three_prime_UTR 50.0%
! GTACCGGCAATTCGGAAGAA+AGG - chr5_1:2978283-2978302 Msa0711130:three_prime_UTR 50.0%
TACAAGCGTTCGTCAATGGC+TGG - chr5_1:2978306-2978325 Msa0711130:three_prime_UTR 50.0%
! CGTCAATGGCTGGTTGTTGA+CGG - chr5_1:2978316-2978335 Msa0711130:three_prime_UTR 50.0%
!! ACGCATGATGGCGTGTTTTC+CGG + chr5_1:2978368-2978387 None:intergenic 50.0%
TCTTTCTCTTCCGAGAACGG+TGG + chr5_1:2978449-2978468 None:intergenic 50.0%
TCGTTCAAGAGCTTCAAGCC+AGG - chr5_1:2978560-2978579 Msa0711130:CDS 50.0%
TCCGTTTGAATTCGTTGCGC+TGG - chr5_1:2978660-2978679 Msa0711130:CDS 50.0%
AGCAAAGACGCTGGAACAAG+AGG - chr5_1:2978723-2978742 Msa0711130:intron 50.0%
AGCCGTTTGGTTGCGATTAC+TGG - chr5_1:2978817-2978836 Msa0711130:intron 50.0%
GCCGTTTGGTTGCGATTACT+GGG - chr5_1:2978818-2978837 Msa0711130:intron 50.0%
GTTTGGTTGCGATTACTGGG+AGG - chr5_1:2978821-2978840 Msa0711130:intron 50.0%
!! ACCAGGTGCTGAGATATCGT+TGG - chr5_1:2979020-2979039 Msa0711130:intron 50.0%
TCCAACGATATCTCAGCACC+TGG + chr5_1:2979024-2979043 None:intergenic 50.0%
GCTGAGATATCGTTGGAAGC+GGG - chr5_1:2979027-2979046 Msa0711130:intron 50.0%
TGAGGAGCTTGAGATGCTCT+TGG - chr5_1:2979197-2979216 Msa0711130:intron 50.0%
! GTTCAAGGGCCCTTTTGGAT+TGG - chr5_1:2979600-2979619 Msa0711130:intron 50.0%
! ACCATAAAGTGCTCTCCGCT+CGG + chr5_1:2979745-2979764 None:intergenic 50.0%
! ATTTGGGGTCCCGTTTGGAT+CGG + chr5_1:2979960-2979979 None:intergenic 50.0%
! GACTTATTTGGGGTCCCGTT+TGG + chr5_1:2979965-2979984 None:intergenic 50.0%
ACCACGAGGAGAAGACTTGA+TGG - chr5_1:2980012-2980031 Msa0711130:CDS 50.0%
ACCATCAAGTCTTCTCCTCG+TGG + chr5_1:2980016-2980035 None:intergenic 50.0%
ATGGTCTGTACTTGAGGCCT+TGG - chr5_1:2980031-2980050 Msa0711130:CDS 50.0%
! AGTGCCTTCGTTGTTGTTGC+TGG - chr5_1:2980369-2980388 Msa0711130:CDS 50.0%
! TTTATGTGGACTGTTGGCGG+CGG - chr5_1:2980453-2980472 Msa0711130:CDS 50.0%
! CAAACGCTTGCTGGAGTGAA+TGG - chr5_1:2980527-2980546 Msa0711130:CDS 50.0%
AACGTCACCGGTAACGCCAA+CGG - chr5_1:2978226-2978245 Msa0711130:three_prime_UTR 55.0%
ATCGCTACCGTTGGCGTTAC+CGG + chr5_1:2978236-2978255 None:intergenic 55.0%
GGATTCGAAGGTCACGTGCA+GGG + chr5_1:2978402-2978421 None:intergenic 55.0%
AGGATTCGAAGGTCACGTGC+AGG + chr5_1:2978403-2978422 None:intergenic 55.0%
TGCACGTGACCTTCGAATCC+TGG - chr5_1:2978402-2978421 Msa0711130:exon 55.0%
TGGAAGGGTAGGAGAGGAGA+GGG + chr5_1:2978429-2978448 None:intergenic 55.0%
TCCTACCCTTCCACCGTTCT+CGG - chr5_1:2978436-2978455 Msa0711130:CDS 55.0%
CTCTTCCGAGAACGGTGGAA+GGG + chr5_1:2978444-2978463 None:intergenic 55.0%
TCTCTTCCGAGAACGGTGGA+AGG + chr5_1:2978445-2978464 None:intergenic 55.0%
GGAGAGTGAAGCAAAGACGC+TGG - chr5_1:2978714-2978733 Msa0711130:intron 55.0%
! TCGTTGGAAGCGGGTTTAGG+AGG - chr5_1:2979036-2979055 Msa0711130:intron 55.0%
! TTGGAAGCGGGTTTAGGAGG+AGG - chr5_1:2979039-2979058 Msa0711130:intron 55.0%
TAGTGCTTGCACGTGTTCCG+CGG + chr5_1:2979146-2979165 None:intergenic 55.0%
!! ACCGAGCGGAGAGCACTTTA+TGG - chr5_1:2979741-2979760 Msa0711130:CDS 55.0%
CATGTTGACCACGGCAACTC+TGG - chr5_1:2980341-2980360 Msa0711130:CDS 55.0%
ACTTACCACCAGAGTTGCCG+TGG + chr5_1:2980352-2980371 None:intergenic 55.0%
CAAGCGTTTGTGCTTGCACC+AGG + chr5_1:2980517-2980536 None:intergenic 55.0%
GTGCAAGCACAAACGCTTGC+TGG - chr5_1:2980518-2980537 Msa0711130:CDS 55.0%
ACGCCAACGGTAGCGATGAC+CGG - chr5_1:2978239-2978258 Msa0711130:three_prime_UTR 60.0%
CAACGGTAGCGATGACCGGT+GGG - chr5_1:2978243-2978262 Msa0711130:three_prime_UTR 60.0%
AACGGTAGCGATGACCGGTG+GGG - chr5_1:2978244-2978263 Msa0711130:three_prime_UTR 60.0%
CTTCCGAATTGCCGGTACGG+CGG + chr5_1:2978281-2978300 None:intergenic 60.0%
! TGGCTGGTTGTTGACGGCAC+CGG - chr5_1:2978322-2978341 Msa0711130:three_prime_UTR 60.0%
CTCGATCACCTGTGCTTCGC+CGG + chr5_1:2978344-2978363 None:intergenic 60.0%
GAAGCACAGGTGATCGAGGC+CGG - chr5_1:2978346-2978365 Msa0711130:three_prime_UTR 60.0%
CACGCCATCATGCGTCGTAC+CGG - chr5_1:2978373-2978392 Msa0711130:three_prime_UTR 60.0%
GAGACCGGTACGACGCATGA+TGG + chr5_1:2978380-2978399 None:intergenic 60.0%
AAGGTCACGTGCAGGGAGAC+CGG + chr5_1:2978395-2978414 None:intergenic 60.0%
GGAGAGGGTCCAGGATTCGA+AGG + chr5_1:2978414-2978433 None:intergenic 60.0%
GTGGAAGGGTAGGAGAGGAG+AGG + chr5_1:2978430-2978449 None:intergenic 60.0%
GAACGGTGGAAGGGTAGGAG+AGG + chr5_1:2978435-2978454 None:intergenic 60.0%
TCCGAGAACGGTGGAAGGGT+AGG + chr5_1:2978440-2978459 None:intergenic 60.0%
! GAAGCGGGTTTAGGAGGAGG+AGG - chr5_1:2979042-2979061 Msa0711130:intron 60.0%
GGGTTTAGGAGGAGGAGGAG+AGG - chr5_1:2979047-2979066 Msa0711130:intron 60.0%
!! GACGAGTACCGACTACCGAG+CGG - chr5_1:2979727-2979746 Msa0711130:CDS 60.0%
AGTGCTCTCCGCTCGGTAGT+CGG + chr5_1:2979738-2979757 None:intergenic 60.0%
GTTGACCACGGCAACTCTGG+TGG - chr5_1:2980344-2980363 Msa0711130:CDS 60.0%
CCACCGGTCATCGCTACCGT+TGG + chr5_1:2978245-2978264 None:intergenic 65.0%
CCAACGGTAGCGATGACCGG+TGG - chr5_1:2978242-2978261 Msa0711130:three_prime_UTR 65.0%
CGGCGAAGCACAGGTGATCG+AGG - chr5_1:2978342-2978361 Msa0711130:three_prime_UTR 65.0%
GGTAGGAGAGGAGAGGGTCC+AGG + chr5_1:2978423-2978442 None:intergenic 65.0%
AACAGCTGCCGCCGCCGTAC+CGG - chr5_1:2978267-2978286 Msa0711130:three_prime_UTR 70.0%
CCGAATTGCCGGTACGGCGG+CGG + chr5_1:2978278-2978297 None:intergenic 70.0%
! CCGCCGCCGTACCGGCAATT+CGG - chr5_1:2978275-2978294 Msa0711130:three_prime_UTR 70.0%
!! TGACGGCACCGGCGAAGCAC+AGG - chr5_1:2978333-2978352 Msa0711130:three_prime_UTR 70.0%
ACTGTTGGCGGCGGCACTGC+TGG - chr5_1:2980462-2980481 Msa0711130:CDS 70.0%
CTGTTGGCGGCGGCACTGCT+GGG - chr5_1:2980463-2980482 Msa0711130:CDS 70.0%
CGGCGGCAGCTGTTCCCCAC+CGG + chr5_1:2978261-2978280 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 2978122 2980841 2978122 ID=Msa0711130;Name=Msa0711130
chr5_1 mRNA 2978122 2980841 2978122 ID=Msa0711130-mRNA-1;Parent=Msa0711130;Name=Msa0711130-mRNA-1;_AED=0.01;_eAED=0.01;_QI=95|1|1|1|1|1|2|296|445
chr5_1 exon 2978122 2978705 2978122 ID=Msa0711130-mRNA-1:exon:992;Parent=Msa0711130-mRNA-1
chr5_1 exon 2979697 2980841 2979697 ID=Msa0711130-mRNA-1:exon:991;Parent=Msa0711130-mRNA-1
chr5_1 five_prime_UTR 2980747 2980841 2980747 ID=Msa0711130-mRNA-1:five_prime_utr;Parent=Msa0711130-mRNA-1
chr5_1 CDS 2979697 2980746 2979697 ID=Msa0711130-mRNA-1:cds;Parent=Msa0711130-mRNA-1
chr5_1 CDS 2978418 2978705 2978418 ID=Msa0711130-mRNA-1:cds;Parent=Msa0711130-mRNA-1
chr5_1 three_prime_UTR 2978122 2978417 2978122 ID=Msa0711130-mRNA-1:three_prime_utr;Parent=Msa0711130-mRNA-1
Gene Sequence

>Msa0711130

ATGCGAGGAAACGTCACCGGTAACGCCAACGGTAGCGATGACCGGTGGGGAACAGCTGCCGCCGCCGTACCGGCAATTCGGAAGAAAGGTACAAGCGTTCGTCAATGGCTGGTTGTTGACGGCACCGGCGAAGCACAGGTGATCGAGGCCGGAAAACACGCCATCATGCGTCGTACCGGTCTCCCTGCACGTGACCTTCGAATCCTGGACCCTCTCCTCTCCTACCCTTCCACCGTTCTCGGAAGAGAAAGAGCAATCGTCATCAATTTAGAACACATCAAAGCAATTATCACCGCAAACGAAGTTCTTCTTCTCAATTCACGCGATCCTTCCGTTACTCCTTTCGTTCAAGAGCTTCAAGCCAGGATTCTTCGTCATCACGAAGCAACCACTACTCCTCGTCCTGATAATCAGGAAGATTCTCACGGCGGAATTAAGATTCTTCCGTTTGAATTCGTTGCGCTGGAAGCGTGTCTTGAAGCAGCTTGTAGTGTTTTGGAGAGTGAAGCAAAGACGCTGGAACAAGAGGCGCATCCTGCGCTTGATAAATTGACTTCGAAGATTAGTACTCTCAATTTGGAACGTGTTCGTCAGATTAAGAGCCGTTTGGTTGCGATTACTGGGAGGGTTCAAAAAGTGAGAGATGAATTGGAACACTTGCTTGATGATGATGAAGATATGGCGGAGATGTATCTTACTGAGAAGTCACTTCAACAGCAATTGGAACAAACCTCTGATGAAGACACTGTTGAAACTCCTGATCATGATGTTCATGATAATGATGTTGATGTTAATCAAACTGGACCAGGTGCTGAGATATCGTTGGAAGCGGGTTTAGGAGGAGGAGGAGAGGTGAATTATGAGGAGGATGAGGATAATCTGAATTCTAGAGAGCAAATGTTTGGTGCTTCGAATTTAAGGGATAGCCGCGGAACACGTGCAAGCACTACTTATAGTGTCACCACCACTAAGCTTGATGTTGAGGAGCTTGAGATGCTCTTGGAAGCTTACTTTGTTCAGATCGACGGCACTCTCAATAAGCTCTCAACGCTGAGGGAGTATGTTGATGACACAGAGGATTATATCAACATTATGCTTGATGACAAACAGAACCATCTTCTCCAAATGGGAGTCATGTTGACCACGGCAACTCTGGTGGTAAGTGCCTTCGTTGTTGTTGCTGGTATTTTTGGCATGAATATTAAAATTGAACTGTTTGATGATAAATTGTATGGGATGAGAGAGTTTATGTGGACTGTTGGCGGCGGCACTGCTGGGACCATATTCTTGTATGTGGTTGCTATTGCCTGGTGCAAGCACAAACGCTTGCTGGAGTGA
Protein sequence

>Msa0711130

MRGNVTGNANGSDDRWGTAAAAVPAIRKKGTSVRQWLVVDGTGEAQVIEAGKHAIMRRTGLPARDLRILDPLLSYPSTVLGRERAIVINLEHIKAIITANEVLLLNSRDPSVTPFVQELQARILRHHEATTTPRPDNQEDSHGGIKILPFEFVALEACLEAACSVLESEAKTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDELEHLLDDDEDMAEMYLTEKSLQQQLEQTSDEDTVETPDHDVHDNDVDVNQTGPGAEISLEAGLGGGGEVNYEEDEDNLNSREQMFGASNLRDSRGTRASTTYSVTTTKLDVEELEMLLEAYFVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIKIELFDDKLYGMREFMWTVGGGTAGTIFLYVVAIAWCKHKRLLE*