AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0158130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158130 MtrunA17_Chr1g0177971 100.000 146 0 0 1 146 1 146 8.78e-110 308
Msa0158130 MtrunA17_Chr8g0391521 80.142 141 28 0 3 143 1 141 4.02e-84 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158130 AT3G52560.1 92.466 146 11 0 1 146 1 146 2.13e-102 289
Msa0158130 AT2G36060.1 93.151 146 9 1 1 146 1 145 6.45e-102 288
Msa0158130 AT3G52560.2 91.837 147 11 1 1 146 1 147 1.09e-100 285
Msa0158130 AT2G36060.2 92.517 147 9 2 1 146 1 146 3.39e-100 284
Msa0158130 AT2G36060.3 92.466 146 9 2 1 146 1 144 1.88e-99 282
Msa0158130 AT3G52560.4 82.317 164 11 1 1 146 1 164 3.77e-98 279
Msa0158130 AT1G23260.1 75.177 141 35 0 3 143 1 141 6.83e-80 233
Msa0158130 AT1G70660.2 73.239 142 38 0 3 144 1 142 2.83e-78 228
Msa0158130 AT1G70660.1 73.239 142 38 0 3 144 1 142 5.19e-78 228
Msa0158130 AT3G52560.3 90.000 110 10 1 38 146 1 110 2.25e-73 215
Msa0158130 AT1G70650.2 73.529 136 36 0 8 143 442 577 4.04e-71 224
Msa0158130 AT1G23260.2 72.642 106 29 0 38 143 1 106 1.24e-57 176

Find 90 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACAGCTTTAAGTGATTTA+AGG 0.114327 1_4:+26731294 Msa0158130:three_prime_UTR
CACCAGTGAGATTGCCATTA+TGG 0.188244 1_4:+26731438 Msa0158130:three_prime_UTR
CTGCCATTTCTTTCTTCAGC+TGG 0.199609 1_4:-26730804 None:intergenic
TATGTTTGCACAATTGATTT+TGG 0.213986 1_4:+26731238 Msa0158130:three_prime_UTR
TGTGACTGATTTGTTGAATA+TGG 0.238105 1_4:+26731349 Msa0158130:three_prime_UTR
CAGGTTGAACCAAAGAAATT+TGG 0.239713 1_4:+26730733 Msa0158130:intron
GGGCCTGTTTATTGCGGATT+TGG 0.258334 1_4:+26731260 Msa0158130:three_prime_UTR
GGCAAAATCCCTTGTGCTAT+TGG 0.286709 1_4:+26731459 Msa0158130:three_prime_UTR
AATTTGGGCTTCTTGCAAAT+TGG 0.294147 1_4:+26730749 Msa0158130:CDS
GCAAGAAGCCCAAATTTCTT+TGG 0.297912 1_4:-26730742 None:intergenic
TGCTAAAAGTAAGTTCCTTC+TGG 0.317643 1_4:-26730856 None:intergenic
TGCTCCTATTTCCATCTCTA+TGG 0.319447 1_4:+26730981 Msa0158130:three_prime_UTR
TCCAAGGAACTTCAGATTGC+TGG 0.330124 1_4:+26729660 Msa0158130:CDS
AGAGAAATACTTACATTATG+AGG 0.334105 1_4:-26729790 None:intergenic
TCTTGGACTGGCACCATCAT+TGG 0.337902 1_4:+26729766 Msa0158130:CDS
CTGATACCACGCCAGTTTCA+TGG 0.343773 1_4:-26730549 None:intergenic
GTAAGTATGCTGGAGCTAAA+TGG 0.353602 1_4:+26731182 Msa0158130:three_prime_UTR
CTGGAGGCTGGACAAGCTTC+CGG 0.358439 1_4:-26730837 None:intergenic
AGGTTGAACCAAAGAAATTT+GGG 0.360917 1_4:+26730734 Msa0158130:intron
TACAGCTTTAAGTGATTTAA+GGG 0.363497 1_4:+26731295 Msa0158130:three_prime_UTR
GAGCTTGAACGAGGTGAAAA+AGG 0.363928 1_4:+26729685 Msa0158130:CDS
TCTGAATTCAAGAACATGTT+GGG 0.375311 1_4:+26730955 Msa0158130:three_prime_UTR
GAAGCCATAGAGATGGAAAT+AGG 0.389417 1_4:-26730985 None:intergenic
TGCCATTTCTTTCTTCAGCT+GGG 0.392353 1_4:-26730803 None:intergenic
CTTCTGCCTTGTAAGTATGC+TGG 0.397725 1_4:+26731172 Msa0158130:three_prime_UTR
ATGACATTTACATGCGCTCT+TGG 0.418963 1_4:+26729749 Msa0158130:CDS
AAATGGCAGCCCCTCATAAC+CGG 0.419954 1_4:+26730818 Msa0158130:CDS
TCCATGACGCTTGGCTCAGC+CGG 0.429765 1_4:+26728671 Msa0158130:exon
AATCCAAATCCGCAATAAAC+AGG 0.432969 1_4:-26731263 None:intergenic
ATTTACATGCGCTCTTGGAC+TGG 0.438813 1_4:+26729754 Msa0158130:CDS
TGGACAAGCTTCCGGTTATG+AGG 0.443651 1_4:-26730829 None:intergenic
AGTAAGTTCCTTCTGGAGGC+TGG 0.451073 1_4:-26730849 None:intergenic
TCCAGCAATCTGAAGTTCCT+TGG 0.453074 1_4:-26729661 None:intergenic
CAGAAAAGCCGCCAAGTGTT+CGG 0.455334 1_4:+26730494 Msa0158130:CDS
ATCCAACAACTATACGATCT+TGG 0.459484 1_4:-26730880 None:intergenic
ATATAATGTGTAATGCTTTG+TGG 0.467062 1_4:+26730927 Msa0158130:three_prime_UTR
ATCCAAGATCGTATAGTTGT+TGG 0.470173 1_4:+26730878 Msa0158130:three_prime_UTR
ACTTGTGTTAACCATGAAAC+TGG 0.470899 1_4:+26730538 Msa0158130:CDS
GCAGAAGAAAGTAAAGCAAA+AGG 0.474862 1_4:-26731156 None:intergenic
TACTTTGTCAACTGTGCTGA+AGG 0.478655 1_4:+26731026 Msa0158130:three_prime_UTR
TTAAGGGGTGTCTTGTTCGT+TGG 0.483068 1_4:+26731311 Msa0158130:three_prime_UTR
TGCTGATTTGCTTGACTGCA+GGG 0.487975 1_4:+26731386 Msa0158130:three_prime_UTR
TCACTTAAAGCTGTAGGTGT+AGG 0.490702 1_4:-26731287 None:intergenic
TTCTGAATTCAAGAACATGT+TGG 0.499548 1_4:+26730954 Msa0158130:three_prime_UTR
GTGCTGATTTGCTTGACTGC+AGG 0.500848 1_4:+26731385 Msa0158130:three_prime_UTR
CTAACCGACGACACTGGATC+CGG 0.502436 1_4:-26728690 None:intergenic
TGCCATAATGGCAATCTCAC+TGG 0.505221 1_4:-26731440 None:intergenic
CACTTAAAGCTGTAGGTGTA+GGG 0.509206 1_4:-26731286 None:intergenic
TTGTGGAGAATAAACAAAGA+AGG 0.515036 1_4:-26728576 None:intergenic
CGTGAATGAAACCGAACACT+TGG 0.519764 1_4:-26730505 None:intergenic
GAAAAGAAGAACGCGAATTG+TGG 0.523498 1_4:-26728593 None:intergenic
GGACAAGCTTCCGGTTATGA+GGG 0.527492 1_4:-26730828 None:intergenic
TACATTATGAGGGCCAATGA+TGG 0.529713 1_4:-26729779 None:intergenic
GAATGAAACCGAACACTTGG+CGG 0.532883 1_4:-26730502 None:intergenic
AACATGTTCTTGAATTCAGA+AGG 0.532974 1_4:-26730952 None:intergenic
TCCGGCTGAGCCAAGCGTCA+TGG 0.534232 1_4:-26728672 None:intergenic
CAAAATCCATCCATGACGCT+TGG 0.537360 1_4:+26728662 Msa0158130:exon
GACCCAGCTGAAGAAAGAAA+TGG 0.541436 1_4:+26730801 Msa0158130:CDS
TGTGGAGAATAAACAAAGAA+GGG 0.541776 1_4:-26728575 None:intergenic
TACCATTGTATGTAATCTGA+AGG 0.543246 1_4:-26731112 None:intergenic
GAGAAATACTTACATTATGA+GGG 0.550807 1_4:-26729789 None:intergenic
CAACTCTGAAGCCATAGAGA+TGG 0.564184 1_4:-26730992 None:intergenic
TTCCTTCAGATTACATACAA+TGG 0.566375 1_4:+26731110 Msa0158130:three_prime_UTR
TTAGCTCCAGCATACTTACA+AGG 0.568432 1_4:-26731178 None:intergenic
CTTAAATCACTTAAAGCTGT+AGG 0.572870 1_4:-26731293 None:intergenic
TGGTAACTAACCGACGACAC+TGG 0.573621 1_4:-26728696 None:intergenic
TACTTTCAGACTGTACATGA+AGG 0.578155 1_4:+26730430 Msa0158130:intron
GCTGAGCCAAGCGTCATGGA+TGG 0.584022 1_4:-26728668 None:intergenic
AAAGAAAATGGAGTGCTGAT+CGG 0.587740 1_4:-26728540 None:intergenic
GTTAGCTATGGAATGGATGA+TGG 0.588764 1_4:+26729724 Msa0158130:CDS
CTGAATTCAAGAACATGTTG+GGG 0.589206 1_4:+26730956 Msa0158130:three_prime_UTR
GGTGATGGCACTGTTAGCTA+TGG 0.599683 1_4:+26729712 Msa0158130:CDS
TGCGCTTCAATGCAGTTCCA+AGG 0.600751 1_4:+26729644 Msa0158130:intron
AAGCTTGTCCAGCCTCCAGA+AGG 0.603455 1_4:+26730841 Msa0158130:CDS
AAGGAACTTCAGATTGCTGG+AGG 0.606974 1_4:+26729663 Msa0158130:CDS
GACAAGCTTCCGGTTATGAG+GGG 0.622492 1_4:-26730827 None:intergenic
ACTTTGTCAACTGTGCTGAA+GGG 0.624860 1_4:+26731027 Msa0158130:three_prime_UTR
TCAGCCGGATCCAGTGTCGT+CGG 0.640126 1_4:+26728686 Msa0158130:CDS
TGGCACTGTTAGCTATGGAA+TGG 0.640972 1_4:+26729717 Msa0158130:CDS
ATTTGTTGAATATGGACATG+TGG 0.648408 1_4:+26731357 Msa0158130:three_prime_UTR
GTCCATAAAGAAAAGTACAA+AGG 0.651884 1_4:+26728489 Msa0158130:five_prime_UTR
ACAGCTTTAAGTGATTTAAG+GGG 0.657063 1_4:+26731296 Msa0158130:three_prime_UTR
TTAATTTCACCAATAGCACA+AGG 0.659786 1_4:-26731468 None:intergenic
TTGCTGGAGGAGCTTGAACG+AGG 0.667991 1_4:+26729676 Msa0158130:CDS
GTGAGTGTTGTGGATCTGAT+CGG 0.669660 1_4:-26728634 None:intergenic
TAAAAGTAAGTTCCTTCTGG+AGG 0.670760 1_4:-26730853 None:intergenic
TAATTTCACCAATAGCACAA+GGG 0.679428 1_4:-26731467 None:intergenic
TTGGCAAAGAGAGTACAGCA+TGG 0.708361 1_4:+26730768 Msa0158130:CDS
TGTTAACCATGAAACTGGCG+TGG 0.731270 1_4:+26730543 Msa0158130:CDS
GCAAAGAGAGTACAGCATGG+AGG 0.733074 1_4:+26730771 Msa0158130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAATAGAAAAAGAAAA+TGG - chr1_4:26728555-26728574 None:intergenic 15.0%
!! TAATTGAGTAAAAAAGAAAA+CGG - chr1_4:26728735-26728754 None:intergenic 15.0%
!!! TATGTAATTATCGTTAATTT+TGG + chr1_4:26728859-26728878 Msa0158130:intron 15.0%
!!! ATGTAATTATCGTTAATTTT+GGG + chr1_4:26728860-26728879 Msa0158130:intron 15.0%
!!! TTTTTTTTTTTTTTTACCGA+TGG + chr1_4:26728883-26728902 Msa0158130:intron 15.0%
!! TTTAAAGAAATAGAAATCAA+TGG + chr1_4:26729186-26729205 Msa0158130:intron 15.0%
!!! TGATACTTTAAAAAGAAAAA+TGG + chr1_4:26729347-26729366 Msa0158130:intron 15.0%
!! TAAAAAGAAAAATGGTAAAA+TGG + chr1_4:26729355-26729374 Msa0158130:intron 15.0%
!!! GTTAAGATTTTCTTGAAATT+TGG - chr1_4:26728521-26728540 None:intergenic 20.0%
!! CTGTAATTGAATTATAATTC+TGG + chr1_4:26729411-26729430 Msa0158130:intron 20.0%
!! ATATGAAATATTGTTGTATC+TGG + chr1_4:26730298-26730317 Msa0158130:intron 20.0%
!! AATCAAGATTTATATGAAAC+AGG + chr1_4:26730714-26730733 Msa0158130:intron 20.0%
!! GATTTTCTTGAAATTTGGAA+TGG - chr1_4:26728516-26728535 None:intergenic 25.0%
! AAAACTACATCGATGATTTA+AGG - chr1_4:26728830-26728849 None:intergenic 25.0%
!!! TTTTTTTACCGATGGAATTT+TGG + chr1_4:26728891-26728910 Msa0158130:intron 25.0%
!! ATTGAAATGTTCGATCATTT+TGG + chr1_4:26729013-26729032 Msa0158130:intron 25.0%
!!! TTTTTAGTAGTTTCTTGTTG+AGG + chr1_4:26729095-26729114 Msa0158130:intron 25.0%
! AAAGAAAAATGGTAAAATGG+TGG + chr1_4:26729358-26729377 Msa0158130:intron 25.0%
! AAGAAAAATGGTAAAATGGT+GGG + chr1_4:26729359-26729378 Msa0158130:intron 25.0%
! GATATGTACAAAATATTAGC+CGG - chr1_4:26729449-26729468 None:intergenic 25.0%
! GAGAAATACTTACATTATGA+GGG - chr1_4:26729792-26729811 None:intergenic 25.0%
! AGAGAAATACTTACATTATG+AGG - chr1_4:26729793-26729812 None:intergenic 25.0%
!!! TAAACTGGTTCTATCAAATT+AGG - chr1_4:26729909-26729928 None:intergenic 25.0%
! ATATAATGTGTAATGCTTTG+TGG + chr1_4:26730927-26730946 Msa0158130:three_prime_UTR 25.0%
!!! TATGTTTGCACAATTGATTT+TGG + chr1_4:26731238-26731257 Msa0158130:three_prime_UTR 25.0%
!!! ATGTTTGCACAATTGATTTT+GGG + chr1_4:26731239-26731258 Msa0158130:three_prime_UTR 25.0%
!!! TACAGCTTTAAGTGATTTAA+GGG + chr1_4:26731295-26731314 Msa0158130:three_prime_UTR 25.0%
! TTGTACTTTTCTTTATGGAC+CGG - chr1_4:26728489-26728508 None:intergenic 30.0%
! GTCCATAAAGAAAAGTACAA+AGG + chr1_4:26728489-26728508 Msa0158130:five_prime_UTR 30.0%
! GTCCTTTGTACTTTTCTTTA+TGG - chr1_4:26728494-26728513 None:intergenic 30.0%
TGTGGAGAATAAACAAAGAA+GGG - chr1_4:26728578-26728597 None:intergenic 30.0%
TTGTGGAGAATAAACAAAGA+AGG - chr1_4:26728579-26728598 None:intergenic 30.0%
ATTACAGAAAACTTGCACAA+AGG - chr1_4:26729398-26729417 None:intergenic 30.0%
ATATTACGAACACCTAACAA+TGG - chr1_4:26729831-26729850 None:intergenic 30.0%
TTTGATAGAACCAGTTTATG+TGG + chr1_4:26729911-26729930 Msa0158130:intron 30.0%
! CGCATAAACTACAATTTTCA+AGG - chr1_4:26730002-26730021 None:intergenic 30.0%
CATGAACACTTCTTACTTAT+TGG + chr1_4:26730191-26730210 Msa0158130:intron 30.0%
TATCTGGAAATCTGAAAGTT+CGG + chr1_4:26730314-26730333 Msa0158130:intron 30.0%
! TAATTTTGTGCGTACTTAAC+AGG + chr1_4:26730371-26730390 Msa0158130:intron 30.0%
GATTACATCTCTGATAACAA+TGG - chr1_4:26730698-26730717 None:intergenic 30.0%
AGGTTGAACCAAAGAAATTT+GGG + chr1_4:26730734-26730753 Msa0158130:intron 30.0%
AACATGTTCTTGAATTCAGA+AGG - chr1_4:26730955-26730974 None:intergenic 30.0%
TTCTGAATTCAAGAACATGT+TGG + chr1_4:26730954-26730973 Msa0158130:three_prime_UTR 30.0%
TCTGAATTCAAGAACATGTT+GGG + chr1_4:26730955-26730974 Msa0158130:three_prime_UTR 30.0%
TTCCTTCAGATTACATACAA+TGG + chr1_4:26731110-26731129 Msa0158130:three_prime_UTR 30.0%
TACCATTGTATGTAATCTGA+AGG - chr1_4:26731115-26731134 None:intergenic 30.0%
!! TGTTTGCACAATTGATTTTG+GGG + chr1_4:26731240-26731259 Msa0158130:three_prime_UTR 30.0%
CTTAAATCACTTAAAGCTGT+AGG - chr1_4:26731296-26731315 None:intergenic 30.0%
!! CTACAGCTTTAAGTGATTTA+AGG + chr1_4:26731294-26731313 Msa0158130:three_prime_UTR 30.0%
!! ACAGCTTTAAGTGATTTAAG+GGG + chr1_4:26731296-26731315 Msa0158130:three_prime_UTR 30.0%
! TCTTGTTCGTTGGTAAAAAT+TGG + chr1_4:26731321-26731340 Msa0158130:three_prime_UTR 30.0%
!! TGTGACTGATTTGTTGAATA+TGG + chr1_4:26731349-26731368 Msa0158130:three_prime_UTR 30.0%
! ATTTGTTGAATATGGACATG+TGG + chr1_4:26731357-26731376 Msa0158130:three_prime_UTR 30.0%
TAATTTCACCAATAGCACAA+GGG - chr1_4:26731470-26731489 None:intergenic 30.0%
TTAATTTCACCAATAGCACA+AGG - chr1_4:26731471-26731490 None:intergenic 30.0%
!! AAAGAAAATGGAGTGCTGAT+CGG - chr1_4:26728543-26728562 None:intergenic 35.0%
!! TTTTGTTGAAGTGAGTGTTG+TGG - chr1_4:26728647-26728666 None:intergenic 35.0%
! CATCGATGATTTAAGGTCAA+CGG - chr1_4:26728823-26728842 None:intergenic 35.0%
! TTACCGATGGAATTTTGGAT+CGG + chr1_4:26728896-26728915 Msa0158130:intron 35.0%
! ATTCTGGTTGTTCTTGTTTC+CGG + chr1_4:26729427-26729446 Msa0158130:intron 35.0%
CATATCATGTGTTTATCCTG+AGG + chr1_4:26729463-26729482 Msa0158130:intron 35.0%
TTTCAAATATGTACTGCCTC+AGG - chr1_4:26729482-26729501 None:intergenic 35.0%
TTGATGTCTTGTGACTGTAA+AGG + chr1_4:26729511-26729530 Msa0158130:intron 35.0%
TGATGTCTTGTGACTGTAAA+GGG + chr1_4:26729512-26729531 Msa0158130:intron 35.0%
TATCCAACTTCCACATAAAC+TGG - chr1_4:26729924-26729943 None:intergenic 35.0%
!! TTTATGCGAAGTGTTTTCAC+CGG + chr1_4:26730014-26730033 Msa0158130:intron 35.0%
CAAGAACTCAAAAAACATGC+CGG - chr1_4:26730036-26730055 None:intergenic 35.0%
TACTTTCAGACTGTACATGA+AGG + chr1_4:26730430-26730449 Msa0158130:intron 35.0%
ACTTGTGTTAACCATGAAAC+TGG + chr1_4:26730538-26730557 Msa0158130:CDS 35.0%
! GAAGATGATATGAAAGCATG+TGG - chr1_4:26730584-26730603 None:intergenic 35.0%
!! AACACAATCTCTCTGCTTTT+TGG + chr1_4:26730658-26730677 Msa0158130:intron 35.0%
! CTCTGATAACAATGGATTCA+AGG - chr1_4:26730690-26730709 None:intergenic 35.0%
! CAGGTTGAACCAAAGAAATT+TGG + chr1_4:26730733-26730752 Msa0158130:intron 35.0%
AATTTGGGCTTCTTGCAAAT+TGG + chr1_4:26730749-26730768 Msa0158130:CDS 35.0%
TAAAAGTAAGTTCCTTCTGG+AGG - chr1_4:26730856-26730875 None:intergenic 35.0%
TGCTAAAAGTAAGTTCCTTC+TGG - chr1_4:26730859-26730878 None:intergenic 35.0%
ATCCAAGATCGTATAGTTGT+TGG + chr1_4:26730878-26730897 Msa0158130:three_prime_UTR 35.0%
ATCCAACAACTATACGATCT+TGG - chr1_4:26730883-26730902 None:intergenic 35.0%
CTGAATTCAAGAACATGTTG+GGG + chr1_4:26730956-26730975 Msa0158130:three_prime_UTR 35.0%
GCAGAAGAAAGTAAAGCAAA+AGG - chr1_4:26731159-26731178 None:intergenic 35.0%
AATCCAAATCCGCAATAAAC+AGG - chr1_4:26731266-26731285 None:intergenic 35.0%
! GTTCGTTGGTAAAAATTGGA+TGG + chr1_4:26731325-26731344 Msa0158130:three_prime_UTR 35.0%
GAAAAGAAGAACGCGAATTG+TGG - chr1_4:26728596-26728615 None:intergenic 40.0%
AAAACGGCGAAGAGTTAGAT+TGG - chr1_4:26728719-26728738 None:intergenic 40.0%
TCACCGATCCAAAATTCCAT+CGG - chr1_4:26728902-26728921 None:intergenic 40.0%
ATGGATGTTACTGCATGTGT+CGG + chr1_4:26729205-26729224 Msa0158130:intron 40.0%
!! GTGTAGTTGGTGTTAGGATT+CGG + chr1_4:26729227-26729246 Msa0158130:intron 40.0%
TATCCGTACTTCAGAGTTTC+CGG + chr1_4:26729294-26729313 Msa0158130:intron 40.0%
! GAACGAGGTGAAAAAGGTAT+TGG + chr1_4:26729691-26729710 Msa0158130:CDS 40.0%
!! GGTGAAAAAGGTATTGGTGA+TGG + chr1_4:26729697-26729716 Msa0158130:CDS 40.0%
GTTAGCTATGGAATGGATGA+TGG + chr1_4:26729724-26729743 Msa0158130:CDS 40.0%
ATGACATTTACATGCGCTCT+TGG + chr1_4:26729749-26729768 Msa0158130:CDS 40.0%
TACATTATGAGGGCCAATGA+TGG - chr1_4:26729782-26729801 None:intergenic 40.0%
ACTTCTCTGCATCCATTGTT+AGG + chr1_4:26729816-26729835 Msa0158130:intron 40.0%
GAACCAGTTTATGTGGAAGT+TGG + chr1_4:26729918-26729937 Msa0158130:intron 40.0%
! TATGTTCTGTTGCTTCAAGC+TGG - chr1_4:26729964-26729983 None:intergenic 40.0%
CTGTATTTGCACATGAACAC+TGG + chr1_4:26730099-26730118 Msa0158130:intron 40.0%
! TTTTGTGCGTACTTAACAGG+TGG + chr1_4:26730374-26730393 Msa0158130:intron 40.0%
! GTACTTAACAGGTGGTTTTC+AGG + chr1_4:26730382-26730401 Msa0158130:intron 40.0%
GCAAGAAGCCCAAATTTCTT+TGG - chr1_4:26730745-26730764 None:intergenic 40.0%
! TGCCATTTCTTTCTTCAGCT+GGG - chr1_4:26730806-26730825 None:intergenic 40.0%
TGCTCCTATTTCCATCTCTA+TGG + chr1_4:26730981-26731000 Msa0158130:three_prime_UTR 40.0%
GAAGCCATAGAGATGGAAAT+AGG - chr1_4:26730988-26731007 None:intergenic 40.0%
TACTTTGTCAACTGTGCTGA+AGG + chr1_4:26731026-26731045 Msa0158130:three_prime_UTR 40.0%
ACTTTGTCAACTGTGCTGAA+GGG + chr1_4:26731027-26731046 Msa0158130:three_prime_UTR 40.0%
TTAGCTCCAGCATACTTACA+AGG - chr1_4:26731181-26731200 None:intergenic 40.0%
GTAAGTATGCTGGAGCTAAA+TGG + chr1_4:26731182-26731201 Msa0158130:three_prime_UTR 40.0%
!!! ATTTTGGGGCCTGTTTATTG+CGG + chr1_4:26731254-26731273 Msa0158130:three_prime_UTR 40.0%
CACTTAAAGCTGTAGGTGTA+GGG - chr1_4:26731289-26731308 None:intergenic 40.0%
TCACTTAAAGCTGTAGGTGT+AGG - chr1_4:26731290-26731309 None:intergenic 40.0%
! CACAAGGGATTTTGCCATAA+TGG - chr1_4:26731455-26731474 None:intergenic 40.0%
! GTGAGTGTTGTGGATCTGAT+CGG - chr1_4:26728637-26728656 None:intergenic 45.0%
CAAAATCCATCCATGACGCT+TGG + chr1_4:26728662-26728681 Msa0158130:exon 45.0%
ATGCGTGTCAGACACTTTGA+CGG + chr1_4:26729256-26729275 Msa0158130:intron 45.0%
!! TAGCCGGAAACTCTGAAGTA+CGG - chr1_4:26729300-26729319 None:intergenic 45.0%
CATCACACTTATCTCGTAGC+CGG - chr1_4:26729316-26729335 None:intergenic 45.0%
GCTACGAGATAAGTGTGATG+AGG + chr1_4:26729316-26729335 Msa0158130:intron 45.0%
TCCAAGGAACTTCAGATTGC+TGG + chr1_4:26729660-26729679 Msa0158130:CDS 45.0%
! TCCAGCAATCTGAAGTTCCT+TGG - chr1_4:26729664-26729683 None:intergenic 45.0%
AAGGAACTTCAGATTGCTGG+AGG + chr1_4:26729663-26729682 Msa0158130:CDS 45.0%
GAGCTTGAACGAGGTGAAAA+AGG + chr1_4:26729685-26729704 Msa0158130:CDS 45.0%
TGGCACTGTTAGCTATGGAA+TGG + chr1_4:26729717-26729736 Msa0158130:CDS 45.0%
ATTTACATGCGCTCTTGGAC+TGG + chr1_4:26729754-26729773 Msa0158130:CDS 45.0%
GCTCACAAGCAACTAAACAC+AGG - chr1_4:26730071-26730090 None:intergenic 45.0%
CATATGCCCTTCCTATGTTC+TGG + chr1_4:26730234-26730253 Msa0158130:intron 45.0%
GAATGAAACCGAACACTTGG+CGG - chr1_4:26730505-26730524 None:intergenic 45.0%
CGTGAATGAAACCGAACACT+TGG - chr1_4:26730508-26730527 None:intergenic 45.0%
TGTTAACCATGAAACTGGCG+TGG + chr1_4:26730543-26730562 Msa0158130:CDS 45.0%
! TTGGCAAAGAGAGTACAGCA+TGG + chr1_4:26730768-26730787 Msa0158130:CDS 45.0%
GACCCAGCTGAAGAAAGAAA+TGG + chr1_4:26730801-26730820 Msa0158130:CDS 45.0%
! CTGCCATTTCTTTCTTCAGC+TGG - chr1_4:26730807-26730826 None:intergenic 45.0%
!! CAACTCTGAAGCCATAGAGA+TGG - chr1_4:26730995-26731014 None:intergenic 45.0%
CTTCTGCCTTGTAAGTATGC+TGG + chr1_4:26731172-26731191 Msa0158130:three_prime_UTR 45.0%
!! TTAAGGGGTGTCTTGTTCGT+TGG + chr1_4:26731311-26731330 Msa0158130:three_prime_UTR 45.0%
!! TGCTGATTTGCTTGACTGCA+GGG + chr1_4:26731386-26731405 Msa0158130:three_prime_UTR 45.0%
CACCAGTGAGATTGCCATTA+TGG + chr1_4:26731438-26731457 Msa0158130:three_prime_UTR 45.0%
TGCCATAATGGCAATCTCAC+TGG - chr1_4:26731443-26731462 None:intergenic 45.0%
GGCAAAATCCCTTGTGCTAT+TGG + chr1_4:26731459-26731478 Msa0158130:three_prime_UTR 45.0%
TGGTAACTAACCGACGACAC+TGG - chr1_4:26728699-26728718 None:intergenic 50.0%
! ACTGCATGTGTCGGTGTAGT+TGG + chr1_4:26729214-26729233 Msa0158130:intron 50.0%
! GTGTCGGTGTAGTTGGTGTT+AGG + chr1_4:26729221-26729240 Msa0158130:intron 50.0%
TGCGCTTCAATGCAGTTCCA+AGG + chr1_4:26729644-26729663 Msa0158130:intron 50.0%
! GGTGATGGCACTGTTAGCTA+TGG + chr1_4:26729712-26729731 Msa0158130:CDS 50.0%
!! TCTTGGACTGGCACCATCAT+TGG + chr1_4:26729766-26729785 Msa0158130:CDS 50.0%
ATGCCCTTCCTATGTTCTGG+CGG + chr1_4:26730237-26730256 Msa0158130:intron 50.0%
GAAAAGACCCGCCAGAACAT+AGG - chr1_4:26730248-26730267 None:intergenic 50.0%
! GAACACTTGGCGGCTTTTCT+GGG - chr1_4:26730495-26730514 None:intergenic 50.0%
CAGAAAAGCCGCCAAGTGTT+CGG + chr1_4:26730494-26730513 Msa0158130:CDS 50.0%
CTGATACCACGCCAGTTTCA+TGG - chr1_4:26730552-26730571 None:intergenic 50.0%
! GCAAAGAGAGTACAGCATGG+AGG + chr1_4:26730771-26730790 Msa0158130:CDS 50.0%
AAATGGCAGCCCCTCATAAC+CGG + chr1_4:26730818-26730837 Msa0158130:CDS 50.0%
GACAAGCTTCCGGTTATGAG+GGG - chr1_4:26730830-26730849 None:intergenic 50.0%
GGACAAGCTTCCGGTTATGA+GGG - chr1_4:26730831-26730850 None:intergenic 50.0%
TGGACAAGCTTCCGGTTATG+AGG - chr1_4:26730832-26730851 None:intergenic 50.0%
! AGTAAGTTCCTTCTGGAGGC+TGG - chr1_4:26730852-26730871 None:intergenic 50.0%
GGGCCTGTTTATTGCGGATT+TGG + chr1_4:26731260-26731279 Msa0158130:three_prime_UTR 50.0%
!! GTGCTGATTTGCTTGACTGC+AGG + chr1_4:26731385-26731404 Msa0158130:three_prime_UTR 50.0%
CTAACCGACGACACTGGATC+CGG - chr1_4:26728693-26728712 None:intergenic 55.0%
TTGCTGGAGGAGCTTGAACG+AGG + chr1_4:26729676-26729695 Msa0158130:CDS 55.0%
TGCCCTTCCTATGTTCTGGC+GGG + chr1_4:26730238-26730257 Msa0158130:intron 55.0%
GACCCGCCAGAACATAGGAA+GGG - chr1_4:26730243-26730262 None:intergenic 55.0%
AGACCCGCCAGAACATAGGA+AGG - chr1_4:26730244-26730263 None:intergenic 55.0%
! CGAACACTTGGCGGCTTTTC+TGG - chr1_4:26730496-26730515 None:intergenic 55.0%
AAGCTTGTCCAGCCTCCAGA+AGG + chr1_4:26730841-26730860 Msa0158130:CDS 55.0%
GCTGAGCCAAGCGTCATGGA+TGG - chr1_4:26728671-26728690 None:intergenic 60.0%
TCCATGACGCTTGGCTCAGC+CGG + chr1_4:26728671-26728690 Msa0158130:exon 60.0%
TCAGCCGGATCCAGTGTCGT+CGG + chr1_4:26728686-26728705 Msa0158130:CDS 60.0%
CTGGAGGCTGGACAAGCTTC+CGG - chr1_4:26730840-26730859 None:intergenic 60.0%
TCCGGCTGAGCCAAGCGTCA+TGG - chr1_4:26728675-26728694 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 26728484 26731503 26728484 ID=Msa0158130;Name=Msa0158130
chr1_4 mRNA 26728484 26731503 26728484 ID=Msa0158130-mRNA-1;Parent=Msa0158130;Name=Msa0158130-mRNA-1;_AED=0.07;_eAED=0.07;_QI=190|1|1|1|1|1|4|627|146
chr1_4 exon 26728484 26728707 26728484 ID=Msa0158130-mRNA-1:exon:8849;Parent=Msa0158130-mRNA-1
chr1_4 exon 26729659 26729798 26729659 ID=Msa0158130-mRNA-1:exon:8850;Parent=Msa0158130-mRNA-1
chr1_4 exon 26730439 26730564 26730439 ID=Msa0158130-mRNA-1:exon:8851;Parent=Msa0158130-mRNA-1
chr1_4 exon 26730736 26731503 26730736 ID=Msa0158130-mRNA-1:exon:8852;Parent=Msa0158130-mRNA-1
chr1_4 five_prime_UTR 26728484 26728673 26728484 ID=Msa0158130-mRNA-1:five_prime_utr;Parent=Msa0158130-mRNA-1
chr1_4 CDS 26728674 26728707 26728674 ID=Msa0158130-mRNA-1:cds;Parent=Msa0158130-mRNA-1
chr1_4 CDS 26729659 26729798 26729659 ID=Msa0158130-mRNA-1:cds;Parent=Msa0158130-mRNA-1
chr1_4 CDS 26730439 26730564 26730439 ID=Msa0158130-mRNA-1:cds;Parent=Msa0158130-mRNA-1
chr1_4 CDS 26730736 26730876 26730736 ID=Msa0158130-mRNA-1:cds;Parent=Msa0158130-mRNA-1
chr1_4 three_prime_UTR 26730877 26731503 26730877 ID=Msa0158130-mRNA-1:three_prime_utr;Parent=Msa0158130-mRNA-1
Gene Sequence

>Msa0158130

ATGACGCTTGGCTCAGCCGGATCCAGTGTCGTCGTTCCAAGGAACTTCAGATTGCTGGAGGAGCTTGAACGAGGTGAAAAAGGTATTGGTGATGGCACTGTTAGCTATGGAATGGATGATGGTGATGACATTTACATGCGCTCTTGGACTGGCACCATCATTGGCCCTCATAATACTGTACATGAAGGCAGAATATATCAATTGAAGCTGTTTTGTGACAAAGATTACCCAGAAAAGCCGCCAAGTGTTCGGTTTCATTCACGAATCAATATGACTTGTGTTAACCATGAAACTGGCGTGGTTGAACCAAAGAAATTTGGGCTTCTTGCAAATTGGCAAAGAGAGTACAGCATGGAGGATATACTGACCCAGCTGAAGAAAGAAATGGCAGCCCCTCATAACCGGAAGCTTGTCCAGCCTCCAGAAGGAACTTACTTTTAG
Protein sequence

>Msa0158130

MTLGSAGSSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYSMEDILTQLKKEMAAPHNRKLVQPPEGTYF*