AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0506190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0506190 MtrunA17_Chr3g0107461 89.441 161 17 0 1 161 1 161 1.03e-106 301
Msa0506190 MtrunA17_Chr4g0031421 78.947 133 28 0 29 161 1 133 3.81e-73 216
Msa0506190 MtrunA17_Chr3g0105791 78.195 133 29 0 29 161 1 133 2.65e-72 214
Msa0506190 MtrunA17_Chr3g0107381 54.839 155 67 2 1 154 1 153 5.22e-55 172
Msa0506190 MtrunA17_Chr3g0110131 49.032 155 78 1 1 155 1 154 2.25e-41 138
Msa0506190 MtrunA17_Chr2g0296861 47.097 155 81 1 1 155 1 154 8.95e-41 134
Msa0506190 MtrunA17_Chr2g0283941 42.308 156 90 0 5 160 5 160 6.43e-40 134
Msa0506190 MtrunA17_Chr5g0432691 39.610 154 92 1 1 154 1 153 1.54e-37 126
Msa0506190 MtrunA17_Chr8g0351751 41.509 159 91 1 1 157 1 159 1.35e-36 126
Msa0506190 MtrunA17_Chr4g0014321 37.662 154 96 0 1 154 1 154 6.85e-36 124
Msa0506190 MtrunA17_Chr3g0107331 40.397 151 89 1 3 153 2 151 1.43e-33 116
Msa0506190 MtrunA17_Chr3g0106421 44.697 132 73 0 29 160 1 132 1.76e-33 117
Msa0506190 MtrunA17_Chr4g0014031 42.949 156 89 0 1 156 1 156 6.01e-33 116
Msa0506190 MtrunA17_Chr4g0014131 42.857 161 92 0 1 161 1 161 7.26e-33 116
Msa0506190 MtrunA17_Chr2g0296831 51.282 117 57 0 1 117 1 117 1.35e-32 112
Msa0506190 MtrunA17_Chr3g0106501 44.961 129 71 0 29 157 1 129 2.46e-31 110
Msa0506190 MtrunA17_Chr4g0014121 39.103 156 95 0 1 156 29 184 3.51e-30 108
Msa0506190 MtrunA17_Chr4g0013041 35.714 154 97 1 1 154 1 152 1.02e-29 107
Msa0506190 MtrunA17_Chr5g0420351 37.580 157 96 2 1 157 1 155 1.62e-29 105
Msa0506190 MtrunA17_Chr3g0107351 36.129 155 98 1 3 157 2 155 1.73e-29 105
Msa0506190 MtrunA17_Chr3g0107341 36.129 155 98 1 3 157 2 155 1.83e-27 100
Msa0506190 MtrunA17_Chr3g0107361 36.129 155 98 1 3 157 2 155 1.95e-27 100
Msa0506190 MtrunA17_Chr1g0188681 38.750 160 90 4 1 157 1 155 3.01e-27 100
Msa0506190 MtrunA17_Chr1g0197461 34.194 155 100 2 3 157 2 154 1.45e-26 98.2
Msa0506190 MtrunA17_Chr4g0012761 36.943 157 97 2 1 157 1 155 1.37e-24 93.2
Msa0506190 MtrunA17_Chr1g0188661 36.709 158 96 3 1 157 1 155 1.47e-24 93.2
Msa0506190 MtrunA17_Chr1g0197261 32.278 158 103 3 1 157 1 155 2.54e-23 89.7
Msa0506190 MtrunA17_Chr7g0218151 35.032 157 100 2 1 157 1 155 5.93e-23 89.0
Msa0506190 MtrunA17_Chr1g0188651 35.404 161 100 2 1 161 1 157 1.62e-22 87.8
Msa0506190 MtrunA17_Chr1g0197321 32.075 159 103 3 1 157 1 156 2.41e-22 87.4
Msa0506190 MtrunA17_Chr1g0193311 35.443 158 98 3 1 157 1 155 2.88e-22 87.0
Msa0506190 MtrunA17_Chr4g0008701 28.148 135 93 1 16 150 19 149 5.33e-14 68.6
Msa0506190 MtrunA17_Chr7g0267601 28.070 114 82 0 5 118 8 121 1.18e-12 64.7
Msa0506190 MtrunA17_Chr3g0128721 26.056 142 96 2 16 153 19 155 5.59e-12 62.8
Msa0506190 MtrunA17_Chr6g0457851 29.204 113 73 3 1 107 11 122 1.09e-11 61.6
Msa0506190 MtrunA17_Chr1g0212151 28.448 116 76 3 1 110 1 115 2.86e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0506190 AT5G48670.1 48.718 156 77 2 1 155 1 154 1.08e-37 131
Msa0506190 AT3G05860.2 46.281 121 61 1 1 117 1 121 4.47e-30 108
Msa0506190 AT3G05860.3 46.281 121 61 1 1 117 1 121 6.78e-30 109
Msa0506190 AT3G05860.1 46.281 121 61 1 1 117 1 121 1.15e-29 109
Msa0506190 AT1G65330.1 40.541 148 87 1 3 150 2 148 2.19e-29 108
Msa0506190 AT1G65300.1 39.490 157 93 2 5 161 4 158 2.57e-29 108
Msa0506190 AT5G27810.1 52.809 89 42 0 29 117 1 89 1.64e-28 102
Msa0506190 AT5G26650.1 35.948 153 97 1 5 157 3 154 5.45e-25 99.0
Msa0506190 AT5G27960.1 34.641 153 99 1 5 157 3 154 8.74e-24 95.1
Msa0506190 AT1G22590.2 33.125 160 90 2 1 157 1 146 8.47e-23 89.0
Msa0506190 AT1G31640.1 38.994 159 83 4 3 155 2 152 1.75e-22 92.8
Msa0506190 AT1G31630.1 35.185 162 95 5 3 161 2 156 1.84e-22 91.7
Msa0506190 AT2G28700.1 32.667 150 100 1 1 150 1 149 8.16e-21 87.0
Msa0506190 AT5G26630.1 33.548 155 102 1 1 155 1 154 2.22e-20 84.0
Msa0506190 AT5G06500.1 33.333 147 88 3 1 147 1 137 3.96e-18 78.6
Msa0506190 AT2G40210.1 33.663 101 67 0 1 101 1 101 1.03e-13 67.8
Msa0506190 AT5G26580.1 36.634 101 50 1 1 101 1 87 3.91e-13 66.2
Msa0506190 AT3G57230.1 29.012 162 100 4 1 161 1 148 1.07e-12 63.9
Msa0506190 AT3G57230.4 29.012 162 100 4 1 161 1 148 1.07e-12 63.9
Msa0506190 AT3G57230.2 29.825 114 76 2 1 113 1 111 3.04e-11 58.5

Find 36 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCTGCTTTC+TGG 0.066665 3_4:+52399367 None:intergenic
ATTCATACCACAGAATTTAA+TGG 0.196701 3_4:-52399329 Msa0506190:CDS
CAGGTTTGGCCATCAGATTC+AGG 0.280803 3_4:-52399524 Msa0506190:CDS
CTGAATGTTATCCTCCTCTT+CGG 0.350265 3_4:+52399839 None:intergenic
GTTCAGGAGTTTGCCTGGAC+TGG 0.382826 3_4:-52399480 Msa0506190:CDS
CTGAATCTGATGGCCAAACC+TGG 0.394292 3_4:+52399525 None:intergenic
TGAATGTTATCCTCCTCTTC+GGG 0.395416 3_4:+52399840 None:intergenic
GAAGTGTGATATCCAGGTTC+AGG 0.406864 3_4:-52399496 Msa0506190:CDS
GATGAAATTAGCACTCTTTG+CGG 0.413383 3_4:-52399593 Msa0506190:CDS
AATGAAATGATGAATGATAT+TGG 0.442815 3_4:+52399349 None:intergenic
TCCAGGTTCAGGAGTTTGCC+TGG 0.447666 3_4:-52399485 Msa0506190:CDS
ACAATCCTCAAGCCCAGGTT+TGG 0.451431 3_4:-52399538 Msa0506190:CDS
TATTCTTGAGGCAAAGCATT+GGG 0.452810 3_4:-52399427 Msa0506190:CDS
GAGGACAAATCATTAAGATC+AGG 0.474701 3_4:+52399290 None:intergenic
GTGTTAGAAGTGTGATATCC+AGG 0.495852 3_4:-52399502 Msa0506190:CDS
CACTATCATGGCTAGAGGTA+AGG 0.508320 3_4:-52399888 None:intergenic
TCCAGGCAAACTCCTGAACC+TGG 0.517673 3_4:+52399484 None:intergenic
AAGGAAGGAAACCAGAAAGC+AGG 0.520416 3_4:-52399378 Msa0506190:CDS
CAAATTCTCAGCAATAAAAG+AGG 0.520681 3_4:+52399271 None:intergenic
GGAGGATAACATTCAGAAAG+AGG 0.525464 3_4:-52399832 Msa0506190:CDS
TAAATATCCATTAAATTCTG+TGG 0.554522 3_4:+52399322 None:intergenic
GTATTCTTGAGGCAAAGCAT+TGG 0.555152 3_4:-52399428 Msa0506190:CDS
GATGGCCAAACCTGGGCTTG+AGG 0.558655 3_4:+52399533 None:intergenic
TTGCTCGTAAATTATTGCAC+CGG 0.562136 3_4:+52399562 None:intergenic
GCAAAACAATCCTCAAGCCC+AGG 0.573782 3_4:-52399543 Msa0506190:CDS
TGAATCTGATGGCCAAACCT+GGG 0.601638 3_4:+52399526 None:intergenic
GAGGTCGATCAAAAGATGAA+AGG 0.605953 3_4:-52399245 Msa0506190:CDS
ATGAGCAGATGATGAAACAA+AGG 0.611511 3_4:-52399397 Msa0506190:CDS
ACTCTTTGCGGAATAGAAGC+CGG 0.612299 3_4:-52399581 Msa0506190:CDS
CTTCTAACACCTGAATCTGA+TGG 0.613550 3_4:+52399515 None:intergenic
ACATAACATGTGACCCGAAG+AGG 0.618667 3_4:-52399853 Msa0506190:CDS
TATTGCTGAGAATTTGAGGG+AGG 0.639350 3_4:-52399264 Msa0506190:CDS
GCAGATGATGAAACAAAGGA+AGG 0.642572 3_4:-52399393 Msa0506190:CDS
ATCCTCACTATCATGGCTAG+AGG 0.659018 3_4:-52399893 None:intergenic
TAACATGTGACCCGAAGAGG+AGG 0.659370 3_4:-52399850 Msa0506190:CDS
TGGATCAAGAGGTATTCTTG+AGG 0.709854 3_4:-52399439 Msa0506190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATGGATTTTTTTTATTA+GGG - chr3_4:52399476-52399495 Msa0506190:CDS 10.0%
!!! TAAATTTGGAATTTTACTAT+AGG - chr3_4:52399443-52399462 Msa0506190:CDS 15.0%
!! TATAGGTTATATATTGTTTA+TGG - chr3_4:52399460-52399479 Msa0506190:CDS 15.0%
!!! GTTTATGGATTTTTTTTATT+AGG - chr3_4:52399475-52399494 Msa0506190:CDS 15.0%
!! AATGAAATGATGAATGATAT+TGG + chr3_4:52399761-52399780 None:intergenic 20.0%
!! TAAATATCCATTAAATTCTG+TGG + chr3_4:52399788-52399807 None:intergenic 20.0%
! ATTCATACCACAGAATTTAA+TGG - chr3_4:52399778-52399797 Msa0506190:intron 25.0%
!! TTTTATTGCTGAGAATTTGA+GGG - chr3_4:52399840-52399859 Msa0506190:CDS 25.0%
ACATTCAGAAAGAGGAAAAA+TGG - chr3_4:52399283-52399302 Msa0506190:CDS 30.0%
TATGTATGATATGCAAAGAG+AGG + chr3_4:52399319-52399338 None:intergenic 30.0%
ACTGGAACAAAGTAAAAAGA+TGG - chr3_4:52399645-52399664 Msa0506190:intron 30.0%
CAAATTCTCAGCAATAAAAG+AGG + chr3_4:52399839-52399858 None:intergenic 30.0%
! CTTTTATTGCTGAGAATTTG+AGG - chr3_4:52399839-52399858 Msa0506190:CDS 30.0%
GATGAAATTAGCACTCTTTG+CGG - chr3_4:52399514-52399533 Msa0506190:CDS 35.0%
TTGCTCGTAAATTATTGCAC+CGG + chr3_4:52399548-52399567 None:intergenic 35.0%
! TTTTTACTTTGTTCCAGTCC+AGG + chr3_4:52399643-52399662 None:intergenic 35.0%
GGAACAAAGTAAAAAGATGG+TGG - chr3_4:52399648-52399667 Msa0506190:intron 35.0%
!! TAAAAAGATGGTGGATCAAG+AGG - chr3_4:52399657-52399676 Msa0506190:intron 35.0%
! TATTCTTGAGGCAAAGCATT+GGG - chr3_4:52399680-52399699 Msa0506190:intron 35.0%
ATGAGCAGATGATGAAACAA+AGG - chr3_4:52399710-52399729 Msa0506190:intron 35.0%
GAGGACAAATCATTAAGATC+AGG + chr3_4:52399820-52399839 None:intergenic 35.0%
TGAATGTTATCCTCCTCTTC+GGG + chr3_4:52399270-52399289 None:intergenic 40.0%
CTGAATGTTATCCTCCTCTT+CGG + chr3_4:52399271-52399290 None:intergenic 40.0%
GGAGGATAACATTCAGAAAG+AGG - chr3_4:52399275-52399294 Msa0506190:CDS 40.0%
CTTCTAACACCTGAATCTGA+TGG + chr3_4:52399595-52399614 None:intergenic 40.0%
! GTGTTAGAAGTGTGATATCC+AGG - chr3_4:52399605-52399624 Msa0506190:CDS 40.0%
!! TGGATCAAGAGGTATTCTTG+AGG - chr3_4:52399668-52399687 Msa0506190:intron 40.0%
GTATTCTTGAGGCAAAGCAT+TGG - chr3_4:52399679-52399698 Msa0506190:intron 40.0%
GCAGATGATGAAACAAAGGA+AGG - chr3_4:52399714-52399733 Msa0506190:intron 40.0%
ATTGGTCATTTCCTGCTTTC+TGG + chr3_4:52399743-52399762 None:intergenic 40.0%
TATTGCTGAGAATTTGAGGG+AGG - chr3_4:52399843-52399862 Msa0506190:CDS 40.0%
GAGGTCGATCAAAAGATGAA+AGG - chr3_4:52399862-52399881 Msa0506190:CDS 40.0%
ACATAACATGTGACCCGAAG+AGG - chr3_4:52399254-52399273 Msa0506190:CDS 45.0%
ACTCTTTGCGGAATAGAAGC+CGG - chr3_4:52399526-52399545 Msa0506190:CDS 45.0%
TGAATCTGATGGCCAAACCT+GGG + chr3_4:52399584-52399603 None:intergenic 45.0%
GAAGTGTGATATCCAGGTTC+AGG - chr3_4:52399611-52399630 Msa0506190:CDS 45.0%
AAGGAAGGAAACCAGAAAGC+AGG - chr3_4:52399729-52399748 Msa0506190:intron 45.0%
!!! TAACTTTTATTTAATAAATT+TGG - chr3_4:52399429-52399448 Msa0506190:CDS 5.0%
TAACATGTGACCCGAAGAGG+AGG - chr3_4:52399257-52399276 Msa0506190:CDS 50.0%
GCAAAACAATCCTCAAGCCC+AGG - chr3_4:52399564-52399583 Msa0506190:CDS 50.0%
ACAATCCTCAAGCCCAGGTT+TGG - chr3_4:52399569-52399588 Msa0506190:CDS 50.0%
CTGAATCTGATGGCCAAACC+TGG + chr3_4:52399585-52399604 None:intergenic 50.0%
CAGGTTTGGCCATCAGATTC+AGG - chr3_4:52399583-52399602 Msa0506190:CDS 50.0%
TCCAGGTTCAGGAGTTTGCC+TGG - chr3_4:52399622-52399641 Msa0506190:intron 55.0%
TCCAGGCAAACTCCTGAACC+TGG + chr3_4:52399626-52399645 None:intergenic 55.0%
GTTCAGGAGTTTGCCTGGAC+TGG - chr3_4:52399627-52399646 Msa0506190:intron 55.0%
GATGGCCAAACCTGGGCTTG+AGG + chr3_4:52399577-52399596 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 52399226 52399903 52399226 ID=Msa0506190;Name=Msa0506190
chr3_4 mRNA 52399226 52399903 52399226 ID=Msa0506190-mRNA-1;Parent=Msa0506190;Name=Msa0506190-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|161
chr3_4 exon 52399825 52399903 52399825 ID=Msa0506190-mRNA-1:exon:11434;Parent=Msa0506190-mRNA-1
chr3_4 exon 52399226 52399632 52399226 ID=Msa0506190-mRNA-1:exon:11433;Parent=Msa0506190-mRNA-1
chr3_4 CDS 52399825 52399903 52399825 ID=Msa0506190-mRNA-1:cds;Parent=Msa0506190-mRNA-1
chr3_4 CDS 52399226 52399632 52399226 ID=Msa0506190-mRNA-1:cds;Parent=Msa0506190-mRNA-1
Gene Sequence

>Msa0506190

ATGGCTAGAGGTAAGGTGAAACTAGCATACATAACATGTGACCCGAAGAGGAGGATAACATTCAGAAAGAGGAAAAATGGGATTATGAAGAAGATTGATGAAATTAGCACTCTTTGCGGAATAGAAGCCGGTGCAATAATTTACGAGCAAAACAATCCTCAAGCCCAGGTTTGGCCATCAGATTCAGGTGTTAGAAGTGTGATATCCAGGTTCAGGAGTTTGCCTGGACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGGTATTCTTGAGGCAAAGCATTGGGAAAGTCTATGAGCAGATGATGAAACAAAGGAAGGAAACCAGAAAGCAGGAAATGACCAATATCATTCATCATTTCATTCATACCACAGAATTTAATGGATATTTAATGAGCAAACCTGATCTTAATGATTTGTCCTCTTTTATTGCTGAGAATTTGAGGGAGGTCGATCAAAAGATGAAAGGAATGTTAATCGAAGATTGA

Protein sequence

>Msa0506190

MARGKVKLAYITCDPKRRITFRKRKNGIMKKIDEISTLCGIEAGAIIYEQNNPQAQVWPSDSGVRSVISRFRSLPGLEQSKKMVDQEVFLRQSIGKVYEQMMKQRKETRKQEMTNIIHHFIHTTEFNGYLMSKPDLNDLSSFIAENLREVDQKMKGMLIED*