Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0459170 | KEH34477.1 | 88.820 | 161 | 18 | 0 | 1 | 161 | 1 | 161 | 1.86e-100 | 295 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0459170 | sp|Q9FJK3|AGL80_ARATH | 49.359 | 156 | 76 | 2 | 1 | 155 | 1 | 154 | 1.15e-36 | 131 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0459170 | A0A072UY35 | 88.820 | 161 | 18 | 0 | 1 | 161 | 1 | 161 | 8.90e-101 | 295 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0459170 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016570 | Msa0459170 | 0.818347 | 2.055287e-52 | -8.615850e-47 |
| Msa0102070 | Msa0459170 | 0.847840 | 8.964585e-60 | -8.615850e-47 |
| Msa0111510 | Msa0459170 | 0.820007 | 8.610086e-53 | -8.615850e-47 |
| Msa0142220 | Msa0459170 | 0.829868 | 4.044874e-55 | -8.615850e-47 |
| Msa0145750 | Msa0459170 | 0.805786 | 1.128482e-49 | -8.615850e-47 |
| Msa0200000 | Msa0459170 | 0.812271 | 4.610204e-51 | -8.615850e-47 |
| Msa0227480 | Msa0459170 | 0.835131 | 2.005352e-56 | -8.615850e-47 |
| Msa0231000 | Msa0459170 | 0.841786 | 3.847649e-58 | -8.615850e-47 |
| Msa0235140 | Msa0459170 | 0.835682 | 1.455931e-56 | -8.615850e-47 |
| Msa0365500 | Msa0459170 | 0.816002 | 6.920698e-52 | -8.615850e-47 |
| Msa0366420 | Msa0459170 | 0.801206 | 1.004334e-48 | -8.615850e-47 |
| Msa0384800 | Msa0459170 | 0.871742 | 5.419842e-67 | -8.615850e-47 |
| Msa0384890 | Msa0459170 | 0.804690 | 1.913597e-49 | -8.615850e-47 |
| Msa0406120 | Msa0459170 | 0.804154 | 2.475464e-49 | -8.615850e-47 |
| Msa0414410 | Msa0459170 | 0.859400 | 4.245001e-63 | -8.615850e-47 |
| Msa0414420 | Msa0459170 | 0.884675 | 1.565743e-71 | -8.615850e-47 |
| Msa0436780 | Msa0459170 | 0.801390 | 9.211112e-49 | -8.615850e-47 |
| Msa0436800 | Msa0459170 | 0.806330 | 8.668729e-50 | -8.615850e-47 |
| Msa0438620 | Msa0459170 | 0.832285 | 1.031628e-55 | -8.615850e-47 |
| Msa0450000 | Msa0459170 | 0.836869 | 7.266880e-57 | -8.615850e-47 |
| Msa0452900 | Msa0459170 | 0.869817 | 2.330613e-66 | -8.615850e-47 |
| Msa0453120 | Msa0459170 | 0.823022 | 1.732044e-53 | -8.615850e-47 |
| Msa0459160 | Msa0459170 | 0.832546 | 8.887120e-56 | -8.615850e-47 |
| Msa0459170 | Msa0491590 | 0.808382 | 3.182795e-50 | -8.615850e-47 |
| Msa0459170 | Msa0506190 | 0.818558 | 1.841552e-52 | -8.615850e-47 |
| Msa0459170 | Msa0506270 | 0.811637 | 6.338819e-51 | -8.615850e-47 |
| Msa0459170 | Msa0534540 | 0.811492 | 6.815773e-51 | -8.615850e-47 |
| Msa0459170 | Msa0564890 | 0.879415 | 1.269661e-69 | -8.615850e-47 |
| Msa0459170 | Msa0639610 | 0.814358 | 1.604482e-51 | -8.615850e-47 |
| Msa0459170 | Msa0678930 | 0.800688 | 1.281543e-48 | -8.615850e-47 |
| Msa0459170 | Msa0726140 | 0.865443 | 5.884246e-65 | -8.615850e-47 |
| Msa0459170 | Msa0729800 | 0.809822 | 1.565115e-50 | -8.615850e-47 |
| Msa0459170 | Msa0733290 | 0.802086 | 6.627852e-49 | -8.615850e-47 |
| Msa0459170 | Msa0743810 | 0.851414 | 9.016822e-61 | -8.615850e-47 |
| Msa0459170 | Msa0745060 | 0.834297 | 3.251637e-56 | -8.615850e-47 |
| Msa0459170 | Msa0767720 | 0.831794 | 1.363981e-55 | -8.615850e-47 |
| Msa0459170 | Msa0783160 | 0.846019 | 2.824692e-59 | -8.615850e-47 |
| Msa0459170 | Msa0787600 | 0.831638 | 1.490028e-55 | -8.615850e-47 |
| Msa0459170 | Msa0808010 | 0.823721 | 1.189333e-53 | -8.615850e-47 |
| Msa0459170 | Msa0808020 | 0.816660 | 4.931547e-52 | -8.615850e-47 |
| Msa0459170 | Msa0811080 | 0.802588 | 5.223936e-49 | -8.615850e-47 |
| Msa0459170 | Msa0825860 | 0.842264 | 2.876339e-58 | -8.615850e-47 |
| Msa0459170 | Msa0833300 | 0.802618 | 5.150649e-49 | -8.615850e-47 |
| Msa0459170 | Msa0846330 | 0.828638 | 8.045154e-55 | -8.615850e-47 |
| Msa0459170 | Msa0846350 | 0.842006 | 3.365292e-58 | -8.615850e-47 |
| Msa0459170 | Msa0848960 | 0.803945 | 2.736430e-49 | -8.615850e-47 |
| Msa0459170 | Msa0858980 | 0.802941 | 4.417327e-49 | -8.615850e-47 |
| Msa0459170 | Msa0865320 | 0.823631 | 1.248286e-53 | -8.615850e-47 |
| Msa0459170 | Msa0885290 | 0.835817 | 1.345829e-56 | -8.615850e-47 |
| Msa0459170 | Msa0888370 | 0.814786 | 1.289934e-51 | -8.615850e-47 |
| Msa0459170 | Msa0896080 | 0.802705 | 4.941787e-49 | -8.615850e-47 |
| Msa0459170 | Msa0914280 | 0.802580 | 5.244256e-49 | -8.615850e-47 |
| Msa0459170 | Msa0956270 | 0.809743 | 1.627480e-50 | -8.615850e-47 |
| Msa0459170 | Msa0981370 | 0.837660 | 4.558484e-57 | -8.615850e-47 |
| Msa0459170 | Msa0989060 | 0.820000 | 8.644154e-53 | -8.615850e-47 |
| Msa0459170 | Msa0994030 | 0.800304 | 1.534774e-48 | -8.615850e-47 |
| Msa0459170 | Msa1016280 | 0.862319 | 5.513210e-64 | -8.615850e-47 |
| Msa0459170 | Msa1025360 | 0.875191 | 3.742590e-68 | -8.615850e-47 |
| Msa0459170 | Msa1025370 | 0.869786 | 2.385501e-66 | -8.615850e-47 |
| Msa0459170 | Msa1044830 | 0.874461 | 6.631251e-68 | -8.615850e-47 |
| Msa0459170 | Msa1044840 | 0.876558 | 1.269267e-68 | -8.615850e-47 |
| Msa0459170 | Msa1060840 | 0.834105 | 3.633010e-56 | -8.615850e-47 |
| Msa0459170 | Msa1092990 | 0.808446 | 3.085190e-50 | -8.615850e-47 |
| Msa0459170 | Msa1100980 | 0.833082 | 6.541500e-56 | -8.615850e-47 |
| Msa0459170 | Msa1109440 | 0.842022 | 3.331985e-58 | -8.615850e-47 |
| Msa0459170 | Msa1121200 | 0.855649 | 5.473632e-62 | -8.615850e-47 |
| Msa0459170 | Msa1135740 | 0.821415 | 4.087871e-53 | -8.615850e-47 |
| Msa0459170 | Msa1136090 | 0.825570 | 4.361465e-54 | -8.615850e-47 |
| Msa0459170 | Msa1146110 | 0.819095 | 1.390255e-52 | -8.615850e-47 |
| Msa0459170 | Msa1149360 | 0.816613 | 5.053969e-52 | -8.615850e-47 |
| Msa0459170 | Msa1150370 | 0.836669 | 8.173871e-57 | -8.615850e-47 |
| Msa0459170 | Msa1173920 | 0.823675 | 1.219148e-53 | -8.615850e-47 |
| Msa0459170 | Msa1187940 | 0.828868 | 7.075488e-55 | -8.615850e-47 |
| Msa0459170 | Msa1187950 | 0.834213 | 3.414146e-56 | -8.615850e-47 |
| Msa0459170 | Msa1192640 | 0.862570 | 4.616535e-64 | -8.615850e-47 |
| Msa0459170 | Msa1230430 | 0.802451 | 5.574499e-49 | -8.615850e-47 |
| Msa0459170 | Msa1246880 | 0.811577 | 6.531211e-51 | -8.615850e-47 |
| Msa0459170 | Msa1269670 | 0.832436 | 9.464943e-56 | -8.615850e-47 |
| Msa0459170 | Msa1270230 | 0.852962 | 3.271247e-61 | -8.615850e-47 |
| Msa0459170 | Msa1270240 | 0.845993 | 2.871215e-59 | -8.615850e-47 |
| Msa0459170 | Msa1270270 | 0.819529 | 1.107442e-52 | -8.615850e-47 |
| Msa0459170 | Msa1273230 | 0.805486 | 1.304074e-49 | -8.615850e-47 |
| Msa0459170 | Msa1283330 | 0.863940 | 1.737987e-64 | -8.615850e-47 |
| Msa0459170 | Msa1285930 | 0.870022 | 1.997157e-66 | -8.615850e-47 |
| Msa0459170 | Msa1287800 | 0.861676 | 8.679555e-64 | -8.615850e-47 |
| Msa0459170 | Msa1287810 | 0.869755 | 2.440562e-66 | -8.615850e-47 |
| Msa0459170 | Msa1287830 | 0.811612 | 6.418108e-51 | -8.615850e-47 |
| Msa0459170 | Msa1289430 | 0.837890 | 3.978615e-57 | -8.615850e-47 |
| Msa0459170 | Msa1307890 | 0.844962 | 5.463022e-59 | -8.615850e-47 |
| Msa0459170 | Msa1320300 | 0.812632 | 3.844692e-51 | -8.615850e-47 |
| Msa0459170 | Msa1340590 | 0.811748 | 5.995358e-51 | -8.615850e-47 |
| Msa0459170 | Msa1369430 | 0.815475 | 9.070097e-52 | -8.615850e-47 |
| Msa0459170 | Msa1397580 | 0.816565 | 5.180201e-52 | -8.615850e-47 |
| Msa0459170 | Msa1404000 | 0.854157 | 1.484272e-61 | -8.615850e-47 |
| Msa0459170 | Msa1415310 | 0.856516 | 3.051887e-62 | -8.615850e-47 |
| Msa0459170 | Msa1422180 | 0.831039 | 2.092244e-55 | -8.615850e-47 |
| Msa0459170 | Msa1433120 | 0.834605 | 2.721553e-56 | -8.615850e-47 |
| Msa0267000 | Msa0459170 | 0.876651 | 1.178521e-68 | -8.615850e-47 |
| Msa0267010 | Msa0459170 | 0.878703 | 2.266391e-69 | -8.615850e-47 |
| Msa0270520 | Msa0459170 | 0.831920 | 1.269410e-55 | -8.615850e-47 |
| Msa0309020 | Msa0459170 | 0.813492 | 2.490897e-51 | -8.615850e-47 |
| Msa0313760 | Msa0459170 | 0.857693 | 1.371809e-62 | -8.615850e-47 |
| Msa0342240 | Msa0459170 | 0.847414 | 1.173782e-59 | -8.615850e-47 |
| Msa0360510 | Msa0459170 | 0.846708 | 1.832999e-59 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0459170 | MtrunA17_Chr3g0107461 | 88.820 | 161 | 18 | 0 | 1 | 161 | 1 | 161 | 1.71e-104 | 295 |
| Msa0459170 | MtrunA17_Chr4g0031421 | 78.195 | 133 | 29 | 0 | 29 | 161 | 1 | 133 | 5.28e-71 | 210 |
| Msa0459170 | MtrunA17_Chr3g0105791 | 77.444 | 133 | 30 | 0 | 29 | 161 | 1 | 133 | 2.92e-70 | 208 |
| Msa0459170 | MtrunA17_Chr3g0107381 | 55.484 | 155 | 66 | 2 | 1 | 154 | 1 | 153 | 3.30e-55 | 173 |
| Msa0459170 | MtrunA17_Chr3g0110131 | 49.367 | 158 | 79 | 1 | 1 | 158 | 1 | 157 | 5.11e-42 | 140 |
| Msa0459170 | MtrunA17_Chr2g0296861 | 47.742 | 155 | 80 | 1 | 1 | 155 | 1 | 154 | 2.36e-40 | 133 |
| Msa0459170 | MtrunA17_Chr2g0283941 | 42.500 | 160 | 92 | 0 | 1 | 160 | 1 | 160 | 2.77e-40 | 135 |
| Msa0459170 | MtrunA17_Chr4g0014321 | 38.961 | 154 | 94 | 0 | 1 | 154 | 1 | 154 | 2.10e-37 | 128 |
| Msa0459170 | MtrunA17_Chr4g0014131 | 44.720 | 161 | 89 | 0 | 1 | 161 | 1 | 161 | 1.21e-36 | 126 |
| Msa0459170 | MtrunA17_Chr8g0351751 | 41.772 | 158 | 90 | 1 | 1 | 156 | 1 | 158 | 2.05e-36 | 125 |
| Msa0459170 | MtrunA17_Chr5g0432691 | 39.610 | 154 | 92 | 1 | 1 | 154 | 1 | 153 | 3.23e-36 | 123 |
| Msa0459170 | MtrunA17_Chr4g0014031 | 44.099 | 161 | 90 | 0 | 1 | 161 | 1 | 161 | 4.56e-36 | 124 |
| Msa0459170 | MtrunA17_Chr3g0107331 | 41.722 | 151 | 87 | 1 | 3 | 153 | 2 | 151 | 8.89e-35 | 119 |
| Msa0459170 | MtrunA17_Chr3g0106421 | 46.512 | 129 | 69 | 0 | 29 | 157 | 1 | 129 | 3.49e-34 | 119 |
| Msa0459170 | MtrunA17_Chr4g0014121 | 40.881 | 159 | 94 | 0 | 1 | 159 | 29 | 187 | 1.10e-33 | 117 |
| Msa0459170 | MtrunA17_Chr2g0296831 | 49.180 | 122 | 62 | 0 | 1 | 122 | 1 | 122 | 2.79e-32 | 111 |
| Msa0459170 | MtrunA17_Chr3g0106501 | 44.697 | 132 | 73 | 0 | 29 | 160 | 1 | 132 | 2.87e-32 | 112 |
| Msa0459170 | MtrunA17_Chr4g0013041 | 37.013 | 154 | 95 | 1 | 1 | 154 | 1 | 152 | 3.20e-31 | 111 |
| Msa0459170 | MtrunA17_Chr3g0107351 | 37.419 | 155 | 96 | 1 | 3 | 157 | 2 | 155 | 1.17e-30 | 108 |
| Msa0459170 | MtrunA17_Chr5g0420351 | 38.217 | 157 | 95 | 2 | 1 | 157 | 1 | 155 | 7.91e-30 | 106 |
| Msa0459170 | MtrunA17_Chr1g0188681 | 39.506 | 162 | 90 | 4 | 1 | 159 | 1 | 157 | 6.93e-29 | 103 |
| Msa0459170 | MtrunA17_Chr3g0107341 | 38.158 | 152 | 93 | 1 | 3 | 154 | 2 | 152 | 1.08e-28 | 103 |
| Msa0459170 | MtrunA17_Chr3g0107361 | 38.158 | 152 | 93 | 1 | 3 | 154 | 2 | 152 | 1.14e-28 | 103 |
| Msa0459170 | MtrunA17_Chr1g0197461 | 34.839 | 155 | 99 | 2 | 3 | 157 | 2 | 154 | 1.94e-26 | 97.8 |
| Msa0459170 | MtrunA17_Chr1g0188661 | 37.037 | 162 | 98 | 3 | 1 | 161 | 1 | 159 | 2.87e-26 | 97.4 |
| Msa0459170 | MtrunA17_Chr4g0012761 | 37.267 | 161 | 99 | 2 | 1 | 161 | 1 | 159 | 3.34e-26 | 97.4 |
| Msa0459170 | MtrunA17_Chr7g0218151 | 35.404 | 161 | 102 | 2 | 1 | 161 | 1 | 159 | 1.62e-24 | 92.8 |
| Msa0459170 | MtrunA17_Chr1g0188651 | 36.646 | 161 | 98 | 2 | 1 | 161 | 1 | 157 | 2.72e-24 | 92.4 |
| Msa0459170 | MtrunA17_Chr1g0197261 | 32.911 | 158 | 102 | 3 | 1 | 157 | 1 | 155 | 1.42e-23 | 90.5 |
| Msa0459170 | MtrunA17_Chr1g0197321 | 32.704 | 159 | 102 | 3 | 1 | 157 | 1 | 156 | 1.53e-22 | 87.8 |
| Msa0459170 | MtrunA17_Chr1g0193311 | 36.076 | 158 | 97 | 3 | 1 | 157 | 1 | 155 | 2.56e-22 | 87.4 |
| Msa0459170 | MtrunA17_Chr4g0008701 | 28.889 | 135 | 92 | 1 | 16 | 150 | 19 | 149 | 2.20e-14 | 69.7 |
| Msa0459170 | MtrunA17_Chr7g0267601 | 28.947 | 114 | 81 | 0 | 5 | 118 | 8 | 121 | 3.29e-13 | 66.2 |
| Msa0459170 | MtrunA17_Chr6g0457851 | 29.204 | 113 | 73 | 3 | 1 | 107 | 11 | 122 | 1.10e-11 | 61.6 |
| Msa0459170 | MtrunA17_Chr1g0212151 | 28.448 | 116 | 76 | 3 | 1 | 110 | 1 | 115 | 2.52e-11 | 60.5 |
| Msa0459170 | MtrunA17_Chr3g0128721 | 26.056 | 142 | 96 | 2 | 16 | 153 | 19 | 155 | 4.04e-11 | 60.1 |
| Msa0459170 | MtrunA17_Chr2g0279821 | 30.702 | 114 | 75 | 2 | 1 | 113 | 1 | 111 | 8.83e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0459170 | AT5G48670.1 | 49.359 | 156 | 76 | 2 | 1 | 155 | 1 | 154 | 1.17e-37 | 131 |
| Msa0459170 | AT3G05860.2 | 40.789 | 152 | 85 | 2 | 1 | 150 | 1 | 149 | 4.33e-30 | 108 |
| Msa0459170 | AT1G65330.1 | 41.216 | 148 | 86 | 1 | 3 | 150 | 2 | 148 | 5.28e-30 | 110 |
| Msa0459170 | AT3G05860.3 | 52.525 | 99 | 47 | 0 | 1 | 99 | 1 | 99 | 1.61e-29 | 108 |
| Msa0459170 | AT3G05860.1 | 52.525 | 99 | 47 | 0 | 1 | 99 | 1 | 99 | 2.13e-29 | 108 |
| Msa0459170 | AT1G65300.1 | 39.735 | 151 | 90 | 1 | 5 | 155 | 4 | 153 | 3.29e-29 | 108 |
| Msa0459170 | AT5G27810.1 | 53.933 | 89 | 41 | 0 | 29 | 117 | 1 | 89 | 2.06e-28 | 102 |
| Msa0459170 | AT5G26650.1 | 35.948 | 153 | 97 | 1 | 5 | 157 | 3 | 154 | 5.09e-24 | 96.3 |
| Msa0459170 | AT5G27960.1 | 34.641 | 153 | 99 | 1 | 5 | 157 | 3 | 154 | 5.70e-23 | 92.8 |
| Msa0459170 | AT1G22590.2 | 33.125 | 160 | 90 | 2 | 1 | 157 | 1 | 146 | 1.22e-21 | 85.9 |
| Msa0459170 | AT1G31640.1 | 38.272 | 162 | 86 | 4 | 3 | 158 | 2 | 155 | 3.19e-21 | 89.4 |
| Msa0459170 | AT1G31630.1 | 35.484 | 155 | 92 | 4 | 3 | 154 | 2 | 151 | 4.86e-21 | 87.8 |
| Msa0459170 | AT5G26630.1 | 33.548 | 155 | 102 | 1 | 1 | 155 | 1 | 154 | 1.72e-20 | 84.3 |
| Msa0459170 | AT2G28700.1 | 32.667 | 150 | 100 | 1 | 1 | 150 | 1 | 149 | 3.52e-20 | 85.5 |
| Msa0459170 | AT5G06500.1 | 33.333 | 147 | 88 | 3 | 1 | 147 | 1 | 137 | 1.44e-18 | 79.7 |
| Msa0459170 | AT5G26580.1 | 37.624 | 101 | 49 | 1 | 1 | 101 | 1 | 87 | 7.83e-14 | 68.2 |
| Msa0459170 | AT2G40210.1 | 33.663 | 101 | 67 | 0 | 1 | 101 | 1 | 101 | 4.49e-13 | 65.9 |
| Msa0459170 | AT5G26865.1 | 41.667 | 72 | 41 | 1 | 24 | 94 | 2 | 73 | 1.43e-11 | 58.9 |
Find 36 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTCATTTCCTGCTTTC+TGG | 0.066665 | 3_3:+55542752 | None:intergenic |
| ATTCATACCACAGAATTTAA+TGG | 0.196701 | 3_3:-55542714 | Msa0459170:CDS |
| CAGGTTTGGCCATCAGATTC+AGG | 0.224706 | 3_3:-55542909 | Msa0459170:CDS |
| CTGAATGTTATCCTCCTCTT+CGG | 0.350265 | 3_3:+55543228 | None:intergenic |
| GTTCAGGAGTTTGCATGAAC+TGG | 0.380322 | 3_3:-55542865 | Msa0459170:CDS |
| GATGAAATTAGCACTCTTTG+TGG | 0.382102 | 3_3:-55542978 | Msa0459170:CDS |
| CTGAATCTGATGGCCAAACC+TGG | 0.394792 | 3_3:+55542910 | None:intergenic |
| TGAATGTTATCCTCCTCTTC+GGG | 0.395416 | 3_3:+55543229 | None:intergenic |
| GAAGTGTGATATCCAGGTTC+AGG | 0.406864 | 3_3:-55542881 | Msa0459170:CDS |
| AATGAAATGATGAATGATAT+TGG | 0.442815 | 3_3:+55542734 | None:intergenic |
| AGGTTTGGCCATCAGATTCA+GGG | 0.444978 | 3_3:-55542908 | Msa0459170:CDS |
| ACAATCCTCAAGCCCAGGTT+TGG | 0.451431 | 3_3:-55542923 | Msa0459170:CDS |
| TATTCTTGAGGCAAAGCATT+GGG | 0.452810 | 3_3:-55542812 | Msa0459170:CDS |
| GAGGACAAATCATTAAGATC+AGG | 0.474701 | 3_3:+55542675 | None:intergenic |
| GGGTTAGAAGTGTGATATCC+AGG | 0.506627 | 3_3:-55542887 | Msa0459170:CDS |
| CACTATCATGGCTAGAGGTA+AGG | 0.508320 | 3_3:-55543277 | None:intergenic |
| AAGGAAGGAAACCAGAAAGC+AGG | 0.520416 | 3_3:-55542763 | Msa0459170:CDS |
| CAAATTCTCAGCAATAAAAG+AGG | 0.520681 | 3_3:+55542656 | None:intergenic |
| GGAGGATAACATTCAGAAAG+AGG | 0.525464 | 3_3:-55543221 | Msa0459170:CDS |
| TTCATGCAAACTCCTGAACC+TGG | 0.526718 | 3_3:+55542869 | None:intergenic |
| ACTCTTTGTGGAATAGAAGC+TGG | 0.543874 | 3_3:-55542966 | Msa0459170:CDS |
| TAAATATCCATTAAATTCTG+TGG | 0.554522 | 3_3:+55542707 | None:intergenic |
| GTATTCTTGAGGCAAAGCAT+TGG | 0.555152 | 3_3:-55542813 | Msa0459170:CDS |
| GATGGCCAAACCTGGGCTTG+AGG | 0.558655 | 3_3:+55542918 | None:intergenic |
| GCAAAACAATCCTCAAGCCC+AGG | 0.573782 | 3_3:-55542928 | Msa0459170:CDS |
| TGAATCTGATGGCCAAACCT+GGG | 0.592740 | 3_3:+55542911 | None:intergenic |
| GAGGTCGATCAAAAGATGAA+AGG | 0.605953 | 3_3:-55542630 | Msa0459170:CDS |
| CTTCTAACCCCTGAATCTGA+TGG | 0.608216 | 3_3:+55542900 | None:intergenic |
| ATGAGCAGTTGATGAAACAA+AGG | 0.608842 | 3_3:-55542782 | Msa0459170:CDS |
| ACATAACATGTGACCCGAAG+AGG | 0.618667 | 3_3:-55543242 | Msa0459170:CDS |
| GGTTTGGCCATCAGATTCAG+GGG | 0.621539 | 3_3:-55542907 | Msa0459170:CDS |
| GCAGTTGATGAAACAAAGGA+AGG | 0.627441 | 3_3:-55542778 | Msa0459170:CDS |
| TATTGCTGAGAATTTGAGGG+AGG | 0.639350 | 3_3:-55542649 | Msa0459170:CDS |
| ATCCTCACTATCATGGCTAG+AGG | 0.659018 | 3_3:-55543282 | None:intergenic |
| TAACATGTGACCCGAAGAGG+AGG | 0.659370 | 3_3:-55543239 | Msa0459170:CDS |
| TGGATCAAGAGGTATTCTTG+AGG | 0.709854 | 3_3:-55542824 | Msa0459170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATGGATTTTTTTTATTA+GGG | - | chr3_3:55542865-55542884 | Msa0459170:CDS | 10.0% |
| !!! | TAAATTTGGAATTTTACTAT+AGG | - | chr3_3:55542832-55542851 | Msa0459170:CDS | 15.0% |
| !! | TATAGGTTATATATTGTTTA+TGG | - | chr3_3:55542849-55542868 | Msa0459170:CDS | 15.0% |
| !!! | GTTTATGGATTTTTTTTATT+AGG | - | chr3_3:55542864-55542883 | Msa0459170:CDS | 15.0% |
| !! | AATGAAATGATGAATGATAT+TGG | + | chr3_3:55543150-55543169 | None:intergenic | 20.0% |
| !! | TAAATATCCATTAAATTCTG+TGG | + | chr3_3:55543177-55543196 | None:intergenic | 20.0% |
| ! | ATTCATACCACAGAATTTAA+TGG | - | chr3_3:55543167-55543186 | Msa0459170:intron | 25.0% |
| !! | TTTTATTGCTGAGAATTTGA+GGG | - | chr3_3:55543229-55543248 | Msa0459170:CDS | 25.0% |
| ACATTCAGAAAGAGGAAAAA+TGG | - | chr3_3:55542668-55542687 | Msa0459170:CDS | 30.0% | |
| TATGTATGATATGCAAAGAG+AGG | + | chr3_3:55542704-55542723 | None:intergenic | 30.0% | |
| ACTGGAACAAAGTAAAAAGA+TGG | - | chr3_3:55543034-55543053 | Msa0459170:intron | 30.0% | |
| CAAATTCTCAGCAATAAAAG+AGG | + | chr3_3:55543228-55543247 | None:intergenic | 30.0% | |
| ! | CTTTTATTGCTGAGAATTTG+AGG | - | chr3_3:55543228-55543247 | Msa0459170:CDS | 30.0% |
| GATGAAATTAGCACTCTTTG+TGG | - | chr3_3:55542903-55542922 | Msa0459170:CDS | 35.0% | |
| GGAACAAAGTAAAAAGATGG+TGG | - | chr3_3:55543037-55543056 | Msa0459170:intron | 35.0% | |
| !! | TAAAAAGATGGTGGATCAAG+AGG | - | chr3_3:55543046-55543065 | Msa0459170:intron | 35.0% |
| ! | TATTCTTGAGGCAAAGCATT+GGG | - | chr3_3:55543069-55543088 | Msa0459170:intron | 35.0% |
| !! | ATGAGCAGTTGATGAAACAA+AGG | - | chr3_3:55543099-55543118 | Msa0459170:intron | 35.0% |
| GAGGACAAATCATTAAGATC+AGG | + | chr3_3:55543209-55543228 | None:intergenic | 35.0% | |
| TGAATGTTATCCTCCTCTTC+GGG | + | chr3_3:55542655-55542674 | None:intergenic | 40.0% | |
| CTGAATGTTATCCTCCTCTT+CGG | + | chr3_3:55542656-55542675 | None:intergenic | 40.0% | |
| GGAGGATAACATTCAGAAAG+AGG | - | chr3_3:55542660-55542679 | Msa0459170:CDS | 40.0% | |
| ACTCTTTGTGGAATAGAAGC+TGG | - | chr3_3:55542915-55542934 | Msa0459170:CDS | 40.0% | |
| !! | TGGATCAAGAGGTATTCTTG+AGG | - | chr3_3:55543057-55543076 | Msa0459170:intron | 40.0% |
| GTATTCTTGAGGCAAAGCAT+TGG | - | chr3_3:55543068-55543087 | Msa0459170:intron | 40.0% | |
| !! | GCAGTTGATGAAACAAAGGA+AGG | - | chr3_3:55543103-55543122 | Msa0459170:intron | 40.0% |
| ATTGGTCATTTCCTGCTTTC+TGG | + | chr3_3:55543132-55543151 | None:intergenic | 40.0% | |
| TATTGCTGAGAATTTGAGGG+AGG | - | chr3_3:55543232-55543251 | Msa0459170:CDS | 40.0% | |
| GAGGTCGATCAAAAGATGAA+AGG | - | chr3_3:55543251-55543270 | Msa0459170:CDS | 40.0% | |
| ACATAACATGTGACCCGAAG+AGG | - | chr3_3:55542639-55542658 | Msa0459170:CDS | 45.0% | |
| TGAATCTGATGGCCAAACCT+GGG | + | chr3_3:55542973-55542992 | None:intergenic | 45.0% | |
| AGGTTTGGCCATCAGATTCA+GGG | - | chr3_3:55542973-55542992 | Msa0459170:CDS | 45.0% | |
| CTTCTAACCCCTGAATCTGA+TGG | + | chr3_3:55542984-55543003 | None:intergenic | 45.0% | |
| ! | GGGTTAGAAGTGTGATATCC+AGG | - | chr3_3:55542994-55543013 | Msa0459170:CDS | 45.0% |
| GAAGTGTGATATCCAGGTTC+AGG | - | chr3_3:55543000-55543019 | Msa0459170:intron | 45.0% | |
| TTCATGCAAACTCCTGAACC+TGG | + | chr3_3:55543015-55543034 | None:intergenic | 45.0% | |
| GTTCAGGAGTTTGCATGAAC+TGG | - | chr3_3:55543016-55543035 | Msa0459170:intron | 45.0% | |
| AAGGAAGGAAACCAGAAAGC+AGG | - | chr3_3:55543118-55543137 | Msa0459170:intron | 45.0% | |
| !!! | TAACTTTTATTTAATAAATT+TGG | - | chr3_3:55542818-55542837 | Msa0459170:CDS | 5.0% |
| TAACATGTGACCCGAAGAGG+AGG | - | chr3_3:55542642-55542661 | Msa0459170:CDS | 50.0% | |
| GCAAAACAATCCTCAAGCCC+AGG | - | chr3_3:55542953-55542972 | Msa0459170:CDS | 50.0% | |
| ACAATCCTCAAGCCCAGGTT+TGG | - | chr3_3:55542958-55542977 | Msa0459170:CDS | 50.0% | |
| CTGAATCTGATGGCCAAACC+TGG | + | chr3_3:55542974-55542993 | None:intergenic | 50.0% | |
| CAGGTTTGGCCATCAGATTC+AGG | - | chr3_3:55542972-55542991 | Msa0459170:CDS | 50.0% | |
| GGTTTGGCCATCAGATTCAG+GGG | - | chr3_3:55542974-55542993 | Msa0459170:CDS | 50.0% | |
| GATGGCCAAACCTGGGCTTG+AGG | + | chr3_3:55542966-55542985 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 55542611 | 55543292 | 55542611 | ID=Msa0459170;Name=Msa0459170 |
| chr3_3 | mRNA | 55542611 | 55543292 | 55542611 | ID=Msa0459170-mRNA-1;Parent=Msa0459170;Name=Msa0459170-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|161 |
| chr3_3 | exon | 55543214 | 55543292 | 55543214 | ID=Msa0459170-mRNA-1:exon:11006;Parent=Msa0459170-mRNA-1 |
| chr3_3 | exon | 55542611 | 55543017 | 55542611 | ID=Msa0459170-mRNA-1:exon:11005;Parent=Msa0459170-mRNA-1 |
| chr3_3 | CDS | 55543214 | 55543292 | 55543214 | ID=Msa0459170-mRNA-1:cds;Parent=Msa0459170-mRNA-1 |
| chr3_3 | CDS | 55542611 | 55543017 | 55542611 | ID=Msa0459170-mRNA-1:cds;Parent=Msa0459170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
