AlfalfaGEDB | Msa0227480

M. sativa cultivar zhongmu-4 / Msa0227480

KEGG: K11294
GO: -
PFAM: PF00583.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227480	AES70281.2	70.000	350	80	4	39	387	2	327	3.95e-148	434

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227480	sp\|Q7XTT4\|NUCL2_ORYSJ	50.000	78	38	1	311	387	431	508	7.97e-19	92.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227480	G7J1Z7	70.000	350	80	4	39	387	2	327	1.89e-148	434

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TFs/TRs:

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Gene ID	Type	Classification
Msa0227480	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012700	Msa0227480	0.804780	1.833256e-49	-8.615850e-47
Msa0013750	Msa0227480	0.829485	5.014089e-55	-8.615850e-47
Msa0014330	Msa0227480	0.820440	6.853200e-53	-8.615850e-47
Msa0015750	Msa0227480	0.861100	1.300850e-63	-8.615850e-47
Msa0016570	Msa0227480	0.852064	5.899714e-61	-8.615850e-47
Msa0016580	Msa0227480	0.838875	2.217237e-57	-8.615850e-47
Msa0056530	Msa0227480	0.811052	8.489339e-51	-8.615850e-47
Msa0071500	Msa0227480	0.813655	2.292620e-51	-8.615850e-47
Msa0092630	Msa0227480	0.833342	5.634165e-56	-8.615850e-47
Msa0102070	Msa0227480	0.892286	1.825207e-74	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227480	MtrunA17_Chr3g0098471	76.596	188	21	2	196	383	20	184	5.57e-77	236
Msa0227480	MtrunA17_Chr3g0098461	67.925	159	50	1	39	197	2	159	2.67e-71	220
Msa0227480	MtrunA17_Chr0c05g0492161	55.914	93	41	0	40	132	3	95	4.48e-28	107
Msa0227480	MtrunA17_Chr4g0038111	67.949	78	25	0	310	387	321	398	8.95e-28	115
Msa0227480	MtrunA17_Chr4g0038111	44.966	149	32	3	198	299	4	149	3.85e-11	64.7
Msa0227480	MtrunA17_Chr1g0163591	51.899	79	37	1	307	384	271	349	6.95e-19	88.6
Msa0227480	MtrunA17_Chr1g0163591	32.727	220	101	7	197	387	20	221	1.45e-18	87.8
Msa0227480	MtrunA17_Chr3g0090311	32.704	159	94	7	43	191	2	157	6.52e-14	69.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227480	AT3G18610.3	45.783	83	44	1	305	387	332	413	9.69e-18	85.5
Msa0227480	AT3G18610.1	45.783	83	44	1	305	387	362	443	1.05e-17	85.5
Msa0227480	AT3G18610.2	45.783	83	44	1	305	387	302	383	1.05e-17	85.5
Msa0227480	AT1G48920.1	42.391	92	51	2	295	385	265	355	3.37e-16	80.5
Msa0227480	AT5G13780.1	33.758	157	91	7	43	189	2	155	2.67e-12	65.5

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Find 83 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGCTGAAAAGGACTTA+AGG	0.455617	2_1:-78737765	Msa0227480:CDS
AAACGCTGAAAAGGACTTAA+GGG	0.449883	2_1:-78737764	Msa0227480:CDS
AAACTTTCAAGATCCACGTC+AGG	0.572396	2_1:+78737955	None:intergenic
AAAGAAGCAAAAGGTAGATG+AGG	0.604240	2_1:-78736122	Msa0227480:CDS
AAAGATAGGGTTAACGAAGA+TGG	0.472513	2_1:+78737819	None:intergenic
AAAGATGGGGTATGAGAAGG+AGG	0.631960	2_1:-78737459	Msa0227480:CDS
AACCCTATCTTTCGGAGAGA+AGG	0.502877	2_1:-78737808	Msa0227480:CDS
AAGAAATGTGTCCCCGGATG+TGG	0.566003	2_1:-78738034	Msa0227480:CDS
AATCCATCAGAGTTGGTCTT+CGG	0.518936	2_1:+78737494	None:intergenic
ACTCCTTCTCTCCGAAAGAT+AGG	0.337370	2_1:+78737805	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTACTCTTCTTCGAAAAGA+TGG	-	chr2_1:78735372-78735391	Msa0227480:intron	30.0%
TTACTCTTCTTCGAAAAGAT+GGG	-	chr2_1:78735373-78735392	Msa0227480:intron	30.0%
GTGAAGAGCAATTATATTGA+TGG	-	chr2_1:78735412-78735431	Msa0227480:intron	30.0%
ATCAAACTAGCCAATAAAGT+TGG	+	chr2_1:78735595-78735614	None:intergenic	30.0%
AATCCAAGTAAAACGTGAAA+AGG	-	chr2_1:78735925-78735944	Msa0227480:CDS	30.0%
TAACCACATGACAATTCAAT+AGG	-	chr2_1:78735976-78735995	Msa0227480:intron	30.0%
AAATCAGACAAGCGTTTATT+TGG	-	chr2_1:78736107-78736126	Msa0227480:CDS	30.0%
CGAAACTGTAAACTAGAAAA+CGG	-	chr2_1:78736279-78736298	Msa0227480:intron	30.0%
GAAACTGTAAACTAGAAAAC+GGG	-	chr2_1:78736280-78736299	Msa0227480:intron	30.0%
CAACAATAAAATTGGAGAAG+TGG	+	chr2_1:78736579-78736598	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	78734705	78738163	78734705	ID=Msa0227480;Name=Msa0227480
chr2_1	mRNA	78734705	78738163	78734705	ID=Msa0227480-mRNA-1;Parent=Msa0227480;Name=Msa0227480-mRNA-1;_AED=0.17;_eAED=0.18;_QI=0\|0\|0\|1\|0.85\|0.87\|8\|0\|388
chr2_1	exon	78737759	78738163	78737759	ID=Msa0227480-mRNA-1:exon:23957;Parent=Msa0227480-mRNA-1
chr2_1	exon	78737363	78737546	78737363	ID=Msa0227480-mRNA-1:exon:23956;Parent=Msa0227480-mRNA-1
chr2_1	exon	78736048	78736184	78736048	ID=Msa0227480-mRNA-1:exon:23955;Parent=Msa0227480-mRNA-1
chr2_1	exon	78735834	78735947	78735834	ID=Msa0227480-mRNA-1:exon:23954;Parent=Msa0227480-mRNA-1
chr2_1	exon	78735614	78735715	78735614	ID=Msa0227480-mRNA-1:exon:23953;Parent=Msa0227480-mRNA-1
chr2_1	exon	78735270	78735358	78735270	ID=Msa0227480-mRNA-1:exon:23952;Parent=Msa0227480-mRNA-1
chr2_1	exon	78735077	78735194	78735077	ID=Msa0227480-mRNA-1:exon:23951;Parent=Msa0227480-mRNA-1
chr2_1	exon	78734705	78734722	78734705	ID=Msa0227480-mRNA-1:exon:23950;Parent=Msa0227480-mRNA-1
chr2_1	CDS	78737759	78738163	78737759	ID=Msa0227480-mRNA-1:cds;Parent=Msa0227480-mRNA-1
chr2_1	CDS	78737363	78737546	78737363	ID=Msa0227480-mRNA-1:cds;Parent=Msa0227480-mRNA-1
chr2_1	CDS	78736048	78736184	78736048	ID=Msa0227480-mRNA-1:cds;Parent=Msa0227480-mRNA-1
chr2_1	CDS	78735834	78735947	78735834	ID=Msa0227480-mRNA-1:cds;Parent=Msa0227480-mRNA-1
chr2_1	CDS	78735614	78735715	78735614	ID=Msa0227480-mRNA-1:cds;Parent=Msa0227480-mRNA-1
chr2_1	CDS	78735270	78735358	78735270	ID=Msa0227480-mRNA-1:cds;Parent=Msa0227480-mRNA-1
chr2_1	CDS	78735077	78735194	78735077	ID=Msa0227480-mRNA-1:cds;Parent=Msa0227480-mRNA-1
chr2_1	CDS	78734705	78734722	78734705	ID=Msa0227480-mRNA-1:cds;Parent=Msa0227480-mRNA-1

Gene Sequence

>Msa0227480

ATGCTACCTTTCTACTATAATGTTTTTGGTTTTTTTGTGGATTGTTGTGCTCTTGTTGTTTCTCCTGTGAAGTCTGGAGCGGAGGAGTCACCCAAAATTCGTTATGCAAGAAATGTGTCCCCGGATGTGGTCTTAAGAAACGCAACATCTGACGACATATTTAAGATTCTCGTGGATTATCTCTTACCTGACGTGGATCTTGAAAGTTTGGAGAATGGTAAGATTCTTCAAGTCATCGCGGATGATCGCCAAGACGCTATCCTCGGTTACGTCATCTTTGATGTCGTTGAACCGAAGAGTAATGGTTGTGGTCATATCACTTCCATCTTCGTTAACCCTATCTTTCGGAGAGAAGGAGTCGCTTCTAAACTTGTGGAAAACGCTGAAAAGGACTTAAGGGATAAGCAAGGTGCTGCTTACGTCTCAGTGCCCGTTCCGAAGACCAACTCTGATGGATTACTCTTCTTCGAAAAGATGGGGTATGAGAAGGAGGCAGTGAAGAGCAATTATATTGATGGGAAGGATGATGCTAATGTCATCATGACGAAGAAGTTCGAGGGGAAGCAGATGCATTATGCGGAGACCCAAGGCAAGAGGCAAGCTGTGGAAGAAGTCGAGGAAGTTTCTTCAAAGAAGCAAAAGGTAGATGAGGTTGCTGCCAAACAAAAGGTATTGAAGGTAGTCAAAATTAGTTCGCAGGAATCCTTTCATGAATCTGAGGATGTGAACCCTGCAACAAAAGTTGTTCCATCCAAAAATGGGAGTGCACCTGTAAAGAAAGACGATCTTTATTATTCATCTTCTGAAGAATCTTCTGGTGAATCTTCTGATGAAGATGAGAAACCTGCAGCAGAAGTTGTTCCTTCCAAAATAGGAAGTGCACCCGCAAAGAAGGCTGATAGTTCTGACCAAGAATCTGACGAATCTTCTGGTGAAGATGAGCCTAATACTCCAGCTACACAAAATGTGAACAGTCGTGTGAAGACATTATATGTTGGGAACTTATCCTTCGGTGTGCAAAAATCTGACATAGAAAAATTCTTCCAAGATTGTGGAAAAGTTATTGATGTACGTCTTGCTTCTCATAAGGATGGAAAGCTTAAAGGCTTTGGACATGTTGAGTTTGCAACTGCAGAGGCAGCCCAATACGCGTGCAGGATTCTATAA

Protein sequence

>Msa0227480

MLPFYYNVFGFFVDCCALVVSPVKSGAEESPKIRYARNVSPDVVLRNATSDDIFKILVDYLLPDVDLESLENGKILQVIADDRQDAILGYVIFDVVEPKSNGCGHITSIFVNPIFRREGVASKLVENAEKDLRDKQGAAYVSVPVPKTNSDGLLFFEKMGYEKEAVKSNYIDGKDDANVIMTKKFEGKQMHYAETQGKRQAVEEVEEVSSKKQKVDEVAAKQKVLKVVKISSQESFHESEDVNPATKVVPSKNGSAPVKKDDLYYSSSEESSGESSDEDEKPAAEVVPSKIGSAPAKKADSSDQESDESSGEDEPNTPATQNVNSRVKTLYVGNLSFGVQKSDIEKFFQDCGKVIDVRLASHKDGKLKGFGHVEFATAEAAQYACRIL*