AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0365500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0365500 MtrunA17_Chr3g0107461 79.195 149 29 1 1 147 1 149 2.46e-81 236
Msa0365500 MtrunA17_Chr3g0105791 94.215 121 5 1 29 147 1 121 1.89e-79 231
Msa0365500 MtrunA17_Chr4g0031421 90.083 121 10 1 29 147 1 121 4.12e-74 218
Msa0365500 MtrunA17_Chr3g0107381 55.034 149 64 2 1 147 1 148 3.16e-51 162
Msa0365500 MtrunA17_Chr3g0110131 52.349 149 68 2 1 147 1 148 5.61e-40 134
Msa0365500 MtrunA17_Chr2g0283941 46.309 149 78 1 1 147 1 149 2.67e-39 132
Msa0365500 MtrunA17_Chr5g0432691 42.763 152 78 3 1 147 1 148 2.50e-38 127
Msa0365500 MtrunA17_Chr8g0351751 44.371 151 80 2 1 147 1 151 3.42e-36 124
Msa0365500 MtrunA17_Chr4g0014321 40.940 149 86 1 1 147 1 149 1.05e-35 123
Msa0365500 MtrunA17_Chr2g0296861 46.980 149 76 2 1 147 1 148 7.38e-35 119
Msa0365500 MtrunA17_Chr4g0014031 44.966 149 80 1 1 147 1 149 2.10e-33 117
Msa0365500 MtrunA17_Chr5g0420351 43.624 149 80 3 1 147 1 147 3.68e-33 114
Msa0365500 MtrunA17_Chr1g0188681 43.919 148 81 2 1 147 1 147 1.75e-32 112
Msa0365500 MtrunA17_Chr3g0106501 47.934 121 61 1 29 147 1 121 1.89e-32 112
Msa0365500 MtrunA17_Chr4g0013041 38.926 149 87 2 1 147 1 147 4.50e-30 108
Msa0365500 MtrunA17_Chr4g0014131 43.624 149 82 1 1 147 1 149 4.81e-30 108
Msa0365500 MtrunA17_Chr2g0296831 47.863 117 59 1 1 115 1 117 1.38e-29 104
Msa0365500 MtrunA17_Chr3g0107331 40.136 147 85 2 3 147 2 147 2.09e-29 105
Msa0365500 MtrunA17_Chr4g0014121 42.282 149 84 1 1 147 29 177 3.16e-29 105
Msa0365500 MtrunA17_Chr1g0197461 39.041 146 87 2 3 147 2 146 9.63e-28 100
Msa0365500 MtrunA17_Chr1g0188661 41.892 148 84 2 1 147 1 147 5.70e-27 98.6
Msa0365500 MtrunA17_Chr1g0193311 41.892 148 84 2 1 147 1 147 5.93e-27 98.6
Msa0365500 MtrunA17_Chr4g0012761 41.216 148 85 2 1 147 1 147 7.80e-27 98.6
Msa0365500 MtrunA17_Chr1g0188651 41.892 148 82 2 1 147 1 145 8.99e-27 98.2
Msa0365500 MtrunA17_Chr7g0218151 41.216 148 85 2 1 147 1 147 2.09e-26 97.4
Msa0365500 MtrunA17_Chr3g0107361 38.776 147 87 2 3 147 2 147 4.25e-26 96.7
Msa0365500 MtrunA17_Chr3g0106421 42.975 121 67 1 29 147 1 121 6.41e-26 97.4
Msa0365500 MtrunA17_Chr3g0107351 37.415 147 89 2 3 147 2 147 2.43e-24 92.0
Msa0365500 MtrunA17_Chr3g0107341 37.415 147 89 2 3 147 2 147 2.75e-23 89.4
Msa0365500 MtrunA17_Chr1g0197321 34.899 149 94 2 1 147 1 148 1.09e-22 87.8
Msa0365500 MtrunA17_Chr1g0197261 34.194 155 86 5 1 147 1 147 2.23e-22 87.0
Msa0365500 MtrunA17_Chr4g0008701 30.573 157 91 5 1 146 1 150 1.56e-14 69.3
Msa0365500 MtrunA17_Chr7g0267601 30.345 145 81 5 1 137 1 133 4.70e-14 68.2
Msa0365500 MtrunA17_Chr6g0457851 27.632 152 95 4 1 145 11 154 7.71e-13 64.3
Msa0365500 MtrunA17_Chr1g0212151 30.252 119 70 3 1 109 1 116 1.07e-11 61.2
Msa0365500 MtrunA17_Chr3g0128721 31.313 99 66 1 5 101 8 106 1.21e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0365500 AT5G48670.1 50.993 151 67 4 1 147 1 148 1.25e-35 125
Msa0365500 AT3G05860.2 42.384 151 80 3 1 147 1 148 7.30e-30 107
Msa0365500 AT3G05860.3 42.384 151 80 3 1 147 1 148 1.65e-29 108
Msa0365500 AT3G05860.1 42.384 151 80 3 1 147 1 148 2.45e-29 107
Msa0365500 AT1G65330.1 41.379 145 82 2 3 145 2 145 8.79e-29 107
Msa0365500 AT5G27810.1 51.685 89 41 1 29 115 1 89 2.77e-26 96.3
Msa0365500 AT1G65300.1 40.000 145 84 2 3 145 2 145 5.57e-26 99.4
Msa0365500 AT5G26650.1 45.872 109 57 1 5 111 3 111 6.08e-25 98.2
Msa0365500 AT1G31630.1 37.162 148 87 4 3 147 2 146 2.01e-24 96.7
Msa0365500 AT5G27960.1 44.954 109 58 1 5 111 3 111 3.96e-24 95.5
Msa0365500 AT1G31640.1 39.216 153 77 5 3 147 2 146 8.89e-24 95.9
Msa0365500 AT1G22590.2 39.823 113 66 1 1 111 1 113 2.45e-23 89.7
Msa0365500 AT5G26630.1 37.584 149 90 2 1 147 1 148 2.82e-21 85.9
Msa0365500 AT2G28700.1 38.835 103 61 1 1 101 1 103 4.39e-17 76.6
Msa0365500 AT5G06500.1 35.135 111 66 2 1 109 1 107 9.01e-17 74.7
Msa0365500 AT5G26580.1 39.000 100 49 1 1 100 1 88 4.26e-14 68.2
Msa0365500 AT2G40210.1 35.644 101 63 1 1 99 1 101 7.53e-13 64.7

Find 29 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCATCTTTC+TGG 0.077675 3_1:+59620806 None:intergenic
ATTCAAACCATAGAATTTAA+TGG 0.173791 3_1:-59620768 Msa0365500:CDS
CGAATTTCCATTAAATTCTA+TGG 0.218755 3_1:+59620761 None:intergenic
GAGGTTTGGCCATCAGATTC+AGG 0.258145 3_1:-59620963 Msa0365500:CDS
CGCTACCATGACTAGAAATA+AGG 0.322931 3_1:-59621362 None:intergenic
TCTTCTTGAGGCAAAACATT+AGG 0.355934 3_1:-59620866 Msa0365500:CDS
AATATAATGATCAATGATAT+TGG 0.368342 3_1:+59620788 None:intergenic
GGGTCAGAAGTGTGATATCT+AGG 0.436600 3_1:-59620941 Msa0365500:intron
TCTAGGTTTAGGAGTTTGCC+TGG 0.445921 3_1:-59620924 Msa0365500:intron
ACAACCCTCGAGTAGAGGTT+TGG 0.487846 3_1:-59620977 Msa0365500:CDS
ACGAGCAGTTGAAGAAGCAA+AGG 0.502207 3_1:-59620836 Msa0365500:CDS
AGGTTTGGCCATCAGATTCA+GGG 0.511068 3_1:-59620962 Msa0365500:CDS
AAGGGAGGAAACCAGAAAGA+TGG 0.513695 3_1:-59620817 Msa0365500:CDS
GGAGGATAACATACAGAAAG+AGG 0.518632 3_1:-59621306 Msa0365500:CDS
TTTCACCTTATTTCTAGTCA+TGG 0.525434 3_1:+59621357 None:intergenic
CGAGCAGTTGAAGAAGCAAA+GGG 0.550309 3_1:-59620835 Msa0365500:CDS
ACATGCTTCGACTCCACATA+GGG 0.581479 3_1:+59621019 None:intergenic
TATTGATGAGAATTTGAAGG+AGG 0.592173 3_1:-59620703 None:intergenic
CACATGCTTCGACTCCACAT+AGG 0.603214 3_1:+59621018 None:intergenic
AATGAAATTAGCACCCTATG+TGG 0.604835 3_1:-59621032 Msa0365500:CDS
GCAAAACAACCCTCGAGTAG+AGG 0.609086 3_1:-59620982 Msa0365500:CDS
ACATAACATGTGACTCGAAG+AGG 0.616371 3_1:-59621327 Msa0365500:CDS
CTTCTGACCCCTGAATCTGA+TGG 0.624024 3_1:+59620954 None:intergenic
GATGGCCAAACCTCTACTCG+AGG 0.634480 3_1:+59620972 None:intergenic
GGTTTGGCCATCAGATTCAG+GGG 0.657992 3_1:-59620961 Msa0365500:CDS
ATGGCCAAACCTCTACTCGA+GGG 0.659257 3_1:+59620973 None:intergenic
TAACATGTGACTCGAAGAGG+AGG 0.659370 3_1:-59621324 Msa0365500:CDS
GCAGTTGAAGAAGCAAAGGG+AGG 0.693727 3_1:-59620832 Msa0365500:CDS
TGGATCAGGAGGTCTTCTTG+AGG 0.700337 3_1:-59620878 Msa0365500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATATAGAATTTTTTTAAT+AGG - chr3_1:59620988-59621007 Msa0365500:CDS 10.0%
!! AATATAATGATCAATGATAT+TGG + chr3_1:59621274-59621293 None:intergenic 15.0%
!! AATAAACAATATACAATCGA+TGG + chr3_1:59620969-59620988 None:intergenic 20.0%
!! ATTCAAACCATAGAATTTAA+TGG - chr3_1:59621291-59621310 Msa0365500:intron 20.0%
!!! TTTTATTGATGAGAATTTGA+AGG - chr3_1:59621353-59621372 Msa0365500:CDS 20.0%
!! TACATGCTTTGTTGAAATTA+TGG + chr3_1:59620802-59620821 None:intergenic 25.0%
!!! TGTGATATTTCTAGGAAAAA+AGG + chr3_1:59620854-59620873 None:intergenic 25.0%
! TGTTAACATGTGATATTTCT+AGG + chr3_1:59620862-59620881 None:intergenic 25.0%
! ACATGAACAAAGTAAAAAGA+TGG - chr3_1:59621158-59621177 Msa0365500:intron 25.0%
! CGAATTTCCATTAAATTCTA+TGG + chr3_1:59621301-59621320 None:intergenic 25.0%
ACATACAGAAAGAGGAAAAA+TGG - chr3_1:59620761-59620780 Msa0365500:CDS 30.0%
! TTTTTACTTTGTTCATGTCC+AGG + chr3_1:59621156-59621175 None:intergenic 30.0%
TGAACAAAGTAAAAAGATGG+TGG - chr3_1:59621161-59621180 Msa0365500:intron 30.0%
! GCTTTGTTGAAATTATGGAG+AGG + chr3_1:59620797-59620816 None:intergenic 35.0%
AATGAAATTAGCACCCTATG+TGG - chr3_1:59621027-59621046 Msa0365500:CDS 35.0%
GAAGTGTGATATCTAGGTTT+AGG - chr3_1:59621124-59621143 Msa0365500:intron 35.0%
!! AAGTAAAAAGATGGTGGATC+AGG - chr3_1:59621167-59621186 Msa0365500:intron 35.0%
TCTTCTTGAGGCAAAACATT+AGG - chr3_1:59621193-59621212 Msa0365500:intron 35.0%
ATTGGTCATTTCCATCTTTC+TGG + chr3_1:59621256-59621275 None:intergenic 35.0%
ACATAACATGTGACTCGAAG+AGG - chr3_1:59620732-59620751 Msa0365500:CDS 40.0%
GGAGGATAACATACAGAAAG+AGG - chr3_1:59620753-59620772 Msa0365500:CDS 40.0%
!! TAAAAAGATGGTGGATCAGG+AGG - chr3_1:59621170-59621189 Msa0365500:intron 40.0%
TAACATGTGACTCGAAGAGG+AGG - chr3_1:59620735-59620754 Msa0365500:CDS 45.0%
ACATGCTTCGACTCCACATA+GGG + chr3_1:59621043-59621062 None:intergenic 45.0%
AGGTTTGGCCATCAGATTCA+GGG - chr3_1:59621097-59621116 Msa0365500:intron 45.0%
! GGGTCAGAAGTGTGATATCT+AGG - chr3_1:59621118-59621137 Msa0365500:intron 45.0%
TCTAGGTTTAGGAGTTTGCC+TGG - chr3_1:59621135-59621154 Msa0365500:intron 45.0%
ACGAGCAGTTGAAGAAGCAA+AGG - chr3_1:59621223-59621242 Msa0365500:intron 45.0%
CGAGCAGTTGAAGAAGCAAA+GGG - chr3_1:59621224-59621243 Msa0365500:intron 45.0%
AAGGGAGGAAACCAGAAAGA+TGG - chr3_1:59621242-59621261 Msa0365500:intron 45.0%
CACATGCTTCGACTCCACAT+AGG + chr3_1:59621044-59621063 None:intergenic 50.0%
GCAAAACAACCCTCGAGTAG+AGG - chr3_1:59621077-59621096 Msa0365500:intron 50.0%
ACAACCCTCGAGTAGAGGTT+TGG - chr3_1:59621082-59621101 Msa0365500:intron 50.0%
ATGGCCAAACCTCTACTCGA+GGG + chr3_1:59621089-59621108 None:intergenic 50.0%
GAGGTTTGGCCATCAGATTC+AGG - chr3_1:59621096-59621115 Msa0365500:intron 50.0%
GGTTTGGCCATCAGATTCAG+GGG - chr3_1:59621098-59621117 Msa0365500:intron 50.0%
CTTCTGACCCCTGAATCTGA+TGG + chr3_1:59621108-59621127 None:intergenic 50.0%
TGGATCAGGAGGTCTTCTTG+AGG - chr3_1:59621181-59621200 Msa0365500:intron 50.0%
GCAGTTGAAGAAGCAAAGGG+AGG - chr3_1:59621227-59621246 Msa0365500:intron 50.0%
GATGGCCAAACCTCTACTCG+AGG + chr3_1:59621090-59621109 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 59620704 59621377 59620704 ID=Msa0365500;Name=Msa0365500
chr3_1 mRNA 59620704 59621377 59620704 ID=Msa0365500-mRNA-1;Parent=Msa0365500;Name=Msa0365500-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|0|0|3|0|147
chr3_1 exon 59621299 59621377 59621299 ID=Msa0365500-mRNA-1:exon:11406;Parent=Msa0365500-mRNA-1
chr3_1 exon 59620942 59621071 59620942 ID=Msa0365500-mRNA-1:exon:11405;Parent=Msa0365500-mRNA-1
chr3_1 exon 59620704 59620935 59620704 ID=Msa0365500-mRNA-1:exon:11404;Parent=Msa0365500-mRNA-1
chr3_1 CDS 59621299 59621377 59621299 ID=Msa0365500-mRNA-1:cds;Parent=Msa0365500-mRNA-1
chr3_1 CDS 59620942 59621071 59620942 ID=Msa0365500-mRNA-1:cds;Parent=Msa0365500-mRNA-1
chr3_1 CDS 59620704 59620935 59620704 ID=Msa0365500-mRNA-1:cds;Parent=Msa0365500-mRNA-1
Gene Sequence

>Msa0365500

ATGACTAGAAATAAGGTGAAACTAGCATACATAACATGTGACTCGAAGAGGAGGATAACATACAGAAAGAGGAAAAATGGAATTATGAAGAAGATTAATGAAATTAGCACCCTATGTGGAGTCGAAGCATGTGCAATAATTTCAGAGCAAAACAACCCTCGAGTAGAGGTTTGGCCATCAGATTCAGGGGTCAGAAGTGTGATATCTAGGAGTTTGCCTGGACATGAACAAAGTAAAAAGATGGTGGATCAGGAGGTCTTCTTGAGGCAAAACATTAGGAAAGTTTACGAGCAGTTGAAGAAGCAAAGGGAGGAAACCAGAAAGATGGAAATGACCAATATCATTGATCATTATATTCAAACCATAGAATTTAATGGAAATTCGATGAGCAAATCTGATCTTAATGATTTTTCATCTTTTATTGATGAGAATTTGAAGGAG

Protein sequence

>Msa0365500

MTRNKVKLAYITCDSKRRITYRKRKNGIMKKINEISTLCGVEACAIISEQNNPRVEVWPSDSGVRSVISRSLPGHEQSKKMVDQEVFLRQNIRKVYEQLKKQREETRKMEMTNIIDHYIQTIEFNGNSMSKSDLNDFSSFIDENLKE