AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0626190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0626190 MtrunA17_Chr4g0001971 95.694 209 9 0 1 209 1 209 6.90e-145 402
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0626190 AT1G80940.1 62.037 216 58 5 2 209 14 213 8.67e-78 232
Msa0626190 AT1G80940.3 83.465 127 20 1 65 190 16 142 1.15e-68 207
Msa0626190 AT1G80940.2 60.000 145 44 4 2 138 14 152 3.91e-47 153
Msa0626190 AT1G80940.4 51.220 123 46 4 2 116 14 130 3.83e-29 106

Find 63 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCTTCATCTCCCACTTCT+TGG 0.189164 4_3:+5542985 None:intergenic
TAACCTTTCTGTTCATTTCT+TGG 0.189299 4_3:+5543484 None:intergenic
CGGCCGTTGCTGTTCTTTAT+AGG 0.207088 4_3:+5543639 None:intergenic
ATTTCATTTCTTGCATGTTT+TGG 0.252992 4_3:-5545591 Msa0626190:five_prime_UTR
TGCCGAAGTTTCTGAGCTTT+CGG 0.303182 4_3:-5543694 Msa0626190:CDS
TATCAGCATCTTTGTTTGTA+AGG 0.306286 4_3:-5545707 None:intergenic
CTGTTCTTTATAGGTTCTAA+TGG 0.315483 4_3:+5543648 None:intergenic
AGGAGTATCTTCCGGGTTGC+CGG 0.334216 4_3:+5543740 None:intergenic
GAGTCCTGCATTTCCACATT+AGG 0.337528 4_3:+5543717 None:intergenic
TAAATTATAATTCATCTTCA+AGG 0.358878 4_3:+5542954 None:intergenic
CATTAGGAGGAGTATCTTCC+GGG 0.367822 4_3:+5543733 None:intergenic
AAAATCTGAGGAGGTGAAAC+AGG 0.371893 4_3:+5545507 None:intergenic
TTCATCTTCAAGGTTCATCT+TGG 0.411081 4_3:+5542964 None:intergenic
TATGGTCATGGATGATGCTT+TGG 0.425253 4_3:-5545541 Msa0626190:CDS
TATCCAAAGGGATGGCTGAT+TGG 0.428780 4_3:-5543564 Msa0626190:CDS
TGTAAGGTTGTTGAAAACTT+TGG 0.430176 4_3:-5545691 Msa0626190:five_prime_UTR
ATATAGTAGTAGTATCAATA+TGG 0.432236 4_3:-5542852 Msa0626190:three_prime_UTR
TTTCCAATCAGCCATCCCTT+TGG 0.434651 4_3:+5543561 None:intergenic
GGAGTATCTTCCGGGTTGCC+GGG 0.439882 4_3:+5543741 None:intergenic
TTGCAGGATAGAGAAATAGA+TGG 0.443528 4_3:-5543179 Msa0626190:intron
GTTACAAGAAACTGAGCAAA+TGG 0.465931 4_3:-5543018 Msa0626190:CDS
TATAGTAGTAGTATCAATAT+GGG 0.468650 4_3:-5542851 Msa0626190:three_prime_UTR
TCCTCCTAATGTGGAAATGC+AGG 0.470382 4_3:-5543721 Msa0626190:CDS
GGCCAAGAAGTGGGAGATGA+AGG 0.479804 4_3:-5542987 Msa0626190:CDS
TAGTTTGCAGACTCAAAATC+AGG 0.486553 4_3:+5543585 None:intergenic
AGCTAGCGGGAGGAATTTAG+AGG 0.489631 4_3:-5543793 Msa0626190:CDS
ACATTAGGAGGAGTATCTTC+CGG 0.492696 4_3:+5543732 None:intergenic
AGATGGACTAAACGAACAGC+TGG 0.507521 4_3:-5543162 Msa0626190:CDS
TGAGCTTTCGGAGCGTCGAA+AGG 0.531699 4_3:-5543682 Msa0626190:CDS
TCCTGCATTTCCACATTAGG+AGG 0.532301 4_3:+5543720 None:intergenic
TGGAAATCTAGAAAGAGATC+AGG 0.533990 4_3:-5545483 Msa0626190:intron
GAGTCTGCAAACTATCCAAA+GGG 0.537534 4_3:-5543576 Msa0626190:CDS
CTAGATTTCCAGAAAATCTG+AGG 0.541060 4_3:+5545495 None:intergenic
ACTGAGCAAATGGCAGATGA+AGG 0.541063 4_3:-5543008 Msa0626190:CDS
TAAAACACCACCCGGCAACC+CGG 0.548227 4_3:-5543751 Msa0626190:CDS
GTCATGGATGATGCTTTGGA+TGG 0.553546 4_3:-5545537 Msa0626190:CDS
AACAGCTGGAGGAGGATTCG+CGG 0.559765 4_3:-5543148 Msa0626190:CDS
GGAAGATACTCCTCCTAATG+TGG 0.567411 4_3:-5543730 Msa0626190:CDS
TGAGTCTGCAAACTATCCAA+AGG 0.573463 4_3:-5543577 Msa0626190:CDS
CTCCGAAAGCTCAGAAACTT+CGG 0.590975 4_3:+5543692 None:intergenic
TGTCCAAGAAATGAACAGAA+AGG 0.592222 4_3:-5543487 Msa0626190:intron
ATAGTAGTAGTATCAATATG+GGG 0.595387 4_3:-5542850 Msa0626190:three_prime_UTR
CTGCAAACTATCCAAAGGGA+TGG 0.596246 4_3:-5543572 Msa0626190:CDS
AAGCACAAGCATGCACACAC+TGG 0.598231 4_3:+5542928 None:intergenic
ACGTGTTCACCACAGCTAGC+GGG 0.599138 4_3:-5543806 Msa0626190:intron
GGCAGATGAAGGCCAAGAAG+TGG 0.599174 4_3:-5542997 Msa0626190:CDS
GCAGACTCAAAATCAGGTGG+AGG 0.602642 4_3:+5543591 None:intergenic
AAGACTCGGCAGAGAGTCGT+CGG 0.607206 4_3:+5543619 None:intergenic
AGGTGGAGGAAGCAAAGACT+CGG 0.620390 4_3:+5543605 None:intergenic
ACTAAACGAACAGCTGGAGG+AGG 0.624134 4_3:-5543156 Msa0626190:CDS
GCAGATGAAGGCCAAGAAGT+GGG 0.625863 4_3:-5542996 Msa0626190:CDS
AGGTTCTAATGGTTCAAACA+AGG 0.637581 4_3:+5543659 None:intergenic
GCACTTGCAAGTGCAGCTTG+TGG 0.640595 4_3:-5543117 Msa0626190:CDS
ATGTGGTCGAAAGCATGCGA+AGG 0.652930 4_3:-5543515 Msa0626190:CDS
GATACAGCTAAAACACCACC+CGG 0.658820 4_3:-5543759 Msa0626190:CDS
TGGACTAAACGAACAGCTGG+AGG 0.659762 4_3:-5543159 Msa0626190:CDS
GTATCTTCCGGGTTGCCGGG+TGG 0.662272 4_3:+5543744 None:intergenic
TTTGCAGACTCAAAATCAGG+TGG 0.663167 4_3:+5543588 None:intergenic
GAACCTATAAAGAACAGCAA+CGG 0.668853 4_3:-5543642 Msa0626190:CDS
TAGTAGTAGTATCAATATGG+GGG 0.676157 4_3:-5542849 Msa0626190:three_prime_UTR
GATTTCCAGAAAATCTGAGG+AGG 0.685426 4_3:+5545498 None:intergenic
AAAGCTTGTCAATGTTGATG+TGG 0.687893 4_3:-5543532 Msa0626190:CDS
TGTTCACCACAGCTAGCGGG+AGG 0.700309 4_3:-5543803 Msa0626190:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAAATAACAATTTTATTA+AGG + chr4_3:5543814-5543833 None:intergenic 10.0%
!!! CTTTATTATTTTATCTTGTA+TGG - chr4_3:5543111-5543130 Msa0626190:CDS 15.0%
!!! ATACGTTAGTATAATATTTT+TGG - chr4_3:5543445-5543464 Msa0626190:intron 15.0%
!! TTACATGAAAATAATAGTAT+TGG + chr4_3:5543598-5543617 None:intergenic 15.0%
!!! AATATAAAGTATTGTTTTGT+TGG + chr4_3:5543767-5543786 None:intergenic 15.0%
!! AAAATTGAAACATTCATATA+TGG + chr4_3:5544065-5544084 None:intergenic 15.0%
!! GATGTAAAATTGAATTATAA+TGG + chr4_3:5544121-5544140 None:intergenic 15.0%
!! TAAATTATAATTCATCTTCA+AGG + chr4_3:5545483-5545502 None:intergenic 15.0%
!! TAAAAGATGAAGAAGAAAAA+AGG + chr4_3:5542979-5542998 None:intergenic 20.0%
!! GTATGGTATTTATAGTATTT+AGG - chr4_3:5543128-5543147 Msa0626190:CDS 20.0%
!!! GGTGATTTTTTAATTGAATT+TGG - chr4_3:5543171-5543190 Msa0626190:CDS 20.0%
!!! ATTTGTATGATTTGAAATTG+AGG - chr4_3:5543246-5543265 Msa0626190:intron 20.0%
!! TATTATACTAACGTATTGTT+TGG + chr4_3:5543441-5543460 None:intergenic 20.0%
!!! AATTGTTATTTTTGTGATCA+TGG - chr4_3:5543820-5543839 Msa0626190:intron 20.0%
!!! ATTTTACATCTTCTTTTTCA+AGG - chr4_3:5544131-5544150 Msa0626190:intron 20.0%
!!! TTTTTCAAGGAAACTTAAAT+GGG - chr4_3:5544144-5544163 Msa0626190:intron 20.0%
!! TTTACTATCTCTAAATAAGA+AGG - chr4_3:5544321-5544340 Msa0626190:intron 20.0%
!! TGGTTACACTTAAATTATTA+CGG - chr4_3:5544432-5544451 Msa0626190:intron 20.0%
!! TAAATGAATTGCAAAAAGAA+TGG - chr4_3:5544603-5544622 Msa0626190:intron 20.0%
!!! TGAATTTAAGGTTGTTAAAA+AGG + chr4_3:5544988-5545007 None:intergenic 20.0%
!! ATATAGTAGTAGTATCAATA+TGG - chr4_3:5545582-5545601 Msa0626190:five_prime_UTR 20.0%
!! TATAGTAGTAGTATCAATAT+GGG - chr4_3:5545583-5545602 Msa0626190:five_prime_UTR 20.0%
!! ATTTCATTTCTTGCATGTTT+TGG - chr4_3:5542843-5542862 Msa0626190:three_prime_UTR 25.0%
!!! AGAAAATTTTGAAGCAACTA+TGG - chr4_3:5542875-5542894 Msa0626190:three_prime_UTR 25.0%
!!! GTGTTTAATTGAGCTTTTTT+TGG - chr4_3:5543150-5543169 Msa0626190:CDS 25.0%
!!! TAACATTTTGTTTTGTAAGC+TGG - chr4_3:5543286-5543305 Msa0626190:intron 25.0%
!!! GCTTTATTTGTTATTATGGT+TGG - chr4_3:5543411-5543430 Msa0626190:intron 25.0%
!! ATTGAACATGTTTTCAATCT+AGG + chr4_3:5543554-5543573 None:intergenic 25.0%
! ATTGAATTTCTTGATGTACA+AGG - chr4_3:5543856-5543875 Msa0626190:intron 25.0%
! TTGAATTTCTTGATGTACAA+GGG - chr4_3:5543857-5543876 Msa0626190:intron 25.0%
! ATATGGTAGATGAACAATAA+AGG + chr4_3:5544048-5544067 None:intergenic 25.0%
!!! CTTTTTCAAGGAAACTTAAA+TGG - chr4_3:5544143-5544162 Msa0626190:intron 25.0%
!!! ATTTTCCTTCTTATTTTCTG+CGG - chr4_3:5544271-5544290 Msa0626190:intron 25.0%
!!! CATTCTTAGCTTTGAATTTA+AGG + chr4_3:5545000-5545019 None:intergenic 25.0%
! CAAGTGGATAAAAAATAAAC+TGG + chr4_3:5545114-5545133 None:intergenic 25.0%
! ATAGTAGTAGTATCAATATG+GGG - chr4_3:5545584-5545603 Msa0626190:five_prime_UTR 25.0%
TGTAAGGTTGTTGAAAACTT+TGG - chr4_3:5542743-5542762 Msa0626190:three_prime_UTR 30.0%
TAAAACCAAAAAGACTTGCA+AGG - chr4_3:5542791-5542810 Msa0626190:three_prime_UTR 30.0%
! ATTTTCCTTGCAAGTCTTTT+TGG + chr4_3:5542799-5542818 None:intergenic 30.0%
!! CGCTGCTTTATTTGTTATTA+TGG - chr4_3:5543407-5543426 Msa0626190:intron 30.0%
! ATAGTATTGGACTATTGTGT+TGG + chr4_3:5543585-5543604 None:intergenic 30.0%
CTAATGCATTAATGCTGTTA+TGG + chr4_3:5543647-5543666 None:intergenic 30.0%
AATTTAAGCACGATTAACTC+CGG + chr4_3:5543684-5543703 None:intergenic 30.0%
! TGTTGGTTACTTTCATAAAG+AGG + chr4_3:5543750-5543769 None:intergenic 30.0%
TGAATTTCTTGATGTACAAG+GGG - chr4_3:5543858-5543877 Msa0626190:intron 30.0%
! TCTTGTTATGTGTTGAGTTA+TGG - chr4_3:5543922-5543941 Msa0626190:intron 30.0%
! CTTGTTATGTGTTGAGTTAT+GGG - chr4_3:5543923-5543942 Msa0626190:intron 30.0%
ATTAAGAAGTCACATATGCA+AGG + chr4_3:5544010-5544029 None:intergenic 30.0%
AAATAAGAAGGAAGAAAACC+TGG - chr4_3:5544333-5544352 Msa0626190:intron 30.0%
! CTGTTCTTTATAGGTTCTAA+TGG + chr4_3:5544789-5544808 None:intergenic 30.0%
TAACCTTTCTGTTCATTTCT+TGG + chr4_3:5544953-5544972 None:intergenic 30.0%
AGGTTGTTAAAAAGGAAACT+TGG + chr4_3:5544980-5544999 None:intergenic 30.0%
GTGGATAAAAAATAAACTGG+TGG + chr4_3:5545111-5545130 None:intergenic 30.0%
! TCGTATATTGGTTGAAATTC+AGG + chr4_3:5545151-5545170 None:intergenic 30.0%
!!! CAACACTTTTTCTCGTATAT+TGG + chr4_3:5545163-5545182 None:intergenic 30.0%
TAGTAGTAGTATCAATATGG+GGG - chr4_3:5545585-5545604 Msa0626190:five_prime_UTR 30.0%
!! CTTACTTTTCTCAAAAACAG+AGG - chr4_3:5545625-5545644 Msa0626190:five_prime_UTR 30.0%
!!! TTTTGAAGCAACTATGGTCA+TGG - chr4_3:5542881-5542900 Msa0626190:three_prime_UTR 35.0%
CTAGATTTCCAGAAAATCTG+AGG + chr4_3:5542942-5542961 None:intergenic 35.0%
TGGAAATCTAGAAAGAGATC+AGG - chr4_3:5542951-5542970 Msa0626190:three_prime_UTR 35.0%
GTACAATAGTGGTTAACAGT+TGG - chr4_3:5543368-5543387 Msa0626190:intron 35.0%
ATTAGATCTCATCAGAACTC+CGG - chr4_3:5543662-5543681 Msa0626190:CDS 35.0%
CAACACAAAAACTTTCTAGG+AGG + chr4_3:5543708-5543727 None:intergenic 35.0%
GTGCAACACAAAAACTTTCT+AGG + chr4_3:5543711-5543730 None:intergenic 35.0%
GAATTTCTTGATGTACAAGG+GGG - chr4_3:5543859-5543878 Msa0626190:intron 35.0%
AATGGGAGATGAATCACTAA+AGG - chr4_3:5544161-5544180 Msa0626190:intron 35.0%
TATCACCGCAGAAAATAAGA+AGG + chr4_3:5544279-5544298 None:intergenic 35.0%
GCACAAACAATTCAGATTGA+AGG - chr4_3:5544372-5544391 Msa0626190:intron 35.0%
! AGGTTCTAATGGTTCAAACA+AGG + chr4_3:5544778-5544797 None:intergenic 35.0%
GAACCTATAAAGAACAGCAA+CGG - chr4_3:5544792-5544811 Msa0626190:intron 35.0%
! TAGTTTGCAGACTCAAAATC+AGG + chr4_3:5544852-5544871 None:intergenic 35.0%
AAAGCTTGTCAATGTTGATG+TGG - chr4_3:5544902-5544921 Msa0626190:intron 35.0%
TGTCCAAGAAATGAACAGAA+AGG - chr4_3:5544947-5544966 Msa0626190:intron 35.0%
!! CGAGAAAAAGTGTTGAAAAG+AGG - chr4_3:5545168-5545187 Msa0626190:intron 35.0%
TTGCAGGATAGAGAAATAGA+TGG - chr4_3:5545255-5545274 Msa0626190:intron 35.0%
GTTACAAGAAACTGAGCAAA+TGG - chr4_3:5545416-5545435 Msa0626190:intron 35.0%
TTCATCTTCAAGGTTCATCT+TGG + chr4_3:5545473-5545492 None:intergenic 35.0%
TATGGTCATGGATGATGCTT+TGG - chr4_3:5542893-5542912 Msa0626190:three_prime_UTR 40.0%
AAAATCTGAGGAGGTGAAAC+AGG + chr4_3:5542930-5542949 None:intergenic 40.0%
! TTTCACCTCCTCAGATTTTC+TGG - chr4_3:5542931-5542950 Msa0626190:three_prime_UTR 40.0%
GATTTCCAGAAAATCTGAGG+AGG + chr4_3:5542939-5542958 None:intergenic 40.0%
! AAGAGGTGCAACACATAAAG+CGG + chr4_3:5543733-5543752 None:intergenic 40.0%
TAAAAACCCATGTGGCTGTT+TGG - chr4_3:5543884-5543903 Msa0626190:intron 40.0%
CATATGCAAGGAAACCAACA+TGG + chr4_3:5543998-5544017 None:intergenic 40.0%
!! GTGTGTTTGCTATCCAACAT+CGG - chr4_3:5544394-5544413 Msa0626190:intron 40.0%
ATGCTTCATAGATAGATCCC+TGG - chr4_3:5544491-5544510 Msa0626190:intron 40.0%
!!! CTCTTTTTTGAACCCTACCA+TGG + chr4_3:5544517-5544536 None:intergenic 40.0%
!! ACATTAGGAGGAGTATCTTC+CGG + chr4_3:5544705-5544724 None:intergenic 40.0%
TTTGCAGACTCAAAATCAGG+TGG + chr4_3:5544849-5544868 None:intergenic 40.0%
TGAGTCTGCAAACTATCCAA+AGG - chr4_3:5544857-5544876 Msa0626190:intron 40.0%
GAGTCTGCAAACTATCCAAA+GGG - chr4_3:5544858-5544877 Msa0626190:intron 40.0%
AGACTGTTAGAGTCTGCAAT+TGG + chr4_3:5545047-5545066 None:intergenic 40.0%
GGAGTCTTACTAAAGTCAAG+TGG + chr4_3:5545130-5545149 None:intergenic 40.0%
! TATTGCTAGCATTTTCCGCA+TGG - chr4_3:5545202-5545221 Msa0626190:intron 40.0%
! CATTTGACGACTTTTGTTGC+AGG - chr4_3:5545239-5545258 Msa0626190:intron 40.0%
! GTCATGGATGATGCTTTGGA+TGG - chr4_3:5542897-5542916 Msa0626190:three_prime_UTR 45.0%
TACGCTTCTCTCTCTTCTCT+TGG + chr4_3:5543351-5543370 None:intergenic 45.0%
! GAGAGAGAAGCGTACAATAG+TGG - chr4_3:5543357-5543376 Msa0626190:intron 45.0%
AGGGGGAATAAAAACCCATG+TGG - chr4_3:5543876-5543895 Msa0626190:intron 45.0%
CACTATCCAAACAGCCACAT+GGG + chr4_3:5543893-5543912 None:intergenic 45.0%
ACACTATCCAAACAGCCACA+TGG + chr4_3:5543894-5543913 None:intergenic 45.0%
!! TAAAGAGGGTTGGTTGAAGG+TGG + chr4_3:5543966-5543985 None:intergenic 45.0%
!! CACTAAAGAGGGTTGGTTGA+AGG + chr4_3:5543969-5543988 None:intergenic 45.0%
CATGGAGGCTACACTAAAGA+GGG + chr4_3:5543980-5543999 None:intergenic 45.0%
ACATGGAGGCTACACTAAAG+AGG + chr4_3:5543981-5544000 None:intergenic 45.0%
CTTTAGTGTAGCCTCCATGT+TGG - chr4_3:5543981-5544000 Msa0626190:intron 45.0%
ATGCAAGGAAACCAACATGG+AGG + chr4_3:5543995-5544014 None:intergenic 45.0%
GCTACATAGAGCTCTATACC+AGG + chr4_3:5544354-5544373 None:intergenic 45.0%
ACAAACACGACACTGACACA+TGG + chr4_3:5544461-5544480 None:intergenic 45.0%
GATACAGCTAAAACACCACC+CGG - chr4_3:5544675-5544694 Msa0626190:intron 45.0%
!! CATTAGGAGGAGTATCTTCC+GGG + chr4_3:5544704-5544723 None:intergenic 45.0%
GGAAGATACTCCTCCTAATG+TGG - chr4_3:5544704-5544723 Msa0626190:intron 45.0%
TCCTCCTAATGTGGAAATGC+AGG - chr4_3:5544713-5544732 Msa0626190:intron 45.0%
TCCTGCATTTCCACATTAGG+AGG + chr4_3:5544717-5544736 None:intergenic 45.0%
GAGTCCTGCATTTCCACATT+AGG + chr4_3:5544720-5544739 None:intergenic 45.0%
TGCCGAAGTTTCTGAGCTTT+CGG - chr4_3:5544740-5544759 Msa0626190:intron 45.0%
CTCCGAAAGCTCAGAAACTT+CGG + chr4_3:5544745-5544764 None:intergenic 45.0%
CTGCAAACTATCCAAAGGGA+TGG - chr4_3:5544862-5544881 Msa0626190:intron 45.0%
TATCCAAAGGGATGGCTGAT+TGG - chr4_3:5544870-5544889 Msa0626190:intron 45.0%
TTTCCAATCAGCCATCCCTT+TGG + chr4_3:5544876-5544895 None:intergenic 45.0%
! GTCGTCAAATGCATTAGCCT+CGG + chr4_3:5545230-5545249 None:intergenic 45.0%
!! AGATGGACTAAACGAACAGC+TGG - chr4_3:5545272-5545291 Msa0626190:intron 45.0%
ACTGAGCAAATGGCAGATGA+AGG - chr4_3:5545426-5545445 Msa0626190:intron 45.0%
!!! TTTTTTTATGATAAAAATTT+TGG + chr4_3:5543472-5543491 None:intergenic 5.0%
AGTTATGGGACAGCTAACCC+TGG - chr4_3:5543937-5543956 Msa0626190:intron 50.0%
GAGGCTACACTAAAGAGGGT+TGG + chr4_3:5543976-5543995 None:intergenic 50.0%
ATCGGCACGACACGATACAT+TGG - chr4_3:5544412-5544431 Msa0626190:intron 50.0%
CATAGATAGATCCCTGGCCA+TGG - chr4_3:5544497-5544516 Msa0626190:intron 50.0%
ATAGATCCCTGGCCATGGTA+GGG - chr4_3:5544502-5544521 Msa0626190:intron 50.0%
TTTGAACCCTACCATGGCCA+GGG + chr4_3:5544511-5544530 None:intergenic 50.0%
! TTTTGAACCCTACCATGGCC+AGG + chr4_3:5544512-5544531 None:intergenic 50.0%
AACGTGTTCACCACAGCTAG+CGG - chr4_3:5544627-5544646 Msa0626190:intron 50.0%
TAAATTCCTCCCGCTAGCTG+TGG + chr4_3:5544640-5544659 None:intergenic 50.0%
AGCTAGCGGGAGGAATTTAG+AGG - chr4_3:5544641-5544660 Msa0626190:intron 50.0%
! CGGCCGTTGCTGTTCTTTAT+AGG + chr4_3:5544798-5544817 None:intergenic 50.0%
AGGTGGAGGAAGCAAAGACT+CGG + chr4_3:5544832-5544851 None:intergenic 50.0%
GCAGACTCAAAATCAGGTGG+AGG + chr4_3:5544846-5544865 None:intergenic 50.0%
! ATGTGGTCGAAAGCATGCGA+AGG - chr4_3:5544919-5544938 Msa0626190:intron 50.0%
TGGACTAAACGAACAGCTGG+AGG - chr4_3:5545275-5545294 Msa0626190:intron 50.0%
ACTAAACGAACAGCTGGAGG+AGG - chr4_3:5545278-5545297 Msa0626190:intron 50.0%
GCAGATGAAGGCCAAGAAGT+GGG - chr4_3:5545438-5545457 Msa0626190:intron 50.0%
AAGCACAAGCATGCACACAC+TGG + chr4_3:5545509-5545528 None:intergenic 50.0%
TTGGTTGAAGGTGGAGGCCA+GGG + chr4_3:5543957-5543976 None:intergenic 55.0%
!! AGAGGGTTGGTTGAAGGTGG+AGG + chr4_3:5543963-5543982 None:intergenic 55.0%
! GTATCGTGTCGTGCCGATGT+TGG + chr4_3:5544410-5544429 None:intergenic 55.0%
GATAGATCCCTGGCCATGGT+AGG - chr4_3:5544501-5544520 Msa0626190:intron 55.0%
ACGTGTTCACCACAGCTAGC+GGG - chr4_3:5544628-5544647 Msa0626190:intron 55.0%
TAAAACACCACCCGGCAACC+CGG - chr4_3:5544683-5544702 Msa0626190:intron 55.0%
!! AGGAGTATCTTCCGGGTTGC+CGG + chr4_3:5544697-5544716 None:intergenic 55.0%
!! TGAGCTTTCGGAGCGTCGAA+AGG - chr4_3:5544752-5544771 Msa0626190:intron 55.0%
AAGACTCGGCAGAGAGTCGT+CGG + chr4_3:5544818-5544837 None:intergenic 55.0%
! GCATTTTCCGCATGGAACCG+AGG - chr4_3:5545210-5545229 Msa0626190:intron 55.0%
! GCATTAGCCTCGGTTCCATG+CGG + chr4_3:5545220-5545239 None:intergenic 55.0%
AACAGCTGGAGGAGGATTCG+CGG - chr4_3:5545286-5545305 Msa0626190:intron 55.0%
GCACTTGCAAGTGCAGCTTG+TGG - chr4_3:5545317-5545336 Msa0626190:intron 55.0%
GGCAGATGAAGGCCAAGAAG+TGG - chr4_3:5545437-5545456 Msa0626190:intron 55.0%
GGCCAAGAAGTGGGAGATGA+AGG - chr4_3:5545447-5545466 Msa0626190:intron 55.0%
GGCCTTCATCTCCCACTTCT+TGG + chr4_3:5545452-5545471 None:intergenic 55.0%
GTTGGTTGAAGGTGGAGGCC+AGG + chr4_3:5543958-5543977 None:intergenic 60.0%
TGTTCACCACAGCTAGCGGG+AGG - chr4_3:5544631-5544650 Msa0626190:intron 60.0%
! GGAGTATCTTCCGGGTTGCC+GGG + chr4_3:5544696-5544715 None:intergenic 60.0%
GTATCTTCCGGGTTGCCGGG+TGG + chr4_3:5544693-5544712 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 5542736 5545720 5542736 ID=Msa0626190;Name=Msa0626190
chr4_3 mRNA 5542736 5545720 5542736 ID=Msa0626190-mRNA-1;Parent=Msa0626190;Name=Msa0626190-mRNA-1;_AED=0.37;_eAED=0.37;_QI=158|1|1|1|1|1|3|235|209
chr4_3 exon 5542736 5543195 5542736 ID=Msa0626190-mRNA-1:exon:1550;Parent=Msa0626190-mRNA-1
chr4_3 exon 5543488 5543813 5543488 ID=Msa0626190-mRNA-1:exon:1549;Parent=Msa0626190-mRNA-1
chr4_3 exon 5545484 5545720 5545484 ID=Msa0626190-mRNA-1:exon:1548;Parent=Msa0626190-mRNA-1
chr4_3 five_prime_UTR 5545563 5545720 5545563 ID=Msa0626190-mRNA-1:five_prime_utr;Parent=Msa0626190-mRNA-1
chr4_3 CDS 5545484 5545562 5545484 ID=Msa0626190-mRNA-1:cds;Parent=Msa0626190-mRNA-1
chr4_3 CDS 5543488 5543813 5543488 ID=Msa0626190-mRNA-1:cds;Parent=Msa0626190-mRNA-1
chr4_3 CDS 5542971 5543195 5542971 ID=Msa0626190-mRNA-1:cds;Parent=Msa0626190-mRNA-1
chr4_3 three_prime_UTR 5542736 5542970 5542736 ID=Msa0626190-mRNA-1:three_prime_utr;Parent=Msa0626190-mRNA-1
Gene Sequence

>Msa0626190

ATGGTCATGGATGATGCTTTGGATGGTTTCTCTCCTGTTTCACCTCCTCAGATTTTCTGGAAATCTAGAAAGAGATCAGCTAGCGGGAGGAATTTAGAGGTATCATCAGAAGATACAGCTAAAACACCACCCGGCAACCCGGAAGATACTCCTCCTAATGTGGAAATGCAGGACTCTGCCGAAGTTTCTGAGCTTTCGGAGCGTCGAAAGGCCTTGTTTGAACCATTAGAACCTATAAAGAACAGCAACGGCCGACGACTCTCTGCCGAGTCTTTGCTTCCTCCACCTGATTTTGAGTCTGCAAACTATCCAAAGGGATGGCTGATTGGAAAGAAACGAAAGCTTGTCAATGTTGATGTGGTCGAAAGCATGCGAAGGATTGCTGTCCAAGAAATGAACAGAAAGGATAGAGAAATAGATGGACTAAACGAACAGCTGGAGGAGGATTCGCGGTGTCTAGAGCACTTGCAAGTGCAGCTTGTGGACGAAAGAAGCAAACGCTCTCGACTCGAGAGAGAAAATGAAATGCTTCAAGATCAAGTAAACATGCTTATGAACATGTTACAAGAAACTGAGCAAATGGCAGATGAAGGCCAAGAAGTGGGAGATGAAGGCCAAGATGAACCTTGA
Protein sequence

>Msa0626190

MVMDDALDGFSPVSPPQIFWKSRKRSASGRNLEVSSEDTAKTPPGNPEDTPPNVEMQDSAEVSELSERRKALFEPLEPIKNSNGRRLSAESLLPPPDFESANYPKGWLIGKKRKLVNVDVVESMRRIAVQEMNRKDREIDGLNEQLEEDSRCLEHLQVQLVDERSKRSRLERENEMLQDQVNMLMNMLQETEQMADEGQEVGDEGQDEP*