AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1378470


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1378470 MtrunA17_Chr4g0019521 77.027 74 16 1 9 81 43 116 4.96e-35 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 8 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCACAACAAAAGGGTGAT+TGG 0.404912 tig0020061:+54948 Msa1378470:CDS
AATAAGCACATACTAACTTC+TGG 0.421785 tig0020061:-55021 None:intergenic
GAATTTAAGTCCACAACAAA+AGG 0.428735 tig0020061:+54940 Msa1378470:CDS
ATAAGCACATACTAACTTCT+GGG 0.459372 tig0020061:-55020 None:intergenic
AAATTCTTCATCTCTTGTTG+TGG 0.464351 tig0020061:-54923 None:intergenic
TTGAGAAGCTAATTGGGAAG+AGG 0.524139 tig0020061:+54900 Msa1378470:CDS
AATTTAAGTCCACAACAAAA+GGG 0.599106 tig0020061:+54941 Msa1378470:CDS
TACTGAGAAAATCTTTAGAG+TGG 0.610701 tig0020061:+55721 Msa1378470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATAAATAATTATGTTTTT+GGG - tig0020061:55416-55435 None:intergenic 10.0%
!!! AGAATAAATAATTATGTTTT+TGG - tig0020061:55417-55436 None:intergenic 10.0%
!! GAAAAAAATAAAATATGTTA+TGG + tig0020061:55491-55510 Msa1378470:intron 10.0%
!!! TTATTTTTAGATTAATGTTT+AGG + tig0020061:55588-55607 Msa1378470:intron 10.0%
!!! GAATAAATAATTATGTTTTT+GGG - tig0020061:55416-55435 None:intergenic 10.0%
!!! AGAATAAATAATTATGTTTT+TGG - tig0020061:55417-55436 None:intergenic 10.0%
!! GAAAAAAATAAAATATGTTA+TGG + tig0020061:55491-55510 Msa1378470:intron 10.0%
!!! TTATTTTTAGATTAATGTTT+AGG + tig0020061:55588-55607 Msa1378470:intron 10.0%
!! AAATATACATTTAACATCAA+GGG - tig0020061:54825-54844 None:intergenic 15.0%
!! AAAATATACATTTAACATCA+AGG - tig0020061:54826-54845 None:intergenic 15.0%
!!! TATATTTTCCTAACTTAAAA+AGG - tig0020061:55158-55177 None:intergenic 15.0%
!! AAATATACATTTAACATCAA+GGG - tig0020061:54825-54844 None:intergenic 15.0%
!! AAAATATACATTTAACATCA+AGG - tig0020061:54826-54845 None:intergenic 15.0%
!!! TATATTTTCCTAACTTAAAA+AGG - tig0020061:55158-55177 None:intergenic 15.0%
!! AATATACATTTAACATCAAG+GGG - tig0020061:54824-54843 None:intergenic 20.0%
!!! AAATGTATATTTTGTAGTAG+TGG + tig0020061:54833-54852 Msa1378470:intron 20.0%
!! GAAAATAAATTTGTAAACAC+TGG + tig0020061:55132-55151 Msa1378470:intron 20.0%
!! AATCTAAAAATAACAGAGAA+TGG - tig0020061:55581-55600 None:intergenic 20.0%
!!! TTTGAAATCAAATTCATGAA+CGG + tig0020061:55640-55659 Msa1378470:intron 20.0%
!! AATATACATTTAACATCAAG+GGG - tig0020061:54824-54843 None:intergenic 20.0%
!!! AAATGTATATTTTGTAGTAG+TGG + tig0020061:54833-54852 Msa1378470:intron 20.0%
!! GAAAATAAATTTGTAAACAC+TGG + tig0020061:55132-55151 Msa1378470:intron 20.0%
!! AATCTAAAAATAACAGAGAA+TGG - tig0020061:55581-55600 None:intergenic 20.0%
!!! TTTGAAATCAAATTCATGAA+CGG + tig0020061:55640-55659 Msa1378470:intron 20.0%
!!! TTTTAATTTTCACATCAGTG+CGG - tig0020061:54800-54819 None:intergenic 25.0%
!!! AATGATTTTGAGAAGCTAAT+TGG + tig0020061:54893-54912 Msa1378470:CDS 25.0%
!!! ATGATTTTGAGAAGCTAATT+GGG + tig0020061:54894-54913 Msa1378470:CDS 25.0%
! AATTTAAGTCCACAACAAAA+GGG + tig0020061:54941-54960 Msa1378470:CDS 25.0%
! GATCTATAAAAGAATTATCG+TGG + tig0020061:55180-55199 Msa1378470:intron 25.0%
! AGGATACAGAGAAATATAAA+GGG - tig0020061:55441-55460 None:intergenic 25.0%
! TAGGATACAGAGAAATATAA+AGG - tig0020061:55442-55461 None:intergenic 25.0%
!! TCTAAAGATTTTCTCAGTAT+TGG - tig0020061:55720-55739 None:intergenic 25.0%
!!! TTTTAATTTTCACATCAGTG+CGG - tig0020061:54800-54819 None:intergenic 25.0%
!!! AATGATTTTGAGAAGCTAAT+TGG + tig0020061:54893-54912 Msa1378470:CDS 25.0%
!!! ATGATTTTGAGAAGCTAATT+GGG + tig0020061:54894-54913 Msa1378470:CDS 25.0%
! AATTTAAGTCCACAACAAAA+GGG + tig0020061:54941-54960 Msa1378470:CDS 25.0%
! GATCTATAAAAGAATTATCG+TGG + tig0020061:55180-55199 Msa1378470:intron 25.0%
! AGGATACAGAGAAATATAAA+GGG - tig0020061:55441-55460 None:intergenic 25.0%
! TAGGATACAGAGAAATATAA+AGG - tig0020061:55442-55461 None:intergenic 25.0%
!! TCTAAAGATTTTCTCAGTAT+TGG - tig0020061:55720-55739 None:intergenic 25.0%
AAATTCTTCATCTCTTGTTG+TGG - tig0020061:54926-54945 None:intergenic 30.0%
GAATTTAAGTCCACAACAAA+AGG + tig0020061:54940-54959 Msa1378470:CDS 30.0%
ATAAGCACATACTAACTTCT+GGG - tig0020061:55023-55042 None:intergenic 30.0%
AATAAGCACATACTAACTTC+TGG - tig0020061:55024-55043 None:intergenic 30.0%
AACTACCATACATGTACATT+AGG - tig0020061:55064-55083 None:intergenic 30.0%
!! ATGATAAGCTTGTTGGAATA+CGG + tig0020061:55330-55349 Msa1378470:intron 30.0%
AACTCTCATATCCATATTCA+TGG + tig0020061:55386-55405 Msa1378470:intron 30.0%
! AAATTCATGAACGGTTTTCA+AGG + tig0020061:55649-55668 Msa1378470:intron 30.0%
!! GATTTTCTCAGTATTGGATT+GGG - tig0020061:55714-55733 None:intergenic 30.0%
!! AGATTTTCTCAGTATTGGAT+TGG - tig0020061:55715-55734 None:intergenic 30.0%
! TACTGAGAAAATCTTTAGAG+TGG + tig0020061:55721-55740 Msa1378470:CDS 30.0%
AAATTCTTCATCTCTTGTTG+TGG - tig0020061:54926-54945 None:intergenic 30.0%
GAATTTAAGTCCACAACAAA+AGG + tig0020061:54940-54959 Msa1378470:CDS 30.0%
ATAAGCACATACTAACTTCT+GGG - tig0020061:55023-55042 None:intergenic 30.0%
AATAAGCACATACTAACTTC+TGG - tig0020061:55024-55043 None:intergenic 30.0%
AACTACCATACATGTACATT+AGG - tig0020061:55064-55083 None:intergenic 30.0%
!! ATGATAAGCTTGTTGGAATA+CGG + tig0020061:55330-55349 Msa1378470:intron 30.0%
AACTCTCATATCCATATTCA+TGG + tig0020061:55386-55405 Msa1378470:intron 30.0%
! AAATTCATGAACGGTTTTCA+AGG + tig0020061:55649-55668 Msa1378470:intron 30.0%
!! GATTTTCTCAGTATTGGATT+GGG - tig0020061:55714-55733 None:intergenic 30.0%
!! AGATTTTCTCAGTATTGGAT+TGG - tig0020061:55715-55734 None:intergenic 30.0%
! TACTGAGAAAATCTTTAGAG+TGG + tig0020061:55721-55740 Msa1378470:CDS 30.0%
TAGTATGTGCTTATTGTGCT+TGG + tig0020061:55029-55048 Msa1378470:intron 35.0%
CTATGCCTAATGTACATGTA+TGG + tig0020061:55056-55075 Msa1378470:intron 35.0%
!!! AACACTGGCCTTTTTAAGTT+AGG + tig0020061:55147-55166 Msa1378470:intron 35.0%
! GCGTTGATGAAATATATGAG+TGG + tig0020061:55242-55261 Msa1378470:intron 35.0%
TGTTGTTCCTAAACTTGCAT+AGG - tig0020061:55311-55330 None:intergenic 35.0%
GAACAACATGATAAGCTTGT+TGG + tig0020061:55323-55342 Msa1378470:intron 35.0%
!!! TTTTGGGATCACCATGAATA+TGG - tig0020061:55400-55419 None:intergenic 35.0%
AAGGGGTGAAAAATTAGAGT+AGG - tig0020061:55461-55480 None:intergenic 35.0%
! TACTCTAATTTTTCACCCCT+TGG + tig0020061:55460-55479 Msa1378470:intron 35.0%
! ACTCTAATTTTTCACCCCTT+GGG + tig0020061:55461-55480 Msa1378470:intron 35.0%
!!! TTTTTTTCCAAACCACCCAA+GGG - tig0020061:55479-55498 None:intergenic 35.0%
!!! ATTTTTTTCCAAACCACCCA+AGG - tig0020061:55480-55499 None:intergenic 35.0%
TAACAGAGAATGGTCAAAGA+TGG - tig0020061:55571-55590 None:intergenic 35.0%
TAGTATGTGCTTATTGTGCT+TGG + tig0020061:55029-55048 Msa1378470:intron 35.0%
CTATGCCTAATGTACATGTA+TGG + tig0020061:55056-55075 Msa1378470:intron 35.0%
!!! AACACTGGCCTTTTTAAGTT+AGG + tig0020061:55147-55166 Msa1378470:intron 35.0%
! GCGTTGATGAAATATATGAG+TGG + tig0020061:55242-55261 Msa1378470:intron 35.0%
TGTTGTTCCTAAACTTGCAT+AGG - tig0020061:55311-55330 None:intergenic 35.0%
GAACAACATGATAAGCTTGT+TGG + tig0020061:55323-55342 Msa1378470:intron 35.0%
!!! TTTTGGGATCACCATGAATA+TGG - tig0020061:55400-55419 None:intergenic 35.0%
AAGGGGTGAAAAATTAGAGT+AGG - tig0020061:55461-55480 None:intergenic 35.0%
! TACTCTAATTTTTCACCCCT+TGG + tig0020061:55460-55479 Msa1378470:intron 35.0%
! ACTCTAATTTTTCACCCCTT+GGG + tig0020061:55461-55480 Msa1378470:intron 35.0%
!!! TTTTTTTCCAAACCACCCAA+GGG - tig0020061:55479-55498 None:intergenic 35.0%
!!! ATTTTTTTCCAAACCACCCA+AGG - tig0020061:55480-55499 None:intergenic 35.0%
TAACAGAGAATGGTCAAAGA+TGG - tig0020061:55571-55590 None:intergenic 35.0%
TTGAGAAGCTAATTGGGAAG+AGG + tig0020061:54900-54919 Msa1378470:CDS 40.0%
! TTCCAATCACCCTTTTGTTG+TGG - tig0020061:54953-54972 None:intergenic 40.0%
ACAGACACCTATGCAAGTTT+AGG + tig0020061:55301-55320 Msa1378470:intron 40.0%
!!! TTTTTTCCAAACCACCCAAG+GGG - tig0020061:55478-55497 None:intergenic 40.0%
TATGCCAATGTCTATGATGC+AGG + tig0020061:55527-55546 Msa1378470:intron 40.0%
AATGCCTGCATCATAGACAT+TGG - tig0020061:55534-55553 None:intergenic 40.0%
! GGTCAAAGATGGACTTTCAA+AGG - tig0020061:55560-55579 None:intergenic 40.0%
TTGAGAAGCTAATTGGGAAG+AGG + tig0020061:54900-54919 Msa1378470:CDS 40.0%
! TTCCAATCACCCTTTTGTTG+TGG - tig0020061:54953-54972 None:intergenic 40.0%
ACAGACACCTATGCAAGTTT+AGG + tig0020061:55301-55320 Msa1378470:intron 40.0%
!!! TTTTTTCCAAACCACCCAAG+GGG - tig0020061:55478-55497 None:intergenic 40.0%
TATGCCAATGTCTATGATGC+AGG + tig0020061:55527-55546 Msa1378470:intron 40.0%
AATGCCTGCATCATAGACAT+TGG - tig0020061:55534-55553 None:intergenic 40.0%
! GGTCAAAGATGGACTTTCAA+AGG - tig0020061:55560-55579 None:intergenic 40.0%
TCAGTGCGGTAAATACCTGA+AGG - tig0020061:54786-54805 None:intergenic 45.0%
GTCCACAACAAAAGGGTGAT+TGG + tig0020061:54948-54967 Msa1378470:CDS 45.0%
! CTAATTTTTCACCCCTTGGG+TGG + tig0020061:55464-55483 Msa1378470:intron 45.0%
TCAGTGCGGTAAATACCTGA+AGG - tig0020061:54786-54805 None:intergenic 45.0%
GTCCACAACAAAAGGGTGAT+TGG + tig0020061:54948-54967 Msa1378470:CDS 45.0%
! CTAATTTTTCACCCCTTGGG+TGG + tig0020061:55464-55483 Msa1378470:intron 45.0%
!!! TTTTTTATGATAAATTTTTT+AGG + tig0020061:55673-55692 Msa1378470:intron 5.0%
!!! TTTTTTATGATAAATTTTTT+AGG + tig0020061:55673-55692 Msa1378470:intron 5.0%
! TTTTCACCCCTTGGGTGGTT+TGG + tig0020061:55469-55488 Msa1378470:intron 50.0%
! TTTTCACCCCTTGGGTGGTT+TGG + tig0020061:55469-55488 Msa1378470:intron 50.0%
Chromosome Type Strat End Strand Name
tig0020061 gene 54770 55751 54770 ID=Msa1378470;Name=Msa1378470
tig0020061 mRNA 54770 55751 54770 ID=Msa1378470-mRNA-1;Parent=Msa1378470;Name=Msa1378470-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|0.33|1|1|3|0|85
tig0020061 exon 54770 54789 54770 ID=Msa1378470-mRNA-1:exon:6063;Parent=Msa1378470-mRNA-1
tig0020061 exon 54850 55030 54850 ID=Msa1378470-mRNA-1:exon:6064;Parent=Msa1378470-mRNA-1
tig0020061 exon 55695 55751 55695 ID=Msa1378470-mRNA-1:exon:6065;Parent=Msa1378470-mRNA-1
tig0020061 CDS 54770 54789 54770 ID=Msa1378470-mRNA-1:cds;Parent=Msa1378470-mRNA-1
tig0020061 CDS 54850 55030 54850 ID=Msa1378470-mRNA-1:cds;Parent=Msa1378470-mRNA-1
tig0020061 CDS 55695 55751 55695 ID=Msa1378470-mRNA-1:cds;Parent=Msa1378470-mRNA-1
Gene Sequence

>Msa1378470

ATGTATATCTTTTCCTTCAGTAGTGGAGCAAAGAGATCATTGTTGAAATTGAAAGTAATGTTGAATGATTTTGAGAAGCTAATTGGGAAGAGGCCACAACAAGAGATGAAGAATTTAAGTCCACAACAAAAGGGTGATTGGAAAGACTTGTTTCTAATGAGTATTTCTTTTGCTGTTTATGTGTACATATCCCAGAAGTTAGATAAAGAATTCATATCCCAATCCAATACTGAGAAAATCTTTAGAGTGGTGAAATAA

Protein sequence

>Msa1378470

MYIFSFSSGAKRSLLKLKVMLNDFEKLIGKRPQQEMKNLSPQQKGDWKDLFLMSISFAVYVYISQKLDKEFISQSNTEKIFRVVK*