AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0714560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714560 MtrunA17_Chr5g0401781 95.261 211 7 2 1 211 1 208 5.54e-146 404
Msa0714560 MtrunA17_Chr4g0053351 75.132 189 45 2 20 208 3 189 6.82e-101 290
Msa0714560 MtrunA17_Chr4g0035151 62.842 183 62 1 13 195 5 181 8.02e-79 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714560 AT4G39220.2 65.730 178 61 0 28 205 13 190 7.48e-88 257
Msa0714560 AT4G39220.1 65.730 178 61 0 28 205 13 190 7.48e-88 257
Msa0714560 AT2G21600.1 59.487 195 77 2 17 211 3 195 1.22e-82 244
Msa0714560 AT2G23310.1 64.088 181 63 2 25 205 33 211 1.19e-78 234
Msa0714560 AT2G23310.2 64.088 181 62 3 25 205 33 210 1.08e-76 229
Msa0714560 AT2G18240.2 53.409 176 81 1 17 192 6 180 5.23e-63 195
Msa0714560 AT2G18240.1 53.409 176 81 1 17 192 6 180 5.40e-63 195

Find 111 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCATCTGCAAATAATAAA+AGG 0.169181 5_1:-7186292 None:intergenic
TTCCCTATTGGGCAACTATT+TGG 0.179428 5_1:+7186098 Msa0714560:three_prime_UTR
AATCCAAAATTAAATAAAAT+TGG 0.212256 5_1:-7186548 None:intergenic
TAAAATCATCATATATTAAA+AGG 0.263537 5_1:-7186355 None:intergenic
CTGGATGTTCAATTCCCTAT+TGG 0.268696 5_1:+7186086 Msa0714560:three_prime_UTR
GATGCCTTCGATGGCGGCTT+TGG 0.278485 5_1:+7186120 Msa0714560:three_prime_UTR
TAATGTGAAGCTACATGTTT+GGG 0.279412 5_1:-7185986 None:intergenic
GGCCTATACTCCTCTTCTAC+TGG 0.286487 5_1:+7185654 Msa0714560:CDS
AATCTCTTCGGTGGCGGTTC+CGG 0.307108 5_1:-7184194 None:intergenic
CGGCTCCATCACGCGCAAAT+TGG 0.307389 5_1:-7184134 None:intergenic
CGTTTGATATTCCAGTCTTC+TGG 0.311008 5_1:+7185633 Msa0714560:CDS
CATCCAAATAGTTGCCCAAT+AGG 0.316865 5_1:-7186101 None:intergenic
AACTCATCGGAAGCTCTAAT+AGG 0.321016 5_1:-7184484 None:intergenic
TATTACCAGAACTTGAATTC+AGG 0.325782 5_1:-7184529 None:intergenic
TGAATTCAGCAATAAGAGTT+TGG 0.333284 5_1:-7186228 None:intergenic
TTTCCCCTTTCTTTCTATAT+AGG 0.342052 5_1:+7186195 Msa0714560:three_prime_UTR
ATTTAAAAGTCTCAGATGAT+TGG 0.346254 5_1:-7186483 None:intergenic
TGGATGTTCAATTCCCTATT+GGG 0.350059 5_1:+7186087 Msa0714560:three_prime_UTR
TGTAGTATTGCGGTTTCCGC+TGG 0.355795 5_1:-7184220 None:intergenic
AGATATGTACCGTTCAATTT+CGG 0.357992 5_1:+7185731 Msa0714560:CDS
ATAATGTGAAGCTACATGTT+TGG 0.362106 5_1:-7185987 None:intergenic
TACAATTGAAACAAGCGATA+TGG 0.364821 5_1:+7184239 Msa0714560:CDS
ATGCCTTCGATGGCGGCTTT+GGG 0.380608 5_1:+7186121 Msa0714560:three_prime_UTR
GGCGGTTCCGGTGCCGGTGC+CGG 0.385511 5_1:-7184182 None:intergenic
TTCGGTGGCGGTTCCGGTGC+CGG 0.385777 5_1:-7184188 None:intergenic
ACCGTTCAATTTCGGAAAAC+AGG 0.388365 5_1:+7185739 Msa0714560:CDS
CTAGCGTATATGTGAACATC+TGG 0.408780 5_1:+7186067 Msa0714560:three_prime_UTR
TAAAATTGGCACAGTACAAC+TGG 0.419750 5_1:-7186534 None:intergenic
GCCTCCCTGAATTCAAGTTC+TGG 0.421292 5_1:+7184524 Msa0714560:CDS
CATCGGTTTCCTCTCCCCTC+AGG 0.422986 5_1:+7184420 Msa0714560:CDS
GCCTTCCTATATAGAAAGAA+AGG 0.426650 5_1:-7186200 None:intergenic
CTCCGCGTCTACTTCTTAGA+AGG 0.426734 5_1:+7184349 Msa0714560:CDS
ACTCATCGGAAGCTCTAATA+GGG 0.427636 5_1:-7184483 None:intergenic
GGCGGCTTTGGGAAGATGTT+GGG 0.434631 5_1:+7186132 Msa0714560:three_prime_UTR
TTCCGCTGGGGAAATCTCTT+CGG 0.439901 5_1:-7184206 None:intergenic
CCGGCGTCGACGACGACTGC+TGG 0.442121 5_1:-7184163 None:intergenic
ATCCAAATAGTTGCCCAATA+GGG 0.450449 5_1:-7186100 None:intergenic
TACGTCGTTACGTACGGTCT+TGG 0.457317 5_1:+7184376 Msa0714560:CDS
TCAGCGGCGTCGGCGATGGC+GGG 0.461503 5_1:-7184448 None:intergenic
CCCATCTGCAAATAATAAAA+GGG 0.463408 5_1:-7186291 None:intergenic
ATCCATCGCGGTAGCACGTA+CGG 0.464069 5_1:-7184295 None:intergenic
GTCAGCGGCGTCGGCGATGG+CGG 0.464499 5_1:-7184449 None:intergenic
AATAAAAGGGACTGCTGCAC+AGG 0.465889 5_1:-7186278 None:intergenic
ACCGTCAGCGGCGTCGGCGA+TGG 0.469840 5_1:-7184452 None:intergenic
CCATCACGCGCAAATTGGAT+TGG 0.473804 5_1:-7184129 None:intergenic
CAATTTGCGCGTGATGGAGC+CGG 0.476023 5_1:+7184135 Msa0714560:exon
AGGAGTATAGGCCAGAAGAC+TGG 0.477362 5_1:-7185644 None:intergenic
ATTACCAGAACTTGAATTCA+GGG 0.479649 5_1:-7184528 None:intergenic
GTCGACGCCGGCACCGGCAC+CGG 0.482106 5_1:+7184175 Msa0714560:CDS
GCCTATACTCCTCTTCTACT+GGG 0.482573 5_1:+7185655 Msa0714560:CDS
CGGGATCAACCTGAGGGGAG+AGG 0.483334 5_1:-7184429 None:intergenic
AACTATTTGGATGCCTTCGA+TGG 0.485046 5_1:+7186111 Msa0714560:three_prime_UTR
CCTTCCTATATAGAAAGAAA+GGG 0.485262 5_1:-7186199 None:intergenic
TGGCGGCTTTGGGAAGATGT+TGG 0.490480 5_1:+7186131 Msa0714560:three_prime_UTR
CCCTTTCTTTCTATATAGGA+AGG 0.492829 5_1:+7186199 Msa0714560:three_prime_UTR
TCCATCGCGGTAGCACGTAC+GGG 0.502160 5_1:-7184294 None:intergenic
CCAATCCAATTTGCGCGTGA+TGG 0.504986 5_1:+7184129 Msa0714560:exon
AACTGAAAATTTCAGTCCAC+AGG 0.507776 5_1:-7185935 None:intergenic
GACTGATTAAGATTGCTTTG+AGG 0.509371 5_1:+7186043 Msa0714560:three_prime_UTR
ACCCAGTAGAAGAGGAGTAT+AGG 0.512859 5_1:-7185656 None:intergenic
GGCTTCTACGTCGTTACGTA+CGG 0.512972 5_1:+7184370 Msa0714560:CDS
TACATCCTCAATCTTCTCAT+CGG 0.515635 5_1:+7184403 Msa0714560:CDS
ACGACGACTGCTGGTGCTGC+CGG 0.516904 5_1:-7184154 None:intergenic
GTAGTATTGCGGTTTCCGCT+GGG 0.520236 5_1:-7184219 None:intergenic
TACTTGTGGGATCTTACAAA+GGG 0.520960 5_1:-7184065 None:intergenic
GTCGTCGTCGACGCCGGCAC+CGG 0.524903 5_1:+7184169 Msa0714560:CDS
TGGGGAAATCTCTTCGGTGG+CGG 0.525533 5_1:-7184200 None:intergenic
ACAAGACCACCCAGTAGAAG+AGG 0.526419 5_1:-7185664 None:intergenic
GCCATCGCCGACGCCGCTGA+CGG 0.527187 5_1:+7184451 Msa0714560:CDS
TTGTTTCAATTGTAGTATTG+CGG 0.528509 5_1:-7184230 None:intergenic
AGTAGGACCGTCAGCGGCGT+CGG 0.528995 5_1:-7184458 None:intergenic
CAGAAATTAGAAATTACTTG+TGG 0.534249 5_1:-7184079 None:intergenic
TACGTGCTACCGCGATGGAT+AGG 0.536752 5_1:+7184298 Msa0714560:CDS
ACGTGCTACCGCGATGGATA+GGG 0.540998 5_1:+7184299 Msa0714560:CDS
CGCTGGGGAAATCTCTTCGG+TGG 0.542688 5_1:-7184203 None:intergenic
AGAAATTAGAAATTACTTGT+GGG 0.543292 5_1:-7184078 None:intergenic
TCAGGGAGGCGGCGAACGAA+AGG 0.547738 5_1:-7184511 None:intergenic
CCCCGTACGTGCTACCGCGA+TGG 0.554113 5_1:+7184293 Msa0714560:CDS
TCTTGTTCTCACTTACTATG+AGG 0.557944 5_1:+7185681 Msa0714560:CDS
CGATGGCGGGATCAACCTGA+GGG 0.571666 5_1:-7184435 None:intergenic
TTACTTGTGGGATCTTACAA+AGG 0.572790 5_1:-7184066 None:intergenic
CTTCCCAAAGCCGCCATCGA+AGG 0.572881 5_1:-7186124 None:intergenic
TATTTGGATGCCTTCGATGG+CGG 0.573269 5_1:+7186114 Msa0714560:three_prime_UTR
TCATCATATATTAAAAGGAA+AGG 0.574411 5_1:-7186350 None:intergenic
GCGATGGCGGGATCAACCTG+AGG 0.577682 5_1:-7184436 None:intergenic
ATTGGCACAGTACAACTGGT+TGG 0.579263 5_1:-7186530 None:intergenic
GGAAACCGATGAGAAGATTG+AGG 0.580080 5_1:-7184408 None:intergenic
GGAGGCGGCGAACGAAAGGA+CGG 0.592422 5_1:-7184507 None:intergenic
TGTTCTCACTTACTATGAGG+AGG 0.595668 5_1:+7185684 Msa0714560:CDS
CAAGTGAAAATAACTTAGAG+AGG 0.596897 5_1:-7186384 None:intergenic
CCAGCAGTCGTCGTCGACGC+CGG 0.606494 5_1:+7184163 Msa0714560:CDS
AGAACTTGAATTCAGGGAGG+CGG 0.613554 5_1:-7184522 None:intergenic
ACGCGCAAATTGGATTGGAT+CGG 0.613606 5_1:-7184124 None:intergenic
GAAGCTCTAATAGGGAGAGT+AGG 0.615236 5_1:-7184475 None:intergenic
TAGTATTGCGGTTTCCGCTG+GGG 0.617844 5_1:-7184218 None:intergenic
CTTCCTATATAGAAAGAAAG+GGG 0.625817 5_1:-7186198 None:intergenic
AAGTGAAAATAACTTAGAGA+GGG 0.627791 5_1:-7186383 None:intergenic
AGAAAGCACAAGTCTTCCTG+TGG 0.635231 5_1:+7185919 Msa0714560:CDS
AACGAAAGGACGGAACTCAT+CGG 0.637257 5_1:-7184497 None:intergenic
TATACTCCTCTTCTACTGGG+TGG 0.667420 5_1:+7185658 Msa0714560:CDS
TTGGATCTAGTGAATAAGAG+AGG 0.679417 5_1:-7186511 None:intergenic
AGGGAGAGTAGGACCGTCAG+CGG 0.683952 5_1:-7184464 None:intergenic
AGCCTTCTAAGAAGTAGACG+CGG 0.688638 5_1:-7184351 None:intergenic
CCATCGCGGTAGCACGTACG+GGG 0.693992 5_1:-7184293 None:intergenic
CACCGAAGAGATTTCCCCAG+CGG 0.694340 5_1:+7184204 Msa0714560:CDS
CTTCTAAGAAGTAGACGCGG+AGG 0.701782 5_1:-7184348 None:intergenic
AATGTGAAGCTACATGTTTG+GGG 0.712062 5_1:-7185985 None:intergenic
ACCAGAACTTGAATTCAGGG+AGG 0.721876 5_1:-7184525 None:intergenic
GATGGCGGGATCAACCTGAG+GGG 0.723070 5_1:-7184434 None:intergenic
ACCGAAAACCCTATCCATCG+CGG 0.766599 5_1:-7184307 None:intergenic
GGGCAGGTACTCGATCACAG+TGG 0.793101 5_1:+7185574 Msa0714560:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTAATTATAATTTTGTT+AGG + chr5_1:7184964-7184983 Msa0714560:intron 10.0%
!!! AAAAAAAGTATTTTACAAAA+GGG - chr5_1:7186176-7186195 None:intergenic 10.0%
!!! AAAAAAAAGTATTTTACAAA+AGG - chr5_1:7186177-7186196 None:intergenic 10.0%
!!! TTTTGTAATTTTTTTGTTAT+TGG - chr5_1:7186258-7186277 None:intergenic 10.0%
!! TAAAATCATCATATATTAAA+AGG - chr5_1:7186358-7186377 None:intergenic 10.0%
!!! TTTTAATATATGATGATTTT+AGG + chr5_1:7186357-7186376 Msa0714560:three_prime_UTR 10.0%
!!! TTTTTCTGATATAAATTTTA+TGG + chr5_1:7186444-7186463 Msa0714560:three_prime_UTR 10.0%
!! AATCCAAAATTAAATAAAAT+TGG - chr5_1:7186551-7186570 None:intergenic 10.0%
!! AGAAATTAGAAATTACTTGT+GGG - chr5_1:7184081-7184100 None:intergenic 20.0%
!! TGCATAATTATTGTTAAATG+TGG + chr5_1:7184736-7184755 Msa0714560:intron 20.0%
!!! TTCTCATCAAAATTTCTTTT+TGG - chr5_1:7184847-7184866 None:intergenic 20.0%
!! TCATCATATATTAAAAGGAA+AGG - chr5_1:7186353-7186372 None:intergenic 20.0%
!!! TTTTCTTATCTGTTATTTCA+TGG + chr5_1:7186411-7186430 Msa0714560:three_prime_UTR 20.0%
!!! GTGCCAATTTTATTTAATTT+TGG + chr5_1:7186545-7186564 Msa0714560:three_prime_UTR 20.0%
! CAGAAATTAGAAATTACTTG+TGG - chr5_1:7184082-7184101 None:intergenic 25.0%
! TTGTTTCAATTGTAGTATTG+CGG - chr5_1:7184233-7184252 None:intergenic 25.0%
! AATTATGCACAATATCATCA+AGG - chr5_1:7184725-7184744 None:intergenic 25.0%
! TATGGAAACAACTTATGATA+AGG + chr5_1:7185198-7185217 Msa0714560:intron 25.0%
!!! GTTGTTTTCATAAAGTATAC+TGG - chr5_1:7185259-7185278 None:intergenic 25.0%
! ATTAAGTTCTTTATCCAAAG+AGG + chr5_1:7185380-7185399 Msa0714560:intron 25.0%
!!! AGTTTCTTTTTCACTTAGTA+AGG + chr5_1:7185506-7185525 Msa0714560:intron 25.0%
!!! TTGTGTTGATTGTTGTTATA+AGG + chr5_1:7185816-7185835 Msa0714560:intron 25.0%
! AAGTGAAAATAACTTAGAGA+GGG - chr5_1:7186386-7186405 None:intergenic 25.0%
!!! TTTATATCAGAAAAAGTGCT+AGG - chr5_1:7186439-7186458 None:intergenic 25.0%
! ATTTAAAAGTCTCAGATGAT+TGG - chr5_1:7186486-7186505 None:intergenic 25.0%
TACAATTGAAACAAGCGATA+TGG + chr5_1:7184239-7184258 Msa0714560:CDS 30.0%
ATTACCAGAACTTGAATTCA+GGG - chr5_1:7184531-7184550 None:intergenic 30.0%
TATTACCAGAACTTGAATTC+AGG - chr5_1:7184532-7184551 None:intergenic 30.0%
TTTGTTAGGTAGTTGTTAGT+TGG + chr5_1:7184978-7184997 Msa0714560:intron 30.0%
TAACTACCATTGTATGTCAA+CGG + chr5_1:7185018-7185037 Msa0714560:intron 30.0%
AAAGTATACTGGATAGCTTA+TGG - chr5_1:7185248-7185267 None:intergenic 30.0%
CCTAAAACAACATATCAATG+TGG - chr5_1:7185423-7185442 None:intergenic 30.0%
!!! CCACATTGATATGTTGTTTT+AGG + chr5_1:7185420-7185439 Msa0714560:intron 30.0%
AGATATGTACCGTTCAATTT+CGG + chr5_1:7185731-7185750 Msa0714560:CDS 30.0%
TAATGTGAAGCTACATGTTT+GGG - chr5_1:7185989-7186008 None:intergenic 30.0%
ATAATGTGAAGCTACATGTT+TGG - chr5_1:7185990-7186009 None:intergenic 30.0%
TTTCCCCTTTCTTTCTATAT+AGG + chr5_1:7186195-7186214 Msa0714560:three_prime_UTR 30.0%
CTTCCTATATAGAAAGAAAG+GGG - chr5_1:7186201-7186220 None:intergenic 30.0%
CCTTCCTATATAGAAAGAAA+GGG - chr5_1:7186202-7186221 None:intergenic 30.0%
TGAATTCAGCAATAAGAGTT+TGG - chr5_1:7186231-7186250 None:intergenic 30.0%
! TCCCTTTTATTATTTGCAGA+TGG + chr5_1:7186290-7186309 Msa0714560:three_prime_UTR 30.0%
CCCATCTGCAAATAATAAAA+GGG - chr5_1:7186294-7186313 None:intergenic 30.0%
! CCCTTTTATTATTTGCAGAT+GGG + chr5_1:7186291-7186310 Msa0714560:three_prime_UTR 30.0%
! CCTTTTATTATTTGCAGATG+GGG + chr5_1:7186292-7186311 Msa0714560:three_prime_UTR 30.0%
AGGAAAGGAAAAAAATGCAA+TGG - chr5_1:7186338-7186357 None:intergenic 30.0%
CAAGTGAAAATAACTTAGAG+AGG - chr5_1:7186387-7186406 None:intergenic 30.0%
!! CTCTCTAAGTTATTTTCACT+TGG + chr5_1:7186385-7186404 Msa0714560:three_prime_UTR 30.0%
TACTTGTGGGATCTTACAAA+GGG - chr5_1:7184068-7184087 None:intergenic 35.0%
TTACTTGTGGGATCTTACAA+AGG - chr5_1:7184069-7184088 None:intergenic 35.0%
TACATCCTCAATCTTCTCAT+CGG + chr5_1:7184403-7184422 Msa0714560:CDS 35.0%
! ATCGTTTAGTTGAGCGATTT+TGG + chr5_1:7184572-7184591 Msa0714560:intron 35.0%
!! TAGTTGAGCGATTTTGGTAT+AGG + chr5_1:7184578-7184597 Msa0714560:intron 35.0%
TGACGGTAATGAATGATTGA+CGG - chr5_1:7184651-7184670 None:intergenic 35.0%
ACGAAGTGTGTATAAACTGA+CGG - chr5_1:7184668-7184687 None:intergenic 35.0%
! CAATCTAGCTTGCAATATCA+AGG - chr5_1:7184780-7184799 None:intergenic 35.0%
TTACATCCGTTGACATACAA+TGG - chr5_1:7185027-7185046 None:intergenic 35.0%
! TTCTCTTCCTTTTCTTCACA+GGG + chr5_1:7185107-7185126 Msa0714560:intron 35.0%
TCAAATAAACCAACCCAAAC+AGG - chr5_1:7185133-7185152 None:intergenic 35.0%
GTATGTGATGAGAGTTTACT+GGG + chr5_1:7185353-7185372 Msa0714560:intron 35.0%
CTAGATGATGTTGTCTTCTT+CGG + chr5_1:7185460-7185479 Msa0714560:intron 35.0%
TCTTGTTCTCACTTACTATG+AGG + chr5_1:7185681-7185700 Msa0714560:CDS 35.0%
! ACCTGTTTTCCGAAATTGAA+CGG - chr5_1:7185743-7185762 None:intergenic 35.0%
! TTGTTGTTGTGATTGTTGTG+TGG + chr5_1:7185778-7185797 Msa0714560:intron 35.0%
AACTGAAAATTTCAGTCCAC+AGG - chr5_1:7185938-7185957 None:intergenic 35.0%
AATGTGAAGCTACATGTTTG+GGG - chr5_1:7185988-7186007 None:intergenic 35.0%
GACTGATTAAGATTGCTTTG+AGG + chr5_1:7186043-7186062 Msa0714560:three_prime_UTR 35.0%
TGGATGTTCAATTCCCTATT+GGG + chr5_1:7186087-7186106 Msa0714560:three_prime_UTR 35.0%
ATCCAAATAGTTGCCCAATA+GGG - chr5_1:7186103-7186122 None:intergenic 35.0%
CCCTTTCTTTCTATATAGGA+AGG + chr5_1:7186199-7186218 Msa0714560:three_prime_UTR 35.0%
GCCTTCCTATATAGAAAGAA+AGG - chr5_1:7186203-7186222 None:intergenic 35.0%
CCCCATCTGCAAATAATAAA+AGG - chr5_1:7186295-7186314 None:intergenic 35.0%
TTGGATCTAGTGAATAAGAG+AGG - chr5_1:7186514-7186533 None:intergenic 35.0%
TAAAATTGGCACAGTACAAC+TGG - chr5_1:7186537-7186556 None:intergenic 35.0%
! ACTCATCGGAAGCTCTAATA+GGG - chr5_1:7184486-7184505 None:intergenic 40.0%
! AACTCATCGGAAGCTCTAAT+AGG - chr5_1:7184487-7184506 None:intergenic 40.0%
ACTTCGTTCTCGTGTGATTA+AGG + chr5_1:7184681-7184700 Msa0714560:intron 40.0%
AGAACCATAGAATGTGGTTG+AGG - chr5_1:7185091-7185110 None:intergenic 40.0%
AGGAAGAGAACCATAGAATG+TGG - chr5_1:7185097-7185116 None:intergenic 40.0%
! GTTCTCTTCCTTTTCTTCAC+AGG + chr5_1:7185106-7185125 Msa0714560:intron 40.0%
AGACTGTTTCAGAGAGCTTA+TGG + chr5_1:7185180-7185199 Msa0714560:intron 40.0%
CGTATGTGATGAGAGTTTAC+TGG + chr5_1:7185352-7185371 Msa0714560:intron 40.0%
ATCCAAAGAGGACCATAGTT+TGG + chr5_1:7185392-7185411 Msa0714560:intron 40.0%
TCCAAAGAGGACCATAGTTT+GGG + chr5_1:7185393-7185412 Msa0714560:intron 40.0%
AATGTGGAAGACCCAAACTA+TGG - chr5_1:7185407-7185426 None:intergenic 40.0%
GAAGACAACATCATCTAGGT+AGG - chr5_1:7185458-7185477 None:intergenic 40.0%
CGAAGAAGACAACATCATCT+AGG - chr5_1:7185462-7185481 None:intergenic 40.0%
! GATGTTGTCTTCTTCGGTTT+TGG + chr5_1:7185466-7185485 Msa0714560:intron 40.0%
! AGCTGCTCAATTTTCCTTTG+TGG + chr5_1:7185553-7185572 Msa0714560:intron 40.0%
! GCTGCTCAATTTTCCTTTGT+GGG + chr5_1:7185554-7185573 Msa0714560:intron 40.0%
CGTTTGATATTCCAGTCTTC+TGG + chr5_1:7185633-7185652 Msa0714560:CDS 40.0%
TGTTCTCACTTACTATGAGG+AGG + chr5_1:7185684-7185703 Msa0714560:CDS 40.0%
ACCGTTCAATTTCGGAAAAC+AGG + chr5_1:7185739-7185758 Msa0714560:CDS 40.0%
CTAGCGTATATGTGAACATC+TGG + chr5_1:7186067-7186086 Msa0714560:three_prime_UTR 40.0%
CTGGATGTTCAATTCCCTAT+TGG + chr5_1:7186086-7186105 Msa0714560:three_prime_UTR 40.0%
TTCCCTATTGGGCAACTATT+TGG + chr5_1:7186098-7186117 Msa0714560:three_prime_UTR 40.0%
CATCCAAATAGTTGCCCAAT+AGG - chr5_1:7186104-7186123 None:intergenic 40.0%
AACTATTTGGATGCCTTCGA+TGG + chr5_1:7186111-7186130 Msa0714560:three_prime_UTR 40.0%
! ACGCGCAAATTGGATTGGAT+CGG - chr5_1:7184127-7184146 None:intergenic 45.0%
! AGCCTTCTAAGAAGTAGACG+CGG - chr5_1:7184354-7184373 None:intergenic 45.0%
GGAAACCGATGAGAAGATTG+AGG - chr5_1:7184411-7184430 None:intergenic 45.0%
! GAAGCTCTAATAGGGAGAGT+AGG - chr5_1:7184478-7184497 None:intergenic 45.0%
AACGAAAGGACGGAACTCAT+CGG - chr5_1:7184500-7184519 None:intergenic 45.0%
AGAACTTGAATTCAGGGAGG+CGG - chr5_1:7184525-7184544 None:intergenic 45.0%
ACCAGAACTTGAATTCAGGG+AGG - chr5_1:7184528-7184547 None:intergenic 45.0%
TGCAAGCTAGATTGTGCATG+CGG + chr5_1:7184786-7184805 Msa0714560:intron 45.0%
GAGTCCTCAACCACATTCTA+TGG + chr5_1:7185084-7185103 Msa0714560:intron 45.0%
AAACAGGCCCTGTGAAGAAA+AGG - chr5_1:7185117-7185136 None:intergenic 45.0%
! TTTTCTTCACAGGGCCTGTT+TGG + chr5_1:7185116-7185135 Msa0714560:intron 45.0%
TTTCTTCACAGGGCCTGTTT+GGG + chr5_1:7185117-7185136 Msa0714560:intron 45.0%
ACCCAAACTATGGTCCTCTT+TGG - chr5_1:7185397-7185416 None:intergenic 45.0%
CACTTAGTAAGGTCTGCAGA+AGG + chr5_1:7185517-7185536 Msa0714560:intron 45.0%
! CTCAATTTTCCTTTGTGGGC+AGG + chr5_1:7185558-7185577 Msa0714560:intron 45.0%
GCCTATACTCCTCTTCTACT+GGG + chr5_1:7185655-7185674 Msa0714560:CDS 45.0%
! ACCCAGTAGAAGAGGAGTAT+AGG - chr5_1:7185659-7185678 None:intergenic 45.0%
TATACTCCTCTTCTACTGGG+TGG + chr5_1:7185658-7185677 Msa0714560:CDS 45.0%
! AGAAAGCACAAGTCTTCCTG+TGG + chr5_1:7185919-7185938 Msa0714560:CDS 45.0%
TATTTGGATGCCTTCGATGG+CGG + chr5_1:7186114-7186133 Msa0714560:three_prime_UTR 45.0%
AATAAAAGGGACTGCTGCAC+AGG - chr5_1:7186281-7186300 None:intergenic 45.0%
ATTGGCACAGTACAACTGGT+TGG - chr5_1:7186533-7186552 None:intergenic 45.0%
CCATCACGCGCAAATTGGAT+TGG - chr5_1:7184132-7184151 None:intergenic 50.0%
CCAATCCAATTTGCGCGTGA+TGG + chr5_1:7184129-7184148 Msa0714560:exon 50.0%
TTCCGCTGGGGAAATCTCTT+CGG - chr5_1:7184209-7184228 None:intergenic 50.0%
TAGTATTGCGGTTTCCGCTG+GGG - chr5_1:7184221-7184240 None:intergenic 50.0%
GTAGTATTGCGGTTTCCGCT+GGG - chr5_1:7184222-7184241 None:intergenic 50.0%
TGTAGTATTGCGGTTTCCGC+TGG - chr5_1:7184223-7184242 None:intergenic 50.0%
! ACCGCGATGGATAGGGTTTT+CGG + chr5_1:7184306-7184325 Msa0714560:CDS 50.0%
ACCGAAAACCCTATCCATCG+CGG - chr5_1:7184310-7184329 None:intergenic 50.0%
! CTTCTAAGAAGTAGACGCGG+AGG - chr5_1:7184351-7184370 None:intergenic 50.0%
CTCCGCGTCTACTTCTTAGA+AGG + chr5_1:7184349-7184368 Msa0714560:CDS 50.0%
GGCTTCTACGTCGTTACGTA+CGG + chr5_1:7184370-7184389 Msa0714560:CDS 50.0%
TACGTCGTTACGTACGGTCT+TGG + chr5_1:7184376-7184395 Msa0714560:CDS 50.0%
! GCCTCCCTGAATTCAAGTTC+TGG + chr5_1:7184524-7184543 Msa0714560:CDS 50.0%
! AGGAGTATAGGCCAGAAGAC+TGG - chr5_1:7185647-7185666 None:intergenic 50.0%
GGCCTATACTCCTCTTCTAC+TGG + chr5_1:7185654-7185673 Msa0714560:CDS 50.0%
ACAAGACCACCCAGTAGAAG+AGG - chr5_1:7185667-7185686 None:intergenic 50.0%
! CAATTTGCGCGTGATGGAGC+CGG + chr5_1:7184135-7184154 Msa0714560:exon 55.0%
! AATCTCTTCGGTGGCGGTTC+CGG - chr5_1:7184197-7184216 None:intergenic 55.0%
TGGGGAAATCTCTTCGGTGG+CGG - chr5_1:7184203-7184222 None:intergenic 55.0%
CACCGAAGAGATTTCCCCAG+CGG + chr5_1:7184204-7184223 Msa0714560:CDS 55.0%
ATCCATCGCGGTAGCACGTA+CGG - chr5_1:7184298-7184317 None:intergenic 55.0%
TACGTGCTACCGCGATGGAT+AGG + chr5_1:7184298-7184317 Msa0714560:CDS 55.0%
ACGTGCTACCGCGATGGATA+GGG + chr5_1:7184299-7184318 Msa0714560:CDS 55.0%
TTCACAGGGCCTGTTTGGGT+TGG + chr5_1:7185121-7185140 Msa0714560:intron 55.0%
GATCGAGTACCTGCCCACAA+AGG - chr5_1:7185570-7185589 None:intergenic 55.0%
! ATGCCTTCGATGGCGGCTTT+GGG + chr5_1:7186121-7186140 Msa0714560:three_prime_UTR 55.0%
!! TGGCGGCTTTGGGAAGATGT+TGG + chr5_1:7186131-7186150 Msa0714560:three_prime_UTR 55.0%
!! GGCGGCTTTGGGAAGATGTT+GGG + chr5_1:7186132-7186151 Msa0714560:three_prime_UTR 55.0%
CGGCTCCATCACGCGCAAAT+TGG - chr5_1:7184137-7184156 None:intergenic 60.0%
CGCTGGGGAAATCTCTTCGG+TGG - chr5_1:7184206-7184225 None:intergenic 60.0%
TCCATCGCGGTAGCACGTAC+GGG - chr5_1:7184297-7184316 None:intergenic 60.0%
!! CATCGGTTTCCTCTCCCCTC+AGG + chr5_1:7184420-7184439 Msa0714560:CDS 60.0%
!! GATGGCGGGATCAACCTGAG+GGG - chr5_1:7184437-7184456 None:intergenic 60.0%
!! CGATGGCGGGATCAACCTGA+GGG - chr5_1:7184438-7184457 None:intergenic 60.0%
AGGGAGAGTAGGACCGTCAG+CGG - chr5_1:7184467-7184486 None:intergenic 60.0%
GGGCAGGTACTCGATCACAG+TGG + chr5_1:7185574-7185593 Msa0714560:intron 60.0%
! GATGCCTTCGATGGCGGCTT+TGG + chr5_1:7186120-7186139 Msa0714560:three_prime_UTR 60.0%
CTTCCCAAAGCCGCCATCGA+AGG - chr5_1:7186127-7186146 None:intergenic 60.0%
!! ACGACGACTGCTGGTGCTGC+CGG - chr5_1:7184157-7184176 None:intergenic 65.0%
CCATCGCGGTAGCACGTACG+GGG - chr5_1:7184296-7184315 None:intergenic 65.0%
CGGGATCAACCTGAGGGGAG+AGG - chr5_1:7184432-7184451 None:intergenic 65.0%
!! GCGATGGCGGGATCAACCTG+AGG - chr5_1:7184439-7184458 None:intergenic 65.0%
AGTAGGACCGTCAGCGGCGT+CGG - chr5_1:7184461-7184480 None:intergenic 65.0%
GGAGGCGGCGAACGAAAGGA+CGG - chr5_1:7184510-7184529 None:intergenic 65.0%
TCAGGGAGGCGGCGAACGAA+AGG - chr5_1:7184514-7184533 None:intergenic 65.0%
CCAGCAGTCGTCGTCGACGC+CGG + chr5_1:7184163-7184182 Msa0714560:CDS 70.0%
! TTCGGTGGCGGTTCCGGTGC+CGG - chr5_1:7184191-7184210 None:intergenic 70.0%
CCCCGTACGTGCTACCGCGA+TGG + chr5_1:7184293-7184312 Msa0714560:CDS 70.0%
!! CCGGCGTCGACGACGACTGC+TGG - chr5_1:7184166-7184185 None:intergenic 75.0%
GTCGTCGTCGACGCCGGCAC+CGG + chr5_1:7184169-7184188 Msa0714560:CDS 75.0%
!! TCAGCGGCGTCGGCGATGGC+GGG - chr5_1:7184451-7184470 None:intergenic 75.0%
!! GTCAGCGGCGTCGGCGATGG+CGG - chr5_1:7184452-7184471 None:intergenic 75.0%
GCCATCGCCGACGCCGCTGA+CGG + chr5_1:7184451-7184470 Msa0714560:CDS 75.0%
!! ACCGTCAGCGGCGTCGGCGA+TGG - chr5_1:7184455-7184474 None:intergenic 75.0%
!!! GTCGACGCCGGCACCGGCAC+CGG + chr5_1:7184175-7184194 Msa0714560:CDS 80.0%
!!! GGCGGTTCCGGTGCCGGTGC+CGG - chr5_1:7184185-7184204 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 7184060 7186596 7184060 ID=Msa0714560;Name=Msa0714560
chr5_1 mRNA 7184060 7186596 7184060 ID=Msa0714560-mRNA-1;Parent=Msa0714560;Name=Msa0714560-mRNA-1;_AED=0.07;_eAED=0.07;_QI=88|1|1|1|1|1|3|650|211
chr5_1 exon 7184060 7184545 7184060 ID=Msa0714560-mRNA-1:exon:2977;Parent=Msa0714560-mRNA-1
chr5_1 exon 7185580 7185760 7185580 ID=Msa0714560-mRNA-1:exon:2978;Parent=Msa0714560-mRNA-1
chr5_1 exon 7185890 7186596 7185890 ID=Msa0714560-mRNA-1:exon:2979;Parent=Msa0714560-mRNA-1
chr5_1 five_prime_UTR 7184060 7184147 7184060 ID=Msa0714560-mRNA-1:five_prime_utr;Parent=Msa0714560-mRNA-1
chr5_1 CDS 7184148 7184545 7184148 ID=Msa0714560-mRNA-1:cds;Parent=Msa0714560-mRNA-1
chr5_1 CDS 7185580 7185760 7185580 ID=Msa0714560-mRNA-1:cds;Parent=Msa0714560-mRNA-1
chr5_1 CDS 7185890 7185946 7185890 ID=Msa0714560-mRNA-1:cds;Parent=Msa0714560-mRNA-1
chr5_1 three_prime_UTR 7185947 7186596 7185947 ID=Msa0714560-mRNA-1:three_prime_utr;Parent=Msa0714560-mRNA-1
Gene Sequence

>Msa0714560

ATGGAGCCGGCAGCACCAGCAGTCGTCGTCGACGCCGGCACCGGCACCGGAACCGCCACCGAAGAGATTTCCCCAGCGGAAACCGCAATACTACAATTGAAACAAGCGATATGGCAACAATATCAACACGTGCTAGACAAAACAACCCCGTACGTGCTACCGCGATGGATAGGGTTTTCGGTCGTCGCTTTCATCTACATCCTCCGCGTCTACTTCTTAGAAGGCTTCTACGTCGTTACGTACGGTCTTGGCATTTACATCCTCAATCTTCTCATCGGTTTCCTCTCCCCTCAGGTTGATCCCGCCATCGCCGACGCCGCTGACGGTCCTACTCTCCCTATTAGAGCTTCCGATGAGTTCCGTCCTTTCGTTCGCCGCCTCCCTGAATTCAAGTTCTGGTACTCGATCACAGTGGCATTTTGCATTGCAATTGTGATGACTTTCTTTAGTGCGTTTGATATTCCAGTCTTCTGGCCTATACTCCTCTTCTACTGGGTGGTCTTGTTCTCACTTACTATGAGGAGGCAGATATCACATATGATCAAATACAGATATGTACCGTTCAATTTCGGAAAACAGCACTATCAGAGAAAGAGAGCATCAGAAGAAGAAAGCACAAGTCTTCCTGTGGACTGA
Protein sequence

>Msa0714560

MEPAAPAVVVDAGTGTGTATEEISPAETAILQLKQAIWQQYQHVLDKTTPYVLPRWIGFSVVAFIYILRVYFLEGFYVVTYGLGIYILNLLIGFLSPQVDPAIADAADGPTLPIRASDEFRPFVRRLPEFKFWYSITVAFCIAIVMTFFSAFDIPVFWPILLFYWVVLFSLTMRRQISHMIKYRYVPFNFGKQHYQRKRASEEESTSLPVD*