AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0143140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0143140 MtrunA17_Chr7g0266171 98.367 245 4 0 1 245 1 245 7.41e-177 485
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0143140 AT3G52760.1 68.872 257 68 4 1 245 1 257 2.74e-122 348
Msa0143140 AT2G36300.1 67.300 263 60 5 1 245 1 255 2.29e-114 328

Find 69 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCACATGGTGGTTTCTT+TGG 0.086528 1_4:+2071813 Msa0143140:CDS
TGCGGGTCGAACTGGAAATT+TGG 0.234078 1_4:+2071716 Msa0143140:CDS
TTCCACATGGTGGTTTCTTT+GGG 0.250135 1_4:+2071814 Msa0143140:CDS
GGAACCACCACCGCCGATTC+CGG 0.282867 1_4:-2071436 None:intergenic
GAACCACCACCGCCGATTCC+GGG 0.362735 1_4:-2071435 None:intergenic
GTATTCATCCGGATCAAATC+TGG 0.368693 1_4:+2071499 Msa0143140:CDS
AACGCCATATAGAGAAGAAT+TGG 0.368721 1_4:-2071594 None:intergenic
TGGTCCAATTCTTCTCTATA+TGG 0.369922 1_4:+2071590 Msa0143140:CDS
CATACTTGTACCAGTGTTGT+TGG 0.376301 1_4:+2071744 Msa0143140:CDS
AACATGCTTGCGGGTCGAAC+TGG 0.383114 1_4:+2071708 Msa0143140:CDS
CGGTATGGTTCACGCGAAAC+GGG 0.383392 1_4:-2071545 None:intergenic
CTCCTCGTCGTCGAAGTTAA+CGG 0.422501 1_4:-2071457 None:intergenic
TTTGGTTGGAACGGTGGTAT+TGG 0.423341 1_4:-2071336 None:intergenic
ATGAAAGGGATGCCGGAGTT+TGG 0.440296 1_4:-2071354 None:intergenic
ACGGTATGGTTCACGCGAAA+CGG 0.443655 1_4:-2071546 None:intergenic
ATCTACTCTGGTCCCGGAAT+CGG 0.456452 1_4:+2071423 Msa0143140:CDS
CTTGTGGTTTCTCTTACTGA+TGG 0.457923 1_4:+2071888 Msa0143140:CDS
GGTCCCGGAATCGGCGGTGG+TGG 0.458366 1_4:+2071432 Msa0143140:CDS
TGGACCGGAGGAGGTGGAAA+CGG 0.462451 1_4:-2071400 None:intergenic
CACAAAGACGCTGATCTCTC+TGG 0.477534 1_4:+2071570 Msa0143140:CDS
GGAGGAGGTGGAAACGGCGG+CGG 0.482053 1_4:-2071394 None:intergenic
CCGACGGAGGCGAAGGATAC+CGG 0.487942 1_4:-2071276 None:intergenic
ATTCCGGGACCAGAGTAGAT+TGG 0.488100 1_4:-2071420 None:intergenic
TGGGCTACTAGAGCTTCAAC+GGG 0.501360 1_4:+2071864 Msa0143140:CDS
CGATGTCAAATGACATGAAA+GGG 0.504894 1_4:-2071368 None:intergenic
CCGGTATCCTTCGCCTCCGT+CGG 0.505190 1_4:+2071276 Msa0143140:CDS
TCTGGTCCCGGAATCGGCGG+TGG 0.508217 1_4:+2071429 Msa0143140:CDS
GTAGATTGGACCGGAGGAGG+TGG 0.509987 1_4:-2071406 None:intergenic
GCCGCCGTTTCCACCTCCTC+CGG 0.518829 1_4:+2071396 Msa0143140:CDS
ATGCCGGAGTTTGGTTGGAA+CGG 0.525630 1_4:-2071345 None:intergenic
TAGAGCTTCAACGGGGCTTG+TGG 0.525930 1_4:+2071872 Msa0143140:CDS
GGGGTTACCGACGGAGGCGA+AGG 0.531653 1_4:-2071283 None:intergenic
AAGGGATGCCGGAGTTTGGT+TGG 0.532020 1_4:-2071350 None:intergenic
CGACGAGCTCGGTATTCATC+CGG 0.535700 1_4:+2071488 Msa0143140:CDS
ATACCGAGCTCGTCGAGAAG+AGG 0.537974 1_4:-2071480 None:intergenic
GGTCCAATCTACTCTGGTCC+CGG 0.538685 1_4:+2071417 Msa0143140:CDS
CAGCGTCTTTGTGAACGGTA+TGG 0.542374 1_4:-2071560 None:intergenic
CCTTTCATGTCATTTGACAT+CGG 0.544673 1_4:+2071369 Msa0143140:CDS
TTGTCCGGCGGGGTTACCGA+CGG 0.546984 1_4:-2071292 None:intergenic
ATGGGCTACTAGAGCTTCAA+CGG 0.549815 1_4:+2071863 Msa0143140:CDS
TACTCTGGTCCCGGAATCGG+CGG 0.561190 1_4:+2071426 Msa0143140:CDS
TGTCTCTTTCAATTACTCGC+CGG 0.564214 1_4:+2071618 Msa0143140:CDS
GAGCCTCTTCTCGACGAGCT+CGG 0.565975 1_4:+2071477 Msa0143140:CDS
AAATGACATGAAAGGGATGC+CGG 0.570013 1_4:-2071361 None:intergenic
GCCTCCGTCGGTAACCCCGC+CGG 0.572655 1_4:+2071288 Msa0143140:CDS
TCCTCCGGTCCAATCTACTC+TGG 0.574768 1_4:+2071411 Msa0143140:CDS
AGAGTAGATTGGACCGGAGG+AGG 0.576450 1_4:-2071409 None:intergenic
CCGGAGTTTGGTTGGAACGG+TGG 0.579512 1_4:-2071342 None:intergenic
CCACCGTTCCAACCAAACTC+CGG 0.614609 1_4:+2071342 Msa0143140:CDS
GGGACCAGAGTAGATTGGAC+CGG 0.616175 1_4:-2071415 None:intergenic
GAGATCAGCGTCTTTGTGAA+CGG 0.616493 1_4:-2071565 None:intergenic
TGGTTATTCTATGTTGCCAG+TGG 0.618449 1_4:+2071764 Msa0143140:CDS
GGAACGGTGGTATTGGTACA+CGG 0.619478 1_4:-2071329 None:intergenic
CCGATGTCAAATGACATGAA+AGG 0.627556 1_4:-2071369 None:intergenic
GAAAGAATTCAAAGTCCCAC+CGG 0.628112 1_4:+2071257 Msa0143140:CDS
TCCGGCGGGGTTACCGACGG+AGG 0.630208 1_4:-2071289 None:intergenic
ACCAGAGTAGATTGGACCGG+AGG 0.633247 1_4:-2071412 None:intergenic
ACGGAGGCGAAGGATACCGG+TGG 0.636258 1_4:-2071273 None:intergenic
CATAGAATAACCAACAACAC+TGG 0.637689 1_4:-2071754 None:intergenic
TTCCGTTAACTTCGACGACG+AGG 0.637706 1_4:+2071455 Msa0143140:CDS
ATTGCAGAGAAAATCACCAC+TGG 0.645448 1_4:-2071780 None:intergenic
ACCGGAGGAGGTGGAAACGG+CGG 0.648522 1_4:-2071397 None:intergenic
AATCTGGAGCAAAATCAGAT+CGG 0.650716 1_4:+2071515 Msa0143140:CDS
AGCCCAAAGAAACCACCATG+TGG 0.671114 1_4:-2071816 None:intergenic
CGGAGGCGAAGGATACCGGT+GGG 0.687481 1_4:-2071272 None:intergenic
ATGGCTGTGATGAACATCGT+GGG 0.690522 1_4:+2071907 Msa0143140:CDS
GATGGCTGTGATGAACATCG+TGG 0.761743 1_4:+2071906 Msa0143140:CDS
GGGCTACTAGAGCTTCAACG+GGG 0.801660 1_4:+2071865 Msa0143140:CDS
ACGGTGGTATTGGTACACGG+CGG 0.809815 1_4:-2071326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCAATTCGGTGTGATTTT+AGG + chr1_4:2071645-2071664 Msa0143140:CDS 30.0%
CGATGTCAAATGACATGAAA+GGG - chr1_4:2071371-2071390 None:intergenic 35.0%
! CCTTTCATGTCATTTGACAT+CGG + chr1_4:2071369-2071388 Msa0143140:CDS 35.0%
! ATTTTGCTCCAGATTTGATC+CGG - chr1_4:2071510-2071529 None:intergenic 35.0%
! AATCTGGAGCAAAATCAGAT+CGG + chr1_4:2071515-2071534 Msa0143140:CDS 35.0%
TGGTCCAATTCTTCTCTATA+TGG + chr1_4:2071590-2071609 Msa0143140:CDS 35.0%
AACGCCATATAGAGAAGAAT+TGG - chr1_4:2071597-2071616 None:intergenic 35.0%
! CACACCGAATTGAATTTTTC+CGG - chr1_4:2071640-2071659 None:intergenic 35.0%
!!! AATTCGGTGTGATTTTAGGT+TGG + chr1_4:2071649-2071668 Msa0143140:CDS 35.0%
CATAGAATAACCAACAACAC+TGG - chr1_4:2071757-2071776 None:intergenic 35.0%
!!! ATATCGCTTTTTCTTCCACA+TGG + chr1_4:2071801-2071820 Msa0143140:CDS 35.0%
GAAAGAATTCAAAGTCCCAC+CGG + chr1_4:2071257-2071276 Msa0143140:CDS 40.0%
!! TTTTGAAGATTTTGTCCGGC+GGG - chr1_4:2071306-2071325 None:intergenic 40.0%
!!! GTTTTGAAGATTTTGTCCGG+CGG - chr1_4:2071307-2071326 None:intergenic 40.0%
!!! GCGGTTTTGAAGATTTTGTC+CGG - chr1_4:2071310-2071329 None:intergenic 40.0%
AAATGACATGAAAGGGATGC+CGG - chr1_4:2071364-2071383 None:intergenic 40.0%
CCGATGTCAAATGACATGAA+AGG - chr1_4:2071372-2071391 None:intergenic 40.0%
GTATTCATCCGGATCAAATC+TGG + chr1_4:2071499-2071518 Msa0143140:CDS 40.0%
TGTCTCTTTCAATTACTCGC+CGG + chr1_4:2071618-2071637 Msa0143140:CDS 40.0%
CTCGCCGGAAAAATTCAATT+CGG + chr1_4:2071633-2071652 Msa0143140:CDS 40.0%
!!! TGTGATTTTAGGTTGGATCG+TGG + chr1_4:2071656-2071675 Msa0143140:CDS 40.0%
!! CGTTGTTTTCAACATGCTTG+CGG + chr1_4:2071698-2071717 Msa0143140:CDS 40.0%
!! GTTGTTTTCAACATGCTTGC+GGG + chr1_4:2071699-2071718 Msa0143140:CDS 40.0%
CATACTTGTACCAGTGTTGT+TGG + chr1_4:2071744-2071763 Msa0143140:CDS 40.0%
TGGTTATTCTATGTTGCCAG+TGG + chr1_4:2071764-2071783 Msa0143140:CDS 40.0%
ATTGCAGAGAAAATCACCAC+TGG - chr1_4:2071783-2071802 None:intergenic 40.0%
TTCCACATGGTGGTTTCTTT+GGG + chr1_4:2071814-2071833 Msa0143140:CDS 40.0%
! TCGCTGCGATTTTTGTTCTA+TGG + chr1_4:2071844-2071863 Msa0143140:CDS 40.0%
! CGCTGCGATTTTTGTTCTAT+GGG + chr1_4:2071845-2071864 Msa0143140:CDS 40.0%
CTTGTGGTTTCTCTTACTGA+TGG + chr1_4:2071888-2071907 Msa0143140:CDS 40.0%
! TTTGAAGATTTTGTCCGGCG+GGG - chr1_4:2071305-2071324 None:intergenic 45.0%
TTTGGTTGGAACGGTGGTAT+TGG - chr1_4:2071339-2071358 None:intergenic 45.0%
GAGATCAGCGTCTTTGTGAA+CGG - chr1_4:2071568-2071587 None:intergenic 45.0%
!!! TCGCTTTTTCTTCCACATGG+TGG + chr1_4:2071804-2071823 Msa0143140:CDS 45.0%
CTTCCACATGGTGGTTTCTT+TGG + chr1_4:2071813-2071832 Msa0143140:CDS 45.0%
! ATGGGCTACTAGAGCTTCAA+CGG + chr1_4:2071863-2071882 Msa0143140:CDS 45.0%
ATGGCTGTGATGAACATCGT+GGG + chr1_4:2071907-2071926 Msa0143140:CDS 45.0%
!! GGAACGGTGGTATTGGTACA+CGG - chr1_4:2071332-2071351 None:intergenic 50.0%
ATGCCGGAGTTTGGTTGGAA+CGG - chr1_4:2071348-2071367 None:intergenic 50.0%
ATGAAAGGGATGCCGGAGTT+TGG - chr1_4:2071357-2071376 None:intergenic 50.0%
ATTCCGGGACCAGAGTAGAT+TGG - chr1_4:2071423-2071442 None:intergenic 50.0%
ATCTACTCTGGTCCCGGAAT+CGG + chr1_4:2071423-2071442 Msa0143140:CDS 50.0%
TTCCGTTAACTTCGACGACG+AGG + chr1_4:2071455-2071474 Msa0143140:CDS 50.0%
CTCCTCGTCGTCGAAGTTAA+CGG - chr1_4:2071460-2071479 None:intergenic 50.0%
ACGGTATGGTTCACGCGAAA+CGG - chr1_4:2071549-2071568 None:intergenic 50.0%
CAGCGTCTTTGTGAACGGTA+TGG - chr1_4:2071563-2071582 None:intergenic 50.0%
CACAAAGACGCTGATCTCTC+TGG + chr1_4:2071570-2071589 Msa0143140:CDS 50.0%
TGCGGGTCGAACTGGAAATT+TGG + chr1_4:2071716-2071735 Msa0143140:CDS 50.0%
AGCCCAAAGAAACCACCATG+TGG - chr1_4:2071819-2071838 None:intergenic 50.0%
TGGGCTACTAGAGCTTCAAC+GGG + chr1_4:2071864-2071883 Msa0143140:CDS 50.0%
GATGGCTGTGATGAACATCG+TGG + chr1_4:2071906-2071925 Msa0143140:CDS 50.0%
!! ACGGTGGTATTGGTACACGG+CGG - chr1_4:2071329-2071348 None:intergenic 55.0%
CCACCGTTCCAACCAAACTC+CGG + chr1_4:2071342-2071361 Msa0143140:CDS 55.0%
AAGGGATGCCGGAGTTTGGT+TGG - chr1_4:2071353-2071372 None:intergenic 55.0%
AGAGTAGATTGGACCGGAGG+AGG - chr1_4:2071412-2071431 None:intergenic 55.0%
TCCTCCGGTCCAATCTACTC+TGG + chr1_4:2071411-2071430 Msa0143140:CDS 55.0%
ACCAGAGTAGATTGGACCGG+AGG - chr1_4:2071415-2071434 None:intergenic 55.0%
GGGACCAGAGTAGATTGGAC+CGG - chr1_4:2071418-2071437 None:intergenic 55.0%
GGTCCAATCTACTCTGGTCC+CGG + chr1_4:2071417-2071436 Msa0143140:CDS 55.0%
ATACCGAGCTCGTCGAGAAG+AGG - chr1_4:2071483-2071502 None:intergenic 55.0%
CGACGAGCTCGGTATTCATC+CGG + chr1_4:2071488-2071507 Msa0143140:CDS 55.0%
CGGTATGGTTCACGCGAAAC+GGG - chr1_4:2071548-2071567 None:intergenic 55.0%
AACATGCTTGCGGGTCGAAC+TGG + chr1_4:2071708-2071727 Msa0143140:CDS 55.0%
GGGCTACTAGAGCTTCAACG+GGG + chr1_4:2071865-2071884 Msa0143140:CDS 55.0%
TAGAGCTTCAACGGGGCTTG+TGG + chr1_4:2071872-2071891 Msa0143140:CDS 55.0%
CCGGAGTTTGGTTGGAACGG+TGG - chr1_4:2071345-2071364 None:intergenic 60.0%
TGGACCGGAGGAGGTGGAAA+CGG - chr1_4:2071403-2071422 None:intergenic 60.0%
GTAGATTGGACCGGAGGAGG+TGG - chr1_4:2071409-2071428 None:intergenic 60.0%
TACTCTGGTCCCGGAATCGG+CGG + chr1_4:2071426-2071445 Msa0143140:CDS 60.0%
GAGCCTCTTCTCGACGAGCT+CGG + chr1_4:2071477-2071496 Msa0143140:CDS 60.0%
CGGAGGCGAAGGATACCGGT+GGG - chr1_4:2071275-2071294 None:intergenic 65.0%
ACGGAGGCGAAGGATACCGG+TGG - chr1_4:2071276-2071295 None:intergenic 65.0%
CCGACGGAGGCGAAGGATAC+CGG - chr1_4:2071279-2071298 None:intergenic 65.0%
CCGGTATCCTTCGCCTCCGT+CGG + chr1_4:2071276-2071295 Msa0143140:CDS 65.0%
TTGTCCGGCGGGGTTACCGA+CGG - chr1_4:2071295-2071314 None:intergenic 65.0%
ACCGGAGGAGGTGGAAACGG+CGG - chr1_4:2071400-2071419 None:intergenic 65.0%
!! GAACCACCACCGCCGATTCC+GGG - chr1_4:2071438-2071457 None:intergenic 65.0%
!! GGAACCACCACCGCCGATTC+CGG - chr1_4:2071439-2071458 None:intergenic 65.0%
GGGGTTACCGACGGAGGCGA+AGG - chr1_4:2071286-2071305 None:intergenic 70.0%
GGAGGAGGTGGAAACGGCGG+CGG - chr1_4:2071397-2071416 None:intergenic 70.0%
GCCGCCGTTTCCACCTCCTC+CGG + chr1_4:2071396-2071415 Msa0143140:CDS 70.0%
TCTGGTCCCGGAATCGGCGG+TGG + chr1_4:2071429-2071448 Msa0143140:CDS 70.0%
GCCTCCGTCGGTAACCCCGC+CGG + chr1_4:2071288-2071307 Msa0143140:CDS 75.0%
TCCGGCGGGGTTACCGACGG+AGG - chr1_4:2071292-2071311 None:intergenic 75.0%
GGTCCCGGAATCGGCGGTGG+TGG + chr1_4:2071432-2071451 Msa0143140:CDS 75.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 2071252 2071989 2071252 ID=Msa0143140;Name=Msa0143140
chr1_4 mRNA 2071252 2071989 2071252 ID=Msa0143140-mRNA-1;Parent=Msa0143140;Name=Msa0143140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|245
chr1_4 exon 2071252 2071989 2071252 ID=Msa0143140-mRNA-1:exon:755;Parent=Msa0143140-mRNA-1
chr1_4 CDS 2071252 2071989 2071252 ID=Msa0143140-mRNA-1:cds;Parent=Msa0143140-mRNA-1
Gene Sequence

>Msa0143140

ATGGAGAAAGAATTCAAAGTCCCACCGGTATCCTTCGCCTCCGTCGGTAACCCCGCCGGACAAAATCTTCAAAACCGCCGTGTACCAATACCACCGTTCCAACCAAACTCCGGCATCCCTTTCATGTCATTTGACATCGGCTCCGCCGCCGTTTCCACCTCCTCCGGTCCAATCTACTCTGGTCCCGGAATCGGCGGTGGTGGTTCCGTTAACTTCGACGACGAGGAGCCTCTTCTCGACGAGCTCGGTATTCATCCGGATCAAATCTGGAGCAAAATCAGATCGGTTCTTAACCCGTTTCGCGTGAACCATACCGTTCACAAAGACGCTGATCTCTCTGGTCCAATTCTTCTCTATATGGCGTTTTGTCTCTTTCAATTACTCGCCGGAAAAATTCAATTCGGTGTGATTTTAGGTTGGATCGTGGTTTCTTCGATCTTTTTGTACGTTGTTTTCAACATGCTTGCGGGTCGAACTGGAAATTTGGATCTACATACTTGTACCAGTGTTGTTGGTTATTCTATGTTGCCAGTGGTGATTTTCTCTGCAATATCGCTTTTTCTTCCACATGGTGGTTTCTTTGGGCTTGCTATCGCTGCGATTTTTGTTCTATGGGCTACTAGAGCTTCAACGGGGCTTGTGGTTTCTCTTACTGATGGCTGTGATGAACATCGTGGGTTGATAGCGTACGCGTGTTTCTTGATTTACACTCTGTTTTCGTTGCTTGTGATTTTCTAG

Protein sequence

>Msa0143140

MEKEFKVPPVSFASVGNPAGQNLQNRRVPIPPFQPNSGIPFMSFDIGSAAVSTSSGPIYSGPGIGGGGSVNFDDEEPLLDELGIHPDQIWSKIRSVLNPFRVNHTVHKDADLSGPILLYMAFCLFQLLAGKIQFGVILGWIVVSSIFLYVVFNMLAGRTGNLDLHTCTSVVGYSMLPVVIFSAISLFLPHGGFFGLAIAAIFVLWATRASTGLVVSLTDGCDEHRGLIAYACFLIYTLFSLLVIF*