AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0844260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0844260 MtrunA17_CPg0492961 98.765 81 1 0 51 131 52 132 7.49e-51 159
Msa0844260 MtrunA17_Chr4g0016641 96.154 52 2 0 1 52 751 802 7.84e-28 107
Msa0844260 MtrunA17_CPg0493041 96.154 52 2 0 1 52 751 802 7.84e-28 107
Msa0844260 MtrunA17_Chr1g0159041 97.222 36 1 0 1 36 209 244 1.95e-17 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0844260 ATCG00130.1 72.000 100 22 1 32 131 41 134 2.04e-41 136
Msa0844260 ATCG00190.1 90.385 52 5 0 1 52 748 799 3.13e-26 102

Find 32 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGAGCTATACTTGGGATTC+AGG 0.269809 5_4:-22608643 Msa0844260:CDS
CTACTTTCCTTAAGCGGGCC+CGG 0.340021 5_4:+22607896 None:intergenic
TAATTCAATTTATACAACTT+TGG 0.388293 5_4:-22607806 Msa0844260:CDS
AGATTATTACGAGCTATACT+TGG 0.409704 5_4:-22608651 Msa0844260:CDS
AGAAAGCCCGGGCCCGCTTA+AGG 0.414131 5_4:-22607903 Msa0844260:CDS
ACTTGTCTAAAACTACCTAT+AGG 0.437083 5_4:-22608603 Msa0844260:CDS
CGTTTCTACTTTCCTTAAGC+GGG 0.444690 5_4:+22607891 None:intergenic
GCGGATCGGTTTCGAGTGAA+TGG 0.446998 5_4:-22607865 Msa0844260:CDS
TTCAAAATTCAGAAGAACTA+CGG 0.449969 5_4:-22607948 Msa0844260:CDS
GGAAGCAGCCGTTGAACAGC+TGG 0.450450 5_4:-22607926 Msa0844260:CDS
ACTCGGCCCCTACCACCTAT+AGG 0.455800 5_4:+22608588 None:intergenic
TACTTTCCTTAAGCGGGCCC+GGG 0.461743 5_4:+22607897 None:intergenic
GCTCGTAATAATCTATCTTC+CGG 0.462719 5_4:+22608660 None:intergenic
TTGAACAGCTGGAGAAAGCC+CGG 0.472109 5_4:-22607915 Msa0844260:CDS
GGCTTTCTCCAGCTGTTCAA+CGG 0.504402 5_4:+22607918 None:intergenic
TCAAAATTCAGAAGAACTAC+GGG 0.510719 5_4:-22607947 Msa0844260:CDS
CCGTTTCTACTTTCCTTAAG+CGG 0.521089 5_4:+22607890 None:intergenic
AAAACTACCTATAGGTGGTA+GGG 0.530153 5_4:-22608595 Msa0844260:CDS
TAAAACTACCTATAGGTGGT+AGG 0.538804 5_4:-22608596 Msa0844260:CDS
TAATCTATCTTCCGGAGCAT+AGG 0.540322 5_4:+22608668 None:intergenic
TCTATCTTCCGGAGCATAGG+AGG 0.540370 5_4:+22608671 None:intergenic
GATTATTACGAGCTATACTT+GGG 0.558504 5_4:-22608650 Msa0844260:CDS
GCCGAGTTATTGATGTGAGA+TGG 0.559021 5_4:-22608572 Msa0844260:CDS
AAGTAGAAACGGAAGCGGAT+CGG 0.570689 5_4:-22607879 Msa0844260:CDS
GAAAGAATCCTCCTATGCTC+CGG 0.570879 5_4:-22608679 Msa0844260:CDS
CCGCTTAAGGAAAGTAGAAA+CGG 0.573518 5_4:-22607890 Msa0844260:CDS
TGAACAGCTGGAGAAAGCCC+GGG 0.575165 5_4:-22607914 Msa0844260:CDS
TCCATCTCACATCAATAACT+CGG 0.602104 5_4:+22608571 None:intergenic
AAACTACCTATAGGTGGTAG+GGG 0.618823 5_4:-22608594 Msa0844260:CDS
AAGGAAAGTAGAAACGGAAG+CGG 0.622485 5_4:-22607884 Msa0844260:CDS
GATTCAGGTATCGACCTCAA+AGG 0.672007 5_4:-22608628 Msa0844260:CDS
TGTCTAAAACTACCTATAGG+TGG 0.673297 5_4:-22608600 Msa0844260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTAATCAGAATTCTT+TGG - chr5_4:22608159-22608178 Msa0844260:intron 15.0%
!! CAATACAAGATTAAAATATT+TGG + chr5_4:22608241-22608260 None:intergenic 15.0%
!!! CAAATATTTTAATCTTGTAT+TGG - chr5_4:22608239-22608258 Msa0844260:intron 15.0%
!! TAATTCAATTTATACAACTT+TGG - chr5_4:22608628-22608647 Msa0844260:CDS 15.0%
!! ATAAATTAATCAGAATTCTT+TGG - chr5_4:22608159-22608178 Msa0844260:intron 15.0%
!! CAATACAAGATTAAAATATT+TGG + chr5_4:22608241-22608260 None:intergenic 15.0%
!!! CAAATATTTTAATCTTGTAT+TGG - chr5_4:22608239-22608258 Msa0844260:intron 15.0%
!! TAATTCAATTTATACAACTT+TGG - chr5_4:22608628-22608647 Msa0844260:CDS 15.0%
!! ATATATATATGAATCGTTTC+TGG + chr5_4:22607918-22607937 None:intergenic 20.0%
!! ATTCTGATTAATTTATACGT+AGG + chr5_4:22608155-22608174 None:intergenic 20.0%
!! TACAAGATTAAAATATTTGG+AGG + chr5_4:22608238-22608257 None:intergenic 20.0%
!! ATATATATATGAATCGTTTC+TGG + chr5_4:22607918-22607937 None:intergenic 20.0%
!! ATTCTGATTAATTTATACGT+AGG + chr5_4:22608155-22608174 None:intergenic 20.0%
!! TACAAGATTAAAATATTTGG+AGG + chr5_4:22608238-22608257 None:intergenic 20.0%
! TGAAATTAAAGTTTCCTTTG+AGG - chr5_4:22607950-22607969 Msa0844260:CDS 25.0%
! GAAATTAAAGTTTCCTTTGA+GGG - chr5_4:22607951-22607970 Msa0844260:CDS 25.0%
!!! ATAAAGCAAATCTTGGTTTT+CGG - chr5_4:22608028-22608047 Msa0844260:intron 25.0%
! TTCTCTTTACGATGAAAAAT+AGG - chr5_4:22608077-22608096 Msa0844260:intron 25.0%
! TTTGCTGACAATTATTTGTT+TGG - chr5_4:22608202-22608221 Msa0844260:intron 25.0%
! TGTTTAGAAATAGAGAAAAG+AGG - chr5_4:22608277-22608296 Msa0844260:intron 25.0%
! CTCATTCGATAATAAAGATT+GGG - chr5_4:22608305-22608324 Msa0844260:intron 25.0%
! CATGAATCTTTCAATTCATT+TGG + chr5_4:22608361-22608380 None:intergenic 25.0%
! TGAATTGAAAGATTCATGTT+TGG - chr5_4:22608363-22608382 Msa0844260:intron 25.0%
! TTCAAAATTCAGAAGAACTA+CGG - chr5_4:22608486-22608505 Msa0844260:intron 25.0%
! TGAAATTAAAGTTTCCTTTG+AGG - chr5_4:22607950-22607969 Msa0844260:CDS 25.0%
! GAAATTAAAGTTTCCTTTGA+GGG - chr5_4:22607951-22607970 Msa0844260:CDS 25.0%
!!! ATAAAGCAAATCTTGGTTTT+CGG - chr5_4:22608028-22608047 Msa0844260:intron 25.0%
! TTCTCTTTACGATGAAAAAT+AGG - chr5_4:22608077-22608096 Msa0844260:intron 25.0%
! TTTGCTGACAATTATTTGTT+TGG - chr5_4:22608202-22608221 Msa0844260:intron 25.0%
! TGTTTAGAAATAGAGAAAAG+AGG - chr5_4:22608277-22608296 Msa0844260:intron 25.0%
! CTCATTCGATAATAAAGATT+GGG - chr5_4:22608305-22608324 Msa0844260:intron 25.0%
! CATGAATCTTTCAATTCATT+TGG + chr5_4:22608361-22608380 None:intergenic 25.0%
! TGAATTGAAAGATTCATGTT+TGG - chr5_4:22608363-22608382 Msa0844260:intron 25.0%
! TTCAAAATTCAGAAGAACTA+CGG - chr5_4:22608486-22608505 Msa0844260:intron 25.0%
AGATTATTACGAGCTATACT+TGG - chr5_4:22607783-22607802 Msa0844260:CDS 30.0%
GATTATTACGAGCTATACTT+GGG - chr5_4:22607784-22607803 Msa0844260:CDS 30.0%
! TTTTAGACAAGTTTCCTTTG+AGG + chr5_4:22607823-22607842 None:intergenic 30.0%
! ACTTGTCTAAAACTACCTAT+AGG - chr5_4:22607831-22607850 Msa0844260:CDS 30.0%
! AAACCAAGATTTGCTTTATC+CGG + chr5_4:22608027-22608046 None:intergenic 30.0%
!! TCAAGTCATTTTATCGACAT+GGG + chr5_4:22608056-22608075 None:intergenic 30.0%
!! TTCAAGTCATTTTATCGACA+TGG + chr5_4:22608057-22608076 None:intergenic 30.0%
CGATGAAAAATAGGAAAAAC+AGG - chr5_4:22608086-22608105 Msa0844260:intron 30.0%
TCAATTCCGCATTGTATAAA+AGG + chr5_4:22608129-22608148 None:intergenic 30.0%
AGAAATAGAGAAAAGAGGAT+AGG - chr5_4:22608282-22608301 Msa0844260:intron 30.0%
GCTCATTCGATAATAAAGAT+TGG - chr5_4:22608304-22608323 Msa0844260:intron 30.0%
AGATTGGGAAATTTCCTATA+CGG - chr5_4:22608320-22608339 Msa0844260:intron 30.0%
TGAAAGATTCATGTTTGGTT+CGG - chr5_4:22608368-22608387 Msa0844260:intron 30.0%
TCAAAATTCAGAAGAACTAC+GGG - chr5_4:22608487-22608506 Msa0844260:intron 30.0%
! CAATTTTTCTCGTTCTATCT+CGG + chr5_4:22608600-22608619 None:intergenic 30.0%
! AACGATTCATTTTGAACAAC+AGG - chr5_4:22608676-22608695 Msa0844260:CDS 30.0%
! ACGATTCATTTTGAACAACA+GGG - chr5_4:22608677-22608696 Msa0844260:CDS 30.0%
AGATTATTACGAGCTATACT+TGG - chr5_4:22607783-22607802 Msa0844260:CDS 30.0%
GATTATTACGAGCTATACTT+GGG - chr5_4:22607784-22607803 Msa0844260:CDS 30.0%
! TTTTAGACAAGTTTCCTTTG+AGG + chr5_4:22607823-22607842 None:intergenic 30.0%
! ACTTGTCTAAAACTACCTAT+AGG - chr5_4:22607831-22607850 Msa0844260:CDS 30.0%
! AAACCAAGATTTGCTTTATC+CGG + chr5_4:22608027-22608046 None:intergenic 30.0%
!! TCAAGTCATTTTATCGACAT+GGG + chr5_4:22608056-22608075 None:intergenic 30.0%
!! TTCAAGTCATTTTATCGACA+TGG + chr5_4:22608057-22608076 None:intergenic 30.0%
CGATGAAAAATAGGAAAAAC+AGG - chr5_4:22608086-22608105 Msa0844260:intron 30.0%
TCAATTCCGCATTGTATAAA+AGG + chr5_4:22608129-22608148 None:intergenic 30.0%
AGAAATAGAGAAAAGAGGAT+AGG - chr5_4:22608282-22608301 Msa0844260:intron 30.0%
GCTCATTCGATAATAAAGAT+TGG - chr5_4:22608304-22608323 Msa0844260:intron 30.0%
AGATTGGGAAATTTCCTATA+CGG - chr5_4:22608320-22608339 Msa0844260:intron 30.0%
TGAAAGATTCATGTTTGGTT+CGG - chr5_4:22608368-22608387 Msa0844260:intron 30.0%
TCAAAATTCAGAAGAACTAC+GGG - chr5_4:22608487-22608506 Msa0844260:intron 30.0%
! CAATTTTTCTCGTTCTATCT+CGG + chr5_4:22608600-22608619 None:intergenic 30.0%
! AACGATTCATTTTGAACAAC+AGG - chr5_4:22608676-22608695 Msa0844260:CDS 30.0%
! ACGATTCATTTTGAACAACA+GGG - chr5_4:22608677-22608696 Msa0844260:CDS 30.0%
GCTCGTAATAATCTATCTTC+CGG + chr5_4:22607777-22607796 None:intergenic 35.0%
! TGTCTAAAACTACCTATAGG+TGG - chr5_4:22607834-22607853 Msa0844260:CDS 35.0%
TAAAACTACCTATAGGTGGT+AGG - chr5_4:22607838-22607857 Msa0844260:CDS 35.0%
AAAACTACCTATAGGTGGTA+GGG - chr5_4:22607839-22607858 Msa0844260:CDS 35.0%
TCCATCTCACATCAATAACT+CGG + chr5_4:22607866-22607885 None:intergenic 35.0%
AGATGGATCCAAAAAAAAGC+AGG - chr5_4:22607879-22607898 Msa0844260:CDS 35.0%
!!! AGCTAGAACCTGCTTTTTTT+TGG + chr5_4:22607890-22607909 None:intergenic 35.0%
!! AGGGTATTGATACTCCTAAA+AGG - chr5_4:22607970-22607989 Msa0844260:CDS 35.0%
! GAGCAAATAGAACTCCTTTT+AGG + chr5_4:22607987-22608006 None:intergenic 35.0%
ATACCGGATAAAGCAAATCT+TGG - chr5_4:22608021-22608040 Msa0844260:intron 35.0%
ATTCCGCATTGTATAAAAGG+AGG + chr5_4:22608126-22608145 None:intergenic 35.0%
! GAAAGATTCATGTTTGGTTC+GGG - chr5_4:22608369-22608388 Msa0844260:intron 35.0%
GCTCGTAATAATCTATCTTC+CGG + chr5_4:22607777-22607796 None:intergenic 35.0%
! TGTCTAAAACTACCTATAGG+TGG - chr5_4:22607834-22607853 Msa0844260:CDS 35.0%
TAAAACTACCTATAGGTGGT+AGG - chr5_4:22607838-22607857 Msa0844260:CDS 35.0%
AAAACTACCTATAGGTGGTA+GGG - chr5_4:22607839-22607858 Msa0844260:CDS 35.0%
TCCATCTCACATCAATAACT+CGG + chr5_4:22607866-22607885 None:intergenic 35.0%
AGATGGATCCAAAAAAAAGC+AGG - chr5_4:22607879-22607898 Msa0844260:CDS 35.0%
!!! AGCTAGAACCTGCTTTTTTT+TGG + chr5_4:22607890-22607909 None:intergenic 35.0%
!! AGGGTATTGATACTCCTAAA+AGG - chr5_4:22607970-22607989 Msa0844260:CDS 35.0%
! GAGCAAATAGAACTCCTTTT+AGG + chr5_4:22607987-22608006 None:intergenic 35.0%
ATACCGGATAAAGCAAATCT+TGG - chr5_4:22608021-22608040 Msa0844260:intron 35.0%
ATTCCGCATTGTATAAAAGG+AGG + chr5_4:22608126-22608145 None:intergenic 35.0%
! GAAAGATTCATGTTTGGTTC+GGG - chr5_4:22608369-22608388 Msa0844260:intron 35.0%
TAATCTATCTTCCGGAGCAT+AGG + chr5_4:22607769-22607788 None:intergenic 40.0%
AAACTACCTATAGGTGGTAG+GGG - chr5_4:22607840-22607859 Msa0844260:CDS 40.0%
!! AGGAGTATCAATACCCTCAA+AGG + chr5_4:22607967-22607986 None:intergenic 40.0%
! ACGCCTCCTTTTATACAATG+CGG - chr5_4:22608120-22608139 Msa0844260:intron 40.0%
TCTCACACTCAATTCCGTAT+AGG + chr5_4:22608337-22608356 None:intergenic 40.0%
CGTTTCTACTTTCCTTAAGC+GGG + chr5_4:22608546-22608565 None:intergenic 40.0%
CCGTTTCTACTTTCCTTAAG+CGG + chr5_4:22608547-22608566 None:intergenic 40.0%
CCGCTTAAGGAAAGTAGAAA+CGG - chr5_4:22608544-22608563 Msa0844260:intron 40.0%
AAGGAAAGTAGAAACGGAAG+CGG - chr5_4:22608550-22608569 Msa0844260:intron 40.0%
TAATCTATCTTCCGGAGCAT+AGG + chr5_4:22607769-22607788 None:intergenic 40.0%
AAACTACCTATAGGTGGTAG+GGG - chr5_4:22607840-22607859 Msa0844260:CDS 40.0%
!! AGGAGTATCAATACCCTCAA+AGG + chr5_4:22607967-22607986 None:intergenic 40.0%
! ACGCCTCCTTTTATACAATG+CGG - chr5_4:22608120-22608139 Msa0844260:intron 40.0%
TCTCACACTCAATTCCGTAT+AGG + chr5_4:22608337-22608356 None:intergenic 40.0%
CGTTTCTACTTTCCTTAAGC+GGG + chr5_4:22608546-22608565 None:intergenic 40.0%
CCGTTTCTACTTTCCTTAAG+CGG + chr5_4:22608547-22608566 None:intergenic 40.0%
CCGCTTAAGGAAAGTAGAAA+CGG - chr5_4:22608544-22608563 Msa0844260:intron 40.0%
AAGGAAAGTAGAAACGGAAG+CGG - chr5_4:22608550-22608569 Msa0844260:intron 40.0%
GAAAGAATCCTCCTATGCTC+CGG - chr5_4:22607755-22607774 Msa0844260:CDS 45.0%
ACGAGCTATACTTGGGATTC+AGG - chr5_4:22607791-22607810 Msa0844260:CDS 45.0%
!! GATTCAGGTATCGACCTCAA+AGG - chr5_4:22607806-22607825 Msa0844260:CDS 45.0%
GCCGAGTTATTGATGTGAGA+TGG - chr5_4:22607862-22607881 Msa0844260:CDS 45.0%
TCGTAGCTCACGATTGATAC+CGG - chr5_4:22608005-22608024 Msa0844260:intron 45.0%
AAGTAGAAACGGAAGCGGAT+CGG - chr5_4:22608555-22608574 Msa0844260:intron 45.0%
GAAAGAATCCTCCTATGCTC+CGG - chr5_4:22607755-22607774 Msa0844260:CDS 45.0%
ACGAGCTATACTTGGGATTC+AGG - chr5_4:22607791-22607810 Msa0844260:CDS 45.0%
!! GATTCAGGTATCGACCTCAA+AGG - chr5_4:22607806-22607825 Msa0844260:CDS 45.0%
GCCGAGTTATTGATGTGAGA+TGG - chr5_4:22607862-22607881 Msa0844260:CDS 45.0%
TCGTAGCTCACGATTGATAC+CGG - chr5_4:22608005-22608024 Msa0844260:intron 45.0%
AAGTAGAAACGGAAGCGGAT+CGG - chr5_4:22608555-22608574 Msa0844260:intron 45.0%
TCTATCTTCCGGAGCATAGG+AGG + chr5_4:22607766-22607785 None:intergenic 50.0%
GGCTTTCTCCAGCTGTTCAA+CGG + chr5_4:22608519-22608538 None:intergenic 50.0%
TTGAACAGCTGGAGAAAGCC+CGG - chr5_4:22608519-22608538 Msa0844260:intron 50.0%
TCTATCTTCCGGAGCATAGG+AGG + chr5_4:22607766-22607785 None:intergenic 50.0%
GGCTTTCTCCAGCTGTTCAA+CGG + chr5_4:22608519-22608538 None:intergenic 50.0%
TTGAACAGCTGGAGAAAGCC+CGG - chr5_4:22608519-22608538 Msa0844260:intron 50.0%
TGAACAGCTGGAGAAAGCCC+GGG - chr5_4:22608520-22608539 Msa0844260:intron 55.0%
TACTTTCCTTAAGCGGGCCC+GGG + chr5_4:22608540-22608559 None:intergenic 55.0%
CTACTTTCCTTAAGCGGGCC+CGG + chr5_4:22608541-22608560 None:intergenic 55.0%
! GCGGATCGGTTTCGAGTGAA+TGG - chr5_4:22608569-22608588 Msa0844260:CDS 55.0%
TGAACAGCTGGAGAAAGCCC+GGG - chr5_4:22608520-22608539 Msa0844260:intron 55.0%
TACTTTCCTTAAGCGGGCCC+GGG + chr5_4:22608540-22608559 None:intergenic 55.0%
CTACTTTCCTTAAGCGGGCC+CGG + chr5_4:22608541-22608560 None:intergenic 55.0%
! GCGGATCGGTTTCGAGTGAA+TGG - chr5_4:22608569-22608588 Msa0844260:CDS 55.0%
ACTCGGCCCCTACCACCTAT+AGG + chr5_4:22607849-22607868 None:intergenic 60.0%
GGAAGCAGCCGTTGAACAGC+TGG - chr5_4:22608508-22608527 Msa0844260:intron 60.0%
ACTCGGCCCCTACCACCTAT+AGG + chr5_4:22607849-22607868 None:intergenic 60.0%
GGAAGCAGCCGTTGAACAGC+TGG - chr5_4:22608508-22608527 Msa0844260:intron 60.0%
AGAAAGCCCGGGCCCGCTTA+AGG - chr5_4:22608531-22608550 Msa0844260:intron 65.0%
AGAAAGCCCGGGCCCGCTTA+AGG - chr5_4:22608531-22608550 Msa0844260:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 22607750 22608706 22607750 ID=Msa0844260;Name=Msa0844260
chr5_4 mRNA 22607750 22608706 22607750 ID=Msa0844260-mRNA-1;Parent=Msa0844260;Name=Msa0844260-mRNA-1;_AED=0.37;_eAED=0.39;_QI=0|0|0|1|0|0|2|0|133
chr5_4 exon 22608556 22608706 22608556 ID=Msa0844260-mRNA-1:exon:9275;Parent=Msa0844260-mRNA-1
chr5_4 exon 22607750 22608000 22607750 ID=Msa0844260-mRNA-1:exon:9274;Parent=Msa0844260-mRNA-1
chr5_4 CDS 22608556 22608706 22608556 ID=Msa0844260-mRNA-1:cds;Parent=Msa0844260-mRNA-1
chr5_4 CDS 22607750 22608000 22607750 ID=Msa0844260-mRNA-1:cds;Parent=Msa0844260-mRNA-1
Gene Sequence

>Msa0844260

ATGGCGAAAGAATCCTCCTATGCTCCGGAAGATAGATTATTACGAGCTATACTTGGGATTCAGGTATCGACCTCAAAGGAAACTTGTCTAAAACTACCTATAGGTGGTAGGGGCCGAGTTATTGATGTGAGATGGATCCAAAAAAAAGCAGATAATCGAAAACAGAGAATCTTGAGAACTATTCAAAATTCAGAAGAACTACGGGAAGCAGCCGTTGAACAGCTGGAGAAAGCCCGGGCCCGCTTAAGGAAAGTAGAAACGGAAGCGGATCGGTTTCGAGTGAATGGTTATTCCGAGATAGAACGAGAAAAATTGAATTTAATTAATTCAATTTATACAACTTTGGAACAATTCGAAAATTACAAAAATGAAACGATTCATTTTGAACAACAGGGCGATTAA

Protein sequence

>Msa0844260

MAKESSYAPEDRLLRAILGIQVSTSKETCLKLPIGGRGRVIDVRWIQKKADNRKQRILRTIQNSEELREAAVEQLEKARARLRKVETEADRFRVNGYSEIEREKLNLINSIYTTLEQFENYKNETIHFEQQGD*